How to use calcium imaging for neuroresearch?
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Choose the appropriate calcium ion indicator Calcium ion indicators are divided into chemiluminescent indicators and GECI indicators. Among them, GECI indicators, GCaMP6f and GCaMP6s, are widely used in in vivo calcium imaging research.
Transport the calcium ion indicator to the target The chemiluminescent indicator is usually pierced by an electrode or enter the target site by means of acetoxymethyl and dextrose, while the GECI indicator is introduced to the target site mainly by viral infection.
Collect signals and record the results Calcium imaging technology is usually used in conjunction with microscopy to record changes in calcium ions.
Fig.10 In Vivo Calcium Imaging
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Calcium Imaging