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Alternative Toxicity Services
We provide a high content screening service for evidence based acute general toxicity and neurotoxicity. We exploit alternative test methods in respect of the international principles of the 3Rs - Replacement, Refinement and Reduction. Our predictive technology can be used to test toxicity and assess safety of chemicals.
In vitro assay techniques • Mid-throughput electrophysiological recording of in vitro neuronal cultures coupled to micro electrode array (MEA) • Single-cell electrophysiology (Patch Clamp technique) • Single-cell Calcium Imaging
• Mid-throughput evaluation of compounds effect on neuronal networks • Cell-based assays and microscopy image-based analysis • Determination of Dose-Response or Dose-Inhibition profiles
• Characterization of compound pharmacological profiles at single-cell level • Evaluation of the neural differentiation potential of Stem Cells and iPS cells. Complementary tests on cell viability and cytotoxicity are
performed by LDH cytotoxicity assay and Mitochondrial Apoptosis Assay. We provide reliable and accurate data to fasten early stage drug development and minimize the risk of unpredicted and undetected toxicity side effects.
Alternative Toxicity Service complementary platforms of assays for in vitro toxicity testing Assay Format MEA electrophysiology
Patch Clamp electrophysiology
Ca2+ Imaging
LDH Assay
Mitochondrial Apoptosis Assay
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Human SH-SY5Y Neuroblastoma cell line
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Rat or Mouse Neonatal Cortical Astrocytes
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Rat or Mouse Adult Cortical Astrocytes
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Mouse BV2 Microglia cell line
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Rat or Mouse Neurotoxicity Embryonic (E18) Neurons Rat or Mouse Neonatal Neurons
Glia Toxicity
Cell models ETT offers a large range of models for evaluating general cell toxicity and neurotoxicity of test compounds, as • Rat or Mouse embryonic (E18) primary cortical neurons • Rat or Mouse neonatal primary cortical neurons
• Human SH-SY5Y Neuroblastoma cell line • Rat or Mouse Neonatal Cortical Astrocytes • Rat or Mouse Adult Cortical Astrocytes • Mouse BV2 Microglia cell line. All protocols are optimized to obtain
and maintain cell cultures in good and stable condition, in order to guarantee accurate and reliable information about effects of chemicals. We keep developing and adopting models more relevant for human safety.
Alternative Toxicity Services
MEA Mid-throughput electrophysiological recording ETT provides you with an innovative system to detect in vitro toxicity under respect of the 3R-principle. The ETT in vitro alternative assays provide evidence based toxicity data relevant to the new European Union Regulation REACH
(Registration, Evaluation, Authorisation and restriction of CHemicals) on chemicals and their safe use (EC 1907/2006). By means of MultiElectrode Array (MEA) technique, we investigate on different
neuronal properties such as neuronal firing frequency, bursting behavior and synaptic transmission, for toxicological screening.
MEA technique also provides fundamental information for assessing efficacy of neuro-targeted new drugs during preclinical development and testing.
Alternative Toxicity Services
Single-cell electrophysiology - Patch Clamp ETT assesses single cell electrophysiology and ion channel functions in response to compound interaction by Patch Clamp technique.
Patch Clamp is a highly accurate and sensitive evaluation method and provides complementary information to MEA based electrophysiology.
Calcium signaling, cell viability and citotoxicity Cellular Toxicity is evaluated by studying uncontrolled increase in cytoplasmic calcium. Quantitative image analysis on fluorescent Ca2+ indicator Fura 2-AM is used to asses dose-response curves. ETT provides its clients a complete report for
the best chemical profiling. Toxicity and safety screening reports include information about cell viability and cytotoxicity assessed by: • lactate dehydrogenase (LDH) assay
• Mithochondrial activity assay • Customized cell staining.
Alternative Toxicity Services
Consulting ETT supports its client with the world class consulting service for: • Standardized Operating Procedure (SOP) and Good Laboratory Practice (GLP) design and implementation
• In vitro cell culture developmental characterization • Laboratory set up • Training on cell culture methods and electrophysiological recordings.
Genoa Head Office Via Sestri, 37 16154 Genoa tel +39 010 6519116 fax +39 010 6518540 Branches: Rome, Milan, London, Pescara, Ancona, Palermo contacts@alternativetoxservice.com tel. +39 366 7889656
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