Plasticware Kits CS-06-001-00 CS-01-001-00 CS-03-001-00
3D InSight™ Microtissues
GravityPLUS™ and GravityTRAP™ Kit incl. research license (10x96) GravityTRAP™ Plate only (non-adhesive) (10x96 ) GravityPLUS™ Plate only (10x96)
3D InSight™ Maintenance Medium CS-07-001-01 CS-07-001-02 CS-07-002-01 CS-07-002-02 CS-07-101-01 CS-07-101-02 CS-07-101-03
Human Liver Rat Liver Cell Line
Maintenance Medium (500ml) Maintenance Medium (250ml) Maintenance Medium (500ml) Maintenance Medium (250ml) Maintenance Medium (500ml) Maintenance Medium (250ml) Maintenance Medium (125ml)
3D InSight™ Microtissues MT-01-001-01 MT-01-001-02 MT-01-001-12 MT-01-001-03 MT-01-001-13 MT-01-001-04 MT-01-001-14 MT-01-021-01 MT-01-021-02 MT-01-021-12 MT-01-021-03 MT-01-021-13 MT-01-021-04 MT-01-021-14 MT-01-002-01 MT-01-002-02 MT-01-002-12 MT-01-002-03 MT-01-002-13 MT-01-002-04 MT-01-002-14 MT-01-003-01 MT-01-003-02 MT-01-003-12 MT-01-003-03 MT-01-003-13 MT-01-003-04 MT-01-003-14 MT-01-014-01 MT-01-014-02 MT-01-014-12 MT-01-014-03 MT-01-014-13 MT-01-014-04 MT-01-014-14 MT-01-016-01 MT-01-016-02 MT-01-016-12 MT-01-016-03 MT-01-016-13 MT-01-016-04 MT-01-016-14 MT-01-017-01 MT-01-017-02 MT-01-017-12 MT-01-017-03 MT-01-017-13 MT-01-017-04 MT-01-017-14 MT-10-003-01 MT-10-003-03
Human Kidney Carcinoma
Human Prostate Carcinoma
Human Colorectal Carcinoma
Microtissues A498 (96x) co-culture microtissues A498 +3T3 (96x) co-culture microtissues A498 +3T3 +HUVEC (96x) co-culture microtissues A498 +NHDF (96x) co-culture microtissues A498 +NHDF +HUVEC (96x) co-culture microtissues A498 +MRC-5 (96x) co-culture microtissues A498 +MRC-5 +HUVEC (96x) Microtissues 786-O (96x) co-culture microtissues 786-O +3T3 (96x) co-culture microtissues 786-O +3T3 +HUVEC (96x) co-culture microtissues 786-O +NHDF (96x) co-culture microtissues 786-O +NHDF +HUVEC (96x) co-culture microtissues 786-O +MRC-5 (96x) co-culture microtissues 786-O +MRC-5 +HUVEC (96x) Microtissues DU145 (96x) co-culture microtissues DU145 +3T3 (96x) co-culture microtissues DU145 +3T3 +HUVEC (96x) co-culture microtissues DU145 +NHDF (96x) co-culture microtissues DU145 +NHDF +HUVEC (96x) co-culture microtissues DU145 +MRC-5 (96x) co-culture microtissues DU145 +MRC-5 +HUVEC (96x) Microtissues HCT116 (96x) co-culture microtissues HCT116 +3T3 (96x) co-culture microtissues HCT116 +3T3 +HUVEC (96x) co-culture microtissues HCT116 +NHDF (96x) co-culture microtissues HCT116 +NHDF +HUVEC (96x) co-culture microtissues HCT116 +MRC-5 (96x) co-culture microtissues HCT116 +MRC-5 +HUVEC (96x) Microtissues LoVo (96x) co-culture microtissues LoVo +3T3 (96x) co-culture microtissues LoVo +3T3 +HUVEC (96x) co-culture microtissues LoVo +NHDF (96x) co-culture microtissues LoVo +NHDF +HUVEC (96x) co-culture microtissues LoVo +MRC-5 (96x) co-culture microtissues LoVo +MRC-5 +HUVEC (96x) Microtissues DLD-1 (96x) co-culture microtissues DLD-1 + 3T3 (96x) co-culture microtissues DLD-1 +3T3 +HUVEC (96x) co-culture microtissues DLD-1 +NHDF (96x) co-culture microtissues DLD-1 +NHDF +HUVEC (96x) co-culture microtissues DLD-1 +MRC-5 (96x) co-culture microtissues DLD-1 +MRC-5 +HUVEC (96x) Microtissues LS174T (96x) co-culture microtissues LS174T + 3T3 (96x) co-culture microtissues LS174T +3T3 +HUVEC (96x) co-culture microtissues LS174T +NHDF (96x) co-culture microtissues LS174T +NHDF +HUVEC (96x) co-culture microtissues LS174T +MRC-5 (96x) co-culture microtissues LS174T +MRC-5 +HUVEC (96x) Microtissues HCT116 with GFP expression Microtissues HCT116 with GFP expression co-cultured with RFP-expressing NIH3T3
Colon Microtissues HT29 (96x) MT-01-004-01 Human Carcinoma
MT-01-005-01 MT-01-005-02 MT-01-005-12 MT-01-005-03 MT-01-005-13 MT-01-005-04 MT-01-005-14 MT-01-015-01 MT-01-015-02 MT-01-015-03 MT-01-015-04 MT-01-006-01 MT-01-006-02 MT-01-006-12 MT-01-006-03 MT-01-006-13 MT-01-006-04 MT-01-006-14
Human Liver Carcinoma
Human CNS Carcinoma
Microtissues HepG2 (96x) co-culture microtissues HepG2 +3T3 (96x) co-culture microtissues HepG2 +3T3 +HUVEC (96x) co-culture microtissues HepG2 +NHDF (96x) co-culture microtissues HepG2 +NHDF +HUVEC (96x) co-culture microtissues HepG2 +MRC-5 (96x) co-culture microtissues HepG2 +MRC-5 +HUVEC (96x) Microtissues Huh-7 (96x) co-culture microtissues Huh-7 +3T3 (96x) co-culture microtissues Huh-7 +NHDF (96x) co-culture microtissues Huh-7 +MRC-5 (96x) Microtissues SNB19 (96x) co-culture microtissues SNB19 +3T3 (96x) co-culture microtissues SNB19 +3T3 +HUVEC (96x) co-culture microtissues SNB19 +NHDF (96x) co-culture microtissues SNB19 +NHDF +HUVEC (96x) co-culture microtissues SNB19 +MRC-5 (96x) co-culture microtissues SNB19 +MRC-5 +HUVEC (96x)
MT-01-020-01 MT-01-020-02 MT-01-020-12 MT-01-020-03 MT-01-020-13 MT-01-020-04 MT-01-020-14 MT-01-007-01 MT-01-007-02 MT-01-007-12 MT-01-007-03 MT-01-007-13 MT-01-007-04 MT-01-007-14 MT-01-013-02 MT-01-013-03 MT-01-013-04 MT-01-008-01 MT-01-008-02 MT-01-008-12 MT-01-008-03 MT-01-008-13 MT-01-008-04 MT-01-008-14 MT-01-009-02 MT-01-009-12 MT-01-009-03 MT-01-009-13 MT-01-009-04 MT-01-009-14 MT-01-012-02 MT-01-012-12 MT-01-012-03 MT-01-012-13 MT-01-012-04 MT-01-012-14 MT-01-018-01 MT-01-018-02 MT-01-018-12 MT-01-018-03 MT-01-018-13 MT-01-018-04 MT-01-018-14 MT-01-019-01 MT-01-019-02 MT-01-019-12 MT-01-019-03 MT-01-019-13 MT-01-019-04 MT-01-019-14 MT-01-010-01 MT-01-011-02 MT-01-011-12 MT-01-011-03 MT-01-011-13 MT-01-011-04 MT-01-011-14 MT-01-022-01 MT-01-022-02 MT-01-022-12 MT-01-022-03 MT-01-022-13 MT-01-022-04 MT-01-022-14 MT-01-023-01 MT-01-023-02 MT-01-023-12 MT-01-023-03 MT-01-023-13 MT-01-023-04 MT-01-023-14 MT-01-024-01 MT-01-024-02 MT-01-024-12 MT-01-024-03 MT-01-024-13 MT-01-024-04 MT-01-024-14 MT-02-001-01 MT-02-001-04 MT-02-002-01 MT-02-002-04 MT-02-100-01
Human CNS Carcinoma
Human Lung Carcinoma
Human Ovarian Carcinoma
Human Breast Carcinoma
Human Neuroepithelioma
Human Cervix Carcinoma
Human Osteosarcoma
Rat liver Human Liver HepG2 liver
Microtissues LN-18 (96x) co-culture microtissues LN-18 +3T3 (96x) co-culture microtissues LN-18 +3T3 +HUVEC (96x) co-culture microtissues LN-18 +NHDF (96x) co-culture microtissues LN-18 +NHDF +HUVEC (96x) co-culture microtissues LN-18 +MRC-5 (96x) co-culture microtissues LN-18 +MRC-5 +HUVEC (96x) Microtissues A549 (96x) co-culture microtissues A549 +3T3 (96x) co-culture microtissues A549 +3T3 +HUVEC (96x) co-culture microtissues A549 +NHDF (96x) co-culture microtissues A549 +NHDF +HUVEC (96x) co-culture microtissues A549 +MRC-5 (96x) co-culture microtissues A549 +MRC-5 +HUVEC (96x) co-culture microtissues Colo699 +3T3 (96x) co-culture microtissues Colo699 +NHDF (96x) co-culture microtissues Colo699 +MRC-5 (96x) Microtissues A2780 (96x) co-culture microtissues A2780 + 3T3 (96x) co-culture microtissues A2780 +3T3 +HUVEC (96x) co-culture microtissues A2780 +NHDF (96x) co-culture microtissues A2780 +NHDF +HUVEC (96x) co-culture microtissues A2780 +MRC-5 (96x) co-culture microtissues A2780 +MRC-5 +HUVEC (96x) co-culture microtissues MDA-MB-231 +3T3 (96x) co-culture microtissues MDA-MB-231 +3T3 +HUVEC (96x) co-culture microtissues MDA-MB-231 +NHDF (96x) co-culture microtissues MDA-MB-231 +NHDF +HUVEC (96x) co-culture microtissues MDA-MB-231 +MRC-5 (96x) co-culture microtissues MDA-MB-231 +MRC-5 +HUVEC (96x) co-culture microtissues MCF-7 +3T3 (96x) co-culture microtissues MCF-7 +3T3 +HUVEC (96x) co-culture microtissues MCF-7 +NHDF (96x) co-culture microtissues MCF-7 +NHDF +HUVEC (96x) co-culture microtissues MCF-7 +MRC-5 (96x) co-culture microtissues MCF-7 +MRC-5 +HUVEC (96x) Microtissues T47-D (96x) co-culture microtissues T47-D +3T3 (96x) co-culture microtissues T47-D +3T3 +HUVEC (96x) co-culture microtissues T47-D +NHDF (96x) co-culture microtissues T47-D +NHDF +HUVEC (96x) co-culture microtissues T47-D +MRC-5 (96x) co-culture microtissues T47-D +MRC-5 +HUVEC (96x) Microtissues MDA-MB-361 (96x) co-culture microtissues MDA-MB-361 +3T3 (96x) co-culture microtissues MDA-MB-361 +3T3 +HUVEC (96x) co-culture microtissues MDA-MB-361 +NHDF (96x) co-culture microtissues MDA-MB-361 +NHDF +HUVEC (96x) co-culture microtissues MDA-MB-361 +MRC-5 (96x) co-culture microtissues MDA-MB-361 +MRC-5 +HUVEC (96x) Microtissues SK-N-MC (96x) co-culture microtissues C33-A +3T3 (96x) co-culture microtissues C33-A +3T3 +HUVEC (96x) co-culture microtissues C33-A +NHDF (96x) co-culture microtissues C33-A +NHDF +HUVEC (96x) co-culture microtissues C33-A +MRC-5 (96x) co-culture microtissues C33-A +MRC-5 +HUVEC (96x) Microtissues SAO-2 (96x) co-culture microtissues SAO-2 +3T3 (96x) co-culture microtissues SAO-2 +3T3 +HUVEC (96x) co-culture microtissues SAO-2 +NHDF (96x) co-culture microtissues SAO-2 +NHDF +HUVEC (96x) co-culture microtissues SAO-2 +MRC-5 (96x) co-culture microtissues SAO-2 +MRC-5 +HUVEC (96x) Microtissues HOS (96x) co-culture microtissues HOS +3T3 (96x) co-culture microtissues HOS +3T3 +HUVEC (96x) co-culture microtissues HOS +NHDF (96x) co-culture microtissues HOS +NHDF +HUVEC (96x) co-culture microtissues HOS +MRC-5 (96x) co-culture microtissues HOS +MRC-5 +HUVEC (96x) Microtissues MG-63 (96x) co-culture microtissues MG-63 +3T3 (96x) co-culture microtissues MG-63 +3T3 +HUVEC (96x) co-culture microtissues MG-63 +NHDF (96x) co-culture microtissues MG-63 +NHDF +HUVEC (96x) co-culture microtissues MG-63 +MRC-5 (96x) co-culture microtissues MG-63 +MRC-5 +HUVEC (96x) Microtissues formed by primary hepatocytes (96x) Microtissues from primary hepatocytes, co-culture with nonparenchymal cells (96x) Microtissues from primary hepatocytes (96x) Microtissues from primary hepatocytes, co-culture with Kupffer cells (96x) HepG2 liver microtissues (96x)
서울특별시 서초구 양재동 205-1 성운빌딩 2층 Tel : 02-3672-3188 Fax 02-3672-3187 hdbio@hdbio.net
Why is 3D Cell Culture System Used? Monolayer
Spheroid model
Native tumor perfused capillary
secondary necrosis Heterogeneous cell population Diffusion required High production of ECM
secondary necrosis Light-sheet microscopy (mDSLM) image. Maximu Maximum m projection projection of 2000 cells based b d on 310 stacks t k off 1 μm. Draq5 D 5 nuclear l staining t i i (red) ( d) and d MitoView Green itochondria staining (green).
Homogenous cell population No diffusion of substrates required Low to now production of ECM
3D microtissues “To make or Buy”
More in-vivo like… •세계 최초 co-culture system을 도입한 Ready-to-use microtissue 판매 •더 이상 Cell culture할 필요 없음 •이제는 cell이 아닌 tissue가 대세 •그 동안 경험 할 수 없었던 predictive power •Scaffold-Free, 96well strip format •Max. 35일까지 culture 가능 (5x longer life time) •Reference: pFizer, Cyprotex, Promega, Perkin Elmer
SureDrop™ inlet Lid(C)
GravityPLUS™
Raster Ra plate (B) with strips Bottom Bot plate(A)
GravityTRAP™
Cell Seeding
Microtissue Maturation
Microtissue Transfer
Microtissue Culture/Assay
서울특별시 서초구 양재동 205-1 성운빌딩 2층 Tel : 02-3672-3188 Fax 02-3672-3187 hdbio@hdbio.net
Channel(D) Reservoir(E)