Biod 171 Final Exam Nucleus - ✔Contains genomic Endoplasmic Reticulum - ✔Series of membrane-enclosed sacs and interconnected tubes Lysosomes - ✔contain hydrolytic enzymes for digestion Chloroplasts - ✔Site of photosynthesis Briefly explain how a Scanning Electron Microscope ( SEM ) forms an image of subcellular organelles , substrates , and viral particles . - ✔An image is formed from the interactions of electrons as they reflect off ( not through ) the surface of the specimen Samples are coated in either gold or palladium to enhance electron reflections . The reflection is seen in 3 - D rendering . Scalded Skin Syndrome - ✔Staph aureus Bubonic plague - ✔Yersina pestis Opthalmia Neonatorium - ✔Staphylococcus Legionnaires - ✔Legionella E. Coli - ✔Prokaryote Archaeons - ✔Prokaryote Yeast - ✔Eukaryote Amoeba - ✔Eukaryote Phototroph - ✔Utilize photons to Lithotroph - ✔Remove electrons from inorganic molecules Autotroph - ✔Obtain their source of carbon from inorganic molecules Chemotroph - ✔Acquire energy from
A researcher is looking to determine the source of contamination of a patient that presented with symptoms of food poisoning . To best determine between E. coli or Salmonella as the cause , what type of agar will be used to isolate the growth of each ? - ✔Differential Media Place the following viral life cycle steps in order beginning with viral attachment ( 3points ) and provide a description of " ENTRY " ( 1 point ) . - ✔1. Attachment 2. Entry 3. Uncoating 4. Replication 5. Release 6. New infection The Gram stain differentiates between bacteria based on the composition of their ✔Cell Wall Left untreated , three percent of cases of Streptococcal pharyngitis will progress to which of the following ? - ✔Rheumatic fever Describe TWO differences between DNA and RNA . - ✔RNA contains the sugar ribose rather than deoxyribose . RNA is always single - stranded , whereas DNA is double stranded True or False When grown on Blood Agar Plates ( BAP ) , microbes capable of alpha hemolysis present as greenish - brown color colonies due to incomplete ( partial ) lysis of red blood cells - ✔True Each of the following statements about glycolysis is true , except : - ✔The net gain is 4 ATP Describe the ONE main differences between lytic and temperate phages . - ✔Lytic bacteriophages replicate within the host bacteria until it ruptures whereas temperate ( or lysogenic ) phages primarily exist in a non - replicative state that does not kill the host cell . Bacteria that have a curved rod morphology are referred to as - ✔Vibrio You want to observe the movement of microorganisms that are present in a sample of cheek cells obtained on a patient . Briefly explain the technique that you would use and how it is performed - ✔A wet mount stain is indicated . A small liquid culture ( usually just a drop ) containing a microorganism of interest is prepared , added to a slide , and then covered with a glass coverslip . The coverslip is present to both protect the objective and the specimen while also holding the microorganism in place . Wet mounts are used to observe the motility and behavior of an organism . What coronavirus - associated infection gave rise to quarantining and travel restrictions in 2003 ? - ✔SARS
A multi - purpose media capable of growing a wide variety of microorganisms ✔Trypticase Soy Agar Also known as lysogeny broth , it is a multi - purpose media capable of growing a wide variety of microorganisms . - ✔LB Agar Pale red in color and is both selective and differential media . The presence of crystal violet and bile salts in its formulation restricts Gram positive bacterial growth . As such , only Gram negative microbes can be grown . - ✔MacConkey Agar This Agar is selective for Gram - positive bacteria and can differentiate members of the Staphylococci family . - ✔Mannitol Salt Agar True or False Leprosy can move through the blood to other areas including the brain , spine , and kidneys . - ✔False : TB is primarily considered a respiratory infection , but once it fully colonizes the lungs , TB can move through the blood to other areas including the brain , spine , and kidneys . True or False Proteins can function to catalyze biochemical processes and play a role in structure , but they cannot enable movement . - ✔False . Proteins can function to catalyze biochemical processes , play a role in structure , and they can enable movement You are working with a patient who claims a friend " got the COVID - 19 infection from the vaccine " . Explain why the viral vector vaccine used to prevent the COVID - 19 infection cannot transmit the actual infection . - ✔The genome in the viral vector only encodes for a spike protein and not the other components of the virus . Therefore , new virions or virus particles cannot be made or replicated . The simplest form of light , or optical , microscopes . Light , most often emitted from a standard halogen bulb , enters the microscope from the base bottom ) and is reflected via mirrors towards the sample . - ✔Bright Field Microscope Incoming light is in the form of the ultraviolet ( UV ) spectrum . - ✔Fluorescence Microscope Can provide detailed images of live cells without staining . - ✔Phase Contrast Microscope Use of beams of electrons ( rather than light ) to increase its resolution capacity to less than 1nm -that's 200x . - ✔Electron Microscope Combines the usefulness of fluorescence microscopy with the ability to visualize cells in 3 - D - ✔Confocal Microscope
This microscope reflects light off of the specimen at an angle rather than light passing through the specimen . - ✔Dark field Microscope Which of the following reactions is an example of a hydrolase ? - ✔A - B + H2O → A OH + B - H Which region in a four phase dilutional streak contains the highest concentration of a microbial organism ? Describe the technique that is used in pulling the sample from one phase to the next - ✔The first phase of the quadrant streak contains the highest concentration of a microbial organism . Using a new sterile loop , part of the sample is pulled from region 1 to region 2. This process is repeated again each time using a new sterile loop through the remaining regions . Identify the following disease based upon the physical manifestation : - ✔Anthrax Which of the following is / are true of Animalia ? ( Select all that apply ) - ✔They possess the ability to move; Humans are an example Each of the following statements about measles , mumps , and rubella is true , except . - ✔Infect the CNS ( central nervous system ) , leading to temporary or permanent paralysis There has been an outbreak of Staph Aureus within a local cleaning business . The employer Is trying to determine which of two companies has not been using sterile precautions when cleaning . To help determine who is responsible for unsanitary practices , a researcher streaks the isolated bacterial samples from each company onto MSA agar . After incubating the samples overnight at 37 ° C . , briefly explain how he will interpret the result of his samples . - ✔A sample showing only colorless colonies growing on red agar would indicate the presence of non - pathogenic bacteria , while a sample showing a distinct yellow agar would indicate the presence of Staph aureus . True or False The metabolism of a lipid - derived carbon atom will yield more ATP than a molecule of glucose - ✔True Giemsa staining is used to detect each of the following , except : - ✔Neisseria Gonorrhea What STD is caused by Gram - negative , diplococci , fastidious bacteria that may result in cardiac and neurological complications ? - ✔Gonorrhea