Catalogo Spring Bioscience

Page 1

Research Catalog

TARGETED PRODUCTS FOR CANCER RESEARCH

Primary Antibodies Detection Systems Antibody Microarrays Recombinant Proteins Other Complementary Reagents

2012


TECHNICAL CAPABILITIES

Recombinant Antibodies Mutation-Specific Antibodies Rabbit And Other Species Monoclonals


Ki-67 (SP6) Rabbit Monoclonal Human Tonsil

iNOS (SP126) Rabbit Monoclonal Human Lung

Actin-beta (SP124) Rabbit Monoclonal Human Placenta

F4/80 (SP115) Rabbit Monoclonal Mouse Liver

IKAROS (SP108)

FEATURING SP CLONES

Delivering Best-in-Class Rabbit Monoclonals since 2001.

Rabbit Monoclonal Human Tonsil

CD278/ICOS (SP98) Rabbit Monoclonal Human Reactive Lymph Node

FOXA1 (SP88) Rabbit Monoclonal Human Prostate


MESSAGE FROM SPRING BIOSCIENCE

Community of Cancer Researchers, Thank you for using our 2012 product catalog. Spring Bioscience is driven to build essential tools for cancer researchers so that tomorrow’s patients may benefit from today’s innovations. As a part of the Roche Diagnostics team and members of a society afflicted by cancer, we put tomorrow’s cancer patients at the center of our mission and are impassioned to enable researchers with high-quality tools for the analysis of cancer tissue. Spring was founded in 2001 by a team of scientists and achieved early success with their line of SP Clone rabbit monoclonal antibodies for clinical immunohistochemistry. This momentum was quickly noticed by Ventana Medical Systems who acquired the company in 2007 for its antibody development capabilities. Ventana Medical Systems was shortly thereafter acquired by Roche. Today, Spring serves an important role as an antibody center of excellence in Roche’s mission to deliver companion diagnostics. This is accomplished through not only developing antibodies destined for clinical assays through Ventana and other Roche affiliates but using our collective medical/market intelligence to serve the cancer research community. Our 2012 catalog highlights the dozens of new products we launched during 2011 and various other product updates including improved product utility and application images. By the time you are reading this catalog we will have also launched a new website at www. springbio.com which will contain a host of other product resources not available in the catalog. For example, hundreds of additional product images, customer feedback section, and much more. Thank you for the opportunity to assist you in your important and purposed work and if there is anything we can do to better serve you please let us know.

Sincerely,

Steve Reichling, General Manager, Spring Bioscience


TA B L E O F C O N T E N T S

Table of Contents Primary Antibody Products

11

Detection and Ancillary Products

109

Antibody Microarray Products

113

Recombinant Protein Products

115

Product Index

117


6

Urinary Bladder

Thyroid

Testis

Stomach

Soft Tissue

SM Intestine

Skin

Prostate

Liver

Pleura

Kidney

Placenta

Hematopoietic Cells

SP8

Pancreas

Endocrine

ALK/p80

Ovary

Colorectum

SP124

M308

Product Name

Osseous Tissue

Cervix

Actin-beta

Nervous Tissue

Cardiovascular

M424

Root Cat#

Lung

Clone

Breast

Rabbit Monoclonal Antibodies by Tissue Focus

M407

Androgen Receptor (AR)

SP107

M347

BAX

SP47

√ √

M366

BCL-2 alpha

SP66

M392

BOB-1

SP92

M391

C4d

SP91

M364

Cadherin-E/E-Cadherin

SP64

EC √

DC √

M390

Cadherin-N/N-Cadherin

SP90

M317

Calcitonin

SP17

M381

Calpain

SP81

M382

Calpastatin

SP82

M313

Calretinin

SP13

M406

Carbonic Anhydrase 9 (CA-9)

SP106

M343

Caveolin-1

SP43

M307

CD3

SP7

TC

M335

CD4

SP35

TC

M319

CD5

SP19

TC

M394

CD7

SP94

TC

M316

CD8

SP16

TC

M367

CD10

SP67

M410

CD19

SP110

BC

M332

CD20

SP32

BC

M404

CD22

SP104

BC

M323

CD23

SP23

M338

CD31

SP38

M355

CD43

SP55

M337

CD44 Std

SP37

M318

CD79a

SP18

M419

CD99

SP119

M326

CD117/c-kit

SP26

M396

CD163

SP96

M398

CD278/ICOS

SP98

M380

CDK2

SP80

M354

CDX2

SP54

√ √

BC EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

EC

√ √

EC

EC

TC √

BC √ √

TC

√ √

√ √

√ √

M303

c-erbB-2/HER-2

SP3

c-erbB-3/HER-3

SP71

M312

Chromogranin A

SP12

NE

NE

M344

c-Met

SP44

M359

c-Met (Paired 1003)

SP59

M358

c-Met (Phospho T1003)

SP58

M321

COX-2

SP21

M383

c-TEN

SP83

M304

Cyclin D1

SP4

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

M331

DOG-1

SP31

M309

Epidermal Growth Factor Receptor (EGFR)

SP9

M384

Epidermal Growth Factor Receptor (EGFR)

SP84

M368

ERCC1

SP68

M301

Estrogen Receptor (ER)

SP1

NE

√ √

NE

NE

NE

PLC

PLC

PLC

PLC

PLC

PLC

PLC

NE

√ NE

NE

M371

√ √

√ √

√ √

√ √

PLC

PLC

PLC

M415

F4/80

SP115

K

M

M

M

M423

FLK-1 (mouse)

SP123

M √

M √

M √

M388

FOXA1

SP88


SP86

hENT1

SP120

M408

IKAROS

SP108

M405

J-chain

SP105

M327

Keratin 5

SP27

M387

Keratin 6

SP87

M352

Keratin 7

SP52

M402

Keratin 8

SP102

Urinary Bladder

Thyroid

Prostate

Ovary

Testis

Pancreas

Osseous Tissue

√ √

Stomach

Glypican-3

M420

Soft Tissue

M386

SM Intestine

Skin

SP78

Pleura

Glial Fibrillary Acidic Protein (GFAP)

Placenta

SP97

M378

Nervous Tissue

FOXP3

Lung

M397

Liver

Kidney

Hematopoietic Cells

Endocrine

Colorectum

Cervix

Clone

Cardiovascular

Product Name

Breast

Root Cat#

PC

M399

Keratin 10

SP99

M353

Keratin 14

SP53

M395

Keratin 17

SP95

M369

Keratin 18

SP69

M333

Keratin 20

SP33

M306

Ki-67

SP6

M351

LMO2

SP51

M385

MCM2

M374

PLC

PLC

PLC

PLC

PLC

PLC

SP85

Mesothelin

SP74

M346

MSH2

SP46

M393

MSH6

SP93

M414

MUM1/IRF4

SP114

M372

Myeloperoxidase (MPO)

SP72

M403

Nestin

SP103

ST

M426

Nitric Oxide SynthaseInducible (iNOS)

SP126

M363

p120 (catenin delta 1)

SP63

M305

p53

SP5

√ √

√ √

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

PLC

M

M

√ √

M √

ST

M √

M √

ST M √

M418

p57

SP118

M334

PAX-5

SP34

M362

PI3K p85

SP62

M315

PLAP

SP15

M302

Progesterone Receptor (PR)

SP2

M342

Progesterone Receptor (PR)

SP42

M329

PSMA

SP29

M350

S6 Ribosomal (Phospho S235/236)

SP50

M345

S6 Ribosomal (Phospho S240/244)

SP45

ST

ST

ST

√ √

√ √

√ √

√ √

M422

SLIT2

SP122

M376

SOX-2

SP76

ST

M349

Stathmin

SP49

M379

Survivin

SP79

M311

Synaptophysin

SP11

NE

NE

NE

NE

M336

Syntaxin 1A

SP36

M370

Tau (Neuronal Marker)

SP70

M412

Thymidylate Synthase (TS)

SP112

M373

Uroplakin III

SP73

M328

Vascular Endothelial Growth Factor (VEGF)

SP28

M320

Vimentin

SP20

M417

Vinculin

SP117

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

MC, EC

ST

ST

ST

NE

ST

NE

√ √

√ √

ST √

NE

√ √

NE

√ √

ST

√ √

√ √

√ Indicates the organs or tumors derived from these organs express these markers using the information available in the public domain. Abbreviation: TC = T lymphocyte, BC = B lymphocyte, DC=dendritic cell, EC =endothelial cell, PC= plasma cell, NE = neuroendocrine cell, PLC =proliferating cell, ST=stem cell, M=macrophages, K=Kupffer cells, MC=Cardiac, skeletal, and smooth cells.

7


8

SP124

M308

ALK/p80

SP8

M407

Androgen Receptor (AR)

SP107

BAX

SP47

BCL-2 alpha

SP66

BOB-1

SP92

C4d

SP91

√ √

M364

Cadherin-E/E-Cadherin

SP64

M390

Cadherin-N/N-Cadherin

SP90

M317

Calcitonin

SP17

M381

Calpain

SP81

M382

Calpastatin

SP82

M313

Calretinin

SP13

M406

Carbonic Anhydrase 9 (CA-9)

SP106

√ √ √ √ √

M343

Caveolin-1

SP43

M307

CD3

SP7

M335

CD4

SP35

M319

CD5

SP19

M394

CD7

SP94

M316

CD8

SP16

M367

CD10

SP67

M410

CD19

SP110

M332

CD20

SP32

M404

CD22

SP104

M323

CD23

SP23

M338

CD31

SP38

M355

CD43

SP55

M337

CD44 Std

SP37

M318

CD79a

SP18

M419

CD99

SP119

M326

CD117/c-kit

SP26

M396

CD163

SP96

M398

CD278/ICOS

SP98

M380

CDK2

SP80

√ √ √

√ √ √ √ √ √ √ √ √ √

M354

CDX2

SP54

M303

c-erbB-2/HER-2

SP3

M371

c-erbB-3/HER-3

SP71

M312

Chromogranin A

SP12

M344

c-Met

SP44

M359

c-Met (Paired 1003)

SP59

M358

c-Met (phospho T1003)

SP58

M321

COX-2

SP21

M383

c-TEN

SP83

√ √

M304

Cyclin D1

SP4

M331

DOG-1

SP31

M309

Epidermal Growth Factor Receptor (EGFR)

SP9

M384

Epidermal Growth Factor Receptor (EGFR)

SP84

M368

ERCC1

SP68

M301

Estrogen Receptor (ER)

SP1

M415

F4/80

SP115

√ √

√ √ √

Translational Control

Transcriptional Regulators

Synthesis and Degradation

Steroid Receptors

Stem Cell

Signal Transduction

Oncogenesis

Nitrogen Metabolism

Neuroscience

Lymphocyte Signaling

Immunoglobulin

Hormones

Homeodomain Proteins

Hematopoiesis

Enzymes

EGFR Family

DNA Synthesis

DNA Replication and Repair

Cytoskeletal Proteins

Complement

Cell Junctions

Cell Cycle

Cell Adhesion

√ √

M347 M392

Cancer Markers

M366 M391

B Cell Markers

Clone

Actin-beta

Apoptosis

Product Name

M424

Angiogenesis

Root Cat#

AKT/PKB Signaling

Rabbit Monoclonal Antibodies by Major Research Interest


SP123

M388

FOXA1

SP88

M397

FOXP3

SP97

M378

Glial Fibrillary Acidic Protein (GFAP)

SP78

M386

Glypican-3

SP86

M420

hENT1

SP120

M408

IKAROS

SP108

M405

J-chain

SP105

M327

Keratin 5

SP27

M387

Keratin 6

SP87 √ √

M395

Keratin 17

SP95

Translational Control

Transcriptional Regulators

Synthesis and Degradation

Steroid Receptors

Stem Cell

Signal Transduction

Oncogenesis

Nitrogen Metabolism

Neuroscience

Lymphocyte Signaling

Immunoglobulin

Hormones

√ √

SP52 SP99

Homeodomain Proteins

√ √

SP102 SP53

Hematopoiesis

√ √

Keratin 7 Keratin 10

Enzymes

√ √

Keratin 8 Keratin 14

EGFR Family

M352 M399

DNA Synthesis

M402 M353

DNA Replication and Repair

Cytoskeletal Proteins

Complement

Cell Junctions

Cell Cycle

Cell Adhesion

Cancer Markers

B Cell Markers

Apoptosis

Clone

FLK-1 (mouse)

Angiogenesis

Product Name

M423

AKT/PKB Signaling

Root Cat#

√ √

M369

Keratin 18

SP69

M333

Keratin 20

SP33

M306

Ki-67

SP6

M351

LMO2

SP51

M385

MCM2

SP85

√ √ √

M374

Mesothelin

SP74

M346

MSH2

SP46

√ √

M393

MSH6

SP93

M414

MUM1/IRF4

SP114

M372

Myeloperoxidase (MPO)

SP72

√ √

M403

Nestin

SP103

M426

Nitric Oxide SynthaseInducible (iNOS)

SP126

M363

p120 (catenin delta 1)

SP63

M305

p53

SP5

M418

p57

SP118

M334

PAX-5

SP34

M362

PI3k p85

SP62

M315

PLAP

SP15

M302

Progesterone Receptor (PR)

SP2

M342

Progesterone Receptor (PR)

SP42

M329

PSMA

SP29

M350

S6 Ribosomal (phospho S235/236)

SP50

M345

S6 Ribosomal (phospho S240/244)

SP45

M422

SLIT2

SP122

M376

SOX-2

SP76

M349

Stathmin

SP49

M379

Survivin

SP79

M311

Synaptophysin

SP11

M336

Syntaxin 1A

SP36

M370

Tau (Neuronal Marker)

SP70

M412

Thymidylate Synthase (TS)

SP112

M373

Uroplakin III

SP73

M328

Vascular Endothelial Growth Factor (VEGF)

SP28

M320

Vimentin

SP20

M417

Vinculin

SP117

√ √ √

√ √

√ √

√ √ √ √ √ √ √ √ √ √ √ √

9


FEATURING SP CLONES

Rabbit Monoclonal

Delivering Best-in-Class Rabbit Monoclonals since 2001.

CD19

Rabbit Monoclonal

SP = Spring Bioscience Corporation While you may not be familiar with Spring Bioscience Corporation, you are probably quite familiar with rabbit monoclonal “SP� clones. All SP antibody clone products were CD19 Rabbit Monoclonal designed and manufactured by Spring Bioscience. It all started with SP1, the now gold standard Estrogen Receptor marker. SP clones continue to emerge as best-in-class immunohistochemistry markers for their high affinity, sensitivity, and specificity.

10

Terms and Conditions Spring Bioscience products are for research use only. They are not for use in diagnostic procedures. Spring Bioscience strives to provide complete and accurate information; however, Spring Bioscience and its business associates assume no liability for any reliance on any of the information provided. Furthermore, Spring Bioscience assumes no liability for any omissions. It is the sole responsibility of researchers to independently validate the information and proper use of any products. Spring Bioscience reserves the right to make any changes, additions, and/or deletions to its product information at anytime without notice.


PRIMARY ANTIBODY PRODUCTS

Spring Bioscience develops and manufactures a wide range of products primarily specialized for IHC. Primary Antibodies – specifically characterized for use in IHC, featuring: • Clean and intense staining • Lower pre-treatment requirement • Robust performance for FFPE tissue • Improved reproducibility • Accepted by industry leaders Available in 0.1ml, 0.5ml and 1.0ml concentrated sizes as well as a 7ml ready-to-use configuration. Antibody product technologies include: • Rabbit Monoclonal (SP clones) • Mouse Monoclonal (SPM clones) • Rabbit Polyclonal

11


14-3-3 beta Rabbit Polyclonal

Actin-beta Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E4550

0.1ml

Concentrate

Immunogen: N-terminus of human beta actin.

Concentrate

Immunogen: Synthetic peptide from N-terminus of human 14-3-3 beta.

E4552

0.5ml

Concentrate

Concentrate

Application: IHC-P, WB

Application: IHC-P

1.0ml

E4554

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E4551

7.0ml

Ready-to-use

Species Reactivity: Human*

Catalog No.

Size

Format

E2920

0.1ml

Concentrate

E2922

0.5ml

E2924 E2921

Isotype: Rabbit IgG

Clone: N/A

Isotype: Rabbit IgG Control Tissue: Leiomyoma, Skin

Control Tissue: Brain, Prostate Carcinoma, Liver Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm Control Lysate: Jurkat Molecular Weight: 30 kDa Human Leiomyoma stained with anti-Actin-beta antibody E455

Human Brain stained with anti-14-3-3 beta antibody E292

Product Description: At least 7 mammalian isoforms comprise the 14-3-3 family of signaling intermediates. 14-3-3 molecules are highly conserved, expressed in a broad range of tissues and cell types and were first discovered as a series of highly abundant proteins in brain tissue. There is increasing evidence that 14-3-3 proteins are critical to cell transformation and mitotic signaling.

Product Description: The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Antibody to β-actin provides a specific and useful tool in studying the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.

14-3-3 zeta Rabbit Polyclonal

Actin-Skeletal Muscle Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3880

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human 14-3-3 zeta.

E3882

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P, WB

E3884

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E3881

7.0ml

Ready-to-use

Catalog No.

Size

Format

E12600

0.1ml

Concentrate

E12602

0.5ml

E12604 E12601

Clone: N/A Immunogen: Synthetic peptide derived from N-terminus of human alpha skeletal muscle isoform of actin. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Tonsil

Control Tissue: Skeletal Muscle

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Control Lysate: 293T Molecular Weight: 28 kDa

Human Skeletal Muscle stained with anti-Actin-Skeletal Muscle antibody E388

Human Breast Carcinoma stained with anti-14-3-3 zeta antibody E1260

Product Description: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation and activation/repression of enzyme activity.

Product Description: This antibody reacts with sarcomeric actins of normal tissues and neoplasms derived from such tissues (i.e. rhabdomyosarcomas).

Actin-beta Rabbit Monoclonal

Actin-Skeletal Muscle Mouse Monoclonal Clone: 5C5.F8.C7

Clone: SP124

Catalog No.

Size

Format

E16650

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide near the N-terminus of human actin-beta protein.

E16652

0.5ml

Concentrate

Immunogen: N-terminus decapeptide of alpha skeletal muscle isoform of actin.

1.0ml

Concentrate

Application: IHC-P, WB

E16654

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E16651

7.0ml

Ready-to-use

Catalog No.

Size

Format

M4240

0.1ml

Concentrate

M4242

0.5ml

M4244 M4241

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgM

Control Tissue: Placenta

Control Tissue: Skeletal Muscle

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Control Lysate: Raji Molecular Weight: 42 kDa Human Placenta stained with anti-Actin-beta antibody M424

Product Description: Beta-actin is a nonmuscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

12

Human Skeletal Muscle stained with anti-Actin-Skeletal Muscle antibody E1665

Product Description: This antibody reacts with sarcomeric actins of normal tissues and neoplasms derived from such tissues (i.e. rhabdomyosarcomas).

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Actin-Smooth Muscle Rabbit Polyclonal

Adrenocorticotrophic Hormone (ACTH) Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E3490

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin.

E3492

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human ACTH.

1.0ml

Concentrate

Application: IHC-P

E3494

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E3491

7.0ml

Ready-to-use

Catalog No.

Size

Format

E2460

0.1ml

Concentrate

E2462

0.5ml

E2464 E2461

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Leiomyoma, Smooth Muscle in Small Intestine

Control Tissue: Pituitary Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Smooth Muscle in Small Intestine stained with anti-Actin-Smooth Muscle antibody E246

Human Pituitary stained with anti-Adrenocorticotrophic Hormone (ACTH) antibody E349

Product Description: This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. Actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), and myocardium (alpha-myocardial) are not stained.

Product Description: ACTH labels corticotrophs in the adenohypophysis and is useful in the classification of pituitary adenomas.

Actin-Smooth Muscle Mouse Monoclonal

Adrenocorticotrophic Hormone (ACTH) Mouse Monoclonal

Clone: 1A4

Catalog No.

Size

Format

E16450

0.1ml

Concentrate

Immunogen: ACTH synthetic peptide.

Concentrate

Immunogen: Synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin.

E16452

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P

E16454

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E16451

7.0ml

Ready-to-use

Species Reactivity: Human*

Catalog No.

Size

Format

E14340

0.1ml

Concentrate

E14342

0.5ml

E14344 E14341

Isotype: Mouse IgG1/k

Clone: SPM501

Isotype: Mouse IgG1 Control Tissue: Pituitary

Control Tissue: Leiomyoma, Smooth Muscle

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Smooth Muscle stained with anti-Actin-Smooth Muscle antibody E1434

Human Pituitary stained with anti-Adrenocorticotrophic Hormone (ACTH) antibody E1645

Product Description: This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. Actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), and myocardium (alpha-myocardial) are not stained.

Product Description: ACTH labels corticotrophs in the adenohypophysis and is useful in the classification of pituitary adenomas.

Adenomatous Polyposis Coli (APC) Rabbit Polyclonal

AKT-1/AKT-2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E10840

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human APC protein.

E10842

0.5ml

Concentrate

Immunogen: Synthetic peptide from N-terminus of human AKT-1.

1.0ml

Concentrate

Application: IHC-P

E10844

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E10841

7.0ml

Ready-to-use

Catalog No.

Size

Format

E4080

0.1ml

Concentrate

E4082

0.5ml

E4084 E4081

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Colon Carcinoma

Control Tissue: Lung Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Colon Carcinoma stained with anti-Adenomatous Polyposis Coli (APC) antibody E408

Human Lung Carcinoma stained with anti-AKT-1/AKT-2 antibody E1084

Product Description: APC is associated with structural components of intracellular junctions, including conductin, and it competes with E-cadherin for binding to specific internal regions of both β- and γ-catenin. Like APC, APC2 contains SAMP domains, which are required for conductin binding. Both APC and APC2 regulate the formation of active β-catenin-Tcf complexes.

Product Description: Two serine/threonine kinases, designated AKT-1 and AKT-2 (alternatively referred to as PKB or RacPK), exhibit sequence homology with the protein kinase A and C families and are encoded by the c-AKT proto-oncogene. AKT-1 and AKT-2 contain a Pleckstrin homology (PH) domain and are activated by PDGF stimulation. This activation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the 85kDa subunit of the phosphatidylinositol 3-kinase (PI-3 kinase) complex.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

13


AKT-1/PKB (Phospho S473) Rabbit Polyclonal Catalog No.

Size

Format

E13450

0.1ml

Concentrate

E13452

0.5ml

Concentrate

E13454

1.0ml

Concentrate

E13451

7.0ml

Ready-to-use

alpha Fetoprotein (AFP) Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide derived from the region of human AKT-1 that contains phosphorylated site of serine 473.

E2950

0.1ml

Concentrate

E2952

0.5ml

Concentrate

Immunogen: Human alpha-fetoprotein purified from human cord serum.

E2954

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P, WB

E2951

7.0ml

Ready-to-use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG Control Tissue: Breast Carcinoma

Control Tissue: Human alpha-fetoprotein purified from human cord serum.

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm Control Lysate: HepG2 Molecular Weight: 70 kDa

Human Breast Carcinoma stained with anti-AKT-1/PKB (Phospho S473) antibody E1345

Human Fetal Liver stained with anti-alpha Fetoprotein (AFP) antibody E295

Product Description: The AKT-1 serine threonine protein kinase is catalytically inactive in serum starved primary and immortalized fibroblasts and activated by platelet derived growth factor. The activation is rapid and specific. Survival factors can suppress apoptosis in a transcription independent manner by activating AKT-1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody is specific to the phosphorylated form of AKT-1.

Product Description: AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been identified in hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

ALK/p80 Rabbit Monoclonal

alpha-1-Antichymotrypsin Rabbit Polyclonal

Catalog No.

Size

Format

M3080

0.1ml

Concentrate

M3082

0.5ml

Concentrate

M3084

1.0ml

Concentrate

M3081

7.0ml

Ready-to-use

Clone: SP8 Immunogen: Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript. Application: IHC-P Species Reactivity: Human*

Clone: N/A

Catalog No.

Size

Format

E3450

0.1ml

Concentrate

Immunogen: Purified human alpha-1-antichymotrypsin.

E3452

0.5ml

Concentrate

Application: IHC-P

E3454

1.0ml

Concentrate

E3451

7.0ml

Ready-to-use

Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Tonsil

Isotype: Rabbit IgG

Cellular Localization: Cytoplasm

Control Tissue: Anaplastic Lymphoma Cellular Localization: Cytoplasm, Nucleus

Human Anaplastic Lymphoma stained with anti-ALK/p80 antibody M308

Human Tonsil stained with anti-alpha-1-Antichymotrypsin antibody E345

Product Description: This antibody recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This antibody can be used to detect p80 in these lymphomas and may also be used to detect a subtype of large B cell lymphoma, which expresses the full-length ALK protein.

Product Description: Alpha-1-Antichymotrypsin (Alpha 1-ACT) is an early-stage acute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin, cathepsin G, and chymase. Alpha-1-ACT, a serine protease inhibitor, is tightly associated with amyloid plaques in Alzheimer’s disease (AD) and in normal aged human and monkey brain. Regulation of the serine proteases and serine protease inhibitors plays an important role in neuromuscular differentiation.

ALK/p80 Rabbit Polyclonal

alpha-1-Antitrypsin Rabbit Polyclonal

Catalog No.

Size

Format

E1090

0.1ml

Concentrate

E1092

0.5ml

Concentrate

E1094

1.0ml

Concentrate

E1091

7.0ml

Ready-to-use

Clone: N/A Immunogen: Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C- terminus of the NPM-ALK transcript. Application: IHC-P Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Anaplastic Lymphoma

Clone: 5C5.F8.C7

Catalog No.

Size

Format

E3440

0.1ml

Concentrate

Immunogen: Purified human alpha-1-antitrypsin.

E3442

0.5ml

Concentrate

Application: IHC-P

E3444

1.0ml

Concentrate

E3441

7.0ml

Ready-to-use

Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Tonsil Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Nucleus

Human Anaplastic Lymphoma stained with anti-ALK/p80 antibody E109

Product Description: This antibody recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This antibody can be used to detect p80 in these lymphomas and may also be used to detect a subtype of large B cell lymphoma, which expresses the full-length ALK protein.

14

Human Tonsil stained with anti-alpha-1-Antitrypsin antibody E344

Product Description: Alpha-1-antitrypsin (alpha-1-AT) exists in a number of genetic variants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema).

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Amylin Peptide Rabbit Polyclonal

Androgen Receptor (AR) Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

M4070

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human amylin protein.

M4072

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human AR.

1.0ml

Concentrate

Application: IHC-P

M4074

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M4071

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3400

0.1ml

Concentrate

E3402

0.5ml

E3404 E3401

Clone: SP107

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas

Control Tissue: Prostate Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Pancreas stained with anti-Amylin Peptide antibody E340

Human Prostate Carcinoma stained with anti-Androgen Receptor (AR) antibody M407

Product Description: Amylin is a pancreatic islet peptide with a role in the maintenance of glucose homoeostasis. It is predominantly found in the beta cells of the pancreas and to a lesser extent in the gastrointestinal tract and nervous system. This antibody may prove of value in studies to determine the level of staining for amylin in normal and diabetic pancreas and pancreatic carcinoma.

Product Description: The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. The expression of AR is reportedly inversely correlated with histologic grade, i.e., well differentiated prostate tumors show higher expression than the poorly differentiated tumors.

Amyloid Precursor Protein (APP) Rabbit Polyclonal

Androgen Receptor (AR) Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E2720

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human APP.

E2722

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human AR.

1.0ml

Concentrate

Application: IHC-P

E2724

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E2721

7.0ml

Ready-to-use

Catalog No.

Size

Format

E2650

0.1ml

Concentrate

E2652

0.5ml

E2654 E2651

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Alzheimer’s Brain

Control Tissue: Prostate Carcinoma

Cellular Localization: Cytoplasm, Extracellular Matrix

Cellular Localization: Nucleus Control Lysate: LnCap Molecular Weight: 110 kDa

Human Alzheimer’s Brain stained with anti-Amyloid Precursor Protein (APP) antibody E265

Human Prostate Carcinoma stained with anti-Androgen Receptor (AR) antibody E272

Product Description: This antibody recognizes APP (β-APP770), MW 110kDa. Predicted to recognize β-APP695 and β-APP751. Amyloid precursor protein (APP) and APP-like proteins are transmembrane glycoproteins with a similar modular domain structure.

Product Description: The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. The expression of AR is reportedly inversely correlated with histologic grade, i.e., well differentiated prostate tumors show higher expression than the poorly differentiated tumors.

Amyloid-beta/Amyloid A4 Rabbit Polyclonal

Annexin I Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E14030

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from N-terminus of human amyloid beta.

E14032

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human annexin I.

1.0ml

Concentrate

Application: IHC-P

E14034

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E14031

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3530

0.1ml

Concentrate

E3532

0.5ml

E3534 E3531

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Alzheimer’s Brain

Control Tissue: Placenta

Cellular Localization: Transmembrane, Extracellular Matrix

Cellular Localization: Membrane

Human Alzheimer’s Brain stained with anti-Amyloid-beta/Amyloid A4 antibody E353

Human Placenta stained with anti-Annexin I antibody E1403

Product Description: Amyloid beta-protein/amyloid A4 or amyloid beta-peptide is derived from the amyloid betaprecursor protein. Beta amyloid is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles in Alzheimer’s brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, amyloidosis (Dutch type) and in the Guam Parkinson-Dementia complex.

Product Description: Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis and cellular adhesion. Annexin A1, a gene related to phagocytosis, was found to be one of the most highly upregulated genes in hairy cell leukemia.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

15


Annexin VI Rabbit Polyclonal

BAX Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3470

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human Annexin VI.

M3472

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human BAX protein.

1.0ml

Concentrate

Application: IHC-P, WB

M3474

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3471

7.0ml

Ready-to-use

Catalog No.

Size

Format

E12380

0.1ml

Concentrate

E12382

0.5ml

E12384 E12381

Clone: SP47

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Liver, Tonsil

Control Tissue: Breast, Colon

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane

Control Lysate: 293T Molecular Weight: 75 kDa Human Liver stained with anti-Annexin VI antibody E1238

Human Breast stained with anti-BAX antibody M347

Product Description: Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis and cellular adhesion. Annexin VI is positively regulated by calcium. It contains 8 annexin repeats and it binds actin filament and lipids. Annexin VI has been localized to stress fibers, membrane ruffles, and microspikes.

Product Description: BAX is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX can form homodimers and is also able to heterodimerize with other BCL-2 related proteins.

Apoptosis Protease Activating Factor 1 (APAF-1) Rabbit Polyclonal

BAX Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E17990

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human APAF-1.

E17992

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human BAX protein.

1.0ml

Concentrate

Application: IHC-P

E17994

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E17991

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3950

0.1ml

Concentrate

E3952

0.5ml

E3954 E3951

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Testis

Control Tissue: Breast, Colon

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Testis stained with anti-Apoptosis Protease Activating Factor 1 (APAF-1) antibody E395

Human Breast stained with anti-BAX antibody E1799

Product Description: APAF-1 is a cytosolic protein that rests in a latent state until bound by cytochrome c (APAF-2). The resulting APAF-1/cytochrome c complex associates with the zymogen form of caspase-9 (APAF-3) in the presence of dATP or ATP, promoting the autocatalytic activation of caspase-9. This antibody can be useful in studies of apoptotic cell death.

Product Description: BAX is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX can form homodimers and is also able to heterodimerize with other BCL-2 related proteins.

BAK Rabbit Polyclonal

BCL-2 alpha** Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3660

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human BAK.

M3662

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human BCL-2 alpha protein.

1.0ml

Concentrate

Application: IHC-P

M3664

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3661

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3320

0.1ml

Concentrate

E3322

0.5ml

E3324 E3321

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Tonsil stained with anti-BAK antibody E332

Human Tonsil stained with anti-BCL-2 alpha antibody M366

Product Description: BAK or BCL-2-homologous antagonist is a member of the BCL-2 family of homologous proteins which function in the regulation of apoptosis. The BCL-2 related proteins interact with one another through the formation of homo- and heterodimers. BAK has been localized by immunohistochemistry to the cytosol of cells and stains in a punctate pattern characteristic of other BCL-2 family proteins, which have been localized to intracellular organelles.

16

Clone: SP66

Product Description: Expression of BCL-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies BCL-2 alpha Rabbit Polyclonal

BCL-10 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E19030

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human BCL-2 alpha protein.

E19032

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human BCL-10.

1.0ml

Concentrate

Application: IHC-P

E19034

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E19031

7.0ml

Ready-to-use

Catalog No.

Size

Format

E17980

0.1ml

Concentrate

E17982

0.5ml

E17984 E17981

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Human Tonsil stained with anti-BCL-2 alpha antibody E1798

Human Tonsil stained with anti-BCL-10 antibody E1903

Product Description: Expression of BCL-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.

Product Description: BCL-10/CIPER/CLAP/mE10 is expressed in many human and murine tissues. It contains a CARD (caspase recruiting domain) domain and is involved in activation of caspase 9 to induce apoptosis. It is a cellular homolog of equine herpesvirus 2 E-10. It is known to induce activation of JNK, p38 and NF-kappaB. Mutations in BCL-10 are observed in many B and T cell lymphomas implicating its role in pathogenesis of human cancer.

BCL-6 Rabbit Polyclonal

BCL-10 Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E16760

0.1ml

Concentrate

Immunogen: Recombinant BCL-10 protein.

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human BCL-6 protein.

E16762

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P

1.0ml

E16764

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E16761

7.0ml

Ready-to-use

Species Reactivity: Human*

Catalog No.

Size

Format

E14090

0.1ml

Concentrate

E14092

0.5ml

E14094 E14091

Isotype: Rabbit IgG

Clone: SPM520

Isotype: Mouse IgG1 Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Nucleus

Human Tonsil stained with anti-BCL-6 antibody E1409

Human Tonsil stained with anti-BCL-10 antibody E1676

Product Description: BCL-6 proto-oncogene product is mainly expressed in normal germinal center B cells and related lymphomas. BCL-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas and BCL-6 rearrangements have been detected in diffuse large B cell lymphomas. BCL-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt lymphomas and in nodular, lymphocyte predominant Hodgkin’s disease.

Product Description: BCL-10/CIPER/CLAP/mE10 is expressed in many human and murine tissues. It contains a CARD (caspase recruiting domain) domain and is involved in activation of caspase 9 to induce apoptosis. It is a cellular homolog of equine herpesvirus 2 E-10. It is known to induce activation of JNK, p38 and NF-kappaB. Mutations in BCL-10 are observed in many B and T cell lymphomas implicating its role in pathogenesis of human cancer.

BCL-10 Rabbit Polyclonal

BCL-X Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3370

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide near the N-terminus of human BCL-10 protein.

E3372

0.5ml

Concentrate

Immunogen: Synthetic protein derived from near N-terminus of the human BCL-X protein.

1.0ml

Concentrate

Application: IHC-P

E3374

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E3371

7.0ml

Ready-to-use

Catalog No.

Size

Format

E19240

0.1ml

Concentrate

E19242

0.5ml

E19244 E19241

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Hodgkin’s Lymphoma

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm, Membrane, Nucleus Control Lysate: 293T Molecular Weight: 27 kDa

Human Tonsil stained with anti-BCL-10 antibody E1924

Product Description: BCL-10 is a cytoplasmic protein expressed at different levels in most normal human cells and translocates into the nucleus of MALT lymphoma, especially in those with translocation (1;14)(p22;q32). BCL-10 and MALT-1 protein form a complex in MALT lymphoma. They are thought to synergize in the activation of NF-kappaB, and may contribute to the same pathogenic process that leads to malignancy. Mutations in BCL-10 are observed in some B and T cell lymphomas.

Human Hodgkin’s Lymphoma stained with anti-BCL-X antibody E337

Product Description: BCL-X is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. BCL-X has two isoforms, BCL-XL (long) a 241 amino acid protein and BCL-XS (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the BCL-2 family.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

17


BCL-XL Rabbit Polyclonal

Bnip3L Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

E16840

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human BCL-X protein.

E16842

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human BNIP3L protein.

1.0ml

Concentrate

Application: IHC-P, WB

E16844

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E16841

7.0ml

Ready-to-use

Catalog No.

Size

Format

E4200

0.1ml

Concentrate

E4202

0.5ml

E4204 E4201

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma, Hodgkin’s Lymphoma

Control Tissue: Breast Carcinoma Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane, Nucleus Control Lysate: 293T Molecular Weight: 27 kDa

Human Breast Carcinoma stained with anti-Bnip3L antibody E1684

Human Colon Carcinoma stained with anti-BCL-XL antibody E420

Product Description: BCL-X is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. BCL-X has two isoforms, BCL-XL (long) a 241 amino acid protein and BCL-XS (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the BCL-2 family.

Product Description: BCL2/adenovirus E1B 19kDa protein-interacting protein 3-like (Bnip3L) is a protein encoded by the BNIP3L gene in humans. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppression by BCL-2 and BCL-XL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

BCL-XL Mouse Monoclonal

BOB-1 Rabbit Monoclonal Clone: SP92

Clone: 7D9

Catalog No.

Size

Format

M3920

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of mouse BCL-XL protein.

M3922

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human BOB-1 protein.

1.0ml

Concentrate

Application: IHC-P

M3924

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

M3921

7.0ml

Ready-to-use

Catalog No.

Size

Format

E16660

0.1ml

Concentrate

E16662

0.5ml

E16664 E16661

Species Reactivity: Human*

Isotype: Mouse IgG2a

Isotype: Rabbit IgG

Control Tissue: Hodgkin’s Lymphoma

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane, Nucleus

Cellular Localization: Nucleus Control Lysate: Ramos Molecular Weight: 35 kDa Human Tonsil stained with anti-BOB-1 antibody M392

Human Hodgkin’s Lymphoma stained with anti-BCL-XL antibody E1666

Product Description: BCL-X is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. BCL-X has two isoforms, BCL-XL (long) a 241 amino acid protein and BCL-XS (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the BCL-2 family.

Product Description: B cell Oct binding protein 1 (BOB-1), is a transcription factor that is required for germinal center formation and immunoglobulin production. BOB-1 is expressed at high levels in spleen and peripheral blood leukocytes and represents an OCT co-factor capable of conferring cell specific activation of OCT-1 and OCT-2.

BIM/BOD Rabbit Polyclonal

BOB-1 Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

E16670

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human BIM.

E16672

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human BOB-1.

1.0ml

Concentrate

Application: IHC-P

E16674

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E16671

7.0ml

Ready-to-use

Catalog No.

Size

Format

E4360

0.1ml

Concentrate

E4362

0.5ml

E4364 E4361

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus Control Lysate: Ramos Molecular Weight: 35 kDa

Human Placenta stained with anti-BIM/BOD antibody E436

Human Tonsil stained with anti-BOB-1 antibody E1667

Product Description: BIM/BOD (BCL-2-related Ovarian Death Gene) belongs to BCL-2 family of proteins containing BCL-2 homology domain3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the BCL-2 family like BCL-2, BCL-XL and BCL-W. It exhibits three splice variants; BIML, BIMS and BIMEL. They all share homology in their C-terminus BH3 domain and are ubiquitously expressed. BIMS is the strongest of them with respect to the proapoptotic capacity.

Product Description: B cell Oct binding protein 1 (BOB-1), is a transcription factor that is required for germinal center formation and immunoglobulin production. BOB-1 is expressed at high levels in spleen and peripheral blood leukocytes and represents an OCT co-factor capable of conferring cell specific activation of OCT-1 and OCT-2.

18

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Bone Morphogenetic Protein 4 (BMP-4) Rabbit Polyclonal

c-Abl Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Recombinant human BMP-4 protein.

E4000

0.1ml

Concentrate

Concentrate

Application: IHC-P

E4002

0.5ml

Concentrate

1.0ml

Concentrate

1.0ml

Concentrate

Ready-to-use

Species Reactivity: Human*

E4004

7.0ml

E4001

7.0ml

Ready-to-use

Catalog No.

Size

Format

E12510

0.1ml

E12510

0.5ml

E12514 E12511

Isotype: Rabbit IgG

Clone: N/A Immunogen: Synthetic peptide from the sequence adjacent to protein tyrosine kinase domain of human c-Abl. Application: IHC-P Species Reactivity: Human*

Control Tissue: Bone Marrow

Isotype: Rabbit IgG

Cellular Localization: Cytoplasm

Control Tissue: Placenta Cellular Localization: Cytoplasm

Human Bone Marrow stained with anti-Bone Morphogenetic Protein 4 (BMP-4) antibody E1251

Human Placenta stained with anti-c-Abl antibody E400

Product Description: Bone morphogenetic protein 4 (BMP4) is one of nine structurally related BMPs belonging to the transforming growth factor beta superfamily of secreted proteins. BMP4 has important roles as a signaling molecule in the developing central and peripheral nervous system, musculature and skeleton. BMP also plays an important role in early tooth morphogenesis.

Product Description: The c-Abl proto-oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome). The molecular consequence of this translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase.

C4d** Rabbit Monoclonal

Cadherin-E/E-Cadherin** Rabbit Monoclonal Clone: SP91

Catalog No.

Size

Format

M3640

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human C4d protein.

M3642

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 600-707 of human cadherin-E.

1.0ml

Concentrate

Application: IHC-P

M3644

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

M3641

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3910

0.1ml

Concentrate

M3912

0.5ml

M3914 M3911

Clone: SP64

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Breast Carcinoma, Colon

Cellular Localization: Secreted Protein

Cellular Localization: Membrane Control Lysate: MCF7 Molecular Weight: 135 kDa

Human Tonsil stained with anti-C4d antibody M391

Human Breast Carcinoma stained with anti-Cadherin-E/E-Cadherin antibody M364

Product Description: C4 plays a central role in the activation of the classical pathway of the complement system. Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.

Product Description: Cadherin-E/E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity.

C4d Rabbit Polyclonal

Cadherin-E/E-Cadherin Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Synthetic peptide of human C4d.

E8640

0.1ml

Concentrate

Concentrate

Application: IHC-P

E8642

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 600-707 of human cadherin-E.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-use

Species Reactivity: Human*

E8644

7.0ml

E8641

7.0ml

Ready-to-use

Catalog No.

Size

Format

E17340

0.1ml

E17342

0.5ml

E17344 E17341

Isotype: Rabbit IgG

Clone: SPM471

Species Reactivity: Human* Isotype: Mouse IgG2b/k

Control Tissue: Kidney, Tonsil Cellular Localization: Secreted Protein

Control Tissue: Breast, Skin, Tonsil

Human Tonsil stained with anti-C4d antibody E1734

Human Breast stained with anti-Cadherin-E/E-Cadherin antibody E864

Product Description: C4 plays a central role in the activation of the classical pathway of the complement system. Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.

Cellular Localization: Membrane

Product Description: E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

19


Cadherin-E/E-Cadherin Rabbit Polyclonal

Cadherin-pan Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E2360

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 600-707 of human cadherin-E.

E2362

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of chicken cadherin-N protein.

1.0ml

Concentrate

Application: IHC-P

E2364

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E2361

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3460

0.1ml

Concentrate

E3462

0.5ml

E3464 E3461

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast, Skin, Tonsil

Control Tissue: Skin, Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Skin stained with anti-Cadherin-E/E-Cadherin antibody E346

Human Skin stained with anti-Cadherin-pan antibody E236

Product Description: E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity.

Product Description: Cadherins are calcium dependent cell adhesion molecules, which play important roles in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. In adhesion junctions, cadherins are bound to β and γ catenins, which in turn bind to α catenin, an actin-binding protein. Cadherins play important roles in tumor invasion and metastasis.

Cadherin-N/N-Cadherin Rabbit Monoclonal

Calbindin D28 Rabbit Polyclonal

Clone: SP90

Catalog No.

Size

Format

E10340

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cadherin-N protein.

E10342

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human calbindin D28K protein.

1.0ml

Concentrate

Application: IHC-P

E10344

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E10341

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3900

0.1ml

Concentrate

M3902

0.5ml

M3904 M3901

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Mesothelioma

Control Tissue: Cerebellum, Kidney

Cellular Localization: Membrane

Cellular Localization: Cytoplasm Control Lysate: Human Kidney Molecular Weight: 28 kDa

Human Mesothelioma stained with anti-Cadherin-N/N-Cadherin antibody M390

Human Cerebellum stained with anti-Calbindin D28 antibody E1034

Product Description: Cadherin-N/N-cadherin is a 140kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion. The function of N-cadherin is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminus region of N-cadherin and the cytoplasmic catenin proteins.

Product Description: Calbindin-D-28K is a highly conserved calcium binding protein. It belongs to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.

Cadherin-N/N-Cadherin Rabbit Polyclonal

Calcitonin Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

M3170

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cadherin-N protein.

M3172

0.5ml

Concentrate

Immunogen: Synthetic human calcitonin 1-32 amino acid peptide.

1.0ml

Concentrate

Application: IHC-P

M3174

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3171

7.0ml

Ready-to-use

Catalog No.

Size

Format

E18260

0.1ml

Concentrate

E18262

0.5ml

E18264 E18261

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Adrenal Gland, Liver, Pancreas

Control Tissue: Thyroid, Thyroid Medullary Carcinoma

Cellular Localization: Membrane

Cellular Localization: Cytoplasm

Human Liver stained with anti-Cadherin-N/N-Cadherin antibody E1826

Human Thyroid Medullary Carcinoma stained with anti-Calcitonin antibody M317

Product Description: Cadherin-N/N-cadherin is a 140kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion. The function of N-cadherin is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminus region of N-cadherin and the cytoplasmic catenin proteins.

20

Clone: SP17

Product Description: Calcitonin is a 32 amino acid peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Calcitonin is expressed in a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Calcitonin Rabbit Polyclonal

Calpain Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18780

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic human calcitonin 1-32 amino acid peptide.

E18782

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human calpain protein.

1.0ml

Concentrate

Application: IHC-P, WB

E18784

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E18781

7.0ml

Ready-to-use

Catalog No.

Size

Format

E1150

0.1ml

Concentrate

E1152

0.5ml

E1154 E1151

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Thyroid, Thyroid Medullary Carcinoma

Control Tissue: Breast Carcinoma Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm Control Lysate: Human Thyroid Molecular Weight: 14 kDa

Human Breast Carcinoma stained with anti-Calpain antibody E1878

Human Thyroid stained with anti-Calcitonin antibody E115

Product Description: Calcitonin is a 32 amino acid peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Calcitonin is expressed in a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Product Description: Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes due to calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.

Calcium Sensing Receptor (CaSR) Rabbit Polyclonal

Calpastatin Rabbit Monoclonal Clone: SP82

Clone: N/A

Catalog No.

Size

Format

M3820

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from N-terminus of rat calcium sensing receptor.

M3822

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human calpastatin protein.

1.0ml

Concentrate

Application: IHC-P

M3824

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

M3821

7.0ml

Ready-to-use

Catalog No.

Size

Format

E10620

0.1ml

Concentrate

E10622

0.5ml

E10624 E10621

Species Reactivity: Human*

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Placenta Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Placenta stained with anti-Calcium Sensing Receptor (CaSR) antibody E1062

Human Breast Carcinoma stained with anti-Calpastatin antibody M382

Product Description: The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).

Product Description: Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. Calpastatin, a specific endogenous inhibitor of calpain, functions by binding to calpain. It contains mutliple inhibitory fragments for calpain activity.

Calpain Rabbit Monoclonal

Calpastatin Rabbit Polyclonal Clone: N/A

Clone: SP81

Catalog No.

Size

Format

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human calpain protein.

E18790 E18792

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human calpastatin protein.

1.0ml

Concentrate

Application: IHC-P, WB

E18794

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

E18791

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3810

0.1ml

Concentrate

M3812

0.5ml

M3814 M3811

Species Reactivity: Human*, Mouse*

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm Control Lysate: 293T Molecular Weight: 28 kDa

Human Breast Carcinoma stained with anti-Calpastatin antibody E1879

Human Breast Carcinoma stained with anti-Calpain antibody M381

Product Description: Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes due to calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.

Product Description: Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. Calpastatin, a specific endogenous inhibitor of calpain, functions by binding to calpain. It contains mutliple inhibitory fragments for calpain activity.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

21


Calretinin Rabbit Monoclonal

CaM Kinase Kinase alpha Rabbit Polyclonal Clone: SP13

Catalog No.

Size

Format

E13850

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant full length mouse calretinin protein.

E13852

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human CaM kinase kinase alpha.

1.0ml

Concentrate

Application: IHC-P

E13854

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E13851

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3130

0.1ml

Concentrate

M3132

0.5ml

M3134 M3131

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Mesothelioma

Control Tissue: Brain

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Nucleus

Human Mesothelioma stained with anti-Calretinin antibody M313

Human Brain stained with anti-CaM Kinase Kinase alpha antibody E1385

Product Description: Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation in Purkinje and basket cells and in neurons of the dentate nucleus. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.

Product Description: Ca2+/Calmodulin dependent protein kinases (CaM Kinase I, II and IV) are considered to play a central role in many cellular functions in response to Ca2+ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation (LTP), receptor function, modification of the cytoskeleton and regulation of gene expression.

Calretinin Rabbit Polyclonal

Carbonic Anhydrase 9 (CA-9) Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M4060

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant full length mouse calretinin protein.

M4062

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from human CA-9.

1.0ml

Concentrate

Application: IHC-P, WB

M4064

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M4061

7.0ml

Ready-to-use

Catalog No.

Size

Format

E1170

0.1ml

Concentrate

E1172

0.5ml

E1174 E1171

Clone: SP106

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Mesothelioma

Control Tissue: Stomach

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Membrane, Nucleus

Control Lysate: Human Brain Molecular Weight: 29 kDa Human Mesothelioma stained with anti-Calretinin antibody E117

Human Stomach stained with anti-Carbonic Anhydrase 9 (CA-9) antibody M406

Product Description: Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation in Purkinje and basket cells and in neurons of the dentate nucleus. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.

Product Description: Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. CA-9 is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. CA-9 is a reliable histochemical marker of hypoxia.

CaM Kinase IV Rabbit Polyclonal

Carbonic Anhydrase 9 (CA-9) Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E18420

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of rat CaM kinase IV.

E18422

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from human CA-9.

1.0ml

Concentrate

Application: IHC-P

E18424

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E18421

7.0ml

Ready-to-use

Catalog No.

Size

Format

E10600

0.1ml

Concentrate

E10602

0.5ml

E10604 E10601

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Stomach

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Membrane, Nucleus Control Lysate: Ramos Molecular Weight: 50 kDa

Human Brain stained with anti-CaM Kinase IV antibody E1060

Product Description: Calmodulin dependent protein kinases (CaM kinases) consist of a family of Ser/Thr protein kinases including CaMKI, CaMKII and CaMKIV. CaMKs play a central role in many cellular functions in response to Ca2+ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation etc. CaM kinase IV is abundantly expressed in the brain and in the thymus and is localized to the cytoplasm and nucleus.

22

Human Stomach stained with anti-Carbonic Anhydrase 9 (CA-9) antibody E1842

Product Description: Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux and it can be used as a histochemical marker of hypoxia.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies CAS Rabbit Polyclonal

Caspase-10 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E10850

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from N-terminus of human CAS.

E10852

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human caspase-10.

1.0ml

Concentrate

Application: IHC-P

E10854

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E10851

7.0ml

Ready-to-use

Catalog No.

Size

Format

E10810

0.1ml

Concentrate

E10812

0.5ml

E10814 E10811

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Liver Carcinoma

Control Tissue: Liver Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Liver Carcinoma stained with anti-CAS antibody E1081

Human Liver Carcinoma stained with anti-Caspase-10 antibody E1085

Product Description: CAS (Cellular Apoptosis Susceptibility) contains 971 amino acids with 59% overall sequence homology as compared to yeast chromosome segregation homolog, CSE1. CAS is highly expressed in testis and fetal liver. CAS expression in human fibroblasts increases as cells are induced to proliferate and decreases in growth arrested cells.

Product Description: Caspase-10 is a member of the Ced-3 cystein protease family and it has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli. Caspase-1 to-10 function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity.

Caspase-1 Rabbit Polyclonal

Catenin-beta Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2260

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human caspase-1 protein.

E2262

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of the catenin-beta protein.

1.0ml

Concentrate

Application: IHC-P

E2264

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E2261

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3910

0.1ml

Concentrate

E3912

0.5ml

E3914 E3911

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane Control Lysate: A431 Molecular Weight: 92 kDa

Human Placenta stained with anti-Caspase-1 antibody E391

Human Breast Carcinoma stained with anti-Catenin-beta antibody E226

Product Description: Caspase-1/ICE (Interleukin-1 Beta Converting Enzyme) is expressed as a proenzyme of 45kDa in many tissues and is activated by proteolytic cleavage. Active caspase-1 is a tetramer of two subunits p10 and p20. Caspase-1 (cystein aspartic-specific protease 1) cleaves interleukin beta into its active form which further exerts its proinflammatory effects. Overexpression of caspase-1 can induce apoptosis in fibroblasts.

Product Description: The catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. β-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between α-actin and α-catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.

Caspase-8 Rabbit Polyclonal

Catenin-gamma Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3680

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human caspase-8 protein.

E3682

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human catenin-gamma protein.

1.0ml

Concentrate

Application: IHC-P

E3684

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E3681

7.0ml

Ready-to-use

Catalog No.

Size

Format

E2280

0.1ml

Concentrate

E2282

0.5ml

E2284 E2281

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Stomach

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Stomach stained with anti-Caspase-8 antibody E228

Human Breast Carcinoma stained with anti-Catenin-gamma antibody E368

Product Description: Caspase-8 binds to the death effector domain (DED) through an analogous DED domain. Recruitment of caspase-8 to the Fas receptor results in oligomerization of the caspase-8 protein, which in turn drives its autoactivation through “self-cleavage.” Activated caspase-8 then activates other downstream caspases including caspase-9, thereby commiting the cell to undergo apoptosis. This antibody also detects the processed forms (42/44kDa, 25kDA, and 14kDa) of caspase-8.

Product Description: The catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin. α-catenin can also bind P-cadherin and N-cadherin. γ-catenin, also known as plakoglobin, is an 82kDa protein that binds with α-catenin and N-cadherin.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

23


Cathepsin D Rabbit Polyclonal

Caveolin-1 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Recombinant human cathepsin D protein.

E10780

0.1ml

Concentrate

Concentrate

Application: IHC-P

E10782

0.5ml

Concentrate

Immunogen: Synthetic peptide from N-terminus of human caveolin-1.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-use

Species Reactivity: Human*

E10784

7.0ml

E10781

7.0ml

Ready-to-use

Catalog No.

Size

Format

E2820

0.1ml

E2822

0.5ml

E2824 E2821

Isotype: Rabbit IgG

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma Cellular Localization: Cytoplasm

Control Tissue: Lung Carcinoma

Human Breast Carcinoma stained with anti-Cathepsin D antibody E282

Human Lung Carcinoma stained with anti-Caveolin-1 antibody E1078

Cellular Localization: Cytoplasm, Membrane

Product Description: Cathepsin D is synthesized as a 54kDa precursor and is proteolytically processed to an intermediate 48kDa single chain. The 48KDa form is further processed into the more stable 34kDa and 14kDa mature forms. Cathepsin D is an estrogen-regulated lysosomal protease that facilitates cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and connective tissue. Cathepsin D is expressed in epithelial cells as well as in macrophages.

Product Description: Caveolae are 50-100 nm flask-shaped subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules or receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae.

Caveolin-1 Rabbit Monoclonal

CBP/p300-Interacting Transactivator 1 (CITED-1) Rabbit Polyclonal

Clone: SP43

Catalog No.

Size

Format

E3510

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human caveolin-1 protein.

E3512

0.5ml

Concentrate

Immunogen: Recombinant full-length human CITED-1 protein.

1.0ml

Concentrate

Application: IHC-P

E3514

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*, Mouse*

E3511

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3430

0.1ml

Concentrate

M3432

0.5ml

M3434 M3431

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung, Lung Carcinoma Cellular Localization: Cytoplasm, Membrane

Control Tissue: Breast Carcinoma, Papillary Thyroid Carcinoma

Human Lung Carcinoma stained with anti-Caveolin-1 antibody M343

Human Breast Carcinoma stained with anti-CBP/p300-Interacting Transactivator 1 (CITED-1) antibody E351

Cellular Localization: Cytoplasm, Nucleus

Product Description: Caveolae are 50-100 nm flask-shaped subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules or receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae.

Product Description: CITED1 (CBP/p300-Interacting Transactivator 1)/Melanocytespecific protein 1 is highly expressed in most of the papillary thyroid carcinoma. It is not expressed in normal tissues except normal breast epithelium. Studies show that this protein functions as a selective coactivator for estrogen-dependent transcription.

Caveolin-1 Rabbit Polyclonal

CCR7 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18460

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human caveolin-1.

E18462

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from human CCR7.

1.0ml

Concentrate

Application: IHC-P

E18464

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E18461

7.0ml

Ready-to-use

Catalog No.

Size

Format

E17640

0.1ml

Concentrate

E17642

0.5ml

E17644 E17641

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung, Lung Carcinoma

Control Tissue: Small Intestine

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Membrane

Human Lung stained with anti-Caveolin-1 antibody E1764

Human Small Intestine stained with anti-CCR7 antibody E1846

Product Description: Caveolae are 50-100 nm flask-shaped subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules or receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae.

24

Clone: N/A

Product Description: C-C chemokine receptor type 7 (CCR7) is a member of the G protein coupled receptor family (subfamily: chemokine). This receptor was identified as a gene induced by the Epstein Barr virus (EBV). It is expressed particularly in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies CCR8 Rabbit Polyclonal

CD3 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3070

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from aa 183-193 of human CCR8.

M3072

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

M3074

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

M3071

7.0ml

Ready-to-use

Catalog No.

Size

Format

E10790

0.1ml

Concentrate

E10792

0.5ml

E10794 E10791

Clone: SP7 Immunogen: Synthetic peptide corresponding to internal region of the epsilon chain of human CD3 protein. Application: IHC-P, WB Species Reactivity: Human*, Mouse*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Thymus

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane Control Lysate: Jurkat Molecular Weight: 20 kDa

Human Thymus stained with anti-CCR8 antibody E1079

Human Tonsil stained with anti-CD3 antibody M307

Product Description: C-C chemokine receptor type 8 (CCR8) encodes a member of the beta chemokine receptor family. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP1 beta) have been identified as ligands of this receptor. CCR8 expression has been reported in spleen and thymus, natural-killer cells, monocytes, and T cells, and at lower levels in peripheral blood leukocytes.

Product Description: This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

CD1 alpha Rabbit Polyclonal

CD3 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E1250

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 19-327 of human CD1 alpha.

E1252

0.5ml

Concentrate

1.0ml

Concentrate

Application: WB

E1254

1.0ml

Concentrate

N/A

N/A

Species Reactivity: Human*

E1251

7.0ml

Ready-to-use

Catalog No.

Size

Format

E4710

0.1ml

Concentrate

E4712

0.5ml

E4714 N/A

Clone: N/A Immunogen: Synthetic 13-mer peptide corresponding to internal region of the epsilon chain of human CD3 protein. Application: IHC-P, WB Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: N/A

Control Tissue: Tonsil

Cellular Localization: N/A

Cellular Localization: Membrane

Control Lysate: K562 Molecular Weight: 49 kDa

Control Lysate: Jurkat

N/A E471

Human Tonsil stained with anti-CD3 antibody E125

Molecular Weight: 19 kDa

Product Description: At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 is expressed on cortical thymocytes, Langerhans cells, and dendritic cells. It is absent on mature peripheral blood T cells but intracytoplasmic expression is detected on activated T lymphocytes. CD1 proteins have been demonstrated to restrict T-cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation.

Product Description: This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

CD2 Rabbit Polyclonal

CD3 zeta Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3050

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 25-209 of human CD2 .

E3052

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of the zeta chain of human CD3 protein.

1.0ml

Concentrate

Application: IHC-P, WB

E3054

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E3051

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3040

0.1ml

Concentrate

E3042

0.5ml

E3044 E3041

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Control Lysate: Jurkat Molecular Weight: 45-58 kDa Human Tonsil stained with anti-CD2 antibody E304

Product Description: The CD2 antigen (LFA-2) is a monomeric 45 to 58kDa glycoprotein. It is an accessory molecule important in mediating the adhesion of activated T cells and thymocytes with antigen-presenting cells and target cells. This antibody reacts with the majority of T cells in peripheral lymphoid tissue, NK cells, cortical thymocytes and most malignant cells of T cell origin.

Human Tonsil stained with anti-CD3 zeta antibody E305

Product Description: CD3 consists of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. The zeta chain contains three consecutive copies of the same motif.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

25


CD4 Rabbit Monoclonal

CD5 Rabbit Polyclonal Clone: SP35

Catalog No.

Size

Format

E2470

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human CD4.

E2472

0.5ml

Concentrate

Immunogen: Synthetic peptide from the intracellular region of CD5.

1.0ml

Concentrate

Application: IHC-P

E2474

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E2471

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3350

0.1ml

Concentrate

M3352

0.5ml

M3354 M3351

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: T Cell Lymphoma, Tonsil

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Tonsil stained with anti-CD4 antibody M335

Human Tonsil stained with anti-CD5 antibody E247

Product Description: CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV and is expressed in T-cell lymphomas.

Product Description: CD5, a transmembrane protein, is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphoma are CD5-.

CD4 Rabbit Polyclonal

CD7** Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3940

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human CD4.

M3942

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human CD7 protein.

1.0ml

Concentrate

Application: IHC-P

M3944

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3941

7.0ml

Ready-to-use

Catalog No.

Size

Format

E17680

0.1ml

Concentrate

E17682

0.5ml

E17684 E17681

Clone: SP94

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Tonsil stained with anti-CD4 antibody E1768

Human Tonsil stained with anti-CD7 antibody M394

Product Description: CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV and is expressed in T-cell lymphomas.

Product Description: CD7 (gp40, Leu9) is a member of the immunoglobulin gene superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T cell interactions and also in T cell/B cell interactions during early lymphoid development. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on maligant B cells.

CD5 Rabbit Monoclonal

CD7 Rabbit Polyclonal Clone: SP19

Catalog No.

Size

Format

E18930

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from the intracellular region of human CD5.

E18932

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human CD7 protein.

1.0ml

Concentrate

Application: IHC-P

E18934

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E18931

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3190

0.1ml

Concentrate

M3192

0.5ml

M3194 M3191

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Tonsil stained with anti-CD5 antibody M319

Human Tonsil stained with anti-CD7 antibody E1893

Product Description: CD5, a transmembrane protein, is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphoma are CD5-.

26

Clone: N/A

Product Description: CD7 (gp40, Leu9) is a member of the immunoglobulin gene superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T cell interactions and also in T cell/B cell interactions during early lymphoid development. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on maligant B cells.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies CD8 Rabbit Monoclonal

CD10** Rabbit Monoclonal Clone: SP16

Catalog No.

Size

Format

M3670

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of alpha chain of the human CD8 molecule.

M3672

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to human CD10.

1.0ml

Concentrate

Application: IHC-P

M3674

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

M3671

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3160

0.1ml

Concentrate

M3162

0.5ml

M3164 M3161

Clone: SP67

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Renal Cell Carcinoma, Tonsil

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane Control Lysate: Ramos Molecular Weight: 100 kDa

Human Tonsil stained with anti-CD8 antibody M316

Human Renal Cell Carcinoma stained with anti-CD10 antibody M367

Product Description: The CD8 molecule consists of two chains, termed α and β chain, which are expressed as a disulfide-linked α/β heterdimer or as an α/α homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as α/β heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 α chain (but not β chain).

Product Description: CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular lymphomas, immature B cells, chronic myelocytic leukemia, and renal clear cell carcinoma. It is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border kidney, and gut epithelial cells.

CD8 Rabbit Polyclonal

CD10 Rabbit Polyclonal

Catalog No.

Size

Format

E2480

0.1ml

Concentrate

E2482

0.5ml

Concentrate

E2484

1.0ml

Concentrate

E2481

7.0ml

Ready-to-use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to C-terminus of the cytoplasmic domain of alpha chain of the CD8 molecule.

E18270

0.1ml

Concentrate

E18272

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human CD10.

E18274

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P

E18271

7.0ml

Ready-to-use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Kidney, Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Membrane

Human Tonsil stained with anti-CD8 antibody E248

Human Kidney stained with anti-CD10 antibody E1827

Product Description: The CD8 molecule consists of two chains, termed α and β chain, which are expressed as a disulfide-linked α/β heterdimer or as an α/α homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as α/β heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 α chain (but not β chain).

Product Description: CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular lymphomas, immature B cells, chronic myelocytic leukemia, and renal clear cell carcinoma. It is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border kidney, and gut epithelial cells.

CD9 Rabbit Polyclonal

CD19 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M4100

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant human full-length CD9 protein.

M4102

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human CD19.

1.0ml

Concentrate

Application: IHC-P

M4104

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M4101

7.0ml

Ready-to-use

Catalog No.

Size

Format

E11010

0.1ml

Concentrate

E11012

0.5ml

E11014 E11011

Clone: SP110

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Tonsil stained with anti-CD9 antibody E1101

Human Tonsil stained with anti-CD19 antibody M410

Product Description: The CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. In pre-B cells and platelets, CD9 antigen regulates cell activation aggregation possibly through an association with the integrin CD41/CD61. It also regulates cell motility in a variety of cell lines.

Product Description: CD19 is expressed in B lymphocytes and B cell lymphoma. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. CD19 is negative in T lymphocytes, plasma cells, and their tumors.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

27


CD19 Rabbit Polyclonal

CD22** Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M4040

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human CD19 protein.

M4042

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human CD22.

1.0ml

Concentrate

Application: IHC-P

M4044

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M4041

7.0ml

Ready-to-use

Catalog No.

Size

Format

E18960

0.1ml

Concentrate

E18962

0.5ml

E18964 E18961

Clone: SP104

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane

Human Tonsil stained with anti-CD19 antibody E1896

Human Tonsil stained with anti-CD22 antibody M404

Product Description: CD19 is expressed in B lymphocytes and B-cell lymphoma. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. CD19 is negative in T lymphocytes, plasma cells, and their tumors.

Product Description: CD22 is a type 1 integral membrane glycoprotein expressed in both the cytoplasm and cell membrane of B lymphocytes. CD22 antigen appears early in B cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. CD22 membrane expression is limited to the late differentiation stages. There are two different isoforms of CD22. It exists as a monomer and heterodimer. CD22 is strongly expressed in hairy cell leukemia.

CD20 Rabbit Monoclonal

CD22 Rabbit Polyclonal Clone: SP32

Catalog No.

Size

Format

E19020

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human CD20 protein.

E19022

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human CD22.

1.0ml

Concentrate

Application: IHC-P, WB

E19024

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E19021

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3320

0.1ml

Concentrate

M3322

0.5ml

M3324 M3321

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: B Cell Lymphoma, Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Membrane

Control Lysate: Ramos Molecular Weight: 35 kDa Human B Cell Lymphoma stained with anti-CD20 antibody M332

Human Tonsil stained with anti-CD22 antibody E1902

Product Description: CD20 is a leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.

Product Description: CD22 is a type 1 integral membrane glycoprotein expressed in both the cytoplasm and cell membrane of B lymphocytes. CD22 antigen appears early in B cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. CD22 membrane expression is limited to the late differentiation stages. There are two different isoforms of CD22. It exists as a monomer and heterodimer. CD22 is strongly expressed in hairy cell leukemia.

CD20 Rabbit Polyclonal

CD23 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3230

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human CD20 protein.

M3232

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 48-248 of human CD23.

1.0ml

Concentrate

Application: IHC-P, WB

M3234

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

M3231

7.0ml

Ready-to-use

Catalog No.

Size

Format

E2560

0.1ml

Concentrate

E2562

0.5ml

E2564 E2561

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: B Cell Lymphoma, Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Membrane

Control Lysate: Raji

Molecular Weight: 45 kDa

Molecular Weight: 35 kDa Human Tonsil stained with anti-CD20 antibody E256

Product Description: CD20 is a leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loophelix leucine zipper transcription factors.

28

Clone: SP23

Control Lysate: Raji

Human Tonsil stained with anti-CD23 antibody M323

Product Description: CD23 is a 45kDa glycoprotein, which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukemia and some cases of centroblastic/ centrocytic lymphoma.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies CD23 Rabbit Polyclonal

CD31 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E11110

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 48-248 of human CD23.

E11112

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of mouse CD31 protein.

1.0ml

Concentrate

Application: IHC-P

E11114

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E11111

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3020

0.1ml

Concentrate

E3022

0.5ml

E3024 E3021

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Tonsil stained with anti-CD23 antibody E302

Human Tonsil stained with anti-CD31 antibody E1111

Product Description: CD23 is a 45kDa glycoprotein, which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukemia and some cases of centroblastic/ centrocytic lymphoma.

Product Description: CD31 is a glycoprotein expressed on endothelial cells and in platelets. It is known to be involved in cell signaling and cell adhesion.

CD30 Rabbit Polyclonal

CD34 (Endothelial Cell Marker) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E17540

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human CD30.

E17542

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to human CD34 protein.

1.0ml

Concentrate

Application: IHC-P

E17544

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E17541

7.0ml

Ready-to-use

Catalog No.

Size

Format

E17410

0.1ml

Concentrate

E17412

0.5ml

E17414 E17411

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Hodgkin’s Lymphoma, Tonsil

Control Tissue: Angiosarcoma, Lung, Tonsil

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane

Human Hodgkin’s Lymphoma stained with anti-CD30 antibody E1741

Human Angiosarcoma stained with anti-CD34 (Endothelial Cell Marker) antibody E1754

Product Description: CD30, a single chain glycoprotein, is synthesized as a 90kDa precursor which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. The CD30/Ki-1 antigen is expressed by mononuclear Hodgkin and multinucleated Reed-Sternberg cells in Hodgkin’s disease, by the tumor cells of a majority of anaplastic large cell lymphomas, and by a varying proportion of activated T and B cells.

Product Description: CD34, a single chain transmembrane glycoprotein, is selectively expressed on human lymphoid and myeloid hematopoietic progenitor cells.

CD31 Rabbit Monoclonal

CD34 (Endothelial Cell Marker) Mouse Monoclonal Clone: SP38

Catalog No.

Size

Format

E1280

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of mouse CD31 protein.

E1282

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E1284

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*, Mouse*

E1281

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3380

0.1ml

Concentrate

M3382

0.5ml

M3384 M3381

Clone: QBEnd/10 Immunogen: Detergent solubilized vesicular suspension prepared from a perfusate of human term placenta. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgG1/k

Control Tissue: Tonsil Cellular Localization: Membrane

Control Tissue: Lung, Tonsil

Human Tonsil stained with anti-CD31 antibody M338

Human Tonsil stained with anti-CD34 (Endothelial Cell Marker) antibody E128

Product Description: CD31 is a glycoprotein expressed on endothelial cells and in platelets. It is known to be involved in cell signaling and cell adhesion.

Cellular Localization: Cytoplasm, Membrane

Product Description: CD34, a single chain transmembrane glycoprotein, is selectively expressed on human lymphoid and myeloid hematopoietic progenitor cells.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

29


CD38 Rabbit Polyclonal

CD43 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E17950

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide near the C-terminus of human CD38.

E17952

0.5ml

Concentrate

Immunogen: Synthetic peptide from internal region of human CD43 protein.

1.0ml

Concentrate

Application: IHC-P, WB

E17954

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E17951

7.0ml

Ready-to-use

Catalog No.

Size

Format

E19170

0.1ml

Concentrate

E19172

0.5ml

E19174 E19171

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Membrane

Control Lysate: HeLa Molecular Weight: 46 kDa (predicted) Human Tonsil stained with anti-CD38 antibody E1917

Human Tonsil stained with anti-CD43 antibody E1795

Product Description: CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a transmembrane glycoprotein found on the surface of many immune cells including plasma cells, activated or immature T and B cells, monocytes, and natural killer cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. CD38 is used as one of the plasma cell markers and its ligand is CD31 molecules.

Product Description: CD43 is one of the major glycoproteins of thymocytes and T lymphocytes. It plays a role in the physiochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rod-like structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site.

CD40 Rabbit Polyclonal

CD44 Std** Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3370

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human CD40.

M3372

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to human CD44.

1.0ml

Concentrate

Application: IHC-P

M3374

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

M3371

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3700

0.1ml

Concentrate

E3702

0.5ml

E3704 E3701

Clone: SP37

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Hodgkin’s Lymphoma, Tonsil

Control Tissue: Esophageal Carcinoma

Cellular Localization: Membrane

Cellular Localization: Membrane Control Lysate: HeLa Molecular Weight: 80 kDa

Human Hodgkin’s Lymphoma stained with anti-CD40 antibody E370

Human Esophageal Carcinoma stained with anti-CD44 Std antibody M337

Product Description: The CD40 antigen is a single chain glycoprotein. It is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin’s disease associated antigen, CD30. CD40 is present on all B cells except plasma cells. It is also found on some epithelial cells, carcinomas and lymphoid dentritic cells.

Product Description: CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis.

CD43 Rabbit Monoclonal

CD44 Std Rabbit Polyclonal Clone: SP55

Catalog No.

Size

Format

E17370

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from the human CD43.

E17372

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human CD44.

1.0ml

Concentrate

Application: IHC-P, WB

E17374

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E17371

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3550

0.1ml

Concentrate

M3552

0.5ml

M3554 M3551

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Esophageal Carcinoma

Cellular Localization: Membrane

Cellular Localization: Membrane

Control Lysate: Jurkat Molecular Weight: 95/115/135 kDa Human Tonsil stained with anti-CD43 antibody M355

Product Description: CD43 is one of the major glycoproteins of thymocytes and T lymphocytes. It plays a role in the physiochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site.

30

Human Esophageal Carcinoma stained with anti-CD44 Std antibody E1737

Product Description: CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies CD44 Std Mouse Monoclonal

CD56 Mouse Monoclonal Clone: SPM521

Catalog No.

Size

Format

E1370

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to human CD44.

E1372

0.5ml

Concentrate

Immunogen: Membrane preparation of a small cell lung carcinoma.

1.0ml

Concentrate

Application: IHC-P, WB

E1374

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E1371

7.0ml

Ready-to-use

Catalog No.

Size

Format

E17360

0.1ml

Concentrate

E17362

0.5ml

E17364 E17361

Clone: SPM128

Species Reactivity: Human*

Isotype: Mouse IgG2a

Isotype: Mouse IgG1/k

Control Tissue: Esophageal Carcinoma

Control Tissue: Brain, Neuroblastoma

Cellular Localization: Membrane

Cellular Localization: Membrane

Control Lysate: HeLa Molecular Weight: 80 kDa Human Esophageal Carcinoma stained with anti-CD44 Std antibody E1736

Human Neuroblastoma stained with anti-CD56 antibody E137

Product Description: CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis.

Product Description: Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. NCAM (CD56) is reported to express on most neuroectodermal derived cell lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.

CD45/T200/LCA Mouse Monoclonal

CD56 Mouse Monoclonal

Clone: SPM496

Catalog No.

Size

Format

Concentrate

Immunogen: Stimulated human leucocytes.

E11100

0.1ml

Concentrate

Concentrate

Application: IHC-P

E11102

0.5ml

Concentrate

Immunogen: Membrane preparation of a small cell lung carcinoma.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-use

Species Reactivity: Human*

E11104

7.0ml

E11101

7.0ml

Ready-to-use

Catalog No.

Size

Format

E12430

0.1ml

E12432

0.5ml

E12434 E12431

Isotype: Mouse IgG1/k

Clone: 123C3

Species Reactivity: Human* Isotype: Mouse IgG1/k

Control Tissue: Tonsil Cellular Localization: Membrane

Control Tissue: Brain, Neuroblastoma

Human Tonsil stained with anti-CD45/T200/LCA antibody E1243

Human Neuroblastoma stained with anti-CD56 antibody E1110

Cellular Localization: Membrane

Product Description: CD45 leucocyte common antigen (LCA) belongs to a family of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines and normal and malignant non-hematopoietic tissues. The intracellular portion of these molecules has protein phosphatase activity and is involved in regulation of transmembrane signals.

Product Description: Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. NCAM (CD56) is reported to express on most neuroectodermal derived cell lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.

CD45RA Mouse Monoclonal

CD63 Mouse Monoclonal Clone: SPM504

Catalog No.

Size

Format

Concentrate

Immunogen: Stimulated human leucocytes.

E17510

0.1ml

Concentrate

Immunogen: Recombinant human CD63 protein.

0.5ml

Concentrate

Application: IHC-P

E17512

0.5ml

Concentrate

Application: IHC-P

E16484

1.0ml

Concentrate

E17514

1.0ml

Concentrate

E16481

7.0ml

Ready-to-use

Species Reactivity: Human*

E17511

7.0ml

Ready-to-use

Species Reactivity: Human*

Catalog No.

Size

Format

E16480

0.1ml

E16482

Isotype: Mouse IgG2a

Clone: SPM524

Isotype: Mouse IgG1/k

Control Tissue: Tonsil

Control Tissue: Melanoma

Cellular Localization: Membrane

Cellular Localization: Cytoplasm

Human Tonsil stained with anti-CD45RA antibody E1648

Human Melanoma stained with anti-CD63 antibody E1751

Product Description: CD45 or Leukocyte Common Antigen (LCA/T200) is a membrane sialoglycoprotein. The differential splicing of the exons generates various isoforms. CD45RA is a single chain glycoprotein. It is expressed on appoximately 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive T-cells while CD45RO+ cells are memory T-cells.

Product Description: CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

31


CD68 Rabbit Polyclonal

CD74 Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E17420

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide in the internal region of human CD68 protein.

E17422

0.5ml

Concentrate

Immunogen: Recombinant human CD74 protein.

1.0ml

Concentrate

Application: IHC-P

E17424

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E17421

7.0ml

Ready-to-use

Catalog No.

Size

Format

E19190

0.1ml

Concentrate

E19192

0.5ml

E19194 E19191

Clone: SPM523

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgG1/k

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Membrane Control Lysate: Ramos Molecular Weight: 35 kDa

Human Tonsil stained with anti-CD68 antibody E1919

Human Tonsil stained with anti-CD74 antibody E1742

Product Description: CD68 is an intracellular glycoprotein mainly expressed on macrophages and monocytes. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is also expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma.

Product Description: Expression of the invariant chain of the HLA-DR antigen (CD74) by B-lymphocytes begins in the pre-B stage but lost before the final differentiation to plasma cells. This antibody is expressed in B-lymphocytes and many B cell lymphomas.

CD68 Rabbit Polyclonal

CD79a Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3180

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide near the C-terminus of human CD68 protein.

M3182

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus region of human CD79a protein.

1.0ml

Concentrate

Application: IHC-P, WB

M3184

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3181

7.0ml

Ready-to-use

Catalog No.

Size

Format

E19160

0.1ml

Concentrate

E19162

0.5ml

E19164 E19161

Clone: SP18

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: B Cell Lymphoma, Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Control Lysate: Raji, SH-SY5Y Molecular Weight: 37 kDa Protein (calculated); 110 kDa (apparent) Human Tonsil stained with anti-CD68 antibody E1916

Human B Cell Lymphoma stained with anti-CD79a antibody M318

Product Description: CD68 is an intracellular glycoprotein mainly expressed on macrophages and monocytes. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is also expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma.

Product Description: A disulphide-linked heterodimer, consisting of CD79a/mb-1 and CD79b/B29 polypeptides, CD79a is non-covalently associated with membrane-bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas.

CD68 Rabbit Polyclonal

CD79a Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2490

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 155-355 of human CD68.

E2492

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human CD79a protein.

1.0ml

Concentrate

Application: WB

E2494

1.0ml

Concentrate

Application: IHC-P, WB

N/A

N/A

Species Reactivity: Human*

E2491

7.0ml

Ready-to-use

Catalog No.

Size

Format

E13920

0.1ml

Concentrate

E13922

0.5ml

E13924 N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: N/A

Control Tissue: B Cell Lymphoma, Tonsil

Cellular Localization: N/A

Cellular Localization: Membrane

Control Lysate: Human Brain

Control Lysate: Raji

Molecular Weight: 38 kDa

Molecular Weight: 44-48 kDa

N/A E1392

Human Tonsil stained with anti-CD79a antibody E249

Product Description: CD68 is an intracellular glycoprotein expressed on macrophages and monocytes. CD68 expression has been demonstrated in T cells, NK cells, liver and renal tubules.

32

Clone: N/A

Product Description: A disulphide-linked heterodimer, consisting of CD79a/mb-1 and CD79b/B29 polypeptides, CD79a is non-covalently associated with membrane-bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies CD95/FAS Rabbit Polyclonal

CD105/Endoglin/TGF 1/3 Receptor Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E4230

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human FAS protein.

E4232

0.5ml

Concentrate

Immunogen: Recombinant protein encoding 26-176 aa of human CD105.

1.0ml

Concentrate

Application: IHC-P

E4234

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E4231

7.0ml

Ready-to-use

Catalog No.

Size

Format

E2500

0.1ml

Concentrate

E2502

0.5ml

E2504 E2501

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Small Intestine

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Colon stained with anti-CD95/FAS antibody E250

Human Tonsil stained with anti-CD105/Endoglin/TGF 1/3 Receptor antibody E423

Product Description: CD95, also known as FAS, is a transmembrane glycoprotein. It is a member of the nerve growth factor receptor/tumor necrosis factor superfamily. This cell surface molecule mediates receptor-triggered apoptosis. The FAS antigen is expressed on the surface of various cell types, including activated T and B lymphocytes and T lymphoblastoid cell lines.

Product Description: CD105 or endoglin or GP160 is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists as an O- and N-glycosylated homodimer. Up-regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation-associated endothelial cell marker. CD105 binds to TGF-Beta1 and Beta3 with high affinity but not TGF-Beta2.

CD99 Rabbit Monoclonal

CD117/c-kit Rabbit Monoclonal Clone: SP119

Catalog No.

Size

Format

M3260

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant full length human CD99 protein.

M3262

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to cytoplasmic domain of human CD117 protein.

1.0ml

Concentrate

Application: IHC-P

M3264

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3261

7.0ml

Ready-to-use

Catalog No.

Size

Format

M4190

0.1ml

Concentrate

M4192

0.5ml

M4194 M4191

Clone: SP26

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas

Control Tissue: Gastrointestinal Stromal Tumor

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Human Pancreas stained with anti-CD99 antibody M419

Human Gastrointestinal Stromal Tumor stained with anti-CD117/c-kit antibody M326

Product Description: CD99, or MIC2 gene product, or E2 antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. The antigen is also expressed by most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors.

Product Description: CD117, also called KIT or c-kit receptor, is a cytokine receptor on the surface of hematopoietic stem cells and other cells. CD117 gene encodes the human homolog of the proto-oncogene c-kit. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, and chronic myelogenous leukemia.

CD99/MIC2 Rabbit Polyclonal

CD117/c-kit Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E1440

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant full length human CD99 protein.

E1442

0.5ml

Concentrate

Immunogen: Synthetic peptide from the cytoplasmic domain of human CD117/c-kit protein.

1.0ml

Concentrate

Application: IHC-P, WB

E1444

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E1441

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3390

0.1ml

Concentrate

E3392

0.5ml

E3394 E3391

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas

Control Tissue: Gastrointestinal Stromal Tumor

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Control Lysate: Jurkat Molecular Weight: 32/28 kDa Human Pancreas stained with anti-CD99/MIC2 antibody E339

Product Description: CD99, or MIC2 gene product, or E2 antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. The antigen is also expressed by most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors.

Human Gastrointestinal Stromal Tumor stained with anti-CD117/c-kit antibody E144

Product Description: CD117, also called KIT or c-kit receptor, is a cytokine receptor on the surface of hematopoietic stem cells and other cells. CD117 gene encodes the human homolog of the proto-oncogene c-kit. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, and chronic myelogenous leukemia.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

33


CD138 Rabbit Polyclonal

CD163 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3960

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 18-218 human CD138.

M3962

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to near N-terminus of human CD163 protein.

1.0ml

Concentrate

Application: IHC-P

M3964

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3961

7.0ml

Ready-to-use

Catalog No.

Size

Format

E4560

0.1ml

Concentrate

E4562

0.5ml

E4564 E4561

Clone: SP96

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Lung

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Tonsil stained with anti-CD138 antibody E456

Human Lung stained with anti-CD163 antibody M396

Product Description: CD138/Syndecan-1 is a transmembrane heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycan. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre-B cells and plasma cells but is absent from mature B cells. It is lost from the apoptotic myeloma cells.

Product Description: CD163, a 130kDa membrane glycoprotein, is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone.

CD146 Rabbit Polyclonal

CD163 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18680

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant human CD146 protein encoding aa 433-647 expressed in E. coli.

E18682

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human CD163 protein.

1.0ml

Concentrate

Application: IHC-P

E18684

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E18681

7.0ml

Ready-to-use

Catalog No.

Size

Format

E11130

0.1ml

Concentrate

E11132

0.5ml

E11134 E11131

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Angiosarcoma

Control Tissue: Skin, Thymus

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Angiosarcoma stained with anti-CD146 antibody E1113

Human Skin stained with anti-CD163 antibody E1868

Product Description: CD146, also known as the melanoma cell adhesion molecule (MCAM), is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. CD146 belongs to the immunoglobulin super-gene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions.

Product Description: CD163, a 130kDa membrane glycoprotein, is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone.

CD150 Rabbit Polyclonal

CD168 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18740

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 1-136 of human CD150.

E18742

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human CD168 protein.

1.0ml

Concentrate

Application: WB

E18744

1.0ml

Concentrate

Application: IHC-P

N/A

N/A

Species Reactivity: Human*

E18741

7.0ml

Ready-to-use

Catalog No.

Size

Format

E13940

0.1ml

Concentrate

E13942

0.5ml

E13944 N/A

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: N/A

Control Tissue: Prostate Carcinoma

Cellular Localization: N/A

Cellular Localization: Membrane

Control Lysate: Human Heart Molecular Weight: 37 kDa N/A E1394

Product Description: CD150, designated SLAM (Signaling Lymphocyte Activation Molecule) or CDw150, belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, and the cytoplasmic isoforms of SLAM.

34

Human Prostate Carcinoma stained with anti-CD168 antibody E1874

Product Description: The CD168 antigen is a non-integral cell surface hyaluronan receptor and intracellular protein. When hyaluronan binds to CD168, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. CD168 activation regulates various cellular processes including cell migration, proliferation, signaling, regulation of gene expression, cell differentiation, morphogenesis, and metastasis.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies CD274/B7-H1 Rabbit Polyclonal

CDC2 p34 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E4150

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to near N-terminus of human CD274.

E4152

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E4154

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E4151

7.0ml

Ready-to-use

Catalog No.

Size

Format

E18360

0.1ml

Concentrate

E18362

0.5ml

E18364 E18361

Clone: N/A Immunogen: Synthetic peptide derived from C-terminus of human CDC2 p34 protein. Application: IHC-P, WB Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung, Skeletal Muscle, Tonsil

Control Tissue: Breast Carcinoma, Tonsil

Cellular Localization: Membrane

Cellular Localization: Nucleus Control Lysate: HeLa Molecular Weight: 34 kDa

Human Tonsil stained with anti-CD274/B7-H1 antibody E1836

Human Tonsil stained with anti-CDC2 p34 antibody E415

Product Description: B7-H1 (B7 homologue 1, PD-L1), expressed on the surface of tumor cells or antigen-presenting cells in the tumor microenvironment, can suppress antitumor activity in immune reaction, promote tumor growth and help tumor cells to escape the immune response. B7-H1 is highly expressed on leukemia cells of acute monocyte leukemia (M5). B7-H1 expression also plays a critical role in the pathogenesis of human gastric carcinoma.

Product Description: In vertebrates, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the CDC2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. CDC2 p34 is essential for the G2 to M transition in vertebrate cells. Several additional CDC2 p34-related cyclin dependent kinases have been identified.

CD278/ICOS Rabbit Monoclonal

CDH1 Rabbit Polyclonal

Clone: SP98

Catalog No.

Size

Format

E18830

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human CD278.

E18832

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human CDH1 protein.

1.0ml

Concentrate

Application: IHC-P

E18834

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E18831

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3980

0.1ml

Concentrate

M3982

0.5ml

M3984 M3981

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Reactive Lymph Node, Tonsil

Control Tissue: Rectal Carcinoma

Cellular Localization: Membrane

Cellular Localization: Nucleus

Human Reactive Lymph Node stained with anti-CD278/ICOS antibody M398

Human Rectal Carcinoma stained with anti-CDH1 antibody E1883

Product Description: CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T cells. It is essential both for efficient interaction between T and B cells and for normal antibody responses to T cell dependent antigens. It enhances all basic T cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells.

Product Description: CDH1 protein is a limiting, substrate-specific activator of anaphase-promoting complex (APC)-dependent proteolysis and is required for the degradation of the APC substrates, such as CIB2 and ASE1. CDH1 plays an important role in mitotic regulation.

CD278/ICOS Rabbit Polyclonal

CDK2 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3800

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human CD278 protein.

M3802

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

M3804

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

M3801

7.0ml

Ready-to-use

Catalog No.

Size

Format

E18650

0.1ml

Concentrate

E18652

0.5ml

E18654 E18651

Clone: SP80 Immunogen: Synthetic peptide corresponding to C-terminus of human CDK2 protein. Application: IHC-P, WB Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Nucleus Control Lysate: HeLa Molecular Weight: 33 kDa

Human Tonsil stained with anti-CD278/ICOS antibody E1865

Product Description: CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T cells. It is essential both for efficient interaction between T and B cells and for normal antibody responses to T cell dependent antigens. It enhances all basic T cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells.

Human Tonsil stained with anti-CDK2 antibody M380

Product Description: CDK2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae CDC28, and S. pombe CDC2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B).

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

35


CDK2 Rabbit Polyclonal

CEA/CD66e Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2970

0.1ml

Concentrate

Immunogen: Purified human CEA.

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human CDK2 protein.

E2972

0.5ml

Concentrate

Concentrate

Application: IHC-P, WB

Application: IHC-P

1.0ml

E2974

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E2971

7.0ml

Ready-to-use

Species Reactivity: Human*

Catalog No.

Size

Format

E18540

0.1ml

Concentrate

E18542

0.5ml

E18544 E18541

Isotype: Rabbit IgG

Clone: N/A

Isotype: Rabbit IgG Control Tissue: Colon Carcinoma

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Luminal Membrane

Cellular Localization: Cytoplasm, Nucleus Control Lysate: HeLa Molecular Weight: 33 kDa Human Tonsil stained with anti-CDK2 antibody E1854

Human Colon Carcinoma stained with anti-CEA/CD66e antibody E297

Product Description: CDK2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae CDC28, and S. pombe CDC2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B).

Product Description: Carcinoembryonic antigen (CEA), is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. CEA is expressed in various metastatic adenocarcinomas and in pulmonary adenocarcinomas.

CDX2 Rabbit Monoclonal

CEA/CD66e Mouse Monoclonal Clone: SP54

Catalog No.

Size

Format

E16500

0.1ml

Concentrate

Immunogen: Purified human CEA.

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human CDX2.

E16502

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P

1.0ml

E16504

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E16501

7.0ml

Ready-to-use

Species Reactivity: Human*

Catalog No.

Size

Format

M3540

0.1ml

Concentrate

M3542

0.5ml

M3544 M3541

Isotype: Rabbit IgG

Clone: SPM506

Isotype: Mouse IgG1 Control Tissue: Colon Carcinoma

Control Tissue: Colon Carcinoma

Cellular Localization: Cytoplasm, Luminal Membrane

Cellular Localization: Nucleus

Human Colon Carcinoma stained with anti-CDX2 antibody M354

Human Colon Carcinoma stained with anti-CEA/CD66e antibody E1650

Product Description: CDX2 encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, colorectal carcinomas, intestinal metaplasia of the stomach, and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa.

Product Description: Carcinoembryonic antigen (CEA), is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. CEA is expressed in various metastatic adenocarcinomas and in pulmonary adenocarcinomas.

CDX2 Rabbit Polyclonal

c-erbB-2 (Paired 1248) Rabbit Polyclonal

Catalog No.

Size

Format

E14180

0.1ml

Concentrate

E14182

0.5ml

Concentrate

E14184

1.0ml

Concentrate

N/A

N/A

N/A

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to N-terminus of human CDX2.

E18480

0.1ml

Concentrate

E18482

0.5ml

Concentrate

E18484

1.0ml

Concentrate

Application: WB

E18481

7.0ml

Ready-to-use

Clone: N/A Immunogen: Synthetic peptide derived from the region of human c-erbB-2 that contains non-phosphorylated tyrosine 1248. Application: IHC-P

Species Reactivity: Human*

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: N/A

Control Tissue: Breast Carcinoma

Cellular Localization: N/A

Cellular Localization: Membrane

Control Lysate: Human Placenta Molecular Weight: 37 kDa N/A E1418

Product Description: CDX2 encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, colorectal carcinomas, intestinal metaplasia of the stomach, and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa.

36

Human Breast Carcinoma stained with anti-c-erbB-2 (Paired 1248) antibody E1848

Product Description: c-erbB-2 is a 185kDa transmembrane tyrosine kinase that belongs to the epidermal growth factor receptor (EGFR) family. This family comprises four homologous receptors: ERFR, c-erbB-2, cerbB-3 and c-erbB-4. Activation of c-erbB-2 through autophosphorylation at tyrosine residues, is initiated by either homo- or heterodimerization and triggers intracellular signaling events. This antibody labels c-cerbB-2 that is non-phosphorylated at 1248 tyrosine residue.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies c-erbB-2 (Phospho Y1248) Rabbit Polyclonal Catalog No.

Size

Format

E18470

0.1ml

Concentrate

E18472

0.5ml

Concentrate

E18474

1.0ml

Concentrate

E18471

7.0ml

Ready-to-use

c-erbB-3/HER-3 Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide derived from the region of human c-erbB-2 that contains phosphorylated tyrosine 1248.

M3710

0.1ml

Concentrate

M3712

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human c-erbB-3.

M3714

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P

M3711

7.0ml

Ready-to-use

Species Reactivity: Human*

Clone: SP71

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Small Intestine

Control Tissue: Breast Carcinoma

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Breast Carcinoma stained with anti-c-erbB-2 (Phospho Y1248) antibody E1847

Human Small Intestine stained with anti-c-erbB-3/HER-3 antibody M371

Product Description: c-erbB-2 is a 185kDa transmembrane tyrosine kinase belongs to the epidermal growth factor receptor (EGFR) family. This family comprises four homologous receptors: ERFR, c-erbB-2, cerbB-3 and c-erbB-4. Activation of c-erbB-2 through autophosphorylation at tyrosine residues, is initiated by either homo- or heterodimerization and triggers intracellular signaling events. This antibody labels c-cerbB-2 that is non-phosphorylated at 1248 tyrosine residue.

Product Description: The gene for c-erbB-3/HER-3 is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6.2kb mRNA which is translated into a 160/180kDa glycoprotein. The c-erbB-3/HER-3 gene encodes secreted as well as transmembrane receptor tyrosine kinase due to alternative splicing. The c-erbB-3 is overexpressed in a variety of tumors including breast, stomach, pancreas, and colon.

c-erbB-2/HER-2 Rabbit Monoclonal

c-erbB-3/HER-3 Rabbit Polyclonal

Clone: SP3

Catalog No.

Size

Format

E18580

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding extracellular domain of human c-erbB-2.

E18582

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human c-erbB-3 protein.

1.0ml

Concentrate

Application: IHC-P

E18584

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E18581

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3030

0.1ml

Concentrate

M3032

0.5ml

M3034 M3031

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma, Colon Carcinoma

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Breast Carcinoma stained with anti-c-erbB-2/HER-2 antibody M303

Human Colon Carcinoma stained with anti-c-erbB-3/HER-3 antibody E1858

Product Description: c-erbB-2 is a receptor tyrosine kinase of the c-erbB family. It is closely related in structure to the epidermal growth factor receptor. c-erbB-2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas.

Product Description: The gene for c-erbB-3/HER-3 is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6.2kb mRNA which is translated into a 160/180kDa glycoprotein. The c-erbB-3/HER-3 gene encodes secreted as well as transmembrane receptor tyrosine kinase due to alternative splicing. The c-erbB-3 is overexpressed in a variety of tumors including breast, stomach, pancreas, and colon.

c-erbB-2/HER-2 Rabbit Polyclonal

c-erbB-3/HER-3 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E17930

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human c-erbB-2 protein.

E17932

0.5ml

Concentrate

Immunogen: Synthetic peptide from near N-terminus of human c-erbB-3 protein.

1.0ml

Concentrate

Application: IHC-P

E17934

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E17931

7.0ml

Ready-to-use

Catalog No.

Size

Format

E2440

0.1ml

Concentrate

E2442

0.5ml

E2444 E2441

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma, Colon Carcinoma

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane

Human Breast Carcinoma stained with anti-c-erbB-2/HER-2 antibody E244

Human Breast Carcinoma stained with anti-c-erbB-3/HER-3 antibody E1793

Product Description: c-erbB-2 is a receptor tyrosine kinase of the c-erbB family. It is closely related in structure to the epidermal growth factor receptor. c-erbB-2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas.

Product Description: The gene for c-erbB-3/HER-3 is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6.2kb mRNA which is translated into a 160/180kDa glycoprotein. The c-erbB-3/HER-3 gene encodes secreted as well as transmembrane receptor tyrosine kinase due to alternative splicing. The c-erbB-3 is overexpressed in a variety of tumors including breast, stomach, pancreas, and colon.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

37


c-erbB-4/HER-4 Rabbit Polyclonal

Chromogranin A Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

M3120

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human c-erbB-4 protein.

M3122

0.5ml

Concentrate

Immunogen: Recombinant protein encoding human chromogranin A.

1.0ml

Concentrate

Application: IHC-P, WB

M3124

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3121

7.0ml

Ready-to-use

Catalog No.

Size

Format

E2760

0.1ml

Concentrate

E2762

0.5ml

E2764 E2761

Clone: SP12

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Pancreas

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Control Lysate: A431 Molecular Weight: 140 kDa Human Breast Carcinoma stained with anti-c-erbB-4/HER-4 antibody E276

Human Pancreas stained with anti-Chromogranin A antibody M312

Product Description: c-erbB-4 or HER-4 is the fourth member of the class I receptor kinase family. HER-4 is expressed in normal skeletal muscle, heart, pituitary, cerebellum, and breast. It is specifically bound and activated by neuregulins, NRG-2, NRG-3, heparinbinding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA.

Product Description: Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It has been identified in carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

c-Fos Rabbit Polyclonal

Chromogranin A Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E1520

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human c-Fos oncoprotein.

E1522

0.5ml

Concentrate

Immunogen: Recombinant protein encoding human chromogranin A.

1.0ml

Concentrate

Application: IHC-P

E1524

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E1521

7.0ml

Ready-to-use

Catalog No.

Size

Format

E4460

0.1ml

Concentrate

E4462

0.5ml

E4464 E4461

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Skin

Control Tissue: Pancreas

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Skin stained with anti-c-Fos antibody E446

Human Pancreas stained with anti-Chromogranin A antibody E152

Product Description: The c-Fos proto-oncogene encodes a nuclear phosphoprotein and is a regulator of transcription, forming a complex with the c-Jun proto-oncogene product. Expression of the c-Fos gene is low in most normal adult tissues. However, high levels of expression have been detected in normal skin.

Product Description: Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It has been identified in carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Cholecystokinin (CCK-8) Rabbit Polyclonal

Claudin-1 Rabbit Polyclonal

Catalog No.

Size

Format

E4510

0.1ml

Concentrate

E4512

0.5ml

Concentrate

E4514

1.0ml

Concentrate

E4511

7.0ml

Ready-to-use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide sulfated cholecystokinin (26-33) amide (sulfated CCK-8).

E3410

0.1ml

Concentrate

E3412

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human claudin-1 peptide.

E3414

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P, WB

E3411

7.0ml

Ready-to-use

Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Stomach

Species Reactivity: Human*, Mouse* Isotype: Rabbit IgG Control Tissue: Breast Carcinoma, Skin

Cellular Localization: Cytoplasm

Cellular Localization: Cell Junction, Cytoplasm, Membrane

Control Lysate: Human Brain

Control Lysate: HeLa

Molecular Weight: 12 kDa

Molecular Weight: 20 kDa

Human Stomach stained with anti-Cholecystokinin (CCK-8) antibody E451

Human Skin stained with anti-Claudin-1 antibody E341

Product Description: Cholecystokinin (CCK) is a neuropeptide hormone and neurotransmitter widely distributed throughout the gastrointestinal (GI) tract and the central nervous system (CNS). Antibodies that react specifically with CCK are useful for the study of the mode of action, differential tissue expression and intracellular and subcellular localization of CCK in the CNS and periphery as well as in neuroendocrine cells of the digestive system.

38

Clone: N/A

Product Description: Claudin 1-18 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact, made up of a network of strands to act as a molecular gasket for preventing the leakage of ions and water between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different claudin composition.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Claudin-3 Rabbit Polyclonal

Claudin-7 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E10590

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of mouse claudin-3.

E10592

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human claudin-7 protein.

1.0ml

Concentrate

Application: IHC-P

E10594

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E10591

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3830

0.1ml

Concentrate

E3832

0.5ml

E3834 E3831

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Small Intestine

Control Tissue: Colon Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Membrane

Human Small Intestine stained with anti-Claudin-3 antibody E383

Human Colon Carcinoma stained with anti-Claudin-7 antibody E1059

Product Description: Claudin 1-18 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact, made up of a network of strands to act as a molecular gasket for preventing the leakage of ions and water between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different claudin composition.

Product Description: The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins, which consist of four trans-membrane domains and two extracellular loops, make up tight junction strands.

Claudin-4 Rabbit Polyclonal

Claudin-10 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E10240

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human claudin-4.

E10242

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human claudin-10 protein.

1.0ml

Concentrate

Application: IHC-P

E10244

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E10241

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3980

0.1ml

Concentrate

E3982

0.5ml

E3984 E3981

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Ovarian Carcinoma, Tonsil

Control Tissue: Brain, Skeletal Muscle

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Human Ovarian Carcinoma stained with anti-Claudin-4 antibody E398

Human Skeletal Muscle stained with anti-Claudin-10 antibody E1024

Product Description: Claudin 1-18 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact, made up of a network of strands to act as a molecular gasket for preventing the leakage of ions and water between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different claudin composition.

Product Description: The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins, which consist of four trans-membrane domains and two extracellular loops, make up tight junction strands.

Claudin-5 Rabbit Polyclonal

Claudin-11 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E8770

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of mouse claudin-5.

E8772

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human claudin-11 protein.

1.0ml

Concentrate

Application: IHC-P

E8774

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E8771

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3750

0.1ml

Concentrate

E3752

0.5ml

E3754 E3751

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung

Control Tissue: Mouse Brain, Testis

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Membrane

Human Lung stained with anti-Claudin-5 antibody E375

Human Testis stained with anti-Claudin-11 antibody E877

Product Description: Claudin 1-18 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact, made up of a network of strands to act as a molecular gasket for preventing the leakage of ions and water between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different claudin composition.

Product Description: The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudin-11 plays a major role in tight junction-specific obliteration of the intercellular space.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

39


c-Met** Rabbit Monoclonal

c-Met (Paired 1003) Rabbit Polyclonal Clone: SP44

Catalog No.

Size

Format

E18110

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human c-Met protein.

E18112

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E18114

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E18111

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3440

0.1ml

Concentrate

M3442

0.5ml

M3444 M3441

Clone: N/A Immunogen: Synthetic peptide corresponding to the region of c-Met that contains non-phosphorylated tyrosine 1003. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma Cellular Localization: Membrane

Control Tissue: Breast Carcinoma

Human Breast Carcinoma stained with anti-c-Met antibody M344

Human Breast Carcinoma stained with anti-c-Met (Paired 1003) antibody E1811

Cellular Localization: Membrane

Product Description: The c-Met oncogene was originally isolated from human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Product Description: The c-Met oncogene was identified as a transmembrane receptor-like protein with tyrosine kinase activity. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor and is involved in cell proliferation, scattering, morphogenesis, and survival. This antibody labels c-Met that is non-phosphorylated at 1003 tyrosine residue.

c-Met Rabbit Polyclonal

c-Met (Paired 1349) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18130

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human c-Met protein.

E18132

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E18134

1.0ml

Concentrate

7.0ml

Ready-to-use

Species Reactivity: Human*

E18131

7.0ml

Ready-to-use

Catalog No.

Size

Format

E10610

0.1ml

Concentrate

E10612

0.5ml

E10614 E10611

Clone: N/A Immunogen: Synthetic peptide corresponding to the region of c-Met that contains non-phosphorylated tyrosine 1349. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Ovarian Carcinoma Cellular Localization: Cytoplasm, Membrane

Control Tissue: Breast Carcinoma

Human Ovarian Carcinoma stained with anti-c-Met antibody E1061

Human Breast Carcinoma stained with anti-c-Met (Paired 1349) antibody E1813

Cellular Localization: Membrane

Product Description: The c-Met oncogene was originally isolated from human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Product Description: The c-Met oncogene was identified as a transmembrane receptor-like protein with tyrosine kinase activity. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor and is involved in cell proliferation, scattering, morphogenesis, and survival. This antibody labels c-Met that is non-phosphorylated at 1003 tyrosine residue.

c-Met (Paired 1003) Rabbit Monoclonal

c-Met (Phospho Y1003) Rabbit Monoclonal

Catalog No.

Size

Format

M3590

0.1ml

Concentrate

M3592

0.5ml

Concentrate

M3594

1.0ml

Concentrate

M3591

7.0ml

Ready-to-use

Clone: SP59

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to the region of c-Met that contains non-phosphorylated tyrosine 1003.

M3580

0.1ml

Concentrate

M3582

0.5ml

Concentrate

M3584

1.0ml

Concentrate

Application: IHC-P

M3581

7.0ml

Ready-to-use

Immunogen: Synthetic peptide corresponding to the region of c-Met that contains phosphorylated tyrosine 1003. Application: IHC-P

Species Reactivity: Human*

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Breast Carcinoma stained with anti-c-Met (Paired 1003) antibody M359

Human Breast Carcinoma stained with anti-c-Met (Phospho Y1003) antibody M358

Product Description: The c-Met oncogene was identified as a transmembrane receptor-like protein with tyrosine kinase activity. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor and is involved in cell proliferation, scattering, morphogenesis, and survival. This antibody labels c-Met that is non-phosphorylated at 1003 tyrosine residue.

40

Clone: SP58

Product Description: The c-Met oncogene was identified as a transmembrane receptor-like protein with tyrosine kinase activity. It is a cell surface receptor for hepatocyte growth factor and involves in cell proliferation, scattering, morphogenesis, and survival. This antibody is specific for Met (c-Met), only when phosphorylated at tyrosine 1003.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies c-Met (Phospho Y1003) Rabbit Polyclonal Catalog No.

Size

Format

E18100

0.1ml

Concentrate

E18102

0.5ml

Concentrate

E18104

1.0ml

Concentrate

E18101

7.0ml

Ready-to-use

Connexin-43 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to the region of c-Met that contains phosphorylated tyrosine 1003.

E2740

0.1ml

Concentrate

E2742

0.5ml

Concentrate

E2744

1.0ml

Concentrate

Application: IHC-P

E2741

7.0ml

Ready-to-use

Clone: N/A Immunogen: Synthetic peptide derived from a segment of the 3rd cytoplasmic domain of rat connexin-43. Application: IHC-P, WB

Species Reactivity: Human*

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Heart

Cellular Localization: Membrane

Cellular Localization: Cytoplasm Control Lysate: MCF7 Molecular Weight: 43 kDa

Human Breast Carcinoma stained with anti-c-Met (Phospho Y1003) antibody E1810

Human Heart stained with anti-Connexin-43 antibody E274

Product Description: The c-Met oncogene was identified as a trans-membrane receptor-like protein with tyrosine kinase activity. It is a cell surface receptor for hepatocyte growth factor and involves in cell proliferation, scattering, morphogenesis, and survival. This antibody is specific for Met (c-Met), only when phosphorylated at tyrosine 1003.

Product Description: The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexins form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Connexins are also thought to be essential for the development of labor contractions.

c-Met (Phospho Y1234/1235) Rabbit Polyclonal

Corticotropin Releasing Factor (CRF) Rabbit Polyclonal

Catalog No.

Size

Format

E18140

0.1ml

Concentrate

E18142

0.5ml

Concentrate

E18144

1.0ml

Concentrate

E18141

7.0ml

Ready-to-use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to the region of c-Met that contains phosphorylated tyrosine 1234/1235.

E10250

0.1ml

Concentrate

E10252

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human CRF protein.

E10254

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P

E10251

7.0ml

Ready-to-use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain, Placenta

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Membrane

Human Breast Carcinoma stained with anti-c-Met (Phospho Y1234/1235) antibody E1814

Human Brain stained with anti-Corticotropin Releasing Factor (CRF) antibody E1025

Product Description: The c-Met oncogene was originally isolated from human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The c-Met protein was identified as a trans-membrane receptor-like protein with tyrosine kinase activity. It is a cell surface receptor for hepatocyte growth factor and involves in cell proliferation, scattering, morphogenesis, and survival. This antibody is specific for Met (c-Met), only when phosphorylated at tyrosine 1234 and 1235.

Product Description: Corticotropin Releasing Factor (CRF), a 41 amino acids neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is critical in the regulation of the pituitary-adrenal gland axis, and in endocrine, autonomic and behavioral responses to stress. CRF is widely distributed in the brain and peripheral nervous system with the highest levels in the hypothalamic paraventricular nucleus.

c-Met (Phospho Y1349) Rabbit Polyclonal

COX-1 Rabbit Polyclonal

Catalog No.

Size

Format

E18120

0.1ml

Concentrate

E18122

0.5ml

Concentrate

E18124

1.0ml

Concentrate

E18121

7.0ml

Ready-to-use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to the region of c-Met that contains phosphorylated tyrosine 1349.

E14310

0.1ml

Concentrate

E14312

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human COX-1.

E14314

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P

E14311

7.0ml

Ready-to-use

Species Reactivity: Human*

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Esophageal Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Membrane

Human Breast Carcinoma stained with anti-c-Met (Phospho Y1349) antibody E1812

Product Description: The c-Met oncogene was originally isolated from human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The c-Met protein was identified as a trans-membrane receptor-like protein with tyrosine kinase activity. It is a cell surface receptor for hepatocyte growth factor and involves in cell proliferation, scattering, morphogenesis, and survival. This antibody is specific for Met (c-Met), only when phosphorylated at tyrosine 1349.

Clone: N/A

Human Esophageal Carcinoma stained with anti-COX-1 antibody E1431

Product Description: Cyclooxygenases 1 and 2 (COX-1, COX-2) are enzymes involved in the conversion of arichidonate to prostaglandins. COX-1 and COX-2 are very similar in structure and function, though they vary in expression. COX-1 is normally expressed in most cell types, whereas COX-2 expression is at low levels unless induced by hormonal stimuli. In some breast cancer and bladder carcinoma studies, COX-1 was expressed in the stroma cells adjacent to the tumors but not in tumor cells.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

41


COX-2 Rabbit Monoclonal

Cripto-1 Rabbit Polyclonal Clone: SP21

Catalog No.

Size

Format

E18430

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of rat COX-2.

E18432

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from human cripto-1.

1.0ml

Concentrate

Application: IHC-P

E18434

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E18431

7.0ml

Ready-to-use

Catalog No.

Size

Format

M3210

0.1ml

Concentrate

M3212

0.5ml

M3214 M3211

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Colon, Lung

Control Tissue: Colon Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Membrane

Human Breast Carcinoma stained with anti-COX-2 antibody M321

Human Colon Carcinoma stained with anti-Cripto-1 antibody E1843

Product Description: COX2 (Cyclooxygenase-2 COX2) is an inducible enzyme in response to growth factors, tumor promoters, and cytokines. COX2 is induced by a wide variety of stimuli and was initially identified as immediate-early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Product Description: The cripto gene is expressed both in embryonic stem cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers. The expression of Cripto-1 may represent a useful marker to identify cancer stem cells in melanoma, and possibly other aggressive tumors as well. Caveolin-1 can be down-regulated during the process of Cripto-1 mediated tumorigenesis in the mammary gland.

COX-2 Rabbit Polyclonal

c-TEN Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3830

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of rat COX-2.

M3832

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human c-TEN protein.

1.0ml

Concentrate

Application: IHC-P

M3834

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

M3831

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3030

0.1ml

Concentrate

E3032

0.5ml

E3034 E3031

Clone: SP83

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Colon, Lung

Control Tissue: Liver Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Membrane

Human Breast Carcinoma stained with anti-COX-2 antibody E303

Human Liver Carcinoma stained with anti-c-TEN antibody M383

Product Description: COX2 (Cyclooxygenase-2 COX2) is an inducible enzyme in response to growth factors, tumor promoters, and cytokines. COX2 is induced by a wide variety of stimuli and was initially identified as immediate-early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Product Description: c-TEN/TNS4 links the focal adhesion and cell adhesion signaling to the actin cytoskeleton. It may be involved in cell survival and migration. The protein may be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton and promote apoptosis, via its cleavage by caspase-3.

CREB-Binding Protein (CBP ) Rabbit Polyclonal

c-TEN Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E18190

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to aa 2422-2441 of human CBP.

E18192

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human c-TEN protein.

1.0ml

Concentrate

Application: IHC-P

E18194

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E18191

7.0ml

Ready-to-use

Catalog No.

Size

Format

E13840

0.1ml

Concentrate

E13842

0.5ml

E13844 E13841

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Colon Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Tonsil stained with anti-CREB-Binding Protein (CBP ) antibody E1384

Human Colon Carcinoma stained with anti-c-TEN antibody E1819

Product Description: CBP (CREB-binding protein) is a 265kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

42

Clone: SP58

Product Description: c-TEN/TNS4 links the focal adhesion and cell adhesion signaling to the actin cytoskeleton. It may be involved in cell survival and migration. The protein may be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton and promote apoptosis, via its cleavage by caspase-3.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Cullin-1 (CUL-1) Rabbit Polyclonal

Cyclin B1 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E4100

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human cullin-1.

E4102

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human cyclin B1 protein.

1.0ml

Concentrate

Application: IHC-P

E4104

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E4101

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3790

0.1ml

Concentrate

E3792

0.5ml

E3794 E3791

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma, Tonsil

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Nucleus

Human Colon Carcinoma stained with anti-Cullin-1 (CUL-1) antibody E379

Human Breast Carcinoma stained with anti-Cyclin B1 antibody E410

Product Description: Cullin-1 (CUL-1) is a member of the family of human cullin genes (cullin-1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc53 gene. Cullin-1 forms a complex with human p19skp1 and F box protein p45skp2, both of which, together with cdc53, as three subunits, form an ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The cullin-1/p19skp1/p45skp2 complex is thought to play a role in the ubiquitination of G1 regulatory proteins.

Product Description: Cyclin B1 is a regulatory protein involved in mitosis. Cyclin B1 associates with inactive p34 (cdc2) to facilitate phosphorylation of p34 (cdc2) at aa 14Thr and 15Tyr. Cyclin B1 is expressed predominantly during G2/M phase of the cell cycle. The cyclin B1-p34 (cdc2) complex plays a critical role in G2 to M transition. Cyclin B1 also contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis.

Cullin-2 (CUL-2) Rabbit Polyclonal

Cyclin D1 Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

M3040

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human cullin-2.

M3042

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human cyclin D1.

1.0ml

Concentrate

Application: IHC-P

M3044

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

M3041

7.0ml

Ready-to-use

Catalog No.

Size

Format

E4160

0.1ml

Concentrate

E4162

0.5ml

E4164 E4161

Clone: SP4

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Mantle Cell Lymphoma, Tonsil

Cellular Localization: Nucleus

Cellular Localization: Nucleus Control Lysate: MCF7 Molecular Weight: 36 kDa

Human Tonsil stained with anti-Cullin-2 (CUL-2) antibody E416

Human Mantle Cell Lymphoma stained with anti-Cyclin D1 antibody M304

Product Description: Cullin-2 (CUL-2) is a member of human cullin gene family (CUL-1, -2, -3, -4a, -4b, and -5). It is the core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes. The ECS ligase complexes mediate the ubiquitination of target proteins such as hypoxia-inducible factor (HIF).

Product Description: Cyclin D1 or PRAD-1 or BCL-1 is one of the key cell cycle regulators, and functions in association with CDK4 and / or CDK6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).

CXCR4 Rabbit Polyclonal

Cyclin D1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E1540

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from the human CXCR4.

E1542

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human cyclin D1.

1.0ml

Concentrate

Application: IHC-P

E1544

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E1541

7.0ml

Ready-to-use

Catalog No.

Size

Format

E18440

0.1ml

Concentrate

E18442

0.5ml

E18444 E18441

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Mantle Cell Lymphoma, Tonsil

Cellular Localization: Membrane

Cellular Localization: Nucleus Control Lysate: MCF7 Molecular Weight: 36 kDa

Human Placenta stained with anti-CXCR4 antibody E1844

Product Description: CXCR4 is a principal coreceptor for T cell tropic strains of HIV1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV1 and mediates CD4 independent infection by HIV2. The chemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4 is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells.

Human Mantle Cell Lymphoma stained with anti-Cyclin D1 antibody E154

Product Description: Cyclin D1 or PRAD-1 or BCL-1 is one of the key cell cycle regulators, and functions in association with CDK4 and / or CDK6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

43


Cyclin E Rabbit Polyclonal

D4-GDI Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2430

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human cyclin E.

E2432

0.5ml

Concentrate

Immunogen: Recombinant full-length human D4-GDI protein.

1.0ml

Concentrate

Application: IHC-P

E2434

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-use

Species Reactivity: Human*

E2431

7.0ml

Ready-to-use

Catalog No.

Size

Format

E3600

0.1ml

Concentrate

E3602

0.5ml

E3604 E3601

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Placenta

Control Tissue: Placenta, Tonsil

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Nucleus Control Lysate: Jurkat Molecular Weight: 23 kDa

Human Breast Carcinoma stained with anti-Cyclin E antibody E360

Human Placenta stained with anti-D4-GDI antibody E243

Product Description: Cyclin E is a regulatory subunit of CDK-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of Cyclin E. In vitro analysis indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1.

Product Description: D4-GDI belongs to the family of Rho-GDIs or GDP-dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI-1, D4-GDI/ RhoGDI-2 and RhoGDI-3. It inhibits the release of GDP from the Rho proteins, essential for GTP-loading and activation of GTPase activity. D4- GDI is located in the cytoplasm and is involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins.

CYR61 Rabbit Polyclonal

D5DR Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E12580

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human CYR61.

E12582

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 459-477 of human D5DR.

1.0ml

Concentrate

Application: IHC-P

E12584

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E12581

7.0ml

Ready-to-use

Catalog No.

Size

Format

E18300

0.1ml

Concentrate

E18302

0.5ml

E18304 E18301

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Placenta

Control Tissue: Brain

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Colon stained with anti-CYR61 antibody E1830

Human Brain stained with anti-D5DR antibody E1258

Product Description: CYR61 is a secreted cysteine-rich heparin binding protein, encoded by a growth factor inducible immediate-early gene. CYR61 is a member of a distinct family of angiogenic and vasculogenic regulators designated CCN proteins. As an angiogenic inducer, CYR61 binds to the cell surface receptor to promotes DNA synthesis of human vascular endothelial cells. CYR61 is also detected in a wide variety of tumors as it induces tumor growth.

Product Description: The dopamine receptors are divided into two classes, D1 and D2, which differ in their functional characteristics. D1DR and D5DR compose the D1 class of dopamine receptors, and D2DR, D3DR and D4DR compose the D2 class. D1 receptors stimulate adenylyl cyclase, while D2 receptors inhibit adenylyl cyclase activity.

Cytochrome C Rabbit Polyclonal

DAPK2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E16860

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant full length human cytochrome C protein.

E16862

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human DAPK2 protein.

1.0ml

Concentrate

Application: IHC-P

E16864

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-use

Species Reactivity: Human*

E16861

7.0ml

Ready-to-use

Catalog No.

Size

Format

E12410

0.1ml

Concentrate

E12412

0.5ml

E12414 E12411

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Brain stained with anti-Cytochrome C antibody E1241

Human Breast Carcinoma stained with anti-DAPK2 antibody E1686

Product Description: Cytochrome C is an electron transporting protein that resides within the inter-membrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. Cytocrome C is up-regulated and it is rapidly released from the mitochondria into the cytosol in apoptotic processes. Cytosolic cytochrome C participates in the activation of apoptotic effectors such as caspase-3 and ICE family molecule.

44

Clone: N/A

Product Description: Death-associated protein kinase 2 (DAPK2) is a protein belonging to the serine/threonine protein kinase family. It is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFN gamma induced apoptosis. The messenger RNA of DAPK2 is expressed in multiple human tissues.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Desmin Rabbit Polyclonal

DJ-1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E8760

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide mapping near the C-terminus of human desmin.

E8762

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human DJ-1 protein.

1.0ml

Concentrate

Application: IHC-P

E8764

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E8761

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2570

0.1ml

Concentrate

E2572

0.5ml

E2574 E2571

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Sarcoma, Skeletal Muscle

Control Tissue: Breast

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Nucleus Control Lysate: Jurkat Molecular Weight: 22 kDa

Human Skeletal Muscle stained with anti-Desmin antibody E257

Human Breast stained with anti-DJ-1 antibody E876

Product Description: Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance.

Product Description: DJ-1 protein has 189 amino acids and was cloned from a HeLa cell cDNA library as a novel oncogene which weakly transformed NIH3T3 cells. The expression of DJ-1 is induced by growth signals and is translocated from the cytoplasm to the nucleus during the S phase, although no nuclear localization signals are present. It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved in Ras-related signal transduction pathway.

DFF-45 Rabbit Polyclonal

DOG-1 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3310

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human DFF-45 protein.

M3312

0.5ml

Concentrate

Immunogen: Synthetic peptide of human DOG-1 protein.

1.0ml

Concentrate

Application: IHC-P

M3314

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3311

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3900

0.1ml

Concentrate

E3902

0.5ml

E3904 E3901

Clone: SP31

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Gastrointestinal Stromal Tumor

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Colon Carcinoma stained with anti-DFF-45 antibody E390

Human Gastrointestinal Stromal Tumor stained with anti-DOG-1 antibody M331

Product Description: DFF45 and DFF40 (ICAD and CAD) are two subunits of heterodimeric caspase activated DNAse. DFF45/ ICAD (Inhibitor of Caspase Activated DNAse) acts as a chaperon for appropriate folding of DFF40 and it also acts as inhibitor of DFF40. The activation of caspase 3 cleaves DFF to release DFF40/CAD, which oligomerizes into a large active complex and eventually causes the degradation of DNA in nuclei. Hence cleavage of DFF45 is an important step in apoptosis.

Product Description: DOG-1, a novel marker, is expressed ubiquitously in gastrointestinal stromal tumors (GIST) irrespective of KIT or PDGFR alpha mutation status.

DHFR Rabbit Polyclonal

DOG-1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E16710

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human DHFR.

E16712

0.5ml

Concentrate

Immunogen: Synthetic peptide of human DOG-1 protein.

1.0ml

Concentrate

Application: IHC-P

E16714

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E16711

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18220

0.1ml

Concentrate

E18222

0.5ml

E18224 E18221

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Gastrointestinal Stromal Tumor

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Breast Carcinoma stained with anti-DHFR antibody E1822

Human Gastrointestinal Stromal Tumor stained with anti-DOG-1 antibody E1671

Product Description: Dihydrofolate reductase (DHFR) converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Product Description: DOG-1, a novel marker, is expressed ubiquitously in gastrointestinal stromal tumors (GIST) irrespective of KIT or PDGFR alpha mutation status.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

45


DOK-1 Rabbit Polyclonal

Dysferlin Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2670

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human DOK-1 protein.

E2672

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human dysferlin.

1.0ml

Concentrate

Application: IHC-P

E2674

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2671

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16830

0.1ml

Concentrate

E16832

0.5ml

E16834 E16831

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Skeletal Muscle

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane Control Lysate: Human Skeletal Muscle Molecular Weight: ~231 kDa

Human Breast Carcinoma stained with anti-DOK-1 antibody E1683

Human Skeletal Muscle stained with anti-Dysferlin antibody E267

Product Description: DOK-1 (P62dok, Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK-1 is a major substrate for many tyrosine kinases including c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation by kinases, DOK-1 forms a complex with ras GTPase-activating protein.

Product Description: Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle muscular dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation.

DOK-2 Rabbit Polyclonal

Dystrophin Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2660

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human DOK-2 protein.

E2662

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human dystrophin.

1.0ml

Concentrate

Application: IHC-P

E2664

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2661

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16730

0.1ml

Concentrate

E16732

0.5ml

E16734 E16731

Clone: N/A

Species Reactivity: Human*, Mouse*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Skeletal Muscle

Cellular Localization: Cytoplasm

Cellular Localization: Membrane Control Lysate: Human Skeletal Muscle Molecular Weight: 427 kDa

Human Breast Carcinoma stained with anti-DOK-2 antibody E1673

Human Skeletal Muscle stained with anti-Dystrophin antibody E266

Product Description: Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. DOK-2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. DOK-2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung.

Product Description: Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne’s muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation. This antibody is highly specific to dystrophin and shows no cross-reaction with C-protein (an isoform of α-actinin), α-actin, or human muscle spectrin.

DRAK2 Rabbit Polyclonal

Dystrophin Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E14010

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human DRAK2 protein.

E14012

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human dystrophin.

1.0ml

Concentrate

Application: IHC-P

E14014

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E14011

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16750

0.1ml

Concentrate

E16752

0.5ml

E16754 E16751

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgG1/k

Control Tissue: Breast Carcinoma

Control Tissue: Skeletal Muscle

Cellular Localization: Nucleus

Cellular Localization: Membrane

Human Breast Carcinoma stained with anti-DRAK2 antibody E1675

Human Skeletal Muscle stained with anti-Dystrophin antibody E1401

Product Description: Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosisinducing protein kinases). DRAKs contain a N-terminal kinase domain and a C-terminus regulation domain. Overexpression of DRAK2 induces apoptosis. DRAK2 is located in nucleus and its mRNA is ubiquitously expressed in human tissue.

46

Clone: SPM499

Product Description: Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne’s muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation. This antibody is highly specific to dystrophin and shows no cross-reaction with C-protein (an isoform of α-actinin), α-actin, or human muscle spectrin.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies E2F-2 Rabbit Polyclonal

Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal

Catalog No.

Size

Format

E13610

0.1ml

Concentrate

E13612

0.5ml

Concentrate

E13614

1.0ml

Concentrate

N/A

N/A

N/A

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to N-terminus of human E2F-2.

M3840

0.1ml

Concentrate

M3842

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human EGFR protein.

M3844

1.0ml

Concentrate

Application: WB

Application: IHC-P, WB

M3841

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: SP84

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG Control Tissue: N/A

Control Tissue: Lung Squamous Cell Carcinoma

Cellular Localization: N/A

Cellular Localization: Membrane

Control Lysate: HepG2

Control Lysate: A431

Molecular Weight: 48 kDa

Molecular Weight: 175 kDa

E1361

Human Lung Squamous Cell Carcinoma stained with anti-Epidermal Growth Factor Receptor (EGFR) antibody M384

Product Description: E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-2 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.

Product Description: EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HBEGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

EGFL7 Rabbit Polyclonal

Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3090

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to an internal region of human EGFL7 protein.

M3092

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human EGFR protein.

1.0ml

Concentrate

Application: IHC-P

M3094

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3091

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E19130

0.1ml

Concentrate

E19132

0.5ml

E19134 E19131

Clone: SP9

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Lung, Placenta

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Membrane

Human Placenta stained with anti-EGFL7 antibody E1913

Human Placenta stained with anti-Epidermal Growth Factor Receptor (EGFR) antibody M309

Product Description: Epidermal growth factor-like protein 7 (EGFL7), also known as VEstatin, MEGF7, Notch4-like protein or Zneu1, is a 29.6 kDa protein produced and secreted by endothelium. The protein is also expressed by some normal and malignant tissues. The main function of EGFL7 is to regulate angiogenesis and vasculogenesis. A high level of EFGL-7 can be found in tissues with active angiogenesis of a variety of disorders.

Product Description: EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HBEGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Ephrin A4 Rabbit Polyclonal

Epidermal Growth Factor Receptor (EGFR) Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E6690

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant full length human ephrin A4 protein.

E6692

0.5ml

Concentrate

Immunogen: Extracellular domain of human recombinant EGFR protein.

1.0ml

Concentrate

Application: IHC-P

E6694

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E6691

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E11150

0.1ml

Concentrate

E11152

0.5ml

E11154 E11151

Clone: SPM341

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgG1

Control Tissue: Brain Cellular Localization: Cytoplasm, Membrane

Control Tissue: Placenta, Skin, Squamous Cell Carcinoma

Human Brain stained with anti-Ephrin A4 antibody E1115

Human Placenta stained with anti-Epidermal Growth Factor Receptor (EGFR) antibody E669

Product Description: Ephrin-A4, also known as LERK-4 or ELF-4, is a ligand for the erythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases and it binds EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. Ephrin-A4 interacts with activated B lymphocytes and dendritic cells and it is expressed in the adult spleen, lymph node, prostate, ovary, small intestine, and colon, and in fetal heart, lung, liver and kidney.

Cellular Localization: Membrane

Product Description: EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HBEGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

47


Epidermal Growth Factor Receptor (EGFR) Rabbit Polyclonal

ERCC1 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E18890

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human EGFR protein.

E18892

0.5ml

Concentrate

Immunogen: Recombinant human ERCC1 protein.

1.0ml

Concentrate

Application: IHC-P, WB

E18894

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18891

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18170

0.1ml

Concentrate

E18172

0.5ml

E18174 E18171

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Esophageal Carcinoma, Placenta

Control Tissue: Lung Squamous Cell Carcinoma, Rectal Carcinoma

Cellular Localization: Membrane

Cellular Localization: Nucleus

Control Lysate: A431

Control Lysate: HeLa

Molecular Weight: 170 kDa

Molecular Weight: 39 kDa

Human Esophageal Carcinoma stained with antiEpidermal Growth Factor Receptor (EGFR) antibody E1817

Human Lung Squamous Cell Carcinoma stained with anti-ERCC1 antibody E1889

Product Description: EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HBEGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Description: The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to S. cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. The NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3’ of the DNA lesion while the ERCC1-XPF complex makes the 5’ incision.

ERAB Rabbit Polyclonal

ERCC1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18550

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human ERAB protein.

E18552

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human ERCC1 protein.

1.0ml

Concentrate

Application: IHC-P

E18554

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18551

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E10320

0.1ml

Concentrate

E10322

0.5ml

E10324 E10321

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Alzheimer’s Brain, Tonsil

Control Tissue: Colon

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Alzheimer’s Brain stained with anti-ERAB antibody E1032

Human Colon stained with anti-ERCC1 antibody E1855

Product Description: ERAB also known as 3-hydroxyacyl-CoA dehydrogenase type-2 belongs to the short-chain dehydrogenases/reductases (SDR) family. It is one of the mutated genes associated with early onset Alzheimer’s disease. It is shown to be expressed at high levels in Alzheimer’s disease-affected brain and may be related to neuronal dysfunction in Alzheimer’s disease.

Product Description: The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to S. cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. The NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3’ of the DNA lesion while the ERCC1-XPF complex makes the 5’ incision.

ERCC1 Rabbit Monoclonal

ERK-1 Rabbit Polyclonal Clone: SP68

Catalog No.

Size

Format

Concentrate

Immunogen: Recombinant human ERCC1 protein.

E3560

0.1ml

Concentrate

Concentrate

Application: IHC-P

E3562

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human ERK-1 protein.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-Use

Species Reactivity: Human*

E3564

7.0ml

E3561

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3680

0.1ml

M3682

0.5ml

M3684 M3681

Isotype: Rabbit IgG Control Tissue: Colon, Non-Small Cell Lung Carcinoma

Species Reactivity: Human* Isotype: Rabbit IgG

Cellular Localization: Nucleus

Control Tissue: Breast Carcinoma

Human Lung Carcinoma stained with anti-ERCC1 antibody M368

Human Breast Carcinoma stained with anti-ERK-1 antibody E356

Product Description: The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to S. cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. The NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3’ of the DNA lesion while the ERCC1-XPF complex makes the 5’ incision.

48

Clone: N/A

Cellular Localization: Cytoplasm

Product Description: ERK-1/extracellular signal-regulated kinase 1/p44 MAP kinase/ microtubule-associated protein 2 kinase/myelin basic protein kinase/EGF receptor T669 kinase is ubiquitously expressed in various tissues and cells. It gets activated by phosphorylation of neighboring serine and threonine residues by MEK-1 and MEK-2 in response to diverse stimuli like growth factors and mitogens.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies ERK-2 Rabbit Polyclonal

Estrogen Receptor (ER) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E1640

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of mouse ERK-2 protein.

E1642

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human estrogen receptor.

1.0ml

Concentrate

Application: IHC-P

E1644

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E1641

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3970

0.1ml

Concentrate

E3972

0.5ml

E3974 E3971

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Nucleus

Human Breast Carcinoma stained with anti-ERK-2 antibody E397

Human Breast Carcinoma stained with anti-Estrogen Receptor (ER) antibody E164

Product Description: Extracellular signal-regulated kinase 2 (ERK-2) is a serine-threonine kinase phosphorylates microtubule associated protein-2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.

Product Description: This antibody recognizes a protein of 67kDa, which is identified as estrogen receptor (ER). This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland.

ESA/Ep-CAM Mouse Monoclonal

EZH2 Rabbit Polyclonal

Clone: SPM491

Catalog No.

Size

Format

E19090

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant human ESA protein expressed in E. coli.

E19092

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human EZH2 protein.

1.0ml

Concentrate

Application: IHC-P

E19094

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E19091

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E11170

0.1ml

Concentrate

E11172

0.5ml

E11174 E11171

Clone: N/A

Species Reactivity: Human*

Isotype: Mouse IgG1/k

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Prostate Carcinoma

Cellular Localization: Membrane

Cellular Localization: Nucleus

Human Breast Carcinoma stained with anti-ESA/Ep-CAM antibody E1117

Human Prostate Carcinoma stained with anti-EZH2 antibody E1909

Product Description: EGP40 is a 40kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Product Description: KMT6 / Enhancer of zeste homologue 2 (EZH2) is a histone lysine methyl transferase which is associated with transcriptional repression. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. The expression of EZH2 is highly expressed in follicular T cells and a variety of tumors including lymphoma, breast and prostate cancer.

Estrogen Receptor (ER) Rabbit Monoclonal

F4/80 Rabbit Monoclonal

Catalog No.

Size

Format

M3010

0.1ml

Concentrate

M3012

0.5ml

Concentrate

M3014

1.0ml

Concentrate

M3011

7.0ml

Ready-to-Use

Clone: SP1

Catalog No.

Size

Format

Immunogen: Synthetic peptide derived from C-terminus of human estrogen receptor alpha.

M4150

0.1ml

Concentrate

M4152

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of mouse F4/80 protein.

M4154

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

M4151

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: SP115

Species Reactivity: Mouse* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Mouse Liver

Control Tissue: Breast Carcinoma

Cellular Localization: Membrane

Cellular Localization: Nucleus Control Lysate: MCF7 Molecular Weight: 67 kDa Human Breast Carcinoma stained with anti-Estrogen Receptor (ER) antibody M301

Product Description: This antibody recognizes a protein of 67kDa, which is identified as estrogen receptor (ER). This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland.

Mouse Liver stained with anti-F4/80 antibody M415

Product Description: F4/80 is a cell surface glycoprotein and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages and a subpopulation of dendritic cells. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ regulatory T cells.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

49


F4/80 Rabbit Polyclonal

FcRH5 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E19250

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of mouse F4/80 protein.

E19252

0.5ml

Concentrate

Immunogen: Synthetic peptide near the C-terminus of human FcRH5 protein.

1.0ml

Concentrate

Application: IHC-P

E19254

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E19251

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18970

0.1ml

Concentrate

E18972

0.5ml

E18974 E18971

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung

Control Tissue: Tonsil

Cellular Localization: Membrane

Cellular Localization: Membrane

Human Lung stained with anti-F4/80 antibody E1897

Human Tonsil stained with anti-FcRH5 antibody E1925

Product Description: F4/80 antigen is a 160kDa cell surface glycoprotein expressed in mature tissue macrophages such as Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, and bone marrow stroma. F4/80 is also expressed in a subpopulation of dendritic cells but absent from macrophages located in T cell areas of the spleen and lymph node. Recent studies suggest a role for F4/80 in the generation of CD8+ regulatory T cells.

Product Description: Fc receptor homologs (FcRH5), also known as IRTA2 proteins, are transmembrane proteins with sequence homology to classical Fc receptor families. FcRH5 function as activating or inhibitory receptors on discrete B cell populations and are involved in B cell development and differentiation in peripheral lymphoid organs. FcRH5 expression is deregulated in multiple myeloma and Burkitt lymphoma cell lines with 1q21 abnormalities.

FAS Ligand Rabbit Polyclonal

FGFR-1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E10580

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human FAS ligand.

E10582

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human FGFR-1 protein.

1.0ml

Concentrate

Application: IHC-P

E10584

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E10581

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2710

0.1ml

Concentrate

E2712

0.5ml

E2714 E2711

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate, Testis, Tonsil

Control Tissue: Breast Carcinoma, Pituitary

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Membrane

Human Tonsil stained with anti-FAS Ligand antibody E271

Human Breast Carcinoma stained with anti-FGFR-1 antibody E1058

Product Description: Fas ligand is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express Fas, where Fas ligand is expressed predominantly on activated T cells. Fas and Fas ligand are involved in the down-regulation of immune reactions as well as T cellmediated cytotoxicity.

Product Description: Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides that regulates growth and differentiation in most cells. FGFR-1 is one of the four genes encoding for high affinity cell surface FGF receptors (FGFRs). FGFRs are members of the tyrosine kinase family of growth factor receptors. At the mRNA level, FGFR-1 is highly expressed in developing human tissues such as brain, vascular basement membrane, and skin.

FAS-Associated Phosphatase (FAP-1) Rabbit Polyclonal

FGFR-2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E10570

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 22902491 of human FAP-1.

E10572

0.5ml

Concentrate

Immunogen: Synthetic peptide from aa 362-374 of human FGFR-2 protein.

1.0ml

Concentrate

Application: IHC-P

E10574

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E10571

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E12360

0.1ml

Concentrate

E12362

0.5ml

E12364 E12361

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma, Placenta

Control Tissue: Breast Carcinoma, Liver Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Colon Carcinoma stained with anti-FAS-Associated Phosphatase (FAP-1) antibody E1236

Human Breast Carcinoma stained with anti-FGFR-2 antibody E1057

Product Description: FAS-associated phosphatase (FAP-1), a novel protein tyrosine phosphatase, regulates FAS-induced apoptosis and nerve growth factor receptor (NGFR)-mediated pro-apoptotic signaling. It has been shown to associate with the fifteen amino acids at the carboxy terminus of FAS. FAP-1 contains three isoforms and a membrane-binding domain. It is present in most tissues with the exception of the liver and skeletal muscle, and is most abundant in lung, kidney and fetal brain.

50

Clone: N/A

Product Description: Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides that regulates growth and differentiation in most cells. FGFR-2 is one of the four genes encoding for high affinity cell surface FGF receptors (FGFRs). FGFRs are members of the tyrosine kinase family of growth factor receptors. FGFR-2 is highly expressed in developing human tissues such as brain, choroids plexus, and lung.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies FGFR-3 Rabbit Polyclonal

FLK-1/KDR/VEGFR-2 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3710

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human FGFR-3 protein.

E3712

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human FLK-1.

1.0ml

Concentrate

Application: IHC-P

E3714

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3711

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E10230

0.1ml

Concentrate

E10232

0.5ml

E10234 E10231

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast, Breast Carcinoma

Control Tissue: Angiosarcoma, Placenta

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Breast stained with anti-FGFR-3 antibody E1023

Human Placenta stained with anti-FLK-1/KDR/VEGFR-2 antibody E371

Product Description: Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides that regulates growth and differentiation in most cells. FGFR-3 is one of the four genes encoding for high affinity cell surface FGF receptors (FGFRs). FGFRs are members of the tyrosine kinase family of growth factor receptors. FGFR-3 is widely expressed in many fetal and adult human and animal tissues.

Product Description: FLK-1/KDR/VEGFR2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to FLT-1 and FLT-4.

FLI-1 Rabbit Polyclonal

FLT-1/VEGFR1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2800

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human FLI-1.

E2802

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human FLT-1.

1.0ml

Concentrate

Application: IHC-P

E2804

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2801

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E4280

0.1ml

Concentrate

E4282

0.5ml

E4284 E4281

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Angiosarcoma, Ewing Sarcoma

Control Tissue: Angiosarcoma, Placenta

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Ewing Sarcoma stained with anti-FLI-1 antibody E428

Human Placenta stained with anti-FLT-1/VEGFR1 antibody E280

Product Description: Fli-1 is a member of the ETS-1gene family which includes ETS-1, ETS-2, ERG-1, ERG-2, ELK, E74, FLI-1, PU-1 and PEA-3. Fli-1 is a nuclear protein and its defects are the cause of Ewing sarcoma (ES), a highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue in children and adolescents.

Product Description: FLT-1/vascular endothelial growth factor receptor 1 (VEGFR1), a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to FLK-1 and FLT-4.

FLK-1 (mouse) Rabbit Monoclonal

FLT-4/VEGFR3 Rabbit Polyclonal

Clone: SP123

Catalog No.

Size

Format

E3870

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide near the N-terminus of mouse FLK-1 protein.

E3872

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human FLT-4 protein.

1.0ml

Concentrate

Application: IHC-P

E3874

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3871

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M4230

0.1ml

Concentrate

M4232

0.5ml

M4234 M4231

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Breast Carcinoma, Placenta

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane

Human Placenta stained with anti-FLK-1 (mouse) antibody M423

Human Placenta stained with anti-FLT-4/VEGFR3 antibody E387

Product Description: FLK-1/KDR/VEGFR2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to FLT-1 and FLT-4.

Product Description: Three cell membrane receptor tyrosine kinases, FLT (also designated VEGF-R1), FLK-1 (also designated VEGF-R2) and FLT-4 (also designated VEGF-R3), are putatively involved in the growth of endothelial cells as characterized by the presence of seven immuno-globulin-like sequences in their extracellular domain. On the basis of structural similarity to FLT and FLK-1, it has been speculated that FLT-4 might represent a third receptor for either VEGF or a VEGF-related ligand.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

51


Fn14 Rabbit Polyclonal

FOXO1A Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Internal region of human Fn14.

E17820

0.1ml

Concentrate

Concentrate

Application: IHC-P

E17822

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human FOXO1A.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-Use

Species Reactivity: Human*

E17824

7.0ml

E17821

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E19080

0.1ml

E19082

0.5ml

E19084 E19081

Isotype: Rabbit IgG

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: Kidney, Placenta Cellular Localization: Membrane

Control Tissue: Prostate Carcinoma

Human Placenta stained with anti-Fn14 antibody E1908

Human Prostate Carcinoma stained with anti-FOXO1A antibody E1782

Cellular Localization: Cytoplasm, Nucleus

Product Description: Fibroblast growth factor-inducible 14 (Fn14) is the receptor for Tweak, a member of the TNF receptor family. It was first described as a weak inducer of apoptosis in some cell types. It is thought to promote angiogenesis and the proliferation of endothelial cells as well as modulating cellular adhesion to matrix proteins. Fn14 is highly expressed in glioma and some cancer cases of breast, pancreas, and liver.

Product Description: FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a member of a subfamily of the forkhead homeotic gene family of transcription factors. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C-terminus of the FOXO1A protein. Chromosomal aberrations involving FOXO1A are associated with rhabdomyosarcoma 2 (RMS2) or alveolar rhabdomyosarcoma.

FOXA1 Rabbit Monoclonal

FOXO3A Rabbit Polyclonal Clone: SP88

Catalog No.

Size

Format

E17730

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human FOXA1 protein.

E17732

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human FOXO3A protein.

1.0ml

Concentrate

Application: IHC-P

E17734

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17731

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3880

0.1ml

Concentrate

M3882

0.5ml

M3884 M3881

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate

Control Tissue: Breast Carcinoma, Tonsil

Cellular Localization: Nucleus in Breast Carcinoma, Prostate. Cytoplasm in most malignancies.

Cellular Localization: Cytoplasm, Nucleus

Human Prostate stained with anti-FOXA1 antibody M388

Human Breast Carcinoma stained with anti-FOXO3A antibody E1773

Product Description: This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Product Description: FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

FOXA1 Rabbit Polyclonal

FOXP1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E19060

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human FOXA1 protein.

E19062

0.5ml

Concentrate

Immunogen: Synthetic peptide near C-terminus of human FOXP1.

1.0ml

Concentrate

Application: IHC-P

E19064

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E19061

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18850

0.1ml

Concentrate

E18852

0.5ml

E18854 E18851

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Nucleus

Human Breast Carcinoma stained with anti-FOXA1 antibody E1885

Human Tonsil stained with anti-FOXP1 antibody E1906

Product Description: This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

52

Clone: N/A

Product Description: Forkhead box (FOX) proteins are a group of 43 conserved transcription factors that are further divided into subgroups. The FOXP subgroup has four members, FOXP1 to FOXP4, which are widely expressed and contribute some critical roles in organ development, immune response, and cancer pathogenesis. FOXP1 is a transcriptional repressor that plays an important role in lung, cardiac valve, and B cell development.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies FOXP3 Rabbit Monoclonal

FPGS Rabbit Polyclonal Clone: SP97

Catalog No.

Size

Format

E18340

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human FOXP3 protein.

E18342

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human FPGS.

1.0ml

Concentrate

Application: IHC-P

E18344

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18341

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3970

0.1ml

Concentrate

M3972

0.5ml

M3974 M3971

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Breast Carcinoma, Liver Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Tonsil stained with anti-FOXP3 antibody M397

Human Breast Carcinoma stained with anti-FPGS antibody E1834

Product Description: Forkhead box protein P3 (FOXP3) is a member of the forkhead/ winged-helix family of transcriptional regulators. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25+ and CD4+ T cells, at low level in CD4+/CD25- cells, and is absent in CD4-/CD8+ T cells. FOXP-3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Product Description: Folylpoly-gamma-glutamate synthetase (FPGS) converts folates to polyglutamate derivatives by catalyzing the addition of several equivalents of glutamic acid to the gamma-carboxyl group in the side chain of folate cofactors and analogs. FFPGS is widely expressed in human tumors and is tightly linked either to proliferation or to a lack of differentiation.

FOXP3 Rabbit Polyclonal

FRA-2 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E4210

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human FOXP3 protein.

E4212

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human FRA-2 protein.

1.0ml

Concentrate

Application: IHC-P

E4214

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4211

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18490

0.1ml

Concentrate

E18492

0.5ml

E18494 E18491

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Prostate Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Tonsil stained with anti-FOXP3 antibody E1849

Human Prostate Carcinoma stained with anti-FRA-2 antibody E421

Product Description: Forkhead box protein P3 (FOXP3) is a member of the forkhead/ winged-helix family of transcriptional regulators. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25+ and CD4+ T cells, at low level in CD4+/CD25- cells, and is absent in CD4-/CD8+ T cells. FOXP-3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Product Description: FRA-2 has homologous sequence with c-FOS, FRA-1, and FOS B. It forms heterodimers with c-Jun that recognize specific DNA sequences, such as the binding site for transcription factor AP-1.

FOXP4 Rabbit Polyclonal

Fragile Histidine Triad (FHIT) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3640

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human FOXP4 protein.

E3642

0.5ml

Concentrate

Immunogen: Recombinant full length human FHIT protein.

1.0ml

Concentrate

Application: IHC-P

E3644

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3641

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16390

0.1ml

Concentrate

E16392

0.5ml

E16394 E16391

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung

Control Tissue: Breast

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Lung stained with anti-FOXP4 antibody E1639

Human Breast stained with anti-Fragile Histidine Triad (FHIT) antibody E364

Product Description: FOXP4 gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Many members of the forkhead box gene family, including members of subfamily P, have roles in mammalian oncogenesis. This gene may play a role in the development of tumors of the kidney and larynx.

Product Description: This antibody reacts with human FHIT (Fragile Histidine Triad). Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach, and pancreas. In normal cells, FHIT may act as a tumor suppressor.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

53


GADD153 Rabbit Polyclonal

Gastrin-1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E4490

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant human full length GADD153 protein.

E4492

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of gastrin-1.

1.0ml

Concentrate

Application: IHC-P

E4494

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4491

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E10980

0.1ml

Concentrate

E10982

0.5ml

E10984 E10981

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Stomach

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-GADD153 antibody E1098

Human Stomach stained with anti-Gastrin-1 antibody E449

Product Description: GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. GADD153 expression is induced by a variety of cellular stresses. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from classical C/EBP binding sites.

Product Description: Gastrin is a 17 amino acid peptide hormone and neurotransmitter widely distributed throughout the gastrointestinal (GI) tract and the central nervous system (CNS). Antibodies that react specifically with gastrin may be used to study the differential tissue expression and intracellular and subcellular localization of gastrin in neuroendocrine cells of the gastrointestinal tract, and in the CNS.

Gamma Glutamylcysteine Synthetase (GCS) Rabbit Polyclonal

GDNF Rabbit Polyclonal

Catalog No.

Size

Format

E16740

0.1ml

Concentrate

E16742

0.5ml

Concentrate

E16744

1.0ml

Concentrate

E16741

7.0ml

Ready-to-Use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to internal region of rat heavy subunit of GCS protein.

E11020

0.1ml

Concentrate

E11022

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 186-205 of human GDNF.

E11024

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

E11021

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain (Hippocampus)

Control Tissue: Kidney, Testis

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm Control Lysate: Human Kidney Molecular Weight: 73 kDa Human Testis stained with anti-Gamma Glutamylcysteine Synthetase (GCS) antibody E1674

Human Brain stained with anti-GDNF antibody E1102

Product Description: Gamma Glutamylcysteune Synthetase (GCS) is the first enzyme and rate limiting step in the glutathione biosynthesis pathway. Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. It is composed of heavy and light subunits. These subunits are coded by different genes which are controlled by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH.

Product Description: Glial cell line-derived neurotrophic factor (GDNF) has been identified as a potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons. GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. It was suggested that GDNF may have utility in the treatment of Parkinson’s disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.

GANP Rabbit Polyclonal

Glial Fibrillary Acidic Protein (GFAP) Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3780

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 1-150 of human GANP.

M3782

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human GFAP protein.

1.0ml

Concentrate

Application: IHC-P

M3784

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3781

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E14080

0.1ml

Concentrate

E14082

0.5ml

E14084 E14081

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Astrocytoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Tonsil stained with anti-GANP antibody E1408

Human Astrocytoma stained with anti-Glial Fibrillary Acidic Protein (GFAP) antibody M378

Product Description: Germinal center-associated nuclear protein (GANP) is thought to be involved in the nuclear localization pathway of MCM3 and in regulation of cell proliferation of antigen-driven B cells in germinal centers. It carries an MCM-binding domain in the carboxyl-terminal side and it is up-regulated selectively in germinal centers.

54

Clone: SP78

Product Description: Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system. The antibody to GFAP is useful for documenting astrocytic differentiation in tumors outside the CNS.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Glial Fibrillary Acidic Protein (GFAP) Rabbit Polyclonal

GluR1 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E3060

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human GFAP.

E3062

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human GluR1.

1.0ml

Concentrate

Application: IHC-P

E3064

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3061

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18320

0.1ml

Concentrate

E18322

0.5ml

E18324 E18321

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Astrocytoma

Control Tissue: Brain

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Astrocytoma stained with anti-Glial Fibrillary Acidic Protein (GFAP) antibody E1832

Human Brain stained with anti-GluR1 antibody E306

Product Description: Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system. The antibody to GFAP is useful for documenting astrocytic differentiation in tumors outside the CNS.

Product Description: Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1-7, NMDA1-3, KAI-2), which comprise ligand-gated calcium channels. Metabotropic glutamate receptors are G-protein coupled receptors and they activate phopholipases or adenylylcyclase once they are activated.

Glial Fibrillary Acidic Protein (GFAP) Mouse Monoclonal

GluR2/3 Rabbit Polyclonal

Clone: SPM507

Catalog No.

Size

Format

Concentrate

Immunogen: Porcupine spinal cord.

E3000

0.1ml

Concentrate

Concentrate

Application: IHC-P

E3002

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human GluR2/3.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-Use

Species Reactivity: Human*

E3004

7.0ml

E3001

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16510

0.1ml

E16512

0.5ml

E16514 E16511

Isotype: Mouse IgG1

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: Astrocytoma, Brain Cellular Localization: Cytoplasm

Control Tissue: Cerebellum

Human Astrocytoma stained with anti-Glial Fibrillary Acidic Protein (GFAP) antibody E1651

Human Cerebellum stained with anti-GluR2/3 antibody E300

Cellular Localization: Cytoplasm

Product Description: Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system. The antibody to GFAP is useful for documenting astrocytic differentiation in tumors outside the CNS.

Product Description: Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1-7, NMDA1-3, KAI-2), which comprise ligand-gated calcium channels. Metabotropic glutamate receptors are G protein coupled receptors and they activate phopholipases or adenylylcyclase once they are activated.

Glucagon Rabbit Polyclonal

GluR6/7 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2930

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human glucagon.

E2932

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human GluR6/7.

1.0ml

Concentrate

Application: IHC-P

E2934

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2931

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18180

0.1ml

Concentrate

E18182

0.5ml

E18184 E18181

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas

Control Tissue: Brain

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Pancreas stained with anti-Glucagon antibody E1818

Human Brain stained with anti-GluR6/7 antibody E293

Product Description: Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family.

Product Description: Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1-7, NMDA1-3, KAI-2), which comprise ligand-gated calcium channels. Metabotropic glutamate receptors are G-protein coupled receptors and they activate phopholipases or adenylylcyclase once they are activated.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

55


GLUT-1 Rabbit Polyclonal

Glutamic Acid Decarboxylase 65 (GAD65) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3310

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human GLUT-1 protein.

E3312

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human GAD65.

1.0ml

Concentrate

Application: IHC-P

E3314

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3311

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2840

0.1ml

Concentrate

E2842

0.5ml

E2844 E2841

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Esophagus

Control Tissue: Pancreas

Cellular Localization: Membrane

Cellular Localization: Cytoplasm

Human Esophagus stained with anti-GLUT-1 antibody E284

Human Pancreas stained with anti-Glutamic Acid Decarboxylase 65 (GAD65) antibody E331

Product Description: Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast.

Product Description: Glutamate decarboxylase 2 (GAD65) catalyzes the production of Îł-Aminobutyric acid (GABA). Adults express two forms of glutamate decarboxylase (GAD), GAD65 and GAD67. Both are expressed in most GABA containing neurons. GAD65 appears to be targeted to the membrane and nerve endings; GAD65 is predominantly expressed in pancreatic beta cells. Autoantibodies to GAD65 are strongly associated with insulin-dependent diabetes mellitus.

GLUT-1 Mouse Monoclonal

Glutamic Acid Decarboxylase 67 (GAD67) Rabbit Polyclonal Clone: SPM498

Catalog No.

Size

Format

E10260

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human GLUT-1 protein.

E10262

0.5ml

Concentrate

Immunogen: Synthetic peptide from near N-terminus of mouse GAD67 protein.

1.0ml

Concentrate

Application: IHC-P

E10264

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E10261

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E13810

0.1ml

Concentrate

E13812

0.5ml

E13814 E13811

Clone: N/A

Species Reactivity: Human*

Isotype: Mouse IgG2a/k

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Esophagus

Control Tissue: Brain, Pancreas

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane

Human Esophagus stained with anti-GLUT-1 antibody E1381

Human Pancreas stained with anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody E1026

Product Description: Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast.

Product Description: Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to gamma-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. GAD67 is a cytoplasmic protein and is encoded on chromosome 2.

GLUT-3 Rabbit Polyclonal

Glypican-3 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3860

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human GLUT-3 protein.

M3862

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

M3864

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3861

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3270

0.1ml

Concentrate

E3272

0.5ml

E3274 E3271

Isotype: Rabbit IgG Control Tissue: Placenta Cellular Localization: Cytoplasm

Clone: SP86 Immunogen: Synthetic peptide derived from C-terminus of human glypican-3 protein. Application: IHC-P, WB Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Placenta Cellular Localization: Membrane Control Lysate: HepG2 Molecular Weight: 64 kDa

Human Placenta stained with anti-GLUT-3 antibody E327

Human Placenta stained with anti-Glypican-3 antibody M386

Product Description: Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (GLUT) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT-3 staining is also detectable in human cancers including those of the ovary, lung, and testis.

Product Description: Expression alteration of glypican-3 (GPC3) is associated with several malignancies and has been identified as an overexpressed gene in hepatocellular carcinoma (HCC). Glypican-3 is a cell surface proteoglycan that bears heparan sulfate (HS). It may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs.

56

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Glypican-3 Rabbit Polyclonal

Granzyme B Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2580

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human glypican-3 protein.

E2582

0.5ml

Concentrate

Immunogen: Synthetic peptide from N-terminus of human granzyme B.

1.0ml

Concentrate

Application: IHC-P

E2584

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2581

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18870

0.1ml

Concentrate

E18872

0.5ml

E18874 E18871

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Liver Carcinoma

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Human Liver Carcinoma stained with anti-Glypican-3 antibody E1887

Human Tonsil stained with anti-Granzyme B antibody E258

Product Description: Expression alteration of glypican-3 (GPC3) is associated with several malignancies and has been identified as an overexpressed gene in hepatocellular carcinoma (HCC). Glypican-3 is a cell surface proteoglycan that bears heparan sulfate (HS). It may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs.

Product Description: Granzyme A and granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and are stored within cytolytic granules and released by effector cells during degradation. This antibody should be useful for the localization of granzyme B-containing lytic granules and for the characterization of activated CTLs or NK cells.

GNB2 Rabbit Polyclonal

GRB2-Associated Binder 1 (GAB-1) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3620

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from near N-terminus of human GNB2 protein.

E3622

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human GAB-1.

1.0ml

Concentrate

Application: IHC-P

E3624

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3621

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17940

0.1ml

Concentrate

E17942

0.5ml

E17944 E17941

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Astrocytoma

Cellular Localization: Membrane

Cellular Localization: Cytoplasm

Human Placenta stained with anti-GNB2 antibody E1794

Human Astrocytoma stained with anti-GRB2-Associated Binder 1 (GAB-1) antibody E362

Product Description: Guanine nucleotide-binding protein subunit beta-2 (GNB2) is one of the three subunits (alpha, beta and gamma) for guanine nucleotide binding proteins (G proteins). G proteins are membrane associated and heterotrimeric proteins. G proteins and their receptors (GPCRs) form one of the most prevalent signaling systems in mammalian cells. G protein signaling system regulates sensory perception, cell growth, and hormonal regulation.

Product Description: GAB-1 is a multisubstrate docking protein downstream in the signaling pathways of different receptor tyrosine kinases, including the epidermal growth factor receptor (EGFR) and appears to play a crucial role in cellular growth response, transformation, and apoptosis. Experimental data indicates that GAB1 coupling to the Met receptor requires binding of GRB2 and correlates with the transforming potential of Tpr-Met.

GP200 (Renal Cell Carcinoma Marker) Mouse Monoclonal

GRP75 Rabbit Polyclonal

Clone: SPM487

Catalog No.

Size

Format

Concentrate

Immunogen: Recombinant human gp200 protein.

E12490

0.1ml

Concentrate

Concentrate

Application: IHC-P

E12492

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 661-679 of human GRP75 protein.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-Use

Species Reactivity: Human*

E12494

7.0ml

E12491

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E11050

0.1ml

E11052

0.5ml

E11054 E11051

Isotype: Mouse IgG1/k

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: Kidney, Renal Cell Carcinoma Cellular Localization: Cytoplasm, Membrane

Control Tissue: Colon Carcinoma

Human Renal Cell Carcinoma stained with anti-GP200 (Renal Cell Carcinoma Marker) antibody E1105

Human Colon Carcinoma stained with anti-GRP75 antibody E1249

Product Description: GP200 is localized along the brush border of renal tubule and Bowman’s capsule, the luminal surfaces of breast lobules and ducts and the epididymal tubular epithelium, cytoplasm of parathyroid parenchymal cells and the colloid of thyroid follicles. GP200 is reportedly expressed by 93% of primary and 84% of metastatic renal cell carcinomas.

Cellular Localization: Cytoplasm

Product Description: The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

57


GRP78 Rabbit Polyclonal

GSK-3 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2910

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to near N-terminus of human GRP78 protein.

E2912

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human GSK-3.

1.0ml

Concentrate

Application: IHC-P

E2914

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2911

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E12570

0.1ml

Concentrate

E12572

0.5ml

E12574 E12571

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Brain

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-GRP78 antibody E1257

Human Brain stained with anti-GSK-3 antibody E291

Product Description: The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. GRP78 is localized in the endoplasmic reticulum where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains.

Product Description: Glycogen Synthase Kinase-3 (GSK-3) differs from most serine/ threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK-3α and GSK-3β. The function of GSK-3 is to phosphorylate glycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3.

GRP94 Rabbit Polyclonal

Guanylyl Cyclase beta Rabbit Polyclonal

Catalog No.

Size

Format

E13860

0.1ml

Concentrate

E13862

0.5ml

Concentrate

E13864

1.0ml

Concentrate

E13861

7.0ml

Ready-to-Use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to C-terminus of human GRP94 protein.

E10270

0.1ml

Concentrate

E10272

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human guanylyl cyclase beta 1, soluble.

E10274

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

E10271

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm Control Lysate: HeLa Molecular Weight: 94 kDa

Human Brain stained with anti-Guanylyl Cyclase beta antibody E1027

Human Breast Carcinoma stained with anti-GRP94 antibody E1386

Product Description: Glucose-regulated protein 94kDa (GRP-94), also known as endoplasmin, is a member of human heat shock protein (HSP) 90 family. GRP-94 is a cytosolic protein that participates in signaling pathways with PKC ε to protect cells from external damage, particularly in heat shock-mediated events. GRP-94 localizes to the ER and is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.

Product Description: Soluble guanylyl cyclase catalyses in a Mg2+ or Mn2+ dependent manner, the conversion of guanosine-5’-triphosphate (GTP) to cyclic guanosine-3’,5monophosphate (cGMP) and pyrophosphate. This enzyme is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule.

GRSF-1 Rabbit Polyclonal

H. Pylori Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

0.1ml

Concentrate

Immunogen: Purified Helicobacter pylori.

Concentrate

Immunogen: Synthetic peptide derived from internal region of human GRSF-1 protein.

E3010 E3012

0.5ml

Concentrate

Application: IHC-P

1.0ml

Concentrate

Application: IHC-P

E3014

1.0ml

Concentrate

Ready-to-Use

E3011

7.0ml

Ready-to-Use

Species Reactivity: Human*

7.0ml

Catalog No.

Size

Format

E18690

0.1ml

Concentrate

E18692

0.5ml

E18694 E18691

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: H. Pylori Infected Tissue

Control Tissue: Brain

Cellular Localization: H. Pyloric

Cellular Localization: Cytoplasm

Human Brain stained with anti-GRSF-1 antibody E1869

Product Description: G-rich sequence factor 1 (GRSF-1) is a cytoplasmic protein that binds RNAs containing the G-rich element. GRSF-1 is up-regulated with prostate cancer progression and down regulated by the differentiating agents phenylacetate and phenylbutyrate. The function of GRSF-1 is uncharacterized, but potential roles include mRNA transport, stabilization, and translation. It might be involved in prostate cancer metastasis.

58

Human H. Pylori infected Stomach stained with anti-H. Pylori antibody E301

Product Description: Helicobacter pylori (H. Pylori) is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low grade lymphoma of mucosa-associated lymphoid tissue in the stomach.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies H. Pylori Mouse Monoclonal

Heat Shock Protein 60 (HSP60) Rabbit Polyclonal Clone: SPM526

Catalog No.

Size

Format

Concentrate

Immunogen: Purified helicobacter pylori.

E12450

0.1ml

Concentrate

Concentrate

Application: IHC-P

E12452

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 31-50 of human HSP60 protein.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P, WB

Ready-to-Use

Species Reactivity: Human*

E12454

7.0ml

E12451

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18380

0.1ml

E18382

0.5ml

E18384 E18381

Isotype: Mouse IgG1/k

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: H. Pylori Infected Tissue

Control Tissue: Colon Carcinoma

Cellular Localization: H. Pyloric

Cellular Localization: Cytoplasm Control Lysate: HeLa Molecular Weight: 60 kDa

Human H. Pylori infected Stomach stained with anti-H. Pylori antibody E1838

Human Colon Carcinoma stained with anti-Heat Shock Protein 60 (HSP60) antibody E1245

Product Description: Helicobacter pylori (H. Pylori) is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low grade lymphoma of mucosa-associated lymphoid tissue in the stomach.

Product Description: A wide variety of environmental and pathophysiological stressful condition triggers the synthesis of heat shock proteins or stress response proteins. HSP60 is a potential antigen in a number of autoimmune diseases including human arthritis.

HDAC1 Rabbit Polyclonal

Heat Shock Protein 70 (HSP70) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E12330

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human HDAC1 protein.

E12332

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E12334

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E12331

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3550

0.1ml

Concentrate

E3552

0.5ml

E3554 E3551

Clone: N/A Immunogen: Synthetic peptide corresponding to aa 574-591 of human HSP70 protein. Application: IHC-P, WB Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Tonsil

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Nucleus Control Lysate: 293T Molecular Weight: 70 kDa

Human Breast Carcinoma stained with anti-HDAC1 antibody E355

Human Breast Carcinoma stained with anti-Heat Shock Protein 70 (HSP70) antibody E1233

Product Description: Mammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing.

Product Description: The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, they participate in the translocation of polypeptides across cell membranes and to the nucleus and they aid in the proper folding of nascent polypeptide chain. HSP70 expression is strongly induced in response to heat stress.

HDAC2 Rabbit Polyclonal

hENT1** Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M4200

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human HDAC2.

M4202

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human hENT1 protein.

1.0ml

Concentrate

Application: IHC-P, WB

M4204

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M4201

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E10640

0.1ml

Concentrate

E10642

0.5ml

E10644 E10641

Clone: SP120

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Pancreas, Tonsil

Cellular Localization: Nucleus

Cellular Localization: Membrane

Control Lysate: K562 Molecular Weight: 60 kDa Human Tonsil stained with anti-HDAC2 antibody E1064

Product Description: Histone acetylation is a dynamic process whose levels are determined by the net activities of histone acetyltransferases (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

Human Pancreas stained with anti-hENT1 antibody M420

Product Description: Human equilibrative nucleoside transporter 1 (hENT1) is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein in the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides. Nucleoside transporters such as hENT1 are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways. These transporters are necessary for the uptake of cytotoxic nucleosides used for chemotherapies.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

59


HMGA2 Rabbit Polyclonal

ID3 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E14050

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human HMGA2.

E14052

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human ID3 protein.

1.0ml

Concentrate

Application: IHC-P

E14054

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E14051

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16330

0.1ml

Concentrate

E16332

0.5ml

E16334 E16331

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Breast Carcinoma stained with anti-HMGA2 antibody E1633

Human Breast Carcinoma stained with anti-ID3 antibody E1405

Product Description: HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 share 95% amino acid sequence identity.

Product Description: DNA-binding protein inhibitor ID-3 (ID3) is a basic helix-loophelix (bHLH) protein that inhibits muscle-specific gene expression by the binding of E2A-containing protein complexes to the muscle creatine kinase E-box enhancer. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation.

Human Growth Hormone (HGH) Rabbit Polyclonal

IKAROS Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Purified human growth hormone.

M4080

0.1ml

Concentrate

Concentrate

Application: IHC-P

M4082

0.5ml

Concentrate

Immunogen: Synthetic peptide near the N-terminus of human IKAROS.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-Use

Species Reactivity: Human*

M4084

7.0ml

M4081

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17470

0.1ml

E17472

0.5ml

E17474 E17471

Isotype: Rabbit IgG

Clone: SP108

Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: Anterior Pituitary Cellular Localization: Cytoplasm

Control Tissue: Tonsil

Human Anterior Pituitary stained with anti-Human Growth Hormone (HGH) antibody E1747

Human Tonsil stained with anti-IKAROS antibody M408

Cellular Localization: Nucleus

Product Description: HGH is produced by the somatotroph cells in the pituitary. HGH acts through binding to membrane receptors. Ligand binding induces receptor dimerization and activation of the receptor-associated kinase. This results in phosphorylation of the kinase itself, of the receptor, and of many cellular proteins including the Stat proteins as well as adaptors leading to the activation of the Ras/MAP kinase pathway and of the PI-3 kinase pathway.

Product Description: DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B cell and T cell development.

ID2 Rabbit Polyclonal

IKAROS Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E19000

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human ID2 protein.

E19002

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human IKAROS protein.

1.0ml

Concentrate

Application: IHC-P

E19004

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E19001

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16410

0.1ml

Concentrate

E16412

0.5ml

E16414 E16411

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Tonsil

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Breast Carcinoma stained with anti-ID2 antibody E1641

Human Tonsil stained with anti-IKAROS antibody E1900

Product Description: Inhibitor of differentiation 2 (ID2) protein is a transcriptional modulator. ID2 participates in the differentiation and cell cycle progression in organisms. It belongs to a protein family characterized by a helix-loop-helix (HLH) region, but lacking the basic amino acid domain required to bind to DNA. Id2 can disrupt the antiproliferative effects of retinoblastoma (Rb) family tumor suppressor proteins and facilitate the progression of the cell cycle.

60

Clone: N/A

Product Description: DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B cell and T cell development.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Inhibin-alpha Rabbit Polyclonal

IPO-38 (Proliferation Marker) Mouse Monoclonal

Clone: N/A

Catalog No.

Size

Format

E16580

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human inhibin-alpha.

E16582

0.5ml

Concentrate

Immunogen: Spleen cells of a patient with hairy cell leukemia.

1.0ml

Concentrate

Application: IHC-P, WB

E16584

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E16581

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17390

0.1ml

Concentrate

E17392

0.5ml

E17394 E17391

Clone: SPM515

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgM/k

Control Tissue: Testis

Control Tissue: Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Control Lysate: HeLa Molecular Weight: 40 kDa Human Testis stained with anti-Inhibin-alpha antibody E1739

Human Tonsil stained with anti-IPO-38 (Proliferation Marker) antibody E1658

Product Description: Inhibin is a dimeric glycoprotein hormone from the TGF-beta family made up of alpha and beta subunits. It inhibits the production of folliclestimulating hormone from the pituitary while activin stimulates the production of FSH. It is suggested that inhibin may act as a gonadal tumor suppressor.

Product Description: IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin’s disease and non-Hodgkin’s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes.

Integrin beta 5 Rabbit Polyclonal

IRS-1 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E12590

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human integrin beta 5 subunit.

E12592

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 1223-1242 of human IRS-1.

1.0ml

Concentrate

Application: IHC-P

E12594

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E12591

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2860

0.1ml

Concentrate

E2862

0.5ml

E2864 E2861

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Bone Marrow, Colon Carcinoma, Small Intestine

Control Tissue: Colon Carcinoma Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Small Intestine stained with anti-Integrin beta 5 antibody E286

Human Colon Carcinoma stained with anti-IRS-1 antibody E1259

Product Description: Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and vitronectin. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors.

Product Description: The insulin receptor substrate-1 (IRS-1), a 170-185kDa protein major substrate of the insulin receptor is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor (IGF-I) and interleukin 4. IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues.

Involucrin Rabbit Polyclonal

JAB1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E10280

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 420-578 of human involucrin.

E10282

0.5ml

Concentrate

Immunogen: Synthetic peptide from N-terminus of human JAB1 protein.

1.0ml

Concentrate

Application: IHC-P

E10284

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E10281

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E10630

0.1ml

Concentrate

E10632

0.5ml

E10634 E10631

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Skin

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Nucleus

Human Skin stained with anti-Involucrin antibody E1063

Human Breast Carcinoma stained with anti-JAB1 antibody E1028

Product Description: Involucrin is a cytoplasmic protein precursor of the epidermal cornified envelope that becomes cross-linked by transglutaminase during envelope assembly. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea which lacks a distinct cornified layer. The protein is a useful marker of terminal differentiation. It is tightly linked to the onset of differentiation and first expressed in the immediate suprabasal layers of the epidermis.

Product Description: Jun activation domain-binding protein-1 (JAB1) also designated COP9 subunits 5 (COPS5) or SGN5 is a coactivator of AP-1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP-1 containing complexes, and enhances transactivation from AP-1dependent promoters. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both in the nucleus and the cytoplasm.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

61


J-chain Rabbit Monoclonal

Kappa Light Chain (B Cell Marker) Mouse Monoclonal Clone: SP105

Catalog No.

Size

Format

E16520

0.1ml

Concentrate

Immunogen: B-lymphoma cells.

Concentrate

Immunogen: Synthetic peptide near the internal region of human J-chain.

E16522

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P, WB

1.0ml

E16524

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*, Mouse*

E16521

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

M4050

0.1ml

Concentrate

M4052

0.5ml

M4054 M4051

Isotype: Rabbit IgG

Clone: SPM508

Isotype: Mouse IgG1 Control Tissue: Tonsil

Control Tissue: Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Control Lysate: Human Spleen Molecular Weight: 25 kDa

Human Tonsil stained with anti-J-chain antibody M405

Human Tonsil stained with anti-Kappa Light Chain (B Cell Marker) antibody E1652

Product Description: J chain is a small glycopeptide and is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells. J chain is linked to IgA and IgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells. B cells secrete J chain at an early stage of differentiation. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsil and lymph node.

Product Description: Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. Kappa light chain contains one immunoglobulin like domain.

J-chain Rabbit Polyclonal

Keratin 5 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3270

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human J-chain protein.

M3272

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human cytokeratin 5.

1.0ml

Concentrate

Application: IHC-P

M3274

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3271

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E19010

0.1ml

Concentrate

E19012

0.5ml

E19014 E19011

Clone: SP27

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Breast, Mesothelioma, Prostate, Skin

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm Control Lysate: A431 Molecular Weight: 58 kDa

Human Tonsil stained with anti-J-chain antibody E1901

Human Prostate Adenocarcinoma stained with anti-Keratin 5 antibody M327

Product Description: J chain is a small glycopeptide and is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells. J chain is linked to IgA and IgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells. B cells secrete J chain at an early stage of differentiation. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsil and lymph node.

Product Description: Twenty human keratins are divided into acidic and basic subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Human keratins are useful markers in cancer research. Cytokeratin 5 is expressed in breast myoepithelia cells, prostate basal cells, most epithelial and biphasic mesotheliomas.

Jena-Muenchen4 (JM4) Rabbit Polyclonal

Keratin 5 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E13820

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to aa 165-178 of human JM4.

E13822

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 5.

1.0ml

Concentrate

Application: IHC-P

E13824

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E13821

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E14000

0.1ml

Concentrate

E14002

0.5ml

E14004 E14001

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Breast, Mesothelioma, Prostate, Skin

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Human Brain stained with anti-Jena-Muenchen4 (JM4) antibody E1400

Human Skin stained with anti-Keratin 5 antibody E1382

Product Description: JM4 is a 19kDa four-transmembrane protein binding to the CCR5 receptor. It plays a role in the regulation of intracellular protein transport. It is expressed in normal brain, small intestine, lung, spleen, and pancreas and has also been detected in tumors of breast, colon, lung, and ovary.

62

Clone: N/A

Product Description: Twenty human keratins are divided into acidic and basic subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Human keratins are useful markers in cancer research. Cytokeratin 5 is expressed in breast myoepithelia cells, prostate basal cells, most epithelial and biphasic mesotheliomas.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Keratin 6 Rabbit Monoclonal

Keratin 7 Rabbit Polyclonal Clone: SP87

Catalog No.

Size

Format

E18000

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human keratin 6 protein.

E18002

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 7.

1.0ml

Concentrate

Application: IHC-P, WB

E18004

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18001

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3870

0.1ml

Concentrate

M3872

0.5ml

M3874 M3871

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate, Skin

Control Tissue: Breast Carcinoma, Ovarian Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Control Lysate: A431 Molecular Weight: 60 kDa

Control Lysate: HeLa

Human Prostate Carcinoma stained with anti-Keratin 6 antibody M387

Human Breast Carcinoma stained with anti-Keratin 7 antibody E1800

Molecular Weight: 54 kDa

Product Description: Cytokeratin 6 (CK6) and 16 (CK16) are expressed in keratinocytes, which undergo rapid turnover in the suprabasal region. CK6 is found in basal cells of prostate glands, hair follicles, suprabasal cells of a variety of internal stratified epithelia and in epidermis. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas.

Product Description: Cytokeratin 7 (CK7) is a basic cytokeratin which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract.

Keratin 6 Rabbit Polyclonal

Keratin 8 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M4020

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human cytokeratin 6 protein.

M4022

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human keratin 8.

1.0ml

Concentrate

Application: IHC-P

M4024

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M4021

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18880

0.1ml

Concentrate

E18882

0.5ml

E18884 E18881

Clone: SP102

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate, Skin

Control Tissue: Breast Ductal Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Prostate stained with anti-Keratin 6 antibody E1888

Human Breast Ductal Carcinoma stained with anti-Keratin 8 antibody M402

Product Description: Cytokeratin 6 (CK6) and 16 (CK16) are expressed in keratinocytes, which undergo rapid turnover in the suprabasal region. CK6 is found in basal cells of prostate glands, hair follicles, suprabasal cells of a variety of internal stratified epithelia and in epidermis. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas.

Product Description: Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in association with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia. It is present in hepatocellular carcinomas and the majority of adenocarcinomas and ductal carcinomas, but it is absent in squamous cell carcinomas.

Keratin 7** Rabbit Monoclonal

Keratin 8 Rabbit Polyclonal

Clone: SP52

Catalog No.

Size

Format

E3260

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 7.

E3262

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 1-483 of human cytokeratin 8.

1.0ml

Concentrate

Application: IHC-P, WB

E3264

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3261

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3520

0.1ml

Concentrate

M3522

0.5ml

M3524 M3521

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Ovarian Carcinoma

Control Tissue: Breast Carcinoma, Lung, Skin

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Control Lysate: HeLa

Control Lysate: HeLa

Molecular Weight: 54 kDa

Molecular Weight: 52 kDa

Human Breast Carcinoma stained with anti-Keratin 7 antibody M352

Human Lung stained with anti-Keratin 8 antibody E326

Product Description: Cytokeratin 7 (CK7) is a basic cytokeratin which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not in colon, prostate, or gastrointestinal tract.

Product Description: Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in association with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia. It is present in hepatocellular carcinomas and the majority of adenocarcinomas and ductal carcinomas, but it is absent in squamous cell carcinomas.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

63


Keratin 8 Rabbit Polyclonal

Keratin 14 Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3530

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from the human cytokeratin 8.

M3532

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 14.

1.0ml

Concentrate

Application: IHC-P

M3534

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3531

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18400

0.1ml

Concentrate

E18402

0.5ml

E18404 E18401

Clone: SP53

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Colon Carcinoma

Control Tissue: Prostate, Skin, Squamous Cell Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm Control Lysate: A431 Molecular Weight: 50 kDa

Human Colon Carcinoma stained with anti-Keratin 8 antibody E1840

Human Prostate stained with anti-Keratin 14 antibody M353

Product Description: Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in association with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia. It is present in hepatocellular carcinomas and the majority of adenocarcinomas and ductal carcinomas, but it is absent in squamous cell carcinomas.

Product Description: Cytokeratin 14 (CK14) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 5.

Keratin 10** Rabbit Monoclonal

Keratin 14 Rabbit Polyclonal

Clone: SP99

Catalog No.

Size

Format

E2620

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human keratin 10.

E2622

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human cytokeratin 14.

1.0ml

Concentrate

Application: IHC-P

E2624

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2621

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3990

0.1ml

Concentrate

M3992

0.5ml

M3994 M3991

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Skin

Control Tissue: Skin, Squamous Cell Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Skin stained with anti-Keratin 10 antibody M399

Human Skin stained with anti-Keratin 14 antibody E262

Product Description: Cytokeratin 10 is a type I cytokeratin, expressed by suprabasal cells of stratified and cornified epithelia. Its defects are implicated in bullous congenital ichthyosiform erythroderma, epidermolytic hyperkeratosis and diffuse nonepidermolytic palomoplantar keratoderma.

Product Description: Cytokeratin 14 (CK14) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 5.

Keratin 10 Rabbit Polyclonal

Keratin 17** Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3950

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human keratin 10 protein.

M3952

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

M3954

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3951

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18980

0.1ml

Concentrate

E18982

0.5ml

E18984 E18981

Isotype: Rabbit IgG Control Tissue: Skin Cellular Localization: Cytoplasm

Clone: SP95 Immunogen: Synthetic peptide corresponding to C-terminus of human keratin 17 protein. Application: IHC-P, WB Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Prostate Carcinoma Cellular Localization: Cytoplasm Control Lysate: HeLa Molecular Weight: 48 kDa

Human Skin stained with anti-Keratin 10 antibody E1898

Product Description: Cytokeratin 10 is a type I cytokeratin, expressed by suprabasal cells of stratified and cornified epithelia. Its defects are implicated in bullous congenital ichthyosiform erythroderma, epidermolytic hyperkeratosis and diffuse nonepidermolytic palomoplantar keratoderma.

64

Human Prostate Carcinoma stained with anti-Keratin 17 antibody M395

Product Description: Cytokeratin 17 (CK17) plays a role in the formation and maintenance of various skin appendages cytokeratin 17 is expressed in the outer root sheath and medulla region of hair follicle from mucosal stratified squamous epithelia, basal cells of oral epithelium, palmoplantar epidermis, and sweat and mammary glands. It is also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Keratin 17 Rabbit Polyclonal

Keratin 18 Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E17010

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of cytokeratin 17 protein.

E17012

0.5ml

Concentrate

Immunogen: Human breast cancer PMC42 cells.

1.0ml

Concentrate

Application: IHC-P, WB

E17014

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17011

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18920

0.1ml

Concentrate

E18922

0.5ml

E18924 E18921

Clone: DC10

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgG1

Control Tissue: Lung Adenocarcinoma, Prostate Carcinoma

Control Tissue: Breast Carcinoma, Skin

Cellular Localization: Cytoplasm

Control Lysate: A431

Cellular Localization: Cytoplasm

Control Lysate: HeLa

Molecular Weight: 48 kDa

Molecular Weight: 48 kDa Human Lung Adenocarcinoma stained with anti-Keratin 17 antibody E1892

Human Breast Carcinoma stained with anti-Keratin 18 antibody E1701

Product Description: Cytokeratin 17 (CK17) plays a role in the formation and maintenance of various skin appendages cytokeratin 17 is expressed in the outer root sheath and medulla region of hair follicle from mucosal stratified squamous epithelia, basal cells of oral epithelium, palmoplantar epidermis, and sweat and mammary glands. It is also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix.

Product Description: Cytokeratin 18 (CK18), which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14.

Keratin 18 Rabbit Monoclonal

Keratin 18 Mouse Monoclonal Clone: SP69

Catalog No.

Size

Format

E16540

0.1ml

Concentrate

Immunogen: Human breast cancer PMC42 cells

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 18.

E16542

0.5ml

Concentrate

Concentrate

Application: IHC-P, WB

Application: IHC-P

1.0ml

E16544

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E16541

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

M3690

0.1ml

Concentrate

M3692

0.5ml

M3694 M3691

Isotype: Rabbit IgG

Clone: SPM510

Isotype: Mouse IgG1 Control Tissue: Breast Carcinoma, Skin

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm Control Lysate: A431 Molecular Weight: 48 kDa Human Breast Carcinoma stained with anti-Keratin 18 antibody M369

Human Skin stained with anti-Keratin 18 antibody E1654

Product Description: Cytokeratin 18 (CK18), which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14.

Product Description: Cytokeratin 18 (CK18), which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14.

Keratin 18 Rabbit Polyclonal

Keratin 19 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2630

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 18

E2632

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human cytokeratin 19.

1.0ml

Concentrate

Application: IHC-P, WB

E2634

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2631

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18330

0.1ml

Concentrate

E18332

0.5ml

E18334 E18331

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Skin

Control Tissue: Skin

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Control Lysate: A431 Molecular Weight: 48 kDa Human Breast Carcinoma stained with anti-Keratin 18 antibody E1833

Product Description: Cytokeratin 18 (CK18), which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14.

Human Skin stained with anti-Keratin 19 antibody E263

Product Description: Cytokeratin 19 (CK19) is a member of type I acidic subfamily of intermediate filaments. It is expressed in various different human tissues except in liver. Cytokeratin 19 is not expressed in hepatocytes.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

65


Keratin 20** Rabbit Monoclonal

Ki-67 Rabbit Polyclonal

Clone: SP33

Catalog No.

Size

Format

E1870

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human cytokeratin 20.

E1872

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from the human Ki-67 protein.

1.0ml

Concentrate

Application: IHC-P

E1874

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E1871

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3330

0.1ml

Concentrate

M3332

0.5ml

M3334 M3331

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Breast Carcinoma, Lymph Node, Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Colon Carcinoma stained with anti-Keratin 20 antibody M333

Human Tonsil stained with anti-Ki-67 antibody E187

Product Description: Cytokeratin 20 (CK20) is a Type-I keratin primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system, mucinous ovarian tumors, transitional-cell, and Merkel-cell carcinomas. CK20 is negative in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas.

Product Description: Ki-67 is a nuclear protein which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein.

Keratin 20 Rabbit Polyclonal

KOC/L523S Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18310

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 20.

E18312

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human KOC/L523S.

1.0ml

Concentrate

Application: IHC-P

E18314

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18311

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16440

0.1ml

Concentrate

E16442

0.5ml

E16444 E16441

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Placenta, Prostate Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Colon Carcinoma stained with anti-Keratin 20 antibody E1644

Human Prostate Carcinoma stained with anti-KOC/L523S antibody E1831

Product Description: Cytokeratin 20 (CK20) is a Type-I keratin primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system, mucinous ovarian tumors, transitional-cell, and Merkel-cell carcinomas. CK20 is negative in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas.

Product Description: Insulin-like growth factor-II messenger RNA (mRNA)-binding protein-3 (IMP-3), also known as K homology domain-containing protein (KOC), is a member of the insulin-like growth factor-II mRNA-binding protein family and is expressed in some malignancies such as melanoma but not in benign nevi. IMP-3 is expressed in a significantly higher proportion of melanomas than Spitz nevi. IMP-3 appears to be involved in the progression of malignant melanoma.

Ki-67 Rabbit Monoclonal

k-Ras Rabbit Polyclonal Clone: SP6

Catalog No.

Size

Format

E17960

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human Ki-67 protein.

E17962

0.5ml

Concentrate

Immunogen: Synthetic peptide from internal region of human k-Ras protein.

1.0ml

Concentrate

Application: IHC-P

E17964

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*, Mouse*

E17961

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3060

0.1ml

Concentrate

M3062

0.5ml

M3064 M3061

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Bladder, Breast, Prostate Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Membrane

Human Tonsil stained with anti-Ki-67 antibody M306

Human Prostate Carcinoma stained with anti-k-Ras antibodyy E1796

Product Description: Ki-67 is a nuclear protein which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein.

66

Clone: N/A

Product Description: The guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D).

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Ku80 Rabbit Polyclonal

Lamin B Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E13490

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human Ku80 protein.

E13492

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human lamin B.

1.0ml

Concentrate

Application: IHC-P

E13494

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E13491

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E19120

0.1ml

Concentrate

E19122

0.5ml

E19124 E19121

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus Envelope Control Lysate: HepG2 Molecular Weight: 67 kDa

Human Placenta stained with anti-Ku80 antibody E1912

Human Breast Carcinoma stained with anti-Lamin B antibody E1349

Product Description: X-ray repair cross-complementing protein 5 (XRCC5), alternative name (Ku80), is a single stranded DNA-dependent ATP-dependent helicase. Ku80, along with Ku70 forms a heterodimeric protein and Ku80 is a widely expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks. The inhibition of Ku80 can damage the DNA repair pathway of tumor cells and potentially increase the killing of cancer cells.

Product Description: Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing: lamin A, lamin C and lamin Adel10. While the B-type lamins include two proteins arising from two distinct genes: lamin B1 and lamin B2.

Ku80 Rabbit Polyclonal

Lewis A Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E17380

0.1ml

Concentrate

Immunogen: Mucin isolated from an ovarian cyst.

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human Ku80 protein.

E17382

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P

1.0ml

E17384

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17381

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E19110

0.1ml

Concentrate

E19112

0.5ml

E19114 E19111

Isotype: Rabbit IgG

Clone: SPM522

Isotype: Mouse IgG1 Control Tissue: Colon Carcinoma

Control Tissue: Placenta

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Placenta stained with anti-Ku80 antibody E1911

Human Colon Carcinoma stained with anti-Lewis A antibody E1738

Product Description: X-ray repair cross-complementing protein 5 (XRCC5), alternative name (Ku80), is a single stranded DNA-dependent ATP-dependent helicase. Ku80, along with Ku70 forms a heterodimeric protein and Ku80 is a widely expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks. The inhibition of Ku80 can damage the DNA repair pathway of tumor cells and potentially increase the killing of cancer cells.

Product Description: Lewis A is a blood group antigen consisting of Gal Beta1-3 (Fuc alpha1-4) GlcNAc. Lewis A is found to be present primarily on epithelial cells such as colon and kidneys.

Lambda Light Chain Rabbit Polyclonal

LIMD1 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E18700

0.1ml

Concentrate

Concentrate

Immunogen: Free lambda light chain from pooled human IgG lambda myeloma sera.

E18702

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human LIMD1 protein.

1.0ml

Concentrate

Application: IHC-P, WB

E18704

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18701

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E19200

0.1ml

Concentrate

E19202

0.5ml

E19204 E19201

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Lung, Lung Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Nucleus

Control Lysate: Human Spleen Molecular Weight: 25 kDa Human Tonsil stained with anti-Lambda Light Chain antibody E1920

Product Description: Lambda light chains are polypeptide chains located in the cell membrane and cytoplasmic regions of normal B cells and plasma cells. The combination of lambda light chains and heavy chains forms immunoglobulin molecules. There are two classes of light chains found in immunoglobulins: kappa light chains and lambda light chains. Light chain production by lymphoid cells is genetically restricted to single light chain class.

Human Lung Carcinoma stained with anti-LIMD1 antibody E1870

Product Description: The Zyxin family of proteins contains five members: Ajuba, LIMD1, LPP, TRIP6 and Zyxin. LIMD1 is a ubiquitously expressed tumor suppressor. LIM domains consist of a cysteine-rich consensus sequence containing two distinct zinc-binding subdomains, which mediate protein-protein interactions. LIMD1 was first identified when the deletion of its gene was noted in some cervical cancers. LIMD1 blocks tumor growth and is downregulated in lung cancer.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

67


Lipocalin-2 Rabbit Polyclonal

LMO2 Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E17650

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human lipocalin-2 protein.

E17652

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to human LMO2 protein.

1.0ml

Concentrate

Application: IHC-P

E17654

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17651

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18810

0.1ml

Concentrate

E18812

0.5ml

E18814 E18811

Clone: 1A9-3D11

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgG1

Control Tissue: Breast Carcinoma, Stomach Carcinoma

Control Tissue: Hodgkin’s Lymphoma, Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Stomach Carcinoma stained with anti-Lipocalin-2 antibody E1881

Human Tonsil stained with anti-LMO2 antibody E1765

Product Description: Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D and odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances.

Product Description: LMO-2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO-2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO-2 transcription start site is located approximately 25 kb downstream from the 11p13 T cell translocation cluster (11p13 ttc), where T cell acute lymphoblastic leukemia-specific translocations occur.

LMO2** Rabbit Monoclonal

Luteinizing Hormone (LH) Rabbit Polyclonal

Catalog No.

Size

Format

M3510

0.1ml

Concentrate

M3512

0.5ml

Concentrate

M3514

1.0ml

Concentrate

M3511

7.0ml

Ready-to-Use

Clone: SP51

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to near N-terminus of human LMO2 protein.

E17480

0.1ml

Concentrate

Immunogen: Purified human luteinizing hormone.

E17482

0.5ml

Concentrate

Application: IHC-P

E17484

1.0ml

Concentrate

Application: IHC-P, WB

E17481

7.0ml

Ready-to-Use

Species Reactivity: Human*

Species Reactivity: Human*

Clone: N/A

Isotype: Rabbit IgG Control Tissue: Anterior Pituitary

Isotype: Rabbit IgG

Cellular Localization: Cytoplasm

Control Tissue: Hodgkin’s Lymphoma, Tonsil Cellular Localization: Nucleus Control Lysate: Ramos Molecular Weight: 24 kDa Human Tonsil stained with anti-LMO2 antibody M351

Human Anterior Pituitary stained with anti-Luteinizing Hormone (LH) antibody E1748

Product Description: LMO-2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO-2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO-2 transcription start site is located approximately 25 kb downstream from the 11p13 T cell translocation cluster (11p13 ttc), where T cell acute lymphoblastic leukemia-specific translocations occur.

Product Description: LH is a tropic hormone which modulates the secretory activity of other endocrine glands. It is produced in the anterior hypophysis of the pituitary gland. The glycoprotein hormone, LH, like follicle stimulating hormone and thyroid stimulating hormone, is composed of a common alpha-subunit but also a specific beta-subunit, which characterizes each of these hormones.

LMO2 Rabbit Polyclonal

MAGE-1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E4500

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to human LMO2 protein.

E4502

0.5ml

Concentrate

Immunogen: Full length recombinant human MAGE-1 protein.

1.0ml

Concentrate

Application: IHC-P

E4504

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4501

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17830

0.1ml

Concentrate

E17832

0.5ml

E17834 E17831

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Hodgkin’s Lymphoma, Tonsil

Control Tissue: Melanoma, Testis

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Tonsil stained with anti-LMO2 antibody E1783

Human Melanoma stained with anti-MAGE-1 antibody E450

Product Description: LMO-2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO-2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO-2 transcription start site is located approximately 25 kb downstream from the 11p13 T cell translocation cluster (11p13 ttc), where T cell acute lymphoblastic leukemia-specific translocations occur.

68

Clone: N/A

Product Description: Melanoma-associated antigen 1 (MAGE-1) genes encode an important group of antigens. MAGE-1 is expressed on a significant proportion of human neoplasms such as melanoma, brain glial tumors, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas, but not on kidney tumor, leukemias and lymphomas.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies MALT-1 Rabbit Polyclonal

MCL-1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2640

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human MALT-1 protein.

E2642

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human MCL-1.

1.0ml

Concentrate

Application: IHC-P, WB

E2644

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2641

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18900

0.1ml

Concentrate

E18902

0.5ml

E18904 E18901

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: B Cell Lymphoma, Lymph Node, Tonsil

Control Tissue: Tonsil Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm, Nucleus Control Lysate: HeLa Molecular Weight: 90 kDa Human B Cell Lymphoma stained with anti-MALT-1 antibody E1890

Human Tonsil stained with anti-MCL-1 antibody E264

Product Description: Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT-1) has two alternatively spliced transcript variants encoding different isoforms. Isoform a is protein of 824 amino acids, and isoform b is a protein of 813 amino acids. MALT-1 is thought to play a role in NF kappaB signaling by enchancing NF kappaB activation through interaction with BCL-10. Interaction between MALT-1 and BCL-10 mediates IKK activation in vitro.

Product Description: Myeloid cell leukemia-1 (MCL-1) is a member of the BCL-2 family. The carboxy terminal of MCL-1 and BCL-2 share significant sequence homology. Expression of MCL-1 is increased upon exposure of ML-1 cells to ionizing radiation, ultraviolet radiation, and alkylating drugs along with increases in GADD45 and Bax and a decrease in BCL-2. Like BCL-2, MCL-1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis.

Mammaglobin Rabbit Polyclonal

MCM2 Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

M3850

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to human mammmaglobin protein.

M3852

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

M3854

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3851

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16400

0.1ml

Concentrate

E16402

0.5ml

E16404 E16401

Clone: SP85 Immunogen: Synthetic peptide derived from internal region of human MCM2 protein. Application: IHC-P, WB Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Rectal Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus Control Lysate: HeLa Molecular Weight: 130 kDa

Human Breast Carcinoma stained with anti-Mammaglobin antibody E1640

Human Rectal Carcinoma stained with anti-MCM2 antibody M385

Product Description: The mammaglobin gene was first identified using a differential screening approach directed at the isolation of novel, human breast cancer-associated genes. Mammaglobin encodes a 10kDa glycoprotein and is distantly related to a family of epithelial secretory proteins. Mammaglobin is overexpressed in human breast cancer. Mammaglobin is a relatively specific mammary marker.

Product Description: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4(CDC21), MCM5(CDC46), MCM6(Mis5) and MCM7(CDC47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. MCM2/MCM4 complexes function as substrates for CDC2/ cyclin B in vitro.

MART-1/Melan-A Rabbit Polyclonal

MCM2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Recombinant human MART-1 protein.

E18730

0.1ml

Concentrate

Concentrate

Application: IHC-P

E18732

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human MCM2 protein.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-Use

Species Reactivity: Human*

E18734

7.0ml

E18731

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2830

0.1ml

E2832

0.5ml

E2834 E2831

Isotype: Rabbit IgG

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: Melanoma Cellular Localization: Cytoplasm

Control Tissue: Rectal Carcinoma

Human Melanoma stained with anti-MART-1/Melan-A antibody E283

Human Rectal Carcinoma stained with anti-MCM2 antibody E1873

Product Description: MART-1 / Melan-A is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-1 tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Cellular Localization: Nucleus

Product Description: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4(CDC21), MCM5(CDC46), MCM6(Mis5) and MCM7(CDC47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. MCM2/MCM4 complexes function as substrates for CDC2/ cyclin B in vitro.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

69


MCM2 Rabbit Polyclonal

MDGI Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18620

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human MCM2 protein.

E18622

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human MDGI protein.

1.0ml

Concentrate

Application: IHC-P

E18624

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18621

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E12480

0.1ml

Concentrate

E12482

0.5ml

E12484 E12481

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Colon Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-MCM2 antibody E1248

Human Colon Carcinoma stained with anti-MDGI antibody E1862

Product Description: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4(CDC21), MCM5(CDC46), MCM6(Mis5) and MCM7(CDC47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. MCM2/MCM4 complexes function as substrates for CDC2/ cyclin B in vitro.

Product Description:MDGI/FABP3 is a small cytosolic protein responsible for the transport and deposition of fatty acids inside the cell.

MCM5 Rabbit Polyclonal

MDM2 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3500

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 1-150 of human MCM5.

E3502

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human MDM2 protein.

1.0ml

Concentrate

Application: IHC-P

E3504

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3501

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E13830

0.1ml

Concentrate

E13832

0.5ml

E13834 E13831

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Prostate stained with anti-MCM5 antibody E1383

Human Breast Carcinoma stained with anti-MDM2 antibody E350

Product Description: MCM5 (Mini Chromosome Maintainance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. G0 phase levels of MCM2 and MCM5 are lower than that of MCM7 and MCM3 but increase as the cell enters G1/S phase of the cell cycle.

Product Description: Human homolog of murine double minute-2 (MDM2) and p53 function in mutual regulation: activation of p53 results in expression of MDM2 which consequently inhibits the ability of wild type p53 to stimulate expression of target genes. MDM2 is frequently overexpressed in the nuclei of soft tissue sarcomas, and to a much lesser extent in a wide variety of malignancies of bladder, breast, testis, cervix, glial cells.

MDGI Rabbit Polyclonal

MEF-2 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E12390

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human MDGI protein.

E12392

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 487-507 of human MEF-2.

1.0ml

Concentrate

Application: IHC-P

E12394

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E12391

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18670

0.1ml

Concentrate

E18672

0.5ml

E18674 E18671

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Brain

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Colon Carcinoma stained with anti-MDGI antibody E1867

Human Brain stained with anti-MEF-2 antibody E1239

Product Description: MDGI/FABP3 is a small cytosolic protein responsible for the transport and deposition of fatty acids inside the cell.

70

Clone: N/A

Product Description:The myocyte enhancer factors-2 (MEF-2) family of transcription factors associate with a variety of co-repressors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four distinct family members arise from alternatively splices transcripts, termed MEF-2A, MEF-2B, MEF-2C, and MEF2D. These members bind as homo- and heterodimers to the MEF2 site in the promoter region of affected genes.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Melanoma (gp100) Rabbit Polyclonal

mGluR1 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E2890

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 1-150 human gp100.

E2892

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human mGluR1.

1.0ml

Concentrate

Application: IHC-P

E2894

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2891

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E4450

0.1ml

Concentrate

E4452

0.5ml

E4454 E4451

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Melanoma

Control Tissue: Brain

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Melanoma stained with anti-Melanoma (gp100) antibody E445

Human Brain stained with anti-mGluR1 antibody E289

Product Description: By immunohistochemistry, this antibody specifically recognizes a protein in melanocytes and melanomas. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Product Description: Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1-7, NMDA1-3, KAI-2) which comprise ligand-gated calcium channels. Metabotropic glutamate receptors are G-protein coupled receptors, which when activated can activate phopholipases or adenylylcyclase.

Mesothelin Rabbit Monoclonal

mGluR5 Rabbit Polyclonal

Clone: SP74

Catalog No.

Size

Format

E2870

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human mesothelin protein.

E2872

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human mGluR5.

1.0ml

Concentrate

Application: IHC-P

E2874

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2871

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3740

0.1ml

Concentrate

M3742

0.5ml

M3744 M3741

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Mesothelioma

Control Tissue: Brain

Cellular Localization: Membrane

Cellular Localization: Cytoplasm

Human Mesothelioma stained with anti-Mesothelin antibody M374

Human Brain stained with anti-mGluR5 antibody E287

Product Description: Mesothelin is a glycocylphosphotidylianositol-linked cell-surface glycoprotein that is present on the surface of normal mesothelium and is overexpressed in epithelial ovarian cancer and malignant mesotheliomas.

Product Description: Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1-7, NMDA1-3, KAI-2) which comprise ligand-gated calcium channels. Metabotropic glutamate receptors are G-protein coupled receptors, which when activated can activate phopholipases or adenylylcyclase.

Mesothelin Rabbit Polyclonal

Microphthalmia (MiTF) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E17900

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from the human mesothelin.

E17902

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to human MiTF.

1.0ml

Concentrate

Application: IHC-P

E17904

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17901

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18390

0.1ml

Concentrate

E18392

0.5ml

E18394 E18391

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Mesothelioma

Control Tissue: Melanoma

Cellular Localization: Membrane

Cellular Localization: Nucleus

Human Mesothelioma stained with anti-Mesothelin antibody E1839

Human Melanoma stained with anti-Microphthalmia (MiTF) antibody E1790

Product Description: Mesothelin is a glycocylphosphotidylianositol-linked cell-surface glycoprotein that is present on the surface of normal mesothelium and is overexpressed in epithelial ovarian cancer and malignant mesotheliomas.

Product Description: Microphthalmia associated transcription factor (MiTF) is a basic helix-loop-helix leucine zipper transcription factor implicated in pigmentation, mast cells and bone development. There are two known isoforms of MiTF. The shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells, as well as mast cells and heart.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

71


MIP-1 alpha Rabbit Polyclonal

MMP-2 (72kDa Collagenase IV) Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E18010

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human MIP-1 alpha.

E18012

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human MMP-2 protein.

1.0ml

Concentrate

Application: IHC-P

E18014

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18011

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E12680

0.1ml

Concentrate

E12682

0.5ml

E12684 E12681

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung Cellular Localization: Cytoplasm, Membrane

Control Tissue: Bladder, Breast, Ovarian Carcinoma, Placenta

Human Lung stained with anti-MIP-1 alpha antibody E1268

Human Placenta stained with anti-MMP-2 (72kDa Collagenase IV) antibody E1801

Cellular Localization: Cytoplasm

Product Description: Macrophage inflammatory protein-1a (MIP-1a) is an 8kDa member of the C-C chemokine subfamily. C-C chemokines are chemoattractants and activators for monocytes and T cells. MIP-1a has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1b and RANTES.

Product Description: MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP-2 is activated by cleavage of the first 80 amino acids. This antibody reacts with both latent and active MMP-2.

MLH1 Rabbit Polyclonal

MMP-3 (Stromelysin-1) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E12670

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human MLH1 protein.

E12672

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 251478 of human MMP-3.

1.0ml

Concentrate

Application: IHC-P

E12674

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E12671

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17810

0.1ml

Concentrate

E17812

0.5ml

E17814 E17811

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Rectal Carcinoma

Control Tissue: Placenta

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Colon Carcinoma stained with anti-MLH1 antibody E1781

Human Placenta stained with anti-MMP-3 (Stromelysin-1) antibody E1267

Product Description: MUTL protein homolog 1 (MLH1) is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintain the integrity of genetic information. An alteration of microsatellite repeats due to strand misalignment during DNA replication is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis.

Product Description: Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) is implicated in the invasive potential of malignant tumors. Stromelysin (MMP3/Transin) degrades several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.

MMP-1 Rabbit Polyclonal

MMP-9 (92kDa Collagenase IV) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3660

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from near C-terminus of human MMP-1 protein.

E3662

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human MMP-9 protein.

1.0ml

Concentrate

Application: IHC-P

E3664

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3661

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3570

0.1ml

Concentrate

E3572

0.5ml

E3574 E3571

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Bladder, Breast, Ovarian Carcinoma, Placenta

Control Tissue: Breast Carcinoma, Placenta

Cellular Localization: Cytoplasm

Human Placenta stained with anti-MMP-1 antibody E357

Product Description: Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) is implicated in the invasive potential of tumors. MMP-1, MMP2, and MMP-9 dissolve extracellular matrix (ECM) and may initiate and promote angiogenesis.

72

Clone: N/A

Cellular Localization: Cytoplasm

Human Placenta stained with anti-MMP-9 (92kDa Collagenase IV) antibody E366

Product Description: MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. This antibody reacts with both latent and active MMP-9.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies MMP-10 (Stromelysin-2) Rabbit Polyclonal

MMP-19 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E4030

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from the hinge domain of human MMP-10 protein.

E4032

0.5ml

Concentrate

Immunogen: Synthetic peptide from N-terminus of human MMP-19 protein.

1.0ml

Concentrate

Application: IHC-P

E4034

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4031

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3670

0.1ml

Concentrate

E3672

0.5ml

E3674 E3671

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Placenta

Control Tissue: Placenta

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Placenta stained with anti-MMP-10 (Stromelysin-2) antibody E367

Human Placenta stained with anti-MMP-19 antibody E403

Product Description: MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. This antibody reacts with both latent and active MMP-10.

Product Description: MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP-19 is the target of autoantibodies in rheumatoid arthritis and SLE.

MMP-14/MT1-MMP Rabbit Polyclonal

MMP-23 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E4040

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human MMP-14 protein.

E4042

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human MMP-23 protein.

1.0ml

Concentrate

Application: IHC-P

E4044

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4041

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3860

0.1ml

Concentrate

E3862

0.5ml

E3864 E3861

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Placenta

Control Tissue: Placenta

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Placenta stained with anti-MMP-14/MT1-MMP antibody E386

Human Placenta stained with anti-MMP-23 antibody E404

Product Description: MMPs are a group of enzymes involved in matrix degradation. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively, contain a C-terminus transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MMP-14 cleaves progelatinase A (MMP-2) and progelatinase B to their active forms and it gets activated through the membrane plasmin cascade.

Product Description: MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPs and TIMPs play a significant role in regulating angiogenesis. MMP-23 transcripts have been detected in reproductive organs like ovary, testis and prostate. MMP-23 is inhibited by TIMP-1.

MMP-15/MT2-MMP Rabbit Polyclonal

MSH2 Rabbit Monoclonal

Clone: N/A

Catalog No.

Size Format

Clone: SP46

M3460

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from the catalytic domain of mouse MMP-15/MT2-MMP protein.

M3462

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human MSH2.

1.0ml

Concentrate

Application: IHC-P

M3464

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3461

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E4120

0.1ml

Concentrate

E4122

0.5ml

E4124 E4121

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Colon Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Placenta stained with anti-MMP-15/MT2-MMP antibody E412

Human Colon Carcinoma stained with anti-MSH2 antibody M346

Product Description: MMPs are a group of enzymes involved in matrix degradation. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively contain a C-terminus transmembrane domain anchoring them to the cell surface. MMP15 plays a minor role in activation of pro-MMP-2 to its active form in breast carcinomas. MMP14 and MMP15 can be coexpressed in advanced stages of breast carcinoma.

Product Description: MSH2 as a mismatch repair protein is involved in DNA repair. Mutations of MSH2 are found in approximately 50% of inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and cancers of the gastrointestinal, urological and female reproductive tracts.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

73


MSH2 Rabbit Polyclonal

MSH6 Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E18370

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human MSH2.

E18372

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human MSH6 protein.

1.0ml

Concentrate

Application: IHC-P

E18374

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18371

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17790

0.1ml

Concentrate

E17792

0.5ml

E17794 E17791

Clone: SPM525

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgG1/k

Control Tissue: Colon Carcinoma

Control Tissue: Colon Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Colon Carcinoma stained with anti-MSH2 antibody E1779

Human Colon Carcinoma stained with anti-MSH6 antibody E1837

Product Description: MSH2 as a mismatch repair protein is involved in DNA repair. Mutations of MSH2 are found in approximately 50% of inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and cancers of the gastrointestinal, urological and female reproductive tracts.

Product Description: MSH6 as a mismatch repair protein is central to mismatch DNA repair. Mutations of MSH6 are found in inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and cancers of the gastrointestinal, urological and female reproductive tracts.

MSH3 Rabbit Polyclonal

MSH6 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E17670

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human MSH3 protein.

E17672

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human MSH6 protein.

1.0ml

Concentrate

Application: IHC-P

E17674

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17671

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18940

0.1ml

Concentrate

E18942

0.5ml

E18944 E18941

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Rectal Carcinoma

Control Tissue: Colon Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Rectal Carcinoma stained with anti-MSH3 antibody E1894

Human Colon Carcinoma stained with anti-MSH6 antibody E1767

Product Description: MSH3 belongs to the MutS DNA mismatch repair family although its precise function in mammals is still unknown. Yeast MSH3 is thought to act in a complex with MSH2 to recognize DNA insertion/deletion mispairs.

Product Description: MSH6 as a mismatch repair protein is central to mismatch DNA repair. Mutations of MSH6 are found in inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and cancers of the gastrointestinal, urological and female reproductive tracts.

MSH6 Rabbit Monoclonal

mTOR Rabbit Polyclonal

Catalog No.

Size

Format

M3930

0.1ml

Concentrate

M3932

0.5ml

Concentrate

M3934

1.0ml

Concentrate

M3931

7.0ml

Ready-to-Use

Clone: SP93

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to internal region of human MSH6 protein.

E18590

0.1ml

Concentrate

E18592

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human mTOR protein.

E18594

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

E18591

7.0ml

Ready-to-Use

Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Rectal Carcinoma Cellular Localization: Nucleus

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Breast Carcinoma Cellular Localization: Cytoplasm, Membrane

Control Lysate: A431 Molecular Weight: 160 kDa Human Rectal Carcinoma stained with anti-MSH6 antibody M393

Product Description: MSH6 as a mismatch repair protein is central to mismatch DNA repair. Mutations of MSH6 are found in inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and cancers of the gastrointestinal, urological and female reproductive tracts.

74

Human Breast Carcinoma stained with anti-mTOR antibody E1859

Product Description: Mammalian target of rapamycin (mTOR) or FKBP12-rapamycin associated protein (FRAP) is a serine/threonine-protein kinase involved in cell cycle progression, DNA recombination, and DNA damage detection. The MTOR-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways. The binding of FRAP to FKBP12-rapamycin is correlated with the ability of these ligands to inhibit cell cycle progression.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies mTOR Rabbit Polyclonal

MUC-1/EMA/Epithelial Membrane Antigen Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E11190

0.1ml

Concentrate

Immunogen: Human breast cell line ZR-75-1.

Concentrate

Immunogen: Synthetic peptide derived from human mTOR.

E11192

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P

1.0ml

E11194

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E11191

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E18450

0.1ml

Concentrate

E18452

0.5ml

E18454 E18451

Isotype: Rabbit IgG

Clone: SPM492

Isotype: Mouse IgG1/k Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Membrane

Human Breast Carcinoma stained with anti-mTOR antibody E1845

Human Breast Carcinoma stained with anti-MUC-1/EMA/Epithelial Membrane Antigen antibody E1119

Product Description: Mammalian target of rapamycin (mTOR) or FKBP12-rapamycin associated protein (FRAP) is a serine/threonine-protein kinase involved in cell cycle progression, DNA recombination, and DNA damage detection. The MTOR-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways. The binding of FRAP to FKBP12-rapamycin is correlated with the ability of these ligands to inhibit cell cycle progression.

Product Description: MUC-1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.

MUC-1 Underglycosylated Mouse Monoclonal

Mucin 5AC Mouse Monoclonal

Clone: SPM493

Catalog No.

Size

Format

Concentrate

Immunogen: Human breast cell line ZR-75-1.

E11080

0.1ml

Concentrate

Concentrate

Application: IHC-P, WB

E11082

0.5ml

Concentrate

Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-Use

Species Reactivity: Human*

E11084

7.0ml

E11081

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E11200

0.1ml

E11202

0.5ml

E11204 E11201

Isotype: Mouse IgG1/k

Clone: 45M1

Species Reactivity: Human*

Control Tissue: Breast Carcinoma

Isotype: Mouse IgG1/k

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Membrane

Control Tissue: Stomach

Control Lysate: MCF7 Molecular Weight: 230 kDa Human Breast Carcinoma stained with anti-MUC-1 Underglycosylated antibody E1120

Product Description: Epithelial membrane antigen (EMA), or CA15-3, or polymorphic epithelial mucin (PEM), or sialomucin, or episialin, is a mucin-like glycoprotein. This antibody stains an underglycosylated MUC1 often present on carcinoma cells.

MUC-1/EMA/Epithelial Membrane Antigen Rabbit Polyclonal

Human Stomach stained with anti-Mucin 5AC antibody E1108

Product Description: Mucins are high molecular weight glycoproteins with 80% carbohydrate contents and the remaining 20% is constituted by protein core. Gastric mucin is found in normal human gastrointestinal tract. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Clone: N/A

Mucin 5AC Mouse Monoclonal Catalog No.

Size Format

Clone: SPM488

Concentrate

Immunogen: Synthetic peptide from the cytoplasmic tail of human MUC-1.

E11070

0.1ml

Concentrate

Concentrate

Application: IHC-P

Immunogen: Recombinant Mucin 5AC protein.

1.0ml

E11072

0.5ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

Application: IHC-P

E11074

1.0ml

Concentrate

Isotype: Rabbit IgG

E11071

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E3540

0.1ml

Concentrate

E3542

0.5ml

E3544 E3541

Control Tissue: Breast Carcinoma

Control Tissue: Stomach

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm, Membrane

Human Breast Carcinoma stained with anti-MUC-1/EMA/Epithelial Membrane Antigen antibody E354

Product Description: MUC-1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.

Isotype: Mouse IgG1/k

Human Stomach stained with anti-Mucin 5AC antibody E1107

Product Description: Mucins are high molecular weight glycoproteins with 80% carbohydrate contents and the remaining 20% is constituted by protein core. Gastric mucin is found in normal human gastrointestinal tract. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

75


Mucin-2/MUC-2 Mouse Monoclonal

Myeloperoxidase (MPO) Rabbit Monoclonal

Clone: SPM512

Catalog No.

Size

Format

M3720

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to a site on the MUC-2 glycoprotein.

M3722

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human myeloperoxidase protein.

1.0ml

Concentrate

Application: IHC-P

M3724

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3721

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16560

0.1ml

Concentrate

E16562

0.5ml

E16564 E16561

Clone: SP72

Species Reactivity: Human*

Isotype: Mouse IgG1

Isotype: Rabbit IgG

Control Tissue: Small Intestine

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Human Small Intestine stained with anti-Mucin-2/MUC-2 antibody E1656

Human Tonsil stained with anti-Myeloperoxidase (MPO) antibody M372

Product Description: Secreted epithelial mucins are large macromolecules which exhibit extreme polydispersity. Mucin-2 is the major intestinal mucin. O-glycans are attached to MUC-2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins.

Product Description: Myeloperoxidase (MPO) is an important enzyme used by granulocytes during phagocytic lysis. In normal tissues and in a variety of myeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic types exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is postive in myeloid, but negative in lymphoid leukemias.

MUM1/IRF4 Rabbit Monoclonal

Myeloperoxidase (MPO) Rabbit Polyclonal

Clone: SP114

Catalog No.

Size

Format

E17710

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide near C-terminus of human MUM1/IRF4.

E17712

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human myeloperoxidase protein.

1.0ml

Concentrate

Application: IHC-P, WB

E17714

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17711

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M4140

0.1ml

Concentrate

M4142

0.5ml

M4144 M4141

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Hodgkin’s Lymphoma

Control Tissue: Lymph Node, Spleen, Tonsil

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Control Lysate: Ramos Molecular Weight: ~51.7 kDa Human Hodgkin’s Lymphoma stained with anti-MUM1/IRF4 antibody M414

Human Lymph Node stained with anti-Myeloperoxidase (MPO) antibody E1771

Product Description: The MUM1 (Multiple Myeloma Oncogene 1) gene was originally identified because of its involvement in the t(6;14) translocation observed in multiple myeloma, which causes juxtaposition of the MUM1 gene to the Ig heavy-chain locus. MUM1 is expressed in a wide spectrum of lymphoid neoplasms but not in myeloid and extra-hematopoitic tumors. It is an excellent marker for Reed-Sternberg cells of classic Hodgkin’s disease.

Product Description: Myeloperoxidase (MPO) is an important enzyme used by granulocytes during phagocytic lysis. In normal tissues and in a variety of myeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic types exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is postive in myeloid, but negative in lymphoid leukemias.

MUM1/IRF4 Rabbit Polyclonal

Myoglobin Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E2990

0.1ml

Concentrate

Immunogen: Purified human myoglobin.

Concentrate

Immunogen: Recombinant protein encoding aa 144451 of human MUM1 protein.

E2992

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P, WB

1.0ml

E2994

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2991

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E18350

0.1ml

Concentrate

E18352

0.5ml

E18354 E18351

Isotype: Rabbit IgG Control Tissue: Hodgkin’s Lymphoma Cellular Localization: Cytoplasm, Nucleus

Clone: N/A

Isotype: Rabbit IgG Control Tissue: Skeletal Muscle Cellular Localization: Cytoplasm Control Lysate: Human Skeletal Muscle Molecular Weight: 17 kDa

Human Hodgkin’s Lymphoma stained with anti-MUM1/IRF4 antibody E1835

Product Description: The MUM1 (Multiple Myeloma Oncogene 1) gene was originally identified because of its involvement in the t(6;14) translocation observed in multiple myeloma, which causes juxtaposition of the MUM1 gene to the Ig heavy-chain locus. MUM1 is expressed in a wide spectrum of lymphoid neoplasms but not in myeloid and extra-hematopoitic tumors. MUM1 is a valuable marker for understanding and characterizing histogenesis of B-cell lymphomas.

76

Human Skeletal Muscle stained with anti-Myoglobin antibody E299

Product Description: Myoglobin is a cytoplasmic, single chain polypeptide of 153 amino acids and contains a single heme group. Myoglobin is found in skeletal and cardiac muscle but not in smooth muscle and functions as an oxygen transporting pigment. Upon damage to the muscle cell due to infarction of a coronary artery, neurological trauma, infection of tumor processes, myoglobin escapes to the environment.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies NCK2 Rabbit Polyclonal

Nestin Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18750

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 119-276 of human NCK2.

E18752

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human nestin protein.

1.0ml

Concentrate

Application: IHC-P

E18754

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18751

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E11040

0.1ml

Concentrate

E11042

0.5ml

E11044 E11041

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Tonsil

Control Tissue: Liver Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-NCK2 antibody E1104

Human Liver Carcinoma stained with anti-Nestin antibody E1875

Product Description: The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.

Product Description: Nestin is a class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Nephrin Rabbit Polyclonal

Nestin Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18610

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human nephrin.

E18612

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human nestin protein.

1.0ml

Concentrate

Application: IHC-P

E18614

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18611

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18160

0.1ml

Concentrate

E18162

0.5ml

E18164 E18161

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Kidney

Control Tissue: Kidney, Liver Carcinoma

Cellular Localization: Membrane

Cellular Localization: Cytoplasm

Human Kidney stained with anti-Nephrin antibody E1816

Human Liver Carcinoma stained with anti-Nestin antibody E1861

Product Description: Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Three novel podocyte proteins, podocin, nephrin and alpha actinin 4 have been identified. The role of nephrin in anti-apoptotic activity in podocyte is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier.

Product Description: Nestin is a class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Nestin Rabbit Monoclonal

Neuron Specific Enolase (NSE) Rabbit Polyclonal

Catalog No.

Size

Format

M4030

0.1ml

Concentrate

M4032

0.5ml

Concentrate

M4034

1.0ml

Concentrate

M4031

7.0ml

Ready-to-Use

Clone: SP103

Catalog No.

Size Format

Clone: N/A

Immunogen: Synthetic peptide corresponding to C-terminus of human nestin.

E3300

0.1ml

Concentrate

E3302

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human NSE.

E3304

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

E3301

7.0ml

Ready-to-Use

Species Reactivity: Human*

Species Reactivity: Human*, Mouse*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas

Control Tissue: Kidney

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm Control Lysate: HeLa Molecular Weight: 230 kDa Human Kidney stained with anti-Nestin antibody M403

Product Description: Nestin is a class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Human Pancreas stained with anti-Neuron Specific Enolase (NSE) antibody E330

Product Description: Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2-phospho-glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (α,β and γ). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

77


NFkB p50 Rabbit Polyclonal

Nitric Oxide Synthase-Inducible (iNOS) Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M4260

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from N-terminus of human NFκB p50.

M4262

0.5ml

Concentrate

Immunogen: Synthetic peptide near the C-terminus of mouse iNOS protein.

1.0ml

Concentrate

Application: IHC-P

M4264

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M4261

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3630

0.1ml

Concentrate

E3632

0.5ml

E3634 E3631

Clone: SP126

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate, Testis, Tonsil

Control Tissue: Lung

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Tonsil stained with anti-NFkB p50 antibody E363

Human Lung stained with anti-Nitric Oxide Synthase-Inducible (iNOS) antibody M426

Product Description: NF kappa B is a heterodimer consisting of a DNA binding subunit and a transactivating subunit (RelA). P50 subunit of NFkB has an isoform and two proteins of 105 and 100kDa respectively. The p50/p65 heterodimer is present in the cytoplasm in active form bound to its inhibitor, IkB. Upon stimulation of cells with stimuli like LPS, IL-1, IkB gets phosphorylated and degraded by proteosomes releasing the activated NFkB which goes to the nucleus inducing gene expression.

Product Description: Inducible NOS (iNOS) or macrophage NOS (mNOS) is calcium/ calmodulin independent and is expressed in activated macrophages and stimulated glial cells. NOS oxidizes a guanidine nitrogen of arginine, releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation, neurotransmission, anti-tumor and anti-pathogenic activities.

NFkB p65 Rabbit Polyclonal

Nitric Oxide Synthase-Inducible (iNOS) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3740

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal domain of human NFκB p65.

E3742

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of mouse i-NOS.

1.0ml

Concentrate

Application: IHC-P

E3744

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3741

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2750

0.1ml

Concentrate

E2752

0.5ml

E2754 E2751

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta, Prostate

Control Tissue: Lung

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm

Human Prostate stained with anti-NFkB p65 antibody E275

Human Lung stained with anti-Nitric Oxide Synthase-Inducible (iNOS) antibody E374

Product Description: NF kappa B is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit (RelA). The p50/p65 heterodimer is present in the cytoplasm in inactive form bound to its inhibitor, IκB. In unstimulated cells, nuclear localization signals present on p65 are masked by members of the IκB family of inhibitory proteins. Subsequent to cell stimulation, IκB undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway.

Product Description: NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells. This antibody reacts with iNOS.

Nitric Oxide Synthase-Endothelial (eNOS) Rabbit Polyclonal

Nitric Oxide Synthase-Universal (uNOS) Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E3930

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human e-NOS.

E3932

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of mouse iNOS and nNOS.

1.0ml

Concentrate

Application: IHC-P

E3934

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3931

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E4110

0.1ml

Concentrate

E4112

0.5ml

E4114 E4111

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Placenta

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Placenta stained with anti-Nitric Oxide Synthase-Endothelial (eNOS) antibody E411

Human Placenta stained with anti-Nitric Oxide Synthase-Universal (uNOS) antibody E393

Product Description: NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with eNOS.

78

Clone: N/A

Product Description: NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated, acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies NKX3.1 Rabbit Polyclonal

Occludin Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E17460

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human NKX3.1

E17462

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E17464

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17461

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E14100

0.1ml

Concentrate

E14102

0.5ml

E14104 E14101

Clone: N/A Immunogen: Synthetic peptide corresponding to C-terminus of human occludin. Application: IHC-P, WB Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate, Prostate Carcinoma

Control Tissue: Lung

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Membrane Control Lysate: Human Kidney Molecular Weight: 65 kDa

Human Prostate stained with anti-NKX3.1 antibody E1410

Human Lung stained with anti-Occludin antibody E1746

Product Description: The NKX3.1 gene is an androgen regulated prostate-specific homeobox gene, located on chromosome 8p21. In mice, the homologue NKX3.1 is exclusively expressed in prostate epithelium. In humans, NKX3.1 expression is also restricted to the prostate. Many data show that NKX3.1 expression is highly, but not exclusively, specific for the prostate.

Product Description: Occludin can exist in a variety of phosphorylated forms, ranging up to approximately 82kDa. This phosphorylation is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression. The level of occludin varies. In brain tissue, occludin is highly expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution.

Notch-1 Rabbit Polyclonal

OCT-1 Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

E3840

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human notch-1.

E3842

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human OCT-1.

1.0ml

Concentrate

Application: IHC-P

E3844

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3841

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E10860

0.1ml

Concentrate

E10862

0.5ml

E10864 E10861

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Ovarian Carcinoma

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Nucleus

Human Ovarian Carcinoma stained with anti-Notch-1 antibody E1086

Human Tonsil stained with anti-OCT-1 antibody E384

Product Description: The 280-330kDa Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for notch include Jagged, Jagged-2 and delta. Jagged can activate notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes.

Product Description: The POU dimer from the OCT-1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. OCT-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by OCT-2. The OCT-1 and OCT-2 map to human chromosomes 1q22-q23 and 19, respectively.

NRF2 Rabbit Polyclonal

OCT-2 Rabbit Polyclonal Clone: N/A

Catalog No.

Size Format

Clone: N/A

E4300

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human NRF2 protein.

E4302

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human OCT-2.

1.0ml

Concentrate

Application: IHC-P

E4304

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4301

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E12500

0.1ml

Concentrate

E12502

0.5ml

E12504 E12501

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta, Lung

Control Tissue: Tonsil

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Nucleus

Human Placenta stained with anti-NRF2 antibody E1250

Human Tonsil stained with anti-OCT-2 antibody E430

Product Description: NRF1 (also designated TCF11), NRF2 and NRF3 are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE (Maf recognition elements) sequences. NRF2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.

Product Description: OCT-2 is a transcription factor belonging to the POU homeodomain family that binds to the Ig gene octomer sites regulating B cell specific genes. OCT-2 protein expression is not restricted to B cells, although expression levels are much higher in these cells. OCT-2 expression is reported to be significantly greater in germinal center derived lymphomas, although other B cell lymphomas also display high levels of expression.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

79


OCT-3/OCT-4 Rabbit Polyclonal

p120 (catenin delta 1) Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

M3630

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human OCT-3/OCT-4 protein.

M3632

0.5ml

Concentrate

Immunogen: Synthetic peptide from C-terminus of human p120.

1.0ml

Concentrate

Application: IHC-P

M3634

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3631

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18280

0.1ml

Concentrate

E18282

0.5ml

E18284 E18281

Clone: SP63

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Seminoma

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Seminoma stained with anti-OCT-3/OCT-4 antibody E1828

Human Breast Carcinoma stained with anti-p120 (catenin delta 1) antibody M363

Product Description: OCT-3 (also known as OCT-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. OCT-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of OCT-3/4 is required to sustain stem cell renewal, and up or down regulation induce divergent developmental programs.

Product Description: Catenin delta 1 (p120) is an efficient tyrosine kinase substrate implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for the cell-adhesion properties of cadherins.

OCT-3/OCT-4 Rabbit Polyclonal

p120 (catenin delta 1) Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E17530

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human OCT-3/OCT-4 protein.

E17532

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human p120.

1.0ml

Concentrate

Application: IHC-P

E17534

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17531

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17720

0.1ml

Concentrate

E17722

0.5ml

E17724 E17721

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Seminoma

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Seminoma stained with anti-OCT-3/OCT-4 antibody E1772

Human Breast Carcinoma stained with anti-p120 (catenin delta 1) antibody E1753

Product Description: OCT-3 (also known as OCT-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. OCT-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of OCT-3/4 is required to sustain stem cell renewal, and up or down regulation induce divergent developmental programs.

Product Description: Catenin delta 1 (p120) is an efficient tyrosine kinase substrate implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for the cell-adhesion properties of cadherins.

Osteopontin Rabbit Polyclonal

p14ARF Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E4350

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal domain of human osteopontin.

E4352

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human p14ARF.

1.0ml

Concentrate

Application: IHC-P

E4354

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4351

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3280

0.1ml

Concentrate

E3282

0.5ml

E3284 E3281

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Lymphoma

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Breast Carcinoma stained with anti-Osteopontin antibody E328

Human Lymphoma stained with anti-p14ARF antibody E435

Product Description: Osteopontin is a 34kDa extracellular matrix protein with a cell binding domain. It was originally identified as a major component of the noncollagenous organic bone matrix, however, it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation.

80

Clone: N/A

Product Description: The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF binds to mdm2 and promotes the rapid degradation of mdm2 protein required for mdm2 modification and concurrent p53 stabilization and accumulation. This interaction is mediated by the exon 1beta-encoded N-terminal domain of p14ARF and a C-terminus region of mdm2.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies p27Kip1 Rabbit Polyclonal

p53 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2010

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human p27Kip1.

E2012

0.5ml

Concentrate

Immunogen: Recombinant human full length wild type p53 protein.

1.0ml

Concentrate

Application: IHC-P

E2014

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2011

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2600

0.1ml

Concentrate

E2602

0.5ml

E2604 E2601

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Prostate

Control Tissue: Colon Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Prostate stained with anti-p27Kip1 antibody E260

Human Colon Carcinoma stained with anti-p53 antibody E201

Product Description: This antibody recognizes a protein of 27kDa, identified as p27Kip1. p27Kip1 is a candidate tumor suppressor gene and functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest.

Product Description: p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNA damage and possibly facilitates repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

p300/CBP Rabbit Polyclonal

p53 Mouse Monoclonal Clone: N/A

Catalog No.

Size

Format

E16570

0.1ml

Concentrate

Immunogen: Recombinant human p53 protein.

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human p300.

E16572

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P

1.0ml

E16574

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E16571

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E14070

0.1ml

Concentrate

E14072

0.5ml

E14074 E14071

Isotype: Rabbit IgG

Clone: SPM514

Isotype: Mouse IgG2a/k Control Tissue: Colon Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Breast Carcinoma stained with anti-p300/CBP antibody E1407

Human Colon Carcinoma stained with anti-p53 antibody E1657

Product Description: The p300-CBP is composed of two transcriptional co-activating proteins with similar structures: p300 and CBP. Both p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes such as cAMP-regulated enhancer (CRE). Both contain five protein interaction domains: the nuclear receptor interaction domain, the CREB-MYB interaction domain, the cysteine/histidine regions and the interferon binding domain.

Product Description: p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNA damage and possibly facilitates repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

p53 Rabbit Monoclonal

p57** Rabbit Monoclonal Clone: SP5

Catalog No.

Size Format

Clone: SP118

M4180

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant human full length wild type p53 protein.

M4182

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to an internal region of human p57 protein.

1.0ml

Concentrate

Application: IHC-P

M4184

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M4181

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

M3050

0.1ml

Concentrate

M3052

0.5ml

M3054 M3051

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Placenta

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Colon Carcinoma stained with anti-p53 antibody M305

Human Placenta stained with anti-p57 antibody M418

Product Description: p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNA damage and possibly facilitates repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Product Description: Cyclin-dependent kinase inhibitor 1C (CDKN 1C or p57/Kip2) is a nuclear protein and a potent inhibitor for several G1 cyclin/Cdk complexes. P57 inhibits cell proliferation and the mutation of p57 gene can lead to uncontrollable cell growth and malignancy such as hydatidiform mole.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

81


p57 Rabbit Polyclonal

p70S6k (Phospho T421/S424) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18500

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human p57/kip2 protein.

E18502

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E18504

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18501

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18990

0.1ml

Concentrate

E18992

0.5ml

E18994 E18991

Clone: N/A Immunogen: Synthetic peptide derived from the region of human p70S6K that contains phosphorylated threonine 421 and serine 424. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Hydatid Mole, Placenta Cellular Localization: Nucleus

Control Tissue: Alzheimer’s Brain, Breast Carcinoma

Human Hydatid Mole stained with anti-p57 antibody E1899

Human Alzheimer’s Brain stained with anti-p70S6k (Phospho T421/S424) antibody E1850

Cellular Localization: Cytoplasm

Product Description: p57/Kip 2 (or CDKN 1C) is a potent tight binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57/Kip 2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm’s tumor, and Beckwith Wiedemann syndrome (BWS, a cancer syndrome) making p57/Kip 2 a tumor suppressor candidate.

Product Description: Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka) and S6K2 (S6Kb). The S6K1 isoform has two splicing forms: p70S6K or p70S6K1 with a molecular mass of 70kDa and the second isoform, p85S6K or p85S6K1 with a molecular mass of 85kDa. Both p70 and p85 S6K1 are activated by multiple S/T phosphorylations in response to various mitogenic stimuli and nutrients. This antibody detects p70S6K only when phosphorylated at T421/S424.

p70S6k Rabbit Polyclonal

PAK alpha Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E12550

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human p70S6k protein.

E12552

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 525-545 of rat PAK alpha.

1.0ml

Concentrate

Application: IHC-P

E12554

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E12551

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18640

0.1ml

Concentrate

E18642

0.5ml

E18644 E18641

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Nucleus

Human Breast Carcinoma stained with anti-p70S6k antibody E1864

Human Breast Carcinoma stained with anti-PAK alpha antibody E1255

Product Description: Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka) and S6K2 (S6Kb). The S6K1 isoform has two splicing forms: p70S6K or p70S6K1 with a molecular mass of 70kDa and the second isoform, p85S6K or p85S6K1 with a molecular mass of 85kDa. Both, p70 and p85 S6K1 are activated by multiple S/T phosphorylations in response to various mitogenic stimuli and nutrients.

Product Description: Alpha PAK p68, beta PAK p65 and gamma PAK p62 are three isoforms of serine/threonine kinases. These three isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation.

p70S6k (Paired 421/424) Rabbit Polyclonal

Parathyroid Hormone Rabbit Polyclonal

Catalog No.

Size

Format

E18510

0.1ml

Concentrate

E18512

0.5ml

Concentrate

E18514

1.0ml

Concentrate

E18511

7.0ml

Ready-to-Use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide derived from the region of human p70S6K that contains non-phosphorylated threonine 421 and serine 424.

E18090

0.1ml

Concentrate

E18092

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human parathyroid hormone.

E18094

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P

E18091

7.0ml

Ready-to-Use

Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Breast Carcinoma Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-p70S6k (Paired 421/424) antibody E1851

Product Description: Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka) and S6K2 (S6Kb). The S6K1 isoform has two splicing forms: p70S6K or p70S6K1 with a molecular mass of 70kDa and the second isoform, p85S6K or p85S6K1 with a molecular mass of 85kDa. Both p70 and p85 S6K1 are activated by multiple S/T phosphorylations in response to various mitogenic stimuli and nutrients. This antibody detects p70S6K that is non-phosphorylated at T421/S424.

82

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Parathyroid Gland Cellular Localization: Cytoplasm

Human Parathyroid Gland stained with anti-Parathyroid Hormone antibody E1809

Product Description: Parathyroid hormone (PTH) is secreted by parathyroid cells. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism. Most PTH is secreted in its intact, mature 9.5kDa form, amino acids 1-84; however, it can also be secreted as N-terminal truncated fragments or C-terminus fragments after intracellular degradation, as in case of hypercalcemia.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Parathyroid Hormone Receptor 1 Rabbit Polyclonal Catalog No.

Size

Format

E17890

0.1ml

Concentrate

E17892

0.5ml

Concentrate

E17894

1.0ml

Concentrate

E17891

7.0ml

Ready-to-Use

PAX-5 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to N-terminus of human parathyroid hormone receptor type 1.

E4390

0.1ml

Concentrate

E4392

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PAX-5 protein.

E4394

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P

E4391

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Parathyroid Gland

Cellular Localization: Nucleus

Cellular Localization: Membrane

Human Parathyroid Gland stained with anti-Parathyroid Hormone Receptor 1 antibody E1789

Human Tonsil stained with anti-PAX-5 antibody E439

Product Description: Two human parathyroid hormone (hPTH) receptors are known: one that binds both hPTH and a PTH-related protein called PTHrP (the Type I receptor); and one that only binds hPTH (the Type II receptor).

Product Description: PAX-5 is a B cell specific activator protein. In the early stages of B cell development, PAX-5 influences the expression of several B cell specific genes, such as CD19 and CD20. PAX-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells. PAX-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis and then shifts to the fetal liver. This indicates that PAX-5 is important in B cell and neuronal development.

Parkin Rabbit Polyclonal

Paxillin Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E13870

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human parkin protein.

E13872

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 356-558 of human paxillin.

1.0ml

Concentrate

Application: IHC-P

E13874

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E13871

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3470

0.1ml

Concentrate

E3472

0.5ml

E3474 E3471

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Alzheimer’s Brain

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Alzheimer’s Brain stained with anti-Parkin antibody E347

Human Breast Carcinoma stained with anti-Paxillin antibody E1387

Product Description: Parkin has been mapped to chromosome 6 and encodes a protein of 465 amino acids with similarity to ubiquitin at the amino terminus and a RINGfinger motif at the carboxyl terminus. Parkinson’s disease is the second most common neurodegenerative disease following Alzheimer’s. The characteristic movement disorder results from dysfunction and neuron loss in the substantia nigra. Deletions in Parkin have been associated with autosomal-recessive juvenile parkinsonism.

Product Description: Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as SRC, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and CRK through its SH2 domain. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

PAX-5 Rabbit Monoclonal

PCNA Rabbit Polyclonal Clone: SP34

Catalog No.

Size Format

Clone: N/A

E2850

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PAX-5 protein.

E2852

0.5ml

Concentrate

Immunogen: Recombinant human PCNA protein.

1.0ml

Concentrate

Application: IHC-P

E2854

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2851

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

M3340

0.1ml

Concentrate

M3342

0.5ml

M3344 M3341

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Small Intestine, Tonsil

Cellular Localization: Nucleus

Cellular Localization: Nucleus Control Lysate: 293T Molecular Weight: 30 kDa

Human Tonsil stained with anti-PAX-5 antibody M334

Product Description: PAX-5 is a B cell specific activator protein. In the early stages of B cell development, PAX-5 influences the expression of several B cell specific genes, such as CD19 and CD20. PAX-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells. PAX-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis and then shifts to the fetal liver. This indicates that PAX-5 is important in B cell and neuronal development.

Human Tonsil stained with anti-PCNA antibody E285

Product Description: Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

83


PD-1/CD279 Rabbit Polyclonal

PDGFR alpha Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E2690

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human CD279 protein.

E2692

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PDGFR alpha.

1.0ml

Concentrate

Application: IHC-P

E2694

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2691

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18660

0.1ml

Concentrate

E18662

0.5ml

E18664 E18661

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Kidney, Ovarian Carcinoma, Skin

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane

Human Tonsil stained with anti-PD-1/CD279 antibody E1866

Human Ovarian Carcinoma stained with anti-PDGFR alpha antibody E269

Product Description: CD279, also known as Programmed Cell Death 1 (PD-1), is an immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8 thymocytes, and on activated T and B cells. It is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PDL1 (also known as B7H1) and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.

Product Description: PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta, coded by two different genes. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons. This antibody is specific for PDGFR alpha and does not react with PDGFR beta.

PD-1/CD279 Rabbit Polyclonal

PDGFR beta Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E2730

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human PD-1/CD279 protein.

E2732

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PDGFR beta.

1.0ml

Concentrate

Application: IHC-P

E2734

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2731

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18630

0.1ml

Concentrate

E18632

0.5ml

E18634 E18631

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lymph Node, Tonsil

Control Tissue: Kidney, Ovarian Carcinoma, Skin

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Membrane

Human Lymph Node stained with anti-PD-1/CD279 antibody E1863

Human Ovarian Carcinoma stained with anti-PDGFR beta antibody E273

Product Description: CD279, also known as Programmed Cell Death 1 (PD-1), is an immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8 thymocytes, and on activated T and B cells. It is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PDL1 (also known as B7H1) and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.

Product Description: PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta, coded by two different genes. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons. This antibody is specific for PDGFR beta and does not react with PDGFR alpha.

PDCD6 Rabbit Polyclonal

PDK-1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18410

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human PDCD6 protein.

E18412

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from the human PDK-1.

1.0ml

Concentrate

Application: IHC-P

E18414

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18411

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17690

0.1ml

Concentrate

E17692

0.5ml

E17694 E17691

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm

Human Lung Carcinoma stained with anti-PDCD6 antibody E1769

Human Breast Carcinoma stained with anti-PDK-1 antibody E1841

Product Description: This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant.

84

Clone: N/A

Product Description: Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways, including the activation of Akt and PKC isoenzyme p70S6 kinase and RSK. Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies PDS-1/Securin Rabbit Polyclonal

Phosphotyrosine Mouse Monoclonal

Clone: N/A

Catalog No.

Size

Format

E3080

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human PDS-1 protein.

E3082

0.5ml

Concentrate

Immunogen: Phosphotyrosine conjugated to protein carriers.

1.0ml

Concentrate

Application: IHC-P

E3084

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3081

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18530

0.1ml

Concentrate

E18532

0.5ml

E18534 E18531

Clone: SPM102

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Mouse IgG1

Control Tissue: Rectal Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Rectal Carcinoma stained with anti-PDS-1/Securin antibody E1853

Human Breast Carcinoma stained with anti-Phosphotyrosine antibody E308

Product Description: Securin is an anaphase inhibitor and plays an important role in DNA damage and spindle check point pathways. It is targeted for degradation by ubiquitination mediated by a ubiquitin ligase - anaphase promoting complex (APC). Securin is highly expressed in various human tumors, including pituitary, adrenal, kidney, liver, and ovarian tumors, and in cell lines derived from pituitary tumors, breast tumors, endometrial tumors, and ovarian tumors.

Product Description: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. This antibody is specific to phosphotyrosine and shows no cross-reaction with other phosphoamino acids.

Peripheral Myelin Protein (PMP-22) Rabbit Polyclonal

PI3K p110beta Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E18210

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from near C-terminus of human PMP-22.

E18212

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human PI3k p110beta.

1.0ml

Concentrate

Application: IHC-P

E18214

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18211

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3520

0.1ml

Concentrate

E3522

0.5ml

E3524 E3521

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas

Control Tissue: Breast Carcinoma, Placenta

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Pancreas stained with anti-Peripheral Myelin Protein (PMP-22) antibody E352

Human Breast Carcinoma stained with anti-PI3K p110beta antibody E1821

Product Description: PMP-22 (Peripheral Myelin Protein) is a glycoprotein expressed in the compact myolin of the peripheral nervous system. In the peripheral nervous system, PMP-22 is produced by myelinating Schwann cells and is coexpressed with the genes for myelin basic protein (MBP) and protein P0 during nerve development and regeneration. Alterations in the level of this protein cause several genetic human diseases.

Product Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol. The enzyme comprises a 110kDa catalytic subunit and a regulatory subunit of either 85, 55, or 50kDa. The loss of p110 beta decreases tumorigenesis in a mouse cancer model with lipid phosphatase PTEN deficiency. This antibody is specific to PI3K p110beta.

Phospho-Ser/Thr/Tyr Mouse Monoclonal

PI3K p85 Rabbit Monoclonal

Clone: SPM101

Catalog No.

Size

Format

M3620

0.1ml

Concentrate

Concentrate

Immunogen: Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers.

M3622

0.5ml

Concentrate

Immunogen: Synthetic peptide from N-terminus of human PI3K p85.

1.0ml

Concentrate

Application: WB

M3624

1.0ml

Concentrate

Application: IHC-P

N/A

N/A

Species Reactivity: Human*

M3621

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3070

0.1ml

Concentrate

E3072

0.5ml

E3074 N/A

Clone: SP62

Species Reactivity: Human*

Isotype: Mouse IgG1

Isotype: Rabbit IgG

Control Tissue: N/A

Control Tissue: Placenta

Cellular Localization: N/A

Cellular Localization: Cytoplasm

Control Lysate: Jurkat Molecular Weight: 20 KDa N/A E307

Product Description: This antibody recognizes serine, threonine, and tyrosine phosphorylated proteins as determined by ELISA using phosphorylated peptides. Reactivity has also been shown with phosphorylated proteins in western blots.

Human Placenta stained with anti-PI3K p85 antibody M362

Product Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110kDa catalytic subunit and a regulatory subunit of either 85, 55, or 50kDa. This antibody is specific to PI3K p85 which is a 85kDa regulatory subunit.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

85


PI3K p85 Rabbit Polyclonal

Placental Lactogen (hPL) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2980

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human PI3K p85.

E2982

0.5ml

Concentrate

Immunogen: Purified human placental lactogen.

1.0ml

Concentrate

Application: IHC-P

E2984

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2981

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18200

0.1ml

Concentrate

E18202

0.5ml

E18204 E18201

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Placenta

Control Tissue: Placenta

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm Control Lysate: Human Placenta Molecular Weight: 21 kDa

Human Breast Carcinoma stained with anti-PI3K p85 antibody E1820

Human Placenta stained with anti-Placental Lactogen (hPL) antibody E298

Product Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110kDa catalytic subunit and a regulatory subunit of either 85, 55, or 50kDa. This antibody is specific to PI3K p85 which is a 85kDa regulatory subunit.

Product Description: Human placental lactogen (hPL) is known to originate in the syncytiotrophoblasts. Ultrastructural localization indicates that placental lactogen is stored in small morphologically distinct cytoplasmic granules within these cells and has a different secretory mechanism from other hormonal peptides such as human chorionic gonadotropin. The level of expression of placental lactogen through pregnancy increases gradually to term.

PIM-1 Rabbit Polyclonal

PLAP Rabbit Monoclonal Clone: SP15

Clone: N/A

Catalog No.

Size

Format

M3150

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human PIM-1 protein.

M3152

0.5ml

Concentrate

Immunogen: Recombinant protein encoding human placental alkaline phosphatase.

1.0ml

Concentrate

Application: IHC-P

M3154

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3151

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18710

0.1ml

Concentrate

E18712

0.5ml

E18714 E18711

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Bone Marrow

Control Tissue: Placenta, Seminoma

Cellular Localization: Cytoplasm, Membrane, Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Bone Marrow stained with anti-PIM-1 antibody E1871

Human Seminoma stained with anti-PLAP antibody M315

Product Description: PIM-1 is a member of the serine/threonine kinase PIM oncogene family. PIM-1 has been implicated in lymphomagenesis, cell proliferation, apoptosis, differentiation and tumourigenesis. The PIM-1 protein kinase is upregulated in prostate cancer.

Product Description: This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is expressed in testicular germ cell tumors. Unlike germ cell tumors, PLAPpositive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

PIM-2 Rabbit Polyclonal

PLAP Rabbit Polyclonal Catalog No.

Size

Format

E2040

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human PIM-2 protein.

E2042

0.5ml

Concentrate

Immunogen: Recombinant protein encoding human placental alkaline phosphatase.

1.0ml

Concentrate

Application: IHC-P

E2044

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2041

7.0ml

Ready-to-Use

Size

Format

E18800

0.1ml

Concentrate

E18802

0.5ml

E18804 E18801

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: B Cell Lymphoma

Control Tissue: Placenta, Seminoma

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm, Membrane

Human B Cell Lymphoma stained with anti-PIM-2 antibody E1880

Human Seminoma stained with anti-PLAP antibody E204

Product Description: PIM-2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM-1 and PIM-3. Expression of this protein is regulated by hematopoietic cytokines. PIM-2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. PIM-2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3.

86

Clone: N/A

Clone: N/A

Catalog No.

Product Description: This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is expressed in testicular germ cell tumors. Unlike germ cell tumors, PLAPpositive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies PLC beta 1 Rabbit Polyclonal

PML Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E12400

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human PLC beta 1.

E12402

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to near N-terminus of human PML.

1.0ml

Concentrate

Application: IHC-P

E12404

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E12401

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E14110

0.1ml

Concentrate

E14112

0.5ml

E14114 E14111

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Lymph Node

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Nucleus

Human Brain stained with anti-PLC beta 1 antibody E1411

Human Lymph Node stained with anti-PML antibody E1240

Product Description: Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes such as PLC β1a and PLC β1b. PLC β1a is preferentially expressed in the cytosol, whereas PLC β1b is predominantly localized in the nuclei.

Product Description: The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The t(15;17) (q22;q11.2-q12) chromosomal translocation of the retinoic acid receptor alpha gene occurs in virtually all cases of acute promyelocytic leukemia. Myeloid precursor cells expression the PML/RAR alpha chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis.

PLC gamma 2 Rabbit Polyclonal

PMS2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E18560

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PLC gamma 2 protein.

E18562

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PMS2 protein.

1.0ml

Concentrate

Application: IHC-P

E18564

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18561

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E12470

0.1ml

Concentrate

E12472

0.5ml

E12474 E12471

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Hodgkin’s Lymphoma

Control Tissue: Rectal Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Nucleus

Human Hodgkin’s Lymphoma stained with anti-PLC gamma 2 antibody E1247

Human Rectal Carcinoma stained with anti-PMS2 antibody E1856

Product Description: Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5 bisphosphate. There are many mammalian PLC isozymes, including PLC B1, PLC B2, PLC B3, PLCB4, PLCy1, PLCy2, PLC 81, PLC82 and PLCe with molecular weights ranging from 85 to 150kDa.

Product Description: Mismatch DNA repair in bacteria is carried out by the MutL, MutH, and MutS proteins. The MutS protein initially binds to mismatched DNA. This is followed by binding of the MutH endonuclease and MutL to form a complex that carries out excision repair. The hMSH2 gene specifies a MutS homologue whereas hMLH1, hPMS1, and hPMS2 encode MutL homologs. Mutations in these genes are associated with hereditary nonpolyposis colon cancer (HNPCC).

Pleiotrophin Rabbit Polyclonal

Podocin Rabbit Polyclonal

Clone: N/A

Catalog No.

Size Format

Clone: N/A

E18150

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human pleiotrophin protein.

E18152

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E18154

1.0ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human podocin.

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18151

7.0ml

Ready-to-Use

Application: IHC-P, WB

Catalog No.

Size

Format

E18820

0.1ml

Concentrate

E18822

0.5ml

E18824 E18821

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain, Colon Carcinoma

Control Tissue: Kidney

Cellular Localization: Cytoplasm

Cellular Localization: Membrane Control Lysate: 293T Molecular Weight: 42/34 kDa

Human Brain stained with anti-Pleiotrophin antibody E1882

Product Description: Pleiotrophin (PTN) is a secreted heparin and chondroitin-binding protein. It is a regulatory cytokine critical to the early development of blood vessels and neurons. High PTN serum levels are associated with a variety of solid tumors such as multiple myeloma (MM). The amount of PTN is correlated with the MM disease status and response to treatment. The expression of this protein is very limited in normal adult tissues.

Human Kidney stained with anti-Podocin antibody E1815

Product Description: Podocin (NPHS2 protein) is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. Podocin is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

87


Poly ADP-Ribose Polymerase (PARP) Rabbit Polyclonal

Progesterone Receptor (PR) Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

M3420

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human PARP protein.

M3422

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 412526 of human progesterone receptor.

1.0ml

Concentrate

Application: IHC-P

M3424

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3421

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E4220

0.1ml

Concentrate

E4222

0.5ml

E4224 E4221

Clone: SP42

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Tonsil stained with anti-Poly ADP-Ribose Polymerase (PARP) antibody E422

Human Breast Carcinoma stained with anti-Progesterone Receptor (PR) antibody M342

Product Description: PARP uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death and is mediated by the caspase CPP32 protein.

Product Description: The progesterone receptor (PR) is an estrogen-regulated protein. It has been proposed that expression of PR determination indicates a responsive estrogen receptor (ER) pathway. A number of studies have shown that PR determination provides supplementary information to ER.

Potassium Channel Kv3.2 Rabbit Polyclonal

Progesterone Receptor (PR) Rabbit Monoclonal

Clone: N/A

Catalog No.

Size

Format

M3020

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from internal region of rat potassium channel Kv3.2.

M3022

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 412-526 of human progesterone receptor.

1.0ml

Concentrate

Application: IHC-P

M3024

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3021

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E10800

0.1ml

Concentrate

E10802

0.5ml

E10804 E10801

Clone: SP2

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Brain stained with anti-Potassium Channel Kv3.2 antibody E1080

Human Breast Carcinoma stained with anti-Progesterone Receptor (PR) antibody M302

Product Description: Potassium channels contribute to maintaining cell volume, membrane potential, neuronal excitability and the secretion of transmitters, salt and hormones. Two families of potassium channels have been identified. One family includes the inwardly rectifying potassium channels whereas, the other family includes: voltagesensing (KV); big conductance, calcium activated (BKca); and small conductance, calcium activated (SK) potassium channels.

Product Description: The progesterone receptor (PR) is an estrogen-regulated protein. It has been proposed that expression of PR determination indicates a responsive estrogen receptor (ER) pathway. A number of studies have shown that PR determination provides supplementary information to ER.

Presenilin Rabbit Polyclonal

Progesterone Receptor (PR) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2070

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human presenilin 1 protein.

E2072

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PgR.

1.0ml

Concentrate

Application: IHC-P

E2074

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2071

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3480

0.1ml

Concentrate

E3482

0.5ml

E3484 E3481

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Nucleus

Human Brain stained with anti-Presenilin antibody E348

Human Breast Carcinoma stained with anti-Progesterone Receptor (PR) antibody E207

Product Description: In addition to β-amyloid precursor protein (β-APP), three other gene products have been implicated in Familial Alzheimer’s Disease (FAD): presenilin 1 (PS-1), presenilin 2 (PS-2), and apolipoprotein E (apoE4). Mutations in the genes encoding β-APP, PS-1, and PS-2 are believed to contribute to presenile FAD.

88

Clone: N/A

Product Description: The progesterone receptor (PR) is an estrogen-regulated protein. It has been proposed that expression of PR determination indicates a responsive estrogen receptor (ER) pathway. A number of studies have shown that PR determination provides supplementary information to ER.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Prohibitin Rabbit Polyclonal

Prostate Specific Antigen (PSA) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E2960

0.1ml

Concentrate

Immunogen: Purified human PSA.

Concentrate

Immunogen: Synthetic peptide corresponding to near C-terminus of human prohibitin protein.

E2962

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P

1.0ml

E2964

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2961

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E17750

0.1ml

Concentrate

E17752

0.5ml

E17754 E17751

Isotype: Rabbit IgG

Clone: N/A

Isotype: Rabbit IgG Control Tissue: Prostate, Prostate Carcinoma

Control Tissue: Heart

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Heart stained with anti-Prohibitin antibody E1775

Human Prostate stained with anti-Prostate Specific Antigen (PSA) antibody E296

Product Description: Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin appears to be a very reliable marker of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Product Description: PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Prolactin Rabbit Polyclonal

Prostate Stem Cell Antigen (PSCA) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Purified human prolactin.

E3290

0.1ml

Concentrate

Concentrate

Application: IHC-P

E3292

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from extracellular region of human PSCA protein.

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

Ready-to-Use

Species Reactivity: Human*

E3294

7.0ml

E3291

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17490

0.1ml

E17492

0.5ml

E17494 E17491

Isotype: Rabbit IgG

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: Pituitary Cellular Localization: Cytoplasm

Control Tissue: Prostate, Prostate Carcinoma

Human Pituitary stained with anti-Prolactin antibody E1749

Human Prostate Carcinoma stained with anti-Prostate Stem Cell Antigen (PSCA) antibody E329

Cellular Localization: Cytoplasm, Membrane

Product Description: Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. Prolactin producing cells make up approximately 20% of the pituitary.

Product Description: PSCA (Prostate Stem Cell antigen) is a cell surface antigen, which is overexpressed in approximately 40% of primary prostate cancers and in as many as 100% of metastatic ones. PSCA is also overexpressed in a majority of transitional cell and pancreatic carcinomas.

Prostate Apoptosis Response 4 (PAR-4) Rabbit Polyclonal

Protein Gene Protein 9.5 (PGP9.5) Rabbit Polyclonal

Catalog No.

Size

Format

E3420

0.1ml

Concentrate

E3422

0.5ml

Concentrate

E3424

1.0ml

Concentrate

E3421

7.0ml

Ready-to-Use

Clone: N/A

Catalog No.

Size Format

Clone: N/A

Immunogen: Synthetic peptide derived from C-terminus of human prostate apoptosis response 4 protein.

E3340

0.1ml

Concentrate

E3342

0.5ml

Concentrate

Immunogen: Recombinant full length human protein gene product 9.5 protein.

E3344

1.0ml

Concentrate

Application: IHC-P

Application: IHC-P, WB

E3341

7.0ml

Ready-to-Use

Species Reactivity: Human*

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas, Small Intestine

Control Tissue: Heart

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm

Control Lysate: Human Brain Molecular Weight: 27 kDa

Human Heart stained with anti-Prostate Apoptosis Response 4 (PAR-4) antibody E342

Product Description: Prostate apoptosis response-4 (PAR-4) is a 38kDa protein originally identified as the product of a gene that is upregulated in prostate tumor cells undergoing apoptosis. It is a leucine zipper and death domain-containing protein whose levels increase in neurons undergoing apoptosis as a result of trophic factor withdrawal or exposure to oxidative and metabolic insults. PAR-4 levels are increased in the spinal cord specimens from patients with neuronal degeneration.

Human Pancreas stained with anti-Protein Gene Protein 9.5 (PGP9.5) antibody E334

Product Description: Protein gene product (PGP9.5) is a neuron specific protein distinct from neuron specific enolase. PGP9.5 is present in neurons, nerve fibers, neuroendocrine cells, renal tubules, spermatogonia, Leydig cells of the testis, ova and some cells of both the pregnant and non-pregnant corpus luteum.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

89


PSMA Rabbit Monoclonal

RAD51 Rabbit Polyclonal Clone: SP29

Catalog No.

Size

Format

E19100

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PSMA.

E19102

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human RAD51 protein.

1.0ml

Concentrate

Application: IHC-P

E19104

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E19101

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3290

0.1ml

Concentrate

M3292

0.5ml

M3294 M3291

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate Carcinoma

Control Tissue: Breast Carcinoma, Tonsil

Cellular Localization: Membrane

Cellular Localization: Nucleus

Human Prostate Carcinoma stained with anti-PSMA antibody M329

Human Breast Carcinoma stained with anti-RAD51 antibody E1910

Product Description: Prostate-specific membrane antigen (PMSA) is a type II transmembrane glycoprotein belonging to the M28 peptidase family. Three functionally distinct proteins are encoded including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate. PSMA is involved in prostate tumor progression.

Product Description: RAD51 is a member of the RAD51 protein family involved in repair of DNA double strand breaks. The protein is highly conserved in most eukaryotes from yeast to humans. It can interact with the ssDNA-binding protein RPA and Rad52, and is thought to play roles in homologous pairing and strand transfer of DNA. The interactions between Rad51 with BRCA1 and BRCA2 may be important for the cellular response to DNA damage. RAD51 is overexpressed in a variety of tumors.

PSMA Rabbit Polyclonal

RAN Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E12460

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human PSMA.

E12462

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 197-216 of human RAN protein.

1.0ml

Concentrate

Application: IHC-P

E12464

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E12461

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17330

0.1ml

Concentrate

E17332

0.5ml

E17334 E17331

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate Carcinoma

Control Tissue: Breast

Cellular Localization: Membrane

Cellular Localization: Nucleus

Human Prostate Carcinoma stained with anti-PSMA antibody E1733

Human Breast stained with anti-RAN antibody E1246

Product Description: Prostate-specific membrane antigen (PMSA) is a type II transmembrane glycoprotein belonging to the M28 peptidase family. Three functionally distinct proteins are encoded including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate. PSMA is involved in prostate tumor progression.

Product Description: The small Ras-related protein Ran, also called TC4, is a 24kDa, nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex.

RACK-1 Rabbit Polyclonal

RCAS1 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E16370

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human RACK-1 protein.

E16372

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human RCAS1.

1.0ml

Concentrate

Application: IHC-P

E16374

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E16371

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18910

0.1ml

Concentrate

E18912

0.5ml

E18914 E18911

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Prostate Carcinoma stained with anti-RACK-1 antibody E1891

Human Breast Carcinoma stained with anti-RCAS1 antibody E1637

Product Description: RACK-1 (receptor for activated C kinase 1) was identified through its binding to various PKC isoforms. Its main function is to recruit PKC and various other proteins to specific locations to form multiprotein complexes, mediating various signal pathways.

90

Clone: N/A

Product Description: RCAS-1 (Receptor binding cancer antigen expressed on SiSo cells) is a type III transmembrane protein mostly in the Golgi. It induces apoptosis of natural killer (NK)/T cells through activation of interleukin-1-beta converting enzyme (ICE)-like proteases. RCAS-1 is abundantly expressed in prostate cancer cells when compared to normal epithelial cells.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies RCC1 Rabbit Polyclonal

Retinoic X Receptor gamma Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E4330

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human RCC1.

E4332

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of mouse RXR gamma.

1.0ml

Concentrate

Application: IHC-P

E4334

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4331

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E13440

0.1ml

Concentrate

E13442

0.5ml

E13444 E13441

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Breast, Prostate Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus Control Lysate: NIH3T3 Molecular Weight: 60 kDa

Human Colon Carcinoma stained with anti-RCC1 antibody E1344

Human Prostate Carcinoma stained with anti-Retinoic X Receptor gamma antibody E433

Product Description: Regulator of Chromosome Condensation 1 (RCC-1) binds to chromatin and participates in the regulation of chromosome condensation in the S phase. RCC-1 interacts with GTP-binding nuclear protein Ran and catalyzes guanine nucleotide exchange. Both RCC-1 and Ran are components of an intrinsic checkpoint control that prevents the premature initiation of mitosis.

Product Description: Retinoids are metabolites of vitamin A and represent important signaling molecules during vertebrate development and tissue differentiation. Two families of retinoid receptors have been identified: retinoic acid receptors (RAR-α, RAR-β and RAR-γ); and retinoid X receptors (RXR-α, RXR-β and RXR-γ). RXRs are activated by 9-cis-retinoic acid, a stereo- and photoisomer of all-trans-RA expressed in vivo in both liver and kidney.

Retinoblastoma (Rb) Rabbit Polyclonal

RHO Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E17850

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human retinoblastoma.

E17852

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to human Rho protein.

1.0ml

Concentrate

Application: IHC-P

E17854

1.0ml

Concentrate

Application: WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

N/A

N/A

N/A

Catalog No.

Size

Format

E4310

0.1ml

Concentrate

E4312

0.5ml

E4314 E4311

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Colon Carcinoma

Control Tissue: N/A

Cellular Localization: Nucleus

Cellular Localization: N/A Control Lysate: MCF7 Molecular Weight: 21 kDa

Human Colon Carcinoma stained with anti-Retinoblastoma (Rb) antibody E431

N/A E1785

Product Description: Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth.

Product Description: The RHO proteins are members of the Ras superfamily of small GTP-binding proteins. The RHO proteins play an important role in the organization of actin cytoskeleton, cell adhesion, cell motility and smooth muscle contraction. The RHO proteins are also involved in the signal transduction pathways mediated by the actin cytoskeleton and the activation of the stress activated MAP kinase cascades.

Retinoic Acid Receptor beta Rabbit Polyclonal

ROC1 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size Format

Clone: N/A

E4190

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human RAR beta.

E4192

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human ROC1.

1.0ml

Concentrate

Application: IHC-P

E4194

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4191

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E4370

0.1ml

Concentrate

E4372

0.5ml

E4374 E4371

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Heart, Testis

Control Tissue: Tonsil

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Testis stained with anti-Retinoic Acid Receptor beta antibody E437

Human Tonsil stained with anti-ROC1 antibody E419

Product Description: Retinoids are metabolites of vitamin A and represent important signaling molecules during vertebrate development and tissue differentiation. Two families of retinoid receptors have been identified: retinoic acid receptors (RARs-α, -β and -γ); and retinoid X receptors (RXR-α, -β and -γ). RXRs have a high affinity for all-trans retinoic acids and belong to the same class of nuclear transcription factors such as thyroid hormone receptors and vitamin D3 receptor.

Product Description: ROC1 and ROC2 are the two highly conserved RING finger proteins that are homologous to APC11, a subunit of the anaphase-promoting complex (APC). ROC1 and ROC2 commonly interact with all cullins. ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1- and E2dependent manner. ROC1 is thought to play a unique role in the ubiquitination reaction by heterodimerizing with cullin1 to catalyze ubiquitin polymerization.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

91


RRM1 Rabbit Polyclonal

S6 Ribosomal Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E17780

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human RRM1.

E17782

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human S6 ribosomal.

1.0ml

Concentrate

Application: IHC-P

E17784

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17781

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18040

0.1ml

Concentrate

E18042

0.5ml

E18044 E18041

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm Control Lysate: NIH3T3 Molecular Weight: 32 kDa

Human Placenta stained with anti-RRMI antibody E1804

Human Breast Carcinoma stained with anti-S6 Ribosomal antibody E1778

Product Description: RRM1 (Ribonucleotide reductase M1 polypeptide) is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme which catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. It provides the precursors necessary for DNA synthesis. RRM1 is involved in carcinogenesis and tumor progression.

Product Description: 40S ribosomal protein S6 (also known as RPS6) is a 31kDa substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247. Hyperphosphorylation of RPS6 stimulates protein synthesis that mediates progression through the cell cycle.

S100 Rabbit Polyclonal

S6 Ribosomal (Paired 235/236) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18080

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding human S100 protein.

E18082

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E18084

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18081

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2140

0.1ml

Concentrate

E2142

0.5ml

E2144 E2141

Isotype: Rabbit IgG

Clone: N/A Immunogen: Synthetic peptide derived from the region of human S6 ribosomal that contains unphosphorylated serines 235 and 236. Application: IHC-P, WB Species Reactivity: Human*

Control Tissue: Melanoma, Schwannoma

Isotype: Rabbit IgG

Cellular Localization: Cytoplasm

Control Tissue: Breast Carcinoma Cellular Localization: Cytoplasm Control Lysate: 293T Molecular Weight: 32 kDa

Human Melanoma stained with anti-S100 antibody E214

Human Breast Carcinoma stained with anti-S6 Ribosomal (Paired 235/236) antibody E1808

Product Description: S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. The antibody to S100 stains schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells.

Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244->247. This antibody detects non-phosphorylated S6 ribosomal at 235/236 serine residue.

S100A4 Rabbit Polyclonal

S6 Ribosomal (Paired 240/244) Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E17770

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding full length human S100A4.

E17772

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P, WB

E17774

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17771

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E4440

0.1ml

Concentrate

E4442

0.5ml

E4444 E4441

Isotype: Rabbit IgG Control Tissue: Melanoma, Tonsil Cellular Localization: Cytoplasm Control Lysate: Human Placenta

Clone: N/A Immunogen: Synthetic peptide derived from the region of human S6 ribosomal that contains nonphosphorylated serines 240 and 244. Application: IHC-P Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Breast Carcinoma Cellular Localization: Cytoplasm

Molecular Weight: 12 kDa Human Tonsil stained with anti-S100A4 antibody E444

Product Description: S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph node.

92

Human Breast Carcinoma stained with anti-S6 Ribosomal (Paired 240/244) antibody E1777

Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244->247. Hyperphosphorylation of RPS6 stimulates protein synthesis. This antibody detects non-phosphorylated S6 ribosomal at 240/244 serine residue.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies S6 Ribosomal (Phospho S235/236) Rabbit Monoclonal Catalog No.

Size

Format

M3500

0.1ml

Concentrate

M3502

0.5ml

Concentrate

M3504

1.0ml

Concentrate

M3501

7.0ml

Ready-to-Use

S6 Ribosomal (Phospho S240/244) Rabbit Polyclonal

Clone: SP50

Catalog No.

Size

Format

Immunogen: Synthetic peptide derived from the region of human S6 ribosomal that contains phosphorylated serines 235 and 236.

E17760

0.1ml

Concentrate

E17762

0.5ml

Concentrate

E17764

1.0ml

Concentrate

Application: IHC-P

E17761

7.0ml

Ready-to-Use

Clone: N/A Immunogen: Synthetic peptide derived from the region of human S6 ribosomal that contains phosphorylated serines 240 and 244. Application: IHC-P

Species Reactivity: Human*

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-S6 Ribosomal (Phospho S235/236) antibody M350

Human Breast Carcinoma stained with anti-S6 Ribosomal (Phospho S240/244) antibody E1776

Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244->247. Hyperphosphorylation of RPS6 stimulates protein synthesis. This antibody detects S6 ribosomal only when it is phosphorylated at 235/236 serine residue.

Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244->247. Hyperphosphorylation of RPS6 stimulates protein synthesis. This antibody detects S6 Ribosomal only when it is phosphorylated at 240/244 serine residue.

S6 Ribosomal (Phospho S235/236) Rabbit Polyclonal

SHIP-1 Rabbit Polyclonal

Catalog No.

Size

Format

E18070

0.1ml

Concentrate

E18072

0.5ml

Concentrate

E18074

1.0ml

Concentrate

E18071

7.0ml

Ready-to-Use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide derived from the region of human S6 ribosomal that contains phosphorylated serines 235 and 236.

E14060

0.1ml

Concentrate

E14062

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human SHIP-1.

Application: IHC-P

E14064

1.0ml

Concentrate

Application: IHC-P

E14061

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Breast Carcinoma

Cellular Localization: Membrane

Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-S6 Ribosomal (Phospho S235/236) antibody E1807

Human Tonsil stained with anti-SHIP-1 antibody E1406

Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244->247. Hyperphosphorylation of RPS6 stimulates protein synthesis. This antibody detects S6 ribosomal only when it is phosphorylated at 235/236 serine residue.

Product Description: SHIP-1 p145 (SH2-containing-inositol phosphatase) contains two phosphotyrosine-binding domains (PTB), a unique amino terminal SH2 domain, and two highly conserved motifs found among inositol phosphate 5-phosphatases. SHIP-1 displays both phosphatidylinositol 3,4,5-triphosphate and inositol 1, 3, 4, 5 -tetrakisphosphate polyphosphate 5-phosphatase activity. Evidence suggests that SHIP-1 may modulate Ras signaling in addition to inositol signaling pathways.

S6 Ribosomal (Phospho S240/244) Rabbit Monoclonal

SH-PTP2 Rabbit Polyclonal

Catalog No.

Size

Format

M3450

0.1ml

Concentrate

M3452

0.5ml

Concentrate

M3454

1.0ml

Concentrate

M3451

7.0ml

Ready-to-Use

Clone: SP45

Catalog No.

Size Format

Clone: N/A

Immunogen: Synthetic peptide derived from the region of human S6 ribosomal that contains phosphorylated serines 240 and 244.

E12440

0.1ml

Concentrate

E12442

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to aa 576-593 of human SH-PTP2 protein.

Application: IHC-P

E12444

1.0ml

Concentrate

Application: IHC-P

E12441

7.0ml

Ready-to-Use

Species Reactivity: Human*

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Bone Marrow

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-S6 Ribosomal (Phospho S240/244) antibody M345

Product Description: 40S ribosomal protein S6 (RPS6) is a 31kDa substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244->247. This antibody detects S6 ribosomal only when it is phosphorylated at 240/244 serine residue.

Human Bone Marrow stained with anti-SH-PTP2 antibody E1244

Product Description: The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). SH-PTP1 is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH-PTP2 works on the downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

93


SKP1 p19 Rabbit Polyclonal Catalog No.

Size

Format

E14120

0.1ml

Concentrate

E14122

0.5ml

Concentrate

E14124

1.0ml

Concentrate

E14121

7.0ml

Ready-to-Use

SLIT2 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to C-terminus of human SKP1 p19.

E19050

0.1ml

Concentrate

E19052

0.5ml

Concentrate

Immunogen: Synthetic peptide near N-terminus of human SLIT2.

E19054

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

E19051

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Fetal Kidney, Prostate Carcinoma

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus Control Lysate: NIH3T3 Molecular Weight: 19 kDa

Human Prostate Carcinoma stained with anti-SLIT2 antibody E1905

Human Breast Carcinoma stained with anti-SKP1 p19 antibody E1412

Product Description: SKP1 p19 and SKP2 p45 are two cyclin A-CDK2 complex binding proteins participating in cell cycle regulation. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (CDK2). Although the Skps (S phase kinaseassociated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex.

Product Description: Slit homolog 2 proteins (SLIT2) are ligands for the Roundabout (Robo) receptors and act as molecule guidance cues in cell migration. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. SLIT2 is a target for repression by EZH2 and is up-regulated in prostate cancer.

SKP2 p45 Rabbit Polyclonal

SMA Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

E19220

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant protein encoding aa 221-425 of human SKP2 alpha.

E19222

0.5ml

Concentrate

Immunogen: Synthetic peptide near the N-terminus of human smooth muscle actin-alpha.

1.0ml

Concentrate

Application: IHC-P

E19224

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E19221

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E13880

0.1ml

Concentrate

E13882

0.5ml

E13884 E13881

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate

Control Tissue: Colon, Small Intestine

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm

Human Prostate stained with anti-SKP2 p45 antibody E1388

Human Small Intestine stained with anti-SMA antibody E1922

Product Description: SKP2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the cyclin A-CDK2 complex. SKP2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. SKP2 is widely expressed in normal and cancer tissues such as tonsil, placenta and cancers from colon, prostate, pancreas, skin, lung, breast, ovarian, and endometrium. SKP2 plays a role in oncogenesis, particularly in lymphomas and breast cancers.

Product Description: Smooth muscle actin-alpha is a cytoskeleton protein in smooth muscle cells and their derived tumors such as leiomyoma and leiomyosarcoma. It is also expressed in myoepithelial cells of the breast and salivary gland, but not in fibroblasts, striated muscle, and myocardium.

SLIT2 Rabbit Monoclonal

SMAD3 Rabbit Polyclonal Catalog No.

Size

Format

E11250

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human SLIT2 protein.

E11252

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human SMAD3.

1.0ml

Concentrate

Application: IHC-P

E11254

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*, Mouse*

E11251

7.0ml

Ready-to-Use

Size

Format

M4220

0.1ml

Concentrate

M4222

0.5ml

M4224 M4221

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Prostate Adenocarcinoma

Control Tissue: Breast, Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus

Human Prostate Adenocarcinoma stained with anti-SLIT2 antibody M422

Human Breast Carcinoma stained with anti-SMAD3 antibody E1125

Product Description: SLIT2 are ligands for Robo receptors and play a vital role for axonal migration/navigation during neural development. A number of cleavage products through alternate splicing are reported in the literature for SLIT2 proteins. The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. SLIT2 protein is expressed in tissues such as fetal lung, kidney, and adult spinal cord.

94

Clone: N/A

Clone: SP122

Catalog No.

Product Description: SMAD proteins mediate signaling events leading to transcriptional activation initiated by members of the TGF-beta superfamily. Biological activities mediated by smads include cell growth and morphogenesis, development, and immune response. A reduction in SMAD2/3 signaling enhances tumorigenesis, but also suppresses metastasis of breast cancer cell lines.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies SMRTe Rabbit Polyclonal

SOCS-6 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E16780

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to aa 994-1005 of human SMRTe protein.

E16782

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human SOCS-6 protein.

1.0ml

Concentrate

Application: IHC-P

E16784

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E16781

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E10990

0.1ml

Concentrate

E10992

0.5ml

E10994 E10991

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain, Lung Carcinoma

Control Tissue: Placenta

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Brain stained with anti-SMRTe antibody E1099

Human Placenta stained with anti-SOCS-6 antibody E1678

Product Description: SMRT is 1495 amino acids and contains an 8 amino acid sequence that is not present in SMRTe (SMRT-extended), which contains 2514 amino acids. SMRTe contains an N-terminal sequence spanning over 1,000 amino acids that is not present in SMRT, but that shows significant similarity with N-CoR. SMRTe expression is regulated during cell cycle progression, suggesting a role for SMRTe in the regulation of cyclespecific gene expression in diverse signaling pathways.

Product Description: Suppressor of Cytokine Signaling 6 (SOCS-6) interacts with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) overexpressing human IR. SOCS-1 and SOCS-6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. SOCS-6 proteins may be inhibitors of IR signaling and could mediate cytokine-induced insulin resistance and contribute to the pathogenesis of type II diabetes.

SOCS-2 Rabbit Polyclonal

Sodium Channel-pan Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E10290

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human SOCS-2 protein.

E10292

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E10294

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E10291

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16720

0.1ml

Concentrate

E16722

0.5ml

E16724 E16721

Clone: N/A Immunogen: Synthetic peptide from C-terminus of the alpha subunit of rat type 1 voltage-gated sodium channel. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas Cellular Localization: Cytoplasm

Control Tissue: Brain

Human Pancreas stained with anti-SOCS-2 antibody E1672

Human Brain stained with anti-Sodium Channel-pan antibody E1029

Cellular Localization: Cytoplasm

Product Description: Suppressor of Cytokine Signaling (SOCS) includes eight members of SOCS family (SOCS-1, 2, 3, 4, 5, 6, 7 and CIS). Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminus motif called the SOCS box. The SOCS-2 appear to form part of a classical negative feed back loop that regulates cytokine signal transduction.

Product Description: Voltage-gated sodium channels (VGSC) are present in most excitable cells. In neuronal tissue, they are responsible for generating and propagating action potentials. Brain VGSCs are heteromers of αβ1β2 subunits. Of these, the α subunit forms the channel pore. This antibody recognizes the alpha subunit of type I voltagegated sodium channel.

SOCS-3 Rabbit Polyclonal

Sonic Hedgehog Rabbit Polyclonal Clone: N/A

Catalog No.

Size Format

Clone: N/A

E17920

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human SOCS-3 protein.

E17922

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human sonic hedgehog.

1.0ml

Concentrate

Application: IHC-P

E17924

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E17921

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E16850

0.1ml

Concentrate

E16852

0.5ml

E16854 E16851

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta, Testis

Control Tissue: Fetal Brain, Lung

Cellular Localization: Cytoplasm

Cellular Localization: Extracellular Matrix, Membrane

Human Placenta stained with anti-SOCS-3 antibody E1685

Human Lung stained with anti-Sonic Hedgehog antibody E1792

Product Description: Suppressor of Cytokine Signaling 3 (SOCS-3) is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. It inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. It suppresses fetal liver erythropoiesis and regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells.

Product Description: Sonic Hedgehog (SHH) is expressed only during embryogenesis and is instrumental in patterning in the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

95


SOX-2 Rabbit Monoclonal Catalog No.

Size

Format

M3760

0.1ml

Concentrate

M3762

0.5ml

Concentrate

M3764

1.0ml

Concentrate

M3761

7.0ml

Ready-to-Use

SOX-9 Rabbit Polyclonal Clone: SP76

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to N-terminus of human SOX-2 protein.

E14040

0.1ml

Concentrate

E14042

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 316-510 of human SOX-9 protein.

E14044

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

E14041

7.0ml

Ready-to-Use

Species Reactivity: Human*, Mouse*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Testis

Control Tissue: Newborn Brain

Cellular Localization: Nucleus

Cellular Localization: Nucleus Control Lysate: MCF7 Molecular Weight: 34 kDa

Human Testis stained with anti-SOX-9 antibody E1404

Human Newborn Brain stained with anti-SOX-2 antibody M376

Product Description: SOX-2 is also known as SRY related HMG BOX gene 2. It is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency. SOX-2 is highly expressed in glioma, lung cancer and stomach cancer.

Product Description: SOX-9 is a member of the SOX family (Sry-type high mobility group box) genes that was first identified on the basis of region with high homology to that of SRY (Sex-determining region Y). SOX-9 is a transcription factor with a high mobility group DNA-binding domain in all prechondrocytic and chondrocytic cells during embryonic development. SOX-9 regulates expression of chondrocyte phenotype related genes.

SOX-2 Rabbit Polyclonal

SOX-9 Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

E13470

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human SOX-2 protein

E13472

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 1-150 of human SOX-9 protein.

1.0ml

Concentrate

Application: IHC-P

E13474

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E13471

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18600

0.1ml

Concentrate

E18602

0.5ml

E18604 E18601

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Newborn Brain, Skin

Control Tissue: Testis

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Newborn Brain stained with anti-SOX-2 antibody E1860

Human Testis stained with anti-SOX-9 antibody E1347

Product Description: SOX-2 is also known as SRY related HMG BOX gene 2. It is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency. SOX-2 is highly expressed in glioma, lung cancer and stomach cancer.

Product Description: SOX-9 is a member of the SOX family (Sry-type high mobility group box) genes that was first identified on the basis of region with high homology to that of SRY (Sex-determining region Y). SOX-9 is a transcription factor with a high mobility group DNA-binding domain in all prechondrocytic and chondrocytic cells during embryonic development. SOX-9 regulates expression of chondrocyte phenotype related genes.

SOX-4 Rabbit Polyclonal

SOX-11 Rabbit Polyclonal Catalog No.

Size

Format

E18760

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to internal region of human SOX-4 protein.

E18762

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human SOX-11 protein.

1.0ml

Concentrate

Application: IHC-P

E18764

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18761

7.0ml

Ready-to-Use

Size

Format

E16380

0.1ml

Concentrate

E16382

0.5ml

E16384 E16381

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Mantle Cell Lymphoma

Cellular Localization: Nucleus

Cellular Localization: Nucleus

Human Brain stained with anti-SOX-4 antibody E1638

Human Mantle Cell Lymphoma stained with anti-SOX-11 antibody E1876

Product Description: SOX-4 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. SOX-4 binds with high affinity to the T cell enhancer motif 5’-AACAAAG-3’ motif. Sox-4 is expressed in lymphocytes (B and T) and it is required for B lymphocyte development. It is also expressed in breast, breast cancer, medulloblastoma, and prostate cancer.

96

Clone: N/A

Clone: N/A

Catalog No.

Product Description: The SOX (SRY-related HMG-box) family are transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. All SOX proteins have a single HMG box and bind linear DNA. SOX-1, SOX-2 and SOX-3 show the closest homology to SRY. SOX-11 appears to be important in the development of nervous system and tissue modeling during development.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies SOX-14 Rabbit Polyclonal

Stanniocalcin-2 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E18840

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human SOX-14 protein.

E18842

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human stanniocalcin-2 protein.

1.0ml

Concentrate

Application: IHC-P

E18844

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E18841

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E16820

0.1ml

Concentrate

E16822

0.5ml

E16824 E16821

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Fetal Brain

Control Tissue: Small Intestine

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Fetal Brain stained with anti-SOX-14 antibody E1682

Human Small Intestine stained with anti-Stanniocalcin-2 antibody E1884

Product Description: The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNAbinding domain. They are involved in the regulation of embryonic development and in the determination of cell fate (specifically neuronal). SOX-14 is most closely related to SOX factors 1, 2 and 3 and likely mirrors expression of these genes in the developing brain and spinal cord.

Product Description: Stanniocalcin-2 is a homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport and cell metabolism. In the ovary, it has been shown to be a paracrine hormone that regulates granulosa cell function. Expression of this gene is induced by estrogen and altered in some breast cancers.

SP1 Rabbit Polyclonal

STAT3 Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

E3690

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human SP1.

E3692

0.5ml

Concentrate

Immunogen: Recombinant protein encoding aa 50-240 of human Stat3.

1.0ml

Concentrate

Application: IHC-P

E3694

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3691

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E12540

0.1ml

Concentrate

E12542

0.5ml

E12544 E12541

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Breast Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Nucleus

Human Tonsil stained with anti-SP1 antibody E1254

Human Breast Carcinoma stained with anti-STAT3 antibody E369

Product Description: SP1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. SP1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences.

Product Description: The STAT3 protein is a transcription factor expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, IL-12, IL-2, EGF etc. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE (acute phase response element) and SIE (c-sis inducible element).

ST13 Rabbit Polyclonal

STAT5 alpha Rabbit Polyclonal Clone: N/A

Catalog No.

Size Format

Clone: N/A

E3590

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to human ST13.

E3592

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human STAT5 alpha.

1.0ml

Concentrate

Application: IHC-P

E3594

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3591

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E17870

0.1ml

Concentrate

E17872

0.5ml

E17874 E17871

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Breast Carcinoma, Tonsil

Cellular Localization: Nucleus

Cellular Localization: Nucleus Control Lysate: K562 Molecular Weight: 95 kDa Human Breast Carcinoma stained with anti-STAT5 alpha antibody E359

Human Colon Carcinoma stained with anti-ST13 antibody E1787

Product Description: ST13 is a cofactor of heat shock protein 70 (HSP70). To date, all data since the discovery of ST13 in 1993 until more recent studies in 2007 have proved that ST13 is down regulated in tumors and it is proposed to be a tumor suppressor gene.

Product Description: STAT5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct STAT5 genes (STAT5a and STAT5b) were identified in mouse. STAT5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

97


STAT6 Rabbit Polyclonal

Survivin Rabbit Monoclonal Clone: N/A

Catalog No.

Size

Format

M3790

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human STAT6.

M3792

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

M3794 M3791

1.0ml 7.0ml

Concentrate Ready-to-Use

Catalog No.

Size

Format

E4290

0.1ml

Concentrate

E4292

0.5ml

E4294 E4291

Species Reactivity: Human*

Clone: SP79 Immunogen: Synthetic peptide corresponding to C-terminus of human survivin protein. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta, Tonsil

Control Tissue: Stomach

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm

Human Placenta stained with anti-STAT6 antibody E429

Human Stomach stained with anti-Survivin antibody M379

Product Description: STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. STAT6 was found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, and T-cell proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response.

Product Description: Survivin is an inhibitor of apoptosis (IAP) protein. It is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

Stathmin Rabbit Monoclonal

Survivin Rabbit Polyclonal Clone: SP49

Catalog No.

Size

Format

E3770

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human stathmin.

E3772

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human survivin.

1.0ml

Concentrate

Application: IHC-P, WB

E3774

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*, Mouse*

E3771

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3490

0.1ml

Concentrate

M3492

0.5ml

M3494 M3491

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Stomach

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Control Lysate: NIH3T3 Molecular Weight: 18 kDa Human Stomach stained with anti-Survivin antibody E377

Human Tonsil stained with anti-Stathmin antibody M349

Product Description: Stathmin is a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Alternatively spliced transcript variants encoding the same protein have been identified.

Product Description: Survivin is an inhibitor of apoptosis (IAP) protein. It is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

Stathmin Rabbit Polyclonal

SYK Rabbit Polyclonal Catalog No.

Size

Format

E4050

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human stathmin.

E4052

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from internal region of human SYK protein.

1.0ml

Concentrate

Application: IHC-P, WB

E4054

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4051

7.0ml

Ready-to-Use

Size

Format

E18060

0.1ml

Concentrate

E18062

0.5ml

E18064 E18061

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma, Tonsil

Control Tissue: Bone Marrow, Lymphoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Control Lysate: NIH3T3

Control Lysate: HepG2

Molecular Weight: 18 KDa

Molecular Weight: 72 KDa

Human Breast Carcinoma stained with anti-Stathmin antibody E1806

Human Bone Marrow stained with anti-SYK antibody E405

Product Description: Stathmin is a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Alternatively spliced transcript variants encoding the same protein have been identified.

98

Clone: N/A

Clone: N/A

Catalog No.

Product Description: SYK, a protein tyrosine kinase (PTK) is expressed in immature T cells, all B cells, basophils, hepatocytes, platelets, and erythrocytes. It functions in some T cell receptor signaling processes in addition to B cell receptor and integrin-mediated signal transduction processes. SYM-/syk- mice exhibit defective phagocytosis by macrophages.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Synaptophysin Rabbit Monoclonal Catalog No.

Size

Format

M3110

0.1ml

Concentrate

M3112

0.5ml

Concentrate

M3114

1.0ml

Concentrate

M3111

7.0ml

Ready-to-Use

Syntaxin 1A Rabbit Polyclonal

Clone: SP11

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to C-terminus of human synaptophysin.

E17700

0.1ml

Concentrate

E17702

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human syntaxin 1A protein.

E17704

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

E17701

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Breast Neuroendocrine Tumor, Pancreas, Pheochromcytoma

Cellular Localization: Membrane

Cellular Localization: Cytoplasm Control Lysate: Human Brain Molecular Weight: 38 kDa

Human Brain stained with anti-Syntaxin 1A antibody E1770

Human Neuroendocrine Tumor stained with anti-Synaptophysin antibody M311

Product Description: Synaptophysin is expressed in neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells, neurons in the brain, spinal cord, and retina. It is also expressed in neuroendocrine tumors such as neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non-chromaffin paragangliomas.

Product Description: Syntaxin 1A (STX1A) is potentially involved in docking of synaptic vesicles at presynaptic active zones and may play a critical role in neurotransmitter exocytosis. Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculoskeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.

Synaptophysin Rabbit Polyclonal

Synuclein-alpha Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E2680

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide of human synaptophysin.

E2682

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to near C-terminus of human synuclein-alpha.

1.0ml

Concentrate

Application: IHC-P

E2684

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E2681

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E2170

0.1ml

Concentrate

E2172

0.5ml

E2174 E2171

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon, Pancreas

Control Tissue: Brain (Posterior Cingulated Gyrus)

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Presynaptic Terminals

Human Pancreas stained with anti-Synaptophysin antibody E217

Human Brain stained with anti-Synuclein-alpha antibody E268

Product Description: Synaptophysin is expressed in neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells, neurons in the brain, spinal cord, and retina. It is also expressed in neuroendocrine tumors such as neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non-chromaffin paragangliomas.

Product Description: Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha-synuclein, beta-synuclein, and gamma-synuclein. The alpha- and beta-synuclein proteins are found primarily in brain tissue, where they are seen mainly in presynaptic terminals. This antibody is specific for alpha-synuclein and is useful for the localization and detection of alpha-synuclein.

Syntaxin 1A Rabbit Monoclonal

Synuclein-beta Rabbit Polyclonal

Clone: SP36

Catalog No.

Size Format

Clone: N/A

E2770

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human syntaxin 1A protein.

E2772

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from near C-terminus of human synuclein-beta.

1.0ml

Concentrate

Application: IHC-P

E2774

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*, Mouse*

E2771

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

M3360

0.1ml

Concentrate

M3362

0.5ml

M3364 M3361

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain

Control Tissue: Brain (Posterior Cingulated Gyrus)

Cellular Localization: Membrane

Cellular Localization: Cytoplasm, Presynaptic Terminals

Human Brain stained with anti-Syntaxin 1A antibody M336

Human Brain stained with anti-Synuclein-beta antibody E277

Product Description: Syntaxin 1A (STX1A) is potentially involved in docking of synaptic vesicles at presynaptic active zones and may play a critical role in neurotransmitter exocytosis. Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculoskeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.

Product Description: Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha-synuclein, beta-synuclein, and gamma-synuclein. The alpha- and beta-synuclein proteins are found primarily in brain tissue, where they are seen mainly in presynaptic terminals. This antibody is specific for beta-synuclein and does not cross react with alpha-and gammasynuclein.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

99


Synuclein-pan Rabbit Polyclonal

TdT Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E19230

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from near N-terminus of human α, β, γ-Synuclein protein.

E19232

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

E19234 E19231

1.0ml 7.0ml

Concentrate Ready-to-Use

Catalog No.

Size

Format

E2790

0.1ml

Concentrate

E2792

0.5ml

E2794 E2791

Species Reactivity: Human*

Clone: N/A Immunogen: Synthetic peptide in the internal region of human TdT protein. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Brain (Posterior Cingulated Gyrus)

Control Tissue: Thymus, Thymomas

Cellular Localization: Cytoplasm, Presynaptic Terminals

Cellular Localization: Nucleus

Human Brain stained with anti-Synuclein-pan antibody E279

Human Thymus stained with anti-TdT antibody E1923

Product Description: Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha-synuclein, beta-synuclein, and gamma-synuclein. The alpha- and beta- synuclein proteins are found primarily in brain tissue. This antibody detects all synuclein proteins.

Product Description: Terminal deoxynucleotidyl transferase (TdT) is a nuclear DNA polymerase responsible for the template-independent addition of N-nucleotides. It is expressed in normal hematopoietic cells, normal cortical thymocytes (immature, pre-B, pre-T lymphoid cells), and megakaryocytes of the bone marrow as well as acute lymphoblastic leukemia/lymphoma cells.

Tau (Neuronal Marker) Rabbit Monoclonal

Thioredoxin Reductase 2 (TrxR2) Rabbit Polyclonal

Clone: SP70

Catalog No.

Size

Format

E3610

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human tau protein.

E3612

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human TrxR2.

1.0ml

Concentrate

Application: IHC-P

E3614

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3611

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M3700

0.1ml

Concentrate

M3702

0.5ml

M3704 M3701

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Liver Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Breast Carcinoma stained with anti-Tau (Neuronal Marker) antibody M370

Human Liver Carcinoma stained with anti-Thioredoxin Reductase 2 (TrxR2) antibody E361

Product Description: Tau is a neuronal microtubule associated protein found mainly on axons. Its function is to promote tubulin polymerization and stabilize microtubules and to link certain signaling pathways to the cytoskeleton. Tau is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destruction of microtubules and neurons in AD brain.

Product Description: The reduced form of thioredoxin serves as a hydrogen donor, contributes to the up-regulation of various transcription factors and binds to and inhibits MAPKKK activity. Oxidized thioredoxin is converted back to the reduced form by Thioredoxin Reductase (TrxR2), a redox active enzyme. There are two distinct isoforms in mammalian cells, TrxR1, a cytosolic protein and TrxR2, almost exclusively a mitochondrial protein. TrxR expression is induced in several tumors.

Tau (Neuronal Marker) Rabbit Polyclonal

Thrombin R/ATAP2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E12530

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to N-terminus of human tau protein.

E12532

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E12534

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E12531

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18290

0.1ml

Concentrate

E18292

0.5ml

E18294 E18291

Isotype: Rabbit IgG Control Tissue: Alzheimer’s Brain, Breast Carcinoma Cellular Localization: Cytoplasm

Clone: N/A Immunogen: Synthetic peptide corresponding to near N-terminus of human thrombin R. Application: IHC-P, WB Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Pancreas Cellular Localization: Cytoplasm, Membrane Control Lysate: HeLa Molecular Weight: 66 kDa

Human Breast Carcinoma stained with anti-Tau (Neuronal Marker) antibody E1829

Product Description: Tau is a neuronal microtubule associated protein found mainly on axons. Its function is to promote tubulin polymerization and stabilize microtubules and to link certain signaling pathways to the cytoskeleton. Tau is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destruction of microtubules and neurons in AD brain.

100

Human Pancreas stained with anti-Thrombin R/ATAP2 antibody E1253

Product Description: Thrombin is a serine protease that is involved in platelet aggregation and blood coagulation. It is cleaved from its precursor, prothrombin, and converts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediates its regulatory effects by activating cell surface G-protein coupled receptors which include PAR-1, PAR-2 and PAR3.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Thymidylate Synthase (TS) Rabbit Monoclonal

Thyroglobulin Mouse Monoclonal

Clone: SP112

Catalog No.

Size

Format

E16600

0.1ml

Concentrate

Immunogen: Human thyroglobulin.

Concentrate

Immunogen: Synthetic peptide near C-terminus of human TS.

E16602

0.5ml

Concentrate

Concentrate

Application: IHC-P

Application: IHC-P

1.0ml

E16604

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E16601

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

M4120

0.1ml

Concentrate

M4122

0.5ml

M4124 M4121

Isotype: Rabbit IgG

Clone: SPM517

Isotype: Mouse IgG1 Control Tissue: Thyroid

Control Tissue: Colon Carcinoma, Tonsil

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Nucleus

Human Colon Carcinoma stained with anti-Thymidylate Synthase (TS) antibody M412

Human Thyroid stained with anti-Thyroglobulin antibody E1660

Product Description: Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma.

Product Description: Thyroglobulin is expressed in thyroid carcinomas of the papillary and follicular types. Adenocarcinomas of non-thyroid origin are not reactive.

Thymidylate Synthase (TS) Rabbit Polyclonal

Thyroid Hormone Receptor alpha1/alpha2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E4060

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human TS protein.

E4062

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

E4064 E4061

1.0ml 7.0ml

Concentrate Ready-to-Use

Catalog No.

Size

Format

E18570

0.1ml

Concentrate

E18572

0.5ml

E18574 E18571

Species Reactivity: Human*

Clone: N/A Immunogen: Synthetic peptide derived from N-terminus of human TR-alpha 1 protein. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Thyroid

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Nucleus

Human Colon Carcinoma stained with anti-Thymidylate Synthase (TS) antibody E1857

Human Thyroid stained with anti-Thyroid Hormone Receptor alpha1/alpha2 antibody E406

Product Description: Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) during DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS. Over expression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma.

Product Description: Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TRalpha and TRbeta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes.

Thymidylate Synthase (TS) Rabbit Polyclonal

Thyroid Hormone Receptor beta Rabbit Polyclonal

Clone: N/A

Catalog No.

Size Format

Clone: N/A

E4070

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to human TS.

E4072

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human TR-beta protein.

1.0ml

Concentrate

Application: IHC-P

E4074

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4071

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E17910

0.1ml

Concentrate

E17912

0.5ml

E17914 E17911

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Thyroid

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Nucleus

Human Colon Carcinoma stained with anti-Thymidylate Synthase (TS) antibody E1791

Human Thyroid stained with anti-Thyroid Hormone Receptor beta antibody E407

Product Description: Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) during DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS. Over expression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma.

Product Description: Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TRalpha and TRbeta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

101


Thyroid Stimulating Hormone (TSH) Rabbit Polyclonal

TIMP-2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Purified human TSH.

E4180

0.1ml

Concentrate

Concentrate

Application: IHC-P

E4182

0.5ml

Concentrate

1.0ml

Concentrate

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4184 E4181

1.0ml 7.0ml

Concentrate Ready-to-Use

Catalog No.

Size

Format

E17500

0.1ml

E17502

0.5ml

E17504 E17501

Isotype: Rabbit IgG

Clone: N/A Immunogen: Synthetic peptide derived from N-terminus half of human TIMP-2. Application: IHC-P Species Reactivity: Human* Isotype: Rabbit IgG

Control Tissue: Anterior Pituitary Cellular Localization: Cytoplasm

Control Tissue: Colon Carcinoma

Human Anterior Pituitary stained with anti-Thyroid Stimulating Hormone (TSH) antibody E1750

Human Colon Carcinoma stained with anti-TIMP-2 antibody E418

Cellular Localization: Cytoplasm

Product Description: TSH is a pituitary hormone of 28kDa, which stimulates thyroid growth and production of thyroid hormones. This antibody labels thyrotropic cells of the pituitary.

Product Description: Tissue inhibitor of metalloproteinases-1 and 2 (TIMP-1 and TIMP-2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex with activated interstitial collagenases, stromelysin, and type IV collagenase. TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

TIE-1 Rabbit Polyclonal

TIMP-3 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E3850

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human TIE-1.

E3852

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human TIMP-3.

1.0ml

Concentrate

Application: IHC-P

E3854

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3851

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E17520

0.1ml

Concentrate

E17522

0.5ml

E17524 E17521

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Breast Carcinoma, Placenta

Cellular Localization: Membrane

Cellular Localization: Cytoplasm

Human Tonsil stained with anti-TIE-1 antibody E1752

Human Placenta stained with anti-TIMP-3 antibody E385

Product Description: TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek are expressed primarily on endothelial and hematopoietic progenitor cells, and play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. The in vivo functions of TIE1 are related to endothelial cell differentiation.

Product Description: TIMPs (Tissue Inhibitors of Metallo Proteinases) form tight inhibitory complexes with MMPs which are involved in matrix degradation and hence, in tumor invasiveness and metastasis. TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

TIMP-1 Rabbit Polyclonal

TNF-R2 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E4320

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from N-terminus half of human TIMP-1.

E4322

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of mouse TNF-R2.

1.0ml

Concentrate

Application: IHC-P

E4324

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4321

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3360

0.1ml

Concentrate

E3362

0.5ml

E3364 E3361

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Bladder, Breast, Colon Carcinoma, Ovarian Carcinoma

Control Tissue: Liver Carcinoma, Thymus

Cellular Localization: Cytoplasm

Human Colon Carcinoma stained with anti-TIMP-1 antibody E336

Product Description: Tissue inhibitor of metalloproteinases-1 and 2 (TIMP-1 and TIMP-2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex with activated interstitial collagenases, stromelysin, and type IV collagenase. TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

102

Clone: N/A

Cellular Localization: Cytoplasm

Human Liver Carcinoma stained with anti-TNF-R2 antibody E432

Product Description: Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2, are 55kDa and 75kDa respectively, and are expressed on most cell types. Signaling through TNF-R2 is confined to cells of the immune system.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Topo II beta Rabbit Polyclonal

TRADD Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E4340

0.1ml

Concentrate

Concentrate

Immunogen: Recombinant of human topoisomerase II beta protein.

E4342

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human TRADD.

1.0ml

Concentrate

Application: IHC-P

E4344

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4341

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E4140

0.1ml

Concentrate

E4142

0.5ml

E4144 E4141

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Tonsil

Control Tissue: Liver Carcinoma

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm

Human Tonsil stained with anti-Topo II beta antibody E414

Human Liver Carcinoma stained with anti-TRADD antibody E434

Product Description: Topo II beta plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells.

Product Description: TRADD (TNF-R1-associated death domain) is an important mediator of FAS and TNF-r1 associated apoptotic signaling. It contains a death domain homologous region (DDH) through which it binds to both FAS and TNF-R1. Overexpression of TRADD causes NFkB activation and apoptosis in absence of TNF.

Topoisomerase I Rabbit Polyclonal

Transforming Growth Factor beta R3 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size Format

Clone: N/A

E11230

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human topoisomerase I protein.

E11232

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

E11234

1.0ml

Concentrate

Immunogen: Recombinant human TGF beta Type III receptor protein encoding aa 644-850 expressed in E. coli.

7.0ml

Ready-to-Use

Species Reactivity: Human*

E11231

7.0ml

Ready-to-Use

Application: IHC-P

Catalog No.

Size

Format

E18860

0.1ml

Concentrate

E18862

0.5ml

E18864 E18861

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Placenta

Cellular Localization: Cytoplasm, Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Placenta stained with anti-Transforming Growth Factor beta R3 antibody E1123

Human Breast Carcinoma stained with anti-Topoisomerase I antibody E1886

Product Description: Topoisomerases are nuclear enzymes involved in chromosome condensation, DNA replication, transcription, recombination and segregation during mitosis. Human topoisomerase I is a 100kDa protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick. Autoantibodies for both DNA topoisomerases I and II were found to in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus.

Product Description: A total of three members of the TGF beta family, TGF beta1, TGF beta2 and TGF beta3, have been identified in mammals. The type III TGF beta receptor, also referred to as beta-glycan, confers an additional level of regulation to the TGF beta signaling system by influencing the affinity with which the TGF betas bind to their respective receptors.

Toxoplasma Gondii Rabbit Polyclonal

Transforming Growth Factor beta1 (TGFb1) Rabbit Polyclonal

Catalog No.

Size

Format

Clone: N/A

Catalog No.

Size Format

Clone: N/A

E4520

0.1ml

Concentrate

Immunogen: Toxoplasma gondii.

E11260

0.1ml

Concentrate

E4522

0.5ml

Concentrate

Application: IHC-P

E11262

0.5ml

Concentrate

Immunogen: Recombinant protein located in aa 177-391 of human TGF beta 1.

E4524

1.0ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E11264

E4521

E11261

7.0ml

Ready-to-Use

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Toxoplasma Gondii Infected Tissue

Control Tissue: Placenta

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Placenta stained with anti-Transforming Growth Factor beta1 (TGFb1) antibody E1126

Human Toxoplasma Gondii Infected Brain stained with anti-Toxoplasma Gondii antibody E452

Product Description: Toxoplasma is as an intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/ undercooked meat, contaminated soil, or by direct contact. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild. However congenital toxoplasmosis can produce blindness or mental retardation in the newborn.

Product Description: A total of three members of the TGF beta family, TGF beta1, TGF beta2 and TGF beta3, have been identified in mammals. TGF betas mediate their activity by high affinity binding to the type II receptor transmembrane protein with a cytoplasmic serine/threonine kinase domain.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

103


Transforming Growth Factor beta2 (TGFb2) Rabbit Polyclonal

Transglutaminase II Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

E4170

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from internal region of human TGF beta 2.

E4172

0.5ml

Concentrate

Immunogen: Recombinant full length human transglutaminase II protein.

1.0ml

Concentrate

Application: IHC-P

E4174

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4171

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E18020

0.1ml

Concentrate

E18022

0.5ml

E18024 E18021

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Extracellular Matrix, Membrane

Human Placenta stained with anti-Transforming Growth Factor beta2 (TGFb2) antibody E1802

Human Breast Carcinoma stained with anti-Transglutaminase II antibody E417

Product Description: Transforming growth factor betas (TGFbs) mediate many cell-cell interactions that occur during embryonic development. Three TGFbs (TGFb1, TGFb2 and TGFb3) have been identified in mammals. They are synthesized as precursor proteins and then cleaved to yield a 112 amino acid polypeptide. This antibody reacts with the precursor and mature form of TGFb2 and to a lesser extent TGF3, but does not cross react with TGFb1.

Product Description: TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Transforming Growth Factor beta3 (TGFb3) Rabbit Polyclonal

TTF-1 Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

E19180

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of the precursor form of human TGF beta 3.

E19182

0.5ml

Concentrate

Immunogen: Synthetic peptide in the internal region of human TTF-1 protein.

1.0ml

Concentrate

Application: IHC-P

E19184

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E19181

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E3940

0.1ml

Concentrate

E3942

0.5ml

E3944 E3941

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Placenta

Control Tissue: Lung, Lung Adenocarcinoma, Thyroid

Cellular Localization: Cytoplasm

Cellular Localization: Nucleus Control Lysate: HeLa Molecular Weight: 39 kDa

Human Placenta stained with anti-Transforming Growth Factor beta3 (TGFb3) antibody E394

Human Thyroid stained with anti-TTF-1 antibody E1918

Product Description: A total of three members of the TGF beta family, TGF beta1, TGF beta2 and TGF beta3, have been identified in mammals. The type III TGF beta receptor, also referred to as beta-glycan, confers an additional level of regulation to the TGF beta signaling system by influencing the affinity with which the TGF betas bind to their respective receptors.

Product Description: Thyroid transcription factor-1 (TTF-1) is a member of the NKx2 family of homeodomain transcription factors. It binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. TTF-1 is important in the maintenance of thyroid differentiation and lung development. It is expressed in epithelial cells of the thyroid gland and the lung, and tumors derived from such epithelial cells.

Transforming Growth Factor R1 Rabbit Polyclonal

TTF-1 Mouse Monoclonal

Catalog No.

Size

Format

E12340

0.1ml

Concentrate

E12342

0.5ml

Concentrate

E12344 E12341

1.0ml 7.0ml

Concentrate Ready-to-Use

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to internal region of human TGF beta R1.

E17080

0.1ml

Concentrate

Immunogen: Rat TTF-1 recombinant protein.

E17082

0.5ml

Concentrate

Application: IHC-P

Application: IHC-P

E17084

1.0ml

Concentrate

Species Reactivity: Human*

E17081

7.0ml

Ready-to-Use

Species Reactivity: Human*

Isotype: Rabbit IgG Control Tissue: Placenta Cellular Localization: Membrane

Human Placenta stained with anti-Transforming Growth Factor R1 antibody E1234

Product Description: A total of three members of the TGF beta family, TGF beta1, TGF beta2 and TGF beta3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGFbetas mediate their activity by high affinity binding to the type II receptor 70kDa transmembrane protein with a cytoplasmic serine/ threonine kinase domain.

104

Clone: 8G7G3/1

Isotype: Mouse IgG1/k Control Tissue: Lung, Thyroid Cellular Localization: Nucleus

Human Lung stained with anti-TTF-1 antibody E1708

Product Description: TTF-1 (Thyroid transcription factor-1) is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. TTF-1 is detected in primary lung adenocarcinomas and small cell carcinomas and is absent in colon and breast carcinomas.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Tubulin-alpha Rabbit Polyclonal

Ubiquitin Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

E3350

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human tubulin alpha.

E3352

0.5ml

Concentrate

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

E3354 E3351

1.0ml 7.0ml

Concentrate Ready-to-Use

Catalog No.

Size

Format

E4130

0.1ml

Concentrate

E4132

0.5ml

E4134 E4131

Species Reactivity: Human*

Clone: N/A Immunogen: Recombinant full length human ubiquitin protein. Application: IHC-P Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung

Control Tissue: Alzheimer’s Brain, Pancreas

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane, Nucleus

Human Lung stained with anti-Tubulin-alpha antibody E413

Human Alzheimer’s Brain stained with anti-Ubiquitin antibody E335

Product Description: Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of tubulin, which is a dimer of two 55kDa subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

Product Description: Ubiquitin (Ub) plays a very important role in regulated nonlysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

Tubulin-beta Rabbit Polyclonal

Urocortin Rabbit Polyclonal

Clone: N/A

Catalog No.

Size Format

Clone: N/A

E4480

0.1ml

Concentrate

E4482

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of urocortin.

Catalog No.

Size

Format

E3810

0.1ml

Concentrate

E3812

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human tubulin beta.

E3814

1.0ml

Concentrate

Application: IHC-P

E4484

1.0ml

Concentrate

Application: IHC-P

E3811

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4481

7.0ml

Ready-to-Use

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Lung, Skin

Control Tissue: Pituitary

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm

Human Lung stained with anti-Tubulin-beta antibody E381

Human Pituitary stained with anti-Urocortin antibody E448

Product Description: Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of tubulin, which is a dimer of two 55kDa subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. This antibody blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.

Product Description: Urocortin (UCN), a neuropeptide widely distributed in the central nervous system (CNS), belongs to a family of structurally related peptides including corticotrophin releasing factor (CRF), sauvagine and urotensin I. Urocortin is derived from a larger precursor protein (pro-urocortin). Urocortin has biological actions similar to CRF. It is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary.

Tubulin-gamma Rabbit Polyclonal

Uroplakin III Rabbit Monoclonal

Clone: N/A

Catalog No.

Size Format

Clone: SP73

M3730

0.1ml

Concentrate

M3732

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human uroplakin III protein.

Catalog No.

Size

Format

E10330

0.1ml

Concentrate

E10332

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human tubulin gamma protein.

E10334

1.0ml

Concentrate

Application: IHC-P

M3734

1.0ml

Concentrate

Application: IHC-P

E10331

7.0ml

Ready-to-Use

Species Reactivity: Human*

M3731

7.0ml

Ready-to-Use

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Colon Carcinoma

Control Tissue: Bladder, Bladder Carcinoma

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Colon Carcinoma stained with anti-Tubulin-gamma antibody E1033

Human Bladder Carcinoma stained with anti-Uroplakin III antibody M373

Product Description: Tubulin gamma, an essential structural protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm. Tubulin gamma-depleted cells fail to form functional spindles and arrest during nuclear division. Tubulin gamma associates with spindle body components Spc97 and Spc98 to form the tubulin gamma complex. The phosphorylation of tubulin gamma at Tyr445 plays a regulatory role in microtubule formation.

Product Description: Uroplakins are specific differentiation products of terminally differentiated superficial urothelial cells. Uroplakin III is expressed in primary and metastatic urothelial carcinomas.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

105


Uroplakin III Rabbit Polyclonal Catalog No.

Size

Format

E18240

0.1ml

Concentrate

E18242

0.5ml

Concentrate

E18244

1.0ml

Concentrate

E18241

7.0ml

Ready-to-Use

Vascular Endothelial Growth Factor (VEGF) Rabbit Polyclonal

Clone: N/A

Catalog No.

Size

Format

Immunogen: Synthetic peptide corresponding to C-terminus of human uroplakin III.

E2610

0.1ml

Concentrate

E2612

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human VEGF.

E2614

1.0ml

Concentrate

Application: IHC-P, WB

Application: IHC-P

E2611

7.0ml

Ready-to-Use

Species Reactivity: Human*

Clone: N/A

Species Reactivity: Human* Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Angiosarcoma

Control Tissue: Bladder, Bladder Carcinoma

Cellular Localization: Cytoplasm, Extracellular Matrix, Membrane

Cellular Localization: Cytoplasm, Membrane Control Lysate: Human Bladder Molecular Weight: 32 kDa

Human Angiosarcoma stained with anti-Vascular Endothelial Growth Factor (VEGF) antibody E261

Human Bladder Carcinoma stained with anti-Uroplakin III antibody E1824

Product Description: Uroplakins are specific differentiation products of terminally differentiated superficial urothelial cells. Uroplakin III is expressed in primary and metastatic urothelial carcinomas.

Product Description: VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. This antibody reacts with the 165, 189 and 121 amino acid splice variants of human VEGF.

USF-2 Rabbit Polyclonal

Villin Rabbit Polyclonal Clone: N/A

Clone: N/A

Catalog No.

Size

Format

Concentrate

Immunogen: Synthetic peptide corresponding to aa 327-346 of mouse USF-2.

E16640 E16642

0.1ml 0.5ml

Concentrate Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human villin.

1.0ml

Concentrate

Application: IHC-P

E16644

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E16641

7.0ml

Ready-to-Use

Catalog No.

Size

Format

E12560

0.1ml

Concentrate

E12562

0.5ml

E12564 E12561

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Breast Carcinoma

Control Tissue: Small Intestine

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Membrane

Human Breast Carcinoma stained with anti-USF-2 antibody E1256

Human Small Intestine stained with anti-Villin antibody E1664

Product Description: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of 43kDa (USF-1) and 44kDa (USF-2) polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding.

Product Description: Villin is an actin binding protein expressed mainly in the brush border of the epithelium in vertebrates. Villin can cap, nucleate, sever and bundle actin in a Ca2+ and phosphoinositide-regulated manner. Villin is composed of six repeats that together constitute the core domain. The core domain retains the Ca2+ dependent capping nucleating and severing activity, whereas the headpiece domain contributes towards actin filament bundling and binding F-actin.

Vascular Endothelial Growth Factor (VEGF) Rabbit Monoclonal

Vimentin Rabbit Monoclonal

Catalog No.

Size

Format

N/A

N/A

N/A

N/A

N/A

N/A

N/A M3281

N/A 7.0ml

N/A Ready-to-Use

Clone: SP28

Catalog No.

Size

Format

Immunogen: Synthetic peptide derived from N-terminus of human VEGF.

M3200

0.1ml

Concentrate

Clone: SP20

M3202

0.5ml

Concentrate

Immunogen: Recombinant human vimentin protein.

Application: IHC-P

M3204

1.0ml

Concentrate

Application: IHC-P, WB

Species Reactivity: Human*

M3201

7.0ml

Ready-to-Use

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Angiosarcoma

Control Tissue: Melanoma, Sarcomas

Cellular Localization: Cytoplasm, Extracellular Matrix, Membrane

Cellular Localization: Cytoplasm Control Lysate: HeLa Molecular Weight: 53 kDa

Human Angiosarcoma stained with anti-Vascular Endothelial Growth Factor (VEGF) antibody M328

Product Description: VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. This antibody reacts with the 165, 189 and 121 amino acid splice variants of human VEGF.

106

Human Melanoma stained with anti-Vimentin antibody M320

Product Description: Vimentin is the main intermediate filament protein in mesenchymal cells. This protein is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is expressed in sarcomas of neural, muscle and fibroblast origin, but carcinomas are generally negative. Melanomas, lymphomas and vascular tumors may express vimentin.

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


Primary Antibodies Vinculin Rabbit Monoclonal

WNT-1 Rabbit Polyclonal Clone: SP117

Catalog No.

Size

Format

E3960

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human vinculin protein.

E3962

0.5ml

Concentrate

Immunogen: Synthetic peptide derived from N-terminus of human WNT-1.

1.0ml

Concentrate

Application: IHC-P

E3964

1.0ml

Concentrate

Application: IHC-P, WB

7.0ml

Ready-to-Use

Species Reactivity: Human*

E3961

7.0ml

Ready-to-Use

Catalog No.

Size

Format

M4170

0.1ml

Concentrate

M4172

0.5ml

M4174 M4171

Clone: N/A

Species Reactivity: Human*

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Heart, Small Intestine

Control Tissue: Breast Carcinoma

Cellular Localization: Cytoplasm, Membrane

Cellular Localization: Cytoplasm Control Lysate: NIH3T3 Molecular Weight: 42 kDa Human Breast Carcinoma stained with anti-WNT-1 antibody E396

Human Heart stained with anti-Vinculin antibody M417

Product Description: Vinculin is a muscle-specific cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane and regulating cell-surface E-cadherin expression. The defect of this protein can cause dilated cardiomyopathy

Product Description: Wnt-1 is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids.

Vinculin Rabbit Polyclonal

XMRV Rabbit Polyclonal Clone: N/A

Catalog No.

Size Format

Clone: N/A

E19070

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide derived from C-terminus of human vinculin protein.

E19072

0.5ml

Concentrate

Immunogen: Synthetic peptide near N-terminus of XMRV envelope protein.

1.0ml

Concentrate

Application: IHC-P

E19074

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E19071

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E18720

0.1ml

Concentrate

E18722

0.5ml

E18724 E18721

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Pancreas, Small Intestine

Control Tissue: Prostate Carcinoma with XMRV Infection

Cellular Localization: Cytoplasm

Cellular Localization: Cytoplasm, Membrane

Human Pancreas stained with anti-Vinculin antibody E1872

Human Prostate Carcinoma with XMRV Infection stained with anti-XMRV antibody E1907

Product Description: Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. Vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Product Description: Xenotropic murine leukemia virus-related virus (XMRV) is a newly recognized human retrovirus first identified in prostate cancer tissues from men with a deficiency in the innate immunity gene RNASEL. At present, studies have detected XMRV DNA and protein in prostate cancer at widely different rates and in patients with chronic fatigue syndrome. The XMRV protein is synthesized in the Golgi by host cells.

Wilm’s Tumor (WT) Rabbit Polyclonal

XRCC2 Rabbit Polyclonal

Clone: N/A

Catalog No.

Size Format

Clone: N/A

E4010

0.1ml

Concentrate

Concentrate

Immunogen: Synthetic peptide from C-terminus of human Wilm’s tumor protein.

E4012

0.5ml

Concentrate

Immunogen: Synthetic peptide from N-terminus of human XRCC2.

1.0ml

Concentrate

Application: IHC-P

E4014

1.0ml

Concentrate

Application: IHC-P

7.0ml

Ready-to-Use

Species Reactivity: Human*

E4011

7.0ml

Ready-to-Use

Species Reactivity: Human*

Catalog No.

Size

Format

E3990

0.1ml

Concentrate

E3992

0.5ml

E3994 E3991

Isotype: Rabbit IgG

Isotype: Rabbit IgG

Control Tissue: Wilm’s Tumor

Control Tissue: Testis

Cellular Localization: Nucleus

Cellular Localization: Cytoplasm, Nucleus

Human Wilm’s Tumor stained with anti-Wilm’s Tumor (WT) antibody E399

Human Testis stained with anti-XRCC2 antibody E401

Product Description: Wilm’s tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilm’s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelioma.

Product Description: XRCC2 (X-Ray Cross Complementing 2) is essential for repairing the double strand DNA breaks by homologous recombination between sister chromatids. It is homologous to Rad51. Five human homologues of RAD 51; XRCC2, XRCC3, RAD51B, RAD51C, RAD51D, interact among themselves to form one big complex or several smaller complexes.

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

107


ZAP-70 Rabbit Polyclonal Catalog No.

Size

Format

E12520

0.1ml

Concentrate

E12522

0.5ml

Concentrate

E12524

1.0ml

Concentrate

E12521

7.0ml

Ready-to-Use

Clone: N/A Immunogen: Synthetic peptide corresponding to C-terminus of human ZAP-70. Application: IHC-P, WB Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Tonsil Cellular Localization: Cytoplasm Control Lysate: Jurkat Molecular Weight: 70 kDa Human Tonsil stained with anti-ZAP-70 antibody E1252

Product Description: Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 is also expressed in a subset of Chronic Lymphocytic Leukemia (CLL).

ZO-3 Rabbit Polyclonal Clone: N/A

Catalog No.

Size

Format

E17450

0.1ml

Concentrate

E17452

0.5ml

Concentrate

Immunogen: Synthetic peptide corresponding to C-terminus of human ZO-3.

E17454

1.0ml

Concentrate

Application: IHC-P

E17451

7.0ml

Ready-to-Use

Species Reactivity: Human* Isotype: Rabbit IgG Control Tissue: Small Intestine Cellular Localization: Membrane

Human Small Intestine stained with anti-ZO-3 antibody E1745

Product Description: Tight junctions are complexes of proteins between the plasma membrane domains of epithelial and endothelial cells. ZO-3 forms complexes with ZO-1 and interacts with occludin and claudins. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.

108

Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting *Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details. **Product available to direct customers and Spring-appointed distributors only.


D E T E C T I O N A N D A N C I L L A RY P R O D U C T S

Spring Bioscience offers a complete line of detection systems optimized for immunohistochemistry (IHC) and are designed to meet the complex needs of any laboratory. Featuring: • Improved signal to noise ratios • Shorter incubation times • Works with mouse and rabbit primary antibodies • Clean and crisp staining

REVEAL™ Detection Systems – novel biotin-free chemistries enable our best-in-class detection systems, including: • REVEAL HRP DAB System • REVEAL HRP AEC System • REVEAL AP Red System


REVEAL™ Best-in-Class Detection Systems Spring Bioscience’s novel biotin-free detection system, REVEAL™, eliminates the polymer backbone and allows the enzyme to be conjugated directly to the secondary antibody by long-arm linkers. The compact size of the molecule enables diffusion through tissue promoting access to targeted binding sites.

p53 (SP5) Cat# M3054, Colon Adenocarcinoma

p53 (SP5) Cat #M3054, Colon Carcinoma

REVEAL AP Conjugate

REVEAL HRP Conjugate

Leading Competitor’s AP Conjugate

Leading Competitor’s HRP Polymer

Spring REVEAL Biotin-Free Detection Systems Catalog Number

110

Product Name

Size

Format

15ml

Ready-to-use

SPA-015

Reveal - Biotin-Free Polyvalent AEC

SPA-060

Reveal - Biotin-Free Polyvalent AEC

60ml

Ready-to-use

SPA-125

Reveal - Biotin-Free Polyvalent AEC

125ml

Ready-to-use

SPD-015

Reveal - Biotin-Free Polyvalent DAB

15ml

Ready-to-use

SPD-060

Reveal - Biotin-Free Polyvalent DAB

60ml

Ready-to-use

SPD-125

Reveal - Biotin-Free Polyvalent DAB

125ml

Ready-to-use

PAR-015

Reveal - Biotin-Free Polyvalent AP (Red)

15ml

Ready-to-use

PAR-060

Reveal - Biotin-Free Polyvalent AP (Red)

60ml

Ready-to-use

PAR-125

Reveal - Biotin-Free Polyvalent AP (Red)

125ml

Ready-to-use

SRA-015

Reveal - Rabbit-Specific AEC

15ml

Ready-to-use

SRA-060

Reveal - Rabbit-Specific AEC

60ml

Ready-to-use

SRA-125

Reveal - Rabbit-Specific AEC

125ml

Ready-to-use

SRD-015

Reveal - Rabbit-Specific DAB

15ml

Ready-to-use

SRD-060

Reveal - Rabbit-Specific DAB

60ml

Ready-to-use

SRD-125

Reveal - Rabbit-Specific DAB

125ml

Ready-to-use

RAR-015

Reveal - Rabbit Specific AP (Red)

15ml

Ready-to-use

RAR-060

Reveal - Rabbit Specific AP (Red)

60ml

Ready-to-use

RAR-125

Reveal - Rabbit Specific AP (Red)

125ml

Ready-to-use

MAR-015

Reveal - Mouse Specific AP (Red)

15ml

Ready-to-use

MAR-060

Reveal - Mouse Specific AP (Red)

60ml

Ready-to-use

MAR-125

Reveal - Mouse Specific AP (Red)

125ml

Ready-to-use

SPB-999

Reveal - Polyvalent HRP

1000ml

Ready-to-use

Spring Bioscience offers a complete line of detection systems optimized for immunohistochemistry (IHC) and are designed to meet the complex needs of any laboratory.


Detection and Ancillary Products Biotin-Streptavidin Detection Systems Catalog Number

Product Name

Size

Format

TPA-015

Polyvalent HRP AEC Detection System

15ml

Ready-to-use

TPD-015

Polyvalent HRP DAB Detection System

15ml

Ready-to-use

TRA-015

Anti-rabbit HRP AEC Detection System

15ml

Ready-to-use

TRD-015

Anti-rabbit HRP DAB Detection System

15ml

Ready-to-use

TMA-015

Anti-mouse HRP AEC Detection System

15ml

Ready-to-use

TMD-015

Anti-mouse HRP DAB Detection System

15ml

Ready-to-use

HRP-060

Polyvalent HRP Kit

60ml

Ready-to-use

HRP-125

Polyvalent HRP Kit

125ml

Ready-to-use

HRP-999

Polyvalent HRP Kit

1000ml

Ready-to-use

UVX-060

Polyvalent HRP Plus Kit

60ml

Ready-to-use

UVX-125

Polyvalent HRP Plus Kit

125ml

Ready-to-use

UVP-060

Polyvalent Alk Phos Kit

60ml

Ready-to-use

UVP-125

Polyvalent Alk Phos Kit

125ml

Ready-to-use

Size

Format

Detection Components Catalog Number

Product Name

DGP-125

Biotinylated Goat Polyvalent IgG's

125ml

Ready-to-use

DGP-999

Biotinylated Goat Polyvalent IgG's

1000ml

Ready-to-use

DGR-125

Biotinylated Goat anti-Rabbit IgG's

125ml

Ready-to-use

DGM-125

Biotinylated Goat anti-Mouse IgG's

125ml

Ready-to-use

DSA-125

Streptavidin Alkaline Phosphatase

125ml

Ready-to-use

DSP-125

Streptavidin Horseradish Peroxidase

125ml

Ready-to-use

DSP-999

Streptavidin Horseradish Peroxidase

1000ml

Ready-to-use

Diluent/Buffers/Miscellaneous Catalog Number

Product Name

Size

Format

125ml

Ready-to-use

ADS-125

Antibody Diluent

PBS-125

PBS (25x) pH 7.6

125ml

Concentrate

PBS-999

PBS (25x) pH 7.6

1000ml

Concentrate

PBT-125

PBS with Tween 20 (20x)

125ml

Concentrate

PBT-999

PBS with Tween 20 (20x)

1000ml

Concentrate

TBS-125

TBS (25x), pH7.4

125ml

Concentrate

TBS-999

TBS (25x), pH7.4

1000ml

Concentrate

TBT-125

TBS with Tween 20 (20x)

125ml

Concentrate

TBT-999

TBS with Tween 20 (20x)

1000ml

Concentrate

DMM-060

Mounting Media

60ml

Ready-to-use

DMM-125

Mounting Media

125ml

Ready-to-use

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

International Orders Email: intlorders@springbio.com

111


Epitope Recovery/Pretreatment Products Catalog Number

Product Name

Size

Format

PMB1-125

Antigen Retrieval Buffer 1 (100x) pH=6.0

125ml

Concentrate

PMB1-250

Antigen Retrieval Buffer 1 (100x) pH=6.0

250ml

Concentrate

PMB2-125

Antigen Retrieval Buffer 2 (100x) pH=8.0

125ml

Concentrate

PMB2-250

Antigen Retrieval Buffer 2 (100x) pH=8.0

250ml

Concentrate

PMB3-125

Antigen Retrieval Buffer 3 (100x) pH=10.0

125ml

Concentrate

PMB3-250

Antigen Retrieval Buffer 3 (100x) pH=10.0

250ml

Concentrate

PMB4-125

Antigen Retrieval Buffer 4 (100x) pH=9.0

125ml

Concentrate

PMB4-250

Antigen Retrieval Buffer 4 (100x) pH=9.0

250ml

Concentrate

DCB-125

Citrate Buffer (10x) pH 6.0

125ml

Concentrate

DCBX-050

Citrate Buffer (100x) pH 6.0

50ml

Concentrate

DEB-125

EDTA Buffer (10x) pH 8.0

125ml

Concentrate

DEBX-050

EDTA Buffer (100x) pH 8.0

50ml

Concentrate

DTB-125

Tris Buffer (10x) pH 10.0

125ml

Concentrate

DTBX-050

Tris Buffer (100x) pH 10.0

50ml

Concentrate

DTS-007

Trypsin (2 components)

7ml

Concentrate

DPS-007

Pepsin

7ml

Ready-to-use

PRK-004

Proteinase K

4ml

Ready-to-use

Blocking Reagents Catalog Number

Product Name

Size

Format

DHP-060

Hydrogen Peroxide Block

60ml

Ready-to-use

DHP-125

Hydrogen Peroxide Block

125ml

Ready-to-use

DPB-060

Protein Block

60ml

Ready-to-use

DPB-125

Protein Block

125ml

Ready-to-use

BRB-050

Background Reducing Buffer

50ml

Ready-to-use

DBB-015

Avidin/Biotin Blocking System (2 components)

15ml

Ready-to-use

Size

Format

Chromogens Catalog Number

112

Product Name

ASS-060

AEC Single Solution

60ml

Ready-to-use

ASS-125

AEC Single Solution

125ml

Ready-to-use

DAB-060

DAB (2 components, 50x)

60ml

Concentrate

DAB-125

DAB (2 components, 50x)

125ml

Concentrate

DAB-999

DAB (2 components, 50x)

1000ml

Concentrate

DABD-999

DAB (2 components, 25X)

1000ml

Concentrate

LFR-060

Liquid Fast Red Substrate System (75x)

60ml

Concentrate

LFR-125

Liquid Fast Red Substrate System (75x)

125ml

Concentrate

LFR-999

Liquid Fast Red Substrate System (75x)

1000ml

Concentrate

LFRD-999

Liquid Fast Red Substrate System (25x)

1000ml

Concentrate


A N T I B O D Y M I C R O A R R AY P R O D U C T S

Antibody microarrays provide researchers the convenience of detecting and analyzing multiple proteins simultaneously on a single slide. Formats Include: • Master Array (650+ antibodies) •

Arrays by Pathway (Angiogenesis, Apoptosis, Cancer Marker, Cell Cycle, Cytokine Array, Hematopoiesis, Hormone, Signal Transduction, and Stem Cell)

Research Applications Include: • Protein expression profiling • Comparison profiles of normal, diseased, or treated samples • Identification of candidate biomarkers Researcher Benefits Include: • Time savings • Reduction in reagent costs • Reduced variability • Flexible sample preparations (cell extracts, tissue lysates, treated samples) • Built-in control


Array Options to Meet Your Varied Research Interests Antibody microarrays provide a high-throughput platform for sensitive, efficient and accurate protein expression profiling, screening and comparison between normal and diseased or treated samples. Proteins from cell extracts, tissue lysates or treated samples may be used for analysis and detection. Antibody microarrays enable the qualitative measurement of protein expression. Spring’s Master Antibody Microarray kit consists of a set of two slides containing over 650 antibodies printed in duplicate. These high affinity antibodies are well characterized for immunohistochemistry or Western blot. Spring’s antibody microarrays by pathway are designed for researchers interested in a specific field such as cancer markers. Each pathway microarray kit contains two identical subarrays (one subarray for normal sample and another for the treated sample). Each subarray contains 6 replicates of each antibody. Positive and negative controls are included on each subarray. New Master Array coming in 2012!

Microarray Products Catalog Number

114

Product Name

Size

AMD-001

Antibody Microarray Detection Kit

AMS-700

Master Antibody Microarray Slides (656 Abs)

2 slides

1 kit

AAG-100

Angiogenesis Microarray Slides (127 Abs)

2 slides

AAP-100

Apoptosis Microarray Slides (71 Abs)

2 slides

ACC-100

Cell Cycle Microarray Slides (60 Abs)

2 slides

ACK-100

Cytokine Microarray Slides (77 Abs)

2 slides

ACM-100

Cancer Marker Microarray Slides (86 Abs)

2 slides

AHM-100

Hormone Microarray Slides (30 Abs)

2 slides

AHP-100

Hematopoiesis Microarray Slides (50 Abs)

2 slides

ASC-100

Stem Cell Microarray Slides (58 Abs)

2 slides

AST-100

Signal Transduction Microarray Slides (165 Abs)

2 slides

Antibody microarrays provide researchers the convenience of detecting and analyzing multiple proteins simultaneously on a single slide.


RECOMBINANT PROTEIN PRODUCTS

Recombinant proteins – expressed in E. coli and are: • Full length or partial sequence of specific proteins • Fused at N-terminus with GST/His-tag • Analyzed by SDS-PAGE using coomassie Blue staining • Available in 0.1ml (0.1mg/ml) size


116

Catalog Number

Product Name

Domain

P1094

ALK/p80

aa 426-528

P7014

BCL-6

aa 1-150

P3464

Cadherin E

aa 600-707

P1174

Calretinin

full length

P2824

Cathepsin D

full length

P3044

CD2

aa 25-209

P7044

CD4

aa 26-226

P7979

CD4

aa 207-316

P3024

CD23

aa 48-248

P7122

CD34

full length

P7054

CD68

full length

P3394

CD99

full length

P7982

CD138

aa 18-218

P2444

c-erbB2

aa 537-636

P1524

Chromogranin A

aa 260-454

P7978

c-Met

aa 18-217

P2434

D4GDI

full length

P1644

ER alpha

aa 1-300

P7977

ERCC1

full length

P3644

FHIT

full length

P7127

gp100

aa 1-150

P7113

GST

full length

P7513

IGF-1R

aa 1213-1367

P7861

Involucrin

aa 420-578

P7126

IRF4/MUM1

aa 144-451

P3264

Keratin 8

full length

P7514

LYVE-1

aa 243-323

P7120

MAGE-1

full length

P7522

NY-ESO-1

full length

P7074

p53 mutant (aa135)

full length

P2014

p53 wild type

full length

P2854

PCNA

full length

P3344

PGP9.5

full length

P7125

PPAR gamma

aa 1-477

P2074

Progesterone Receptor (PR)

aa 412-562

P7935

PSMA

aa 1-150

P7094

PTEN

full length

P2144

S100 beta

full length

P7121

S100A4

full length

P7914

SOX9

aa 1-150

P7874

TGF beta1

aa 177-391

P3354

Ubiquitin

full length

P2944

Vimentin

full length


Product Index

RbMab – SP Clones Actin-beta Rabbit Monoclonal 12 ALK/p80 Rabbit Monoclonal 14 Androgen Receptor (AR) Rabbit Monoclonal 15 BAX Rabbit Monoclonal 16 BCL-2 alpha Rabbit Monoclonal 16 BOB-1 Rabbit Monoclonal 18 C4d Rabbit Monoclonal 19 Cadherin-E/E-Cadherin Rabbit Monoclonal 19 Cadherin-N/N-Cadherin Rabbit Monoclonal 20 Calcitonin Rabbit Monoclonal 20 Calpain Rabbit Monoclonal 21 Calpastatin Rabbit Monoclonal 21 Calretinin Rabbit Monoclonal 22 Carbonic Anhydrase 9 (CA-9) Rabbit Monoclonal 22 Caveolin-1 Rabbit Monoclonal 24 CD3 Rabbit Monoclonal 25 CD4 Rabbit Monoclonal 26 CD5 Rabbit Monoclonal 26 CD7 Rabbit Monoclonal 26 CD8 Rabbit Monoclonal 27 CD10 Rabbit Monoclonal 27 CD19 Rabbit Monoclonal 27 CD20 Rabbit Monoclonal 28 CD22 Rabbit Monoclonal 28 CD23 Rabbit Monoclonal 28 CD31 Rabbit Monoclonal 29 CD43 Rabbit Monoclonal 30 CD44 Std Rabbit Monoclonal 30 CD79a Rabbit Monoclonal 32 CD99 Rabbit Monoclonal 33 CD117/c-kit Rabbit Monoclonal 33 CD163 Rabbit Monoclonal 34 CD278/ICOS Rabbit Monoclonal 35

Ki-67 Rabbit Monoclonal 66 LMO2 Rabbit Monoclonal 68 MCM2 Rabbit Monoclonal 69 Mesothelin Rabbit Monoclonal 71 MSH2 Rabbit Monoclonal 73 MSH6 Rabbit Monoclonal 74 MUM1/IRF4 Rabbit Monoclonal 76 Myeloperoxidase (MPO) Rabbit Monoclonal 76 Nestin Rabbit Monoclonal 77 Nitric Oxide Synthase-Inducible (iNOS) Rabbit Monoclonal 78 p120 (catenin delta 1) Rabbit Monoclonal 80 p53 Rabbit Monoclonal 81 p57 Rabbit Monoclonal 81 PAX-5 Rabbit Monoclonal 83 PI3K p85 Rabbit Monoclonal 85 PLAP Rabbit Monoclonal 86 Progesterone Receptor (PR) Rabbit Monoclonal 88 Progesterone Receptor (PR) Rabbit Monoclonal 88 PSMA Rabbit Monoclonal 90 S6 Ribosomal (Phospho S235/236) Rabbit Monoclonal 93 S6 Ribosomal (Phospho S240/244) Rabbit Monoclonal 93 SLIT2 Rabbit Monoclonal 94 SOX-2 Rabbit Monoclonal 96 Stathmin Rabbit Monoclonal 98 Survivin Rabbit Monoclonal 98 Synaptophysin Rabbit Monoclonal 99 Syntaxin 1A Rabbit Monoclonal 99 Tau (Neuronal Marker) Rabbit Monoclonal 100 Thymidylate Synthase (TS) Rabbit Monoclonal 101 Uroplakin III Rabbit Monoclonal 105 Vascular Endothelial Growth Factor (VEGF) Rabbit Monoclonal 106 Vimentin Rabbit Monoclonal 106 Vinculin Rabbit Monoclonal 107

CDK2 Rabbit Monoclonal 35 CDX2 Rabbit Monoclonal 36 c-erbB-2/HER-2 Rabbit Monoclonal 37 c-erbB-3/HER-3 Rabbit Monoclonal 37 Chromogranin A Rabbit Monoclonal 38 c-Met Rabbit Monoclonal 40 c-Met (Paired 1003) Rabbit Monoclonal 40 c-Met (Phospho Y1003) Rabbit Monoclonal 40 COX-2 Rabbit Monoclonal 42 c-TEN Rabbit Monoclonal 42 Cyclin D1 Rabbit Monoclonal 43 DOG-1 Rabbit Monoclonal 45 Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal 47 Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal 47 ERCC1 Rabbit Monoclonal 48 Estrogen Receptor (ER) Rabbit Monoclonal 49 F4/80 Rabbit Monoclonal 49 FLK-1 (mouse) Rabbit Monoclonal 51 FOXA1 Rabbit Monoclonal 52 FOXP3 Rabbit Monoclonal 53 Glial Fibrillary Acidic Protein (GFAP) Rabbit Monoclonal 54 Glypican-3 Rabbit Monoclonal 56 hENT1 Rabbit Monoclonal 59 IKAROS Rabbit Monoclonal 60 J-chain Rabbit Monoclonal 62 Keratin 5 Rabbit Monoclonal 62 Keratin 6 Rabbit Monoclonal 63 Keratin 7 Rabbit Monoclonal 63 Keratin 8 Rabbit Monoclonal 63 Keratin 10 Rabbit Monoclonal 64 Keratin 14 Rabbit Monoclonal 64 Keratin 17 Rabbit Monoclonal 64 Keratin 18 Rabbit Monoclonal 65 Keratin 20 Rabbit Monoclonal 66

14-3-3 beta Rabbit Polyclonal 12 14-3-3 zeta Rabbit Polyclonal 12

Annexin VI Rabbit Polyclonal 16 Apoptosis Protease Activating Factor 1 (APAF-1) Rabbit Polyclonal 16

A

B

Actin-beta Rabbit Monoclonal 12 Actin-beta Rabbit Polyclonal 12 Actin-Skeletal Muscle Rabbit Polyclonal 12 Actin-Skeletal Muscle Mouse Monoclonal 12 Actin-Smooth Muscle Rabbit Polyclonal 13 Actin-Smooth Muscle Mouse Monoclonal 13 Adenomatous Polyposis Coli (APC) Rabbit Polyclonal 13 Adrenocorticotrophic Hormone (ACTH) Rabbit Polyclonal 13 Adrenocorticotrophic Hormone (ACTH) Mouse Monoclonal 13 AKT-1/AKT-2 Rabbit Polyclonal 13 AKT-1/PKB (Phospho S473) Rabbit Polyclonal 14 ALK/p80 Rabbit Monoclonal 14 ALK/p80 Rabbit Polyclonal 14 alpha Fetoprotein (AFP) Rabbit Polyclonal 14 alpha-1-Antichymotrypsin Rabbit Polyclonal 14 alpha-1-Antitrypsin Rabbit Polyclonal 14 Amylin Peptide Rabbit Polyclonal 15 Amyloid Precursor Protein (APP) Rabbit Polyclonal 15 Amyloid-beta/Amyloid A4 Rabbit Polyclonal 15 Androgen Receptor (AR) Rabbit Monoclonal 15 Androgen Receptor (AR) Rabbit Polyclonal 15 Annexin I Rabbit Polyclonal 15

BAK Rabbit Polyclonal 16 BAX Rabbit Monoclonal 16 BAX Rabbit Polyclonal 16 BCL-2 alpha Rabbit Monoclonal 16 BCL-2 alpha Rabbit Polyclonal 17 BCL-6 Rabbit Polyclonal 17 BCL-10 Rabbit Polyclonal 17 BCL-10 Rabbit Polyclonal 17 BCL-10 Mouse Monoclonal 17 BCL-X Rabbit Polyclonal 17 BCL-XL Rabbit Polyclonal 18 BCL-XL Mouse Monoclonal 18 BIM/BOD Rabbit Polyclonal 18 Bnip3L Rabbit Polyclonal 18 BOB-1 Rabbit Monoclonal 18 BOB-1 Rabbit Polyclonal 18 Bone Morphogenetic Protein 4 (BMP-4) Rabbit Polyclonal 19

C C4d Rabbit Monoclonal 19 C4d Rabbit Polyclonal 19

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

c-Abl Rabbit Polyclonal 19 Cadherin-E/E-Cadherin Rabbit Monoclonal 19 Cadherin-E/E-Cadherin Mouse Monoclonal 19 Cadherin-E/E-Cadherin Rabbit Polyclonal 20 Cadherin-N/N-Cadherin Rabbit Monoclonal 20 Cadherin-N/N-Cadherin Rabbit Polyclonal 20 Cadherin-pan Rabbit Polyclonal 20 Calbindin D28 Rabbit Polyclonal 20 Calcitonin Rabbit Monoclonal 20 Calcitonin Rabbit Polyclonal 21 Calcium Sensing Receptor (CaSR) Rabbit Polyclonal 21 Calpain Rabbit Monoclonal 21 Calpain Rabbit Polyclonal 21 Calpastatin Rabbit Monoclonal 21 Calpastatin Rabbit Polyclonal 21 Calretinin Rabbit Monoclonal 22 Calretinin Rabbit Polyclonal 22 CaM Kinase IV Rabbit Polyclonal 22 CaM Kinase Kinase alpha Rabbit Polyclonal 22 Carbonic Anhydrase 9 (CA-9) Rabbit Monoclonal 22 Carbonic Anhydrase 9 (CA-9) Rabbit Polyclonal 22 CAS Rabbit Polyclonal 23 Caspase-1 Rabbit Polyclonal 23 Caspase-8 Rabbit Polyclonal 23 Caspase-10 Rabbit Polyclonal 23 Catenin-beta Rabbit Polyclonal 23

International Orders Email: intlorders@springbio.com

117


Catenin-gamma Rabbit Polyclonal 23 Cathepsin D Rabbit Polyclonal 24 Caveolin-1 Rabbit Monoclonal 24 Caveolin-1 Rabbit Polyclonal 24 Caveolin-1 Rabbit Polyclonal 24 CBP/p300-Interacting Transactivator 1 (CITED-1) Rabbit Polyclonal 24 CCR7 Rabbit Polyclonal 24 CCR8 Rabbit Polyclonal 25 CD1 alpha Rabbit Polyclonal 25 CD2 Rabbit Polyclonal 25 CD3 Rabbit Monoclonal 25 CD3 Rabbit Polyclonal 25 CD3 zeta Rabbit Polyclonal 25 CD4 Rabbit Monoclonal 26 CD4 Rabbit Polyclonal 26 CD5 Rabbit Monoclonal 26 CD5 Rabbit Polyclonal 26 CD7 Rabbit Monoclonal 26 CD7 Rabbit Polyclonal 26 CD8 Rabbit Monoclonal 27 CD8 Rabbit Polyclonal 27 CD9 Rabbit Polyclonal 27 CD10 Rabbit Monoclonal 27 CD10 Rabbit Polyclonal 27 CD19 Rabbit Monoclonal 27 CD19 Rabbit Polyclonal 28 CD20 Rabbit Monoclonal 28 CD20 Rabbit Polyclonal 28 CD22 Rabbit Monoclonal 28 CD22 Rabbit Polyclonal 28 CD23 Rabbit Monoclonal 28 CD23 Rabbit Polyclonal 29 CD30 Rabbit Polyclonal 29 CD31 Rabbit Monoclonal 29 CD31 Rabbit Polyclonal 29 CD34 (Endothelial Cell Marker) Rabbit Polyclonal 29 CD34 (Endothelial Cell Marker) Mouse Monoclonal 29 CD38 Rabbit Polyclonal 30 CD40 Rabbit Polyclonal 30 CD43 Rabbit Monoclonal 30 CD43 Rabbit Polyclonal 30 CD44 Std Rabbit Monoclonal 30 CD44 Std Rabbit Polyclonal 30 CD44 Std Mouse Monoclonal 31 CD45/T200/LCA Mouse Monoclonal 31 CD45RA Mouse Monoclonal 31 CD56 Mouse Monoclonal 31 CD56 Mouse Monoclonal 31 CD63 Mouse Monoclonal 31 CD68 Rabbit Polyclonal 32 CD68 Rabbit Polyclonal 32 CD68 Rabbit Polyclonal 32 CD74 Mouse Monoclonal 32 CD79a Rabbit Monoclonal 32 CD79a Rabbit Polyclonal 32 CD95/FAS Rabbit Polyclonal 33 CD99 Rabbit Monoclonal 33 CD99/MIC2 Rabbit Polyclonal 33 CD105/Endoglin/TGF 1/3 Receptor Rabbit Polyclonal 33 CD117/c-kit Rabbit Monoclonal 33 CD117/c-kit Rabbit Polyclonal 33 CD138 Rabbit Polyclonal 34 CD146 Rabbit Polyclonal 34

118

CD150 Rabbit Polyclonal 34 CD163 Rabbit Monoclonal 34 CD163 Rabbit Polyclonal 34 CD168 Rabbit Polyclonal 34 CD274/B7-H1 Rabbit Polyclonal 35 CD278/ICOS Rabbit Monoclonal 35 CD278/ICOS Rabbit Polyclonal 35 CDC2 p34 Rabbit Polyclonal 35 CDH1 Rabbit Polyclonal 35 CDK2 Rabbit Monoclonal 35 CDK2 Rabbit Polyclonal 36 CDX2 Rabbit Monoclonal 36 CDX2 Rabbit Polyclonal 36 CEA/CD66e Rabbit Polyclonal 36 CEA/CD66e Mouse Monoclonal 36 c-erbB-2 (Paired 1248) Rabbit Polyclonal 36 c-erbB-2 (Phospho Y1248) Rabbit Polyclonal 37 c-erbB-2/HER-2 Rabbit Monoclonal 37 c-erbB-2/HER-2 Rabbit Polyclonal 37 c-erbB-3/HER-3 Rabbit Monoclonal 37 c-erbB-3/HER-3 Rabbit Polyclonal 37 c-erbB-3/HER-3 Rabbit Polyclonal 37 c-erbB-4/HER-4 Rabbit Polyclonal 38 c-Fos Rabbit Polyclonal 38 Cholecystokinin (CCK-8) Rabbit Polyclonal 38 Chromogranin A Rabbit Monoclonal 38 Chromogranin A Rabbit Polyclonal 38 Claudin-1 Rabbit Polyclonal 38 Claudin-3 Rabbit Polyclonal 39 Claudin-4 Rabbit Polyclonal 39 Claudin-5 Rabbit Polyclonal 39 Claudin-7 Rabbit Polyclonal 39 Claudin-10 Rabbit Polyclonal 39 Claudin-11 Rabbit Polyclonal 39 c-Met Rabbit Monoclonal 40 c-Met Rabbit Polyclonal 40 c-Met (Paired 1003) Rabbit Monoclonal 40 c-Met (Paired 1003) Rabbit Polyclonal 40 c-Met (Paired 1349) Rabbit Polyclonal 40 c-Met (Phospho Y1003) Rabbit Monoclonal 40 c-Met (Phospho Y1003) Rabbit Polyclonal 41 c-Met (Phospho Y1234/1235) Rabbit Polyclonal 41 c-Met (Phospho Y1349) Rabbit Polyclonal 41 Connexin-43 Rabbit Polyclonal 41 Corticotropin Releasing Factor (CRF) Rabbit Polyclonal 41 COX-1 Rabbit Polyclonal 41 COX-2 Rabbit Monoclonal 42 COX-2 Rabbit Polyclonal 42 CREB-Binding Protein (CBP) Rabbit Polyclonal 42 Cripto-1 Rabbit Polyclonal 42 c-TEN Rabbit Monoclonal 42 c-TEN Rabbit Polyclonal 42 Cullin-1 (CUL-1) Rabbit Polyclonal 43 Cullin-2 (CUL-2) Rabbit Polyclonal 43 CXCR4 Rabbit Polyclonal 43 Cyclin B1 Rabbit Polyclonal 43 Cyclin D1 Rabbit Monoclonal 43 Cyclin D1 Rabbit Polyclonal 43 Cyclin E Rabbit Polyclonal 44 CYR61 Rabbit Polyclonal 44 Cytochrome C Rabbit Polyclonal 44

D D4-GDI Rabbit Polyclonal 44 D5DR Rabbit Polyclonal 44 DAPK2 Rabbit Polyclonal 44 Desmin Rabbit Polyclonal 45 DFF-45 Rabbit Polyclonal 45 DHFR Rabbit Polyclonal 45 DJ-1 Rabbit Polyclonal 45 DOG-1 Rabbit Monoclonal 45 DOG-1 Rabbit Polyclonal 45 DOK-1 Rabbit Polyclonal 46 DOK-2 Rabbit Polyclonal 46 DRAK2 Rabbit Polyclonal 46 Dysferlin Rabbit Polyclonal 46 Dystrophin Rabbit Polyclonal 46 Dystrophin Mouse Monoclonal 46

E E2F-2 Rabbit Polyclonal 47 EGFL7 Rabbit Polyclonal 47 Ephrin A4 Rabbit Polyclonal 47 Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal 47 Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal 47 Epidermal Growth Factor Receptor (EGFR) Mouse Monoclonal 47 Epidermal Growth Factor Receptor (EGFR) Rabbit Polyclonal 48 ERAB Rabbit Polyclonal 48 ERCC1 Rabbit Monoclonal 48 ERCC1 Rabbit Polyclonal 48 ERCC1 Rabbit Polyclonal 48 ERK-1 Rabbit Polyclonal 48 ERK-2 Rabbit Polyclonal 49 ESA/Ep-CAM Mouse Monoclonal 49 Estrogen Receptor (ER) Rabbit Monoclonal 49 Estrogen Receptor (ER) Rabbit Polyclonal 49 EZH2 Rabbit Polyclonal 49

F F4/80 Rabbit Monoclonal 49 F4/80 Rabbit Polyclonal 50 FAS Ligand Rabbit Polyclonal 50 FAS-Associated Phosphatase (FAP-1) Rabbit Polyclonal 50 FcRH5 Rabbit Polyclonal 50 FGFR-1 Rabbit Polyclonal 50 FGFR-2 Rabbit Polyclonal 50 FGFR-3 Rabbit Polyclonal 51 FLI-1 Rabbit Polyclonal 51 FLK-1 (mouse) Rabbit Monoclonal 51 FLK-1/KDR/VEGFR-2 Rabbit Polyclonal 51 FLT-1/VEGFR1 Rabbit Polyclonal 51 FLT-4/VEGFR3 Rabbit Polyclonal 51 Fn14 Rabbit Polyclonal 52 FOXA1 Rabbit Monoclonal 52 FOXA1 Rabbit Polyclonal 52 FOXO1A Rabbit Polyclonal 52 FOXO3A Rabbit Polyclonal 52 FOXP1 Rabbit Polyclonal 52 FOXP3 Rabbit Monoclonal 53 FOXP3 Rabbit Polyclonal 53 FOXP4 Rabbit Polyclonal 53 FPGS Rabbit Polyclonal 53 FRA-2 Rabbit Polyclonal 53 Fragile Histidine Triad (FHIT) Rabbit Polyclonal 53


Product Index G GADD153 Rabbit Polyclonal 54 Gamma Glutamylcysteine Synthetase (GCS) Rabbit Polyclonal 54 GANP Rabbit Polyclonal 54 Gastrin-1 Rabbit Polyclonal 54 GDNF Rabbit Polyclonal 54 Glial Fibrillary Acidic Protein (GFAP) Rabbit Monoclonal 54 Glial Fibrillary Acidic Protein (GFAP) Rabbit Polyclonal 55 Glial Fibrillary Acidic Protein (GFAP) Mouse Monoclonal 55 Glucagon Rabbit Polyclonal 55 GluR1 Rabbit Polyclonal 55 GluR2/3 Rabbit Polyclonal 55 GluR6/7 Rabbit Polyclonal 55 GLUT-1 Rabbit Polyclonal 56 GLUT-1 Mouse Monoclonal 56 GLUT-3 Rabbit Polyclonal 56 Glutamic Acid Decarboxylase 65 (GAD65) Rabbit Polyclonal 56 Glutamic Acid Decarboxylase 67 (GAD67) Rabbit Polyclonal 56 Glypican-3 Rabbit Monoclonal 56 Glypican-3 Rabbit Polyclonal 57 GNB2 Rabbit Polyclonal 57 GP200 (Renal Cell Carcinoma Marker) Mouse Monoclonal 57 Granzyme B Rabbit Polyclonal 57 GRB2-Associated Binder 1 (GAB-1) Rabbit Polyclonal 57 GRP75 Rabbit Polyclonal 57 GRP78 Rabbit Polyclonal 58 GRP94 Rabbit Polyclonal 58 GRSF-1 Rabbit Polyclonal 58 GSK-3 Rabbit Polyclonal 58 Guanylyl Cyclase beta Rabbit Polyclonal 58

H H. Pylori Rabbit Polyclonal 58 H. Pylori Mouse Monoclonal 59 HDAC1 Rabbit Polyclonal 59 HDAC2 Rabbit Polyclonal 59 Heat Shock Protein 60 (HSP60) Rabbit Polyclonal 59 Heat Shock Protein 70 (HSP70) Rabbit Polyclonal 59 hENT1 Rabbit Monoclonal 59 HMGA2 Rabbit Polyclonal 60 Human Growth Hormone (HGH) Rabbit Polyclonal 60

I ID2 Rabbit Polyclonal 60 ID3 Rabbit Polyclonal 60 IKAROS Rabbit Monoclonal 60 IKAROS Rabbit Polyclonal 60 Inhibin-alpha Rabbit Polyclonal 61 Integrin beta 5 Rabbit Polyclonal 61 Involucrin Rabbit Polyclonal 61 IPO-38 (Proliferation Marker) Mouse Monoclonal 61 IRS-1 Rabbit Polyclonal 61

J JAB1 Rabbit Polyclonal 61 J-chain Rabbit Monoclonal 62 J-chain Rabbit Polyclonal 62 Jena-Muenchen4 (JM4) Rabbit Polyclonal 62

K Kappa Light Chain (B Cell Marker) Mouse Monoclonal 62 Keratin 5 Rabbit Monoclonal 62

MMP-3 (Stromelysin-1) Rabbit Polyclonal 72 MMP-9 (92kDa Collagenase IV) Rabbit Polyclonal 72 MMP-10 (Stromelysin-2) Rabbit Polyclonal 73 MMP-14/MT1-MMP Rabbit Polyclonal 73 MMP-15/MT2-MMP Rabbit Polyclonal 73 MMP-19 Rabbit Polyclonal 73 MMP-23 Rabbit Polyclonal 73 MSH2 Rabbit Monoclonal 73 MSH2 Rabbit Polyclonal 74 MSH3 Rabbit Polyclonal 74 MSH6 Rabbit Monoclonal 74 MSH6 Mouse Monoclonal 74 MSH6 Rabbit Polyclonal 74 mTOR Rabbit Polyclonal 74 mTOR Rabbit Polyclonal 75 MUC-1 Underglycosylated Mouse Monoclonal 75 MUC-1/EMA/Epithelial Membrane Antigen Rabbit Polyclonal 75 MUC-1/EMA/Epithelial Membrane Antigen Mouse Monoclonal 75 Mucin 5AC Mouse Monoclonal 75 Mucin 5AC Mouse Monoclonal 75 Mucin-2/MUC-2 Mouse Monoclonal 76 MUM1/IRF4 Rabbit Monoclonal 76 MUM1/IRF4 Rabbit Polyclonal 76 Myeloperoxidase (MPO) Rabbit Monoclonal 76 Myeloperoxidase (MPO) Rabbit Polyclonal 76 Myoglobin Rabbit Polyclonal 76

Keratin 5 Rabbit Polyclonal 62 Keratin 6 Rabbit Monoclonal 63 Keratin 6 Rabbit Polyclonal 63 Keratin 7 Rabbit Monoclonal 63 Keratin 7 Rabbit Polyclonal 63 Keratin 8 Rabbit Monoclonal 63 Keratin 8 Rabbit Polyclonal 63 Keratin 8 Rabbit Polyclonal 64 Keratin 10 Rabbit Monoclonal 64 Keratin 10 Rabbit Polyclonal 64 Keratin 14 Rabbit Monoclonal 64 Keratin 14 Rabbit Polyclonal 64 Keratin 17 Rabbit Monoclonal 64 Keratin 17 Rabbit Polyclonal 65 Keratin 18 Rabbit Monoclonal 65 Keratin 18 Rabbit Polyclonal 65 Keratin 18 Mouse Monoclonal 65 Keratin 18 Mouse Monoclonal 65 Keratin 19 Rabbit Polyclonal 65 Keratin 20 Rabbit Monoclonal 66 Keratin 20 Rabbit Polyclonal 66 Ki-67 Rabbit Monoclonal 66 Ki-67 Rabbit Polyclonal 66 KOC/L523S Rabbit Polyclonal 66 k-Ras Rabbit Polyclonal 66 Ku80 Rabbit Polyclonal 67 Ku80 Rabbit Polyclonal 67

N

L Lambda Light Chain Rabbit Polyclonal 67 Lamin B Rabbit Polyclonal 67 Lewis A Mouse Monoclonal 67 LIMD1 Rabbit Polyclonal 67 Lipocalin-2 Rabbit Polyclonal 68 LMO2 Rabbit Monoclonal 68 LMO2 Rabbit Polyclonal 68 LMO2 Mouse Monoclonal 68 Luteinizing Hormone (LH) Rabbit Polyclonal 68

M MAGE-1 Rabbit Polyclonal 68 MALT-1 Rabbit Polyclonal 69 Mammaglobin Rabbit Polyclonal 69 MART-1/Melan-A Rabbit Polyclonal 69 MCL-1 Rabbit Polyclonal 69 MCM2 Rabbit Monoclonal 69 MCM2 Rabbit Polyclonal 69 MCM2 Rabbit Polyclonal 70 MCM5 Rabbit Polyclonal 70 MDGI Rabbit Polyclonal 70 MDGI Rabbit Polyclonal 70 MDM2 Rabbit Polyclonal 70 MEF-2 Rabbit Polyclonal 70 Melanoma (gp100) Rabbit Polyclonal 71 Mesothelin Rabbit Monoclonal 71 Mesothelin Rabbit Polyclonal 71 mGluR1 Rabbit Polyclonal 71 mGluR5 Rabbit Polyclonal 71 Microphthalmia (MiTF) Rabbit Polyclonal 71 MIP-1 alpha Rabbit Polyclonal 72 MLH1 Rabbit Polyclonal 72 MMP-1 Rabbit Polyclonal 72 MMP-2 (72kDa Collagenase IV) Rabbit Polyclonal 72

US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469

NCK2 Rabbit Polyclonal 77 Nephrin Rabbit Polyclonal 77 Nestin Rabbit Monoclonal 77 Nestin Rabbit Polyclonal 77 Nestin Rabbit Polyclonal 77 Neuron Specific Enolase (NSE) Rabbit Polyclonal 77 NFkB p50 Rabbit Polyclonal 78 NFkB p65 Rabbit Polyclonal 78 Nitric Oxide Synthase-Endothelial (eNOS) Rabbit Polyclonal 78 Nitric Oxide Synthase-Inducible (iNOS) Rabbit Monoclonal 78 Nitric Oxide Synthase-Inducible (iNOS) Rabbit Polyclonal 78 Nitric Oxide Synthase-Universal (uNOS) Rabbit Polyclonal 78 NKX3.1 Rabbit Polyclonal 79 Notch-1 Rabbit Polyclonal 79 NRF2 Rabbit Polyclonal 79

O Occludin Rabbit Polyclonal 79 OCT-1 Rabbit Polyclonal 79 OCT-2 Rabbit Polyclonal 79 OCT-3/OCT-4 Rabbit Polyclonal 80 OCT-3/OCT-4 Rabbit Polyclonal 80 Osteopontin Rabbit Polyclonal 80

P p120 (catenin delta 1) Rabbit Monoclonal 80 p120 (catenin delta 1) Rabbit Polyclonal 80 p14ARF Rabbit Polyclonal 80 p27Kip1 Rabbit Polyclonal 81 p300/CBP Rabbit Polyclonal 81 p53 Rabbit Monoclonal 81 p53 Rabbit Polyclonal 81 p53 Mouse Monoclonal 81 p57 Rabbit Monoclonal 81 p57 Rabbit Polyclonal 82

International Orders Email: intlorders@springbio.com

119


p70S6k Rabbit Polyclonal 82 p70S6k (Paired 421/424) Rabbit Polyclonal 82 p70S6k (Phospho T421/S424) Rabbit Polyclonal 82 PAK alpha Rabbit Polyclonal 82 Parathyroid Hormone Rabbit Polyclonal 82 Parathyroid Hormone Receptor 1 Rabbit Polyclonal 83 Parkin Rabbit Polyclonal 83 PAX-5 Rabbit Monoclonal 83 PAX-5 Rabbit Polyclonal 83 Paxillin Rabbit Polyclonal 83 PCNA Rabbit Polyclonal 83 PD-1/CD279 Rabbit Polyclonal 84 PD-1/CD279 Rabbit Polyclonal 84 PDCD6 Rabbit Polyclonal 84 PDGFR alpha Rabbit Polyclonal 84 PDGFR beta Rabbit Polyclonal 84 PDK-1 Rabbit Polyclonal 84 PDS-1/Securin Rabbit Polyclonal 85 Peripheral Myelin Protein (PMP-22) Rabbit Polyclonal 85 Phospho-Ser/Thr/Tyr Mouse Monoclonal 85 Phosphotyrosine Mouse Monoclonal 85 PI3K p110beta Rabbit Polyclonal 85 PI3K p85 Rabbit Monoclonal 85 PI3K p85 Rabbit Polyclonal 86 PIM-1 Rabbit Polyclonal 86 PIM-2 Rabbit Polyclonal 86 Placental Lactogen (hPL) Rabbit Polyclonal 86 PLAP Rabbit Monoclonal 86 PLAP Rabbit Polyclonal 86 PLC beta 1 Rabbit Polyclonal 87 PLC gamma 2 Rabbit Polyclonal 87 Pleiotrophin Rabbit Polyclonal 87 PML Rabbit Polyclonal 87 PMS2 Rabbit Polyclonal 87 Podocin Rabbit Polyclonal 87 Poly ADP-Ribose Polymerase (PARP) Rabbit Polyclonal 88 Potassium Channel Kv3.2 Rabbit Polyclonal 88 Presenilin Rabbit Polyclonal 88 Progesterone Receptor (PR) Rabbit Monoclonal 88 Progesterone Receptor (PR) Rabbit Monoclonal 88 Progesterone Receptor (PR) Rabbit Polyclonal 88 Prohibitin Rabbit Polyclonal 89 Prolactin Rabbit Polyclonal 89 Prostate Apoptosis Response 4 (PAR-4) Rabbit Polyclonal 89 Prostate Specific Antigen (PSA) Rabbit Polyclonal 89 Prostate Stem Cell Antigen (PSCA) Rabbit Polyclonal 89 Protein Gene Protein 9.5 (PGP9.5) Rabbit Polyclonal 89 PSMA Rabbit Monoclonal 90 PSMA Rabbit Polyclonal 90

R RACK-1 Rabbit Polyclonal 90 RAD51 Rabbit Polyclonal 90 RAN Rabbit Polyclonal 90 RCAS1 Rabbit Polyclonal 90 RCC1 Rabbit Polyclonal 91 Retinoblastoma (Rb) Rabbit Polyclonal 91 Retinoic Acid Receptor beta Rabbit Polyclonal 91 Retinoic X Receptor gamma Rabbit Polyclonal 91 RHO Rabbit Polyclonal 91 ROC1 Rabbit Polyclonal 91 RRM1 Rabbit Polyclonal 92

120

S S100 Rabbit Polyclonal 92 S100A4 Rabbit Polyclonal 92 S6 Ribosomal Rabbit Polyclonal 92 S6 Ribosomal (Paired 235/236) Rabbit Polyclonal 92 S6 Ribosomal (Paired 240/244) Rabbit Polyclonal 92 S6 Ribosomal (Phospho S235/236) Rabbit Monoclonal 93 S6 Ribosomal (Phospho S235/236) Rabbit Polyclonal 93 S6 Ribosomal (Phospho S240/244) Rabbit Monoclonal 93 S6 Ribosomal (Phospho S240/244) Rabbit Polyclonal 93 SHIP-1 Rabbit Polyclonal 93 SH-PTP2 Rabbit Polyclonal 93 SKP1 p19 Rabbit Polyclonal 94 SKP2 p45 Rabbit Polyclonal 94 SLIT2 Rabbit Monoclonal 94 SLIT2 Rabbit Polyclonal 94 SMA Rabbit Polyclonal 94 SMAD3 Rabbit Polyclonal 94 SMRTe Rabbit Polyclonal 95 SOCS-2 Rabbit Polyclonal 95 SOCS-3 Rabbit Polyclonal 95 SOCS-6 Rabbit Polyclonal 95 Sodium Channel-pan Rabbit Polyclonal 95 Sonic Hedgehog Rabbit Polyclonal 95 SOX-2 Rabbit Monoclonal 96 SOX-2 Rabbit Polyclonal 96 SOX-4 Rabbit Polyclonal 96 SOX-9 Rabbit Polyclonal 96 SOX-9 Rabbit Polyclonal 96 SOX-11 Rabbit Polyclonal 96 SOX-14 Rabbit Polyclonal 97 SP1 Rabbit Polyclonal 97 ST13 Rabbit Polyclonal 97 Stanniocalcin-2 Rabbit Polyclonal 97 STAT3 Rabbit Polyclonal 97 STAT5 alpha Rabbit Polyclonal 97 STAT6 Rabbit Polyclonal 98 Stathmin Rabbit Monoclonal 98 Stathmin Rabbit Polyclonal 98 Survivin Rabbit Monoclonal 98 Survivin Rabbit Polyclonal 98 SYK Rabbit Polyclonal 98 Synaptophysin Rabbit Monoclonal 99 Synaptophysin Rabbit Polyclonal 99 Syntaxin 1A Rabbit Monoclonal 99 Syntaxin 1A Rabbit Polyclonal 99 Synuclein-alpha Rabbit Polyclonal 99 Synuclein-beta Rabbit Polyclonal 99 Synuclein-pan Rabbit Polyclonal 100

T Tau (Neuronal Marker) Rabbit Monoclonal 100 Tau (Neuronal Marker) Rabbit Polyclonal 100 TdT Rabbit Polyclonal 100 Thioredoxin Reductase 2 (TrxR2) Rabbit Polyclonal 100 Thrombin R/ATAP2 Rabbit Polyclonal 100 Thymidylate Synthase (TS) Rabbit Monoclonal 101 Thymidylate Synthase (TS) Rabbit Polyclonal 101 Thymidylate Synthase (TS) Rabbit Polyclonal 101 Thyroglobulin Mouse Monoclonal 101 Thyroid Hormone Receptor alpha1/alpha2 Rabbit Polyclonal 101 Thyroid Hormone Receptor beta Rabbit Polyclonal 101

Thyroid Stimulating Hormone (TSH) Rabbit Polyclonal 102 TIE-1 Rabbit Polyclonal 102 TIMP-1 Rabbit Polyclonal 102 TIMP-2 Rabbit Polyclonal 102 TIMP-3 Rabbit Polyclonal 102 TNF-R2 Rabbit Polyclonal 102 Topo II beta Rabbit Polyclonal 103 Topoisomerase I Rabbit Polyclonal 103 Toxoplasma Gondii Rabbit Polyclonal 103 TRADD Rabbit Polyclonal 103 Transforming Growth Factor beta R3 Rabbit Polyclonal 103 Transforming Growth Factor beta1 (TGFb1) Rabbit Polyclonal 103 Transforming Growth Factor beta2 (TGFb2) Rabbit Polyclonal 104 Transforming Growth Factor beta3 (TGFb3) Rabbit Polyclonal 104 Transforming Growth Factor R1 Rabbit Polyclonal 104 Transglutaminase II Rabbit Polyclonal 104 TTF-1 Rabbit Polyclonal 104 TTF-1 Mouse Monoclonal 104 Tubulin-alpha Rabbit Polyclonal 105 Tubulin-beta Rabbit Polyclonal 105 Tubulin-gamma Rabbit Polyclonal 105

U Ubiquitin Rabbit Polyclonal 105 Urocortin Rabbit Polyclonal 105 Uroplakin III Rabbit Monoclonal 105 Uroplakin III Rabbit Polyclonal 106 USF-2 Rabbit Polyclonal 106

V Vascular Endothelial Growth Factor (VEGF) Rabbit Monoclonal 106 Vascular Endothelial Growth Factor (VEGF) Rabbit Polyclonal 106 Villin Rabbit Polyclonal 106 Vimentin Rabbit Monoclonal 106 Vinculin Rabbit Monoclonal 107 Vinculin Rabbit Polyclonal 107

W Wilm’s Tumor (WT) Rabbit Polyclonal 107 WNT-1 Rabbit Polyclonal 107

X XMRV Rabbit Polyclonal 107 XRCC2 Rabbit Polyclonal 107

Z ZAP-70 Rabbit Polyclonal 108 ZO-3 Rabbit Polyclonal 108



CORPORATE OFFICE 6920 Koll Center Parkway, Suite 211 Pleasanton, CA 94566 USA Email: info@springbio.com Telephone: +1.925.474.8440 Toll Free: 800.787.6896 Fax: +1.925.474.8469 www.springbio.com

US CUSTOMERS orders@springbio.com INTERNATIONAL CUSTOMERS intlorders@springbio.com TECHNICAL SUPPORT Telephone: +1.925.474.8479 Email: tech@springbio.com

For Research Use Only. Not for use in diagnostic procedures. Š2012, Spring Bioscience Corporation. Spring Bioscience, the logo and REVEAL are trademarks of Spring Bioscience Corporation. All rights reserved.


Turn static files into dynamic content formats.

Create a flipbook
Issuu converts static files into: digital portfolios, online yearbooks, online catalogs, digital photo albums and more. Sign up and create your flipbook.