Ceva-Vectra3D-PosterAntifeeding-0614:Mise en page 1 12/06/14 13:37 Page1
Immediate and residual anti-feeding efficacy of a dinotefuran-permethrinpyriproxyfen topical administration against sandfly (Phlebotomus perniciosus ) infested dogs under laboratory conditions Varloud M. 1, Warin S. 1, Murphy M. 3, Moran C. 3, McGrath S. 3, Ferrari G.* 2, 1
Ceva, 10 avenue de la Ballastière, 33500 Libourne, France. 2Ceva Salute Animale S.p.A, Viale Colleoni, 15 - 20864 Agrate Brianza (MB) Italy. 3Charles River Laboratories Preclinical Services Ireland Ltd, Ballina, Co. Mayo, Ireland.
Introduction Sandflies are hematophageous parasites that must perform a blood-meal for oviposition. Phlebotomus perniciosus is a European species of sandflies that can feed on dogs. It is known for transmitting Leishmania infantum. This study was conducted to
assess the anti-feeding efficacy of a dinotefuran-permethrinpyriproxyfen topical ectoparasiticide (DPP, Vectra® 3D) against non-blood fed adult female P. perniciosus infested in dogs under laboratory conditions.
Material & Methods DDP 1,6 mL > 4-10 kg BW 3,6 mL > 10-25 kg BW
85 ± 5 Infestations P. perniciosus 10 - 20 Days -6
Exposure chamber
85 ± 5 10 - 20
85 ± 5 10 - 20
85 ± 5 10 - 20
1
7
14
0
85 ± 5 10 - 20
60-65 min
85 ± 5 10 - 20
22
28
n = 14 dogs, sedated Untreated control (n=7, 10.2 ± 1.1 kg BW)
Blood fed
DPP (n=7, 10.5 ± 1.0 kg BW)
Unfed Amc= arithmetic mean number of blood fed flies in control
Amc - Amt Efficacy (%) = x 100 Amc
Amt= arithmetic mean number of blood fed flies in treated group
Results of engorged sandflies differed significantly (p<0.05) between control and treated groups at each time-point. 100
60 97,3
97,5 93,8
50
90
53 91,0 87,9 43
70
40 35
60
35
34
80
30
50 40 21
20
21
30 20
10 6 1
0 -6
1
1
7
4
1
14
10 0
22
Anti-feeding efficacy (arithmetic means, %)
Arithmetic mean number of fed flies 1h after infestation (7 dogs/group)
The DPP treatment provided ≥ 88% anti-feeding efficacy for the duration of the four week assessment period. The mean number
Control Vectra® 3D Efficacy
28
Time post-treatment (days)
Conclusion This study underlines the immediate and residual anti-feeding effect of DPP for one month against sandflies potentially challenging a
treated dog. Topical application of DPP at the recommended dose was well tolerated.