ADME Services GVK BIO In vitro ADME screening service offers a portfolio of automated High throughput assays for investigating:
Solubility
metabolism,
permeability,
properties. We determine equilibrium solubility by dried
solubility & physicochemical properties. We deliver consistent, accurate compound data with cost-efficiency.
DMSO method. We can also determine solubility in
distribution
and
toxicity,
In vitro ADME Capabilities •
Physicochemical studies
Solubility is one of the most important physicochemical
aqueous buffer (pH 1-9), organic solvents (DMSO, Ethanol, etc), formulations and excipients by pION / Multiscreen / HPLC - UV / MS / MS- MS methods.
Log D/Log P Solubility(Kinetic/Equilibrium) Chemical stability Biological matrix stability (serum / plasma / microsomes / blood / hepatocytes / tissue homogenates)
•
Absorption/Distribution Assays Caco2 and PAMPA permeability Tissue permeability studies- Franz Diffusion assay Pgp substrate / inhibitor assays Protein binding Blood/Plasma Partitioning Ratio
•
Metabolism/Excretion Half life / clearance determination using Microsomes / Hepatocytes / S9 fractions / CYP across species (Human /
Protein Binding In vitro binding plasma have
studies
proven
with
to be a
valuable tool for predicting In vivo protein binding. We determine the protein
binding
filtration
and
by
both
rapid
ultra
equilibrium
dialysis. Using RED device
we
determine
in
protein
binding
microsomes, plasma and tissue homogenate
across
various
Rat / Mouse / Dog / monkey)
species (Rat / Mouse / Human &
CYP Inhibition (CYP1A2, CYP2C9, CYP2C19, CYP2D6,
Dog).
CYP3A4 and 2C8) Pathway determination (Phase I and Phase II) Metabolite identification with Microsomes /
•
Hepatocytes and S9 fraction across species(Human / Rat / Mouse / Dog / Monkey) using Light Sight MetId
PAMPA (Parallel artificial membrane permeability assay)
software
The
Log D/Log P
Parallel Artificial Membrane
(PAMPA) is used
as
Permeability Assay
an in vitro model of passive,
Log D is determined by the shake-flask method, by
transcellular permeability and also for the prediction of
dissolving some of the solute in a volume of octanol and water/buffer and measure the concentration of the solute
oral
in each solvent. Log D is determined by HPLC-UV with confirmation by mass.
Multiscreen/
absorption
and
brain
permeability (log Pe) may
penetration. be
Effective
measured by pION/ LCMS.