OvaTube (Maravet)

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Centrifugal Flotation Device for In-clinic Parasite Detection

Background … • 20-25 million companion animal fecal exams are performed per year in U.S. StatSpin estimate based on industry sources

• Fully 1/3rd of U.S. dogs are currently infected with gastrointestinal parasites www.petsandparasites.org

(CAPC)

• Between 1-3 million people per year are infected with hookworm by their pets www.petsandparasites.org (CAPC)

• CAPC guidelines: 2-4 fecal exams in first year; 1-2 per year for adults

$

60 million+ pet dogs 70 million+ pet cats (AVMA Demographics 2008)

www.CAPCvet.org

Fecal flotation: a substantial and growing market opportunity!

200 million/year potential tests!

The Opportunity … “Failure to use best-practice techniques, such as centrifugation, when conducting fecal flotation procedures can result in failure to detect parasite stages …” www.CAPCvet.org

• Studies show that simple gravity devices have an unacceptably high number of false negatives • CAPC Guidelines recommend only centrifugal flotation be used – gravity devices are “sub-optimal” • Given the zoonotic potential of many parasites, accurate diagnosis is critical to the health of pets and pet owners

The Opportunity … “We stopped using the gravity device when I saw the studies. I decided to send fecals out because the centrifuge method takes too long to set up and it’s very messy.”

A typical Vet in our Market Research

• The traditional centrifuge method is slow, labor intensive and unpleasant • Many clinics send samples out, losing revenue, time and control – Sample degradation – Mass production/transcription errors – In clinic “stats” are impossible

Now you can offer your customers an innovative alternative for in-clinic parasite testing …

$8 to $12 per test

Introducing the …

Centrifugal Flotation Device for In-clinic Parasite Detection

• Centrifugal accuracy without the complexity and mess • Faster, far simpler method … diagnosis can be made while patient is in clinic • Better medicine for pets (and pet owners) … greater profits for the veterinarian An excellent recurring sales opportunity for you!

In-clinic Ova and Parasite Detection Mixer

Tube

Filter

SSOT-50 Dispenser box of 50 Kits

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Sample should be fresh ‌ < 1 hour or refrigerated

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Sample should be fresh ‌ < 1 hour or refrigerated • Press mixer into feces to collect 1 gram sample

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Sample should be fresh ‌ < 1 hour or refrigerated Long stem makes collection easier & more hygienic

U.S. and International Patents Pending

• Press mixer into feces to collect 1 gram sample

In-clinic Ova and Parasite Detection

• Add floatation fluid (e.g. zinc sulfate) to fill line on tube Common Floatation Fluids

Magnesium Sulfate

1.32

Sodium Chloride

1.20

Sodium Nitrate

Fill Line

U.S. and International Patents Pending

Specific Gravity

1.2-1.33

Sucrose

1.25

Zinc Sulfate

1.18

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with floatation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer • Remove the Mixer and discard

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Complete sample mixing and release of ova from fecal matter is achieved with far less effort and mess!

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while chopping and twisting the Mixer • Remove the Mixer and discard

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Filter into tube ‌ align arrow with notch at top of tube

Ramp pin is inserted into grove inside Tube

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Fill Line

• Insert Filter into tube … align arrow with notch at top of tube • Add more floatation fluid to fill line on Filter The OvaTube is now ready for centrifugation

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Carefully place OvaTube in centrifuge rotor – Fixed angle or swing-out – Fits most common centrifuges

Center of Rotation www.staspinvet.com

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Turn on centrifuge – 5 minute spin – 3000-3600 rpm (fixed angle) – 1200-1500 rpm (swing-out)

Center of Rotation

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Fecal material and debris sediment to bottom Parasite ova and cysts migrate to top Eliminates strainer, gauze and transfer steps!

Vegetation and “floaters� are trapped by Filter grid

Center of Rotation

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• When centrifuge stops, carefully remove OvaTube from rotor to work bench

Center of Rotation

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Place OvaTube in a secure rack, add coverslip to top of Filter

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Place OvaTube in a secure rack, add coverslip to top of Filter • Twist Filter clockwise to lift ova layer toward coverslip • Allow ova to settle for 3 minutes

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Place OvaTube in a secure rack, add coverslip to top of Filter • Twist Filter clockwise to lift ova layer toward coverslip

Standard Gravity flotation: centrifugal OvaTube: 20 flotation: minutes Only103minutes minutes!

U.S. and International Patents Pending

• Allow ova to settle for 3 minutes

In-clinic Ova and Parasite Detection

• Transfer coverslip to a microscope slide for examination

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Transfer coverslip to a microscope slide for examination

Slides are easier to scan because there’s far less debris with OvaTube

U.S. and International Patents Pending

… versus unreliable gravity flotation devices: • Better medicine! – – – –

2-3 times greater recovery1 Fewer false negatives “Best-practice technique” Urge customer to visit CAPCvet.org

1 - www.CAPCvet.org

… versus conventional centrifugal flotation: • Equal results with … – Less mess – Fewer steps – Less waiting

• Incremental cost is more than paid for in labor savings

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