Ova

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OvaTube: a bélsár legmodernebb centrifugációs felületdúsitásos vizsgálati módszere

Néhány fontos dolog… • 20-25 millió kutya és macska bélsárvizsgálatát végzik el évente az Egyesült Államokban • A kutyák 1/3-a gastrointestinális férgekkel fertözött www.petsandparasites.org

(CAPC)

• Évente 1-3 millió ember fertözödik meg Ancylostoma férgekkel, amelyet a kutyájuktól kapnak www.petsandparasites.org (CAPC)

• CAPC (Companion Animal Parasite Council) javaslata: 2-4 bélsárvizsgálat az elsö évben, aztán 1-2 évente

$

60 million+ pet dogs 70 million+ pet cats (AVMA Demographics 2008)

www.CAPCvet.org

Felszindúsitásos bélsárvizsgálat: egy fontos és kihasználatlan lehetöség!

200 million/year potential tests!

A kihasználatlan lehetöség… “Failure to use best-practice techniques, such as centrifugation, when conducting fecal flotation procedures can result in failure to detect parasite stages …” www.CAPCvet.org

• A tanulmányok azt mutatják, hogy a jelenleg használt passziv bélsár flotációs módszer (lásd a jobb alsó sarokban) elfogadhatatlanul magas false negativ eredményt ad • CAPC javaslata szerint kizárólag a centrifugációs felszindúsitásos módszer javasolt – a passziv flotációs módszer nem javasolt • Mivel több bélparazita zoonozisos betegséget okozhat, ennélfogva a pontos diagnózis rendkivül fontos mind az állat, mind a gazda számára.

The Opportunity … • A hagyományos centrifugációs módszer lassú, nagy munkabefektetést igényelt és kellemetlen feladat volt • Sok praxis emiatt nem végezte el a praxisban a bélsárvizsgálatot, vagy pedig egy nagy külsö laborba küldte be a bélsarakat, ezzel idöt, bevételt és azonnali kezelési lehetöséget vesztett Az OVATUBE módszerrel viszont a saját praxisodban végezheted el a centrifugációs felszindúsitásos bélsárvizsgálatot!

$8 to $12 per test

Introducing the …

Centrifugal Flotation Device for In-clinic Parasite Detection

• Centrifugal accuracy without the complexity and mess • Faster, far simpler method … diagnosis can be made while patient is in clinic • Better medicine for pets (and pet owners) … greater profits for the veterinarian An excellent recurring sales opportunity for you!

In-clinic Ova and Parasite Detection Mixer

Tube

Filter

SSOT-50 Dispenser box of 50 Kits

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Sample should be fresh ‌ < 1 hour or refrigerated

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Sample should be fresh ‌ < 1 hour or refrigerated • Press mixer into feces to collect 1 gram sample

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Sample should be fresh ‌ < 1 hour or refrigerated Long stem makes collection easier & more hygienic

U.S. and International Patents Pending

• Press mixer into feces to collect 1 gram sample

In-clinic Ova and Parasite Detection

• Add floatation fluid (e.g. zinc sulfate) to fill line on tube Common Floatation Fluids

Magnesium Sulfate

1.32

Sodium Chloride

1.20

Sodium Nitrate

Fill Line

U.S. and International Patents Pending

Specific Gravity

1.2-1.33

Sucrose

1.25

Zinc Sulfate

1.18

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with floatation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while “chopping” and twisting the Mixer • Remove the Mixer and discard

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Complete sample mixing and release of ova from fecal matter is achieved with far less effort and mess!

• Insert Mixer into tube with flotation fluid • Gently squeeze and release bulb while chopping and twisting the Mixer • Remove the Mixer and discard

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Insert Filter into tube ‌ align arrow with notch at top of tube

Ramp pin is inserted into grove inside Tube

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Fill Line

• Insert Filter into tube … align arrow with notch at top of tube • Add more floatation fluid to fill line on Filter The OvaTube is now ready for centrifugation

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Carefully place OvaTube in centrifuge rotor – Fixed angle or swing-out – Fits most common centrifuges

Center of Rotation www.staspinvet.com

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Turn on centrifuge – 5 minute spin – 3000-3600 rpm (fixed angle) – 1200-1500 rpm (swing-out)

Center of Rotation

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

Fecal material and debris sediment to bottom Parasite ova and cysts migrate to top Eliminates strainer, gauze and transfer steps!

Vegetation and “floaters� are trapped by Filter grid

Center of Rotation

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• When centrifuge stops, carefully remove OvaTube from rotor to work bench

Center of Rotation

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Place OvaTube in a secure rack, add coverslip to top of Filter

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Place OvaTube in a secure rack, add coverslip to top of Filter • Twist Filter clockwise to lift ova layer toward coverslip • Allow ova to settle for 3 minutes

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Place OvaTube in a secure rack, add coverslip to top of Filter • Twist Filter clockwise to lift ova layer toward coverslip

Standard Gravity flotation: centrifugal OvaTube: 20 flotation: minutes Only103minutes minutes!

U.S. and International Patents Pending

• Allow ova to settle for 3 minutes

In-clinic Ova and Parasite Detection

• Transfer coverslip to a microscope slide for examination

U.S. and International Patents Pending

In-clinic Ova and Parasite Detection

• Transfer coverslip to a microscope slide for examination

Slides are easier to scan because there’s far less debris with OvaTube

U.S. and International Patents Pending

Cost Comparison:

Send Out

… find out how the clinic is doing fecals now … then go for the sale:

OvaTube device

Gravity Std. OvaTube Flotation Method

Tongue depressor

• Far more accurate than gravity flotation devices

$0.13

Dixie cup

Flotation solution

• Far simpler and neater than standard method centrifugation • Far more cost effective than sending samples out

$0.02 $0.21

Gauze or strainer Gravity device

$1.29

$0.03 $0.50

Labor cost at $15/hour

$0.21

$0.21

$2.00

$3.75

$2.55

24 hours

8

15

10

Total Cost: $8-$12

$2.71

$4.14

$4.05

Total Time:

… versus unreliable gravity flotation devices: • Better medicine! – – – –

2-3 times greater recovery1 Fewer false negatives “Best-practice technique” Urge customer to visit CAPCvet.org

1 - www.CAPCvet.org

… versus unreliable gravity flotation devices:

“You already have all the equipment you need. Why not order a box of 50 and do some comparisons to see what you’ve been missing.”

… versus conventional centrifugal flotation: • Equal results with … – Less mess – Fewer steps – Less waiting

• Incremental cost is more than paid for in labor savings

… versus conventional centrifugal flotation:

“Why not order a box of 50 and see how OvaTube can improve your work flow and efficiency.”

… versus sending out to a commercial lab: • Lower cost … about $4/test (including labor) vs. $8-$12* • Better medicine … – – –

Less sample degradation Performed under the watchful eye of the veterinarian More timely results, often while the patient is in clinic

… versus sending out to a commercial lab:

“Why not order a box of 50 and see how OvaTube can enhance the value of your testing and add to your bottom line.”

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