Test Chemical Integrators In a 4-minute steam sterilizer cycle, natural kill happens at the 1.8minute check. Notwithstanding, AAMI suggests things that have conceivably interacted with traded off tissue be estimated past natural murder and up to a SAL of 10-6. Our chemical integrators change shading later in the sanitization cycle when each of the three basic parameters – time, temperature and immersed steam – are met, giving you included consolation that things were completely disinfected.