TITAN MEDIA AC BROTH TM 003 For cultivation of aerobes and sterility testing of biological products without mercurial preservatives Composition * Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C and protect from direct Sunlight. Instructions for Use Dissolve 34.20gms in 1000ml of distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Dispense the media in tubes. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. After storage, reheat the medium under hot water bath for few minutes with agitation. Cool without agitation. Appearance: Light to medium amber, clear to very slightly opalescent gel forms pH (at 25°C): 7.2 ± 0.2 Principle AC BROTH is used for cultivation of aerobes and sterility testing of biological products without mercurial preservatives. AC broth, a medium in which the cells do not produce enzyme and it is a good growth medium. Peptone, Beef extract and Malt extract provide nutrients and growth factors (carbon and nitrogen sources) required for a wide variety of organisms. Vitamins and cofactors required for growth as provided by Yeast extract. Dextrose is a carbon energy source. Ascorbic acid is added to clarify the solution. This media does not exhibit the toxicity shown by some media containing sodium thioglycollate for some microorganisms. Interpretation Cultural characteristics observed after inoculating (102 - 103CFU/ml), on incubation at 35 ± 2°C for 18 - 48 hours.
Microorganisms
ATCC
Inoculum (CFU/ml)
Growth
Clostridium perfringens*
12919
10²-10³
Good
Neisseria meningitidis
13090
10²-10³
Good
Staphylococcus aureus
25923
10²-10³
Good
Escherichia coli
25922
10²-10³
Good
*Incubated anaerobically References 1. Paper read at N. Y. meeting of American Public Health Association, (1944). 2. Malin and Finn, J. Bacteriology 62: 349. (1951). 3. American Type Culture Collection, Manassas, Va., U.S.A. For more details visit:- http://www.titanmedia.in/images/upload/td/TM%20003_ACBROTHTM003.pdf or http://www.titanmedia.in/