Nuliv Science Product Overview

PRODUCT LIST Clinically Validated Nutraceutical and Cosmeceutical Ingredients

nulivscience.com

ITEMS

BOTANICAL NAME

AVAILABLE GRADES

AbmPrime 40%

Agaricus blazei murrill

≥40% polysaccharide

AbmPrime powder

Agaricus blazei murrill

80 mesh

Acteolin™

Osmanthus fragans

≥20% acteoside

ActiGin®

Panax notoginseng, Rosa roxburghii

>30% saponine, >0.6% vitamin C, >2% polyphenols

AstraGin®

Astragalus membranaceus, Panax notoginseng

>1.5% total saponins

Astrion™

Astragalus membranaceus, Centella asiatica

≥0.25% total saponins, ≥0.4% Asiaticoside + Madecassicoside

ChagaPrime 25%

Inonotus obliquus

≥25% polysaccharide

ChagaPrime powder

Inonotus obliquus

80 mesh

CordycepsPrime™

Cordyceps sinensis

≥0.27% adenosine, ≥8% D-mannitol (Cordycepic acid)

CoriolusPrime 30%

Coriolus versicolor

≥30% polysaccharide

CoriolusPrime powder

Coriolus versicolor

80 mesh

HericiumPrime 20%

Hericium erinaceum

≥20% polysaccharide

HericiumPrime powder

Hericium erinaceum

80 mesh

InnoSlim®

Astragalus membranaceus, Panax notoginseng

≥2.5% total saponins

JiaogulanEx

Gynostemma pentaphyllum

≥80% gypenosides

MaitakePrime 25%

Grifola frondosa

≥25% polysaccharide

MaitakePrime powder

Grifola frondosa

80 mesh

NoCalSweet

Siraitia grosvenorii swingle

≥80% mogrosides

OsteoSine™

Proprietary blend of four herbs

≥2.0% flavonoids

PolyporusPrime powder

Polyporus umbellatus

80 mesh

ReishiPrime 30%

Ganoderma lucidum

≥30% polysaccharide

ReishiPrime powder

Ganoderma lucidum

80 mesh

RosaEx

Rosa roxburghii

≥6.0% vitamin C, 10.0% polyphenols

ShiitakePrime 30%

Lentinula edodes

≥30% polysaccharide

ShiitakePrime powder

Lentinula edodes

80 mesh

Verbasnol™

Rehmannia glutinosa

10% and 50% verbascoside

WulinshenPrime

Xylaria nigripes

≥0.078% adenosine, ≥7.5% polysaccharide

AB SO R AN PTIO TIO N B BO XIDA IOA VA NE N HE T AD ILA CA B IL A RD LTH APT OG ITY I EN CO OVA SC GN U I EY TIVE LAR EH HE E IM ALT ALTH H MU NE LU NG HEA L MO HEA TH OD LTH / SK ST RE IN S H SP EAL S TH OR T WE S NU IG TR H WE T M ITIO N A LL NE NAG SS EM EN T

PRIMARY INGREDIENTS

ASTRAGIN

®

In 16 in-vitro and eight in-vivo studies, AstraGin® has demonstrated to increase absorption and consequently final bioavailability of many essential and phytonutrients in the human body and repair damaged intestinal walls in TNBS-induced colitis rats. AstraGin® increases nutrient absorption by up-regulating the expression levels of absorption transporter mRNA and proteins, such as CAT1 and SGLT1, so a greater amount of the same nutrients can be absorbed into the body.

ENHANCES THE BIOAVAILABILITY OF PROTEIN, FATTY ACIDS, VITAMINS, & PHYTONUTRIENTS IN DIET THROUGH IMPROVED ABSORPTION THE CRITICAL ROLE ABSORPTION PLAYS IN BIOAVAILBILITY & HUMAN HEALTH Nutrients in foods and supplements must be digested and absorbed first before becoming available for vitalization.

Degree of digestion and absorption determines percentage of nutrients in foods and supplements that will become available in the blood stream. One’s ability to absorb nutrients can be impaired due to aging, stress, improper diet, medication, and sedentary lifestyle.

AstraGin ® reduces inflammation in colon bowel wall of colitis rats.

HOW ASTRAGIN WORKS IN THE HUMAN BODY

Repairs damaged intestinal wall in TNBS-induced colitis rats where most absorption occurs by decreasing MPO (a surrogate marker of inflammation) by ↓73% (in-vivo).

®

Ulceration Unclear surface Submucosal edema

The small intestine is the primary site for the absorption of fluids, electrolytes, macronutrients (proteins, carbohydrates, and fats), and micronutrients (vitamins, minerals, and trace elements). It selectively takes up nutrients from the intestinal lumen by modulating absorption transporter proteins (such as CAT1 and SGLT1) across the brush border membrane (BBM). The expression of nutrient transporter proteins at the BBM is dynamic and controlled by the availability of transporter proteins.

Normal colon cells

TNBS induced colon cells

Amnat Ruenroeng 2014-2016 IBF Flyweight Champion 2016 Thailand Olympic Boxing Team

Increases the absorption (in-vitro) of: •

Peptides and amino acids: ↑41% (AUC) (www.enxymessence.com)

• •

Arginine: ↑67%, Citrulline: ↑45%, Creatine: ↑33%, Agmatine: ↑36%

•

Beta-alanine: ↑26%, Tryptophan: ↑53%, Folate: ↑50%, Omega-7 fatty acid (Palmitoleic acid): ↑39% (AUC) in 30 min, Curcumin-lecithin: ↑92% in 2 hours

•

Glucosamine: ↑23% in 10 min

Increases the absorption (in-vivo) (TNBSinduced colitis rats) of: •

Lysine, arginine, and histidine: ↑38%, ↑31%, and ↑22%

•

Jejunum and ileum CAT1 protein: ↑25% and ↑151%

•

Jejunum and ileum mRNA: ↑22% and ↑8%

AstraGin ® -treated TNBS-induced colon cell

AFTER

BEFORE NUTRIENTS (AMINO ACIDS, FOLATE, ETC.)

BENEFITS

TRANSPORTERS

INTESTINAL LUMEN

EFFECT OF ASTRAGIN® ON ENHANCING NUTRIENTS ABSORPTION

NUTRIENTS (AMINO ACIDS, FOLATE, ETC.)

INTESTINAL LUMEN

TRANSPORTERS

US patent US8, 197, 860, applications US13/444, 765, US13/444, 770, US12/345, 218, US11426064

ASTRAGIN

®

A B C

IN-VITRO

A FURTHER LOOK BEYOND ABSORPTION — ASTRAGIN® AS A MULTIPLE METABOLIC PATHWAY ACTIVATOR All cells in the human body depend on a continuous supply of Adenosine triphosphate (ATP) to carry out their physiological and biochemical activities, such as the synthesis of transport protein for nutrient absorption. ATP level dictates the efficiency of energy-dependent processes and ATP-mediated signal transductions. Large amounts of ATP are used in muscle contraction, conduction of nerve impulse, compound biosynthesis and other biological processes.

AstraGin® has shown to increase mRNA, transporter protein, nutrient uptake, and ATP production in cells and animals (see diagram to the right). These studies and many reported effects on AstraGin® beyond absorption lead us to believe that AstraGin® may up-regulate multiple anabolic and catabolic metabolic pathways, such as glycolysis, glycogenolysis, lipid metabolism, mitochondrial metabolism, amino acids metabolism, gut microbiota metabolites, and functional components biosynthesis by optimizing the efficiency of tissues and cells to generate ATP from nutrients (fuel molecules). As such, AstraGin® may enhance many bodily functions through these metabolic pathways to promote overall health and general wellbeing.

IN-VIVO (TNBS-induced colitis rats after five days on AstraGin®)

IN-VIVO (Normal rats after one week on AstraGin®)

1 1 1

Activates mRNA Synthesis

Glucose: ↑105% Folate: ↑182% Cationic amino acids: ↑69%

Activates mRNA Synthesis

Ileum: ↑8% Jejunum: ↑22%

2 2

Activates Transport Protein Synthesis

Glucose: ↑87% Folate: ↑64%

Activates Transport Protein Synthesis

Ileum CAT1: ↑151% Jejunum CAT1 protein: ↑25%

3 3

Activates Absorption

4

Agmatine: ↑36% Arginine: ↑67% β-alanine: ↑26% Citrulline: 45% Creatine: ↑33% Curcumin-lecithin: ↑92% in 2 hours Folate: ↑50% Flax oil: ↑58% in 20 min Glucosamine: ↑23% in 10 min Leucine: ↑58% in 15 min Fish oil: ↑100% (AUC) in 20 min Omega-7 fatty acid: ↑39% (AUC) in 30 min Peptides and amino acids: ↑41% (AUC) (www.enxymessence.com) Tryptophan: ↑53%

Activates ATP Synthesis ATP: ↑18%

Activates Absorption

Arginine: ↑31% Histidine: ↑22% Lysine: ↑38%

Activates Absorption

Arginine: ↑16% Lysine: ↑16%

MECHANISM OF ACTION

THE CHALLENGE IN WEIGHT LOSS & METABOLIC SYNDROME MANAGEMENT Recent research has improved our understanding of several pathways in human body that influence weight regulation and disease risk. The endocannabinoid system has been found to regulate appetite and energy expenditure, as well as lipid and glucose metabolism. Blocking stimulation of this pathway to aid weight loss and reduce cardiometabolic risk factor development is an area of current interest and research.

INNOSLIM® AS A SOLUTION In a total of 16 in-vitro, 3 on Caco-2 cells, 3 on HSMMT (muscle) cells, 8 on 3T3-L1 (fat) cells, and 2 on HepG2 (liver) cells, and 2 in-vivo studies, 1 on OGTT (oral glucose tolerance test), and 1 on insulin, InnoSlim® has shown to regulate glucose, fat cells, and muscle cells metabolism and have positive effects in reducing glucose absorption in the gastrointestinal tract and in increasing fat cells combustion and muscle cells glycogen synthesis. Specifically, InnoSlim® decreases circulating glucose and reduces fat accumulation through the Adiponectin-AMPK-HIF-1-GLUT4 pathway and results in a favorable metabolic milieu for supporting type 2 diabetic conditions.

•

Decreases circulating glucose (in pre-clinicals) Supports healthy insulin sensitivity

60

*

40 20

AMPK ACTIVITY

FAT BURNING CELLS

350 250

200 150

100 50

0

GLUCOSE ABSORPTION

100 80 60 40 20

*

250 200 150 100

*

*

400 300 200 100

50 0

0

0

Decreases glucose absorption in intestinal cell (caco-2 cell) by 48%

300

120

300

HIF-1 (%)

Supports enhanced fat burning and fat loss

80

500

350

*

140

Phospho-ACC (%)

•

Supports healthy weight management

Phospho-AMPK (%)

•

160

100

0

Up-regulates the protein expression levels of phospho-AMPK, phospho-ACC, and HIF-1 in muscle cells

Phospho-ACC (%)

•

Relative Glucose Transport Rate (%)

STIMULANT-FREE WEIGHT & METABOLIC SYNDROME REGULATOR

BENEFITS

Phospho-AMPK (%)

INNOSLIM

®

350

*

300 250

200 150

100

Placebo 0.01 mg/Kg InnoSlim®

0.8 0.6 0.4 0.2 0.0

50

0

Up-regulates the protein expression levels of phospho-AMPK and phospho-ACC in fat cells

Time (min) Decreases insulin levels in Sprague-Dawley rats in 90 minutes by 38% (AUC) after oral glucose administration

InnoSlim®’s approach to weight loss and metabolic syndromes is novel and new in that it addresses the underlying metabolic derailment by correcting and optimizing glucose and fat metabolism.

US patent pending

A 1 INCREASES CALORIES BURNED

Increases Adiponectin

In brain and peripheral tissues to increase whole-body metabolic rates and burn fat as fuel

2

Increases AMPK

In muscle, liver, and fat tissue to increase fat burning in liver and skeletal muscle, to lower/reduce plasma glucose by suppressing the glucogenesis enzyme, and to increase glucose uptake in muscle and fat cells

3

INCREASES HIF-1

In muscle and liver to increase glucose delivered to muscle and maximize energy (ATP) production to burn more calories without increasing the oxidative level

In muscle and fat tissues

INCREASES GLUT4 to increase glucose uptake in muscle and fat cells

MECHANISM OF ACTION

B 1 DECREASES CALORIE INTAKE

Decreases Glucose Absorption

Decreases SGLT1 transporter in intestine to reduce glucose absorption

INCREASES PHOSPHO-ACC

2

Decreases Appetite

By decreasing glucose levels in the blood stream and increasing insulin sensitivity

In muscle and fat tissues to lower fatty acid synthesis

REDUCES INFLAMMATION & ENHANCES GLYCOGEN DELIVERY TO MUSCLE TISSUE FOR FASTER RECOVERY & SUSTAINED ENDURANCE

PRE-CLINICAL (in-vivo)

ActiGin® reduces muscle damage in exhaustive exercised rats

MDA (%)

ACTIGIN

®

200

Placebo

160

ActiGin®

120

*

*

80

BENEFITS ActiGin® reduces inflammation and consequently damage in muscles during high-intensity exercise: •

CK (creatine kinase) on day 4 after exercise by 69%

•

MDA (an oxidative stress marker) by 44% on day 4 after high intensity exercise

•

IL-6 (a pro-inflammatory cytokine) by 35% on day 4 after high intensity exercise

•

Increases energy catalyst citrate synthase activity by 47%

40 0 Non-exercise

THE CHALLENGE IN HIGH-INTENSITY EXERCISE AND PHYSICAL ACTIVITIES

ActiGin® has been proven to increase membrane fluidity of human cells, due to its hydrophobic advantage in partitioning into membrane lipid bilayer.

ActiGin® speeds up glycogen accumulation rate in muscles by 373% at the end of 3rd hour after a 60-min cycling exercise at 70% VO2max

Glycogen Accumulation Rate (mmol/kg/h)

ActiGin®, with its world-first proprietary Membrane Lipid Stabilization Technology, buffers the entropic stress against acute exercise challenge on sarcolemma (muscle cell membrane). This concept has been proven by four human clinical trials, which demonstrated a significant improved endurance performance (cycling time to exhaustion at 80% maximal oxygen consumption) with suppressed lipid peroxidation (TBARS), anti-inflammatory shift and significantly faster glycogen recovery after one hour exercise (70% maximal oxygen consumption) in doubleblind placebo controlled crossover trials. These promising results are detailed in the January issue of PLOS ONE.1

ActiGin® increases endurance in high-intensity exercise (80% VO2max) by 20% Endurance Performance (%)

ACTIGIN WITH THE PROPRIETARY MEMBRANE LIPID STABILIZATION TECHNOLOGY ®

HUMAN CLINICAL TRIALS

140 120 100 80 60 40 20 0

5 *

4 3 2 1 0 -1 -2 -3

ActiGin® reduces inflammation consequently damage in muscle at high-intensity exercise 100

Pecentage on Day 4 After Exercise (%)

Muscle contraction inevitably causes wear and tear of cell membranes leading to an accumulation of oxidized lipid components, which can undermine physical performance.

The intrinsic property of the membrane lipids represents a major limitation of human endurance performance since energy metabolism for ATP production requires normal cell membranes.

Exercise

Placebo

80 60 40

ActiGin® *

*

*

20 0

1: (https://www.ncbi.nlm.nih.gov/pubmed/25617625)

US patent pending

A

ENDURANCE PERFORMANCE

1 2 1 3

Increases Energy in Cells

Increases energy catalyst citrate synthase activity by 47% within 3h following a cycling exercise at 70% VO2max in a cross-over human clinical trial. Citrate synthase starts the energy production ATP (Krebs) cycle

Increases Glycogen in Muscles

B

MUSCLE FATIGUE RECOVERY

Speeds up glycogen accumulation rate in muscles by 375% at the end of the 3rd hour after a 60 min cycling exercise at 70% VO2max as determined by biopsy from quadriceps femoris muscle in a cross-over human clinical trial

Increases Glycogen in Muscles

3

Increases Time to Exhaustion

Increases time to exhaustion by 20% in a electrically braked cycle ergometer exercise at 80% VO2max in a cross-over human clinical trial

2 4

Reduces Lipid Peroxidation in Muscle Membrane

Speeds up glycogen accumulation rate in muscles by 375% at the end of the 3rd hour after a 60 min cycling exercise at 70% VO2max as determined by biopsy from quadriceps femoris muscle in a cross-over human clinical trial

Reduces lipid peroxidation marker TBARS by 24% at the end of the 3rd hour after a cycling exercise at 70% VO2max in a cross-over human clinical trial

Reduces Oxidative Stress Levels in Muscles

Reduces Physical Stress Levels in Muscles

In a cross-over human resistance exercise trial, level of oxidative stress in muscles was reduced as a result of lower MDA level in blood serum (by 44% on day 4)

MECHANISM OF ACTION

In a cross-over human resistance exercise trial, muscle damage due to lack of glycogen was decreased as a result of lower IL-6 level in blood serum (33% on day 4)

5

Reduces Damage in Muscles

In a cross-over human resistance exercise trial, damage in muscle fiber that leads to soreness and inflammation and restricts glycogen recovery was less as a result of lower creatine kinase (CK) in blood serum (69% on day 4)

ACTEOLIN

™

COGNITIVE, LUNG & EYE HEALTH

BENEFITS Pharmacological, in-vitro, in-vivo and clinical studies have demonstrated Acteolin™ is a multifunctional natural compound that: •

β-AMYLOID (Aβ) PLAQUES AND PHOSPHORYLATION OF TAU PROTEIN RESULT IN NEUROFIBRILLARY TANGLES THAT CAUSE NEURONAL LOSS IN CORTEX & HIPPOCAMPUS. BOTH LEAD TO ALZHEIMER’S DISEASE AND OTHER DEMENTIAS. Decline in Acetylcholine (ACh), the primary neurotransmitter in human brain, due to elevated Acetylcholinesterase (AChE) or decreased Choline Acetyltransferase (ChAT) in the brain leads to memory and learning impairment.

Improves cognitive and memory functions: • by reducing the accumulation of Amyloid beta peptide in the brain • by inhibiting abnormal phosphorylation

of tau protein to reduce neurofibrillary

tangles (NFTs) in the brain that cause

neuronal cell death • by protecting the central cholinergic system

•

Supports lung functions by ameliorating nuclear factor kappa β (NF-κβ) induced inflammation, by decreasing collagen accumulation in lung tissues by inhibiting transforming growth factor beta 1 (TGF-β1), and by inhibiting histamine release and tumor necrosis factor alpha (TNF-α) and interleukin 4 (IL-4) production

•

Extends life span by reducing free radical injury to cells

•

Protects retinal function by promoting the production of hepatocyte growth factor (HGF) to protect the retinal pigment epithelium (RPE) function

NF-κβ (nuclear factor kappa β) is a master switch of inflammation. Many studies have reported the role of NF-κβ in inflammation and proven the association of NF-κβ with human inflammatory lung diseases.

PULMONARY FIBROSIS OCCURS WHEN LUNG TISSUE BECOMES DAMAGED AND SCARRED Collagen accumulation is a major feature of emphysema and pulmonary fibrosis and other fibrotic lesions. Transforming growth factor beta 1 (TGF-β1) is a potent inductor of tissue collagen deposition.

Hepatocyte growth factor (HGF) regulates cell growth by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in tissue regeneration.

NuLiv Science employs a proprietary water extraction process (non-organic solvent) and computer-based satellite wheel double roller drying procedure to manufacture its proprietary acetoside, Acteolin™, from the Osmanthus fragrans plant that is native to Asia.

Acteolin™ has been validated to be an acteoside (C29H36O15)

by HPLC chromatography and UV spectra.

A B C D

IMPROVES COGNITIVE & MEMORY FUNCTIONS

SUPPORTS LUNG FUNCTIONS

PROMOTES LONGEVITY

SUPPORTS RETINAL FUNCTION

1 1 1 1

Reduces β-amyloid (Aβ) Plaques

Aβ plaques may lead to Alzheimer’s disease and other dimentias

Reduces Inflammation

Ameliorates kappa β (NF-κβ), a master switch of inflammation, induced inflammation

2 2

3 3

Reduces Neuronal Cell Death

Increases Nuerotransmitter in the Brain

Inhibits abnormal phosphorylation of tau protein to reduce neurofibrilary tangles in the brain that cause neuronal loss in the cortex and hippocampus

Decline in acetylcholine, the primary neurotransmitter in human brain, due to elevated Acetylcholinesterase (AChE) or decreased Choline Acteyltransferase (ChAT) in the brain leads to memory and learning impairment

Eliminates Lung Fibrosis

Inhibits Histamine Release

Decreases collagen accumulation in lung tissues by inhibiting transforming growth factor beta 1 (TGF-β1). Collagen accumulation is a major feature of emphysema and pulmonary fibrosis and other fibrotic lesions

Inhibits histamine release and tumor necrosis factor aplha (TNF-α) and interleukin 4 (IL-4) production

Extends Life Span

Extends life span by reducing free radical injury to cells

Protects Retinal Function

Protects retinal function by promoting the production of hepatocyte growth factor (HGF) to protect the retinal pigment epithelium (RPE) function

MECHANISM OF ACTION

Cordyceps sinensis is a parasitic mushroom found in the Tibetan high plateau. It has been used in China for thousands of years by royal and elite families as a tonic for energy, vitality, endurance, and for supporting general male wellness.

CordycepsPrime™ is derived from the mycelia of Cordyceps sinensis by a proprietary fermentation process. We use the original strain, verified by DNA fingerprinting.

In a manuscript published in Journal of Alternative and Complementary Medicine by Zhu JS et al.:

“Cordyceps sinensis shows its main activities in oxygenfree radical scavenging, anti-senescence, endocrine, hypolipidemic, anti-atherosclerotic, and sexual functionrestorative activities. The safety of the fungus, its effects on the nervous system, glucose metabolism, the respiratory, hepatic, cardiovascular, and immune systems, immunologic disease, inflammatory conditions, cancer, and diseases of the kidney have been conclusively studied in over 2,000 patients over a period of several decades.”

AUTHENTICATION OF SPECIES

BY PHARMACOGNOSY CordycepsPrime is identified and certified by colony, sporophore, conidiophore, and conidium characteristics of Cordyceps sinensis. The mycelia used in the production of NuLiv Science trademarked Cordyceps, CordycepsPrime™, was verified to be identical to Paecilomyces hepiali.

The tests were performed and reported by the Institute of Microbiology, the Chinese Academy Sciences Beijing, China on September 23, 1998.

The document number: (98) wei-jian 98130. The authentic copy of the certificate is in Chinese. ™

1

2

3

4

5

6

7

1, 2, 3, 8: other Cordyceps sinensis brands 4: Adenosine reference standard 5, 6, 7: Adenosine in 3 CordycepsPrime™ lots

0.15 0.10 0.05 0.00

2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00

Minutes

BY HPTLC & MICROSCOPY Through a 3rd party lab, Alkemist Labs, Costa Mesa, CA

Fig. 1 HPLC-UV profile for determining adenosine in CordycepsPrime™ (at 12.663 min)

4.00

WHY CORDYCEPS SINENSIS?

POTENCY

The only Cordyceps among the dozen popular Cordycep strains that has been conclusively studied in over 30 large human clinical trials is Cordyceps sinensis per The Scientific Rediscovery of an Ancient Chinese Herbal Medicine: Cordyeps sinensis, Part I and II.

CordycepsPrime™ has one of the highest natural biomarkers in “adenosine” and “D-mannitol” (cordycepic acid).

3.00 2.00 1.00 0.00 -1.00

SAFETY CordycepsPrime™ consistently passes all California Prop 65 heavy metal requirements. Prop 65 has the most stringent heavy metal requirements in the US.

8

12.663

CORDYCEPSPRIME ™

4.244

WHAT IS CORDYCEPS SINENSIS?

Improves oxygen utilization Supports robust lung function Reduces symptoms of fatigue Supports qualitative improvements in energy

AU

A PRIZED TRADITIONAL CHINESE MEDICINE MUSHROOM

• • • •

mV

CORDYCEPSPRIME

™

BENEFITS

0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50

Minutes Fig. 2 HPLC-ELSD profile for determining mannitol in CordycepsPrime™ (at 4.244 min)

A B C

INCREASES ENERGY LEVEL

IMPROVES OXYGEN UTILIZATION

SUPPORTS A MORE ROBUST LUNG FUNCTION

1 1 1

Elevates ATP:Pi Ratio

Increases energy level by elevating the ratio of ATP:Pi (adenosine triphosphate : inorganic phosphate)

Increases Oxygen Efficiency

Reduces oxygen consumption in a hypoxic environment for more efficient use of oxygen to support essential physiological activities of organs/tissues and greater tolerance to hypoxia-induced acidosis

Supports Lung Function

Increases intratracheal secretion to facilitate expectoration. Relaxes tracheal smooth muscle to reduce coughing

MECHANISM OF ACTION

OSTEOSINE

™

PROMOTES HEALTHY & STRONG BONE DENSITY

SEM (Scanning Electron Microscopy) FROM THE RA MODEL

THE BONE REMODELING PROCESS Bone is a dynamic tissue that is constantly being reshaped by osteoblasts, which produce and secrete matrix proteins and transport mineral into the bone matrix, and osteoclasts, which break down the tissue.

HOW OSTEOSINE ™ WORKS IN THE HUMAN BODY OsteoSine ehances the osteoblasts’ activity so more new bone tissues are built and decreases the osteoclasts’ activity so less old bone tissues are broken down. The net result of these two changes increases the bone density. OsteoSine™ enhances bone structural strength by increasing the width and surface area of trabecula, the “beams” that provide the structural support for the bone tissues, which is critical to bone strength.

Results of femoral head profile, bone trabecula and bone surface of normal, retinoic acid-induced osteoporotic and OsteoSine Complex™ treated rats.

BENEFITS •

Increases osteoblasts by 20%

•

Increases bone mineral density by 13-17% in PO rats

•

Increases bone mineral density by 9-14% in RA rats

•

Increases bone ash weight, calcium and phosphate content by 2.4 -7.4% in PO rats

•

Increases bone ash weight, calcium and phosphate content by 1.4 -18.8% in RA rats

•

Increases surface area and width of trabecula

•

Increases bone surface and skeletal structure

™

SUMMARY OF 15 SUBJECTS USING OSTEOSINE ™ FOR A YEAR OR MORE

PO (POST-OVARIECTOMIZED) MODEL OsteoSine™ helped ovariectomized rats recover from loss in bone mass caused by lack of female hormone. This model simulates menopause and post-menopause women.

RA (RETINOIC ACID-INDUCED ACUTE OSTEOPOROSIS) MODEL OsteoSine™ treated rats showed better bone matrix formation (SEM microscopy); increased lumbar spine and femoral bone mineral density; and increased calcium and phosphate contents of femur bone. It is suggested that these results are due to increased bone formation and decreased bone resorption activities.

A B

INCREASES BONE MINERAL DENSITY

INCREASES BONE STRUCTURAL STRENGTH

1 1

Increases New Bone Formation

Bone is a dynamic tissue that is constantly being reshaped by osteoblasts, which produce and secrete matrix proteins and transport minerals into the matrix, and osteoclasts, which breaks down tissue. OsteoSine™ increases the number of osteoblasts by 20% to increase the formation of new bone

2

Increases Bone Density

• Increases bone mineral density by 13-17% in post-ovariectomized rats • Increases bone mineral density by 9-14% in retinoic acid-induced rats • Increases bone ash weight, calcium and phosphate content by 2.4-7.4% in post-ovariectomized rats • Increases bone ash weight, calcium and phosphate content by 1.4-18.8% in retinoic acid-induced rats

Increases Width and Surface Area of Trabecula

OsteoSine™ increases the bone structural strength by increasing the width and surface area of trabecula, the "beams" that provide the structural support for the bone tissues, which is critical to bone strength

MECHANISM OF ACTION

VERBASNOL

50% verbascoside, lot #MRHTG20130722, from Nuliv Science

BEAUTY FROM WITHIN

mAU 1000

HPLC CHROMATOGRAPHY

BENEFITS

19.649

™

mAU 1750 1500

800

1250 1000 750 500

Verbascoside is a well-studied multipotent antioxidant for topical and oral applications. A highly effective cellular protectant with great potential as a nutritional supplement and for topical applications. Verbascoside (also known as acteoside), a highly studied phenylpropanoid, functions to protect the plants that contain this substance against physical, environmental and microbial harm.

Based on published papers, verbascoside reduces skin inflammation and potentially slows down hair loss by inhibiting the production of 5α-reductase and bacteria growth, and by decreasing pro-inflammatory chemokine IL-8. Verbascoside produces skin lightening effect by inhibiting the production of tyrosinase and promoting youthful skin by reducing the production of collagenase.

Verbasnol verbascoside is produced by NuLiv’s proprietary high purification extraction technology from Rehmannia glutinosa. Verbasnol™ is validated by HPLC chromatography and UV spectra to be the same verbascoside produced and marketed by leading verbascoside manufacturers. ™

400

19.297 20.662 23.707

VERBASCOSIDE: FOR CLEAR SKIN, UV PROTECTION & 5-α REDUCTASE INHIBITOR

200 0 05

10

15

20

25

28.591

600

30

200 0 35

min

200 250 300 350

•

Anti-Aging

•

Anti-Inflammatory

•

Anti-Oxidant

•

Collagenase Inhibitor

•

Sebum Regulating

•

Tyrosinase Inhibitor

nm

UV SPECTRA (125 ug/ml): water was used as solvent

ORAC (Oxygen Radical Absorbance Capacity) Value on Verbasnol™ (verbascoside) 10%

Results: Analysis

Result

Units

ORAC against hydroxyl radicals

3,223

μmole TE/gram

ORAC against peroxynitrite

278

μmole TE/gram

ORAC against super oxide anion

1,749

μmole TE/gram

ORAC against single oxygen

774

μmole TE/gram

ORAC 5.0 (sum of above)

7,630

μmole TE/gram

ORAC against peroxil radicals

1,606

μmole TE/gram

A B C

REDUCES SKIN IRRITATION & INFLAMMATION

ANTI-AGING

LIGHTENS SKIN

1 1 1

Inhibits Sebum Production

Inhibits the production of sebum regulating 5 Îą-reductase to reduce oily and waxy matters on the skin. Inhibits the production of pro-inflammatory chemokine IL-8 to reduce the inflammation in the skin. Inhibits bacteria growth on the skin

Decreases Collagen Breakdown and Oxidative Stress

Inhibits the production of collagenase that breaks down collagen in the skin and reduces oxidative stress with its high anti-oxidative activity

Decreases Pigment

Inhibits the production of Tyrosinase that produces melanin to darken the skin

MECHANISM OF ACTION

A PLANT-BASED SKIN REJUVENATING & HYDRATING PHYTOCOMPOUND WHAT IS ASTRION ™ & HOW IT WORKS IN THE HUMAN BODY

HUMAN CLINICAL

•

Reduces wrinkles and UV damage

•

Increases collagen and hyaluronic acid in skin

Astrion™ has demonstrated in 21 healthy individuals to reduce wrinkle volume by 15% and melanin by 17% in 4 weeks when compared to a control group.

•

Increases proline and glucosamine uptake

•

Astrion™ is NuLiv’s patented, clinically studied skin rejuvenating ingredient that can bring wrinkled, aged, damaged, dehydrated, and discolored skin back to life.

Astrion™ reduces the number and appearance of fine lines and wrinkles, and has shown in pre-clinicals to increase collagen synthesis in the dermal and epidermal skin layers.

Astrion™ is a proprietary blend of highly purified and fractionated extracts from Astragalus membranaceus and Centella asiatica produced by a proprietary pharmaceutical extraction and processing technology.

DAMAGED COLLAGEN

BENEFITS

PROLINE

GLUCOSAMINE

Topical and oral applications

UNIQUE DELIVERY SYSTEM Astrion™ can be delivered to epidermas, dermas, and subcutaneous layers through transcellular, transfolicular, stratum, and sweat ducts, and diffusion between cells because of its lipophilichydrophilic properties.

HYALURONIC ACID

HEALTHY COLLAGEN LOW LEVELS OF HYALURONIC ACID

AGING SKIN ION™ BEFORE ASTR

IN HEALTHY SK N™ IO AFTER ASTR

US Patent No. 7,959,952 TW Patent No. I362936 CN Patent No. 200710089586.3.

Before

Percentage (%)

ASTRION

™

100 80 60 40 20 0

PRE-CLINICAL TRIALS NuLiv in-vitro and human studies

After

A B C D

FIRMS

HYDRATES

REJUVENATES

MULTIPLE DELIVERY MECHANISM

1 1 1 1

Reduces Wrinkles

Reduces wrinkles by 15% in 21 healthy individuals in 4 weeks

Increases Hyaluronic Acid Synthesis Increases hyaluronic acid synthesis in HaCaT cells by 20%

Reduces Pigment

Reduces pigment by 17% in 21 healthy individuals in 4 weeks

2 2 2

Increases Collagen in HaCaT and HDF Cells • • • •

Collagen I synthesis by 80% and 60% Collagen III synthesis by 30% Collagen I secretion by 45% Collagen III secretion by 80%

3

Increases Proline Absorption Increases proline absorption in HaCaT cells by 27%

Increases Glucosamine Absorption Increases glucosamine absorption in HaCaT cells by 11%

Reduces UV Damage

Reduces MMP-1 synthesis in HDF cells by 50%

3

Increases Telomerase Activity

Increases telomerase activity in HaCaT cells by 365%

Unique Delivery Mechanism

Into epidermas, dermas, and subcutaneous layers through transcellular, transfollicular, stratum, sweat ducts, and diffusion between cells because of its lipophilic-hydrophilic properties

MECHANISM OF ACTION

PRIME BRAND MUSHROOM EXTRACTS & POWDERS TOXICITY

FUNCTIONS AGARICUS CORDYCEPS CORIOLUS

MAITAKE

REISHI

HERICIUM

CHAGA

SHIITAKE

MESIMA

POLYPORUS TREMELLA

XYLARIA

PRODUCT NAME

STUDY

Anti-inflammation Cardiovascular Immune Kidney Liver Mood Respiratory

Agaricus Blazei Cordyceps

Coriolus Maitake

Sleep Stress

Acute toxicity in mice; 25-day sub-chronic toxicity in mice 6-month chronic toxicity in rats and monkeys Genetic toxicity in mice

Genetic toxicity in rats and mice 6-month chronic toxicity in rats

Hericeum

Agaricus Blazei Murrill: Polysaccharide, Beta-glucan, RNA-protein Complex.

ChagaPrime (Inonotus obliguus): Polysaccharide, Beta-glucan, Befungin.

CordycepsPrime™ (Cordyceps sinensis): Adenosine, D-mannitol, Polysaccharide, Beta-glucan, Ergosterol, Nucleosides.

CoriolusPrime (Coriolus versicolor): PSK, PSP, Polysaccharide, Proteoglycan, Beta-glucan Protein Complex, Heteroglucan-peptide.

HericiumPrime (Hericium erinaceus): Polysaccharide, Beta-glucan, Hericenone A, B, C, D, E, F, G and H, Xylan, Heteroxylan, Heterogulcan, Proteoglycan.

MaitakePrime (Grifola frondosa): Polysaccharide, Beta-glucan.

MesimaPrime (Phellinus linteus): Polysaccharide, Monosaccharide, Glucuronic Acid, Peptide.

PolyporusPrime (Polyporus umbellatus): Polysaccharide, B-Vitamins: Thiamine, Riboflavin and Niacin.

ReishiPrime (Ganoderma lucidum): Polysaccharide, β-(1, 3)-D-glucan, Heteroglucan, Proteoglycan, Triterpenoids, Triterpenes, Nucleotides.

ShiitakePrime (Lentinus edodes): Beta-glucan, Heteroglucan, Adenine Derivative, Guanosine 5’ Monophosphate.

TremellaPrime (Tremella fuciformis): Polysaccharide, Glucuronoxylomannan, Heteoglycans, Mitomycin C. WulinshenPrime (Xylaria nigripes): Polysaccharide, Polysaccharopeptides.

Embryoxicity in rats; Teratogenicity in rats

Acute toxicity in mice Reishi Spore

Vitality

COMPOSITION

90-day sub-chronic toxicity in rats Acute toxicity in mice; Mutagenicity tests in mice

Genetic toxicity in rats; Antimutagenicity in rats

Shiitake

Acute toxicity in mice; Mutagenicity tests in rats and mice

Mesima

Acute toxicity in mice; Genetic toxicity in mice

Polyporus

Acute toxicity in mice; 28-day sub-chonic toxicity in mice

Tremella

Acute toxicity in mice; 6-month chronic toxicity in mice

SOLUBILITY, SMELL, AND TASTE PRODUCT NAME

SOLUBILITY

SMELL

TASTE

Agaricus Blazei

In water

Aromatic and slightly earthy

Slightly sweet and bitter

Agaricus Bisporus

In water

Aromatic

Plain

Coriolus

In water

Slightly aromatic

Slightly bitter

Hericeum

In hot water

Plain

Slightly bitter

Maitake

In hot water

Earthy

Plain and slightly sweet

In water

Plain

Plain

Polyporus Reishi Reishi Spore Shiitake

In hot water

Aromatic

Bitter and astringent

Slightly in water

Aromatic

Bitter

In hot water

Aromatic

Plain

NULIV SCIENCE AROUND THE WORLD

NULIV SCIENCE USA

A Perfect Blend of Science and Nature NuLiv Science is dedicated to the research, discovery, manufacturing, and marketing of patented novel evidenced-based nutraceutical & cosmeceutical ingredients through its well established translational research platform and advanced production technology.

In-vitro assay systems

In-vivo assay systems for efficacy and safety systems

Human use study

• •

Establish any cell assay system in 2 to 4 months Currently available cell assay systems: HaCaT and HDF cell, Caco-2, 3T3-L1, HSMMT,HepG2, HCH & SW 1353, etc.

• • • •

Nude mice for immune compromised db/db mice for diabetes and wound healing studies SD rat for ergogenic and diabetes studies absorption model (portal vein) Acute and sub-acute toxicity

• • •

Topical skin care test Ergogenic test Immune suppressed therapy

PHYTOMONITOR™ SYSTEM NuLiv PhytoMonitor™ quality control system provides strict quality control from raw materials to finished products.

NuLiv Science Offices

• • •

NuLiv Science Coverage

•

LEGEND

•

Assures accuracy of data on COA (Certificate of Analysis) issued by NuLiv Science Tests at manufacturing sites and NuLiv Science labs for double quality conformity NuLiv Science labs are equipped with state of the art equipments and staffed with qualified analytical professionals Tests and checks active markers, heavy metals, plate counts, physical characteristics, and if applicable, preservatives, pesticides, and herbicides Samples are tested by 3rd party laboratories annually

NOTES:

UNDERSTANDING THE CONCEPT OF QÌ: BRIDGING WESTERN SCIENCE & EASTERN TCM Qì, foreign in concept and practice in western medicine, is the root of traditional Chinese medicine (TCM). According to TCM, qì or ch’i, usually translated as “vital energy”, governs all aspects of biological and physiological activities in the human body. TCM practitioners have believed qì is used to measure the health, vitality and wellbeing of an individual.

Stronger qì is associated with better energy and physical stamina, stronger potential to ward off stress and other health ailments.

NuLiv Science’s 20 years of research in AstraGin® and its other proprietary ingredients, mostly comprised of qì herbs, such as Astragalus, leads us to believe the health benefits associated with qì herbs are related to the anabolic and catabolic metabolisms that regulate many basic biological functions in the human body that are manifested in physiological expressions in energy level and sense of wellbeing.

ABOUT NULIV HOLDING INC. NuLiv Holding Inc. is the parent company of NuLiv Science USA and NuLiv Wellness Clinic as well as a major shareholder in Steminent and DynaDx. NuLiv’s state of the art wellness clinic focuses on integrative services for anti-aging, treatment, and aftercare for cancer patients. Steminent focuses on adipose derived mesenchymal stem cells. Steminent has received US FDA Orphan Drug Designation for treatment of Spinocerebellar Ataxia. DynaDx uses complex systems analytics for health diagnostics. Together all four entities strive to expand the knowledge base and development of future health solutions.

NuLiv Science USA Steminent Biotherapeutics 1050 W. Central Ave. Bldg C Canada, China, Taiwan Brea, CA 92821 www.steminent.com Tel: (909) 594-3188 www.nulivscience.com sales@nulivusa.com DynaDx NuLiv Wellness Clinic USA, China, Taiwan Taipei, Taiwan www.dynadx.com www.nulivclinic.com NuLiv Research Labs Taipei, Taiwan

NuLiv QC & Production Labs Shanghai, China