Ijrtem 110103

Page 1

Invention Journal of Research Technology in Engineering & Management (IJRTEM) www.ijrtem.com ǁ Volume 1 ǁ Issue 10 ǁ

ISSN: 2455-3689

Fractionation of Crude Dye Extracted From Cucurbita Pepo Leaves by Cold Extraction Method Shradhanjali Mohapatra1 & Pragnyashree Mishra2 1

Assistant Professor, Department of Textile and Apparel designing, College of Home Science, OUAT. 2 Assistant Professor, Department of Floriculture and Landscaping, College of Horticulture, OUAT.

ABSTRACT: Natural dyes are those dyes obtained from natural sources. The majority of natural dyes are usually collected from roots, berries, bark, leaves, wood, fungi and lichens. Usually in ancient days people have dyed their textiles by using locally available materials. Cold extraction for crude dyes extraction from Cucurbita pepo leaves. Theextract obtained quantitatively from cold extraction method was 6.81g and 2.27g respectively from 100g and 50g of C. pepo dry mass taken in 750ml and 500ml of ethanol solvent.6 components/functional groups were confirmed in crude dye fractioned with n hexane, chloroform and ethyl acetate but only 4 components/functional groups were confirmed in crude dye fractioned with acetone.

INTRODUCTION Natural dyes are those dyes obtained from natural sources. The majority of naturaldyes are usually collected from roots, berries, bark, leaves, wood, fungi and lichens.Usually in ancient days people have dyed their textiles by using locally available materials. Woad, indigo, saffron, and madder were raised commercially and were important trade goods in the economics of Asia and Europe.A large number of investigations on extraction of colorants from natural sources like plants, microbes, insect and animals and their different kinds of applications have been done till date. In functional finishing oftextiles, coloration of food and dyesensitized solar cells natural dyehas already appliedShahid& Islam (2013). According toGrifoniet al. (2011)with naturaldye fabrics made of vegetable fibres has role in UV protection. Textiles offered a safestprotection from (UV) radiation exposer with the use of natural dyesincrease the UV protection of fabrics madeof vegetable fibre.A pumpkin is a gourd-like squash of thegenus Cucurbita belongs to the familyCucurbitaceaeCucurbita pepo or pumpkins are widely grown for commercial use and having good values in both food and recreation. This family consists of 125 generaand 960 species, mainly in tropicaland subtropical regions. Since long time efforts have been made by variousscientists to develop an efficient extractiontechnique for natural dye extraction.Baliarsinghet al.(2012) has isolated principal colour compounds such as flavonoids and tannin moietiesfrom two plant species (SaracaasocaandAlbizialebbeck), using solvent extraction method and standardised dyeing techniques, identified colourcomponents and checked their potentialantimicrobial properties against commonhuman pathogens. Hee-OckBooaet al (2012). extracted and characterized somenatural plant pigments and their use for cosmetics and determined antioxidantactivities, total polyphenols and flavonoids, and antimicrobial effects of some plant pigments.Natural pigment from the petals of the Flame of forest(Buteamonosperma) flower under differentoperating conditions such as extraction time,temperature and mass of the petals conventional extraction technique were extracted by by Sinha et al (2012). In the present study crude dyes are extracted from C. pepo leaves by cold extraction method. The fractionation of the dye was done with various solvents andanalysed by simple chromatographic technique.

MATERIAL AND METHODS This experiment was conducted at OUAT, Bhubaneswar All commercially available solvents such as were carried out. The curves were visualized under ultra violet at 254 nm in TLC. 2.1. Collection of C. pepo leaves The leaves of Cucurbita pepo was collected from nearby areas of OUAT in November 2016. 2.2. Preparation of sample The leaves of C. pepo were washed with tap water followed by distilled water toremove impurities/dust from the surface. The leaves were then dried in shade and powdered by blender. 2.3. Cold Extraction 100g of sample wassoaked in 500ml of ethanol solvent for twoweeks. 250 ml of ethanol was added to the soaked sample material in two batches for one week after removal of previous batch ethanol. Then all the layerswere separated and then mixed and filtered.In Rotary evaporator at reduced pressure, the ethanol filtrate wasevaporated to get dark green residue. Then Residue wasplaced in the water bath at the temperature 70oC for 4 hours. By this method ethanol content got evaporated and the ethanol extract which was collected in a vialand stored for fractionation. 2.4. Concentration of C. pepo leaves coldextract with rotary evaporator

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