Kaizen in Qc Microbiology – Kaizen Institute India

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SEPTEMBER 2013

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Project: POTABLE WATER SAMPLING FREQUENCY REDUCTION Production units :

Gemba :QCM Lab

Status : Before Kaizen

Location : campus Actions Taken During Kaizen

Evidence on the possible risk involved in 31 potable water sampling points tested for Total viable count and pathogens {number of tests-6] once in 15 days

reducing the testing frequency was gathered and recommendation of any control measure required on the risk identified for 31 potable water sampling points located at SFP, IFP, PFP and other facility was identified.

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Risk assessment being performed by assessing the mode of failure with control measures. Risk rating being evaluated by multiplying the risk level (severity) with available control measures. Based on the assessment sampling points was recommended for a sampling frequency of once a month Results & Benefits Summary Previous

After kaizen

Total sampling points analyzed = 31 points analyzed for every 15 days

Total sampling points analyzed = 31 points analyzed for every one month

Media Cost : for each analysis 160/- Rs. 160 * 31 (sampling points) = 4960/- * 2 (monthly twice) = 9920/-

4960/- per month Process Map Before & After Kaizen

Filter paper: For Each analysis 10/- Rs 10* 155(no. of filter papers for 31 sampling points) = 1550/- (for 15 days) * 2 = 3100/- per month

1550/- per month

Man Power : For each analysis 20minutes 20 * 31 (sampling points) = 620 minutes for 15 days 620 * 2 = 1240 minutes per month or 20.6 hr.

10.3 hours per month

HDPE Sampling Bottles : For each analysis 1 bottle per sampling point = 12/- per bottle 12 * 31 = 372/- Rs. For 15 days , 372*2 = 744/-

372/- per month

Total Cost = 1,65,168/Total Time = 247.2 hr. per year

Total Cost = 82,584/Total Time = 123.6 hr. per year

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Project :BMR REVISION Production units : NA

Location : campus

Gemba :QCM Lab

Status : Before Kaizen

BMR is lengthy and time consuming to fill them Repetitive entries Signatures required in many place Media preparation activity stage wise Sterilization chart pasted in BMR -duplication of autoclave log book

Results & Benefits Summary Cost savings for paper & printing – to be estimated Reduction in Autoclave cycle therefore savings of power Manual entries reduced, thereby reducing errors, time. Eg – Tacrolimus Seed inoculum development checklist will be reduced from 27 pages to about 16-17 pages

Actions Taken During Kaizen Park BMR’s revised Campus BMR’s - 16 numbers Reduction in number of pages, consolidation of raw material entry, media preparation activity for SF and IF combined, number of signatures reduced. Repetitive text removed Expected to be complete by 15/09/2013 Biological BMR’s -7 numbers Documentation time reduced by 60%; Preprinting of entries, number of signatures reduced

Process Map Before & After Kaizen Before

After

Lengthy BMR

Reduced pages

Media preparation separate for each stage

Combined media preparation for all stages

4 Reduced documentation


Project :CLOSED INOCULATION PROCEDURE FOR FERMENTERS Production units : SFP, IFP

Gemba :QCM Lab

Location : campus Actions Taken During Kaizen

Status : Before Kaizen

Small Molecules & Biologicals- Inoculation of fermenters was done using inoculation needle, which was essentially an open setup which required flaming before inoculating the fermenter

Introduction of closed system, comprising of male and female connectors which are connected in a sterile manner, no exposure to the surrounding environment Biological- Replaced the glass transfer flask with the Heavy duty vacuum bottle with sterile connectors since inoculum volume is less

Results & Benefits Summary

• Inoculum which is the basis for the start up for fermentation is not exposed to the external non clean environment • Removal of flame- safety aspect • Presence of sterile filter assures sterility

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Process Map Before & After Kaizen

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Project :REPLACEMENT OF COTTON PLUGS WITH SCREW CAPS Production units : NA

Gemba :QCM Lab

Status : Before Kaizen

Cotton plugs were used for all the flasks and bottles used

Cost for Cotton usage annually – 55508 INR

Results & Benefits Summary

• • •

Location : campus Actions Taken During Kaizen

Replaced the cotton with the screw cap bottles

Process Map Before & After Kaizen

Cost savings 1st year – 35252 INR Cost savings 2nd year onwards - ~ 1 lakh INR choking of the return air grills of the Biosafety cabinet with cotton fibers is avoided

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Project :FILTER INTEGRITY TESTING TRANSFERRED TO PRODUCTION Production units : SFP

Gemba :QCM Lab

Status : Before Kaizen

• • • •

QC Microbiology was testing integrity of filters used in the fermentation as well as downstream process in production Total Number of filters tested -450 in five months Documentation involved One analyst used to be completely deputed for this activity

Location : campus Actions Taken During Kaizen

Transferred the filter integrity testing to production since it does not involve any microbiology science Training of production personnel

Results & Benefits Summary Reduced one man power requirement which resulted in saving 1.98 Lakhs Documentation reduced

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Project :HARMONIZATION OF PCG SOP’s Production units : NA

Gemba :QCM Lab

Status : Before Kaizen

20 SOP’s for the MCB and WCB were being maintained Procedure was common but the documents were maintained strain wise

Location : campus Actions Taken During Kaizen

SOP’s reduced to 3 numbers Strain details entered in Annexure

Results & Benefits Summary

SOP preparation for new strains can be avoided Change controls reduced Maintenance of documents becomes simpler and easier to track

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Project :STOP IN USAGE OF FLAME IN BIOSAFETY CABINETS Production units : NA

Gemba :QCM Lab

Status : Before Kaizen

Used flame for all the aseptic activities handled in the BSC

Results & Benefits Summary

Safety compliance

No disturbance in the air flow, no turbulence caused because there will be no heat generated in the cabinet

Usage of gas cylinders reduced

Location : campus/PARK Actions Taken During Kaizen

Complete removal of usage of flame inside the BSC

Process Map Before & After Kaizen

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QC MICROBIOLOGY

Thank you

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