Probiotics referred to live bacteria, fungi, or yeasts that additive the gastrointestinal flora and support the maintenance of a healthy digestive system, thereby encouraging the growth performance and overall health of poultry. Probiotics are highly being included in poultry feed as substitutes to antibiotics. This systematic review helps a summary of the application of probiotics in poultry production and the potential role of probiotics in the nutrient application, growth and laying performance and gut health of poultry. Furthermore, the poultry industry has become an important economic activity in various economies.