Dr. ADHIKARLA SYAMA Assistant Professor Bepartment of Biotech, FET MRIIRS, Faridabad e-mail: syama.fet@mriu.edu.in TEACHING EXPERIENCE 1.
Assistant Professor at Dept. of Biotech, FET, Manav Rachna International Institute of Research and Studies, Faridabad August 2017 – till date I have been teaching undergraduate and postgraduate students of Biotechnology the subjects viz.,Biochemistry, Animal biotechnology, Food packaging technology, Thermodynamics of a bioprocess, biosensors and biochips, DNA microarray and its applications. I also take practical classes for the subject Bio analytical techniques and biochemical engineering for undergraduate students.
RESEARCH INTERESTS Evolutionary and disease genomics. RESEARCH EXPERIENCE 1.
Institute of Genomics and Integrative Biology, Delhi Jan 2017 – Aug 2017 Worked as Research Associate I at IGIB in GOMED project. This project mainly focussed on the bioinformatic analysis of omic data of patients with rare genetic disorder and assist medical professionals in their diagnosis and
treatment. My
role was to analyse the data, mainly whole exome/genome and provide genetic summary to medical professionals. 2.
Institute of stem cell biology and regenerative medicine April 2015 - August 2016 During my doctoral research period, I worked on modelling neuropsychiatric disorders and understanding the mechanism of these diseases by bridging the genotype -phenotype gap using NGS data sets and pipelines
3.
Research Associate at National Institute of Mental Health and Neurosciences, Bangalore Nov 2013 – Mar 2015 Studied the effects if inbreeding in a cohort of Schizophrenia cases in a rural south Indian population. The study focussed on inbreeding effects on the clinical, socio-demographic and minor physical anomalies as endophenotype variables.
4.
Post Doctoral Fellow at Chettinad Academy of Research and Education, Chennai Worked for Genographic India Project sponsored by National Geogaphic Society, IBM and Waitt fmily foundation. The primary aim of this project was to identity the genetic basis of human migration using Non-Recombining Y chromosome and Mitochondrial DNA in Indian population.
TECHNIQUES KNOWN
NGS data analysis Whole exome sequencing &Whole genome sequencing- data extraction, QC and data mining RNA sequencing- data extraction and analysis Column chromatography
Genomic techniques and analysis Familiar with techniques of DNA extraction: Human DNA, plant and bacteria. DNA quantification and automated DNA sequencing
Polymerase chain reaction SNP genotyping using the technique of real time PCR YSTR and indel genotyping using PCR techniques in 3130xl genetic analyzer (16 capillary sequencer) Mitochondrial DNA sequencing by big dye terminator technique Electrophoresis techniques like agarose gel electrophoresis Experience in field work for collecting mouthwash samples (Collected mouthwash samples from Andhra Pradesh, Karnataka, Tamil Nadu and Maharashtra states as a part of Genographic project and thesis) Can perform statistical analysis using software’s like Arlequin, Mega, Network, R-statistical package, BATWING, Spagedi, PHYLIP, STRUCTURE and SPSS.
EDUCATION Doctor of Philosophy Madurai Kamaraj University, Madurai, Tamil Nadu Thesis title: Studies on the migration and isolation of selected ancient populations of
India-
a non recombinant Y chromosome (NRY study) Guide:
Dr. RM. Pitchappan (Professor (Retd), SBS, MKU)
Master of Science, Biochemistry University of Bangalore Bachelor of Science, Chemistry, Botany, Zoology University of Bangalore
HONORS / AWARDS 1. Postdoctoral research fellowship, Institute for Stem cell biology and regenerative medicine (INSTEM), Bangalore. 2. Post doctoral fellowship from Chettinad academy of research and education (CARE), Chennai. 3. Junior Research Fellow with the Genographic Project situated in the School of Biology, Madurai Kamaraj University sponsored by the National Geographic Society, Washington D.C. 4.Selected for Summer Research Fellowship Programme on All India basis by Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore and worked on a project titled on “In- vitro micro-propagation of Mentha citrata” under the guidance of Dr D H Tejavathi, Dept of Botany, Bangalore University. 6. Certificate of merit by Christ College, Bangalore for securing III place in I CBZ examination conducted during the year 2001-2002 7. Certificate of merit by Christ College, Bangalore for securing III place in ICBZ examination conducted during the year 2003-2004
PUBLICATIONS
Genetic testing for clinically suspected spinocerebellar ataxia: Report from a tertiary referral centre in India.
Sowmya Devatha Venkatesh, Mahesh Kandasamy, Radhika Vaidyanathan, Nagaraj S Kota, Syama Adhikarla, Ravi Yadav, Pramod Kumar Pal, Journal of Genetics. Volume 97, Issue 1, pp
Moily, LakshmiNarayan
Sanjeev Jain, Meera Purushottam. 2018.
219-224.
Mutation burden profile in familial Alzheimer's disease case from India.
Adhikarla Syama, Somdatta Sen, Lakshmi Narayan Kota, Biju Vishwanath, Meera Purushottam, Mathew Varghese, Sanjeev Jain, Mitradas M Panicker and Odity Mukherjee. 2017, Neurobiology of ageing. Volume 64, pp 158e7-158.e13.
Adverse Effects of Culture Media on Human Pluripotent Stem Cells. MeghaPrakash Bangalore, Odity Mukherjee, Syama Adhikarla, Mitradas M.Panicker. 2017, Scientific reports. NPG. 2017; 7: 42222. Published online 2017
Feb 8. doi: 10.1038/srep42222.
Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured
for
Therapeutic
Applications.
,
Behnam Ahmadian Baghbaderani Adhikarla Syama (equal first author)., Renuka Sivapatham, Ying Pei, Odity M, Thomas Fellner, Xianmin Zeng, Mahendra S. Rao.
2016 Stem Cell Rev.
Aug;12(4):394-
420. doi: 10.1007/s12015-016-9662-8.
A late Neolithic expansion of Y chromosomal haplogroup O2a1‐M95 from east to
GaneshPrasad ArunKumar, Lan Hai, Valampuri John Kavitha, Adhikarla Syama, Varatharajan Santhakumari
west.
Arun, PK Nyak, PK Das, Premanada Panda, Sreekanth Patel, Hui Li, Ramasamy Pitchappan. 2015 Journal of systematics and evolution. doi: 10.1111/jse.1214 Genome-wide signatures of male-mediated migration shaping the Indian gene pool. ArunKumar, GaneshPrasad, Tatiana V. Tatarinova, Jeff Duty, Debra Rollo, Adhikarla Syama, Varatharajan Santhakumari Arun, Valampuri John Kavitha et al. 2015, Journal of human genetics. J Hum Genet. 2015 Sep;60(9):493-9.
Neolithic mitochondrial haplogroup H genomes and the genetic origins of Europeans.Brotherton, Paul; Haak, Wolfgang; Templeton, Adhikarla Syama; Ganesh Prasad, Arun Kumar; Pitchappan, Ramasamy; Varatharajan Santhakumari, Arun; Soria Hernanz, David F; Spencer Wells, R; Swamikrishnan, Pandikumar;Tylersmith, Chris;Paulo Vieira, Pedro; Ziegle
Janet S et al., 2013.
Nature communications (1764).
doi:10.1038/ncomms2656.
Male lineages from Tamil Nadu, South India, reveal a local expansion and population differentiation predating the establishment of the caste system. Ganesh Prasasd Arunkumar, Valmapuri John Kavitha, David F Soria-Hwernanz, Varatharajan Santhakumari Arun, Adhikarla Syama, Kumarasamy Ashokan, Kavandapatti Thangaraj Gandhirajan, Koothapuli Vijaykumar, Muthusamy Narayan, Mariakuttikan JayaLakshmi, Janet S Zeigel, Ajay K Royaru, Laxmi Parida, R Spencer Wells, Colin Renfrew, Theodre G Schurr, Chris Tyler Smith, Daniel Platt, Ramasamy
Pitchappan
and
Genographic
Consortium.
2012.
PLoS
ONE
7(11):e50269.
doi:10.1371/journal.pone.0050269.
Partial Purification and Chemical characterization of Extracellular Pectinase from Aspergillus niger isolated from groundnut seeds. Sneha Gopal, Syama A, Shabana Sultana, Radhika Shreedhar, Reshmi Ragunath and Narayana Murthy. 2008. Journal of Applied Biosciences. Vol.9 No.1 pp.378-384 ref.35
Plantlet regeneration from callus and stem node explants of Mentha citrata, Tejavathi D H, Raveesha H R, Sowmya R, Maruthi Kumar D R, Gayathramma K, Pushapavathi B, Shyama A, Balasubramanyam. 2007. J med
aromatic Pl Sci, 29(1), 32.
Preliminary study of faunal diversity in Madiwala Lake.
Syama A, P U Antony, 2003, Mapana, Vol.2, No.1 pp 52-57. The above study was presented to Indian Institute of Science, Bangalore at national level, 2003. This paper was adjudged as the best.
Book Chapter- Genomic perspectives of peopling and languages of the Indian sub continent”.Genomics and Health in the Developing world
GaneshPrasasd Arunkumar, Varatharajan Santhakumari Arun, Adhikarla Syama, Valmapuri John Selvam Kavitha and Ramasamy M Pitchappan. Edited by Dhavendra Kumar
PRESENTATIONS 1. Modelling neuropsychiatric disorders using patient derived cell lines a workflow for bridging genotype-phenotype gap at Instem annual talks held on March 7th, 8th and 9th 2016 at INSTEM, Bangalore. 2. Human induced pluripotent stem cells based modelling of late onset Alzheimer's disease (LOAD) genetics in an Indian cohort at NCBS annual talks held on January 7th-9th 2016 at NCBS, Bangalore 3. Metabolic implications of retinol in Human pluripotent stem cells at NCBS annual talks held on January 7th-9th 2016 at NCBS, Bangalore th 4. Participated as a teacher in “Open house” held on 15 February 2013 at Chettinad academy of research and education, Chennai. 5. Participated in the annual Genographic meeting at Washington DC in October 2011 6. Presented a poster on the “Mouth wash technique” of obtaining human DNA in Chettinad Health Centre, Chennai in March 2011 7. Presented a poster on “NRY genetic signatures of Andhra Pradesh” in March, 2011 for Annual Science Day, Aqua Terr, Madurai Kamaraj University. 8. Presented a poster in Genomic techniques of Genographic on February, 2011 at Kaleeshwari College, Sivakasi 9. Oral presentation during national seminar on conservation of biodiversity in protected areas- opportunities and challenges, Christ University, Bangalore in January, 2009 10. Oral presentation on partial purification and chemical characterization of extracellular pectinase from Aspergillus niger isolated from groundnut seeds. National seminar on fungal biodiversity and bioinformatics. 32nd annual meeting of the mycological society of India, Mahavir Jain College, Bangalore, February 2006. MANAGERIAL SKILLS & EXTRACURRICULAR ACTIVITIES
Coordinating Industrial training for B.Tech students Student Mentoring Training B. Tech students for their ITR projects Trained in Indian Classical dance: Bharatanatyam and Kuchipudi Trained in various types of paintings from VAC academy of arts and crafts, Madurai