DEVELOP TOOLS AND DEVICES TO INTERFACE BIOMATERIAL-TOELECTRONIC Presented at:
Kentucky Innovation and Entrepreneurship Conference Aug 2013
By
Mehdi M. Yazdanpanah, PhD CEO-Founder, NaugaNeedles LLC
NaugaNeedles History Established in 2007 by name and become operational in 2009 Licensed UofL technology and filed for 4 utility patents Established its fabrication facility in Louisville KY Currently have more than 200 customers world wide Have 8 employees (4 full time and 4 part time) Supported by SBIR grants from NSF and NIH Supported by State and local program (e.g. SBIR matching fund, DCI forgivable loan, Vogt award)
Outline NaugaNeedles platform technology Our current product and tools Current R&D toward future product development
Formation of Ag2Ga Needles in Ambient Condition
Ag-coated substrate
ďƒ˜A gallium line on top of silver ďƒ˜Highly crystalline Ag2Ga nanowires are spontaneously self-assembled in few seconds inside the gallium line
10 mm
SELECTIVE GROWTH IN AMBIENT CONDITION
Ga droplet
10 mm
Ag2Ga nano-needle 120 nm in diameter and 70 µm long
Growing from a melted drop of gallium at room temperature
Nanoneedles Batch Fabrication 200 mm
(a) Gallium substrate
Array of silver coated tips Tips are not visible at this magnification
(b) 30 mm (c)
(d)
10 mm
(e)
PROPERTIES OF NANONEEDLES IN A GLANCE Mechanical Ultra sensitive cantilever (k < 0.1 mN/m, f0 > 100 KHz) E = 84 ± 1 Gpa
Electrical Highly conductive: comparable to platinum and copper High current capability: 5 mA Thermal: Melting point: Experimentally measured: 950 ºC, • Long term stability in air
•
A thin layer of oxide forms around the nanowire and protect the rest
Highly resistant to Reactive Ion Etching
Example of NaugaNeedles Products
5 mm
Customers: Researchers ( e.g. material scientist, biologist, chemist, pharmaceutical industry) 8 types of products and more than 100 part number is currently offered More details at http://nauganeedles.com/products.html
CURRENT R&D TOWARD FUTURE PRODUCT DEVELOPMENT
Metrology tools for MEMS and semiconductor industry Device for Capturing Circulating Tumor Cell in Human Blood
GAP IN METROLOGY TOOLS IN MICROSTRUCTURES IMAGING
Gap in Metrology Tools
NANOTOUCH FOR SIDEWALL SCANNING AND DEPTH MEASUREMENTS AT MICROSCALE (a)
(b)
Preamplifier board
Vibrated Needle in action
spring loaded kinematic mount Alumina Chip
Tuning fork Nanoneedle
NaugaNeedles in collaboration with InSituTec is developing the NanoTouch to address the gap in metrology
Results of Sidewall Imaging Using NanoTouch
Depth Measurement Using NanoTouch
A Handheld detector to detect and capture low dose Circulating Tumor Cell (CTC) in 1 ml of blood
PROBLEM REGARDING DETECTION AND CAPTURING OF LOW DOSE CTC Circulating tumor cells (CTC) are cells that detach from primary tumors and circulate in the blood stream Early detection of low counts of CTCs in patient blood sample can be used as a valuable indicator for diagnosis How can 1 to 10 CTCs mixed with 5 billion Red and White blood cells be detected?
EXAMPLE OF A MICROCHIP CTC CAPTURING DEVICE
Capturing method: micropillar array
Detection Method: florescence microscopy
EXAMPLE OF A MICRO FLUIDIC DEVICE FOR CTC CAPTURING
Capturing method: microstructure array Detection Method: florescence microscopy http://www.msnbc.msn.com/id/40881967/ns/health-cancer/
THE PROPOSED MECHANISM FOR CTC CAPTURING AND COUNTING A
WBC CTC
RBC
SELECTIVE CAPTURING MECHANISM USING METALLIC NANONEEDLES ARRAY
PRELIMINARY RESULT OF CAPTURING INDIVIDUAL CTC Biotinylated Antibody
A CTC from a MCF-7 cell line stuck to a nanoneedle in the array
Neutravidin GMBS Silane
10 µm
In Summary Briefly introduced NaugaNeedles history and the platform technology Future device development for depth and sidewall imaging for MEMS and semiconductor industry Preliminary results for developing a blood test kit for capturing and counting circulating tumor cells
Sponsors