2013 KIEC - Directed Self-Assembly Of Nanostructures To Develop Tools And Devices To Interface...

DEVELOP TOOLS AND DEVICES TO INTERFACE BIOMATERIAL-TOELECTRONIC Presented at:

Kentucky Innovation and Entrepreneurship Conference Aug 2013

By

Mehdi M. Yazdanpanah, PhD CEO-Founder, NaugaNeedles LLC

NaugaNeedles History  Established in 2007 by name and become operational in 2009  Licensed UofL technology and filed for 4 utility patents  Established its fabrication facility in Louisville KY  Currently have more than 200 customers world wide  Have 8 employees (4 full time and 4 part time)  Supported by SBIR grants from NSF and NIH  Supported by State and local program (e.g. SBIR matching fund, DCI forgivable loan, Vogt award)

Outline NaugaNeedles platform technology Our current product and tools Current R&D toward future product development

Formation of Ag2Ga Needles in Ambient Condition

Ag-coated substrate

ďƒ˜A gallium line on top of silver ďƒ˜Highly crystalline Ag2Ga nanowires are spontaneously self-assembled in few seconds inside the gallium line

10 mm

SELECTIVE GROWTH IN AMBIENT CONDITION

Ga droplet

10 mm

 Ag2Ga nano-needle 120 nm in diameter and 70 µm long

 Growing from a melted drop of gallium at room temperature

Nanoneedles Batch Fabrication 200 mm

(a) Gallium substrate

Array of silver coated tips Tips are not visible at this magnification

(b) 30 mm (c)

(d)

10 mm

(e)

PROPERTIES OF NANONEEDLES IN A GLANCE Mechanical  Ultra sensitive cantilever  (k < 0.1 mN/m, f0 > 100 KHz)  E = 84 ± 1 Gpa

Electrical Highly conductive:  comparable to platinum and copper High current capability: 5 mA Thermal:  Melting point: Experimentally measured: 950 ºC, • Long term stability in air

•

A thin layer of oxide forms around the nanowire and protect the rest

 Highly resistant to Reactive Ion Etching

Example of NaugaNeedles Products

5 mm

Customers: Researchers ( e.g. material scientist, biologist, chemist, pharmaceutical industry) 8 types of products and more than 100 part number is currently offered More details at http://nauganeedles.com/products.html

CURRENT R&D TOWARD FUTURE PRODUCT DEVELOPMENT

Metrology tools for MEMS and semiconductor industry Device for Capturing Circulating Tumor Cell in Human Blood

GAP IN METROLOGY TOOLS IN MICROSTRUCTURES IMAGING

Gap in Metrology Tools

NANOTOUCH FOR SIDEWALL SCANNING AND DEPTH MEASUREMENTS AT MICROSCALE (a)

(b)

Preamplifier board

Vibrated Needle in action

spring loaded kinematic mount Alumina Chip

Tuning fork Nanoneedle

NaugaNeedles in collaboration with InSituTec is developing the NanoTouch to address the gap in metrology

Results of Sidewall Imaging Using NanoTouch

Depth Measurement Using NanoTouch

A Handheld detector to detect and capture low dose Circulating Tumor Cell (CTC) in 1 ml of blood

PROBLEM REGARDING DETECTION AND CAPTURING OF LOW DOSE CTC Circulating tumor cells (CTC) are cells that detach from primary tumors and circulate in the blood stream Early detection of low counts of CTCs in patient blood sample can be used as a valuable indicator for diagnosis How can 1 to 10 CTCs mixed with 5 billion Red and White blood cells be detected?

EXAMPLE OF A MICROCHIP CTC CAPTURING DEVICE

Capturing method: micropillar array

Detection Method: florescence microscopy

EXAMPLE OF A MICRO FLUIDIC DEVICE FOR CTC CAPTURING

Capturing method: microstructure array Detection Method: florescence microscopy http://www.msnbc.msn.com/id/40881967/ns/health-cancer/

THE PROPOSED MECHANISM FOR CTC CAPTURING AND COUNTING A

WBC CTC

RBC

SELECTIVE CAPTURING MECHANISM USING METALLIC NANONEEDLES ARRAY

PRELIMINARY RESULT OF CAPTURING INDIVIDUAL CTC Biotinylated Antibody

A CTC from a MCF-7 cell line stuck to a nanoneedle in the array

Neutravidin GMBS Silane

10 µm

In Summary  Briefly introduced NaugaNeedles history and the platform technology Future device development for depth and sidewall imaging for MEMS and semiconductor industry Preliminary results for developing a blood test kit for capturing and counting circulating tumor cells

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