Tanner Scientific Semi- Automatic Cyostat - TN9000 User Manual

User Manual

TN9000

CRYOSTAT

Semi-Automatic Cryostat

Read Prior to Operation!

Always Keep This Manual Near the Unit!

57515 07.21.16

Information The information, notes, and figures that appear in this user manual represent the present state of knowledge as comprehended. Due to continuous improvements in technology and manufacturing techniques, specific and manufacturing procedures may change.

For inaccurate statements, drawings, and technical illustrations in this user manual, we exclude liability as far as permissible. No liability is accepted for any monetary loss or consequential damage caused by or related to compliance with statements or other information in this user manual. Tanner Scientific ÂŽ will update the manual as needed according to the latest technological developments.

This document is protected under copyright laws. Any reproduction of illustrations or text requires prompt documented consent by Tanner Scientific ÂŽ.

Statements, drawings, illustrations and other information regarding content or technical details of the present user manual are not to be considered as warranted characteristics of our products. These are determined only by the contract provisions agreed between us and our customers.

Refer to the name plate on the back of the unit for the serial number and manufacturing date of this instrument.

Manufactured by Tanner Scientific 1107 Tallevast Rd Sarasota, Florida 34243 888.708.5233 Info@TannerScientific.com www.TannerScientific.com

Contents 1 | General Summary...................................................................................................4 2 | Safety Precautions................................................................................................5 3 | Technical Parameters.........................................................................................6 4 | Installation......................................................................................................7 5 | Operation...........................................................................................................8 5.1 | Blade Holder.....................................................................................................................8 5.2 | Specimen Disc Installation.................................................................................11 5.3 | Freezing Specimen...................................................................................................12 5.4 | Defrost.....................................................................................................................13 5.5 | Vertical View.................................................................................................................14 5.6 | Touch Screen Display............................................................................................14 5.7 | Auxiliary Function Setting.................................................................................17 5.8 | Time Setting.................................................................................................................18 5.9 | Course Feed Control Panel...............................................................................20 5.10 | Maintenance...........................................................................................................22

6 | Cleaning & Maintenance..............................................................................23 7 | Warranty & Service............................................................................................25 8 | Troubleshooting...........................................................................................26

Tanner Scientific ® TN9000 • Semi-Automatic Cryostat 1

1 | General Summary The TN9000 Semi-Automatic Cryostat Microtome is a medical device used for rapid freezing of pathological sections of human and animal tissue. It can be widely used for pathological diagnosis, analysis and research in hospitals, medical colleges, and by legal medical experts and institutes.

Instrument Consists of 4 Major Parts: • The touch screen display is located on the upper part of the instrument.

This area displays the general working conditions and the temperature of the instrument.

• The chamber area is used to rapidly freeze the tissue and to conduct cutting. The microtome assembly is located inside the chamber area.

• The compressor, cooling condenser and evaporator are located in the lower part of the instrument.

• Behind the chamber is the mechanical transmission and motor drive.

Touch Screen Display

Control Panel

Power Switch

Glass Window

Hand Wheel

Liquid Waste Bottle

Stabilizing Feet

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2 | Safety Precautions This user manual includes important instructions and data related to the operating safety and maintenance of the instrument. The user manual is an important part of the product. It should be read carefully before using the instrument for the first time and must always be kept with the instrument.

This cryostat may only be operated by qualified personnel. In case of malfunction, contact a qualified service technician. Never try and solve the problem yourself.

Power Supply • A professional electrical engineer should inspect the electrical circuit before installation to ensure smooth operation of the instrument.

• The instrument uses a single phase AC power supply: the voltage is 110V±10%. If the power does not meet this requirement, then a 2000-3000VA AC voltage regulator shall be connected for usage.

• A dedicated 20AMP circuit is required.

• Confirm the power supply is well grounded (triple socket) before connecting the instrument with the main power socket. Turn on the power switch in the back of the instrument to start the machine.

Temperature • The cryostat’s working (environment) temperature is 5°C ~ 23°C (40°F ~ 73°F).

High temperature or humidity will impact the chamber temperature and shorten compressor life.

Operating • Exercise extreme caution when handling blades. Always remove when cleaning the inside of the chamber. Never place a blade face up as the sharp surface can lead to serious injury.

• NEVER TRY TO CATCH A FALLING BLADE! • Before changing specimens, always lock the hand wheel and cover the blade edge with the blade guard. Make sure the hand wheel is in the locked position when the instrument is not in use.

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3 | Technical Parameters Section Thickness Range: 1-90µm Adjustable,

1-20μm, 1μm increments

20-40µm, 2µm increments

40-90µm, 5µm increments Trimming Thickness Range: 10-400µm Adjustable,

10-50μm, 5μm increments

50-100µm, 10µm increments

100-400µm, 50µm increments Specimen Retraction: to 100µm Adjustable Horizontal Stroke: 1” Vertical Stroke: 1.75” Specimen Orientation: 8°C (X, Y, X Three Directions) Voltage: 110V±10%, 20AMP Frequency: 50Hz Power: 850VA Largest Start-Up Current: (5 sec) 45A Chamber Temperature: -10°C ~ -35°C Adjustable Freezing Shelf Temperature: -45°C Number of Freezing Stations: 18 Peltier Number: 2 Refrigerant: R404a, 300g±10g Compressor Oil: 0.6L EMKARATE RL-22S, ICI

Dimensions Width (w/o Hand Wheel): 25.5” Width (w/Hand Wheel): 30.5” Depth: 27.6” Height: 50” Weight: 390lbs

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4 | Installation Transportation/Unpacking • Unit must be shipped in the upright position. If the unit was shipped or stored lying down, it must stand upright for 36 hours before use so all lubricating oils enter the compressor. It should only be used after 4 hours of standing upright to ensure proper lubricant distribution.

• Inspect the package for damage. If damaged, do not open and immediately contact Tanner Scientific ® and the transportation carrier.

Site Requirements • Do not operate in rooms with or near flammable chemicals.

• Place instrument at least 10” from other instruments. Areas on both sides of the instrument should be clear to allow proper air flow to the cooling unit.

• Do not place in rooms above 75°F or in a humid environment directly in sunshine, OR NEAR AC VENTS OR CEILING FANS. The environment’s relative humidity should be no more than 80%. Failure to follow this procedure could create frost and icing in the chamber.

Placement • The cryostat is a movable floor model with 4 caster wheels and 2 stabilizing legs in the front that are adjustable to level the instrument.

Hand Wheel Assembly • Insert the pin of the hand wheel shaft into the hole of the hand wheel.

• Mount the spring washer on the screw (M10x15).

• Tighten the screw with a #6 Allen key.

Freezing Chamber Assembly • Place the waste tray in the freezing chamber.

• Install blade holder on the microtome base and lock.

• Place all tools needed for specimen preparation in the chamber.

• Close glass window.

• Connect power.

Check your power socket to ensure it is well grounded and that the power supply is stable, constant and accurate.

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5 | Operation

5.1

| Blade Holder

Lock Anti-Roll Plate Knobs

Clamping Lever For Locking

Clamping Lever Locks the Blade Holder (Moving Back and Forth)

Clamping Lever Locks the Blade Holder (Moving Left and Right)

Clamping Lever for Adjusting Angle Knob Adjusts Height of Anti-Roll Plate

5.1.1

Clamping Lever for Anti-Roll Plate

| Blade Holder Adjustment

Turn the blade holder lock handle clockwise to release it. Manually move back and forth and lock in the desired position. When the holder moves to the terminal point, the entire blade holder can be taken out and put back by reversing the procedure. Turn (counter clockwise) the blade holder lock handle. Manually move left and right and lock in the desired position.

Notice: Remember to lock the blade holder in the correct position after moving it forwards or backwards.

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5.1.2

| Changing the Blade

Release the clamping lever on the upper right to open the blade holder.

Insert blade from left to right and then lock it in place.

Note: Blade is extremely sharp. Use caution when handling or replacing blades.

5.1.3

| Adjusting the Angle of the Blade

Loosen the lowest adjustment handle on the right side of the blade holder.

Refer to the reference scale to turn the rotator in order to choose the best cutting angle of the blade. The angle should be no more than 10°. Retighten the adjustable handle.

Tissue

Anti-Roll Plate

Side Face of Blade

30

10° °

Adjustable Angle

Angle of Blade Edge

Tanner Scientific ® TN9000 • Semi-Automatic Cryostat 9

5.1.4

| Adjust Anti-Roll Plate Angle with Blade

1) Make the edge of the anti-roll plate parallel to the edge of the blade.

• Insert blade and lock the clamping lever.

• Lock two knobs.

• Use hexagonal wrench to make blade and anti-roll plate parallel.

• Fasten hexagonal screw.

• Turn knob to adjust height of the anti-roll plate.

2) Make the edge of anti-roll plate and the edge of the blade flush.

• Turn height turning knob to adjust height of the anti-roll plate.

• Adjust accordingly until you get quality sections.

Edge of Anti-Roll Plate and Blade Flush

Edge of Blade Edge of Anti-Roll Plate

Adjust with Hex Wrench.

Make the Edge of Anti-Roll Plate and Edge of Blade Flush.

1) The upper part of the anti-roll plate should not touch the specimen.

2) When the quality of the tissue section is lacking, check the edge of the blade for foreign matter.

3) Avoid touching the front of the plate.

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Anti-Roll Plate Height Turning Knob

5.2

| Specimen Disc Installation

• Adjust the specimen clamp accordingly.

• After the specimen has been frozen on the specimen disc, insert specimen disc shaft into the hole in the center of the clamp. Join the front surface of the clamp with the rear surface of the specimen disc. Rotate the screw to lock the front of the clamp.

• Loosen adjustable clamp screw, turn the lever to adjust the direction of the specimen disc surface and lock.

Specimen Disc Lock Screw

Adjust & Lock Clamp Screw

Clamp Adjust Lever

Specimen Disc

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5.3

| Freezing Specimen

The specimen freezing station is located at the left side of the chamber.

There are 18 holes to place the specimen disc, 2 of which are semiconductor refrigeration holes. The temperature of the freezing station is always colder than the freezing chamber’s temperature.

• At room temperature, cut the specimen to an appropriate size. Place on disc and fix with tissue freezing medium.

• Insert specimen disc with specimen into the freezing hole.

• Hold the freezing clamp on the specimen for about 30 seconds or until specimen is completely frozen.

• After specimen is frozen, insert specimen disc into the specimen clamp, lock, and begin sectioning process.

1) Movable Support 2) Heat Extractor 3) Specimen Hole 4) Specimen Disc

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5.4

| Defrost

In some situations, it makes sense to allow the machine to defrost and eliminate excess ice buildup. When the defrost cycle runs, ice will melt and drain into the waste container.

• The defrost cycle runs for almost 15 minutes.

• Upon completion of the defrost cycle, the machine will automatically return to its original cold state.

Automatic Defrost Timer Set the automatic defrosting time accordingly. When the chamber’s temperature reaches -5°C, defrost automatically terminates.

Instant Manual Defrost Set the manual defrost at any time. It can be turned off if needed.

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5.5

| Vertical View

Touch Screen Display

Touch Pad

Power Button

Lighting & UV Lamp

Hand Wheel

5.6

| Touch Screen Display (Work Area)

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5.6.1

| Temperature Setting

1) Actual Temperature (Reads the Chamber’s Current Temperature) 2) Set Temperature (Use +/- to Increase or Decrease Temperature) 3) Compressor “On” (Indicates if the Compressor is Running or Not)

Delay Startup Mode When the compressor’s downtime is less than three minutes, and is started again, the compressor will enter delay startup mode. The compressor will become fully operational after 3 minutes.

5.6.2

| Section & Trimming Section

1) Current Section Thickness (5µm) (Use +/- to Increase or Decrease) 2) Current Trimming Thickness (10µm) (Use +/- to Increase or Decrease) 3) Switch Key (Toggles Between Section and Trimming) 4) Thickness Setting Key (Adjusts Thickness) 5) Total Section Thickness (Calculates Total Amount of Section Cut)

Tanner Scientific ® TN9000 • Semi-Automatic Cryostat 15

5.6.3

| Start Freezing Stage

1) Temperature of Freezing Stage (Specimen Chuck Freezing Stage) 2) Freezing Timer (Reads How Much Time is Left in the Freezing Cycle) 3) Freezing Time (900 Seconds: 900S) Freezing will stop when countdown reaches 0.

Press Freezing Time button at any time to stop freezing.

Working Conditions 1) Freezing Stage Temperature < -10°C 2) Working Compressor On If these two conditions are not satisfied, electronic cooling automatically stops. Countdown will automatically restart when conditions are met.

5.6.4

| Lighting and UV Lamp LIGHT ON

UV ON

LIGHT OFF

UV OFF

Note: UV disinfection will only activate when the glass door is closed, which means the UV lamp can not work when the glass door is open. When the lamp is on, opening the door causes the light to automatically turn off. Close the glass door and press the UV button, the lamp will light up again.

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5.7

| Auxiliary Function Setting

Set the Time of Day the Cryostat Enters Defrost Mode and Sleep Mode.

5.7.1

| Defrost Setting

1) Manual Defrost 2) Automatic Defrost Settings from Sunday to Saturday.

Press square below each day to activate. Selected Unselected 3) Timer. Press the black square to display timer.

Choose hour and minutes.

Press to increase or decrease time.

Click SAVE or CANCEL to save or cancel the time setting.

Each defrost time takes 10 minutes.

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5.7.2

| Sleeping Mode Setting

1) Manual Sleeping Mode. Chamber should keep running at -5°C while the display light dims. To deactivate sleep mode, press the button at any time.

2) Automatic Sleeping Mode. Without any activity after two hours, the machine automatically enters sleep mode.

3) Automatic Sleeping Mode Start Time and End Time.

Note: After setting up the time, you need to press the SAVE button.

Otherwise, the setting will not enter sleep mode.

5.8

| Time Setting

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5.8.1

| System Time Setting

1) Select the box under the workday you want to set a time. refers to the corresponding workday you selected.

2) Select the black square to display time. Choose hour and minutes.

Select to increase or decrease time.

3: Press SAVE to save the setting. At the same time, the gray bar in the lower right corner of the screen will display your set time.

5.8.2

| Setting Retraction Value of Section

Select (+/-) to increase or decrease the retraction value. The range of retraction value is 0-100μm with 5μm increments. REMEMBER TO SAVE.

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5.9

| Course Feed Control Panel

TIP: Before adjusting any specimen, make sure the hand wheel is locked.

Located to the left of the main chamber, the course feed control panel allows you to advance or retract the specimen. Additionally, you can control the thickness by using the +/- buttons.

You can increase the speed at which the specimen advances or retracts by holding down the “F” button at the same time pressing the or buttons.

5.9.1

| Trimming

Trimming can be controlled on the display screen and the touch pad located to the left of the chamber. Use the +/- buttons to increase or decrease the trimming thickness with each turn of the hand wheel.

Trimming allows you to cut away unwanted portions of the specimen.

• Place the blade into the holder and lock. Adjust the anti-roll plate.

• Adjust the blade holder’s angle 1°~2°, or to the angle that works best.

• Adjust holder and sample, but keep enough space for depth of cutting.

• Open the anti-roll plate.

• Unlock the hand wheel.

• Move the sample close to blade. Turn the handwheel to section.

Thickness can be set to 20μm. Reduce gradually to the desired thickness.

• Close the anti-roll plate.

• Turn hand wheel to trim excess specimen away.

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5.9.2

| Sectioning Mode

• Press the “Switch” button to enter sectioning mode.

• Turn the hand wheel one rotation. The instrument will cut one piece of section at a time.

• It’s best to turn the hand wheel at uniform speed.

• Adjust speed until you receive quality sections.

5.9.3

| Sectioning Considerations

1) When using the cryostat, controlling the blade’s speed and correctly adjusting the anti-roll plate are important factors in cutting high quality sections. The proper speed of the blade can only be acquired by practice and experience, along with skillful control of the hand wheel. The proper adjustment of the anti-roll plate requires even more practice. Sometimes they interfere with each other and require careful adjustment.

2) When freezing tissue, the water within the tissue will condense into ice and the tissue will harden. The hardness changes with the temperature: the lower temperature, the harder the tissue becomes. Each tissue reacts differently under various temperatures. Achieving high quality sections can only be obtained through practice. Cutting temperatures of the specimen tissue without fat and formalin should be between -13°C ~ -25°C. 3) For high quality sectioning, pay attention to the following: • Proper working temperature in the chamber • Correct hand wheel speed • Fine adjustment of the anti-roll plate • Sharp blade edge and correct blade angle 4) Frozen sections should not be cut in wide sections. Cutting thinner sections, so that the line of contact is shorter, is ideal.

Tanner Scientific ® TN9000 • Semi-Automatic Cryostat 21

5.10

| Maintenance

5.10.1

| Daily Finishing Work

• Lock the hand wheel.

• Remove the blade from the holder accordingly.

• Remove waste from the chamber.

• Clean blade holder and brush holder.

Tip: General cleaning can be used with 95% ~ 98% alcohol.

• Close the glass window.

• Turn off the lights.

• Do not turn off the main switch. Do not stop the cooling system when the compressor is running.

5.10.2

| Power Up/Down

If you go several weeks or longer without using a machine, you can turn off the main power switch.

• Turn off the machine’s power switch when compressor is not running.

• Open the glass window, ventilate the freezing chamber and let dry.

• Remove all specimens from the freezing shelf.

• Lock the hand wheel.

• Remove the blade from the blade holder.

• Remove waste.

• Remove the dust pan and brush to clean.

Before restarting the machine, make sure all parts in the freezing chamber are thoroughly dry or icing could occur.

22 Tanner Scientific ® TN9000 • Semi-Automatic Cryostat

6 | Cleaning & Maintenance

Since these machines are precision instruments, it’s important to pay attention to cleanliness, maintenance and repair work. Do NOT use organic solvents or any aggressive substances to clean and sterilize the chamber! Only use the cleaning agents specified in this manual. If necessary, the external surface may be cleaned with light-duty commercial household cleaner.

Use a wet cloth and rub until dry. Do NOT apply xylene or glass cleaner.

6.1

| Daily Cleaning

• Dispose of any waste daily.

• Clean the waste pan.

• Clean the brush holder.

• Waste water treatment must comply with local environmental regulations.

• Observe the water level in the bucket and discard accordingly.

6.2

| Maintenance

• Clean the machine daily.

• Add droplets of low temperature lubricating oil on the plastic coupling interface every week.

• Lubricate the Specimen Feed Mechanism Weekly: Click the fast forward button to make the clamp move to the front position, drip a drop of the low temperature lubricating oil on the copper bushing, then click the fast rewind button to make the clamp move to the back position.

• Lubricate the Eyelet Cover As Needed: Turning the hand wheel, drip some drops of the low temperature lubricating oil on the decorative sheeting when the specimen clamp on the highest and lowest position, then turn the hand wheel again to make the oil distribute evenly.

• Clean the Condenser As Needed: Open the vent panel at right of the machine, clean the dust on the cooling fin of the condenser with brush, or jet it with high-pressure airflow.

Tanner Scientific ® TN9000 • Semi-Automatic Cryostat 23

6.4

| Replacing Fuses

• Turn off the machine and unplug the power cord.

• Replace the Fuse: There are 5 fuse boxes in the back of the instrument.

Open the cap and remove the broken fuse tube. Insert a new fuse into the lid. Close the lid on the fuse box and tighten.

Note: You can only use the same type of fuse!

1

2

3

1) Light 1.5A 2) Defrost 3A 3) Anti-Fog 3A 4) Compressor Overcurrent Protector The button bounces when there is too much electric current. Press button to restore.

5) UV Lamp 1.5A 6) Thermoelectric Cooler 5A

4

6.5

5

6

Note: The standard fuse is 6*30mm.

| Waste Bottle

The waste bottle is used to collect the liquid from defrosting tissue, washing fluid and waste.

Pour 200mL of 10% formalin solution or other disinfectant into the water bucket in order to prevent waste damage on personnel and environment.

Waste discharge should be in accordance with local regulations. Use correct methods to treat the waste water and waste.

24 Tanner Scientific ® TN9000 • Semi-Automatic Cryostat

7 | Warranty & Service Tanner Scientific ® warrantees this product for a period of one year from the date of purchase, provided that the customer complies with the rules of this user manual. Extended warranties are available. Contact Tanner Scientific® at 888.708.5233 for more information. Tanner Scientific ® is not responsible for any damage or other issues caused by abuse and misuse of the instrument.

Service Information

If you require any service or parts during the warranty period, please contact Tanner Scientific ® at 888.708.5233, or the sales agent who sold you the unit.

Prior to calling, please have the instrument model and serial number ready.

Amendment

Tanner Scientific ® reserves the right to change the technical parameters of any model for improving the function of the instruments.

Quality Guarantee • Tanner Scientific ® will ensure that every instrument sold has been strictly examined to ensure it meets the stringent quality and technical standards.

• Service terms are only provided for those who regularly use the instrument and operate the instrument according to its instructions.

Disposal of Unwanted Instruments

Discarding the instrument and its parts should be done according to current laws and regulations.

Tanner Scientific ® TN9000 • Semi-Automatic Cryostat 25

8 | Troubleshooting PR O B L E M S

C AU S E S

E L I M I N ATI O N

• Section melts

• Temperature of the specimen, blade, anti-roll plate or blade holder is not low enough

• Set lower temperature

• Section splinters

• Temperature of the specimen is not low enough

• Set higher temperature

• Temperature of the specimen is not low enough

• Set lower temperature

• Specimen is too big

• Trim the plane of the specimen and increase the thickness

• Relative position of the anti-roll plate’s edge and the edge of the blade is not correct

• Adjust the anti-roll plate

• Cutting angle is not correct

• Adjust the angle of the blade

• Blunt blade

• Use other part of the blade to cut or replace with new blade

• Although the temperature and the position of the anti-roll plate are correct, the section is uneven.

• Residue on the blade or the anti-roll plate

• Clean

• Section curls

• The clearance between the edge of the anti-roll plate and the cutting edge is too small

• Increase the clearance

• The clearance between the edge of the anti-roll plate and the cutting edge is too small or the edge of the anti-roll plate has touched the specimen

• Adjust the anti-roll plate

• Damaged blade

• Use other part of the blade to cut or replace with new blade

• The edge of the anti-roll plate has been damaged

• Use new anti-roll plate

• The temperature of the anti-roll plate is too high or its position is not correct

• Reduce the temperature and adjust the position of the anti-roll plate

• Anti-roll plate is electrostatic

• Remove electrostatic

• Residue on the anti-roll plate

• Clean

• Rusty blade

• Replace with new blade

• Section uneven

• Odd sounds when slicing

• Tissue section is sticking or broken on the anti-roll plate

26 Tanner Scientific ® TN9000 • Semi-Automatic Cryostat

PR O B L E M S

• Section elevates

• Tremor when slicing

• Section curls on the anti-roll plate

• Broken tissue section

C AU S E S

E L I M I N ATI O N

• The freezing temperature of the specimen is not adequate

• Continue to freeze

• Specimen disc is not fastening

• Review and lock in place

• The knuckle of the specimen clamp is not fastening

• Review and lock in place

• The blade is not fastening

• Review and lock in place

• The specimen is loose from the disc after slicing

• Retighten the specimen

• Section is uneven and too hard

• Increase the thickness and reduce the area

• Blunt blade

• Use other part of the blade to cut or replace with a new blade

• Wrong type of blade

• Use correct type of blade

• Wrong cutting angle

• Adjust the angle of cutting

• Wrong temperature

• Adjust the temperature

• Wrong type of blade

• Use correct type of blade

• The rotation speed of the hand wheel is wrong

• Adjust the speed and rotation

• Loose blade

• Review and lock in place

• The specimen is loose from the disc after slicing

• Retighten the specimen

• Blunt blade

• Use other part of the blade to cut or replace with a new blade

• Wrong cutting angle

• Adjust the angle of cutting

• The machine is not dry thoroughly before turning power on

• Make sure the machine is dry

• Specimen is too dry

• Use new specimen

• Influenced by the air or static

• Remove static

• Temperature of anti-roll plate is too high

• Reduce the temperature

• Temperature section is too low

• Increase the temperature

• Blunt blade

• Use other part of the blade to cut or replace with a new blade

• Residue on the blade or the anti-roll plate

• Clean

• The edge of the anti-roll plate has been damaged

• Use new anti-roll plate

Tanner Scientific ® TN9000 • Semi-Automatic Cryostat 27

Manufactured by Tanner Scientific 1107 Tallevast Rd Sarasota, Florida 34243 888.708.5233 Info@TannerScientific.com www.TannerScientific.com