Chapter 06

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C h a p t e r

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Whole Blood Collection and Component Processing Ram Kakaiya, MD, MBBS; Colleen A. Aronson, MT(ASCP)SBB; and Jana Julleis, MT(ASCP)SBB

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H E C O L L E C T I O N O F whole blood from the donor and subsequent processing of the donation into separate components are key links in the transfusion chain of events. Careful attention to proper technique is critical to ensure optimal care of both the donor and the recipient.

WH O L E B L O O D CO L L E C T IO N The phlebotomy staff involved in whole blood collection should be trained in proper collection technique to minimize contamination of the donated unit and to minimize phlebotomyrelated local complications such as hematoma or nerve injury. Phlebotomists and other (collection) staff perform those functions under the direction of the medical director through the use of approved standard operating procedures (SOPs). Phlebotomy should be performed only after the donor has been found

to be eligible for blood donation and the following items have been properly labeled using a unique unit identification (ID) number label: the blood donor record, the primary and satellite containers, and the sample tubes. A final check of appropriate labeling before phlebotomy ensures that the donor history data, laboratory data, and other manufacturing data are assigned to the right blood components. The final check can be documented by having the staff initial the donor record. Blood Containers Desirable Properties Blood containers used for whole blood collection must be approved by the Food and Drug Administration (FDA), must be pyrogen-free, and must be identified by a lot number. The desired properties of storage containers include the following: easily formed and processed, flexible, tough, kink-resistant, and scratch-

Ram Kakaiya, MD, MBBS, Medical Director, LifeSource Blood Services, Glenview, Illinois; Colleen A. Aronson, MT(ASCP)SBB, Director, Technical Operations, LifeSource Blood Services, Glenview, Illinois; and Jana Julleis, MT(ASCP)SBB, Director, Manufacturing Processes, Blood Systems, Inc, Scottsdale, Arizona The authors have disclosed no conflicts of interest.

189 Copyright Š 2008 by the AABB. All rights reserved.

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