Moticam Pro booklet

MOTICAMPRO P R O F E S S I O N A L

C C D

M I C R O S C O P Y

C A M E R A S

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MOTICAMPRO The Moticam PRO series contains 12 models with different SONY ICX sensor resolutions and technical characteristics, providing users with a wide variety of options to choose from.

It includes Colour CCD imaging sensors for conventional microscopy techniques, Monochrome CCD imaging sensors for low luminosity microscopy and Peltier cooled CCD Colour and Monochrome imaging sensors for fluorescence microscopy. The cameras are assembled and tested to the highest standards in the clean room at our factory.

All Moticam PRO cameras come with the universal c-mount thread and are connected to the PC via a USB 2.0 port. The Moticam PRO Series comes with the well known and user-friendly Motic Images Plus 2.0 analyzing software (multilanguage). Additionally with any Moticam PRO, the sophisticated Motic Images Advanced 3.2 is supplied free of charge. Furthermore the Motic TWAIN drivers, Direct Show drivers (via our website) and the SDK are included with the software package of the Moticam PRO.

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Peltier Cooling device When using a camera for a long time, the sensor gets warmer and warmer. When using long exposure times, it becomes more sensitive to noise and this can be seen in the captured image. With our Peltier mechanism, the sensor is cooled down up to 10°C below ambient temperature (ambient = the temperature inside the camera case). Peltier Cooled cameras are therefore recommended for fluorescence applications.

Live readout The cooled version of the Moticam PRO features a built-in sensor arrays that allows the user to see live updated information on the Sensor and Ambient Temperature, as well as the Relative Humidity inside the casing. Thus eliminating the possibility of condensation brought on by rapid cooling. Additionally the user can set a “Target Temperature�.

Colour and Monochrome Colour cameras use a RGB primary colour mosaic filter on the chip. The distribution is standard; 25% Red, 50% Green and 25% Blue. Most of the time when working in fluorescence only one colour is revealed; therefore not allowing all available pixels to receive information. Just imagine seeing a blue image through the eyepieces, this means that only 25% of the total pixels are receiving and distributing information.

The Monochrome cameras do not have any colour filter and therefore each pixel records the amount of light it sees. It does not transmit any information on the colour of the sample to the computer. For these reasons monochrome cameras can definitely be considered when working with fluorescence. You will have better chances of capturing low light samples when using the full capacity of the pixels in the chip. Of course colour is important in fluorescence, but this can be added at a later stage with software.

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MODEL

SENSOR SIZE

PIXEL SIZE

RESOLUTION

1/2”

4,65 x 4,65

1360 x 1024

ICX252AQ

1/1.8”

3,45 x 3,45

2080 x 1542

ICX282AQ

2/3”

3,40 x 3,40

2580 x 1944

2/3”

6,45 x 6,45

1360 x 1024

SONY SENSOR

A ICX205AK

MOTICAMPRO

205

B C ICX205AL

D

MOTICAMPRO

252

MOTICAMPRO

282

A B

A B

A ICX285AQ

MOTICAMPRO

285

B C ICX285AL

D

NOMENCLATURE

Monochrome

Colour

Peltier cooled

High speed

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Motic Images Plus 3.2 This software contains all functions of our well-known Motic Images Plus 2.0 software plus much more. Its main features are:

Motic Images Advanced 3.2 - Counting

Capture Auto Capture Video recording Measurements

Automated counting Creating reports Image comparison Amalgamation

What makes Motic Images Advanced 3.2 software a more sophisticated version are the following features: segmentation, multi-focus and assembly.

Segmentation

Motic Images Advanced 3.2 - Assembly

The superior counting module allows you to perform manual segmentations by using a histogram (colour), grey scale or by selecting the size. You can create in one sample different groups, which will be highlighted in different colours. After the segmentation has been performed, you will have information about the area in square Âľm, perimeter, width, height and much more details. The results of the segmentation can be exported in an excel file for further analysis.

Assembly This module can be used for creating a single overview image out of multiple separate images. The software recognizes any overlapping and corrects this automatically. This software is specially designed for users who wish to have an overview of the complete sample, while working at a higher magnification.

Motic Images Advanced 3.2 - Segmentation

Multi Focus The multi-focus module can be used to capture up to 100 images taken at different focusing levels. The software will automatically recognize the parts that are in focus in each image and assemble them into a new file. The result is a single image that is completely in focus. * Motic Images Advanced 3.2 is available in English language and is only compatible with PC.

Motic Images Advanced 3.2 - Selection

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Hardware • Sony ICX CCD imaging sensor • Motic control, processing & imaging boards • Built-in 4 frame buffer* • Built-in FPGA processor* • TTL trigger port* • Schott BG-40 filter • CS/C mount (c-ring provided) • USB2.0 data mini port (usb cable provided) • Universal power supply (for cooled cameras only) • Peltier cooling device (for cooled cameras only) • Cooling up to 10 degrees below ambient with temperature readout * Unlockable with SDK integration

Software • Motic Images Plus application software • Motic Images Advanced application software for Windows computer only* • Twain drivers • Motic SDK • Motic Direct Show drivers • Motic MI Devices live imaging module with live calibration grid, scale cross and scale bars * Available through free download

Minimum computer specifications • Intel 1GHZ • 1GB RAM • USB 2.0 • Windows XP (SP3) • Macintosh OSX

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Quantum Efficiency Diagrams Moticam Pro 205A

Moticam Pro 205C

& 205B G

0.8

R 0.8

B

0.6

Relative response

Relative response

0.6

0.4

0.2

0

400

500

600

0.4

0.2

0

700

400

Wave length

1.0

500

B

& 252B

B

900

1000

0.6

0.6

0.4

0.2

0 500

650

700

900

1000

& 282B

550

G R

0.8

Relative response

Relative response

R

600

650

0.4

0.2

0

700

400

Wave length

450

500

550

600

Wave length

Moticam Pro 285A

& 285B

Moticam Pro 285C

1.0

& 285D

1.0

0.9

R

G

0.9

0.8

0.8

0.7

0.7

B

0.6

Relative response

0.6

Relative response

800

1.0

G

450

700

Moticam Pro 282A

0.8

400

600

Wave length

Moticam Pro 252A

0.5 0.4 0.3 0.2 0.1 0 400

450

Wave length

8

&205D

1.0

1.0

500

550

600

650

700

0.5 0.4 0.3 0.2 0.1 0 400

500

Wave length

600

700

800

Unit Control Software (MI Device)

Available options Video Device

Connection of multiple devices at the same time (Selection of the active device)

ROI function

Region Object Interest (manual selection)

Exposure time

Manual / Automatic

Full screen

Automatic

White Balace

Set Method, color balance ajustment

Image adjustments

Gain / Offset / Enhance / Gamma

Storage Format

SFC / JPG / BMP / TIFF / DCM (Available on Motic Advanced 3.2)

Background Balance

Manual

Color correction

+/- 10 (RGB)

Histogram

Live window in real time

Filters Live Image

Invert / Gray / Emboss / Red / Green / Blue / Red reverse / Green reverse / Blue reverse

Edge detection

0 / 10

Sharpness

+/- 10

Remove Noise

4 Levels

Scale

Live Images, Horizontal and Vertical (user configurable)

Grid

User configurable

Scale Cross

X e Y distances (select position)

Record parameters

Single capture / Auto capture / Video Capture

Cooled Enable

Only avalaible with cooled cameras

Cooled Cameras

Internal Sensor with target window (Sensor temp / Ambient temp / Ambient humidity / Dow point)

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Moticam Pro

Moticam Pro

205A

205A 252A

282A 252A

282A 285A

285A 205B

252B 205B

CCD Sony ICX

CCD Sony ICX

1/2”

1/2” 1/1.8”

1/1.8” 2/3”

2/3” 2/3”

2/3” 1/2”

1/2” 1/1.8”

Features

Features

Resolution

Resolution

Total pixels

Total pixels 1392x1040

1.4 Mp

Number of active pixels Number of active 1360x1024 pixels Pixel Size (microns)

Pixel Size (microns) 4.65x4.65

Live display mode and capture size

Live display1360x1024 mode and capture size 680x512 320x256 Select Size (ROI)

Scan

Scan

Progressive

Maximun Frame Rate* Maximun Frame 10 frames/S Rate Buffer

Buffer

Integrated Filter

Integrated Filter

Bit Depth

Bit Depth

Shutter

Shutter

Lens mount

Lens mount

Exposure Time

Exposure Time

Data Transfer

Data Transfer

Power Supply

Power Supply

Peltier Device

Peltier Device

1.4Mp 3.2 Mp

3.25.0 MpMp

5.0Mp 1.4 Mp

1.41.4 MpMp

1.4Mp 3.2 Mp

1392x1040 2088x1550

2088x1550 2588x1960

2588X1960 1392x1040

1392x1040 1392x1040

1392x1040 2088x1550

2080x1542 1360x1024 (4:3) 2080x1542 2580x1944 (4:3)

1360x1024 2580x1944 (4:3) 1360x1024 1360x1024 (4:3)

2080x1542 1360x1024 (4:3) 2

4.65x4.65 3.45x3.45

3.45x3.45 3.40x3.40

3.40x3.40 6.45x6.45

6.45x6.45 4.65x4.65

4.65x4.65 3.45x3.45

1360x1024 2048x1536 680x512 1024x768 320x256 512x384 Select Select Size Size (ROI) (ROI)

2048x1536 2560x1920 1024x768 1280x960 512x384 640x480 Select Select Size Size (ROI) (ROI)

2560x1920 1360x1024 1280x960 680x512 640x480 340x256 Select Select Size Size (ROI) (ROI)

1360x1024 1360x1024 680x512 680x512 340x256 320x256 Select Select Size Size (ROI) (ROI)

1360x1024 2048x1536 680x512 1024x768 320x256 512x384 Select Select Size Size (ROI) (ROI)

Progressive Frame Readout 108.5 frames/S frames/S

Frame Readout

8.57frames/S frames/S

Progressive

Progressive

7 frames/S 15 frames/S 4 Frames

1510 frames/S frames/S

108.5 frames/S frames/S

4 Frames

8 bits / 12 Bits (Switchable 8 bits / 12 through Bits (Switchable MI Device, usable throughwith MI Device, application usable software with application that supports software 12 Bit) that supports 12 Continuous Variable-Speed Continuous Variable-Speed C-Mount

C-Mount

1/1000 to 6 sec

1/1000 to 6 sec

Mini USB2.0 connection mini USB2.0 connection 5V (USB)

Universal Power Supply Universal (5V) Power S

5V (USB)

Up to 10º Celsius below Up toambient 10º Celsius b 0 ~ 60º C

0 ~ 60º C

40% - 80% (35º) 40% - 80% (35º)

Operating Humidity

Camera Data Readout Camera Data Readout

Sensor Temperature,Sensor Ambient Temperature, Temperature, Ambient Relative Temperature, Humidity, Dew Relative PointHumidity, Dew Point 117mm (L) x 65mm117mm (W) x 62mm (L) x 65mm (H) (W) x 62mm (H)

Dimensions

Dimensions

Weight

Weight

Support Device

Support Device

Driver USB2 DeviceDriver / TWAIN USB2 / SDK Device / Direct / TWAIN Show/ Driver SDK / /Direct TriggerShow Port Driver (TTL) / Trigger Port (TTL)

OS Compatibility

OS Compatibility

Windows XP / Vista /Windows Seven and XP MAC / Vista OSX / Seven and MAC OSX

Software Suite

Software Suite

*under optimal illumination conditions

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Frame

Schott BG 40 Bandpass Schott BG 40 Bandpass

Operating Temperature Operating Temperature Operating Humidity

Colour

Colour

Colour

406 gr

406 gr

546 gr

546 g

Motic Images Plus 2.0 Motic (PCImages & MAC), Plus Motic 2.0 Images (PC & MAC), Advanced Motic3.2 Images (PC), Advanced TWAIN, SDK, 3.2 (PC), MoticTWAIN, Direct Show SDK,Drivers Motic Direct Show

)

282B 285B

285B 205C

2/3” 2/3”

2/3” 1/2”

285C 205D

285C 205C 1/2” 2/3”

2/3” 1/2” Monochrome

Monochrome

Peltier

285D 205D

285DPro Moticam

Moticam Pro

1/2” 2/3”

CCD2/3” Sony ICX

CCD Sony ICX

Peltier Monochrome Peltier MonochromeFeatures

5.0Mp 1.4 Mp

1.41.4Mp Mp

1.4Mp 1.4 Mp

1.41.4Mp Mp

1.4Mp 1.4 Mp

2588X1960 1392x1040

1392x1040 1392x1040

1392x1040 1392x1040

1392x1040 1392x1040

1392x1040 1392x1040

1360x1024 2580x1944 (4:3) 1360x1024 1360x1024 (4:3)

4.65x4.65 6.45x6.45

6.45x6.45 4.65x4.65

4.65x4.65 6.45x6.45

2560x1920 1360x1024 1280x960 680x512 640x480 340x256 Select Select Size Size (ROI) (ROI)

1360x1024 1360x1024 680x512 680x512 340x256 320x256 Select Select Size Size (ROI) (ROI)

1360x1024 1360x1024 680x512 680x512 320x256 340x256 Select Select Size Size (ROI) (ROI)

1360x1024 1360x1024 680x512 680x512 340x256 320x256 Select Select Size Size (ROI) (ROI)

1360x1024 1360x1024 680x512 680x512 320x256 340x256 Select Select Size Size (ROI) (ROI)

7 15 frames/S frames/S

1510 frames/S frames/S

Progressive

1015 frames/S frames/S 4 Frames

Resolution

1392x1040 Total pixels

Total pixels

Pixel 6.45x6.45 Size (microns)

6.45x6.45 4.65x4.65

Progressive

Resolution 1.4 Mp

1360x1024 1360x1024 (4:3) 1360x1024 Number of active (4:3) pixels Number of active pixels

1360x1024 1360x1024 (4:3) 1360x1024 1360x1024 (4:3)

3.40x3.40 6.45x6.45

Readout

1360x1024 Live display mode and 680x512 capture size 340x256 Select Size (ROI) Scan

1510 frames/S frames/S

1015 frames/S frames/S

4 Frames

Schott BG 40 Bandpass Schott BG 40 Bandpass

Continuous Variable-Speed Continuous Variable-Speed C-Mount

1/1000 to 6 sec

1/1000 to 6 sec

5V (USB)

546 gr

0 ~ 60º C

Buffer

Buffer

Integrated Filter

Integrated Filter

Lens mount

Exposure Time

Exposure Time

Data Transfer

Data Transfer

Power(5V) Supply Universal Power Supply Universal (5V) Power Supply

Power Supply

Peltier Device Up to 10º Celsius below Up toambient 10º Celsius below ambient

Peltier Device

0 ~ 60º C

Operating Temperature Operating Temperature Operating Humidity

Sensor Temperature,Sensor Ambient Temperature, Temperature, Ambient Relative Temperature, Humidity, Dew Relative PointHumidity, Dew Point 117mm (L) x 65mm117mm (W) x 62mm (L) x 65mm (H) (W) x 62mm (H) 406 gr

Bit Depth

Lens mount

40% - 80% (35º) 40% - 80% (35º)

546 gr

Scan

Shutter

5V (USB)

elsius below Up toambient 10º Celsius below ambient

Live display mode and capture size

Shutter

mini USB2.0 connection mini USB2.0 connection

Power Supply Universal (5V) Power Supply (5V)

Pixel Size (microns)

15 Maximun frames/S Frame Rate Maximun Frame Rate

bits / 12 Bits (Switchable 8 bits / 12 through Bits (Switchable MI Device, usable throughwith MI Device, application usable software with application that supports software 12 Bit) that supports 12 Bit)Bit Depth

C-Mount

Features

406 gr

Camera Data Readout Camera Data Readout Dimensions

546 gr

Operating Humidity

546 gr Weight

Dimensions Weight

Driver USB2 DeviceDriver / TWAIN USB2 / SDK Device / Direct / TWAIN Show/ Driver SDK / /Direct TriggerShow Port Driver (TTL) / Trigger Port (TTL)

Support Device

Support Device

Windows XP / Vista /Windows Seven and XP MAC / Vista OSX / Seven and MAC OSX

OS Compatibility

OS Compatibility

mages Plus 2.0 Motic (PCImages & MAC), Plus Motic 2.0 Images (PC & MAC), Advanced Motic3.2 Images (PC), Advanced TWAIN, SDK, 3.2 (PC), MoticTWAIN, Direct Show SDK,Drivers Motic Direct Show Drivers Software Suite

Software Suite

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