November 2013

Dear colleagues, our partners!

Asfendiyarov Kazakh National Medical University staff greatly appreciates You for the support in training of qualified specialists in medicine, public health and pharmacy as well as for collaboration in the field of higher education.

On December 2-4, 2013 KazNMU is traditionally holding “University Days” aimed to attract medical community of Kazakhstan to the world medical science achievements and practice, to expand joint scientific and educational activities with foreign partners, maintain close University collaboration with the representatives of professional associations, employers and directly with the alumni themselves.

In this regard I have the honor to invite You and Your colleagues to take part in the international scientific-practical conference “Contemporary trends in the world development of medical education and healthcare.

Possibilities for international cooperation“.

The world known scientists from Great Britain, USA and South Korea will take the floor on the first day of the conference. Two round tables with University partners and employers will be organized. The program on “Day of Faculties” includes more than 19 thematic conferences, 6 round tables, 3 master-classes, symposium in bioethics and the First Eurasian congress of pathophysiologists.

During the three-day-event various students’ arrangements, competitions, contests and exhibitions will be held in the university campus. The conference will bring together the world-renowned scientists from USA, Germany, Italy, Spain, Japan, South Korea, Vietnam, Turkey, Georgia, CIS countries.

We look forward to meeting You in our university and continuing productive cooperation to train qualified specialists and improve education quality in the Republic of Kazakhstan.

Aikan Akanov Rector of S.D. Asfendiyarov KazNMU Organizing committee Chairman

THE CONDITION OF PERIPHERAL BLOOD OF RATS, WHICH BADGERED BY HEAVY METALS

by Dolayev Zh., Nurmakhan E., Aryntai T…………………………………………..10

THE PREVALENCE OF SMOKING AMONG THE FIRST AND SECOND YEARS STUDENTS OF KAZNMU

by Azizhanov P…………………………………………………………………………………12

PHYTOCOMPOSITION

by Dauitova Zh…………………………………………………………………………………14

S.D. Asfendiyarov Kazakh National Medical University Brief history, main information and other facts about the leading medical university of Kazakhstan, Alma-Mater of plenty of famous Doctors.

By Victoria Samuilova, Print Editor of ISJM Page 4

Breaking News!

IMMUNOCORRECTION SYNTHETIC DRUG PILOT INFLAMMATION CAUSED BY THE BACKGROUND OF HEAVY METALS

International Conference dedicated to 35th Anniversary of WHO/UNICEF’s Alma-Ata Declaration on PHC (6-7 November 2013) Page 5

by Abydynova A……………………………………………………………………………….18

BIOSAFETY OF A PERSON IN ELECTROMAGNETIC ENVIRONMENT

by Nurdaulet Zholdasov, Islamzhon Karimov……………………………………..22

INFLUENCE OF ANTHROPOGENIC FACTORS ON HEALTH

by Ikramova S.E………………………………………………………………………………24 THE ANALYSIS OF CAUSES OF THE DIVERGENCE OF CLINICAL AND PATHOANATOMICAL DIAGNOSES BASED ON THE RESULTS OF OPERATIONAL AND SECTIONAL MATERIALS

by Abdimanap A., Momynov B………………………………………………………….27 ASSESSMENT OF ELECTROPHYSIOLOGICAL PROPERTIES OF THE MYOCARDIUM IN ALMATY METRO WORKERS

by Isamatov B., Lee E., Sultanova A., Kulmaganbetov M., Umithan B……32 CLINICAL AND LABORATORY FEATURES AND ANTIBACTERIAL THERAPY OF LISTERIOSIS IN CHILDREN AT THE CURRENT STAGE

by Dzharbolova A.N. Mazhibaeva A.K. Nurgayn N.N. Kenenbaeva L.A.

Mini‐course: HIV/AIDS of Dr. Shiu-Lok Hu (University of Washington, Seatle) Page 5

Global Doc Journalist of ISJM Shirin Ziro took an interview from Dr. Gregory Liftshesa, Professor of Tel Aviv Medical University (Israel). Professor is sharing his exciting experiences with the students, and you can find an article “... Each student has a personal dead!..” on the Special Guests Corner: “Global Doc” Page 8

Zhumabai R……………………………………………………………………………………35

INFLUENCE OF NORMOBARIC HYPOXIA ON METAL INDUCED DAMAGE OF BEHAVIORAL REACTIONS OF RATS

by Kulmaganbetov M.A…………………………………………………………………… 36

WE ARE ONLINE! Our website collects articles, breaking news and events. You can read our blogs, download past and current issues, and find way to contact us.

Follow us on Facebook and Twitter to be kept up to date with the latest scientific advances in KazNMU and beyond!

W.: http://www.isjm.kaznmu.kz/ FB: https://www.facebook.com/groups/1376985435853427/ Twitter: @ISJM_KazNMU

Announcements Invitation to the X International Multidisciplinary Congress "Neuroscience for Medicine and Psychology”.

(Sudak, Crimea, Ukraine, June 2-12, 2014) Page 40

“University Days-2013” Key Speakers:   

Professor Vikram Jha (the UK) Professor Joon-Shik Shin (South Korea) Professor Michael Z. Wincor (USA) Page 40

S.D. Asfendiyarov Kazakh National Medical University S.D. Asfendiyarov KazNMU is the first and one of the leading medical universities in Kazakhstan.

The University was founded in February 1931. In March of the same year there was the first intake of students and 2 months later it’s began students’ teaching.

In autumn 1931 there was produced a second intake of students. At that time operated only Medical Faculty and staff of the Institute in 1931 consisted of 5 professors, 4 associate professors, 13 assistants, and 2 teachers. The students studied at the time in 10 departments: Normal Anatomy, Histology, Hygiene, Chemistry, Physics, Biology, Dialectical Materialism, Political Economy, the Kazakh language, Physical Education.

There was the first issue of doctors in 1936. Later many of them headed the Department of Medicine.

In 1938 there was opened a Pediatric Department.

In 1951 there was opened the Pharmacy Department. Now they provide “Bachelor of Pharmacy” specialists with giving a qualification of “General practice Pharmaceutist”. The activity of a General practice Pharmaceutist includes organizational, marketing, technological and control and analytical functions. It is one of the leading Faculties in the field of Fundamental Researches in Pharmacology. Scientific researches on highly active “Kazkain” anesthetics were defended by a number of patentees and they were awarded the State Prize in the field of Science, Techniques and Education.

Sanjar Dzhafarovich Asfendiyarov (20 October 1889-25 February 1938 ) - S.D. Asfendiyarov is a social and statesman.

- Born in Tashkent, he is a son of a military translator . He studied in the Imperial Military Medical Academy n.a. St. Petersburg in 1912. Then he worked as a military doctor. He was a member of the First World War (08.1915-12.1915).

In 1959 there was opened Dentistry Department.

For the past half-century Department has trained 7,247 dentists. Now the Faculty is teaching more than 500 students and more than 100 medical interns.

During World War II Kazakh Medical University has been a great help. Already in the first days of the war to the front voluntarily left 84 teachers and 262 students.

For merits in the development of Public Health Medical Institute in April 1981 there was awarded the Order of the Red Banner of Labor.

In the 5th of July (2001) the University was attached the status of the Kazakh National Medical

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University by the Order of N.A. Nazarbayev.

Today in the University well-known scientists and teachers of Kazakhstan, Academician of the National Academy of Sciences of the Republic of Kazakhstan, the Russian Academy of Medical Sciences, Academy of Preventive Medicine of the Republic of Kazakhstan, the International Academy, honored workers of science and education, honored doctors and pharmacists work.

Sanjar Dzhafarovich Asfendiyarov

- The first rector and head of the history of the Kazakh State University named after Abai (1928-1931).

- Director of the Kazakh Medical Institute (1931-1933) - Head of History and Archaeological Commission of the Kazakh branch of the Academy of Sciences of the USSR (1933).

- Head of the Universities, science and libraries of Kazakhstan (1933-1934).

- Was arrested on 22 August 1937. Convicted February 25, 1938 by the decree of a Session of the Military Collegium of the Supreme Court in Almaty under Articles 58-1, 58-2, 58-8, 58-9, 58-11 of the Criminal Code of the RSFSR, was sentenced to capital punishment - death.

- Rehabilitated 27 May 1958 the Supreme Court of the USSR for lack of evidence.

There are functioning faculties in the university:  Faculty of General Medicine;  Medical Prevention Department;  Faculty of Dentistry;  Faculty of Pharmacy;  Faculty of Management;  Faculty of Postgraduate Education.

S.D. Asfendiyarov KazNMU have a cooperation with the Universities such as: Nagasaki University (Nagasaki, Japan), Asia Pacific University (Beppu, Japan ), Parkway College (Singapore), University of Perugia (Perugia, Italy) , University of Transdanubia (Krems , Austria ), Graduate School of Management (Barcelona, Spain), Kyrgyz State Medical Academy (Bishkek, Kyrgyzstan) , the National Medical University named after Bogomoltsev (Kiev, Ukraine), the National Pharmaceutical University (Kharkiv, Ukraine), Moscow Medical Academy n.a. Sechenov (Moscow, Russia), and other University staff are actively involved in doing research on international programs and grants.

Issue 03 / November 2013 | isjm.kaznmu.kz

Victoria Samuilova Print Editor of ISJM Student of the 4th year of General Medicine Faculty

Mini‐course: HIV/AIDS There was Mini‐course of Dr. Shiu-Lok Hu, Professor of Pharmaceutics and Adjunct Professor of Microbiology in S.D. Asfendiyarov KazNMU. Dr. Hu holds a B.A. degree with Great Distinction in Biochemistry from the University of California,

International Conference dedicated to 35th Anniversary of WHO/UNICEF’s Alma-Ata Declaration on PHC (6-7 November 2013)

Berkeley, and a Ph.D. in Molecular Biology from the University of Wisconsin. He completed his postdoctoral training in tumor virology in Dr. J.F. Sambrook's laboratory at Cold Spring Harbor Laboratory.

Conference is organized in close cooperation with World

The primary interests of his laboratory are HIV

Health Organization EURO and UNICEF.

pathogenesis and approaches for the prevention

Ever since the WHO/UNICEF Alma-Ata Declaration in

and treatment of AIDS. Currently, the staff

1978, strengthening the primary health care has

pursues three major research areas: design and

increasingly been considered to be of the greatest

evaluation of vaccines against HIV and related

importance

primate lentiviruses; structural, functional, and

for

improving

population

health

and

wellbeing, and building more equitable societies.

immunogenic studies of HIV-1 envelope proteins;

Primary Health Care is the first level of a health care

and

system whеrе people present their health problems and

He has developed a highly pathogenic isolate of

whеrе the majority of the population’s curative and

HIV-2, which upon inoculation in pig-tailed

preventive health needs are satisfied. Given its key

macaques induces rapid CD4 depletion and

characteristics, primary health care has never left the

AIDS-like diseases. They have used this model to

policy agenda. It is one of the major strategies to realise

show that post-exposure treatment with antiviral

the new European policy for health – Health 2020 – and

drugs, even of limited duration, is highly effective

to achieving the United Nation’s Millennium Development

in reducing viral load and maintaining normal

Goals (MDGs), such as reducing maternal and child

CD4 cell levels. They have also generated

mortality . Primary health care is also part of the universal

infectious and pathogenic molecular clones of

health coverage agenda of the World Health Organisation,

this virus, which will be used to define genetic

and the Secretary General’s Strategy Every Woman Every

determinants that contribute to the pathogenic

Child and the A Promise Renewed Initiative targets.

phenotype of this virus.

pathogenesis

of

isjm.kaznmu.kz | Issue 03 / November 2013

HIV-2.

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... Each student has a personal dead!..

I wonder how it’s like to study in the best medical universities in the World. I'm sure each of us at least once, but asked yourself this question. This week, our KazNMU provided us with a wonderful opportunity to attend the lectures of professor of anatomy with a worldwide reputation - Gregory Liftshesa. Professor Liftshes came from Tel Aviv Medical University (Israel). I was lucky enough to visit her three lectures, but after the first he held in English, I wanted to talk to him in person and learn more about how to learn the future doctors in Israel.

-Professor Liftshes, is it your first trip to Almaty? What are your impressions?

-No, I've been here before. This is my second visit. I note that I like it here very much. Large and beautiful city, is constantly evolving is not in place. A real metropolis!

-As for the students? Do students between Israel and Kazakhstan differences?

-Of course there is! Your students sit in on lectures robes, and our no. (Laughs) But seriously, all the same young shining faces, sparkling eyes, and passionate heart.

- We have a KazNMU credit system, which provides a fully independent student work. How this state of affairs at Tel Aviv University?

- We also practiced, but not to this extent.

There are independent works, but still most of the students spend with the teacher explains to them that the material in the form of lectures and laboratory exercises. We have a lot of attention paid to dissection. That is, the student must spend a lot of time studying the anatomy of a dead man.

This is one of the characteristics of the policy department. In addition, students learn to work with the obligatory medical equipment. It has a large hall, where there are various techniques such as X-rays, ultrasound and many more different. Future doctors learn about how to look different structures and organs in the images obtained with these devices. That being said "Living Anatomy". Here's how: students volunteers like we do, after all look like internal organs or the bloodstream. I note that the lesson is present experienced doctor who all shows.

Lecturer with the Department only helps him and explains to students the nature and outcome of the processes.

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Professor Dosayev T.M.: - It's just amazing. I was lucky enough to visit the Tel Aviv University and see it all with my own eyes. There is a separate, large room. For example, you have passed the upper limb. Then this activity is fully tested on ultrasound. Ultrasound besides image has cardiogram. This program allows you to distinguish venous blood vessel from color.

- Where did you get such a technique?

It's worth a lot of money. Does the state sponsors?

-We have a more complex system. Apart from the state, we are helping many large companies with a worldwide reputation. For example, “Phillips”. Because they see this as advertising his label. It is clear that more than one generation of doctors will be studying for data devices.

- I wonder how young people come to you in high school.

-We have three basic criteria for admission to the University. In this English - needless to say.

This is mandatory. The first criterion is your matriculation, i.e. it’s average. The second criteria is psychometry, it includes a plurality of components of general knowledge, such as mathematics, logic, English, biology. This exam lasts for five hours, during which the students get a lot of questions with multiple correct answers. A third test is relatively new. It is a very interesting test, a kind of performance. This is aptitude exam.

After all, you can know the subject well, can be an excellent student with a beautiful psycho test, but in my heart you cannot be a very good man, not inclined to help.

This exam lasts for several hours, a kind of interview. It goes in stages. In addition, there involved any unexpected situations with doctors, psychologists. For example, you go from office to office. There is elderly woman who snatched right before you. Question: What do you do with this?

Issue 03 / November 2013 | isjm.kaznmu.kz

- Stop and help, of course!

- Not all decide so. There are those who disdain it, and think only of their own affairs.

Each time there are new and unexpected situations. Professional actors make them, with whom our medical university has concluded a special agreement. All sessions are kept in the strictest confidence.

Such professional satisfaction is extremely important. Pay attention, because there are doctors with experience who know a lot but their humanity is poor. Even a term that originated “Treat not the person, and treat disease�. The idea is that a person should be treated exactly. After all, when it comes to a very sick man, he cannot very pretty, it can come from bad smells, it may be what that spot, because he is sick. And there it is important to behave as a doctor. He will approach him on his toes with a squeamish person. Or he will understand that the disease is one of the characteristics that normally prevents him and we must do everything possible to help the person.

- What methods are best for students to learn anatomy course?

- Very interesting question. It is not so easy to answer. Honestly, everyone cannot do all the same anatomy includes topographic science, i.e. where is that, what size, and how one relates to others. It is important that in addition to cramming, you turned and logic. We study the anatomy of the principle differs from yours. So I think you harder than our students in a certain respect. Each student on the first lesson in anatomy gets corpse, which is fixed in a certain way and is like a doll. It is not in formalin. Your bodies are in formalin baths, right? See, it's not a good fixing. Firstly a very heavy smell, hard work, attacks the eyes. Our focus is different. On solution which fixes the corpse, we have only a small addition of formalin, which prevents bacteria flourish. And it recorded with the help of special alcohols, so these bodies can lie directly on the table, not fretting eyes in special suits which are impregnated with this solution. Students have free access to his personal corpse 24 hours a day (!). They have a special pass, and they can to come and do it even day or night. Before exams students live there. There's also a museum. But it is small. Your osteological museum makes me

envy (smiles). Our museum has a lot of different drugs. As you can see this greatly facilitates student preparation, i.e. it is easier to imagine the whole picture. Do not just memorize, namely to see. You should operate several types of memory. I would advise you another method involving computer programs. For example, anatomy TV. At the lesson, students under the supervision of the teacher carried out an imaginary dissection on the corpse. It is very convenient and allows practice. That is what I hope will facilitate your preparation. And most importantly you will understand perfectly.

- Your wishes to our University.

- Firstly, I want to say that you are very nice people, wonderful teachers! I wish to further improvements in teaching and the development of science. I see that people have interest and motivation. Thanks for the invitation; I was pleased to once again visit your University. Sure, our universities have much to learn from each other and cooperate.

- Thank you for the interview and for useful advice, surely they will help many of our students!

As it flew incredibly fast thirty minutes of our conversation with Professor Liftshesom, and it is time to read the next lecture. It is a wonderful experience, wonderful example of international cooperation. It would be wonderful, if we could learn from Israel, the various positive aspects, I think you've seen it for yourself. By the way and our KazNMU not standing still and is constantly moving forward leaving on an international level. I want to express my gratitude to our rector Akanovu Aykanu Akanovich, who gives us a wonderful opportunity to attend the lectures of professors with a Worldwide reputation.

Shirin Ziro Journalist of ISJM Student of the 2nd year of General Medicine Faculty

isjm.kaznmu.kz | Issue 03 / November 2013

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ISJM THE CONDITION OF PERIPHERAL BLOOD OF RATS, WHICH BADGERED

BY HEAVY METALS Dolayev Zh., Nurmakhan E., Aryntai T. E-mail: zharas92@mail.ru Supervisors: Professor A.N. Nurmukhambetov, Associate Professor M.K. Balabekova, Department of Pathological Physiology Kazakh National Medical University named after S.D. Asfendiyarov

Purpose of study Under the conditions of anthropogenic pollution of the environment a person is exposed to combined effects of metals. Due to the rapid development of road transport, oil-producing and chrome industry, high prevalence and prioritize of environmental pollution takes lead, chromium and vanadium. The toxic effects of many xenobiotics decrease the number of red and white blood cells. The condition of the hematopoietic system of the body can be considered as markers of adverse environmental impact. In connection with the above, the purpose of this research was to study the combined effects of chromium and vanadium to the blood system of adult rats.

Materials and methods The experiments were performed on 20 white male rats weighing 180-220 g, which were kept under the standard conditions of vivarium on a normal diet. Two series of experiments were performed: the 1st control animals, and the 2nd - experimental animals, which received potassium dichromate (BC) and ammonium vanadate (VA) orally for two weeks, using a metal probe at a dose of 5 mg / kg. Control animals received an equal volume of saline.

Experiments on animals were performed with compliance of bioethical rules.

Determination of indicators of rats’ peripheral blood was performed at the medical center "Immunodiagnostic".

Determined: the total number of

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leukocytes, the absolute and relative number of lymphocytes, neutrophils, erythrocytes, hemoglobin, color index (by conventional methods). Staining the smears of bone marrow by RomanowskyGiemsa was performed in the laboratory of pathomorphology in Science Center of Surgery named after A.N. Syzganov. And also was consulted in the Scientific Center of Pediatrics and Pediatric Surgery MoH. Procedures for statistical analysis were performed using the statistical packages as SAS 9.2, STATISTICA 10 and SPSS-20. The critical level of statistical significance in testing the null hypothesis was taken to be equal to 0.05 or 0.1. In case of exceeding the achieved level of significance, the null hypothesis was accepted.

Descriptive statistics are presented in the text as M Âą s, where M - mean, and s standard error of the mean. The significance of the results of research were taken in cases of p <0.05.

Results and Discussion The values of variables in the control and experimental groups were compared to reject the null and to accept the alternative hypothesis. This values were selected from general set of variables, using criterion of Wilcoxon, Kruskal-Wallis, Van der Waerden.

The normality of data distribution was taken if value of p higher than 5%.

As a result of two-weeks badgering with heavy metals compounds in dose of 5 mg/kg, the total number of leukocytes in

Issue 03 / November 2013 | isjm.kaznmu.kz

Issue 03 / November 2013 peripheral blood of animals was reduced on 40.2%, mainly due to the absolute and relative number of lymphocytes. So, the average value (s) of the relative amount of lymphocytes of badgered rats was 60.5 (7.6) that is less for 22.6% (p = 0.0001) from control level. The absolute number of lymphocytes reduced for 55,3% from control level. Increasing of relative and absolute amount of monocytes in blood of experiment rats was statistically significant (Ń&#x20AC;=0,0006).

The average content of erythrocytes and hemoglobin (6.1 (0.2) and 12.4 (1.1)) of badgered rats compared with those of control animals (at a significance level of p = 0.0001) was reduced by 24 7% and 12.0% respectively. The coefficient of variation (CV) of erythrocytes equal to 15.2% of the control animals was more variable than in the experimental animals.

While hemoglobin variability (CV = 8,8%) was 3.7 times higher than the control (CV = 2,4%).

Differences of color indicators for control and experience were not statistically significant.

The cytological research of the femur puncture showed that hematopoietic organs of control rats contained round nuclear cells, neutrophil and eosinophil myelocytes, polynuclear neutrophils and eosinophils, normoblasts and giant cells.

The main mass of cells in the bone marrow consisted of round nuclear cells and polynuclears. Mononuclear cells were quite varied, their small nuclei were located centrally or eccentrically, in some cases, completely fills the cell.

Neutrophil granulation was not very rich, but eosinophilic - well expressed.

Basophils had a round or lobate core.

Giant cells with a homogeneous plasma

and numerous central lying small nuclei were seen rarely. Normoblasts had typical nucleus and protoplasm. Nucleus of eosinophils and neutrophils are formed on the type of ring.

Cytological research of the bone marrow of experimental rats revealed heamotoxical effect of vanadium and chromium. So, the punctate of bone marrow was meager, represented by singe granulocytic cells without nuclear. Megakaryocytes was not revealed.

The combined using of salts of heavy metals led to hypoplasia of bone marrow due to reduction of immature and mature granulocytes, lymphocytes and megakaryocytes.

Conclusion Thus, it can be concluded that the effects of heavy metals in the animals was accompanied by development in peripheral blood lymphopenia with leukopenia, anemia, hypoplasia of bone marrow due to reduction of mature and immature granulocytes, lymphocytes and megakaryocytes.

Literature 1.

Trachtenberg I.M, Tychinin V.A, Talakin Y.N . The problem

of exogenous toxic effects of low intensity / / 2 Journal of Medical Sciences. 1991. - â&#x201E;&#x2013; 2. - P.5-12.

2.

E.L Levitsky, Gubskii Y.I, Marchenko A.N Primak R.G, A.G

goryushko . Correction of defeats of the nuclear genome with antioxidants in toxic liver damage / / Modern. Toxicology. -1998.

2-N. -p. 16-17.

3.

Eropkin M. Smirnova, T.D, E.M Yeropkina. Metabolic

activation with minimal toxic doses of xenobiotics as an acute pattern of acute cytotoxic Answer of human fibroblasts in culture / / Toxicol.vestnik . -1999.vesttnik -N 1. -p 16-21 4.

Galiyev S.A, Gallo R.S .Effect of exhaust gases on the

adaptive reactions of sensitized body. Materials of the International Conference "Tatishchev read: actual problems of science and practice of Togliatti, May 17-20, 2006 / / Togliatti. 2006 - P.89-91

isjm.kaznmu.kz | Issue 03 / November 2013

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ISJM THE PREVALENCE OF SMOKING AMONG THE FIRST AND SECOND YEARS STUDENTS OF KAZNMU Azizhanov P. E-mail: tkim595@gmail.com Supervisors: Associate Professor Zhuiko N.V., Ryspekova N.N., Kim T.A., Department of Pathological Physiology Kazakh National Medical University named after S.D. Asfendiyarov

In the modern world, tobacco is one of the most important causes of morbidity, disability and premature death, which defines the problem of tobacco as a global [1]. If the trend of increase in smoking prevalence does not decline, according to WHO in 2030 every year the number of deaths will be 8.3 million people by this reason, which represents 10% of all deaths in the World. Every year from diseases related to smoking, more than 25 000 Kazakhs are dying.

According to the WHO, over the next fifteen years, Kazakhstan will lose two million smokers. According to statics, more than 27 per cents of Kazakhs smoke which is 4.2 million people. In Almaty around 350 000 people smoke [2].

Medical studies have shown a clear link to smoking with diseases such as chronic bronchitis, lung cancer and emphysema, coronary heart disease, myocardial infarction, stroke, occlusive disease, etc.

[3].

In connection with such disappointing statistics the Kazakh President, Nursultan Nazarbayev, signed the laws such as "On prevention and curbing smoking" (2002), "On the prohibition of smoking in public places" (2009). In 2010, a state program of health "Salamatty Kazakhstan" for 2011-2015 was adopted. In addition, in 2006 Kazakhstan ratified the WHO Framework Convention on Tobacco Control (FCTC) [4,5,6,7].

According to these laws, and given the fact that the medical officer is a key figure in the formation of a healthy way of life, in KazNMU n.a. S.D. Asfendiyarov the internal rules prohibiting smoking in rooms and in the territory of KazNMU were adopted. For the systematic (more than three times) smoking on the premises of the University a student is a subject to expulsion.

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The purposes of research: To study the prevalence of smoking among KAZNMU’s students The objectives of research:  To analyze the data of the prevalence of smoking among younger students of KazNMU from faculties such as preventive health affairs, pharmacy, dentistry.  To promote the formation of an active professional attitude to smoking among medical students of the University. 

The method of research A survey specially designed questionnaire containing 17 questions. For all questions there were from 2 to 5 choices. A total number of questionnaires were 298 students from 1-3 courses, which were 117 students from preventive health, 121 from the Faculty of Pharmacy and 60 from Faculty of Dentistry.

During the survey it was found that all the students who participated in the survey were well aware of the negative effects of smoking on human health. The most part of the students of the Faculty of Dentistry agree that smoking has a negative impact on the processes of memory, attention weakens, impairs performance. However, the highest percentage of smokers at the time of the survey was at the faculty of Dentistry. The low percent of smokers was among the students of pharmaceutical and medicalprophylactic faculty. Moreover, according to the survey, the students of these faculties had previously smoked but do not currently suffer from this addiction anymore.

According to the survey the main motivation to smoke was often a stress reliever, sedative effect and the existence of "a smoking environment."

Issue 03 / November 2013 | isjm.kaznmu.kz

Issue 03 / November 2013 Results

Literature:

The analysis of the prevalence of smoking among students of the 1st, 2nd, 3rd year courses of medical-prophylactic faculty revealed that most of the smoking took place among the 3rd year students.

Findings:  The first year students of KazNMU have a high level of awareness about the negative effects of smoking  The highest percentage of smoking students according to the survey is among the Faculty of Dentistry  According to the survey the number of smokers among the 3rd year students of the Faculty of "Medical and prophylactic cases" is higher compared to the first and second years students  The majority of the students support the slogan "Smoking and the doctor are incompatible" and want to quit smoking soon

1. B. Zhusupov, G. Alimbekova Smoking as a social problem in Kazakhstan. From the digest Lifestyle and health of newly independent states (otv.red. H.Haerpfer, D.Rotman, S.Tumanov – Mn. ..: 2003 – 308 p.) P.187-194.

2. M. Just-Sarobe. Smoking and the skin // Actas DermoSifiliograficas (English Edition). – 2008. – Vol.99, Issue 3. – P. 173184 3. B. Saltman and J. Figueras Health system reforms in Europe.

Analysis of modern strategies: Trans. from English. – M.: GEOTAR MEDICINE, 2000 4. Codex of the Republic of Kazakhstan about people's health and the health care system (with the amendments as of 19.01.2011) http://www.zakon.kz/zdorov_kodex.html 5. The Law of the Republic of Kazakhstan "On prevention and curbing smoking" of 10 July 2002 N 340, Bulletin of the Parliament of the Republic of Kazakhstan, 2002, N 16, p. 149. Additions made to the the Law of the Republic of Kazakhstan "On the prohibition of smoking in public places» http://www.zakon.kz 6. State Program for the Development of Health of the Republic of Kazakhstan "Salamatty Kazakhstan" on 2011 - 2015 years, http://www.mz.gov.kz

isjm.kaznmu.kz | Issue 03 / November 2013

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ISJM PHYTOCOMPOSITION Dauitova Zh. E-mail: zhadyra.dauitova@gmail.com Supervisor: Associate Professor Balabekova M.K., Department of Pathological Physiology Kazakh National Medical University named after S.D. Asfendiyarov

Actuality

Materials and methods

One of the most popular theory of aging is free-radical theory (1,2,3,4,5,6 ].

Mitochondrial active forms of oxygen (AFC) are an important factor in determining aging speed (7.8 ). This concept states that the intensity of oxidative stress increases with age as a result of an imbalance of pro-and antioxidative systems [3] and with age, the degree of oxidative damage of DNA macromolecules, proteins, lipids, carbohydrates increases and antioxidative action activity reduces. The theory explains not only mechanisms of aging, but also the emergence of many age-related diseases, such as cardiovascular diseases, diabetes, atherosclerosis, neurodegenerative disease, tumors, immunosuppression, etc. [3, 5, 6, 8, 9]. However, the literature on the anti-aging effect of natural antioxidant is controversial [3, 5, 10, 11, 12]. Currently, natural and synthetic antioxidants not found a wide application in the clinic [13].

In the meantime, use herbal medicines in anti-aging are still not sufficiently learnt (15). Therefore, we have attempted to investigate the impact on the processes of aging experimental rats, phytocomposition drug developed by us, representing the oil balm from medicinal herbs (pumpkin, germs of wheat, nettle, sea buckthorn, palm in a certain proportion).

Experiments conducted at 40 white rats, as contained in the standard terms and conditions in vivarium and on a normal diet.

Held 4 series experiment: 1-series - mature bulls and rams rats 10 to 12 months, weight 200-240g, exposed immobilization stress; 2 series - old rats older than 22 months of age, weight 340-370 g; 3 series - the old rats as , the same age, exposed immobilization stress, 4 series - old rats that have been exposed immobilization stress, and treated with phytocompisition. (Stress in rats simulated intense transactions using premises animals in close plastic camera exposure control 30 min. In a period of one week. After the immobilizied stress the animals of all series experiment studied behavioral reactions in the tests "open fields", Morris water maze, CRAA (conditioned reflex of active avoidance) and PA (passive avoidance). After a week had been repeated studies behavioral reactions.

The test "open field" define the measurement of behavior, caused by placing animal in unfamiliar open space, flight from which it is impossible. For the water labyrinth used swimming pool filled with water, without soy milk, one end of which was placed a safe platform. Rat allowed to fly in the opposite end of the pool and noted the time of searching the platform. Then animals trained conditional reactions active and passive human joy (CRAA and PA) and within a week was tested behavioral reactions and securing memory about Hubbard (PES) (8). When using the CRAA animals in both first and second experienced sessions produce on 50 combined conditional won't go, counting the number of avoidance response and rid. When checking the RA maximum time observation was 300 sec.

The statistical analysis was performed using

Purpose of study In connection with the above, the purpose of the present study was an examination of the effects of the phytocomposition to behavioral reactions in animals different age groups in conditions of exposure immobilization stress.

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Issue 03 / November 2013 the application package SPSS-16. The value of the statistical significance of taken in cases p< 0.05.

The critical importance of statistical significance of the test zero hypothesis was taken equal to 0.05, or 0.1. In the case of exceeding the achieved level of statistical criterion of that value, zero hypothesis has been taken into account.

Discussion of the results Under the influence of phytocomposition in the first pilot session old stressed rats committed in 2 times more moving on an external ascii files field, than young stressed specimen (tab.1). If the time of grooming in an older stressed rats to 1.5 times longer than the time of grooming, perpetrated by young stressed specimens, the under the influence phytocomposition this act behavioral reactions returned to the original level of young.

At the time, as behavior of stressed young and old rats act as “ a-pillar to the rear sweep” and “ duration stands without support” were absent, in rats treated with the phytocomposition these acts were at the level intact old rats.

In the second test session old rats , treated with phytocomposition the numbers og crossing outer squares increased by 28.6 % and 200% (mu=9, interquartile magnitude 5 to 20), than untreated stressed old (mu=7, interquartile magnitude 5 to 9) and young animals (mu=3, interquartile magnitude from 2 to 8). However, if the time of grooming the remaining animals in the second test session had tended to decline, the rats, treated with phytocompoition, it remained at the same level.

Thus, phytocomposition has provided a more expressed by corrective influence in the first term study in relation to the vertical thruster activity and emotional component behavioral reactions stressed old rats, whereas through a week of worrying about only horizontal movement.

The results of a study on hole exploratory behaviour have shown that, under the influence of phytocomposition animals during a PA avoid bright light in the 22 second cycles (interquartile magnitude from 6.5 up to 72) Research , whereas the old intact rats at 30.1 second cycles (interquartile magnitude from 19.5 up to 49.2 ), that it was the 36.8 per cent more (table 2).

The CRAA showed that the old rats, treated with phytocomposition, did not commit a single correct response avoidance. However, during the test the number of correct response choice had approached values intact old animals, whereas in relation to young and old stressed animals exceed the 58.2 per cent and 85.4 per cent ( table 3).

As it shown in the table 4, In the first test session in the water maze in an older stressed rats under the influence phytocomposition significantly improved the time finding a safe platform as compared to all other animal groups. For example, the search for the safe platform for they have decreased in relation to the young intact animals to 129.7 per cent, to stressed young rats - at 102.4 per cent, to old intact - at 264.9 per cent, and the stressed old rats - at 37.8per cent.

In the second test session reduction in search time, treated animals in relation to intact young and old animals and to stressed young rats was more pronounced on the 77.8, 124.2 per cent and 27.4 per cent, respectively, that had been pointed out to the significant impact corrective phytocomposition spatial memory in animals.

In this way, under the influence of phytocomposition in the old intact rats exasperated over species was less pronounced, and complemented by a horizontal and vertical spectrum of research activities. In the old stressed rats treated with phytocomposition, horizontal movement activity was in 2 times higher than in young stressed rats. If the behavioral reactions of stresed young and old rats is absent such acts as vertical research activity, in the rats treated with phytocomposition these acts were at the level of intact old animals. The intervention was more corrigent action in rats, which have been subjected emotional stress, in the first term.

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ISJM Phytocomposition, without affecting the learning processes and memory in passive avoidance reactions, significantly accelerated learning processes once again acquired skill in active avoidance reactions. The search for a safe platform in the water maze in the first pilot session was significantly shorter than in all groups by comparison. Thus, under the influence of phytocomposition the orientation in space of external benchmarks significantly improves.

However, this effect can be the consequence of emotional stress.

References 1.

Harman D.The biologic clock: the mitochondria. J. AmGeriatrSoc. (1972), 20:145–7.

2. Jan Gruber, Sebastian Schaffer, Barry Halliwell, The mitochondrial free radical theory of ageing – Wheredowestand & Frontiersin Bioscience, (2008), May 1, 13, 6554-79.

3. Sastre J., Pallardo F.V., Garciadela Asuncion J. еt al.

Mitocondria, oxidative stress and aging. Free Rad Res. (2000), 32:189–98.

4. Beckman K.B., Ames B.N. The free radical theory of aging matures. Physiol Res. (1998),78:547–81.

5. Shringarpure R., Davies K.J. Protein turnover the proteasome in aging and disease. Free Radic Biol Med. (2002), 32:1084–9.

Table 1. - The main statistical indicators behavioral reactions of young and old rats in the test "open field", subjected to immobilized stress and treatment with phytocomposition.

No.

1 2 3 4

The behavioral act

Number of outer square Number of racks on the rear.sweeps Dur.stands without support The time of grooming

Young + stress Mu(25 % -75 %)

Old intact Mu(25 % -75 %)

The first experienced session 5 (2-22) 13 (10-24V DC )

Old + stress Mu(25 % -75 %)

Old + Stress + PhC Mu(25 % -75 %)

15(9-27) ***

10(5-15) *****

0 (0-3)

1 (0-4)

0 (0-7)

1 (0-2)

0 (0-6)

2 (0-7)

0 (0-20)

2 (0-3)

18 (7-21)

35 (29-45)

27 (18-55)

19 (0-55)

The second experienced session Nuber of outer 3 (2-8) 12 (5-36) 7 (5-9) 9 (5-20) square 2 Number of racks on 0 (0-1) 1 (0-2) 0 (0-6) 0 (0-1) the rear.sweeps 3 Dur.stands without 0 (0-3) 2 (0-4) 0 (Offsets 0-14) 0 (0-2) support 4 The time of 12 (2-17) 28 (22-36) 4 (2-14) 20 (0-28 0-28 0grooming 28) Note: * - statistically significant in relation to the formulation, *** -young stressed to the old stressed, **** -old stressed to intact old, ***** -PhC to the old stressed 1

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Issue 03 / November 2013 Table 2. - Statistical indicators PA in young and old rats, subjected to immobilization stress and treated with phytocomposition.

No. 1 2 3 4

Series Young intact Young stressed Old intact Old stressed

Training Mu(25 % -75 %) 6 (4-10) 10 (4-11) 30.5 (19.5 -49,2) 9 (6 -17,7)

Check Mu(25 % -75 %) 300 (300-300) 300 (23.9 -300) 300 (264.5 -300) 300 (300-300)

Old stress + PhC 22 (6.5 -72) 300 (300-300) Note: * - statistically significant to the young, ** - to the young stressed, *** - The old ones

Wilkоxоn test (Level quantity.)

0.012 * Z =-2,527 0.002 * Z =-3,066 0.002 * Z =-2,521 0.002 * Z =-3,063 0.041 *** Z=-2,041 0.002 * Z =-2,521

Table 3. - Statistical indicators of CRAA (conditioned reflex of active avoidance) in young and old rats, subjected to immobilization stress and treated with phytocomposition.

No.

Series

Training Check Wilkоxоn test Mu(25 % -75 %) Mu(25 % -75 %) (Level quantity.)

1 Young stressed 0.12 (0) 6,9(0,25-17,8) * * 2 Old intact 7.4 (4.7 -9-8) 17.8 (12.2 -20,8) * ( ** ) 3 Old stressed 5.8 (0-8) 2.4 (0 and 4.75. *** 4 Old stress + PhC 0 (0) 16.5 (12.5 -22,7) * ( **** ) Note: * - statistically significant in the elaboration, ** -to the young stressed, * ** - to old intact, **** - the old stressed

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ISJM IMMUNOCORRECTION SYNTHETIC DRUG PILOT INFLAMMATION CAUSED

BY THE BACKGROUND OF HEAVY METALS Abydynova A. E-mail: aygerim.askarovna@mail.ru Supervisors: Professor A.N. Nurmukhambetov, Associate Professor M.K. Balabekova, Department of Pathological Physiology Kazakh National Medical University named after S.D. Asfendiyarov

Actuality It is known that the course and outcome of many pathological processes depends on the condition of non-specific resistance of the organism]. The main mechanism of protection of the human infections is inflammation.

Logic inflammation is evident and eliminating the damaging factor and restoration of damaged tissues. In ideal conditions, conjugation of these resistance mechanisms allows the body to successfully fight the infection and leads to a rapid recovery. Unfortunately, in reality diseases often observed depression of one or more immune defense mechanisms that lead to chronic processes, and in severe cases, the death of the organism. Most often as a result of anthropogenic activity disrupted the function of the immune system, leading to a decrease in the effectiveness of antimicrobial protection .

In this regard , it was of interest to study the efficiency of the drug IHF -2 in experimental inflammation in the background metallindutsirovannoy depression of immunological defense. In JSC "Institute of Chemical Sciences n.a.

A.B. Bekturova " synthesized 1 - (2ethoxyethyl) - 4 - ( dimethoxyphosphoryl ) - 4 - hydroxypiperidine , which has an immunomodulatory effect and used under laboratory code IHF 2.

Materials and methods The experiments were performed on 120 white male rats weighing 180-220 g , kept under standard vivarium conditions on a normal diet. Conducted a series of experiments 4 : Episode 1 - control animals , Episode 2 - intact animals with inflammation , and 3 series - animals

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with inflammation against the toxicity of ammonium vanadate ( VA) and potassium dichromate ( BA) (BA + BC + turpentine ) 4 series - animals treated with the IHF -2 against intoxication by metal salts ( VA + BC + + turpentine IHF -2). Metal salts of intoxication caused by the introduction of BA and BC in a dose of 5 mg / kg bw oral gavage for two weeks. After the two-week seed BA and BC animals aseptic inflammation caused by subcutaneous administration of 0.3 ml in mineral oil of turpentine in the interscapular region, then treatment with the IHF -2 at 50 mg / kg by dissolving in physiological saline and administered subcutaneously in 0.5 ml of a week. The animals were taken from the experiment by decapitation after administration ether anesthesia at 1, 7 , 14, 30 from the beginning day of the experiment correction IHF 2 .

The medical center " IVD " was determined phagocytic activity ( FG ) and NBT - test ( nitroblue tetrazolium reduction test ) , the test PPN (direct damage of neutrophils ) in a manner Fradkin (1985 ) [5,6 ] .

Procedures for statistical analysis were performed using the statistical package SAS 9.2, STATISTICA 10 and SPSS- 20. The critical level of statistical significance in testing the null hypothesis was assumed to be 0.05 or 0.1 . In case of exceeding the level achieved statistical significance criterion of this value , accept the null hypothesis .

Checking the normality of the distribution of quantitative traits in selected comparison groups was performed using the Kolmogorov-Smirnov Shapiro- Wilk test , Cramer- von Mises and Anderson-Darling .

For comparison, the central group settings used parametric and non-parametric methods : analysis of variance , including with the Kruskal â&#x20AC;&#x201C; Wallis test and the

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Issue 03 / November 2013 Wilcoxon rank- marks , the median criterion and the criterion of Van der Waerden . For all quantitative traits in the two groups was evaluated arithmetic and RMS ( standard ) errors mean and coefficient of variation. Descriptive statistics are presented in the text as M Âą s, where M - mean , and s - standard error of the mean.

Results and Discussion Subcutaneous injection of turpentine intact animals resulted in the first day to a sharp decline in leukopenia with absolute lymphocyte count more than 4 times , and the next time their gradual increase . The combined effect of BA and BC led to a greater reduction in these parameters (Figure 1).

In the experimental animals after 7 days after exposure to turpentine and treatment IHF -2 by reduction of the absolute lymphocyte counts the total number of white blood cells to 187% higher than the data of untreated animals. During this period a number of neutrophilic studies compared to the control was increased absolute amount of band and segmented neutrophils 2 and 1.4 times , respectively.

After 14 days of the absolute number of total leukocytes and lymphocytes experienced rats treated IHF - 2, compared with the experience without treatment rose by 71 % and 89 % , respectively, while the monthly term studies in relation to the control of these rats was observed leukocytosis due to lymphocytosis and neutrophilic leukocytosis with a nuclear shift to the left. Thus , the absolute number of lymphocytes , stab and segmented neutrophils under the influence of IHF 2 was increased by 22.4 % , 255.2 % and 141 %, respectively.

After 45 days of peripheral blood neutrophil response persisted , although the total number of leukocytes by the absolute lymphocyte count compared to the control level was lowered.

Thus, under the influence of IHF -2 in

animals with experimental inflammation of the total number of white blood cells by increasing blood lymphocytes and neutrophils recovered after 7 days and lasted for a month, after which there was a decrease the total number of white blood cells, which, in our opinion, due to the completion of the inflammatory process.

The study of the absorption and metabolic activity of neutrophils showed that on the 7th day after the subcutaneous injection of turpentine intact animals , compared to 1 for days there was an increase of spontaneous and stimulated NBT test and phagocytic activity of neutrophils ( Figure 2).

On the 14th day of the study of spontaneous and induced NBT-test performance did not differ from controls, whereas spontaneous phagocytic activity of neutrophils was reduced by 18.8 %. By 30 days of all indicators of spontaneous and induced NBTtest and the phagocytic activity of neutrophils exceeds the control value of 29.5 %, 35%, 30.7% and 40.2 % respectively.

As seen in Figure 3, the introduction of turpentine animals previously baited BA and BC, on the 7th day resulted in low levels of spontaneous and induced HCT and the phagocytic activity of neutrophils, respectively, 39.4% , 35.6 %, 20% , 41.6 %.

Such a pattern was also observed at 14 and 30 day study.

At the same time, the drug treatment the animals IHF-2 as 7 and 14 and on day 30 significantly increased rates of spontaneous and induced by NBT-test and the phagocytic activity of neutrophils that were significantly higher than in intact animals.

As can be seen from Figure 6 the level of the CEC under the influence of IHF -2 in experimental animals with experimental inflammation by day 14 of the experiment by more than 2 times the data of untreated animals after 30 days of control and reached only 13.6% slower than his 45 day experiment.

Thus , the preparation 2 IHF inflammation caused as the control animals and against toxic metal salts had a significant protective effect , as manifested in the increase in the number of leukocytes , lymphocytes, neutrophils phagocytic activity , reducing the damage to neutrophils.

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ISJM Figure 1 - The content in the peripheral blood of experimental animals with aseptic inflammation absolute lymphocyte (109 / l)

Figure 2 - Indicators of NBT test of intact animals at 1.7 days after exposure turpentine

Figure 3 - Indicators of NBT-test experimental animals with aseptic inflammation at 7 days after exposure to turpentine

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Issue 03 / November 2013 Figure 4 - Performance NBT test experimental animals with aseptic inflammation treated with IHF-2 at 7 days after exposure to turpentine

Figure 6 - The content of the CEC in rats with experimental inflammation after seeding vanadium and chromium and treatment of IHF -2

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ISJM BIOSAFETY OF A PERSON IN ELECTROMAGNETIC ENVIRONMENT Nurdaulet Zholdasov, Islamzhon Karimov E-mail: islamk97@mail.ru Scientific Supervisors: Alfiya Rafkatovna Mustafina (Doctor of Medicine, professor of KazNMU n.a. S.D. Asfendiyarov, director of Scientific-Research Institute of Fundamental Medicine n.a. B.A. Atchabarov, Almaty); Yuriy Vyacheslavovich Arkhipov (Doctor of Physical and Mathematical Sciences, professor, holder of the chair of Plasma Physics and Computer Physics of Al-Farabi Kazakh National University, Almaty) Project Supervisor: Baktygul Askerovna Kizbayeva (teacher of physics of Arystan SL in Talgar area of Almaty region) Arystan Specialized Lyceum, Talgar area of Almaty region

The Purpose of the Research The results of our investigation are to confirm or refute the known data about harmful effect of EM radiation on human body.

Hypothesis Is the known data about computer EMR and its effect on health condition of students that must be protected by state supervision from harmful effect of electromagnetic fields confirmed?

Objectives 1. To study specialized literature on the research subject.

2. To carry out an integrated survey of computer classrooms and compare it with Sanitary Regulations and Standards of RK: Đ°) to measure temperature and relative humidity of air; b) to measure parameters of computer classrooms and working places; c) to measure electromagnetic field strength near PCs and indentify hazardous computers; d) to measure radioactivity level.

3. To assess the safety degree of LCD monitors.

4. To determine the rating value of energy density.

5. To carry out a questionnaire on health condition of PC users.

Experimental Techniques 1.

Research physical Microclimate study.

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method.

2. Sanitary and hygienic method. Studying computer classroom parameters.

3. Electrometric method. Measurement of EMR intensity.

4. Dosimetric method. Measurement of radioactivity level.

5.

Sociological method.

Questioning students.

The Novelty of the Research 1.This work is a pilot project of the lyceum students.

2. First integrated survey of computer classrooms was carried out: EMF intensity, level of radioactive background, air humidity, temperature condition and parameters of working places of PC users were measured.

3. The rating value of EMF density for the Republic of Kazakhstan in the range of frequencies of computer hardware operation was established in this paper.

4. Radiations of monitors of two types were compared: monitors on the base of cathode ray tube and liquid-crystal displays.

5. Analysis of measurements and their compliance with Sanitary Regulations and Standards of RK were carried out.

Findings and Conclusions.

1. Computer is the most dangerous EMF source, as person spends a lot of time in close proximity to it. The full set of computer hardware radiates: monitor, keyboard, processor, leading-in cables.

Our measurements showed that liquid-crystal display can be recognized as the most safety device.

2. In comparison with the monitors of an older generation (monitors with cathode ray tube LGFlatron 710 TH 2003 year of manufacture) liquid-crystal displays (SamsungSyncMaster 933, 2009 year of manufacture) show radiation level by electric

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Issue 03 / November 2013 component 70 times less, by magnetic component 11 times less, by radiation energy density 1,5 times less.

3. Values of radioactive background (γrays) within the norm: for power handling capacity of exposure dose 0,30 µSv/h the measured value is 0,22 µSv/h.

4. We calculated that the rating value of energy flux density in the range of frequencies 2kHz-400kHz is 5 µW/cm2 for Kazakhstan. PC radiation norms are not determined in Sanitary Regulations and Standards of RK. 5. Questionnaire analysis showed that all PC users (students of 9-10 grades) feel various degrees of EMF effect on overall health: fatigability - 32,5%, headache 16,25%, nervousness - 10%, sleep disorder - 8,75%, smarting eyes - 46%, dizziness - 12,5%, and etc.

Practical Value 1. First EMR effect on vulnerable child’s body was demonstrated.

2. The rating value of energy flux density of computers – 5 µW/cm2, was determined for Kazakhstan.

3. Biosafety of LCD monitors was proved.

4. This paper will help doctors of educational institutions in planning of working places, PC work dosing.

5. Working materials in the research paper of students are useful for teachers of physics (computer science) to get a visual picture of EMR from the computers in use.

Proposals 1. To the Ministry of Public Health of RK: а) To develop a government program of environmental monitoring and control over the level of EM-radiation in all educational institutions (irrespective of the form of ownership). b) To decommission computers with radiation exceeding the rating values.

2. To the Ministry of Education and Science of RK: а) To introduce a mandatory briefing on planning of PC user working place, dosing of lesson hours, compliance with the norms of a positive microclimate in educational institutions. b) To include into the program of physics (computer science) school course the EMR research of computers in use as a laboratory work for early familiarization of students with the global issues of biosafety.

3. To the Ministry of Justice of RK: To develop a bill of high demands to computer hardware, when importing it to Kazakhstan: mandatory conduction of measurements of computer EMF strengths immediately before purchasing, as it is done in many countries.

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ISJM INFLUENCE OF ANTHROPOGENIC FACTORS ON HEALTH Ikramova S.E. E-mail: i.sanim02@gmail.com Supervisor: Bakirova K.Sh. (Doctor of Biological Science, prof. KAZNPU n.a. Abay) Kazakh National Medical University named after S.D. Asfendiyarov

Summary In article, considered factors influencing a human body. The problems of development are revealed and certain resolutions of these problems are given.

Keywords electromagnetic fields, nervous system, radiation, high technology XXI century provided people with innovative high technology simplifying their everyday life as well as with a danger of health issues arising from domestic electronics usage. When looking to purchase a new refrigerator, microwave stove or television, not many of us think of the consequences of using those electronics in our everyday life.

Any electronics radiate hertzian waves and are considered to be the source of the electro-smog, hiding a threat for health. [1.]

Experts believe that hearths of radiation contaminations are local, while electromagnetic radiations are ubiquitous. We are constantly exposed to the electromagnetic radiation influence.

The total tension of electromagnetic fields quickly increases as compared to natural tensions with a safe background.

Radiations, by affecting organism, weaken our immunity, and a person becomes more exposed to the infections that intensify his or her chronic diseases.

Hertzian waves influence your inprocess endocrine, nervous systems, and cause cardiac activity failures. Two potential factors of danger are the intensity of the radiation and duration of the contact with the source. But it does not mean that all domestic electronics are potentially dangerous - it simply states that we need to use it in the right way.

Scientists, studying the effects of electromagnetic radiation on our health, consider television has the most negative influence on people, if it is continuously turned on.

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The second place goes to a computer, which possesses the whole bouquet of different sources of electromagnetic radiation with high intensity. By the way, both television and computer are often equipped with network filters, sources of trouble-free feed and other ancillaries, that promote the closeness of the electromagnetic fields sharply. The third place goes to mobile telephones: their influence is directly exposed to our cerebrum that is very radiation-sensitive. Another source of strong "producer" of electro-smog is a refrigerator.

In fact, it works 24 hours without any interruptions.

A situation becomes dangerous, when few electronics are concentrated on a small area and work simultaneously. For example, when we make our dinner, microwave, refrigerator, toaster and many other kitchen supplies are in use.

[2.]

The nervous, immune, endocrine, urogenital, cardiovascular systems and organs of sight are the most vulnerable for the electronics usage. The effects may result in a loss of memory, increase of propensity to development of stress reactions, rapid fatigue ability, insomnia, and permanent colds.

Experiments showed on animals - at the protracted contact with a radiant there are serious illnesses of the nervous system, leucosisâ&#x20AC;&#x2122;s, brain neoplasms, hormonal violations. Natural defense of people are their biofield. But science did not study enough biofield that co-operates with the electromagnetic fields. However, it is exactly known that the working in zone actions of radiation grumble irritability, intolerance, in 1-3 at sense of internal tension, fussiness,

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Issue 03 / November 2013 appears some from them. Attention is violated. There are complaints about small efficiency of dream, headaches and fatigue ability.

How many it is possible to talk?

Physicians are sure: if you talk on telephone a hour one and a half in a day, get the dose of radiation, as on a harmful production. And, on logic, must every year pass medical commission. Those who speak 3 hours in a day, officially get impermissible, that dangerous dose of irradiation.

In May 2011, the WHO's International Agency for Research on Cancer published a review of the evidence on health risks of electromagnetic fields (EMFs), and concluded that cellphone users might be at increased risk of glioma and acoustic neuroma, and that there was inadequate evidence of any other health risks posed by EMF [2.]

Rules to protect yourself from the danger of the electromagnetic fields: 1. Look after the right placing of electronic devices in your house: they must be placed in a distance of more than a 1,5 m from people. It concerns not only the refrigerator and television, but also the power modules, charge devices, and telephone "bases."

2. Turn off and disconnect the devices after you finish working with them (blender, coffee-maker, iron and etc.)

3. Bedroom needs to be considered as the most ray-proof place, where people spend their 6-8 hours. Therefore, do not place television, computer and etc. over there. A wall outlet for a night-light mustnâ&#x20AC;&#x2122;t be closer than a meter to your bed.

4. All domestic electronics must be connected to the system of power supply in a correct way.

5. When expecting a connection on your cell phone, do not bring your phone to the ear, watch signals on your display.

Remember: during the call a maximum radiation arises until one picks up and honks.

During the conversation the power of radiation falls at times.

6. Carry your cell phone in a bag, not on your belt or breast. After dialing up wait till the connection is made and then bring the phone to the ear. Try to speak as short as possible, no more than 1-3 minutes after connection is made.

7. It is not recommended for expectant mothers to use a cell phones. Embryos are ultrasensitive to the waves; also, a whole body of embryo is exposed to the rays.

8. It is better to disconnect your phone at night. If it is impossible, keep your phone in the distance of minimum 1,2 m from your head.

9. Eliminate the usage of telephone by children. If a child plays with the phone, put it in the mode of "flight", because that mode does not allow the phone to radiate.

10. According to the Kazakh norms, working with the computer should take no longer than 4 hours per day with an interruption through every hour of your work.

11. The farther you are from an electronic device, the safer you are: move aside from a monitor about 70 centimeters and try to put the keyboard from the monitor for a half meter.

12. If you live in a private sector or on the ground floor, put in earth metallic pin and from a computer, through the window, stretch a copper wire to it.

13. Wipe your monitor, keyboard and desktop by a moist (not wet) rag before using it. Thus, an electrostatic charge will be taken off.

14. When you warm up your meal in a microwave, do not wait right close to it, better to step back a little.

15. If it is possible, purchase the special protective devices for the individual use (Global Industrier Ltd company is the main producer of it). These devices in a radius from one to three meters purge the field from super harmful vortical currents. Signals from these devices are clean and practically harmless for an organism.

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ISJM Device, itself, is provided with a calendar or ticket-sized special informative matrix, which transforms an incoming wave and at the same time protects from an external radiation. It’s both polarity and rotation changes and radiation is neutralized. A feed for this device is not required. Simply, you have to carry the charger with itself or, for example, fasten on a cell phone. [3.]

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Literature: 1.

World Health Organization. WHO definition of Health, Preamble to the Constitution of the World Health Organization as adopted by the International Health Conference, New York, 19–22 June 1946; signed on 22 July 1946 by the representatives of 61 States (Official Records of the World Health Organization, no. 2, p. 100) and entered into force on 7 April 1948.

In Grad, Frank P. (2002). "The Preamble of the Constitution of the World Health Organization". Bulletin of the World Health Organization 80 (12): 982.

2.

World Health Organization. 2006. Constitution of the World Health Organization – Basic Documents, Forty-fifth edition, Supplement, October 2006.

3.

UNESCO. The UN World Water Development Report: Facts and Figures – Meeting basic needs. Accessed 12 May 2011.

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Issue 03 / November 2013 THE ANALYSIS OF CAUSES OF THE DIVERGENCE OF CLINICAL AND PATHOANATOMICAL DIAGNOSES BASED ON THE RESULTS OF OPERATIONAL AND SECTIONAL MATERIALS Abdimanap A., Momynov B. E-mail: dubsomnia@gmail.com Department of Pathological Anatomy Kazakh National Medical University named after S.D. Asfendiyarov

The study contained the data of 97 patients with stroke, according to the hospital of emergency urgent care for 2013 during the first 3 days from the onset of illness. The type of stroke in all cases was verified by the results of an MRI or CT scan, and in the case of death - as the results of pathoanatomical research. Also defined the frequency of diagnostic errors with cerebral infarction, intracerebral hematoma and subarachnoid hemorrhage.

The total number of the diagnostic errors of stroke revealed seven cases, which amounted to 7.22%. It was concluded that the modern diagnosis of stroke and determination of indications for neurosurgical treatment impossible without CT in the first hours after the disease and MRI in the next 2-3 days. Low quality of medical services - is a belated diagnoses, unnecessary surgery, inappropriate therapy and unjustifiable increase in costs. The proper quality of medical aid - is conformity of provided medical care to modern concepts of its required level and volume for a given type of pathology taking into account individual features of patient and capabilities of a particular medical facility (V.S. Luchkevich.1997).

In addition to, there were conducted the studies byoperational material and revealed 35 cases of the divergences of clinical and pathohistological diagnoses from 21250 cases which is amounted to 0.16%of the total number of patients according to the hospital of emergency urgent care for 2012-2013. From 35 cases was established 25 cases of cancer representing 77% of the total number of discrepancies and 8 cases of nontumorous (inflammatory) disease, which is 23% of the total divergence.

Differential diagnosis of stroke character only on the grounds of clinical data is not

possible, wherein the number of errors in hemorrhagic stroke is higher than in ischemic stroke. Immediate enactment of CT or MRI allows in the next few hours after the onset of stroke to determine its type (ischemia or hemorrhage), on top of that initiate a differentiated therapy and, where appropriate, urgently bring a neurosurgeon for further conducting the patient. Previously it was thought that the most promising techniques that allow to recognize the type of stroke with a high degree of reliability are echoencephalography and cerebrospinal fluid studies. Unfortunately, these methods are not always informative, especially in ischemic stroke.

Gender and age structure: Age ranged from 41 to 71 and older. Nearly two-thirds of patients at the time of death were in the age group over 60 years. Gender of patients 56% (4) men, 44% (3) women. Average age of patients is 65 years. Considering this and based on the clinical and pathoanatomical diagnoses all 7 cases were classified toII category of divergences (in this medical facility a correct diagnosis was possible, however, diagnostic error generated by subjective reasons does not significantly affect the outcome of the disease).

In the hospital of emergency urgent care of Almaty city for 2012 hospitalized 1089 patients. From them patients with ischemic stroke were 65%, intracerebral hemorrhage 24.5%, subarachnoid hemorrhage - 3.5%.

Overall mortality rate was 13.6%, mortality at an ischemic stroke - 10.87%, intracerebral hemorrhage 23.2%, subarachnoid hemorrhage â&#x20AC;&#x201C; 13,1%. The percentage of divergence of the diagnoses of stroke was 7.45% (Analysis of mortality in patients with cerebral stroke in Almalinsky and Bostandyksky district of Almaty city for 2012.

Bekbaev B.A. City Hospital of emergency urgent care of Almaty city.).

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ISJM The aim of this study To compare the coincidence of diagnoses on admission with the final diagnosis, as well as to determine the frequency and causes of misdiagnosis of the type of stroke, based on the initial assessment of clinical symptoms.

The age of patients mostly of an advanced age or senile age. Adulthood-2 cases, the advanced age - 3 cases and senile age - 2 cases. Considering this also on the grounds of clinical and pathoanatomical diagnoses all seven cases were classified to II category of divergences (in this medical facility a correct diagnosis was possible, however, a diagnostic error generated by subjective reasons does not significantly affect the outcome of the disease).

Most physicians trained in clinical internship, but still do not have the qualification category.

Available methods of examination at the admission department in the evening hours and weekends is only cerebroscopy ultrasound.

High qualification and extensive experience of physicians of the neurological and an intensive care department do not always allow to completely avoid mistakes in the diagnosis of stroke character, which once again proves the necessity of emergency neuroimaging at hospitalization of patients with acute disorders of cerebral circulation.

Performed analysis explains the reasons for misdiagnosis of character of stroke, especially in the early stages. Erroneous diagnosis of stroke is caused by thevariability of clinical symptoms, especially in the first hours of its development, the absence of signs of severe intracranial hypertension and meningeal syndrome with intracerebral hemorrhages.

In analyzing of operational material were taken the cases when malignant tumors had been detected. Adenocarcinoma -11 cases, desmoidfibromatosis -1 case and stromal sarcoma - 1 case.

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If we take adenocarcinoma, they were mainly localized in the organs of gastrointestinal tract (stomach and intestines). Malignant tumors have polymorphism of clinical manifestations, which is often the cause of serious diagnostic errors.1) initial tumor growth occurs with a picture of "inflammatory" changes in the tissues, causing erroneous diagnoses 2) malignancy in the area of long-existing chronic pyoinflammatory processes.

According to the literature [Logunov K.V. - The choice of treatment of gastric ulcers: Author. dis. M.D. SPb., 1999.- p-46. Popovich A.U. Treatment of locally advanced gastric cancer / / International Medical journal. - 1998. - № 1.

-Pp. 104-106.]at the time of determining the indications for operative intervention aboutchronic ulcer in 35-40% of patients diagnosed with cancer, and about 70% of all gastric tumors detected on the background of peptic ulcer.

Accordingto the city pathoanatomical bureau in St. Petersburg for 1993-1997 discrepancies of intravital and postmortem diagnoses were 18% of the total number of deaths, suffering from peptic ulcer disease and gastric cancer.

Difficulties in detecting adenocarcinoma Causes: • The complexity of visual differential diagnoses between early forms of cancer and benign changes; • Underqualification of doctors; • Using of imperfect equipment; • Absence of criterion for creating a risk groups in the population.

Conclusion The analysis of sectional and operational material showed that: 1) Vivo diagnosis of stroke presents certain difficulties, due to not completely examine of patients in the older age categories.

2) The percentage of the discrepancies of clinical and pathoanatomical diagnoses by operational material is not high and associated with the complexities of diagnosing tumors and requires oncological alertness.

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Issue 03 / November 2013 The table of the divergences of diagnoses by sectional material for 2013 The day of an autopsy

Gend er

Age

Departm ent

The dayofadmi ssion 12.01.13 1225

The dayofdisch arge 19.01.13 645

19.01.13

M

64

Therapy

11.02.13

W

45

17.05.13

M

17.06.13

Clinical diagnosis

Pathoanatomicaldia gnosis

Catego ry

Cerebrovascular disease. Acute cerebrovascular accident,hemorrhag ic type in the right frontal lobe.

Cerebrovascular disease. Acute cerebrovascular accident, hemorrhagic type with a breakthrough in the ventricles.

Cerebrovascular disease.Acute cerebrovascular accident,ischemic type in the brainstem area 4x3sm. Erosion of the ventricles in the cardiac department.

Hemorrhage in the pancreas Cerebrovascular disease. Acute cerebrovascular accident, ischemic type inthe left.

II

NSD (NeuroSomatic Departm ent)

14.02.13

17.02.13 825

Cerebrovascular disease.

Acutecerebrovascul araccident.

Hemorrhagic stroke with hemorrhage into the brain ventricles. Brain edema.

73

NSD

10.05.13 1950

17.05.13 1000

M

82

NSD

15.06.13 0707

16.06.13 705

Cerebrovascular disease. Acute cerebrovascular accident, hemorrhagic type with a breakthrough in the ventricles.Brain edema.

Cerebrovascular disease. Acute cerebrovascular accident, ischemic type. Brain edema.

Cerebrovascular disease. Acute cerebrovascular accident, hemorrhagic type in the right frontal lobe.

Cerebrovascular disease. Acute cerebrovascular accident, ischemic type in the left temporal region Cerebrovascular disease. Acute cerebrovascular accident, hemorrhagic type.

II

02.08.13 1115

W

76

NSD

01.08.13 0945

01.08.13 2130

Cerebrovascular disease. Acute cerebrovascular accident, hemorrhagic type.

28.08.13

M

41

Neurolog ical dept.

25.08.13 1137

27.08.13 2200

09.09.13

W

75

NSD

01.09.13

07.09.13

Cerebrovascular disease. Acute cerebrovascular accident, ischemic typein the vertebrobasilar basin.

Cerebrovascular disease. Acute cerebrovascular accident, ischemic type. Left-sided hemiplegia. Arterial hypertension.Chron ic cholecystitis.

Ischemic heart disease.

Cardiosclerosis.Cilia ry arrhythmia.

Brain edema

Cerebrovascular disease. Acute cerebrovascular accident, ischemic type. with hemorrhagic impregnation in temporoparietal area

II

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II

II

II

II

ISJM The table of the divergence of diagnoses by operational material for 2012 The date of material receiving 19.01.12

Gen der

Department

M

Surgical dept.

31.01.12

W

06.04.12 20.04.12

W M

gynecological dept.

Surgical dept.

Surgical dept.

26.05.12

M

Urology

04.06.12

W

Surgical dept.

Pepticulcer.

Gastrichemorrhage

18.08.12 12.10.12

W M

Surgical dept.

Urology

- ?

Benignprostatichyper plasia

24.10.12

M

Urology

Benignprostatichyper plasia

03.11.12

W

Surgical dept.

14.11.12

W

Paid dept.

08.02.12

W

30.04.12

W

Departmentof Infectioussur gery gynecological dept.

07.05.12

W

Surgical dept.

16.05.12

W

gynecological dept.

30

Clinical diagnosis

Pathoanatomicalconclusion

Age

Cavernous hemangioma of the liver, C-r Hysteromyoma.

Metrorrhagia Fibroma? Sarcoma?

Fibroma of the left foot Abscessed orchiepididymitis in the right

Express biopsy: hepatocellular carcinoma G2 medium differentiated Clear-cell adenocarcinoma of the endometrium Angiofibroma Angiofibroma

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Tuberculousorchiepididymitis with secondary purulent inflammation. Phthisiatricianâ&#x20AC;&#x2122;s consultationisnecessary Moderately differentiated adenocarcinoma of the stomach G2with tumor cell invasion up to the serosa surrounding adipose tissue. In the studied edges of resection the elements of tumor werenâ&#x20AC;&#x2122;t found.

Adenocarcinoma of the stomach Adenocarcinoma of the prostate gland.GleasonGradingsystem4+ 3=7 Adenocarcinoma of the prostate gland.GleasonGradingsystem4+ 3=7 Gastric carcinoid with histological signs of malignancy Adenocarcinoma of the fallopian tube Intestinal adenocarcinoma with invasion to the medial shell.

Withsecondaryulceration Uterine leiomyoma. Glandular hyperplasia of the endometrium.

Endometriosis of the uterus 1 degree Metastasis of ovarian adenocarcinoma with clear cell sites in the wall of the ileum Endometrioid endometrial adenocarcinoma G2 secretory villus 0.7 cm growing into the myometrium.

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Pepticulcer Infertility.Doubleside dpiosalpingit Acuteobstructiveinte stinalocclusion Laparoscopy of subtotal hysterectomy without appendages AHF. Adhesivedisease Uterine myoma.

Endometrial cyst of the right

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46 32 42

77

60 73 78 72 38 84 47

38 62

Issue 03 / November 2013 The table of the divergence of diagnoses by operational material for 2013 The date of material receiving 10.01.13 11.01.13

Gen der

Department

W W

Surgical dept. gynecologicalde pt.

Surgical dept.

16.01.13

M

16.01.13

M

Endoscopic dept.

02.02.13

W

09.03.13

M

Paid gynecologicalde pt.

Urology

27.03.13

M

16.07.13

M

27.05.13

M

19.06.13

M

DepartmentofIn fectioussurgery Paid Endoscopic dept.

DepartmentofIn fectioussurgery Surgical dept.

01.08.13

M

Urology

05.08.13

M

DepartmentofIn fectioussurgery

06.08.13

W

DepartmentofIn fectioussurgery

06.08.13

M

12.08.13

W

14.08.13

M

DepartmentofIn fectioussurgery DepartmentofIn fectioussurgery Urology

16.08.13

W

DepartmentofIn fectioussurgery

17.08.13

M

Urology

22.08.13

W

gynecologicalde pt.

Clinical diagnosis Sigmarectal C-r Bleedinginmenopause Perforation of the small intestine. Diffuse, fibrinousperitonitis Erosive hemorrhagic gastritis. Ulcer of the stomach angle Bleedingduringpregnanc y Vesiculitis. Hyperfusion of prostate seed tubercle Papilloma Ulcer of of the gastric cardia Anteriorabdominalwalla bscess Cancer of the small intestine Benignprostatichyperpla sia C-r of sigmarectal part of the colonT4 Nx Mo. diffusive C-r of sigmarectal part of the colon, complicated by obstructive ileus, with perforationT4 N1Mx Papillomaofback Ligature abscess of the anterior abdominal wall Sclerosis of the bladder neck Colontumor Benignprostatichyperpla sia Hysteromyoma.

Metrorrhagia

Pathoanatomicalconclusion

Age

Fibropurulentperitonitis Moderatelydifferentiatedendometri aladenocarcinoma Stromal sarcoma of the small intestine with the tumor necrosis

77 55 54

Moderately differentiated adenocarcinoma of the stomach

77

Squamous cell carcinoma of the cervix

49

Chronic cystitis with squamous metaplasia of the hearth Seborrheic necrosis with chronic inflammation in the acute stage Gastricadenocarcinoma

26

Desmoidfibromatosis

82

Necrotizing ulcerative focal productive enteritis, Crohn's disease is not excluded Adenocarcinoma of the prostate gland.Gleason Grading system4+4=8 Intestinal polyposis. Chronic erosive colitis with inflammation

53

51 71

72 44

Chronic polyp with hyperplasia of glands

66

Intradermal nevus with secondary inflammation Metastasis of adenocarcinoma with secondary inflammation Nodalstromalprostatichyperplasia

20

Chronic enteritis, with hyperplasia of lymphoid follicles, proliferation of glands Adenocarcinoma of the prostate gland.GleasonGradingsystem4+3= 7 Medium-differentiated adenocarcinoma of the uterine body G2

75

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78 45

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ISJM ASSESSMENT OF ELECTROPHYSIOLOGICAL PROPERTIES OF THE MYOCARDIUM IN ALMATY METRO WORKERS Isamatov B., Lee E., Sultanova A., Kulmaganbetov M., Umithan B. E-mail: beka_1502@mail.ru Module “Gerontology and Geriatrics” Kazakh National Medical University named after S.D. Asfendiyarov

Actuality Currently urgent problem is early detection of the risk of socially significant diseases of the cardiovascular system.

One of the most effective measures to solve this problem are mass screening of the population, so there is a need to implement into the medical practice contemporary screening technologies that allow promptly identify those, who has a high risk of developing diseases of the cardiovascular system.

Purpose of work Assessment of electrophysiological properties of the myocardium in healthy metro workers of Almaty city in different age groups for the early detection of the risk of cardiovascular disorders.

Materials and methods Screening studies were conducted among workers of different professional groups of underground staff. Surveyed 492 adult workers, including 333 women and 159 men. Average age of the examinees was 35,82 ± 0,57 years (from 20 to 66 years), of which the average age of women 41,35 ± 0,88 years, men 35,82 ± 0,57 years. Cardiovascular system was evaluated using six-channel electrocardiography (ECG) with registration in 12 conventional leads and device data “Screening system of the heart “Cardiovisor 12C” with a software package “KARDi-2” based on the method of dispersion charting electrocardiogram (ECG DC). DC ECG method - this is one of the new methods used for screening in Cardiology for the early detection of violations of electrophysiological properties of the myocardium according

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microalternations of cardiocycle that are not detected by conventional ECG. The method is based on the formation of informationtopological model of small oscillations ECG electrical microalternations of ECG signal.

Basis microalternations of ECG signal account a violation of the ion transport function, structure of cell membranes and mitochondrial energy production, microcirculation disorders and several other factors. Deviation of wide variety of electrophysiological characteristics under various pathological processes leads to a change in the amplitude of microalternations. The nature and degree of change of microalternations is a new diagnostic of area of signs that reflect the "stock" of electrophysiological compensatory resources of myocardium [1, 2, 3, 4].

Therefore, this new method is used in clinical cardiology to identify adaptive responses and early disorders of the electrophysiological properties of myocardium, i.e. as effective diagnostic markers upcoming restructuring.

Numerical expression of the low-amplitude oscillations dispersion analysis in time intervals PQRST complex is reflected in the integral indicator "Myocardium", which at less than 15% indicates normal spread of values at 15-27% - probability of heart disease and the need for a comprehensive differential diagnostic survey, a value more than 27% - pathological disorders in the heart and the need for a special in-depth survey.

To enhance the diagnostic capabilities of the methodology was used exercise stress test (20 squats) with the assessment of recovery after 5 minutes.

Statistical data processing was carried out in the program «SPSS».

Results and discussion The surveyed were grouped into the following age groups according to the WHO classification of age:

Issue 03 / November 2013 | isjm.kaznmu.kz

Issue 03 / November 2013 Group 1 - less than 24 years (9,8 ± 1,34%), Group 2 - 25-44 years (60 ± 2,21% ), the third group - 45-59 years (28,4 ± 2,03%), group 4 - 60 years and older (1,8 ± 0,6%). It is revealed that with age there is an increase values of "Myocardium". Statistically there is significantly different levels of “Myocardium” in the 1st and 4th age group, p ≤ 0,05 at rest and there was a regular increase in the parameter “Myocardium” in all age groups after physical activity (Table 1).

Interesting is the following - in a group of healthy young adults were identified slow return "Myocardium" to baseline values. There is revealed a linear relationship between "Myocardium" and indexes of details G7 and G9 (p <0.01), reflecting the symmetry of ventricular depolarization and compensatory reactions of the left ventricle, respectively. It is believed that the most dynamic indicator of the compensatory mechanisms of the left ventricle by DC EKG is G9. According to the interpretation of changes in this indicator small values - G9 <5 may be due to the individual characteristics of myocardium, and the values G9> 5 are predictor of hidden abnormalities.

To detect hidden abnormalities analyzed the selected representative DC EKG indicators reflecting insufficient oxygenation of myocardium or violations of myocardial perfusion. The analysis showed a statistically significant increase of them among healthy older people: G3 (p = 0,04), G7 (p = 0,02). This may indicate a decrease in compensatory mechanisms of ventricular myocardium with age and the presence of hidden abnormalities.

conventional electrocardiogram, but carry the risk of heart disease.

The practical aspect of the research is that the obtained evidence-based data about the successful use for screening tests in mass screening - DC ECG via the device "Cardiovisor-12C." Technology differs methodological simplicity execution, highly informative and capacity for mass surveys.

The State Program on Development of Health "Salamatty Kazakhstan" for 20112015 provides for the implementation of priority on building effective health care system. As ways of the achieving the objectives defined direction for the improvement and development of its preventive orientation, based on the development of an effective system to identify and assess the factors that cause the development of diseases, strengthening preventive measures and screening studies [5]. Therefore, our study is consistent with efforts to implement the State program "Salamatty Kazakstan", in particular with the provisions of paragraph 5.2 - "Strengthening of preventive measures, screening tests, improving diagnosis, treatment and rehabilitation of basic social diseases and injuries."

References 1. Using the appliance 'CardioVisor-06c "for screening.

Guidance for doctors. - M. - 2004. - 238. p.

2. Ivanov G.G., Sula A.S. ECG dispersion charting method in clinical practice. Moscow, 2008 3. Baevsky R.M., Berseneva A.P. Correlation of heart rate variability with indices of ECG dispersion charting in different functional states of the organism. / / Proceedings of the IX Congress of the Russian Society of Holter monitoring and noninvasive electrophysiology. Suzdal. 2008. P.31.

4. Sula A.S., Ryabykina G.V., Grishin V.G. ECG - analyzer CardioVisor-06c, new opportunities for identifying myocardial ischemia during screening examinations and prospects in functional diagnostics / / "Functional diagnostics" № 2, 2003 - S. 93-100.

5. State Program for Development of Health "Salamatty Kazakhstan" for 2011-2015

Conclusions These changes reflect a decline of compensatory reactions with age and early violations of electrophysiological properties of the myocardium, i.e. impending restructuring in the 4th age group that do not appear on the

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ISJM Table 1. Data in terms of "Myocardium" in the age groups Age groups

“Myocardium” at rest

“Myocardium” after

“Myocardium” after 5 minutes

physical activity

of recovery

1 group

15,35±0,63

19,40±1,46*

17,33±1,13#

2 group

14,77±0,34

18,88±0,48*

15,96±0,40#

3 group

15,40±0,49

18,91±0,69*

15,90±0,42#

4 group

17,44±2,34

21,22±3,44*

16,44±1,32#

Note: * - significant differences in comparison with “at rest”; # - significant differences in comparison with “after physical activity”

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Issue 03 / November 2013 | isjm.kaznmu.kz

Issue 03 / November 2013 CLINICAL AND LABORATORY FEATURES AND ANTIBACTERIAL THERAPY OF LISTERIOSIS IN CHILDREN AT THE CURRENT STAGE Dzharbolova A.N. Mazhibaeva A.K. Nurgayn N.N. Kenenbaeva L.A. Zhumabai R. E-mails: akerke.dzharbolova@mail.ru, akbota_m@mail.ru Supervisor: Onalbaeva G.Zh. Kazakh National Medical University named after S.D. Asfendiyarov

Keywords: Listeria, clinic, antibiotic therapy Materials and Methods • In a scientific research there are examined 53 case histories of children who received treatment with a diagnosis of listeriosis in CCCIH (City Clinical Children Infectious Hospital) within one year (2012-2013).

• Out of all children there suffered from recurrent type of listeriosis 2 children.

• Diem was on average 9 days.

• In most cases of listeriosis was leaking on anginal- septic type in 87% (46) and eye-iron type 13% (7).

Results and conclusions of the study In our study listeriosis was encountered all year round and sexual characteristics were not observed: boys – 51% (27) and girls – 49% (26) had listeriosis equally common.

In the age structure of young children (1-3 years) 41.5% (22), preschool age (3-7 years) 28.3% (15) and school age (7 years) 26.4% (14) were ill equally common. And in children under 1 year, the incidence of listeriosis was 3.7% (2).

In the form of listeriosis monoinfections encountered in 68% (36) of the cases. 36% (17) of children were ill mixed infection and always proceeded with yersiniosis.

The disease often causes a severe form 66% (35). In clinical polymorphism often met poliadenopatiya 75% (40), catarrhal phenomena (in the form of hyperemia of oropharynx, cough, runny nose) 64% (34) and an increase in body temperature to febrile and high numbers of 41% (20) and which has lasted more than 1 week 55% (29).

Joint pain in 11% of listeriosis (6), rash 30% (16) and vomiting 19% (10), diarrhea 21% (11), abdominal pain 17% (9) were rare.

The disease occurs with hepatomegaly in 32% (17). Articular syndrome is often observed in children of preschool age 9% (5).

Indicators of hemogram with listeriosis neytrofillez 34% (18) and lymphocytosis of 38% (20) occurred in the same percentage.

And increased erythrocyte sedimentation rate was observed in most cases 62% (33).

Liver biochemical parameters with changes occur as hyperenzymemia (ALT + AST) and hyperbilirubinemia in 33% (17) cases, increasing thymol test in 20% (11).

When substantiating the diagnosis it is most often used method of ELISA was 73% (37).

When the initial antibiotic therapy of listeriosis in 65% (34) of the cases did not change and the children were discharged with improvement. As initial therapy drugs used aminoglycosides of III generation and cephalosporins of II, III generation.

Recommendations 1. When substantiating the diagnosis of listeriosis use serologic method must be mentioned in the order together with the bacteriological method.

2.

Starting therapy start with aminoglycosides of III generation (merkatsin, amikacin).

3. Active conduct health education among the inhabitants.

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ISJM INFLUENCE OF NORMOBARIC HYPOXIA ON METAL INDUCED DAMAGE OF BEHAVIORAL REACTIONS OF RATS Kulmaganbetov M.A. E-mail: m.kulmaganbetov@kaznmu.kz Supervisor: Professor Nurmukhambetov A.N., Zhukesheva M.K., Department of Pathological Physiology Kazakh National Medical University named after S.D. Asfendiyarov

Abstract In the paper there was studied the influence of hypoxytherapy to behavioral reaction of adult rats, who were harassed by lead and chromium. There was established that under the influence of heavy metal compounds inhibited the behavioral reactions of rats, whereas hypoxytherapy weakened the depressive and anxiety disorder of rats, increased the horizontal motor activity, completely prevented disorders of innate avoidance of bright light and reconstruct the process of memorizing under the reaction of passive avoidance (RPA).

Keywords: heavy metals, behavioral reactions, rats, hypoxytherapy, normobaric hypoxia

Actuality The basic sources of air pollution are emissions from the non-ferrous metallurgy, thermal power, steel, oil and gas and transportation. Threats from air pollution by heavy metals affect the deterioration of public health and environmental degradation [1-3].

Recently, in contemporary medicine there are increasingly growing interest in natural prevention, treatment and rehabilitation. Adequately matched nondrug exposure correction contribute to the optimization of the adaptation process, due to modifying the systematic organization of functions in the integrated functional system. Hypoxic condition underlies virtually any type of adaptation, and adaptation of the organism is the foundation upon which emerge and develop specific adaptations to a particular factor or group of factors [4, 5].

Purpose of the study To investigate the neurotoxic effects of lead and chromium and pathogenetic correction of disorders with using normobaric hypoxia.

Materials and methods Work carried out as part the international project “Studying of hemototoxic influence of heavy metals and possibility of activation of protective

36

and adaptive mechanisms of the organism using the normal and hypobaric hypoxia in adult and old animals in comparison” (supervisor prof. Akanov A.A.) with the financial support of Ministry of Education and Science of the Republic of Kazakhstan.

Experiments were carried out on 30 white male rats weighing 180-220 g, were kept under standard vivarium conditions on a normal diet. Conducted 3 series of experiments: 1 series - control animals; 2 series - adult rats exposed to lead acetate (LA) and potassium dichromate (PD); 3 series - adult rats exposed to LA and PD and normobaric hypoxia (hypoxia) in the each series was 10 animals. Lead acetate was administered orally at a dose of 15 mg / kg bw, potassium dichromate - 1 mg / kg bw within three weeks. Control rats were administered 0.9% solution of NaCl. At the beginning of the second week of administration of heavy metal salts rats were exposed to normobaric hypoxia within 21 days of exposure for one hour. Normobaric hypoxia model created using the hypoxicator, dispensing flow of air with 10% oxygen content. For this one hose of hypoxicator attached to the nitrogen bottle, another one attached to the second compartment (with output ventilation hole) where the experimental animals were placed. Volume of a box has been specially designed for the number of rats placed. Study of behavioral reactions of experimental rats was carried out in the test “open field” in the water maze,

Issue 03 / November 2013 | isjm.kaznmu.kz

Issue 03 / November 2013 as well as by CRAA and CRPA. Young rats served as controls, received an equivalent volume of saline NaCl. Slaughtering of animals was anesthetized (chloroform). The studies were conducted in compliance with the rules and regulations of experiments involving animals (decision of LEC of S.D. Asfendiyarov KazNMU, Protocol # 2 of 18.04.2013 y.).

Statistical analysis procedures was provided by the SPSS-16 SPT. Value of level of statistical significance was accepted for p ≤ 0,05.

Results and discussion Previous experimental studies have shown that under the influence of heavy metals in the experimental rats was growing sense of anxiety and emotional disturbance, inhibits locomotion, research and orienting activity. Under the influence of hypoxytherapy there were observed significant changes of behavioral repertoire of experimental rats (Table 1).

Under the influence of LA and PD latention of first moving in the first and second experimental sessions increased to 3 or more times (p = 0.034), under the influence of hypoxytherapy period in both studies returned to the control level.

From the presented in Table 2 results of studies show that the number of external squares crossed by experienced rats with hypoxytherapy was statistically significantly greater in 2 and more times as compared with the uncorrected experienced ones in both the first and second experimental session (p = 0.007 and p = 0.011), whereas the vertical locomotor activity hypoxic rats significantly decreased.

As shown in Figure 1, in the first experimental session, the experimental rats with hypoxytherapy “Freezing time” in comparison with uncorrected experimental rats significantly decreased to 11.9 times (p = 0,008, z = -2,667).

In the second experimental session, the rats practically did not fall into a state of complete immobility, indicating an absence of a sense of fear and danger.

From the data presented in Table 3, it follows that hypoxytherapy of experienced rats exposed to LA and PD, completely prevented the disorder of the innate avoidance of bright light and reconstruct the process of consolidation and reproducing memory engrams. Thus, in the first experimental session time spent in lighted compartment was at 194.4% significantly less (p = 0,028, z = -2,192), than the uncorrected test animals.

In the second experimental session through a memory formed from a previous session “hypoxytherapy rats” remained as controls and the rat in the bright compartment, whereas rats without hypoxic training already in 163 seconds of testing went to “the punishable compartment”.

Results of testing in the water maze showed that the experimental rats with hypoxytherapy during the elaboration time spent searching for a secure platform, was more than 2 times lower than that of the control and uncorrected rats. During verification under the influence of hypoxic training experienced rat’s seek time of platform statistically significant 4.5-times (p = 0,015, z = -2,429) ahead of the experimental rats not exposed to hypoxic preconditioning. Hypoxytherapy of rats exposed to heavy metals, had a significant influence on the spatial memory on external landmarks in both the first and second experimental sessions.

Conclusion Thus, the experimental data show that in animals treated with heavy metals, hypoxytherapy weakened the depressive anxiety, increased the horizontal motor activity, completely prevented disorders of innate avoidance of bright light and reconstruct the process of memorizing under the reaction of passive avoidance (RPA).

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ISJM 4. Fan zhong-xue, Dai Hong-xing, Bai Ai-mei. Huanjing yu jiankang zazhi=J. Environ. And health. 2007. 24, №10, p.802-803.

5. Zakharov G.A., Gorokhova G.I. Ubasheva Ch.A. Genesis of cardiac necrosis in the conditions of midlands and highlands. Bishkek, 2010. – p.207.

6. Pshennikova M.G., Popkova E.V., Pokidyshev D.A. et al.

Influence of adaptation to hypoxia on resistance to neurodegenerative brain damage in rats of different genetic lines / / Bulletin of the Russian Academy of Medical Sciences. - 2007, № 2. p.50-55 7. Dongdong Li, Tao Bai, James R. Brorson. Adaptation to moderate hypoxia protects cortical neurons against ischemia– reperfusion injury and excitotoxicity independently of HIF-1α // Experimental Neurology. -2011. –Vol. 230, Issue 2. – p.302-310

References 1.

Severine A.E., Mankaeva O.V. Ecological state of the environment and human functional reserves / / Vestn. of Kaluga Univ. 2007, № 1, p.74-83.

2.

Morozova N., Stepanova A.M. Environmental and demographic problems of health / / Kemer. Reg. Univ., Kemerevo, 2008, p.14 3.

Pertseva T.G., Nikiforova V.A., Kolesnikova O.A. Adaptation problems of older persons in the territory of technogenic pollution / / 10 Interuniversity scientific and methodological conference "Organization and methods of the educational process, sports and recreation activities," dedicated to the 75th anniversary of the Department of Physical Education and Sport, Moscow State University, 2008: Proceedings of the International Conference. M. 2008, p.303.

Table 1. - Distribution of average index of behavioral act “latent period of exit” in the control animals and animals exposed to heavy metals and normobaric hypoxia Index of behavioral act

Control Ме

Latent exit

period

of

1,5

25%-75% 1,0-4,5

Metal Ме

25%-75% Elaboration 4,8 2,0-7,0

Metal + hypoxia Ме

25%-75%

1,0

1,0-3,0

Verification Latent period of 2,0 2,0-3,0 6,0 4,0-7,0 1,0 1,0-3,0 exit Note: **** - Wilkoxon 2 –Sample Test statistical significance in relation to the metal

Wilkoxon 2 – Sample Test 0,034**** Z=-2,124 0,034**** Z=-2,116

Table 2. - Distribution of the average of the behavioral acts that characterize the horizontal and vertical locomotor activity in control animals and animals exposed to heavy metals and normobaric hypoxia Index of behavioral act

Control Ме

25%-75%

No. of outdoor squares No. of reliance on the wall

16,0

Duration of reliance on wall

Metal + hypoxia Ме

25%-75%

7,2-16,0

Ме 25%-75% Elaboration 6,0 4,0-8,0

12,0

10,0-13,0

12,5

7,5-18,8

4,0

3,0-6,5

1,0

0-2,5

25,5

7,0-52,0

8,0

5,0-13,0

3,0

0-6,0

10,2-25,5

Verification 5,0 4,5-9,5

12,0

11,5-15,0

No. of outdoor squares No. of reliance on the wall Duration of reliance on wall Note: ** - Wilkoxon 2 metal

38

Metal

17,0

Wilkoxon 2 – Sample Test 0,007**** z=-2,677 0,012** z=-2,524 0,024**** z=-2,257 0,025** z=-2,240

0,011**** z =-2,554 8,5 7,2-11,5 4,0 2,0-6,0 1,0 0-1,5 0,020**** z =-2,320 6,0 2,5-16,2 6,0 4,5-11,5 2,0 0-3,0 0,044**** z =-2,018 –Sample Test statistical significance in relation to the control, **** - in relation to the

Issue 03 / November 2013 | isjm.kaznmu.kz

Issue 03 / November 2013 Figure 1. - Distribution of average index of behavioral act “Freezing Time” in the control animals and the animals exposed to heavy metals and normobaric hypoxia 250 200 Control

150

Metal

100

Metal + hypoxia

50 0 Elaboration

Verification

Table 3. - Distribution of the average RPA in the control animals and animals exposed to heavy metals and normobaric hypoxia Index of RPA

Control

Metal

Metal + hypoxia

Elaboration

Ме 13,5

25%-75% 6,5-18,8

Ме 26,5

25%-75% 18,5-99,0

Ме 9,0

25%-75% 7,5-28,5

Verification

300

240,0-300,0

163,0

76,0-300,0

300,0

22,5-300,0

Wilkoxon 2 – Sample Test 0,028**** z=-2,192 0,018* z=-2,371 0,753****

Note: * - Wilkoxon 2 –Sample Test statistical significance in relation to the elaboration, **- in relation to the control, **** - in relation to the metal

Table 4. - Distribution of the average of the water maze in the control animals and animals exposed to heavy metals and normobaric hypoxia Index of RPA Elaboration Verification

Control Ме 46,5 6,5

Metal

25%-75% 23,2-51,0 4,2-14,2

Ме 45,5 58,5

25%-75% 24,5-76,0 20,0-88,5

Metal + hypoxia Ме 20,0 13,0

25%-75% 12,0-44,5 7,5-32,5

Wilkoxon 2 – Sample Test 0,214**** 0,015**** z=-2,429

Note: **** - Wilkoxon 2 –Sample Test statistical significance in relation to the metal

isjm.kaznmu.kz | Issue 03 / November 2013

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Dear colleagues!

We invite you to take part in the X International Multidisciplinary Congress "Neuroscience for Medicine and Psychology”. The Congress will take place in Sudak, Crimea, Ukraine from June 2 till June 12, 2014.

All information is on the Web-site http://brainres.ru (Russian and English versions).

Yours faithfully, Organizing Committee Elena Loseva, D.Sc., Nadezda Loginova, Ph.D. Institute of Higher Nervous Activity and Neurophysiology RAS, Moscow, Russia

Conference dedicated to “University Days-2013” Key Speakers

Professor Vikram Jha (the UK)

Professor Joon-Shik Shin (South Korea)

Professor Michael Z. Wincor (USA)

Head of Undergraduate School of Medicine, Honorary Consultant Obstetrician, University of Liverpool & Liverpool Women’s Hospital NHS Foundation Trust. Since 2009 – member of Medical Education Advisory Group the Oxford Academy for Professional Health Education. External Examiner of King’s College London, Medical Schools in Peninsula and Sheffield. Successfully worked on implementation of a large number of national research grants in education, including INSPIRE grant from the Academy of Medical Sciences of Great Britain in 2012, 2013.

An active participant of UK National Student Survey aimed to improve training programs.

Head of the Centre of Oriental Medicine «Jaseng» and «Jaseng» Medical Foundation. Winner of 7 drug patents. He was twice given the Republic of Korea President Award for Civil Merits, and in 2007, Dale Carnegie Foundation noted his activity by certificate “Leader of Korea”. President of the Board of Korean Hospitals Medical Association (KOHMA), President of the board of Korean community for manual medicine Chung, President of the Board of Korean Oriental Medicine community for anti-aging, Vice-President of the Korean community for treatment of chronic diseases, a member of Korean Association Directors Council for the fight against drug abuse.

Assistant Dean for External Programmes of the University of Southern California.

Certified specialist in psychiatric pharmacy.

National expert of grant programs, consultant and lecturer in clinical pharmacology, insomnia problems.

Over the last five years, he was four times declared as “Professor of the Year” at the University.

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Issue 03 / November 2013 | isjm.kaznmu.kz

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