Progressive Crop Consultant - January/February 2022

January / February 2022 Review of Rhizoctonia Diseases of Row Crops CCA of the Year Keith Backman

VINEYARD REVIEW Use of Gypsum to Reclaim Salt Problems in Soils Diagnosing Vineyard Problems A New Biocontrol Approach for the Reduction of Pierce’s Disease in Vineyards

Volume 7: Issue 1

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January / February 2022

PUBLISHER: Jason Scott Email: jason@jcsmarketinginc.com EDITOR: Marni Katz ASSOCIATE EDITOR: Cecilia Parsons Email: article@jcsmarketinginc.com PRODUCTION: design@jcsmarketinginc.com Phone: 559.352.4456 Fax: 559.472.3113 Web: www.progressivecrop.com

IN THIS ISSUE

4

Review of Rhizoctonia Diseases of Row Crops

10

CCA of the Year Keith Backman Recognized at Crop Consultant Conference

14

Leaf Sap Analysis: A Forward-Looking Alternative to Tissue Sampling

18

4

Soil Microbes are Key Partners for Drought Management

VINEYARD REVIEW

22

Use of Gypsum to Reclaim Salt Problems in Soils

28

Diagnosing Vineyard Problems

32

A New Biocontrol Approach for the Reduction of Pierce’s Disease in Vineyards

40

Delayed Spring Growth and Grapevine Production During Drought

CONTRIBUTING WRITERS & INDUSTRY SUPPORT Luca Brillante Frank Martin Bronco Wine Co. Chair & Research Plant Pathologist, Assistant Professor of USDA-ARS Viticulture, Department of Viticulture & Enology, Khushwinder Singh California State University, Graduate Research Fresno Assistant, Department of Viticulture & Enology, Matthew Fidelibus Fresno State University Extension Viticulturist, UC Davis Stephen Vasquez Technical Viticulturist, SunSean Jacobs Maid Growers Technical Sales and Marketing Representative, Dr. Karl Wyant Agro-K Corp., Contributing Vice President of Ag Writer Science, Heliae® Agriculture, Chair Western Region CCA Anika Kinkhabwala Board of Directors Ph.D., Principal Scientist, A&P Inphatec George Zhuang UCCE Viticulture Farm Steven Koike Advisor, Fresno County Director, TriCal Diagnostics

UC COOPERATIVE EXTENSION ADVISORY BOARD

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Surendra Dara

UCCE Entomology and Biologicals Advisor, San Luis Obispo and Santa Barbara Counties

Kevin Day

Steven Koike Tri-Cal Diagnostics

Jhalendra Rijal

UCCE Integrated Pest Management Advisor, Stanislaus County

UCCE Pomology Farm Advisor, Tulare and Kings Counties Mohammad Yaghmour UCCE Area Orchard Systems Advisor, Kern County Elizabeth Fichtner UCCE Farm Advisor, Kings and Tulare Counties Katherine Jarvis-Shean UCCE Orchard Systems Advisor, Sacramento, Solano and Yolo Counties

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The articles, research, industry updates, company profiles, and advertisements in this publication are the professional opinions of writers and advertisers. Progressive Crop Consultant does not assume any responsibility for the opinions given in the publication.

January / February 2022

www.progressivecrop.com

3

Review of Rhizoctonia Diseases of Row Crops

By STEVEN KOIKE | Director, TriCal Diagnostics and FRANK MARTIN | Research Plant Pathologist, USDA-ARS

T

he soilborne fungus Rhizoctonia is an extremely important pathogen of plants worldwide. Hundreds of vegetable, field, fruit and nut, and ornamental crops are susceptible to this fungus. Rhizoctonia is common and severe on cereals and herbaceous row crops but can also cause disease on woody species. Found in soils throughout the world, this pathogen has evolved survival strategies that enable it to become established wherever it is introduced.

Despite the implementation of integrated pest management tools, Rhizoctonia remains a challenging pathogen that is difficult to control.

Understanding Rhizoctonia solani

Rhizoctonia is the genus name that refers to a group or “complex” of fungi.

Initially, fungal species were placed in this Rhizoctonia group because they shared certain features, such as the absence of asexual (anamorph) spores (remember that fungi can have two 1

2

"Rhizoctonia" refers to a diverse species complex

different phases or forms: asexual and sexual); a sexual (teleomorph) stage belonging to the Basidiomycetes; distinctive cell wall structures (septa) that divide the relatively thick hypha into sections, an existence that is primarily in the soil; and being mostly pathogenic to plants. For most row crops, the species Rhizoctonia solani is the most important pathogen.

Rhizoctonia is considered a “species complex” because of the many closely related species and subspecies that make up this group. Figure 1 outlines and breaks down this complicated, diverse group of organisms. The Rhizoctonia genus, first of all, can be divided into two major categories: (A) Those species that have two nuclei per mycelial cell (called binucleates) and (B) those species that have more than two nuclei per cell (multinucleates). R. solani and other species (e.g., R. oryzae and R. zeae) are in the multinucleate

Rhizoctonia solani most often attacks the belowground parts of plants, such as these cauliflower crowns, resulting in loss of root attachment and decline of the plants (all photos by S. Koike.)

Rhizoctonia solani forms tiny, matted mycelial clumps (sclerotia) that enable the pathogen to survive in soil for prolonged periods.

group. Note that each fungus listed in Figure 1 has two taxonomic names.

The Rhizoctonia name refers to the anamorph or asexual stage and is the phase of the fungus that infects plants

Continued on Page 6

3

4

Examples of Rhizoctonia species

Examples of anastomosis

There are two main

in each group, along with the

groups of Rhizoctonia

teleomorph (sexual stage) name

groups (AGs) in R. solani and disease tendencies

5 Isolates within AGs can be further differentiated based on physiology and genetic traits

A. Binucleate: mycelial

Rhizoctonia cerealis (Ceratobasidium cerealis)

cells have two nuclei AG1: seed infection, hypocotyl Rhizoctonia B. Multinucleate: mycelial

Rhizoctonia solani (Thanatephorus cucumeris)

rot, aerial web blight

Rhizoctonia oryzae (Waitea circinata)

AG2-1: root crop canker,

cells have more than 2 nuclei

crucifer wire stem Rhizoctonia zeae (Waitea circinata) AG2-2/IIIB and AG2-2/IV:

Growth rates

turfgrass brown patch, sugarbeet

Thiamine metabolism

root disease

Growth on carbon sources rDNA-ITS genetic sequences

AG3: affecting mostly potato AG4: infects many crops AG8: mostly on cereals, also infects potato roots There are currently 14 AGs for Rhizoctonia solani

Figure 1. Complexity of the plant pathogenic Rhizoctonia group, and placement of R. solani.

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January / February 2022

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January / February 2022 www.progressivecrop.com 5

Continued from Page 4 and causes disease. The second name is the teleomorph or sexual stage. This form is rarely found in the field and probably does not cause disease in plants; however, researchers employ the teleomorph names when studying the interrelationships between the various species. Our challenging fungal foe is therefore known mostly as Rhizoctonia solani (anamorph) but is also referred to as Thanatephorus cucumeris (teleomorph).

For lettuce and other head forming row crops, Rhizoctonia solani can cause a rot where the basal leaves are in contact with soil.

The R. solani species is itself very diverse and can be separated into distinct groups. Different isolates of R. solani fall into one of many anastomosis groups, or AGs (Figure 1, see page 4).

AGs are determined in the laboratory where two isolates are grown sideby-side in culture and the resulting reaction, whether the two hyphae fuse (compatible) or do not fuse (incompatible), is viewed under the microscope.

Isolates that are deemed compatible are placed together into a numbered AG. Incompatible isolates cannot be in the same AG. There are currently 14 AGs: AG1 through AG13, and AGB-1.

Seven of these AGs are further divided into subgroups. Molecular techniques are also available for AG classification; such techniques can be more accurate than searching for hyphal fusion under the microscope. Molecular methods, however, can be time-consuming to complete.

Categorizing R. solani isolates into these AGs is not an academic exercise but provides insights into how this pathogen functions in agriculture.

Different AGs possess different traits; while some AG isolates have a broad host range, others are more restricted regarding the crops they can infect. For example, R. solani AG2-1 tends to be the main pathogen that causes wirestem on crucifers, while AG2-2/IIIB and AG2-2/IV cause brown patch in turfgrass and root disease on sugarbeet.

R. solani AG3 is common on potato and causes stem and stolon lesions as well as black scurf on tubers. R. solani AG8 is primarily a pathogen of cereals but also infects potato roots. In con6

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Rhizoctonia disease category

Targeted plant tissue

Seed decay

Seed planted in the ground is invaded and rotted before the seed germinates.

Preemergent damping-off

Seed germinates; root and shoot are infected and killled before seedling shoot emerges above ground.

Post-emergent damping-off

Seed germinates and plant emerges above ground; root, crown, and lower stem are infected and plant collapses and dies.

Root rot of young plants

Young plants are healthy while germinating and emerging; after plant establishment, roots become infected and develop brown lesions.

Stem canker of young plants

Young plants are healthy while germinating and emerging; after plant establishment, crown and lower stem tissue in contact with soil becomes infected and develops sunken, brown cankers.

Foliar blight

Aboveground leaves, stems, shoots become diseased when the pathogen is splashed up onto foliage.

Bottom rot

For head-forming crops (lettuce, cabbage), bottom leaves in contact with soil develop brown lesions that later advance into extensive rots.

Fruit and pod rot

Fruits (e.g., cucurbit, tomato) and pods (e.g., bean, pea) in contact with soil develop lesions and rots.

Decay of fleshy tubers and roots

Fleshy underground vegetables such as sweet potato roots and potato tubers become infected and develop various symptoms.

Table 1. Categories of Rhizoctonia solani diseases of row crops

trast, AG4 has a broad host range and can infect many crops. So, identifying the AG status of an R. solani isolate can provide important information on the diseases caused by that isolate and the susceptibility of subsequent crops that might be placed in that field.

Finally, isolates belonging to the same AG are not all identical to each other.

While sharing the same AG designation, the isolates can differ physiologically in how carbon sources and other chemicals are metabolized, how

January / February 2022

fast they grow in culture, and other features. Isolates in the same AG can also differ genetically and have varying DNA sequences. This great diversity found within R. solani isolates accounts for the difficulty that researchers have in fully understanding this important plant pathogen complex.

Diverse Diseases Caused

by Rhizoctonia solani

Rhizoctonia solani causes different types of crop diseases, all of which are related to the soilborne nature of

Row crop hosts of Rhizoctonia solani

Rhizoctonia is one of the damping-off pathogens that can affect young seedlings, such as the Swiss chard plants pictured here: infected plants (left), healthy plants (right).

this pathogen (Table 1, see page 6). R. solani can be a seed pathogen. Once seed are placed in the ground, R. solani that is residing in the soil can invade the seed and kill it before it germinates.

Even if the seed germinates, R. solani can cause a decay of the roots and shoots before the seedling emerges above the soil surface; this early seedling disease stage is called preemergent damping-off. Post-emergent damping-off occurs if the diseased seedling is strong enough to grow above the soil surface, only to succumb and collapse shortly afterwards (Table 1). Collectively, seed decay, preemergent damping-off and post-emergent damping-off can result in loss of plants very early in the production cycle, causing stand loss in the field. Healthy seedlings that escape death at the seed and newly germinated stages remain vulnerable to this pathogen; established seedlings can still be infected by R. solani and develop root rots and/or lesions on stems in contact with soil (Table 1).

Rhizoctonia solani-contaminated soil particles can be moved up onto the leaves, causing a foliar blight on crops such as endive.

and plant base can take place, resulting in bottom rot. If fruits (e.g., cucumber) and pods (e.g., beans) happen to be in contact with infested soil, these harvestable commodities can become diseased (Table 1). Finally, sweet potato roots, potato tubers and other similar plant structures under the ground can suffer from lesions, rots, and defects from R. solani in the soil surrounding these fleshy plant parts. Table 2 lists

Continued on Page 8

Asparagus Bean Beet Broccoli Broccoli rabe Cabbage Carrot Celery Cauliflower Chinese cabbage Cilantro Cucurbits Endive Hemp Lettuce Pea Potato Radish Spinach Sweet potato Swiss chard Tomato

Disease

crown rot crown and root rot damping-off wirestem crown rot bottom rot root canker crater rot wirestem bottom rot root rot damping-off leaf blight root rot bottom rot foot rot Stem & stolon canker, tuber black scurf root rot root and petiole rot stem canker damping-off damping-off

Table 2. Row crop hosts of Rhizoctonia solani.

Plant leaves are not immune to R. solani. Field cultivation practices and splashing water can move bits of R. solani-infested soil up into the foliage of plants. Under favorable conditions, the introduced R. solani mycelium can colonize the leaf tissue and cause foliar blights in crops such as endive (Table 1). For head-forming vegetables such as lettuce and cabbage, the bottom leaves are unavoidably in direct contact with the soil. If R. solani is present in the underlying soil and if conditions (excess soil moisture) favor the pathogen, extensive rotting of the lower leaves

January / February 2022

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7

Continued from Page 7 some row crops that are susceptible to R. solani.

Disease Development

Rhizoctonia solani has evolved to be a challenging, persistent soilborne pathogen. Tiny, tightly clustered clumps of mycelium grow together to form resilient structures (sclerotia) that can withstand unfavorable conditions and allow it to survive for years without a plant host. These sclerotia are the main mechanism for survival, since R. solani is not a particularly aggressive or successful saprophyte in the soil environment. When a host plant grows next to sclerotia and favorable soil conditions are present, the dormant mycelium germinates and can infect the plant.

Diagnostic Considerations

Diagnosing Rhizoctonia diseases based only on symptoms is risky because Rhizoctonia is not the only soilborne pathogen that causes seedling damping-off, root rots and stem lesions of row crops. On spinach, damping-off and root rot can be caused by R. solani, Fusarium and Pythium; visually, one cannot reliably distinguish between the symptoms caused by these three pathogens. Cilantro crops can develop similar-looking root diseases caused

by R. solani and Fusarium. Cauliflower transplants are susceptible to both Rhizoctonia and Pythium, both of which cause the lower stem tissue to become discolored. Cauliflower disease diagnostics is further complicated because the root maggot insect feeds on lower stem tissue and causes symptoms identical to those created by R. solani. Precise and accurate diagnosis of Rhizoctonia diseases will therefore require lab-based examination and assays. Diagnosticians usually deploy culture tests in which surface sanitized bits of symptomatic tissues are placed in microbiological agar media. These scientists then use microscopes to examine the mycelium that grows out of the plated tissue. For most fungi, spores

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When testing plants, diagnostic labs use microscopes to look for the brown, relatively broad, distinctive branching mycelium that characterizes Rhizoctonia solani.

are important structures that diagnosticians rely on for fungal identification; since R. solani produces no spores, scientists must examine the hyphal structures or employ molecular assays to confirm this pathogen.

Managing R. solani

Rhizoctonia is a difficult pathogen to control. Attempts to manage this fungus will require the implementation of IPM practices.

Site Selection Plant in fields that do not have a history of Rhizoctonia problems and that have well-draining soils.

Crop Rotation Avoid planting a susceptible, sensitive crop in a field known to have significant problems with this pathogen. Rotate to crops that are either not susceptible or are less sensitive to damage caused by R. solani. Remember that some R. solani AG isolates have a very broad host range and can infect many row crops; however, other AG isolates show some level of host specificity and are pathogenic on only a few crops. It is therefore useful to know which AGs are present in the field.

Time of Planting In some cases, moving the planting date to a different time of year may help reduce losses from R. solani. Planting the crop in the warmer,

January / February 2022

drier part of the year allows the seedling to grow more rapidly and perhaps escape or minimize infection from R. solani.

Fungicides Plant seed treated with a fungicide.Note that the seed treatments used to protect against Pythium have little effect on Rhizoctonia. Fungicides applied to emergent plants have little benefit.

Resistant or Tolerant Cultivars There do not appear to be row crop cultivars that have genetic resistance to Rhizoctonia. However, if young seedlings escape infection early in development, the maturing stem tissue will later become resistant to infection by R. solani.

Sanitation Remember that as a soilborne pathogen, R. solani will be moved and spread via mud adhering to tractors and equipment. Prevent the introduction of R. solani into plant nursery and transplant facilities by using new or thoroughly sanitized containers and trays, disposing of used rooting media and other sanitation measures.

Comments about this article? We want to hear from you. Feel free to email us at article@jcsmarketinginc.com

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IMAGINATION

INNOVATION

SCIENCE IN ACTION

January / February 2022

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9

CCA of the Year Keith Backman Recognized at Crop Consultant Conference

Award celebrates agronomists’ five decades of commitment to industry and community.

By MARNI KATZ | Editor

T

Western Region CCA Past Chairman Jerome Pier, left, presented Backman with the CCA of the Year award at the Crop Consultant Conference in September.

his year’s Western Region CCA “He’s made a big impact on a lot of management plans, and without those Crop Consultant of the Year, Keith people in the Central Valley and on plans agriculture was going to get shut Backman, has been advising farmCentral Valley ag for sure,” Pier said. down by environmental activists,” Pier ers in the San Joaquin Valley since the “His understanding of nutrition in persaid. “This was the only way to show mid-1970s on fertilizer and irrigation manent crops is world class.” a good faith effort that we are doing management and the wisdom of matchsomething about it.” ing those nutrient and water applicaBackman is known for having an tions to the needs of the crops. Today, in advanced understanding about taking Those principals are based on the an environment of increased regulation and interpreting soil tests, and making relationship between nutrient and water and costs related to fertilizer inputs, that preplant and in-season recommendamanagement. prudence has grown more and more tions for fertility and irrigation manimportant. agement that are well suited to crop “Over the years I’ve come to realize that needs and soil status while also being so many of our deficiencies are irrigaBackman went to work with Nat Delenvironmentally sound. tion related,” Backman said. “Especially lavalle out of college in the 1970s and with nitrogen, you can’t have a good has been with Dellavalle Laboratory in Career of Service accurate nitrogen program unless you one way or another ever since. Today, As a member of the Western Plant have an accurate irrigation program.” he splits his time in semi-retirement Health Association’s Soil Improvement between traveling with his wife Gail Committee for more than 20 years, Farmers and PCAs today have a much and imparting his wisdom on plant Backman helped write the chapters on clearer understanding about the timing nutrition and water analysis to a new irrigation management and nitrogen of fertilizer applications and rates at generation of agronomists as part own- management for the revised editions of those particular times, he said. er of Dellavalle Labs. the Western Fertilizer Handbook. The latest edition was just released and has Where growers might have done a sinJerome Pier, outgoing Chairman of the Backman’s expertise throughout. gle or split application of 150 pounds Western Region CCA announced Backof N fertilizer a year, for instance, they man as the winner of the new CCA of He was also in on the ground floor of now make multiple applications based the Year Award at this year’s Crop Con- developing nitrogen management plans on yield estimate, soil conditions, crop sultant Conference in Visalia this past and was instrumental in drafting nitro- timing and soil and leaf analysis.

September. Pier said Backman sets the gen budgeting and checkbook methods standard for the region’s 1,300 CCAs that have been adopted for nitrogen “This is where the CCA earns their for his contributions to plant nutrition management plans by water quality money is helping a grower understand and soil science over his 40-year career coalitions. that,” Backman said. “Growers can’t and his contributions to the industry. guess anymore. They need proven solu“He basically authored these nitrogen Continued on Page 12 10

Progressive Crop Consultant

January / February 2022

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11

Continued from Page 10

nutrition imbalances by looking into an orchard.

tions and so more and more they are relying on the diagnosis of a CCA.”

Humble Ag Roots

Raised on a small farm south of Yuba City, Backman was seventh out of eight children in an agricultural family. While getting a Master’s degree in pomology at UC Davis, Backman focused his studies on boron toxicity in orchard crops. This is a problem that became more of an issue as deep-rooted permanent crops went in up and down the Valley and water shortages made leaching out of those root zones more difficult.

He worked early on in his college career under Kay Uriu, a professor in the UC Davis pomology department in the 1970s, who did ground-breaking research on tree nutrition status and was known for being able to spot

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As a result, Backman said even today he “can’t help driving by an orchard and looking at it and thinking ‘what can I do to get it growing more efficiently?’”

In addition to his service for the industry, Backman has been a scout master in his community for more than 20 years and until recently was an active member of his church choir.

It is Backman’s dedication to his industry, community and exceptional dedication to training new agronomists that led to his nomination as CCA of the Year, Pier said.

“I was honored by the selection,” Backman said. “I appreciate the recognition; it’s nice to know what you have done for the past 45 years is valued and to be

January / February 2022

able to see the changes in the industry from some of the things I’ve introduced.”

Among the changes he has seen in recent decades: more accurate nitrogen and water applications; more accurate monitoring to take appropriate actions at the appropriate times; and the application of science in making those applications using plant science and physiology to guide those decisions.

This is the second year Western Region CCA presented an annual Crop Consultant of the Year award to recognize outstanding individuals who have advanced crop consulting throughout their careers. For information on nominating a CCA visit the Western Region CCA website at https://wrcca. org/cca-of-the-year.

Comments about this article? We want to hear from you. Feel free to email us at article@jcsmarketinginc.com

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(707)621-8939 www.progressivecrop.com 13

Leaf Sap Analysis: A Forward-Looking Alternative to Tissue Sampling

By SEAN JACOBS | Technical Sales and Marketing Representative, Agro-K Corp., Contributing Writer

L

eaf sampling in agricultural crops is a long-practiced sampling method where the analysis of the collected tissues is used to assess crop nutrient status. Sampling whole leaves, drying, grinding, digesting and then analyzing the sample for nutrient levels has aided farmers in managing their crop nutrition programs and optimizing crop yield.

This method, however, has some inherent limitations. Primarily, the results of the analysis are providing nutrient levels for ALL nutrients in the sample, including those that are structurally bound within cell walls, the leaf surfaces (cuticles) and organelles. While the analysis is accurate in quantifying the nutrient levels in the sample, these bound nutrients are largely immobile and unavailable to developing leaves and fruit.

Both over-applications and mistimed applications of nutrients can negatively affect crop yields and quality.

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Progressive Crop Consultant

January / February 2022

Additionally, any nutrients found on the outside of the leaf or embedded in the leaf cuticle are included in the results. As an example, if a calcium carbonate material were applied foliarly to a crop and then tissue samples were pulled, the analysis would demonstrate that our tissue’s calcium levels increased. On the other hand, calcium carbonate materials have very poor foliar uptake and are commonly used as solar protectants or sunscreens. As a sunscreen, it is necessary that the material remains on the leaf surface to do its job, but a standard tissue sample analysis cannot differentiate between “in” or “on” the leaf. Further, tissues being prepped for analysis may be rinsed or washed in an attempt to alleviate leaf surface contaminants. What is commonly overlooked with this practice is the effect that rinsing can have on nutrients within the leaf. For instance, potassium, calcium, magnesium, manganese, nitrogen, phosphorus and zinc can all be leached to varying degrees from the leaf tissue with water.

Now, if the grower is analyzing the leaf tissue because it is the crop, then knowing the nutrient levels of the entire leaf structure and surface is appropriate.

However, many growers are not selling leaves but are using the leaves as the machinery to develop the structure of the plant and produce the end crop.

Whether that end crop is a tuber, fruit, nut, seed or simply a flower, knowing the number and quantity of nutrients available for assimilation within the plant as well as the balance among these nutrients may spell the difference between a mediocre crop and a stellar crop. Knowing and adjusting the nutrient balance is crucial to nutrient performance and preventing fruit nutrient disorders which can impact crop storability and shelf life. Other characteristics such as fruit sugar levels, size and color also can be positively impacted by proper nutrition.

Forward Looking Analysis

An alternative method to leaf tissue analysis is sap analysis. With sap analysis, leaves are sampled in sets with new leaves and old leaves collected separately without petioles. At the lab, a proprietary process under the NovaCrop brand then extracts the sap from the leaf. This process is done without rinsing, drying, grinding, cutting or crushing the leaves and the extracted sap is largely free from leaf structural components and surface contaminants.

This results in the extracted sap being more similar to a blood sample than the biopsy approach akin to classical tissue sampling and analysis. In addition to analyzing the samples for 19 nutrients and five other nutrient and metabolic indicators, having the sap of both new and old leaves analyzed separately allows for the comparison of nutrient uptake, mobility and remobilization within the plant. This comparison is also valuable for assessing the movement of sugar in the plant, which is the plant’s initial building block and energy source.

Continued on Page 16

By quantifying the metabolically active and available nutrients in the sap and assessing their balance, growers are able to determine not just if nutrient deficiencies exist but also the future potential for nutrient deficiencies (photo

by Cecilia Parsons.)

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15

Continued from Page 15 Minerals, sugars and nitrogen-containing compounds, such as amino acids and proteins, found in the sap represent the majority of plant nutrients that are immediately available for use. By intentionally sampling and analyzing leaf sap, the results provide a forward-looking picture of the nutritional environment in which the plant is currently growing. With this information, deficiencies, toxicities and nutritional imbalances can be identified and corrected in a proactive manner, often before their physiological effects are visible. With traditional tissue analysis, the results are providing information largely about what has already happened nutritionally, leading to decisions being reactive in nature.

As the demand for agricultural products continues to increase, farmers are often turning to more aggressive fertility programs, which frequently leads to over-applying nutrients or missing the best opportunity for the application.

Both over-applications and mistimed applications of nutrients can negatively affect crop yields and quality. The balance of nutrients within the plant can be upset by an over-applied nutrient. This can happen with foliar-applied nutrients as well as soil-applied nutrients. In some instances, as with nitrogen, it can alter the expression/ regulation of genes and lead to a shift in growth toward vegetative and away from fruit development. In other cases, the over-applied nutrient can create nutrient imbalances that present as deficiency symptoms of other nutrients despite adequate concentration levels in the sap. This can be described as likekind interactions where minerals of similar charge are competing with each other for space within the sap (cations affect cations and anions affect anions.)

Physiological responses within the

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plant to an applied nutrient can modify the uptake or physiological activity associated with other nutrients, either positively or negatively.

Nutrient Availability

By removing unavailable nutrients from the analytical picture, the concentrations of available nutrients and their interactions are more easily seen in the analysis and accommodated for in the grower’s nutrition program. In the soil, colloidal and mineral properties influence how various nutrients, specifically cations, populate the cation exchange locations. A key takeaway is that these soil interactions occur primarily with available nutrients.

The same is true within the plant with nutrient interactions primarily between available nutrients not unavailable and structurally bound nutrients.

This is where sap analysis shines.

By quantifying the metabolically active and available nutrients in the sap and assessing their balance, growers are able to determine not just if nutrient deficiencies exist but also the future potential for nutrient deficiencies. With a NovaCrop sap analysis in hand, growers are able to evaluate the balance of nutrients in their crop and better understand how excessive levels of nutrients may impact the uptake and/or activity of others. Through this analytical report, a grower might determine that the most efficient way to increase the level of a certain nutrient is NOT by applying more of that nutrient, but rather is best achieved by decreasing the rate of other applied nutrients and restoring balance.

Over-application of nutrients affect the safety of ground and surface water for human consumption and the wildlife dependent on those water sources. In an ever-increasingly regulated world,

January / February 2022

leaching and runoff of nutrients caused by over-application are not merely wasted money and crop potential, but could result in the grower being fined thousands of dollars, reclamation fees and civil judgements. Fertility management plan modifications, when based on NovaCrop sap analysis, and coupled with soil analysis, improves fertilizer use efficiencies and decreases over application of nutrients.

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Maximizing Nut Set & Size Under Dry Conditions

The Record-breaking drought and heat seen across California in 2021 is forecasted to continue into 2022. With drier springs comes reduced disease pressure to almond blooms and nutlets. This often means that fungicide applications at pink bud and bloom can be decreased or eliminated. While it can be tempting to leave the sprayer in the barn, almond growers’ nut set, size and yield depend on earlyseason foliar nutrition.

Growers that want to achieve maximum economic yield, however, would be wise to reallocate their fungicide dollars to where they can get the best return. The value of a good nutritional program cannot be overstated. In fact, well designed nutrient programs are even more essential in a dry year. Without moisture from rain, pollen and flowers desiccate rapidly. Desiccation reduces pollen’s viability shortening the bloom receptivity window which reduces nut set and yield. Starving the developing flowers and nutlets of essential nutrients intensifies the reduction.

The right nutrients applied during the pink bud and bloom window can make all the difference. Vigor-Cal-Bor-Moly, a sugar complexed calcium foliar combined with boron and molybdenum, is an excellent fit for pink bud and bloom time sprays to improve nut set and quality.

With a shorter bloom window, supplemental boron ensures successful germination and pollen tube development—also known as nut set.

Molybdenum, a key component of nitrogen metabolizing enzymes and others, facilitates stress responses, vascular development, and growth. Symspray, Agro-K’s seaweed product, when applied during pink bud and bloom can reduce the effects of environmental stress on the flowers, extending bloom and increasing pollen receptivity even when it is dry.

By adding AgroBest 9-24-3 to the tank with Vigor-Cal-Bor-Moly during cell division, calcium and phosphate work together to promote larger and heavier nuts. AgroBest 9-24-3 is a high phosphate/low potassium blend that delivers the phosphate energy the tree needs to maximize nut cell division, nut size and nut retention. AgroBest 9-24-3 is the most cost-effective liquid phosphate available. It delivers more phosphate per dollar at peak demand timing and is specifically designed with minimal potassium content for early season foliar applications that won’t waste dollars or antagonize calcium during nut and leaf cell division.

Ultimately, almond growers that leave their sprayers in the barn will produce smaller, lighter nuts and lower yields. Reducing the number of dry-season fungicide sprays leaves more money in the budget for a science-driven foliar nutrition program. Reallocating some of these funds for applications that drive higher yields and increase nut size is a smart way to ensure the biggest benefits from less-than-ideal environmental conditions. After all, growers still need to maximize their economic yield, as their costs and expenses continue to go up, not down.

While foliar nutrition is essential during the pink bud and bloom window to maximize economic yield, it is very important throughout the season. A dry year requires almond growers to think critically about the key nutrients they apply at each growth stage to produce more nuts with less water. Implementing a Science-Driven™ nutrient approach this year will deliver more pounds of nuts per unit of water resulting in higher economic returns per acre for you.

Products Available At: ®

Science-Driven Nutrition ™ Corporation 8030 Main Street, NE • Minneapolis, MN 55432 • 800-328-2418 • www.agro-k.com

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17

Soil Microbes are Key Partners for Drought Management

By DR. KARL WYANT | Vice President of Ag Science, Heliae® Agriculture; Chair, Western Region CCA Board of Directors

M

anagement practices that improve soil health and soil quality have gained considerable attention over the past few years, and especially during the past year, as drought conditions have impacted large areas of North America. In this article, I focus on how the living, biological components of the soil (e.g., bacteria and fungi) can be key microbial partners in your future drought management strategy.

Soil Health and Drought Management

Figure 1. Here are two concepts to help organize the contribution of microbes to soil health and structure (Concept 1) and the substances that are released by the microbes themselves (Concept 2) that help crops get through a drought period (courtesy K. Wyant.)

I detail how soil microbes impact soil physical properties, including the structure (e.g., aggregation and pore space) and the ability of the soil to move and store water. Additionally, I explain how soil microbes can help crops get through drought conditions using the substances they secrete.

Finally, I close with a call to action to measure your soil biology so you can make management decisions now before the next season gets underway.

If needed, a CCA can help you interpret data and make an actionable plan that can help tackle the continued drought conditions that are expected for the near future.

Let us begin with a quick reminder of what soil health means to a grower and how it is connected to the living component underground and drought management. Soil health is directly related to the interaction, or lack thereof, between organisms and their environment in a soil ecosystem 18

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and the properties provided by such interactions. When you think of soil health, think of the biological integrity of your field (e.g., microbial population and diversity) and how the soil biology supports plant growth. There is a direct link between soil health and how a soil can be managed to meet the challenges of drought conditions.

as water infiltration. If you are not capturing and moving water into the soil, you will have a tough time storing water in your field. Simply put, healthy soils have good structure, which excel at receiving and storing moisture. But how exactly do microbes improve water penetration and infiltration?

Concept 1: Soil Microbes Help Increase Water Penetration and Infiltration

Abundant and diverse soil microbial communities produce “free” services for your farm soil, including the ability to receive and store moisture. The key to this ability lies in the ability of microbes to contribute directly to improving soil structure by binding soil particles together, which, in turn, helps water move from the soil surface and into the root zone.

Soil microbes help restore soil structure which helps water move from the soil surface downwards. This is known as water penetration. Once the water has penetrated the soil, it moves down into the soil for storage. This is known

Soil bacteria produce a sticky, gluelike gel called extracellular polymeric substances (EPS) that form a protective slime layer around bacteria as they grow. The EPS acts as an adhesive to bind soil particles, thereby improving

Soil Microbes and Drought Management

Soil microbes impact your ability to manage drought via two major pathways, i.e., the “Two Concepts of Drought Management” (Figure 1).

January / February 2022

A Living Soil Helps with Drought Management

Figure 2. The soil on the left has poor soil health and soil structure while the soil on the right has excellent soil health and, as a result, the two fields have substantial differences in soil quality and their ability to mitigate drought stress (courtesy K. Wyant.)

overall soil structure. Fungi, another important group of soil microbes, produce miles of microscopic threads in the soil called hyphae. The threads capture and “tie” soil particles together, like a net, which improves overall soil structure.

Fungi also produce a sticky protein-like substance called glomalin which, like EPS, helps bind your soil structure together via adhesion of particles.

Key Message: Soils with healthy microbial populations can restructure and re-aggregate the soil, which leads to better soil structure overall. Good soil structure allows for water (e.g., snowmelt, rainfall and irrigation water) to move from the soil surface (penetration) to below the soil surface for storage around and on the soil particles themselves (infiltration). This results in a number of benefits, including reduced runoff losses.

Research and grower experience corroborate this connection as reports show that soils with good structure often can store more water relative to degraded control fields nearby. Good soil structure also reduces runoff losses as water quickly moves downwards instead of horizontally across the soil surface, which carries materials off the field. Thus, soil microbes can be crucial partners for capturing and storing soil moisture, which will certainly come in handy during forecasted drought periods.

versity when compared to the “business-as-usual” farm soil on the left side. Now that you have refreshed your snapshot

Continued on Page 20

Advertorial Oregon State has developed an Aglime Score which is basically an efficiency rating. Particle size or mesh size is key to this rating and is the primary indicator of reactivity. Studies have shown that pulverized limestone smaller than 40 mesh (size of table salt) are considered 100% effective and are the quickest to dissolve in the soil to release calcium and adjust soil pH. Mildly acidic water and soil conditions will dissolve finely ground limestone. For example, the pH of rainwater in California is typically around 5.7, which is enough to dissolve our aglime that is broadcast. Our pulverized limestone products average 85% passing 100 mesh (diameter of a human hair). Remember aglime quality increases when particle size decreases.

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Concept 2: Robust Microbial Communities Release Substances to the Soil Which Can Help Crops Get Through Periods of Drought This next concept is not so easy to visualize like changes in soil structure and the ability to store water. Imagine the microbial community on the right side of Figure 2 for the next few sentences and contrast the microbial abundance and di-

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19

Continued from Page 19 of the microbial community, we can turn our attention to the benefits that improved microbial abundance and diversity bring to a drought affected soil. Recent work has shown that soil microbes help crops get through periods of drought stress via the substances they release into the soil around the roots. These molecules include osmoprotectants and antioxidants, to name just a few (see first reference for a deeper dive).

Key Message: A hidden benefit of maintaining a thriving community of microbes during a drought are the substances they secrete. For example, osmoprotectants play a key role in managing challenges with plant water balance under drought conditions. In another example, antioxidants help mitigate oxidative stress and internal plant cell damage observed under drought stress. Studies show that when a robust microbial community releases certain substances belowground, a crop is better able to weather the stress of drought (e.g., low rainfall, higher temperatures) more successfully aboveground.

Managing Soil Biology

Now that we have examined how soil microbes can be crucial partners under drought conditions, we can now turn our attention to a crucial next step: managing the soil biology. I will walk through the basics on how to measure the biological activity of the soil and make the case to ask your trusted CCA for assistance if this management strategy is new to your operation or if you need help with interpretation of the results.

Tests are commonly used to measure chemical constituents of the soil (e.g., pH, nitrate, phosphate, etc.) or physical aspects of the soil (e.g., soil texture, cation exchange capacity). However, these tests do not determine how “alive” the soil is. You can accomplish this goal with a broad set of soil tests that target the living components of soil. Soil biology tests are diverse and include measurements of carbon 20

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Test Name

Speed of Response

What It Measures

CO2 Burst Test

Measures the activity of the oxygen-using soil microbes (fungi + bacteria) as they “breathe out” CO2 during their daily activities. Higher CO2 measurements indicate more microbial activity at the time of sampling.

Changes daily

ACE Protein Test

Measures how much “protein-like” substances are in a field. Higher ACE protein values indicate more organically bound nitrogen that microbes can release for future plant uptake.

Changes weekly or monthly

Active Carbon Test

Measures the amount of materials available to “feed” soil microbes. Higher active carbon test values indicate that there is more food available to fuel microbial work.

Changes weekly or monthly

Water Holding Measures how much moisture your soil can Capacity Test hold, which is influenced by soil biology and Aggregation Test

structure. Higher values indicate better water storage on a field.

Changes slowly over growing season

Measures the physical structure of your soil, which is strongly influenced by soil biology. Larger particle sizes typically indicate better soil structure.

Changes slowly over growing season

Table 1. There are several soil tests available to help you quantify the living components of your soil and their response to field management decisions. Please see the “Comprehensive Assessment of Soil Health” from Cornell University for an exhaustive list on what is available or call your local lab. Listed are a few common testing options in ag laboratories.

dioxide respiration, extraction of DNA for microbial community analysis, and other key metrics (Table 1), depending on what parameter you are interested in measuring and your patience level to see a measurable change.

Vice President of Ag Science at Heliae ® Agriculture. To learn more about the future of soil health, you can follow his webinar and blog series at www.phycoterra.com.

Suggested Reading Testing the biological components of the soil is new to many growers and Harnessing rhizosphere microbiomes some have reported frustration about for drought-resilient crop production which test to choose, how to design a - https://www.science.org/doi/10.1126/ sampling program, and how to inscience.aaz5192 terpret and write an actionable plan based on the test results. This is where a The Connection Between Your Soil Certified Crop Advisor can step in and Structure and Soil Moisture - https:// help reduce the learning curve. phycoterra.com/connection-betweensoil-structure-soil-moisture-crop/

Final Thoughts

Soil microbes can help you mange drought in ways that are readily observable (e.g., changes in soil structure and water holding capacity) and in ways that are not (e.g., release of substances that help with drought stress).

In any event, microbes are essential partners for dealing with drought conditions and their usefulness should be leveraged in any crop production program.

Dr. Karl Wyant currently serves as the January / February 2022

Biological Management Practices to Maximize Soil Quality - https:// progressivecrop.com/2021/05/managing-soil-structure-and-quality/ Comprehensive Assessment of Soil Health (The Cornell Framework) https://soilhealth.cals.cornell.edu/training-manual/ Comments about this article? We want to hear from you. Feel free to email us at article@jcsmarketinginc.com

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21

Use of Gypsum to Reclaim Salt Problems in Soils Three-year viticulture case study illustrates response of gypsum application to soil, grapevines and fruit.

By LUCA BRILLANTE |Bronco Wine Co. Chair & Assistant Professor of Viticulture, Department of Viticulture & Enology, California State University, Fresno and KHUSHWINDER SINGH | Graduate Research Assistant, Department of Viticulture & Enology, Fresno State University

Figure 1. Effect of severe salt stress on young and mature grapevines (all photos courtesy L. Brillante.)

S

oil salinization is caused by excessive accumulation of salts in the soil and is one of the most severe land degradation problems. Globally, salt-affected soils are estimated to be about 2 billion acres and are expected

to increase in the future. In California, about 4.5 million acres of irrigated cropland (more than half) are affected by salinity, causing significant problems to the state’s agriculture. When salinity levels exceed critical thresholds in the soil, the plants cannot reach their full genetic growth potential, development and reproduction.

Causes and Consequences of Soil Salinity

Soil salinity can be related to the origin of the soil (e.g., in soils from land that was once submerged under the level of the sea or a lake.) It can also be caused by humans. Irrigation in dry areas exacerbates the problem as water contains 22

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January / February 2022

salts that are left in place after evapotranspiration, and there is not enough rainfall to help with the leaching (i.e., the process of washing off excess salts from the surface toward the deeper layer of the soils and out of the reach of plants.)

Salinity can be caused by excess in different kinds of salts, including table salt (NaCl), potassium chloride (KCl), etc.

Table salt is the most common and most problematic. It is composed of sodium ions (Na+), which negatively impact soil physical-chemical properties and create an osmotic stress in plants, and chloride anions (Cl-), which are toxic to plants.

Accumulation of sodium in soils causes swelling of clays, destroys soil structure and reduces infiltration and water holding capacity (Figure 2). This reduces the oxygen & water availability to roots. Excess of salts in the soil generates osmotic stress, which affects grapevine physiology and performance. At low rates, it is a chronic problem that disturbs grapevine water relations, causes stomatal closure in grapevine and reduces leaf and crop size. At high rates, it can create toxicity problems that can lead to premature leaf senescence and plant death (Figure 1, see page 22).

Salt buildup can result in three types of soils: saline, saline-sodic and sodic. Salinity & sodicity terms are used interchangeably very often. However, salinity refers to the concentration of salts (of all kinds) in the soil. Sodicity is associated with the proportion of sodium in pore water or adsorbed to the mineral surface. In saline soils, there are enough soluble salts of all kinds to injure plants. Sodic soils are low in soluble salts but relatively high in exchangeable sodium. Saline-sodic soils have a high content of salts and high content of sodium relative to calcium and magnesium salts.

The electrical conductivity of soil extracts can measure soil salinity, as with more salts in the water, it is easier for the

Continued on Page 24

Figure 2. Effect of salt stress on the soil, showing reduced infiltration, surface crust formation, and clays’ dispersion.

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23

Continued from Page 23 current to flow. Sodicity of soil is indicated by the exchangeable sodium percentage (ESP), which is the soil cation exchange capacity occupied by sodium, or by the measure of sodium adsorption ratio (SAR), which represents the amount of sodium with respect to calcium and magnesium. Saline, sodic and saline-sodic soils can be differentiated according to their physical-chemical properties. Soils with EC > 4 dS/m, SAR <13, pH <8.5 are classified as saline soils; soils with EC <4 dS/m, SAR >13, pH >8.5 are classified as sodic soils; and soils with EC >4 dS/m, SAR >13, pH <8.5 are classified as saline-sodic soils.

Alleviating Soil Salinity

Alleviation of salt-related problems is of crucial importance to reduce the impact on crop performance and ensure the profitability of agriculture. This can be done by decreasing the amount of Na+ ions, destroying soil structure and replacing them with Ca+ ions. Adding calcium helps maintain a favorable electrolyte concentration in the soil solution, thereby preserving the physical and chemical properties such as structural stability and clay flocculation, which encourages better root penetration and air and water movement through the soil. Ca2+ ions have a much higher flocculating power than sodium and potassium ions due to their charge and size.

Thus, Ca2+ ions have a higher affinity for clays and can easily

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January / February 2022

replace Na+ ions during soil reclamation practices to improve soil properties.

For this purpose of alleviating salt buildups, soils are treated with calcium-based amendments, the most common being the application of gypsum (CaSO4·2H2O). Other products, such as calcium chloride (CaCl2), calcium carbonate (CaCO3) and sulphuric acid (H2SO4), can also be used for reclamation of saline soils but are not as effective as gypsum, and they are destined to specific conditions.

Calcium chloride contains chloride anions that are known to be toxic to plants and, in particular, grapevines. Sulphuric acid does not directly contain calcium required to replace the Na+ ions in the soil. Hence, it is only effective in calcareous soils (rare in the U.S.), where it can react with the calcium carbonate to free the calcium already in place. Calcium carbonate (CaCO3), or lime, is alkaline and significantly less soluble than gypsum. It is generally used to reclaim acid soils.

It cannot provide sufficient Ca2+ ions for effective Ca2+- Na+ exchange as it can free Ca2+ ions only in acid soils, while salt-affected soils are generally alkaline.

Gypsum as a Solution

Gypsum is a soft sulfate mineral composed of calcium sulfate dihydrate with the chemical formula CaSO4∙2H2O containing two molecules of water inside every single crystal. Calcium sulfate can also be found in two more phases: calcium sulfate anhydrite (CaSO4), which does not contain water of crystallization, and calcium sulfate hemihydrate, also called bassanite (CaSO4∙1/2H2O), which only contains a half-molecule of water per crystal. Severe pressure, temperature and other natural conditions make gypsum outcrops lose water molecules and form anhydrite and bassanite.

Besides a high concentration of calcium and sulfur, pure-quality gypsum contains 21% water, giving it peculiar properties (e.g., different solubility) with respect to the other forms of calcium sulfate. Of particular importance under dry conditions is the ability of plants to access and use the water in the crystalline structure of gypsum.

The use of gypsum crystallization water by organisms is a critical water source for life under dry conditions, as recently demonstrated by Palacio et al. 2014. These authors reported that in natural conditions and during summer, the sap of shallow-rooted plants is 70% to 90% derived from gypsum crystallization water. The extraction and use of water molecules from gypsum by plants is accelerated in hot temperatures. Microporosity of gypsum also offers protection to the soil microbes and promotes relatively abundant and diverse microbial life in dry conditions. Soil microbes may in turn increase the availability of inorganic compounds to plants.

Gypsum is best applied routinely, as frequent irrigations leach out calcium from the root zone. This is even more import-

Continued on Page 26

Better Nutrition Grows Firmer Fruit Fruit growers are producing firmer, higher quality table grapes, wine grapes and strawberries with Pacific Gro. Firmer fruit has longer shelf life, earns a premium price, and it tastes better.

We’re seeing this in both organic and conventional production — table grapes in Delano, strawberries in Santa Maria, apples and cherries in central Washington, berries in Oregon—and vegetable crops too.

Calcium plays a key role in moving other nutrients into the fruit. It provides structural support to cell walls of plants and root development.

There are many sources of calcium, and big growers have tried them all. However, growers see an additional benefit with Pacific Gro, providing plants readily available calcium and many other essential nutrients that help crops thrive.

Table grapes grown near Delano, California

Pacific Gro should be viewed as a core input and key contributor to any crop program. It helps microbes get established, especially the all-important fungal components.

Fish oil and chitin provide the necessary building blocks for microbes to multiply, mineralize nutrients, and create healthier soils. Amino acids immediately convert into plant available nitrogen and promote calcium absorption. Natural fulvic acids help chelate nutrients and strengthen crop drought and heat tolerance. Pacific Gro’s complex biological structure is delivering exceptional crop results to growers.

Please note that Pacific Gro can be applied through drip lines as well as pivot sprinklers on row crops.

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25

Figure 3. Gypsum application in our study.

Continued from Page 24 ant when water for irrigation is alkaline. If gypsum is not applied regularly and calcium content decreases in the soil, the soil tends again to get compacted and the infiltration of water slows down, creating stress to plants.

Figure 4. Infiltration rate measured by the single ring infiltrometer method in the year after the application. See the text for treatments corresponding to code.

It is recommended to spread gypsum on soils as an application through low-volume irrigation sources (i.e., drip and sprinklers) and is shown to work best with irrigation water of low salinity, i.e., about 0.1 ds/cm.

Liquid gypsum application can increase the water infiltration to greater depths under the emitters over time due to soil particle binding and aggregation properties of calcium. When gypsum is applied through drip lines with irrigation waters of high bicarbonate content, it requires cautionary measures and lowering of pH (<6.5) because of the formation of calcium bicarbonate precipitates.

Gypsum Research

With the intent of clarifying the effectiveness of gypsum to reclaim salt-affected soils and to provide application guidelines in vineyards, we performed a three-year-long project where we monitored the response of soil physics, grapevine physiology and fruit composition to different dosages and forms of CaSO4 (anhydrite, CaSO4 & gypsum CaSO4∙2H2O) in synergy with organic matter.

Figure 5. Amount of calcium in the soil solution in the year after the treatment application. The soil samples were extracted with the ammonium acetate method and measured with MP-AES. See the text for treatments corresponding to code.

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January / February 2022

The experiment was performed from 2019 to 2021 in a Merlot vineyard located in a fine loamy sodic soil on the southwest side of Bakersfield, Calif. After the first season of measurements (2019), to ensure no differences across treatments before application, we broadcasted chemical amendments in winter 2020 in bands under the vines (Figure 3, see page 26). The experiment was a completely

’

Of particular importance under dry conditions is the ability of plants to access and use the water in the crystalline structure of gypsum.

Figure 6. Yield per vine (1kg = 2.2 pounds) in the year of treatment application (2020) and in the year after application (2021).

randomized block design with six treatments replicated four times, and each replicate was 0.2 acres large.

The treatments were applications of gypsum at different rates: 2.5 t/ac (50% reclamation rate, code 50G), 5.1 t/ac (100% reclamation rate, code 100G), 10.2 t/ac (200% reclamation rate code 200G), application of anhydrite at 5.1 t/ac (100% reclamation rate, code 100A) and addition of compost to gypsum (5.1 t/ac gypsum + 1t/ac compost (code 100GC)). We included one untreated control (code CTRL). All treatments were applied in one single application at the beginning of the experiment.

Calcium amounts in the soil extracts (obtained after treatment of soil samples with ammonium acetate) increased in all treatments with respect to the control one year after the application, both in the top eight inches and at a depth between 8 and 16 inches. We observed the largest increase in the 100G, which had 28% more exchangeable calcium ions on the soil particles than the control in the top eight inches and 15% more in the 8- to 16-inch depth (Figure 4, see page 26).

Sixteen months after the application, we started observing effects on water infiltration rate in the soil, with the untreated control showing the lowest water infiltration rate. The 200G and 100GC showed the highest infiltration rate with 65% and 63% improvement over the control, respectively (Figure 5, see page 26).

as the following year (5%). The other treatments did not have positive effects on yield. We did not observe significant differences in sugar content (Brix), pH or titratable acidity of musts across treatments.

This trial showed that gypsum effectively reduces the adverse effects of salt stress on soils and vines by increasing calcium ions at the surface of clays. The increase of calcium ions improves soil structure with positive returns on infiltration rates and the increase in soil health promotes grapevine performance and increases yield. The addition of compost enhances the positive effects of gypsum.

References Palacio et al., 2014 The crystallization water of gypsum rocks is a relevant water source for plants. Nature Communications 5, 4660.

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We did not observe notable differences in plant water status measured by a pressure chamber (water potentials), neither in photosynthesis nor transpiration across the treatments. In the two years after the treatment, we observed higher yields in the 200G and 100GC with respect to all other treatments and the control (Figure 6). The response was more robust in the year of the application, in particular for the 100GC, with plants producing 15% higher yield than the control, but only 2% more than the control in the second year. At the same time, the effect of the 200G was more consistent and was higher (8%) with respect to the control the same year as well January / February 2022

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27

Diagnosing Vineyard Problems

Data Collection and Understanding Patterns to Mitigate Damage and Yield Loss

By STEPHEN VASQUEZ | Technical Viticulturist, Sun-Maid Growers

T

he 2021 season was a challenging year for western grape growers.

After an unusually dry, cool winter, many growers observed poor shoot growth and various fruit maladies from bud-break through harvest. Delayed spring growth, vine mealybug and leaf hopper infestations, heat waves and water shortages were just a few of the issues that caused problems for grape growers.

Farmers and viticulturists were busy trying to diagnose various vineyard problems from shortly after bud-break through leaf fall. Diagnosing vineyard problems as soon as possible is important for minimizing yield and quality losses, especially when caused by pests that can be managed before moving into neighboring vineyards. However, when a quick diagnosis cannot be made, it is important to survey the vineyard and collect as much information as possible so damage can be mitigated.

Prompt Data Collection

When trying to diagnose abnormal plant growth, it is important to collect as much data as possible near the time that it was first observed. Data in the form of dates, symptoms (e.g., abnormal growth), signs (e.g., insects), records, etc. are important for you and those you may consult with in determining the cause of poor growth. Table 1 highlights important information to collect for proper diagnosis. The most basic information focused on vineyard characteristics, such as variety/rootstock combination, weather, soil, irrigation type, grape tissue analysis, etc., are typical data needed to solve vineyard problems. Sometimes, it can take days or weeks of consideration to determine what has affected the vines. However, there are times when a problem remains unsolved, even after data have been collected and reviewed by multiple experts.

After basic vineyard characteristics, re-

Table 1. Data collected to help iden2fy vineyard problems VINEYARD CHARACTERISTICS

- Variety - Rootstock - Vineyard age - Soil type - Irrigation type - Surrounding vegetation (crops and native)

REPORTS - Yield and quality - Pesticide use - Fertilizer use - Soil maps - Soil and water amendments - Establishment - Production practices - Previous crops grown - Source of plant material

ports (see Table 1) of varying types are important pieces of information for deciphering vineyard issues. Phenological dates, yield and quality, fertilizer and pesticides used, etc. give clues about what has happened this season and in past years that may correlate with a particular issue. The more reports that are available for review, the easier it will make solving the problem.

Photos are a great tool because they can easily and quickly be shared via email or text. Today’s phones are equipped with a camera that can capture both photos and video that make documenting vineyard problems easier. However, there is a difference between a photo and a great photo that can help solve the cause of symptoms. Great photos show detail that can aid in determining the cause of symptoms. For example, a picture of a single leaf on the bed of a truck probably won’t help identify a problem. A more useful photo would

PHOTOS - Leaves - Canopy - Fruit - Roots - Cross section of canes, cordons, trunk - Pests - Surroundings - Aerial images

Table 1. Data collected to help identify vineyard problems

28 Table Progressive Crop Consultant / February 2022 pa>erns found in vineyards.

2. Some likely causes and January symptoms* of certain

LABORATORY ANALYSIS - Water - Soil - Tissue - Leaves - Stems - Roots - Insects - Diseases

highlight multiple symptomatic leaves showing their location on the grapevine and in the vineyard, which may highlight the root cause of a problem.

Driving and walking vine rows with a soil map in-hand can be essential for assessing problem areas. Surveying the vine-

Continued on Page 30

Water, soil and tissue laboratory analysis when taken annually help identify trends over seasons. The best approach is to pull samples at specific times (i.e., growth stages) each year so the information is available when decisions need to be made.

For example, taking water samples at the beginning of the growing season will help determine how much nitrogen is coming from irrigation water sources. Once known, planned fertilization rates can be adjusted depending on the amounts in the water. Excess nitrogen applied during the season without knowing what is in well water can contribute to poor fruit quality and may further contaminate the aquifer.

Biotic vs Abiotic

Vineyard issues affecting vine health and fruit production fall into two categories: biotic or abiotic. Issues caused by insects, fungi, bacteria, viruses, rodents, birds and other living organisms are biotic. Abiotic includes non-living factors like climate, soil compaction, water, pH, nutrient presence or lack thereof, etc. Biotic factors tend to be the most common reason for poor grapevine health but aren’t always the primary cause.

Trying to separate symptoms between biotic and abiotic vine health issues can take time to sort through since symptoms can have multiple causes. For example, a vineyard may be showing signs of decline due to root rot infections. However, the real cause may be the presence of hardpan not allowing water to drain. Water accumulating around vine trunks often leads to fungal infection, decline and vine death. Symptoms from both fungal infections and poor water penetration can have similar symptoms (weak vine growth with chlorotic foliage). Identifying the primary cause is important so a solution can be developed and implemented.

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Understanding Patterns

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Patterns of symptomatic vines are an important piece of information needed to solve the cause of poor vineyard growth.

An easy way to identify patterns is the use of aerial imagery (Figure 1) to survey your vineyard. Aerial imagery has improved tremendously and can help detect differences in vine growth, soil and irrigation issues, pest or disease problems and more. Accessing aerial imagery is as easy as using your own drone to capture footage or hiring a licensed pilot or company to take photos and video for you.

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Data from aerial imagery can also be transformed into helpful indices such as NDVI, and specialized sensors such as thermal or hyperspectral images provide additional data for assessing vineyard condition. Aerial imagery can help you track irrigation problems or general vine health throughout the season by outlining patterns based on vine growth and leaf condition.

Figure 1. Aerial vineyard view taken in 1985 showing sand streaks that produce weaker vines that often produce lower yields than the other parts of the vineyard. Aerial imagery taken today can be taken with specialized sensors that provide additional data for assessing a vineyards condition (photo by L. Progressive Crop Consultant Ads With No Banners 08132021 RRR.pdf 1 8/13/2021 Peter Christensen.)

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Continued from Page 29 yard on foot or with an ATV allows for a closer look at low-vigor areas, which may give you management clues that can complement aerial imagery. Patterns of poor growth found on vineyard edges, down rows or in small patches are sometimes easily solved when those areas are visited on foot. For example, Figure 2 shows multiple vines within a row that are weak or dead. At first glance, a disease might be suggested as the cause of poor growth and death.

But after further investigation from top to bottom, it was found that the base of the vine and root system had been chewed and girdled by meadow voles. Once the problem was identified, a management plan was implemented, which ended additional vine deaths.

Symptom Diagnosis

Diagnosing abnormal growth symptoms is somewhat of an art and a science. When called to identify the cause of poor or unusual growth, a vineyard diagnostician must consider all the scenarios that might be causing symptoms.

Table 2. Some likely causes and symptoms* of certain pa>erns found in vineyards.

VINEYARD PATTERN

LIKELY CAUSES*

LIKELY SYMPTOMS

Down the vine row

Irrigation Gopher damage Mechanical injury

Poor growth, yellowing leaves Weak or dead vines Weak or dead vines, poor growth, leaf color

Irregular, related to soil type

Nutrient deficiencies Salinity or alkali Soil compaction Moisture: deficiencies or excess Rootstock differences Nematodes or phylloxera Plant diseases

Various leaf colors and foliar patterns Marginal leaf burn, chlorosis Weak vine growth, chlorosis Chlorosis Varying vine growth & foliar patterns Weak vine growth, chlorosis Weak vine growth, varying foliar patterns

Irregular, related to abiotic causes

Inadequate irrigation Leveled ground; topsoil moved Chemical drift Mechanical injury; i.e. tractor blight Girdling; i.e. grafting tape

Poor growth, chlorosis or reddening Weak vines, poor canopy growth Spotting on leaves and fruit, dead vines Weak or dead vines, poor growth, color Weak, poor growth, dead vine

Seasonal impacts

Temperature related Frost or freeze events Cool weather post budbreak Hot weather

Dead leaves, shoots, or vines Spring fever symptoms Burnt leaves, dead vines

*Specific pest, disease and nutrient deficiency symptoms have not been included in this table.

Table 2. Some likely causes and symptoms* of certain patterns found in vineyards.

A methodical approach that results in an accurate diagnosis is important since time is of the essence when considering management strategies to minimize yield and quality losses.

Variety, rootstock, vineyard age, soil type(s) and depths, irrigation methods and timings, nutrition, common pests, diseases, climate and many other factors must be considered. A systematic approach must be taken to identify the primary cause that includes symptoms found in the vineyard, including related reports, photos and laboratory analyses.

Diagnosing grape pests or diseases can be easier than abiotic causes since three factors need to be present: the pest or disease, grape (i.e., host) and favorable environmental conditions.

as causes, a diagnostician must spend time reviewing reports, lab analysis and walking the vineyard looking for patterns and additional clues. A shovel, shears, soil probe, and a lot of time will be needed to narrow down the cause.

Finding a trusted advisor can also be daunting because there’s often a cost associated with hiring someone. Here are some considerations for finding and hiring a CCA, PCA, private consultant or agricultural forensic consultant.

Often, the lack of optimal climatic conditions do not allow for pest or disease outbreaks. In contrast, the cause of abiotic symptoms is more difficult to identify and costs time and resources. Once pests or diseases have been eliminated

Solving vineyard problems that are negatively impacting yield and quality can be daunting, especially when you are working alone. At some point, you may need to consult with an expert.

Finding a Trusted Advisor

Before hiring someone, clear goals need to be identified so they can be shared with a prospective consultant. First, what are the yield and quality goals for the vineyard and are they being impacted by the problem? Are you trying to

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Figure 2. Multiple vines in a row displaying discolored foliage that looks to represent a virus infection. However, a closer look (photo inset) reveals that the base of the trunk and larger structural roots have been chewed on by meadow voles. As a result of the chewing, the vines were girdled and displayed reddening symptoms, which is similar to a virus infection (photo courtesy S. Vasquez.)

January / February 2022

determine what has reduced vineyard performance or what has killed vines?

Second, do you want solutions to end the loss of vines and stop the yield decline? Finally, do they have the experience needed to help you solve your vineyard problem? Are they focused on your success or are they just interested in selling you something that will “correct” the problem? If it is a challenging problem, do you feel confident that they will tell you the truth if they don’t know what the cause of poor growth is in your vineyard? If you don’t already have a trusted CCA or PCA, it may take some time to interview a consultant that you feel confident will help you and have your success in mind.

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Harvesting and handling California Table Grapes for Market, University of California Bulletin 1913

Books Grape Pest Management, 3rd Ed. University of California Publication-ANR 3343

Compendium of Grape Diseases, Disorders, and Pests, 2nd Ed. APS Press Comments about this article? We want to hear from you. Feel free to email us at article@jcsmarketinginc.com

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Resources

There are many useful resources available to help figure out the cause of peculiar vineyard symptoms. Books, websites, blogs and webinars are great sources of information for starting your investigation. However, be mindful that these types of resources may be specific to a location, climate, variety, production system, etc. that may be different from your situation. If your vineyard health issue needs immediate attention, consider contacting a UCCE Farm Advisor, Extension Specialist, CCA or PCA that specializes in grape production. If your grapevine health issue is caused by a pest or disease, experts can help develop a management program that minimizes crop damage.

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Internet and App resources: UC Integrated Pest Management: http:// ipm.ucanr.edu/index.html Vineyard Advisor app: Android: https://play.google.com/store/ apps/details?id=edu.tamu.agrilife.VineyardAdvisorApp Apple: https://itunes.apple.com/us/app/ vineyard-advisor/id1187381601?mt=8 SoilWeb app: https://play.google.com/ store/apps/details?id=com.casoilre-

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A New Biocontrol Approach for the Reduction of Pierce’s Disease in Vineyards

XylPhi-PD injections contain bacteria-killing viruses that limit losses associated with PD.

By ANIKA KINKHABWALA | Ph.D., Principal Scientist, A&P Inphatec

T

hough vineyard managers continually face many challenges to optimal productivity, Pierce’s Disease (PD) represents a particularly formidable threat due to limited options for effective prevention and control.

PD is a degenerative, deadly and costly disease of grapevines caused by Xylella fastidiosa subsp. fastidiosa (Xff) bacteria, Gram-negative rod-shaped microbes with characteristic large pili. Though hosted by many plant species, these bacteria are easily spread to grapevines by insect vectors such as blue-green and glassy-winged sharpshooters (Figure 1, see page 41). Xff colonize the gut of sharpshooters and are transmitted to grapevines as the insects feed on vines.

Figure 2. XylPhi-PD 100-mL vial (photo courtesy Inphatec.)

Pierce’s Disease: A Major Threat

Once inside a grapevine, Xff bacteria impede the normal function of xylem tissue (transport of water and nutrients ‘up’ from roots to stems and leaves.)

This damage induces the characteristic chlorosis and scorching of leaves, causing early symptoms of PD which mimic water stress. However, the insidious and cumulative damage caused by PD eventually kills entire vines in one to five years.

PD represents a major threat to U.S. wine regions, accounting for widespread economic damage (e.g., roguing and replanting of vines, low fruit production, etc.) and costly deployment of resources aimed at disease moderation. PD has been reported in 28 California counties, covering most of all wine-producing regions. State Extension teams in Texas, Arizona and 32

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Figure 3. Viral bacteriophage particles of XylPhi-PD precisely targeting their bacterial host (photo courtesy Inphatec.)

North Carolina have reported significant outbreaks in 2021. Lost production and vine replacement has been estimated to cost grape producers about $56.1 million annually as of 2014. Further, a 2016 survey of nearly 200 growers and January / February 2022

managers in Napa and Sonoma counties revealed that 73% of respondents identified PD was one of their top three management problems.

Few methods for controlling and

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Figure 1. Blue-green sharpshooter (top) and glassy-winged sharpshooter (bottom), two of the main insect vectors for spread of Xff (photos courtesy Inphatec.)

treating PD have been made available, with efforts historically focused on controlling the sharpshooter vector (e.g., insecticides, trapping, monitoring, inspections) or roguing seriously ill vines (rogue, replant), all of which has achieved only limited success. However, a new option that reduces PD in grapevines is now available.

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New Bacteriophage Injection for PD

XylPhi-PD is a novel, OMRI-listed, biological treatment for PD, a cost-effective break-through technology developed exclusively for viticulture by A&P Inphatec (Figure 2, see page 32).

XylPhi-PD contains a cocktail of viral bacteriophages (Figure 3, see page 32) that are injected into a grapevine (‘bacteriophage’ are bacteria-killing viruses that selectively infect bacteria but do not infect the eukaryotic cells of plants or animals.) These virus particles enter and destroy Xff bacteria, thus limiting bacterial growth and the xylem-clogging damage to the plant. Hundreds of phage particles can be manufactured inside an Xff bacterial cell after infection by a single phage particle. The Xff

Zinc-Shotgun® is a fertilizer that focuses on micronutrients to satisfy needs of customers seeking high zinc with manganese, iron and copper. The micronutrients are completely chelated with natural organic acids, amino acids, and carbohydrates that are readily bio-degradable and supply energy to the plant and soil microflora. Many soils are low in zinc and also require other micronutrients for the growth of good crops.

Complete, organically complexed micronutrient package containing essential elements to improve plant health and growth.

The nutrients are readily absorbed by the plant for a faster response.

Designed to be applied both by foliar application and fertigation practices and is also effective when applied directly to the soil.

Organically complexed with plant based amino acids, organic acids, and complexed polysaccharides.

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Continued from Page 33

Design: 30 Chardonnay and 30 Cabernet Sauvignon vines in an area with high natural PD pressure randomly assigned to each of the four groups.

Vines treated zero, one, two or three times with XylPhi-PD during the summer.

bacterial cell eventually dies and releases all newly created phage particles to seek and destroy more Xff bacterial cells (Figure 4).

XylPhi-PD applications are made by injection of the product into the vascular system of grapevines. Applications are made directly into the active xylem tissue of the plant using a pressurized injection device, the Xyleject Injection System (Figure 5).

Results: Disease incidence in the XylPhi-PD-3X group was significantly reduced by 44% (P = 0.047) compared to controls (10% vs 18%). Activity of vectors positive for Xff was high during the trial period.

XylPhi-PD can be flexibly applied as a treatment when disease symptoms appear, as a preventative to protect growing vines, or whenever conditions may lead to disease. As with most any disease situation, disease prevention or treatment early in the disease process provides much better outcomes than treatment of later-stage severe infections. XylPhi-PD is available in 100-mL vials (treats up to 300 mature vines or 600 young vines.) The product has no restricted-entry interval (REI), requires minimal personal protective equipment (PPE) when used in accordance with label directions and is approved for use in organic production (OMRI-listed, Organic Materials Review Institute).

Efficacy Overview

Multiple studies have been conducted to support the development and commercialization of XylPhi-PD, and results have provided much insight regarding the product’s efficacy profile and use strategies. Brief overviews of some of these studies follow: Greenhouse pilot study (Texas A&M, 2014): Design: 30 greenhouse grapevines inoculated with Xff. 15 vines treated with XylPhi-PD once at three weeks post-inoculation, 15 vines received only buffer. Visual symptoms of PD assessed 12 weeks post-inoculation.

Results: XylPhi-PD reduced PD incidence by 87%.

Natural infection field trial (Texas A&M, 2015): 34

Progressive Crop Consultant

Challenge studies, prevention and therapeutic treatment (California University, 2017):

Figure 4. Death and rupture of a bacterial cell, releasing newly created phage particles to seek and destroy more bacterial cells (photo courtesy Inphatec.)

Design: Prevention study – 15 Chardonnay and 15 Cabernet Sauvignon vines treated with XylPhi-PD and then challenged twice with Xff. Therapeutic study – 30 Chardonnay and 30 Cabernet Sauvignon vines/ group challenged twice with Xff and then treated zero, one, two or three times with XylPhi-PD. Results: Prevention – prechallenge XylPhi-PD significantly reduced incidence of PD symptoms by 75% in Cabernet Sauvignon vines (P < 0.10) and 100% in Chardonnay vines (P < 0.05) vs controls.

Therapeutic – three post-challenge XylPhi-PD treatments significantly reduced incidence of PD symptoms by 90% in Cabernet Sauvignon vines (P < 0.05) and 77% in Chardonnay vines (P < 0.10) vs controls.

Multi-year natural infection field trial (2017-20; Sonoma, Calif.): Design: Two groups of healthy Zinfandel vines were tracked in a high PD-pressure, organic vineyard for three seasons (Ridge, Lytton Springs). One group (n=71) received XylPhi-PD three times/summer, while controls (n=94) only received buffer.

Results: After three years of treatment, vines treated with XylPhi-PD show much less PD incidence than control

January / February 2022

Figure 5. Injection of XylPhi-PD into trunk of mature grapevine. Xyleject Injection System (Pulse Biotech, LLC; Lenexa, KS)

vines as assessed by both qPCR (-60%) and visual PD symptoms (-72%). Vines treated with XylPhi-PD also generated higher fruit yields, averaging +1.34 lb/ vine (+21%) more than control vines.

4-site, 3-year, Natural Infection Field Trial (2019-21; Sonoma, Calif.): Design: A three-year, multi-location commercial (Wilbur-Ellis) field study evaluated the efficacy of XylPhi-PD against endemic PD across four sites and three production seasons. The extensive research effort began in 2019 when a study was conducted that involved 400 vines (300 Chardonnay, 100 Pinot Noir) at three Sonoma County commercial wineries with a history of PD (one winery had two test fields) (Figure 6). All four commercial vineyards were historically high PD sites, and despite continual roguing and insecticide use in the past, a persistent reservoir of Xff remained in the vineyards from previous infection cycles.

Thus, each site included vines with both early-stage and chronic/severe PD.

Xyleject Injection System (Pulse Biotech, LLC; Lenexa, KS)

Figure 6. Locations of four sites used for three-year field study.

Figure 7. Study design and timeline for three-year multi-site field study.

Vines were randomly selected in treatment blocks at each site and assigned to either of two treatment groups as follows: -Control (untreated): n=200 (50/site); -XylPhi-PD: three treatments (Jun/Jul/ Aug); 80 µL of XylPhi-PD injected twice in the trunk and once in each cordon (4 to 6 injections = one treatment); n=200 (50/site).

Six petioles from each vine were collected in September for analysis by quantitative polymerase chain reaction (qPCR) and confirmation of Xff infection. All study vines were also visually assessed by trained observers for PD development. Insect traps were placed at each study site in an attempt

to monitor vector pressure.

A continuation of the same study protocol was followed in 2020 and 2021, allowing two additional seasons of treatments and observations for the same vines/blocks at the same sites/ wineries. In addition, treatments and observations of additional vines were initiated in 2020 at each site (n=50/ site, 200 total), repeated again in 2021 (Figure 7). As a result, three groups emerged from the study for tracking and evaluation: Vines with three years of treatment (three-year treated, n=200) Vines with two years of treatment (twoyear treated, n=200)

Non-treated controls (n=200)

Results

Comparative outcomes for vines qPCR-positive for Xff are summarized in Figure 8, see page 36. Under the conditions of only mild to moderate PD pressure and low vector populations, sequential year-to-year use of XylPhi-PD generated impressive results.

Incidence of Xff positivity fell 24% and 45%, respectively, for vines receiving two or three years of treatment compared to untreated controls. Improvement in the three-year group was significant (P < 0.05) vs controls. The few vines remaining Xff-positive in the two- and three-year groups were chronic infections that would be rogued.

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Figure 8. Vines qPCR-positive for Xff, or vines showing visual signs of PD. Summary of four sites in Sonoma County.

Continued from Page 35 The visual assessment of vines for signs of PD was another important study parameter, and outcomes (Figure 8) were similar to those using qPCR confirmation of infection. Vines treated with XylPhi-PD for two years generated a 27% reduction in visual PD incidence compared to controls, while vines treated for three years showed double the benefit, a significant 54% reduction (P < 0.05) of PD incidence. The similarity of these data with qPCR results suggests that visual assessments can help vineyard managers tangibly gauge the efficacy of XylPhi-PD. Notably, XylPhi-PD continued to protect against PD infections at all four trial sites for the three years of observations, with no new infections detected in the three-year treated group. In contrast, the control group had ~2% to 4% new infections.

Fruit yield was measured at one study site (D) at the study’s conclusion in 2021 (Figure 9, Chardonnay, eight- to ten-year-old vines). Compared to untreated controls, vines in the group treated with XylPhi-PD for three years averaged 5.1 lb (+17%) more fruit per vine than controls. Vines treated for two years were intermediate (3.1 lb, +10% more fruit vs controls).

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Usage Recommendations

XylPhi-PD can be applied as a preventive treatment to protect growing vines, as a therapeutic treatment when disease symptoms become visible, or anytime production conditions may lead to disease pressure.

Locations selected for application of XylPhi-PD can be Figure 9. Average fruit yield for Chardonnay vines at one site based on the age (D). of the plant, the pruning style and/ or the training system utilized for the mended during each growing season at plant. The product is to be injected into near-monthly intervals (Figure 11, see the active xylem vascular tissue above page 37). This frequency of application ground level. Two examples of injection has been demonstrated to provide optistrategies appear in Figure 10, see page mal PD control under various levels of 37. On established vines, for example, PD pressure. The volume of XylPhi-PD one or two injections can be applied in administered can also vary depending the trunk with an additional injection on the age of plants being treated and in each cordon or spur. For young, rePD pressure. cently planted or radically pruned vines, apply two or three injections into shoot, The quantity of XylPhi-PD used in two to six inches above the ground. For XylPhi-PD treatment programs can all scenarios, the total number of injecvary based on the number of injections/ tions administered to a vine define one vine, the concentration of product/in‘application’ of XylPhi-PD. jection and the number of applications/ year. Growers and PCAs have options For most production situations (mediand flexibility to match doses and the um to high PD pressure), two or three number of applications to their specific applications of XylPhi-PD are recomconditions and risks. Table 1 summaJanuary / February 2022

Table 1. XylPhi-PD dosage options and number of applications per vial (100 mL).

Figure 10. Recommended XylPhi-PD injection locations.

Figure 11. Examples of XylPhi-PD application programs involving medium to high PD pressure.

rizes some of these options and their impact on the amount of XylPhi-PD used and number of vines treated per 100-mL vial.

Treatment Strategies for PD Management

Several recommendations for managing PD across an entire vineyard have

emerged from field use experience with XylPhi-PD. These recommendations largely depend on the scope and distribution of PD in a particular vineyard or block.

As usual, vines demonstrating severe and/or chronic PD infection should be

Continued on Page 38 January / February 2022

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Continued from Page 37 rogued per IPM protocols.

Vines appropriate for XylPhi-PD treatment should be identified.

These appropriate vines should be treated with recommended doses of XylPhi-PD at two or three seasonal applications as needed for mature vines or replants (per label directions).

It is the second step, identifying which vines to treat, that can sometimes pose a quandary for production managers.

To help in this process, three strategic options can offer direction for developing a customized plan for vineyard-wide PD management. Based on evaluations regarding the spread of infection and site specifics for a particular vineyard or block, a treatment strategy can be selected from the following (Figure 12): Targeted buffer zone: Treat all vines in any defined area with high PD activity (>2% symptomatic vines) and/or vector activity, such as near a riparian area.

Precision spot: In large areas with few symptomatic vines, mark/identify affected vines and treat only them and their immediate surrounding neighbors.

Entire block: In large areas with multiple scattered symptomatic vines, in the presence of vectors, full-block treatment is needed to reduce overall PD pressure.

In addition to these three options, the duration of treatment (multiple years) is also a critical consideration. As discussed earlier, field studies have demonstrated the cumulative value of consistent treatment with XylPhi-PD over multiple years for reducing the level of PD in a vineyard or block, even when under low PD pressure. As always, prevention of severe, chronic disease is the best approach, and consistent long-duration use of XylPhi-PD appears to substantially diminish PD 38

Progressive Crop Consultant

Figure 12. Strategies and options for treating areas within vineyards or blocks.

progression and pressure in vineyards.

Identifying PD

Some of the visual signs of PD damage are presented in Figure 13, including characteristic chlorosis (leaf scorching), irregular lignification and berry shriveling, and ‘matchstick’ petioles. In general, PD is likely present in a vineyard if the following four symptoms are observed late in the season: • Leaves scald in concentric rings or in sections • Leaf blades abscise, leaving petioles attached to the cane • Bark matures irregularly • Fruit clusters shrivel or raisin January / February 2022

However, damage caused by various other diseases, water stress, pests or nutrient deficiencies/imbalances may produce symptoms similar to those caused by PD. Therefore, PD must be laboratory confirmed by detection of Xff by qPCR testing on late-season/fall petioles (e.g., UC Davis Foundation Plant Services, Texas Plant Disease Diagnostic Lab, Arizona Plant Diagnostic Network).

Conclusions

PD is an extremely challenging problem for wine producers and their consultants. A biological control approach with XylPhi-PD offers a fresh opportunity to help manage PD and limit losses associated with the disease. In multiple studies, XylPhi-PD treatment of diverse wine varietals prompted reductions

Figure 13. Examples of visual damages caused by PD (photos courtesy Inphatec.)

in PD incidence and/or severity under conditions of both natural and challenge infection with Xff. These favorable outcomes distinguish XylPhi-PD as a targeted and cost-effective strategy for effectively protecting valuable vineyards against PD. Always read and follow product label directions. Not registered in all states. EPA Reg. No. 93909-1. Operators of injector must undergo training and be certified by Pulse and must follow instructions in device manual. XylPhi-PD is a trademark of A&P Inphatec. Xyleject is a trademark of Pulse Biotech.

Field trial report, 2020. Ridge Lytton Springs/A&P Inphatec.

Data on file 2020 Field Trial Results: XylPhi-PD for Control of Pierce’s Disease. A&P Inphatec Technical Bulletin. https://inphatec. com/wp-content/uploaXffds/XylPhi-2020-field-trials-1.pdf Comments about this article? We want to hear from you. Feel free to email us at article@jcsmarketinginc.com

Beat the Heat & Care for Your Crops with:

References

Anti-Stress 550

Pierce’s disease research updates. California Department of Food and Agriculture. http://piercesdisease.cdfa.ca.gov (accessed December 2021).

Pierce’s Disease. California Department of Food and Agriculture. https://www.cdfa.ca.gov/pdcp/Pierce’s_Disease.html (accessed December 2021).

Tumber KP, Alston JM. Pierce’s disease costs California $104 million per year. California Agriculture 2014; 68(1):20-29.

Evaluating Potential Shifts in Pierce’s Disease epidemiology. California Department of Food and Agriculture. https:// static.cdfa.ca.gov/PiercesDisease/reports/2019/CDFA%20150453-SA%20Final%20Report.pdf (accessed December 2021).

Ahern SJ, Das M, Bhowmick TS, Young R, Gonzalez CF. Characterization of novel virulent broad-host-range phages of Xylella fastidiosa and Xanthomonas. J Bacteriology 2014; 196:459-471.

Texas A&M Research Progress Report, 2014. Data on file.

Texas A&M Research Progress Report, 2016. Data on file.

CA 2017 Field Trial Progress Report, 2017. Data on file.

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Delayed Spring Growth and Grapevine Production During Drought

By GEORGE ZHUANG | UCCE Viticulture Farm Advisor, Fresno County and MATTHEW FIDELIBUS | Extension Viticulturist, UC Davis

A

fter 2021 grapevine budbreak, we received many calls about dead spurs, delayed bud break, stunted shoot growth and poor fruit set. In Fresno County, some severely impacted vineyards suffered a substantial yield loss. In many cases, the problem was delayed spring growth (DSG), and the classic vine symptoms include:

•

Delayed and erratic bud break

•

Stunted shoot growth

•

Excessive berry shatter and poor fruit set

the minimal temperature of the 2020 winter might be lower than the last five years’ average according to the CIMIS station data, the DSG’s occurrence and severity varied significantly across

The situation was apparently spread across different grape growing regions in California, and UC Davis Department of Viticulture and Enology held a virtual grower meeting to discuss it (the recorded presentation can be found on the UC Davis AggieVideo website.)

Delayed Spring Growth

Grapevine DSG is associated with insufficient rehydration of the vines and may be due to vascular tissue injury, insufficient carbohydrate reserves, excessively dry soil over winter or some combination of these factors. Symptoms can result in significant yield loss and permanent vine damage, resulting in economic hardship for growers. Some vine DSG symptoms are similar to other pest/disease symptoms (e.g., vine trunk disease or soil pests like nematodes/phylloxera.) However, most vineyards we visited had little or no sign of trunk disease or soil pests. Several factors this past fall and winter contributed to widely observed and severe vineyard DSG symptoms: •

Ongoing drought and increasingly dry soils, especially over winter in vineyards which were not sufficiently irrigated postharvest, or during winter

•

Warmer than normal fall temperatures, including a particularly warm October

•

A sudden freeze in early November

According to CIMIS station data at Five Points, October 2020 was warmer than the last five years’ average and followed a sudden freeze event in November (Figure 1), and warmer-than-normal autumn is a risk factor for DSG. In addition to the November freeze event, October and November 2020 were mostly dry, and a drier autumn could make the freeze worse. Even though 40

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Top left: delayed and erratic bud break from DSG vines; top right: stunted shoot growth after bud break from DSG vines; bottom left: Inflorescence bloom on a weak canopy; bottom right: Excessive berry shatter and poor fruit set from DSG vines (all photos courtesy G. Zhuang.)

Top left: Selma Pete dead cordon from botryosphaeria canker; top right: Trunk discoloration and pie shape canker on Selma Pete from botryosphaeria canker; Bottom left: Grapevine decline from nematodes; bottom right: Grapevine root galls from root-knot nematodes.

vineyards in Fresno County and other parts of California.

Daily Minimal Temperature (F )

Also, vineyard management, particularly postharvest and winter irrigation, could make a big difference on the results of DSG even if the ambient weather condition was similar. The

2020-2021 CIMIS Station at Five Points

70

To make matters worse, Fresno County saw much less precipitation during the months of November and December 2020 than the last 20 years’ average (Figure 3). These drier months might offer the perfect conditions for DSG. Although precipitation amount was normal in January 2021, February was yet another dry month in comparison to historical averages. Lack of soil moisture before bud break is another major risk factor for DSG.

60 50 40 30 20 Sep

Oct

Nov

Dec

Jan

Feb

Mar

Apr

Daily Minimal Temperature (F )

Figure 1. Daily minimal temperature from September 2020 to April 2021 at CIMIS Station near Five Points, Calif.

-

2020-2021 UC IPM Weather Stations at Fresno Co. 70 2020-2021 CIMIS Station at Five Points

Vineyard conditions should be considered to avoid DSG and possible cold damage:

50 40

• Abiotic or biotic stressed vines (e.g., severe water stress and overcrop, nutrient deficiency, pest/disease)

30 20 10 Sep

-

Precipitation (in)

-

4

• Young vines Oct

Nov

Jan Dec Date of year

Feb

Mar

Apr

Precipitation From October to March

3

2 1 0

Grapevine winter freeze damage and DSG have similar symptoms and can be difficult to differentiate. Winter cold damage or freeze injury damages vascular tissues and can thus interfere with water, carbohydrate and mineral translocation, causing symptoms similar to DSG. A lack of soil moisture can impair vine rehydration, making vines suffer water stress and causing DSG symptoms directly. Additionally, vines might be more vulnerable to cold injury even though the minimal temperature in the past winter, such as 25 degrees F at Sand Ranch, might not cause significant freeze damage on most Vinifera grapes.

Maintain Vine Health

60

Figure 2. Daily minimal temperature from September 2020 to April 2021 at five UC IPM weather stations in Fresno County.

-

geographic location as well as vineyard microclimate can sometimes mean quite different consequences in the face of freeze damage. Figure 2 illustrates the variation of daily minimum temperatures from five locations in Fresno County. Typically, vineyards located on the west side had a lower minimal temperature and suffered more freeze damage than vineyards located on the east side. Sand Ranch in particular had the lowest daily minimum temperature among five locations from October to March.

Oct

Nov

Jan Dec Month

Feb

Mar

Figure 3. Monthly precipitation from October 2020 to March 2021 in Fresno County.

• Late ripening and cold tender varieties •

Certain rootstocks, including Freedom and Harmony

• Insufficient soil moisture during the dormant period (e.g., October to March in the San Joaquin Valley) Generally, maintain vine health over the growing season and assess soil moisture as the vines enter dormancy, watering if needed. Too many clusters with not enough leaf area can weaken the vines and deplete the trunk and root’s carbon reserves, which are needed to maintain respiration over winter, help prevent freezing and nourish the vines as they regrow in the spring.

To maintain a functional vine canopy, irrigate as necessary to support photosynthesis without stimulating excessive growth.

If pests or diseases are present in the vineyard, such as powdery mildew, nematodes, grapevine trunk disease, virus, mites and leaf hopper, a good assessment of canopy health is important.

Continued on Page 42 January / February 2022

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41

Continued from Page 41 Grapevines with severe defoliation or small canopies will be of great concern, and management should focus on better addressing pest and disease problems to avoid early defoliation.

A young vine has its inherent nature of vulnerability due to a lack of sufficient carbon reserves. Therefore, severe water stress and overcropping should be avoided, and irrigating the soil before a freeze event (e.g., late October and early November) can be greatly beneficial to provide heat protection for young vines.

This past spring, we noticed some susceptible varieties might suffer greater damage from DSG, and that has been consistent with the reports from other growers. Chardonnay and Pinot gris have been reported frequently on DSG, although both varieties are also susceptible to winter freeze.

Top Left: soil auger. Top Right: Soil water potential sensor. Bottom: Soil water volumetric sensor. Many tools can provide great benefits for assessing soil moisture and help growers determine whether or not to irrigate.

WE’RE BACK WITH

PECAN

DAY

Rootstock can also play an important role in DSG. Certain rootstocks (e.g., 5BB and Freedom) are more susceptible to DSG than others (e.g., 1103 P), according to results of UCCE rootstock field trials in different growing regions of California. Thus, growers who have the susceptible rootstock might want to take extra care of the vines, such as irrigating the soil during the dry winter, so that the risk

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20% yield loss has been reported for some vineyards in Fresno County in 2021, and DSG might play a large role in it, although the record summer heat and seasonal variation could also result in loss. Finally, the consequence of DSG on Fresno vineyards varied greatly. Some vineyards appeared to be significantly stunted after budbreak and later fully recovered due to irrigation. Some vineyards might suffer multi-year yield loss due to the weakened canopy and few desired canes to prune.

Petite Verdot on 5BB rootstock at front and Cabernet Sauvignon on 1103 P rootstock in back.

of potential DSG might be minimized.

Manage Soil Moisture

Last but not least, lack of soil moisture might be the most important yet manageable factor contributing to most DSG farm calls. As discussed previously, drier October, November, December and January months posed a great risk of DSG as well as inhibited rehydration of the vines, which can also lead to a greater risk of freeze damage. However, water availability during the drought years might be significantly reduced or expensive.

Therefore, irrigation during the drought years can become the dilemma. Growers need to balance the cost and reward of irrigation vs. no irrigation during drought years. Greater than

• Tank Mix Compatible • Foliar or Soil Applied

In the face of upcoming potential drought, growers can use multiple tools to reduce or eliminate the effect of soil moisture deficit. Many tools (e.g., shovels, soil augers, moisture sensors) can provide great benefits for assessing soil moisture and help growers determine whether or not to irrigate. Weather stations can also provide great amounts of information regarding the minimal ambient temperature as well as the amount of local precipitation, since temperature and precipitation can vary greatly from one vineyard to another. UC IPM has seven weather stations in Fresno County and one station in Madera County in cooperators’ vineyards, and those stations can offer both temperature and precipitation amount, serving the growers whose properties are nearby the station.

Comments about this article? We want to hear from you. Feel free to email us at article@jcsmarketinginc.com

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ALWAYS READ AND FOLLOW PESTICIDE LABEL DIRECTIONS. Not all products are approved and registered in all states and may be subject to use restrictions. The distribution, sale, or use of an unregistered pesticide is a violation of federal and/or state law and is strictly prohibited. Check with your local dealer or representative for the product registration status in your state. Bayer, Bayer Cross, and Movento ® are registered trademarks of Bayer Group. For additional product information, call toll-free 1-866-99-BAYER (1-866-992-2937) or visit our website at www.BayerCropScience.us.

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