Design of Biological clock with Biosensor for early stage detection of cancer R.Laik Researcher
Abstract
- Human Cell Cycle gives birth to new cells hence the cell monitoring device has the ability to monitor the cell damage, repairing system this can be achieved with Biological clock with Biosensor can detect cancer stages their signaling molecule at the check point of cell cycle at G1 Phase, will determine the regulation and control of the cell cycle, in the cancer pathway and detect the damage, Cell cycle monitoring is done by Bioinformatics Reactome server.All of our cellcycle pattern required for particular function is regulated by hormones, with the help of Signal transduction pathway, we can control and monitor the system error, in any type of Disease, but here i want to focus only on cancer signaling pathway and its system, expressed /repressed by biological logic its equivalent Boolean expression working with hormonal expression.
Keywords : - Cell cycle, Biological clock, Cancer pathway, Repairing system, Reactome server. Types of Cell – cell communication pathway are involved 1.
Signal Transduction Pathways
a) Paracrine signaling
2.
Second Messenger
Hypothalamus is the center of the brain which is the controller of our Neuron, Suns light falls on the retinal system stimulates the Psycho motor neuron, function to regulate the biological clock at different point of time in our daily regulate the biological clock at different point of time in our daily life style and hence health management.
Biological Clock
Morning Light Stimulates SCN (Supra chiasmatic Nucleus) Secretes Melatonin Hormone ( Which peaks at night) & come down at morning again. This Clock gives the importance about the cycle of our life style pattern which starts from the early morning, Fooding habit this controls the cell cycle but if any change in timing of the life style pattern or the fooding habit occurs, there could be a error in the cells growth pattern and hence the control of the normal cell from cancer cell will show the difference. This can be observed in the pathological specimens. Biological clock follows this pattern in our life style, every part of this daily habit is sensitive to our daily clock system, which is maintained from Early morning to Night. starts from Cleanliness, Purity of our Soul associated with Yoga.Pranayam
makes and refresh the clock of our system, with inhalation of Oxygen, formation Of new Blood cells, repair of the cell cycle, this can be very well observed at the injury site of the wound healing process, Clotting time and the factors responsible for clot, is important for extra loss of Blood.
Menstrual Cycle in case of female is the regular periodic clock at any week of a month. 1. Standard daily routine pattern 2. Life style pattern of the normal human being 3. Pattern of the human being under clinical observation at hospital
A) Early morning wake up B) Freshness with tooth paste cleaning C) Going to toilet, Lavatory(Early morning) D) Bathing E) Doing Physical exercise,Yoga (Pranayam & Byayam) F) Have a Fruits
G) Heavy break fast is important for high efficient work. H) Heavy Dinner ( lead to laziness, late awake at Morning) I) Early night sleep (Sleep-awake cycle regulated by hormonal switch) Any changes in the life style pattern in our daily routine can affect the cell cycle regulation in controlling the Biological clock to execute its normal function. Hence the cell leads to abnormal cell cycle can lead to precancerous state of the cell, thus the check points plays a significant role for controlling the biomarker, level thus this Biomarkers detect cancer- which is correlated with biological clock.
OPERON SYSTEM
BOOLEAN LOGIC SYSTEM
Genetic Switch (On / Off)
Logic Expression 1 0r 0
Logic 1 is switched on by Promoter, 0 Logic means Switched off by repression system
Depending on the behavior of the cell cycle regulation and the mode of Operon pathways, there are different switch system in Biological circuit the expression of the Biomarker is controlled by regulon, stimulator and modulator. Neural Clock Neuron Synapse system which has inhibitory function and the signal of neuron system is amplified with SCC (Signal conditioning circuit.
circuit). This system monitors the behavior of biological clock of our sleeping and awaking pattern.
Biological Circuit This pathway is important cellular Circuit system which monitors the cancer genetic switch the apoptotic factor, how the regulations of P53gene occurs is shown here cdk4 phosphorylation occurs this is signaling Rb Protein passage in cell cycle. This is a very significant pathway for cancer monitoring its progression to advanced stage of cancer, specially for Blood cancer patients leukemia, Diabetes is the early stage Of Cancer . The patients symptoms like Hemoglobin affinity for Oxygen increases due to the mutation of this P53. Hemolytic Anaemia. This is a genetic sequence of Cyclin dependent kinase, it is the significant machinery, which controls and regulates the cell cycle machinery, at each check point of cell division, through Phosphorylation and dephosphorylation, the machinery is regulated from G1 S G2 M (Dephosphorylation stops the cell cycle at check point to proceed for cell division.
>gi|986878|gb|L47233.1|HUMCIP1WAG Homo sapiens cyclin-dependent kinase (CIP1/WAF1) mRNA, 3' end, with a cancer predisposing mutation in th >gi|986878|gb|L47233.1|HUMCIP1WAG Homo sapiens cyclin-dependent kinase (CIP1/WAF1) mRNA, 3' end, with a cancer predisposing mutation in the 3' UTR GCCGGAGCTGGGCGCGGATTCGCCGAGGCACCGAGGCGGATGTCCGTCAGAACCCATGCGGCAGCAAGGCCTGCCGC CGCCTCTTCGGCCCAGTGGACAGCGAGCAGCTGAGCCGCGACTGTGATGCGCTAATGGCGGGCTGCATCCAGGAGGC CCGTGAGCGATGGAACTTCGACTTTGTCACCGAGACACCACTGGAGGGTGACTTCGCCTGGGAGCGTGTGCGGGGCC TTGGCCTGCCCAAGCTCTACCTTCCCACGGGGCCCCGGCGAGGCCGGGATGAGTTGGGAGGAGGCAGGCGGCCTGGC ACCTCACCTGCTCTGCTGCAGGGGACAGCAGAGGAAGACCATGTGGACCTGTCACTGTCTTGTACCCTTGTGCCTCG CTCAGGGGAGCAGGCTGAAGGGTCCCCA
>gi|237829|gb|S63157.1| p53 {point mutation} [human, gastric cancer metastasis, Genomic Mutant, 15 nt]
ATGAACTGGAGGCCC ATGAACTGGAGGCCC HEX = 415447414143544747414747434343 BINARY = 010000010101010001000111010000010100000101000011010101000100011101000111010000010100 011101000111010000110100001101000011 A = 41 = 01000001 C = 43 = 01000011 G = 47 = 01000111 T = 54 = 01010100
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