ISAT2015 Book of Abstracts by nutthakorn war

2nd International Symposium on

Agricultural Technology Global Agriculture Trends for Sustainability

July 1-3, 2015 A-One The Royal Cruise Hotel Pattaya, Thailand

BOOK OF ABSTRACTS

conf.kmitl.ac.th/isat2015 Organized by Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang (KMITL)

Major Sponsors

ISAT2015 Book of Abstracts

2 International Symposium on Agricultural Technology (ISAT2015)

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Advisory Committee Prof.Dr. Suchatvee Suwansawat Assoc.Prof.Dr.Chamroon Laosinwattana Prof.Dr.Julian Wiseman

Committee

Prof.Dr.Gray Williams

July 1-3, 2015

Prof.Dr.Metha Wanapat

President, King Mongkut's Institute of Technology Ladkrabang (KMITL) Executive Vice President for Research and Innovation, KMITL School of Bioscience, University of Nottingham, United Kingdom School of Biological Science, University of Hongkong, Sar China Director, Tropical Feed Resource Research and Development Center, Khon Kaen University, Thailand

Steering Committee Assoc.Prof. Sakchai Choochote Assoc.Prof.Srisakul Vorachantra Asst.Prof.Dr.Paveena Taweekijakarn Asst.Prof.Peerachai Kullachai Asst.Prof.Dr.Kanya Jirajaroenrat Asst.Prof.Dr.Nutthakorn Songkram Asst. Prof.Dr.Ammorn Insung Asst.Prof.Dr.Monthon Ganmanee Asst.Prof.Dr.Thamrong Mekhora Ms.Sumnao Pattararatnant

KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL

Chairman

KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL

Chairman

Secretary

Organizing Committee Asst.Prof.Dr.Monthon Ganmanee Assoc.Prof.Dr.Tanimnun Jaenaksorn Assoc.Prof.Dr.Chamroon Laosinwattana Asst.Prof.Dr.Paveena Taweekijakarn Asst.Prof.Dr.Kanjana Saetiew Asst. Prof.Dr.Ammorn Insung Asst.Prof.Dr.Nutthakorn Songkram Asst.Prof.Dr.Nittaya Phakamas Ms.Sumnao Pattararatnant Asst.Prof.Dr.Kanya Jirajaroenrat

Secretary

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2 International Symposium on Agricultural Technology (ISAT2015)

Committee

Scientific Committee Assoc.Prof.Dr.Tanimnun Jaenaksorn Assoc.Prof.Dr.Chamroon Laosinwattana Assoc.Prof.Dr.Ronachai Sitthigripong Assoc.Prof.Dr.Tippawan Limunggura Assoc.Prof.Dr.Suneerat Ruangsomboon Asst.Prof.Dr.Kanjana Saetiew Asst. Prof.Dr.Ammorn Insung Asst.Prof.Dr. Teerawat Sarutayophat Asst.Prof.Dr.Nittaya Phakamas Dr. Nonglak Parinthawong Asst.Prof.Dr.Lampan Khurnpoon Dr. Sukunya Yampracha

Chairman

Secretary

International Scientific Committee Prof.Dr.Julian Wiseman Prof.Dr.Gray Williams Prof.Dr.Metha Wanapat

Prof.Dr.Hideo Ishii Prof.Dr. Abdul Salam Babji

Assoc.Prof.Dr. Rajeev Bhat

Assoc.Prof.Dr. Janine Croser Dr.Thierry Tran

School of Bioscience, University of Nottingham, United Kingdom School of Biological Science, University of Hongkong, Sar China Director, Tropical Feed Resource Research and Development Center, Khon Kaen University, Thailand School of Agricultural Regional Vitalization, Kibi International University, Japan School of Chemical Science and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM) Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia Centre for Plant Genetics and Breeding, The University of Western Australia, Australia French Agricultural Research Centre for International Development (CIRAD), France

Ceremony and Reception Committee Asst.Prof.Dr.Kanya Jirajaroenrat Asst.Prof.Dr. Sarayut Phonpho Asst.Prof.Dr. Montinee Teerarak Dr.Rutcharin Limsupavanich Lect. Dusit Aue-umneoy Assist.Prof.Dr.Komkhae Pilasombut Assist.Prof.Dr.Kanjana Saetiew Dr.Duangkamol Panrositp Thunmatiwat Dr.Suneeporn Suwanmaneepong Miss Montha Suwanrath

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KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL

KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL

Chairman

Secretary July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Registration and Financial Committee

Committee

Asst.Prof.Dr.Nittaya Phakamas Asst.Prof.Dr.Komkhae Pilasombut Pattrarat Teamkao Phatchara Eamkijakarn Mrs.Pacharee Keadchoom Asst.Prof.Dr.Lampan Khurnpoon

July 1-3, 2015

KMITL KMITL KMITL KMITL KMITL KMITL

Chairman

Secretary

Public Relations and Technical Committee Asst.Prof.Dr.Paveena Taweekijakarn Asst.Prof.Dr.Kanok Lertpanich Miss Wilai Punpitanusorn Mr.Apisit Sanla Asst.Prof.Dr.Nutthakorn Songkram

KMITL KMITL KMITL KMITL KMITL

Chairman

KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL KMITL

Chairman

KMITL KMITL KMITL KMITL KMITL KMITL

Chairman

Secretary

Welfare Committee Asst. Prof.Dr.Ammorn Insung Asst.Prof.Peerachai Kullachai Asst.Prof.Dr. Teerawat Sarutayophat Asst.Prof.Dr.Prommart Koohakan Asst. Prof.Pornthiwa Kanyawongha Dr. Nonglak Parinthawong Pattrarat Teamkao Lect. Wanida Duangkongsan Mr.Jarongsak Pumnuan

Secretary

Coordinating Committee Ms.Sumnao Pattararatnant Mrs.Pacharee Keadchoom Mr.Chatre Vijitrotai Buppha Jongput Mr.Panurat Jantup Mrs.Lamead Sumrittisut

Secretary

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2 International Symposium on Agricultural Technology (ISAT2015)

Global Agriculture Trends for Sustainability

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Contents Page

Rationale Report Address Welcoming & Opening Remarks Short Biography of Invited Speakers ISAT2015 Program Scientific Program Keynote and Invited Abstracts Oral Abstracts Poster Abstracts List of Participants Authors Index

July 1-3, 2015

2 3 4 5 13 17 35 47 89 155 159

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2 International Symposium on Agricultural Technology (ISAT2015)

Rationale

The global population is expected to increase from 7 billion today to 9 or 10 billion people by 2050. This situation puts increasing pressures on agriculture to produce much more food, as well as non-food crops. Agricultural practices are currently unsustainable and generate many issues that must be addressed, including over-use of pesticides and fertilizers, land use change, loss of biodiversity, soil erosion, water management for irrigation, management and utilization of crop residues and waste, increasing yields without increasing agricultural surfaces, and so on. Sustainable agriculture which defined as "an integrated system of plant and animal production practices having a site-specific application that will last over the long term" has been accepted worldwide as a significant trend for agricultural practice. This issue offers various positive aspect, for example, satisfy human food, enhance environmental quality and the natural resource base upon which the agricultural economy depends, make the most efficient use of non-renewable resources and on-farm resources and integrate where appropriate, natural biological cycles and controls, sustain the economic viability of farm operations, enhance the quality of life for farmers and society as a whole. However, knowledge gap still remains in some area. Therefore, research is urgently needed to make agricultural practices more sustainable in order to reduce environmental impacts to all production systems, including crops, livestock, fishery and forests. As above mentioned, in this event 2nd International Symposium on Agricultural Technology is organized with aim to promote the exchange of ideas and knowledge to solve current and future problems related to sustainability of agriculture. We will focus on the topic “Global Agriculture Trends for Sustainability” in which consisted of various sessions. As for plenary session, more detailed on “Global Agriculture Trends for Sustainability” and related topics will be focused. Many of oral sessions as well as poster sessions are also organized. We hope that this event will promote the exchange of ideas and knowledge to solve current and future problems related to sustainability of agriculture as well as future and practical prospects concerning self-reliance, moderation, reasonableness, and self immunity for agriculture, and other related issues in different countries.

The 2nd

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(Assistant Professor Dr.Monthon Ganmanee) Chairman Organizing Committee International Symposium on Agricultural Technology (ISAT2015)

July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Report Address On behalf of the Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang (KMITL), I would like to extend my warm welcome and appreciation for your participation at the 2nd International Symposium on Agricultural Technology (ISAT2015). It is the goal of Faculty of Agricultural Technology to be recognized in the region as one of the top fifth Agricultural Science and Technology universities. To fulfill this goal, ISAT was first originated in 2010 hosting by the Faculty of Agricultural Technology, KMITL, jointly with Tokai University, Japan under the theme of the conference “Sufficiency Agriculture”. It was a dual conference with the 16th Asian Agricultural Symposium. For this year, the 2nd International Symposium on Agricultural Technology was organized with the main concept of “Global Agriculture Trends for Sustainability”. This symposium will offer a dynamic platform for scientific research update and knowledge sharing on Agricultural Technology among students and researchers both from local and abroad. This symposium composed a total of 150 participants including honorary guest speakers, faculty members, researchers and students from various universities and institutes in Thailand and from abroad. There are 63 posters and 40 oral research presentations submitted. I hope this delightful opportunity will create a dynamic research network and bring out more collaborated activities between KMITL and other universities or institutes both at national and international levels.

Assoc.Prof. Sakchai Choochote Dean, Faculty of Agricultural Technology King Mongkut’s Institute of Technology Ladkrabang

July 1-3, 2015

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Welcoming & Opening Remarks As global population is expected to increase dramatically, world scarcity of foods and agricultural raw material supplies are well expected. Issues in global sustainability, due to agricultural raw material production to serve this growing population, have been long recognized. But new problems arise and keep changing. Itâ&#x20AC;&#x2122;s necessary for agricultural technologists and scientists to keep their eyes on those changing world issues. On behalf of King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, it is an honor and my great pleasure to welcome all guests and participants to the 2nd International Symposium on Agricultural Technology (ISAT2015). The goal of this symposium is to emphasize how importance for the awareness in continuing and immerging issues of world sustainability. I certainly believe that this conference will provide great motivation for all researchers and graduate students to produce more quality research work. In addition, all activities at the meeting will create an excellent floor for research exchange and collaborations among all institutions, nationally and internationally. With the goal of this conference in mind, I believe it will lead to an advance in agricultural technology not only in Thailand, but also in our international research network. I hope all participants find the poster and oral research presentation, special topics from distinguished guest speakers as well as all conference extra activities delightful and useful for their careers.

Prof.Dr. Suchatvee Suwansawat President King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang

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2 International Symposium on Agricultural Technology (ISAT2015)

Short Biography of Invited Speakers Keynote Speakers "Status of global agriculture and food security situation, and the future trend"

Dr. Hiroyuki KONUMA Assistant Director-General and FAO Regional Representative for Asia and the Pacific FAO Regional Office for Asia and the Pacific Phra Athit Road, Phranakorn, Bangkok Thailand Telephone: +66-2-6974000 Fax: +66-2-6974445 E-mail: FAO-RAP@fao.org

Dr. Hiroyuki Konuma, a Japanese national, he has been with FAO for 33 years. He was appointed as the FAO Assistant Director -General and the Regional Representative for Asia and the Pacific in March 2010. During this tenure as the Regional Representative of FAO for Asia and the Pacific, he chaired the Regional UN Thematic Working Group on Poverty and Hunger and coordinated the launch of Zero Hunger Initiative for Asia and the Pacific and the Regional Save Food Campaign in 2013. He promoted the role of FAO in the region and coordinated FAOâ&#x20AC;&#x2122;s experts and programs in the field of agriculture, food security, nutrition, rural development and with an aim to promote regional coordination and collaboration, and promote food and nutrition security towards achievement of Millennium Development Goals and Zero Hunger targets.

July 1-3, 2015

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"Optimizing the use of feed resources for healthy rumen, increased production and mitigating methane"

Professor Dr. Metha WANAPAT Director of the Tropical Feed Resources Research and Development Center (TROFREC), Khon Kaen University, Thailand Department of Animal Science, Faculty of Agriculture, Khan Kaen University, Thailand Email: metha@kku.ac.th

Prof. Dr. Metha Wanapat, has long-term experience for more than 30 years in teaching, research engagement in ruminant nutrition and feeding systems in the Tropics. His research work encompasses a wide range of aspects of feed resources, conventional and non-conventional feed resources both on farms and at industrial practicalities. His current research work have been on establishment of on farm feed resources using food-feed-system approach, strategies of supplementation and manipulation of rumen using dietary means to improve rumen fermentation efficiency, methane mitigation and enhance ruminant productivity.

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2 International Symposium on Agricultural Technology (ISAT2015)

"Food Sustainability - Emerging Challenges In the ASEAN Region (Reality versus the Myth) "

Associate Professor Dr. Rajeev BHAT Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Penang , Malaysia rajeevbhat1304@gmail.com rajeevbhat@usm.my Tel: +60-4653-5212 (off)/

Dr. Rajeev Bhat is presently working as Associate Professor of Food Technology at Univeristi Sains Malaysia. Attaining self-sufficiency, ensuring food sustainability and application of novel techniques for preservation of food have been the major theme of his research since past fifteen years. Dr. Bhat has authored more than 150 research publications (in ISI journal and as book chapters) and has four books to his credit. He is a recipient of several prestigious international awards, such as: ‘Excellence Recognition Award’ (Association of Agricultural Technology in Southeast Asia), ‘Prosper.Net-Scopus Award in Sustainable Development’ (UNU), ‘IUFoST/Fi Young Scientist Excellence Award’ (International Union of Food Science and Technology, Canada), Global Achievers Award (conferred by IIOH, India), etc. As an active researcher he has worked under various capacities in India, South Korea, Malaysia and Germany.

July 1-3, 2015

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Invited Speakers "Can feedstuff processing improve digestibility of amino acids for nonruminants?"

Professor Dr. Julian WISEMAN Division of Animal Science, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire United Kingdom Phone: 0115 951 6054 Fax: 0115 951 6099 Email: julian.wiseman@nottingham.ac.uk

Prof. Dr. Julian Wiseman is a head of the Division and Senior Editor of the Journal of Agricultural Science, Cambridge. He has published 81 refereed papers and 37 review articles and has written three books. Julian's research interests fall into two main areas: non-ruminant animal nutrition and product quality. Current research areas include: nutritional value of co-products from bioethanol production, homegrown legumes in diets for non-ruminants; digestibility, performance and carcass quality, evaluating exogenous enzymes in pig nutrition and muscle growth in broilers.

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2 International Symposium on Agricultural Technology (ISAT2015)

"Opportunity of AEC meat production for Halal food market"

Professor Dr. Abdul Salam BABJI School of Chemical Science and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM) Selangor, Malaysia Phone: +603-89215988 Fax: +603-89213232j Email: daging@ukm.my

Prof. Dr. Abdul Salam Babji is currently working as a professor in National University of Malaysia; he is a senior ex-pert with more than 30 years of experience in the field of Meat Science and Meat Technology. One of his major areas of R&D is focused on fish, meat and poultry product development with emphasis on healthy meat products. He has been actively seeking ways into replacing synthetic food additives in meat products with phytochemical extracts of herbs and spices. These extracts are tested for their effectiveness as antioxidant and antimicrobial material, also as a tool to extend the quality and shelf life of meat products.

July 1-3, 2015

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"Applications of molecular diagnostic methods in the management of fungicide resistance"

Prof.Dr. Hideo ISHII School of Agricultural Regional Vitalization Kibi International University, Japan TEL: +81-799-42-4722 FAX: +81-799-42-4701 E-mail: h-ishii@kiui.ac.jp or hi481204@yahoo.co.jp

Honorary Researcher at National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan. His major research fields include Fungicide resistance of pathogens, Pathological specialization of fungi, Disease resistance in plants, Induction and mechanism of systemic acquired resistance in plants, Disease control by natural products.

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"Rapid generation technology for food and forage legumes - harnessing in vitro technologies for accelerating genetic gain"

Assoc.Prof.Dr. Janine CROSER Centre for Plant Genetics and Breeding The University of Western Australia , Australia Phone +61 8 6488 7951 Fax +61 8 6488 1140 Email janine.croser@uwa.edu.au

Dr Janine Croser is a Project leader within the Centre for Plant Genetics and Breeding (PGB), School of Plant Biology at The University of Western Australia. Her main research interest is innovative plant breeding technologies to accelerate genetic gain, working with pasture and grain legumes, oilseeds and specialty crops. As a chief investigator, her current projects include development of accelerated single seed descent technology for grain legumes and mining of wild Cicer relatives for identification of valuable traits for chickpea improvement. Dr Croser began her career in Cell Biology at The Victorian Department of Agriculture, undertaking research for Monsanto and VanderHave Research Netherlands, before completing her PhD with The University of Melbourne. She undertook postdoctoral training at the University of Saskatchewan before joining The University of Western Australia in 2002. Her group currently consists of two postdoctoral researchers and four technical staff.

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"Biogas reduces the carbon footprint of cassava starch: A comparative assessment with fuel oil"

Dr.Thierry TRAN Joint Research Unit QUALISUD, CIRAD, France, and Cassava and Starch Technology Research Unit (CSTRU) Faculty of Agro-Industry, Kasetsart University, Thailand Tel: +66-86 822 0362 fax: +66-2 940 5634 Email:Thierry.tran@cirad.fr

Dr. Thierry Tran is a researcher at CIRAD, the French International Research Centre for Agricultural Development, Montpellier, France. His work focuses on food processing & environmental footprints, using mainly Life Cycle Assessment (LCA), as part of the activities of the QUALISUD research unit. He is currently based at the Cassava and Starch Technology Research Unit (CSTRU), for a collaboration between CIRAD and Kasetsart University, Bangkok, Thailand. He is also involved with development projects for increased processing of roots and tubers, such as cassava, in developing countries in Africa and South America, as well as Southeast Asia.

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

ISAT2015 Program at A-One the Royal Cruise Pattaya Hotel, Chonburi, Thailand

Day 1: Wednesday, 1 July 2015 08:00-09:00

Registration and Reception Venue: In front of Suphannahong Room, 3rd Floor, Royal Wing

Opening ceremony

09:00-09:30

Venue: Suphannahong Room

09:30-10:15

Keynote address I : Dr. Hiroyuki KONUMA

10:15-10:30

Group Photo and Coffee break

10:30-11:00

Keynote address II : Prof. Metha WANAPAT

11:00-11:30

Keynote address III : Assoc.Prof. Rajeev BHAT

11:30-12:00 12:00-13:00

Poster viewing (odd number) Venue: In front of Panerai 1 Room,3rd Floor, Royal Wing Buffet Lunch Venue: Private Room, 2nd Floor, Royal Wing

Oral Presentation I Session 1: Plant and Soil for Sustainability Venue: Suphannahong Room

Session 2: Animal Production and Fisheries for Sustainability Venue: Panerai 1 Room

13:00-14:15

O-1 to O-5

13:00-14:00

O-15-O-18

14:30-15:15

O-6 to O-9

14:00-15:00

O-19-O-22

15:15-15:30 Session 1: Plant and Soil for Sustainability Venue: Suphannahong Room

Coffee break Session 3: Process and Environmental Technologies for Sustainability Venue: Panerai 1 Room

15:30-16:45

16:45-17:30 18:00-20:00

July 1-3, 2015

O-10 to O-14

O-23 to O-27

Poster Presentation (Even Number) Venue: In front of Panerai 1 Room,3rd Floor, Royal Wing Welcome Party (Buffet) Venue: Suphannahong Room

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2 International Symposium on Agricultural Technology (ISAT2015)

Day 2 : Thursday, 2 July 2015 Invited Session Venue: Suphannahong Room 09:00-09:30

Invited speaker I : Prof. Julian WISEMAN

09:30-10:00

Invited speaker II : Assoc.Prof. Janine CROSER

10:00-10:15

Coffee break

10:15-10:45

Invited speaker III : Prof. Abdul Salam BABJI

10:45-11:15

Invited speaker IV : Prof. Hideo ISHII

Oral Presentation II Session 4: Crop Protection for Sustainability Venue: Suphannahong Room 11:15-12:00

O-28 to O-30

12:00-13:00

Buffet Lunch Venue: Private Room, 2nd Floor, Royal Wing Session 4: Crop Protection for Sustainability Venue: Suphannahong Room

13:00-14:45

O-31 to O-37

14:45-15:00

Coffee break Session 4: Crop Protection for Sustainability Venue: Suphannahong Room

15:00-16:15

O-38 to O-42

Closing Ceremony 16:15-16:45

Invited speaker V : Dr. Thierry TRAN

16:45-17:15

ISAT 2015 Awards for Oral Presentation and Poster Presentation Closing speech

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Day 3 Friday, 3 July 2015 "Excursion" at Suphattra Land, Rayong Province 09.00-10.00 10.00-12.00

Journey to Suphattra Land, fruit garden, Klaeng, Rayong Province (Pick-up point at A-One the Royal Cruise Pattaya Hotel) Visit fruit garden and taste the Exotic Fruits: rambutan, durian, mangosteen, lansat, dragon fruit, jackfruit, mango, coconutâ&#x20AC;Śetc.

12.00-13.00

Lunch

13.00-14.00

Visit grape garden, orchid garden, salad hydroponics and honey production

14.00-15.00

Back to A-One the Royal Cruise Pattaya Hotel

15.00-16.00

Journey to Bangkok (Drop point at Suvarnabhumi Airport)

July 1-3, 2015

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Scientific Program

2 International Symposium on Agricultural Technology (ISAT2015)

Scientific Program

Wednesday, 1 July 2015 Plenary Session Venue: Suphannahong Room 8.00-9.00 Registration and Reception Opening ceremony

9.00-9.30

9.30-10.15

10.15-10.30

10.30-11.00

11.00-11.30

 VDO Presentation: Faculty of Agricultural Technology, KMITL  Report address Assoc. Prof. Sakchai CHOOCHOTE Dean, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang  Welcoming and Opening Speech Prof. Dr. Suchatvee SUWANSAWAT President, King Mongkut's Institute of Technology Ladkrabang Keynote address Status of Global Agriculture and Food Security Situation, and the Future Trend Dr. Hiroyuki KONUMA Assistant Director –General and FAO Regional Representative for Asia and the Pacific Coffee break Keynote address Optimizing the Use of Feed Resources for Healthy Rumen, Increased Production and Mitigation Methane Prof. Dr. Metha WANAPAT Director of the Tropical Feed Resources Research and Development Center (TROFREC), Khon Kaen University, Thailand Keynote address Food Sustainability-Emerging Challenges in the ASEAN Region (Reality Versus the Myth) Assoc. Prof. Rajeev BHAT Food Technology Division, School of Industrial Technology, Universiti Sains Malysia (USM), Malaysia

11.30-12.00

POSTER VIEWING (Odd Number)

12.00-13.00

Lunch

July 1-3, 2015

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Scientific Program

Wednesday, 1 July 2015 Session 1: Plant and Soil for Sustainability Venue: Suphannahong Room Chairperson: Prof. Dr. Singam Laxmana SWAMY Secretary: Asst. Prof. Dr. Teerawat SARUTAYOPHAT Adapting the CSM-Canegro-Sugarcane Model to Simulate 13.00-13.15 O-1 Napier Grass in Chiang Mai Province, Thailand Tupthai NORSUWAN, Attachai JINTRAWET and Carsten MARONH

13.15-13.30 O-2

Effect of Azolla (Azolla spp.) on Growth and Yield of Pathumthani 1 Rice (Oryza sativa L.)

13.30-13.45 O-3

Experiences with Direct Seeded Rice in Jammu and Kashmir, India

Nattawan BUSSABA, Phanupong PHONCHAROEN and Teerawat SARUTAYOPHAT

Vijay BHARTI, Ashok KUMAR RAINA and Anuradha SAHA

13.45-14.00 O-4

Allelopathic Effects of Pisonia grandis R.Br. on the Germination and Growth of Echinochloa crus-galli (L.) Beauv. Sudteerak SAIPLUEMCHIT and Chamroon LAOSINWATTANA

14.00-14.15 O-5

How Far Teak Plantations Can Contribute for Climate Change Mitigation and Landscape Restoration in Tropics: A Case Study from Central India Singam Laxmana SWAMY and Alka MISHRA

Chairperson: Assoc. Prof. Dr. Janine CROSER Secretary: Asst. Prof. Dr. Kanjana SAETIEW Antioxidant Properties of Flower Extract of Etlingera 14.15-14.30 O-6 elatior (Jack) R.M. Smith Kanokporn CHANGSAWAKE, Komkhae PILASOMBUT, Chamroon LAOSINWATTANA and Montinee TERARAK

14.30-14.45 O-7

Effect of Storage Conditions on the Vase Life of Homalomena Philodendron and Monstera Nipaporn YONSAWAD and Montinee TEERARAK

Micro-propagation of Dalbergia cochinchinesis Pierre 14.45-15.00 O-8

15.00-15.15 O-9

Wannasiri WANNARAT, Panida WONGWEAN, Sirinaree SUPANSOMPORN, Warinee KITPRECHAWANICH and Yupa PANKAEW

Standardization of Drip Irrigation and Fertigation for Improving Physiology, Yield and Quality Parameters of Mango var. Alphonso under Ultra-High Density Planting K. PRAKASH, R.M. VIJAYAKUMAR and S.D. SUNDHAR SINGH

15.15-15.30 Coffee break

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Scientific Program

Session 1: Plant and Soil for Sustainability Venue: Suphannahong Room Chairperson: Assoc. Prof. Dr. Somsak MANEEPONG Secretary: Asst. Prof. Dr. Nukoon TAWINTEUNG Study of Using Coop Fertilizer Ameliorant on Greenhouse Gas Emission in Some Rubber Estates 15.30-15.45 O-10 Agroecosystems in Peatland Hariyadi JAMIN PRIYO MINARJO, Dedi NURSYAMSI and Adi PRADIPTA

Plant Nutrient Management Strategies Enhanced Growth, Yield Traits and Mitigated Leaf Reddening in 15.45-16.00 O-11 Cotton (Gossypium hirsutum L.) Allahwadhayo GANDAHI, Khalillulah PANHWAR and Rabail GANDAHI

Utilization of Calcium Silicate Application on Pepper 16.00-16.15 O-12 Seedling Production

Eakkarin SUKKAEW, Suphachai AMKHA, Thongchai MALA and Pornpairin RUNGCHAROENTHONG

Behavior of Nutrient Uptake by Pummelo Growing on 16.15-16.30 O-13 Salt Marsh Soil Hien HUU NGUYEN, Somsak MANEEPONG and Potjamarn SURANINPONG

Eco-Friendly Utilization of TDE and Fertilizers on Soil 16.30-16.45 O-14 Properties, Yield and Quality of Seed Cane J. REVATHI and M. BASKAR

16.45-17.30 POSTER VIEWING (Even Number)

18.00-20.00

July 1-3, 2015

Welcome Party (Buffet) Suphannahong Room

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Scientific Program

Session 2: Animal Production and Fisheries for Sustainability Venue: Panerai 1 Room Chairperson: Dr. Isao TSUTSUI Secretary: Asst. Prof. Dr. Monthon GANMANEE A Co-Culture System of Giant Tiger Prawn and Unexploited Seaweed and Snail 13.00-13.15 O-15 Isao TSUTSUI, Dusit AUE-UMNEOY, Jaruwan SONGPHAT

KAEW, Piyarat PINPHOO, Chonlada MEEANAN, Sirimas KLOMKLING, Halethichanok SUKCHAI, Monthon GANMANEE and Kaoru HAMANO

Investigating the Safety of Potential Probiotic 13.15-13.30 O-16 Candidates Isolated from the GI Tract of Tilapia in vitro Rungtawan YOMLA, Daniel MERRIFIELD and Simon DAVIES

Studied on Using of Robo-Logger Technique Measuring 13.30-13.45 O-17 In Situ Inside Shell Temperature of Rocky Shore Limpet Monthon GANMANEE, Chayanid MEEPOKA, Pokamon KOCHARIN and Gray A. WILLIAMS

Impacts of the PTT GC Oil Spill on Intertidal Rocky 13.45-14.00 O-18 Shore Macrobenthos in Ao Phrao, Samed Island Jindarha PREMPRAMOTE, Chayanid MEEPOKA, Sujitra SAMAKRAMAN and Monthon GANMANEE

Chairperson: Prof. Dr. Julian WISEMAN Secretary: Assoc. Prof. Dr. Ronachai SITTHIGRIPONG A Study on Some Production Traits and Egg Quality 14.00-14.15 O-19 Characteristics of Lutein Eggs Kanda LOKAEWMANEE and Supat DUANGDEE

14.15-14.30 O-20

Effect of Leucaena Silage Levels on Rumen Fermentation and Nutrients Digestibility in Dairy Steers Giang NGUYEN THIEN TRUONG and Metha WANAPAT

14.30-14.45 O-21

Effect of Dried Leucaena Leaf and Leucaena Silage Supplementation on Rumen Ecology, Rumen Fermentation in Swamp Buffalo Kampanat PHESATCHA and Metha WANAPAT

14.45-15.00 O-22

Supplementation of Urea on Nitrogen Balance and Microbial Protein Synthesis in Swamp Buffaloes Fed on Cassava Hay and Rice Straw Thiwakorn AMPAPON, Metha WANAPAT and Kampanat PHESATCHA

15.00-15.30

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Coffee break

July 1-3, 2015

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Scientific Program

Session 3: Process and Environmental Technologies for Sustainability Venue: Panerai 1 Room Chairperson: Assoc. Prof. Rajeev BHAT Secretary: Asst. Prof. Dr. Lampan KHURNPOON Effects of Drying Process on Characteristics of Gac 15.30-15.45 O-23 Fruit Aril Powder

Warangkana ANGKANANON, Mudtorlep NISOA, Phanphen ATTANARSAKIT, and Visaka ANANTAWAT

Modeling the Microwave Heat Distribution of 15.45-16.00 O-24 Banana at Different Ripening Stage W. THUTO and K. BANJONG

Preparation, Optimization and Characterization of 16.00-16.15 O-25 Carboxymethyl Cellulose from Rice Straw Using Microwave Heating Noppadol PANCHAN and Chalida NIAMNUY

Optimization of Process Parameters on the Production 16.15-16.30 O-26 of Bacterial Cellulose from Rice Rinsing Waste Water (nata-de-leri) by Acetobacter xylinum Alwani HAMAD, Giswantara and Endar PUSPAWININGTYAS

Orthogonal Functions for Mathematical Modeling in 16.30-16.45 O-27 Problems on Agriculture Technology Mohsen RAZZAGHI

16.45-17.30 POSTER VIEWING (Even Number)

18.00-20.00

July 1-3, 2015

Welcome Party (Buffet) Suphannahong Room

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Thursday, 2 July 2015 Invited Session Venue: Suphannahong Room

9.00-9.30

Invited speaker Can Feedstuff Processing Improve Digestibility of Amino Acids for Non-ruminants? Prof. Dr. Julian WISEMAN

Division of Animal Science, School of Biosciences, Nottingham University, United Kingdom

9.30-10.00

Invited speaker Rapid Generation Technology for Food and Forage LegumesHarnessing in vitro Technologies for Accelerating Genetic Gain Assoc. Professor Dr. Janine CROSER

Centre for Plant Genetics and Breeding, The University of Western Australia (M080), Australia 10.00-10.15

Coffee break

10.15-10.45

Invited speaker Opportunity of AEC Meat Production for Halal food Market Prof. Dr. Abdul Salam BABJI

School of Chemical Science and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia

10.45-11.15

Invited speaker Applications of Molecular Diagnostic Methods in the Management of Fungicide Resistance Prof. Dr. Hideo ISHII

School of Agricultural Regional Vitalization Kibi International University, Japan

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Session 4: Crop Protection for Sustainability Venue: Suphannahong Room Chairperson: Prof. Dr. Hideo ISHII Secretary: Dr. Nonglak PARINTHAWONG Sensitivity Detection of Phytophthora spp. Causing Para11.15-11.30 O-28 Rubber Leaf Fall Disease to Some Systemic Fungicides Pornprapa KONGTRAGOUL and Panot VIRIYAEKKUL

Assessment of the Antagonistic Activity of Trichoderma spp. 11.30-11.45 O-29 from Five Different Habitats on Plant Pathogenic Fungi

Suriyasit SOMNUEK, Pornprapa KONGTRAGOUL and Tanimnun JAENAKSORN

Colonization of Plant Root and Punctured Surface Tissue by 11.45-12.00 O-30 Non-Pathogenic and Pathogenic Fusarium oxysporum Titi THONGKAMNGAM and Tanimnun JAENAKSORN

12.00-13.00

July 1-3, 2015

Lunch

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Session 4: Crop Protection for Sustainability Venue: Suphannahong Room Chairperson: Prof. Dr. C. P. KHARE Secretary: Dr. Nonglak PARINTHAWONG, Dr. Pornprapa KONGTRAGOUL Integrated Management of Yellow Vein Mosaic Disease 13.00-13.15 O-31 in Okra

C. P. KHARE, A. KOTESTHANE, D. SHARMA, A. DIXIT, and J. SINGH

In Vitro Production of Cell Wall Degrading Enzymes by Pythium Species Isolated from Asymptomatic and 13.15-13.30 O-32 Symptomatic Lettuce Root Chulalak TALUBNAK, Nonglak PARINTHAWONG and Tanimnun JAENAKSORN

Gene Expression Analysis in Lettuce (Lactuca sativa L.) 13.30-13.45 O-33 Treated with Trichoderma spp. Malatee PRADUBYAT, Nonglak PARINTHAWONG and Tanimnun JAENAKSORN

Evaluation of Photosynthesizing Bacteria for the 13.45-14.00 O-34 Growth of Rice var. RD41 Rungrat VAREEKET and Kasem SOYTONG Assessment of Viability and Efficacy of Fusarium oxysporum (F221-B) as BCA and PGPF During Long 14.00-14.15 O-35 Term Preservation Titi THONGKAMNGAM and Tanimnun JAENAKSORN

Comparison of Trichoderma Population in the ReCirculating Nutrient Solution With and Without 14.15-14.30 O-36 Supporting Material Kanet JAIKENGKAJ, Tanimnun JAENAKSORN and Prommart KOOHAKAN

Morphological Identification of Trichoderma species 14.30-14.45 O-37 from Different Habitats

Suriyasit SOMNUEK, Pornprapa KONGTRAGOUL and Tanimnun JAENAKSORN

14.45-15.00

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Coffee break

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Session 4: Crop Protection for Sustainability Venue: Suphannahong Room Chairperson: Prof. Dr. P.K. TIWARI Secretary: Asst. Prof. Dr. Ammorn INSUNG Resistant in Rice Cultivars against Sheath Blight 15.00-15.15 O-38 Disease under Artificial Inoculated Conditions P.K. TIWARI, C.P. KHARE and A.S. KOTASTHANE

15.15-15.30

Influence of Weather Parameters on the Incidence of Okra Yellow Vein Mosaic Virus Disease and Whitefly O-39 (Bemisia tabaci) Population D.R. BHENDARKAR, C.P. KHARE and S.L. SWAMY

15.30-15.45

Rhodospirillum spp. from Wastewater O-40 Rungrat VAREEKET and Kasem SOYTONG

15.45-16.00

A Crossbred High Yielding and Insect Resistant Cotton O-41 Variety Having Wider Climatic Adaptability

16.00-16.15

The Impact of Climate Variability on Agricultural Pest O-42 Ninio A. RELOX and Sharon Juliet M. ARRUEJO

Syed BILAL HUSSAIN, Zulfiqar ALI and Hasnain NAWAZ KHAN

Closing Ceremonies 16.15-16.45

 Invited Article Biogas Reduces the Carbon Footprint of Cassava Starch: A Comparative Assessment with Fuel Oil Dr. Thierry TRAN Researcher, CIRAD, France

16.45-17.15

 ISAT 2015 Awards for Oral Presentation  ISAT 2015 Awards for Poster Presentation  Closing Speech

July 1-3, 2015

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Friday, 3 July 2015

Excursion to Suphattra Land, fruit garden, Klaeng, Rayong Province

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Poster Session: Global Agriculture Trends for Sustainability

P-1

Effect of Naphthalene Acetic Acid and Thidiazuron on Phytotoxicity of Phenanthrene and Fluorene to Waxy Corn

P-2

Effect of Fertilizers on Physiological Traits Relate to Yield of Napier Pak Chong 1 Grass

P-3 P-4

Waraporn CHOUYCHAI, Jaturaporn DONRODPRI and Khanitta SOMTRATOON

Nittaya PHAKAMAS, Anupong MAKLAI, Tochchakorn PERMHIRUN, Kittiya SERTSUNGNONE and Nawarat JAMPATHONG

Effect of Nanoparticles on the Relationship between Crop Growth Rate and Yield of Chainat 1 Rice (Oryza sativa L.) Sutichai SAMART, Nittaya PHAKAMAS and Sutee CHUTIPAIJIT Antioxidant Determination of Nang Dam Upland Rice Bran Oil by DPPH Assay Vanapron SAE-ANG, Chitti TAWAI and Duangkamol RUEN-NGAM

P-5

Effect of Different Media and Concentrations of Growth Regulator on Callus Induction and Growing Suspension Cell Culture of SanPah-Tawng 1(Oryza sativa L.) Ranyikar PORAHA, Anurug POEAIM, Saengthong PONGJAROENKIT and Pradit PONGTHONGKAM

Elite Thein Corn Inbred Lines Utilized to be Synthetic Variety

P-6

Kitti BOONLERTNIRUN, Suchada BOONLERTNIRUN and Choosak JOMPUK

Hypoxic Responses of 6 Commercial Waxy Corn Varieties

P-7 P-8

Suchada BOONLERTNIRUN and Kitti BOONLERTNIRUN, and Raweewun SUVANNASARA

Development of Chitosan-Based Silver Nanoparticles Coating and Study of Its Effect on Litchi Stored at Ambient Temperature Warin PIMPA and Chakkrit PIMPA

P-9

P-10

Green Synthesis of Silver Nanoparticles Loaded onto Activated Carbon Using Banana Peel Extract for Environment Applications Warin PIMPA and Chakkrit PIMPA Bioactive Compounds and Antioxidant Activities in Flesh, Leaves and Endocarp of Carissa carandas Compared to Grape Seed Extract Products Surasak SAJJABUT, Wachiraporn PEWLONG, Sirilak CHOOKAEW, Jaruratana EAMSIRI and Panchalee PRAKHONGSIL

P-11

Gamma Radiation Effects on Anthocyanins, Vitamin C Content and Antioxidant Activity of Carissa carandas

P-12

Influence of Electron Beam Irradiation on Hygienic Quality and Antioxidant Activities of Ground Sea Holly (Acanthus ebracteatus)

Wachiraporn PEWLONG, Surasak SAJJABUT, Jaruratana EAMSIRI, Sirilak CHOOKAEW and Panchalee PRAKHONGSIL

Jaruratana EAMSIRI, Surasak SAJJABUT, Wachiraporn PEWLONG and Sirilak CHOOKAEW

July 1-3, 2015

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P-14

Effect of Alginate Edible Coating on Quality of Fresh-cut ‘Kimju’ Guava A. ONDEE and S. MANURAKCHINAKORN Evaluation of Antioxidant Activities of Essential Oils from Peppermint and Ginger

P-15

Improved Efficiency of Anthocyanin Extraction from Roselle Using Acidified Solvent

P-13

Natthakiti PHURUEN, Chamroon LAOSINWATTANA and Montinee TEERARAK

F. NUUDOM and S. MANURAKCHINAKORN

P-16

Antioxidant Activity and Total Phenolic Contents of Tagetes erecta L. Leaves Extracts Pattharin WICHITTRAKARN, Montinee TEERARAK and Chamroon LAOSINWATTANA

P-18

Allelopathic Effect of Fresh and Dried Leaves Aqueous Extracts of Marachra capitata L. and the Action of Allelochemicals in Different Soil Types Phawinee KAMSAN, Pattharin WICHITTRAKARN, Montinee TEERARAK, and Chamroon LAOSINWATTANA Effects of Natural Herbicide from Piper betle Linn on Seed Germination, Imbibition and α- Amylase Activity of Amaranthus gracilis Desf.

P-19

Effect of Different Temperatures on Carotenoid Content and Antioxidant Activity in ‘Khak Dam’ Papaya

P-17

Pariyaporn NETSAWANG, Pattharin WICHITTRAKARN, Montinee TEERARAK, and Chamroon LAOSINWATTANA

Kanthee SIRIVEJABANDHU and Lampan KHURNPOON

P-20

Nitric Oxide Improves Cowpea (Vigna unguiculata [L.] Walp.) Growth under Lead Stress Omid SADEGHIPOUR

P-21

Nutrient Dynamics in an Aquaponic System Somsak MANEEPONG

The Effects of Calcium Silicate on Density of Trichomes in Field Corn

P-22 P-23

Pongsakorn NITMEE, Pornpairin RUNGCHAROENTHONG, Suphachai AMKHA and Thongchai MALA

Utilization of Zeolite and Isolite as Soil Conditioner to Improve Sandy Soil Nukoon TAWINTEUNG

Dendoremediation Efficiency of Forest Hardwoods to Remove Cadmium Toxicity from Contaminated Soil

P-24

P-25

Syed AMIR MANZOOR, Din MUHAMMAD ZAHID KHAN, Muhammad ZUBAIR, Wasif NOUMAN, Sarwat NAZ MIRZA, Syed BILAL HUSSAIN, Feehan HASSAN and Muhammad IMRAN UMAR

Leaf Flushing Inhibition by Application of High Phosphorus and Potassium Fertilizers for Off-season Mango Production Kannikar KAEWSONGSANG, Apisak BAOLEE and Ravie SETHPAKDEE

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P-26

Sand-Size Distributions of Soils on the So-Called Aeolian Sand Splay Landform in the Lower Mun-Chi Basin, Northeast Thailand Pornthiwa KANYAWONGHA and Anongnat SRIPRACHOTE

P-27

Parent Material Affecting Phosphorus Distributions in Acid Sulfate Soils of Thailand Pornthiwa KANYAWONGHA, Nucharee BOONPLANG and Anongnat SRIPRACHOTE

P-28

Screening of Ametryn Resistant Bacteria from Sugarcane Cultivation Soils Pattrarat TEAMKAO, Nipaporn ROENGANAN and Siratee PONGPOUN

P-29

Effect of Organic Fertilizers on Soil Phosphorus Sorption Capacity Sukunya YAMPRACHA and Parichart WATHAKIATTIKUL

P-30

A Relationship between Loss on Ignition and Carbon Concentration Determined by Wet Digestion to Assess C/N Ratio of Plant Materials and Organic Fertilizers Nukoon TAWINTEUNG

P-31

Relationship between Total Calcium and Pectin in Asparagus (Asparagus officinalis L.) Cell Wall Nutcharee BOONPLANG

P-32

Antibacterial Activity of Roselle (Hibicus sabdariffa Linn.) Flower Extract Napaporn KONGKARN, Pussadee TANGWATCHARIN, Montinee TEERARAK, Kanokporn CHANGSAWAKE and Komkhae PILASOMBUT

P-33

Isolation of ACC-Deaminase-Producing Endophytic Bacteria from Rice (Oryza sativa) Chokchai Kittiwongwattana

P-34

In vitro Antagonistism of Talaromyces flavus and Neosartorya pseudoficheri Against Anthracnose Disease on Coffee Mayamor SOYTONG and Supattra POEAIM

P-35

Identification of Blast Resistance Gene in Yang Mawng Variety of Thai Indigenous Rice Siriporn PRAMRIT and Nonglak PARINTHAWONG

P-36

Acaricidal Activity of Paracress (Acmella oleracea (L.) R.K. Jansen) Extract on the Mushroom Mite (Dolichocybe indica Mahunka) Jarongsak PUMNUAN, Jakkrapong AREEWONG, Pornprapa KONGTRAGOUL and Ammorn INSUNG

P-37

A Degree-Day Simulation Model for the Population Dynamics of the Brown Planthopper, Nilaparvata lugens Stal. (Homoptera: Delphacidae) Wirote KHLIBSUWAN, Yupa HANBOONSONG and Krirk PANNANGPETCH

P-38

Preliminary Study on Used Microalga (Nostoc commune) as a Protein Supplement in Dry-Wet Mixtures Feed for Juveniles Snakehead (Channa striata) Suneerat RUANGSOMBOON

July 1-3, 2015

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P-39

P-40 P-41

Protein and Some Other Constituents of Sucker Catfishâ&#x20AC;&#x2122;s Fish Meal Kalkullanutch PATRARASRIPONG and Kanok LERTPANICH

Protein Isolation from Bigeye Snapper (Priacanthus tayenus) Head By-Product Using pH-Shift Method Worawan PANPIPAT and Manat CHAIJAN

P-42

Myoglobin Redox Instability of Protein Isolate from Bigeye Snapper (Priacanthus tayenus) Head By-product Worawan PANPIPAT and Manat CHAIJAN

P-43

Utilization of Fish Residue from Fish Sauce Fermentation for High Calcium Sweet Dipping Sauce (Nam Pla Wan) Production Manat CHAIJAN, Worawan PANPIPAT and Waraporn TUMTONG

P-44

Genetic Diversity of Seagrasses across the Eastcoast of Thailand Based on Sequence-Related Amplified Polymorphism (SRAP) Technique Pattama SRINAMNGOEN and Kanok-on DUANGPAKDEE

P-45

Genetic Variation of Halodule pinifolia Collected from Rayong Province Using SRAP Marker Siraphop ADICHANUND and Pattama SRINAMNGOEN

P-46

Aquatic Polyculture Farm in Bangsaothong District, Samutprakarn Province, Thailand Nipon JITTAMNAN1 and Panneepa SIVAPIRUNTHEP

P-47

Microbiological Safety of Ready-to-Eat Semi-Dried Nham, an Innovation of Thai Fermented Meat Product Thanapa CHETAWAN, Komkhae PILASOMBUT and Supaluk SORAPUKDEE

Carcass Traits Comparison between Duroc and Commercial Crossbred Pigs

P-48

Numfon TAJASRI, Chanporn CHAOSAP, Ronachai SITTHIGRIPONG and Rutcharin LIMSUPAVANICH

P-49

Optimization of Culture Conditions of Bacteria Isolated from Buffalo Rumen for Cellulase and Xylanase Production

P-50

Consumption of Energy, Water and Consumables in Pon-YangKham Beef Cutting Process

P-51

Gel Properties of Pork Ball Containing Mixed Precooked Carotenoid-rich Vegetables

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Kanya JIRAJAROENRAT, Jutatip CHALERMWANICHWONG, Tipaporn NGAMSANGA and Kanokrat SRIKIJKASEMWAT

Chalermsak SAKDAPECHSIRI, Thierry TRAN, Matana OSOTHONGS and Kanya JIRAJAROENRAT

Manat CHAIJAN, Worawan PANPIPAT, Noppawan LAOPHROM and Saowanee TRUKTRONG

July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Scientific Program

P-52

Changes in Physicochemical, Microbiological and Sensorial Quality of Jerky Processed From Spent Laying Hen Meat during Storage

P-53

Screening of Lactic Acid Bacteria from Traditional Fermented Meat Products

P-54

Screening and Identification of Lactic Acid Bacteria with Antimicrobial Activity to Mastitis Pathogen in Dairy cows; Staphylococcus aureus

P-55

Comparison of Stomoxys calcitrans (Diptera: Muscidae) Attacked and Behavioral Responses on Genetic Blood Differences of HolsteinFriesian Dairy Cattle in Thailand

Chanpen UESAKULRUNGRUENG, Supaluk SORAPUKDEE and Komkhae PILASOMBUT

Jirarot NITHISANTAWAKHUPT, Pussadee TANGWATCHARIN and Kan SUKSUPATH

Khakhanang RATANANIKOM, NantiyaSUWANPANYA, Yupaporn KHONNALAO and Sarayut SRIROD

Ubon TANGKAWANIT, Varangrat SENASING, Wirote KHLIBSUWAN, and Tassanee JAMJANYA

P-56

Opinion Towards Agricultural Further Study of Student in Nongchok District, Bangkok Metropolis Tippawan LIMUNGGURA and Panya MANKEB

P-57

Using Augmented Reality (AR) Technology for the Promotion of Agricultural Products: A Case Study of Bang Krathum Processed Banana Product Nutthakorn SONGKRAM

P-58

Factors Affecting Consumer Purchasing Behavior of Toxic-Free Vegetables in Muang District, Samutprakarn Province

P-59

Financial Cost and Benefit Analysis of Commercial Orchid Farming in Krathumban District, Samutsakorn Province, Thailand

P-60

Enhance Environment of a Historic Site, Ancient Benhama Maharat Building, For Future Public Use

P-61

Biodiesel Synthesis from Used Frying Oil with the Modified Catalyst from Waste Anadara granosa Shells

Panya MANKEB, Pratompong MATONG, Suneeporn SUWANMANEEPONG and Prapaporn CHULILUNG

Panya MANKEB, Chalida LERTKRASEMPON, Tippawan LIMUNGGURA and Prapaporn CHULILUNG

Warong NAIVINIT, Parkpoom SUEBNUKARN, Rukkeit SANPRASERT and Taweesak WIYACHAI

Sasiwimol WOOTTHIKANOKKHAN, Buppha THAMNIYOM and Supamas CHUANOI

Scanning Electron Microscope of Alkaline Treated Spent Coffee Ground

P-62 P-63

Chayaporn WONGSIRIDETCHAI, Watcharaphan CHIANGKHAM, Narissara KHLAIHIRAN and Sudathip CHANTORN

Production of α-Amylase by Newly Isolated Thermotolerant Bacillus aerius through Solid State Fermentation by Using Some Agricultural Wastes Veysi OKUMUŞ, Sadin ÖZDEMİR and Abdurrahman DÜNDAR

July 1-3, 2015

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Keynote and Invited Abstracts

July 1-3, 2015

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Keynote and Invited Abstracts

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Keynote and Invited Abstracts

Optimizing the Use of Feed Resources for Healthy Rumen, Increased Production and Mitigating Methane in Ruminants Professor Dr. Metha WANAPAT Director of the Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand Email: metha@kku.ac.th

ABSTRACT Ruminants are important livestock capable of utilizing fibrous feeds in the rumen through the fermentation of microorganisms, including bacteria, protozoa and fungi. Their fermentation end-products in the rumen are those of volatile fatty acids (VFAs), ammonia nitrogen (used for microbial protein synthesis), and methane production. Local feed resources availability in various seasons can contribute as essential sources of carbohydrate and protein and can significantly impact to the rumen fermentation and the subsequent productivity of the ruminant. Manipulation of rumen fermentation to maintain rumen ecology with normal pH, essential microbes and fermentation end products such as VFA and ammonia nitrogen concentration are essential. Furthermore, manipulation of the rumen using dietary approach to mitigate methane is promising. Development of the foodfeed-system (FFS) can increase food for human and feed for ruminant as well as enrich the nitrogen for the soil. Cassava root is one of the good examples, in the form of dry cassava chips, pellets, or by-product from starch factory as energy sources, and dried cassava leaves and/or cassava hay as protein sources, have been used successfully in ruminant rations. It was found that the uses of cassava could provide year-round feed, which resulted in a high yield and good quality milk, and contributed to a more lucrative dairy and beef cattle, buffalo enterprise, especially for smallholder ruminant farming systems. Apart from producing rumen volatile fatty acid and microbial protein, greenhouse gas such as methane is also produced in the rumen. There have been a number of methods used in reducing rumen methane. However, among many approaches, nutritional manipulation using feed formulation and feeding management, especially the use of plant extracts or plants containing secondary compounds (condensed tannins and saponins), banana flower powder (BAFLOP) rich in minerals, as well as plant oil have been reported with promising results. Such results have impacted on decreasing rumen protozoa, methanogens and methane mitigation. However, at the current stage, more research concerning this

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burning issue with the role of livestock on global warming warrants further research undertakings regarding economical feasibility and practicality especially on the wider scope of implementation. Keywords: Rumen manipulation, Methane, Plant secondary compounds, Feeding system

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Food Sustainability - Emerging Challenges in the ASEAN Region (The Reality versus the Myth) Associate Professor Dr. Rajeev BHAT Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Penang- 11800, Malaysia Email: rajeevbhat1304@gmail.com, rajeevbhat@usm.my

ABSTRACT On a global perspective, what does ‘Food sustainability’ technically mean? Is there any appropriate definition? Otherwise, how to segregate ‘Food Sustainability’ and ‘Sustainable Foods?’ Overall, the word ‘Sustainability’ literally means “the ability to satisfy the needs of our present generation by ensuring that the needs of the future generation remains uncompromised.” According to the United Nations, food sustainability aims “to ensure nutritional security without foregoing the long-term health of the ecosystem and the vital cultural scenario that provides food.’ However, on a broader perspective, food sustainability encompasses a wide array of multidisciplinary themes, which can have an extensive paradigm (developing and implementation of novel concepts, hypothesis, policies, theories and ideas, etc) relevant to the ‘socio-economic’ state-of-affairs up to the ‘agroecological-food sector’ scenario. The concept and theme of food sustainability covering an academic notion can differ from those of agrofoods based industries. On a broader sense, food sustainability can be linked with ensuring food security (quality and safety, overcoming hidden hunger, population explosion and poverty, food loss/wastage, food governance and food crisis, food trade, etc) as well as attaining a successful ‘sustainable food production.’ In the ASEAN context, several emerging challenges are faced concerning the production of sustainable foods or for achieving comprehensive food sustainability. The theoretical policies and framework put-forward for the developed countries might not be of total practical apprehension in the developing world, as in the case of ASEAN region. This requires to be amended based on the basic requirements of the region. Besides, the regional polices can widely differ from the proposed/practised international policies. It is worth to accentuate that some of the claims made in the developing world (as in case of ASEAN countries) might me more of a “myth” rather the ‘truth/reality.’ In the ASEAN region, individual governments have been proposing diversified trend marks relevant towards ensuring food security/food sustainability. However, we need to comprehend

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whether the entire proposal is a reality (practically applicable), and if yes, how many ASEAN countries are self-reliable and food secured? Further, when it comes to the societal needs, is healthy, nutritious and a wholesome food being consumed by the local populations? Is there any encouragement given to the local populations to promote and encourage consumption of local and a traditional healthy sustainable food? How about underutilized resources? What are the priorities set in this regard? On a common platform, is there any knowledge shared between the government, NGO’s, concerned industries and consumers on the importance of sustainable foods? If yes, how far is this successful? Any detailed documentation is being prepared in the ASEAN context? Further, when it comes to the agro-food industry sector, are the new proposed policies and themes in the ASEAN region, such as those concerning the effects of greenhouse gases, pollutants along the food chain, reusing/reprocessing of agro-food wastes, conservation of water, fuel and energy, bio-degradable packaging materials, impact of novel food processing technologies (e.g. nanotechnology), and other requirements are successfully implemented? Moreover, what are the roles that need to be played by the ‘urban’ versus ‘rural’ populations to ensure food sustainability in the region is a success? How to reconnect this diversified group of populations (intra- and inter-)? Further, when it comes to the farming community, is adequate care being taken to ensure that sharing of knowledge relevant to agro-food sustainability, new governmental policies, land use planning, impacts of weather changes and natural calamities, new ways of financial assistance (assuring economic self-sufficiency), active inclusion of farmers in the government’s decision making processes are crafted? Then how about achieving sustainability in the feed processing industry (for livestock)? Finally, can we come up with a common ‘vision’ and ‘mission’ (agenda and a frame work) for the ASEAN region relevant to attaining food sustainability? What should be our new approaches in the ASEAN region to benefit our community for sustainable development? All of the above facts comparing the emerging challenges, the ‘Reality versus the Myth’ in the ASEAN regional context of ‘Food Sustainability’ will be discussed/deliberated in this talk. Keywords: Sustainable Foods, Farming Community, Governmental Policies, Food Security, Consumer

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Keynote and Invited Abstracts

Can Feedstuff Processing Improve Digestibility of Amino Acids for Non-ruminants? Professor Dr. Julian WISEMAN Professor of Animal Production Division of Animal Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, UK

ABSTRACT Non-ruminant livestock diets in most regions of the world are based on cereals / cereal co-products (for example wheat distillers dark grains with solubles â&#x20AC;&#x201C; WDDGS - from the rapidly developing bioethanol industry) and plant proteins that are particularly important in view of the current ban on animal proteins within the European Union. Plant proteins invariably contain naturally-occurring anti-nutritive factors, principally trypsin inhibitors that are particularly important in soya beans but also occasionally peas. The inhibitors are heat labile and denatured by heat. There are a number of technologies available for processing plant proteins but a key message is that equipment operates under variable conditions of temperature, duration and moisture addition. Over-processing risks protein being denatured; for example a trypsin inhibitor activity of 1.5mg/g is associated with a reduction in amino acid digestibility. It is crucial that processing conditions are defined accurately rather than simply name of equipment. Extrusion is a common technology used in processing raw materials. Investigations into amino acid digestibility of soybeans following extrusion have produced data confirming that both under and over-cooking will give lower figures than when conditions are optimum. Levels of the trypsin inhibitor are lower in peas (a crop that is fairly common in many parts of the world) so the need for extrusion is not as important, in fact peas appear to be more sensitive to overcooking than soybeans so there is a greater risks of reducing amino acid digestibility. The main objective of cereal fermentation is to convert starch to bioethanol leaving WDDGS; this is moist and therefore has to be dried as quickly as possible. Unfortunately it appears that over-cooking reduces amino acid digestibility. The conclusion is that processing can indeed improve amino acid digestibility but this needs to be carefully controlled in order to optimise nutritional value of raw materials.

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Rapid Generation Technology for Food and Forage Legumes - Harnessing in vitro Technologies for Accelerating Genetic Gain Assistant Professor Dr. Janine CROSER Centre for Plant Genetics and Breeding, The University of Western Australia, Australia

ABSTRACT Rapid development of recombinant inbred line (RIL) populations for exploitation of powerful new molecular technologies is just one benefit of new tools to accelerate generation turnover in grain legumes. We have developed accelerated single seed descent (ASSD) technology to achieve a turnover of six to eight generations per year for the food legumes field pea (Pisum sativum L.), chickpea (Cicer arietinum L.), lentil (Lens culinaris Medik.) and narrow-leaf lupin (Lupinus angustifolius L.). We have adapted this technology to the forage legumes, including subclover and Medic species. The ASSD technique is robust, cost-effective and enables the development of RIL populations in less than 12 months. We are currently working to incorporate into the ASSD methodology targeted screening for tolerance to abiotic traits of interest (viz. boron toxicity in pea and lentil; low pH and aluminium toxicity in lupin and chilling tolerance in chickpea). It is envisaged combining ASSD with abiotic stress screening will provide efficient selection of adapted germplasm for rapid incorporation into breeding programs and thus permit a timely response to emerging challenges.

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Opportunities of AEC Meat Production for the Halal Food Market. BABJI, A.S.*, SAHILAH, A.M., NURâ&#x20AC;&#x2DC; ALIAH, D. and NURUL NADIA, M. School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi Selangor, Malaysia Tel: 603-89215988, Fax: 603-89213232 *Email: daging@ukm.edu.my

ABSTRACT Exploring and identifying the strengths of ASEAN in the production of Halal meat and processed value added meat will be the best approach towards a win-win venture between member countries. Looking at the current population growth of Muslims which is close to two billion, the Halal market has emerged as one of the most lucrative and influential market globally. Malaysiaâ&#x20AC;&#x2122;s strength is JAKIM ,Thailand is Processed Meat, Indonesia is the huge Muslim population and large livestock industry, Philippine, the strong tie to US market and modern processing technologies availability, Singapore, its strong trading & export link globally. Other members can enlighten their resources in relation to the Livestock industry or human resource expertise to explore the potential Halal meat market. Growing demand for Halal meat in ASEAN is also largely influenced by government policy, politics, religions, customs, traditions and other factors. In the matter of the Halal Meat industry itself AEC must come to a common stand on Quality and Standards set by world bodies on the production and processing of Halal meat and food products. For example, in the matter of ingredient technology and food additives applied in the processing of meat, discoveries of new ingredients have become more complex and complicated beyond the perception of consumers as to what are the inherent original sources and raw materials. Handling of raw materials and processing technologies should ensure that the processing steps and machinery parts in contact with foods are not contaminated with forbidden and harmful substances. The challenges faced by food analyst include unavailability of common officially recognized standard methods among member countries, resulting in different laboratories producing different results and interpretations. Traceability of meat produced at the farm and food additives used in the food industry remain as major hurdles and issues for the Muslim community seeking Halal food from farm to fork. The processes and technological advancements made in raw material processing, ingredient extractions, modifications, purification and re-synthesis, restructured and fabricated into food matrices make the question of traceability and solving the problem of Halalness of the meat and food products and processes a monumental task. There has been increasing level of awareness concerning Halal Halal including foods and beverages, pharmaceuticals, cosmetics or personal care products, which is being driven by increased consumer knowledge of the ingredients used, ways of productions, technically available matured, analytical

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methodologies and alternative consumerâ&#x20AC;&#x2122;s choice of selection. The current techniques in Halal verification for Halal meat products are molecular approaches such as PCR-based techniques. They have demonstrated and proven to be more scientifically, technologically sensitive and high accuracy on authentically of Halal. Those methods are including conventional PCR, real-time PCR and PCR-southern hybridization on DNA chips which are compliance with Syariah laws. AEC must address these issues and compete strategically within members in the spirit of ASEAN.

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Keynote and Invited Abstracts

Applications of Molecular Diagnostic Methods in the Management of Fungicide Resistance Professor Dr. Hideo ISHII Kibi International University, Minami-awaji, Hyogo 656-0484, Japan ABSTRACT

Chemical control is the most common measure to cope with crop diseases in practice. However, development of pathogen isolates resistant to fungicides often causes a failure of disease control. It is therefore essential to monitor the change of fungicide sensitivity in pathogen populations. Cultural methods and/or bioassays using fungicide-treated media and plants, respectively, have been conventionally employed for the monitoring. Recently, some molecular biological methods such as PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and ASPCR (Allele-specific PCR) have been developed and widely used for the diagnosis of resistant isolates of fungal pathogens. In this paper, molecular methods being routinely used for resistance monitoring are introduced. Mode of action and mechanism of resistance have been intensively studied on major group of fungicides. QoI (strobilurin) fungicides have a wide range of spectrum to control fungal and oomycete pathogens on various crops. But, they also carry high risk for resistance development in target pathogens. A point mutation of cytochrome b gene, which encodes fungicide-target protein in mitochondria, resulting in the G143A substitution is the major cause of high QoI resistance. To monitor QoI resistance, PCRRFLP is often used in the rice blast fungus Magnapothe oryzae and other important pathogens. Loop-mediated isothermal amplification (LAMP) offers the potential for accurate and rapid detection of resistance using field samples, even for small diagnostic laboratories. Because the â&#x20AC;&#x153;on-siteâ&#x20AC;? methods are still insufficient, molecular diagnostics have not yet proved useful to growers in guiding in-season of anti-resistance strategies, but further developments in LAMP may overcome this limitation.

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Biogas Reduces the Carbon Footprint of Cassava Starch: A Comparative Assessment with Fuel Oil Dr. Thierry TRAN Food processing & Life Cycle Assessment (LCA) Team Joint Research Unit QUALISUD, French Agricultural Research Centre for International Development, (CIRAD), Montpellier, France Cassava and Starch Technology Research Unit (CSTRU), Kasetsart University, Bangkok, Thailand ABSTRACT

Greenhouse gases (GHG) emissions, or carbon footprint of cassava starch production, were assessed based on three factories in Thailand. The system boundaries included farm stage (production of cassava roots), transportation to factory, processing into native starch. The functional unit (FU) was one ton of native cassava starch at 13% water content. Biogas produced from the factory wastewater was the main source of thermal energy for starch drying (180â&#x20AC;&#x201C;240 m3/FU). Agricultural production contributed approximately 60% of GHG emissions, mainly from the use of nitrogen fertilizers. GHG emissions of root production varied widely due to the diversity of agricultural practices within the same region. The contribution of the factory stage to the carbon footprint depended on the use of electricity, biogas and other fuels, ranging from 200 to 460 kg CO2eq/FU. Allocation rules such as wet-weight or dry-weight basis allocations affected the results markedly. In the past 10 years, 90% of cassava starch factories in Thailand switched from fuel oil to biogas, which reduced GHG emissions of the cassava starch industry as a whole between 700,000 and 1,000,000 tons CO2eq/year.

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O-1

Adapting the CSM-Canegro-Sugarcane model to Simulate Napier Grass in Chiang Mai Province, Thailand Tupthai NORSUWAN1*, Attachai JINTRAWET1 and Carsten MARONH2 1

Center for Agricultural Resource System Research, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand 2 Institute of Plant Production and Agroecology in the Tropics and Subtropics, University of Hohenheim, Stuttgart, Germany *Corresponding email: tupthai.n@cmu.ac.th

ABSTRACT Plot experiment was conducted during November, 2013 to March, 2014 at Chiang Mai province, Thailand. Drip irrigation was applied as Reference Evapotranspiration (ET0) in five-day interval and 300 kg N.ha-1 was applied at two week after planting. Napier grass was harvested three times. Twoweek interval quantification of plant components and literature reviews were used in order to calibrate ecotype and cultivar parameters of the CSMCanegro-Sugarcane model in DSSAT v4.6 Beta. Calibration results displayed that simulated tiller number was corresponded with the observed values as the index of agreement (D-stat, the values varies between zero and one by one being the best fit.) equaled to 0.97, 0.36 and 0.19 in planted crop, the 1st and 2nd ratoon crop, respectively. Maximum radiation conversion efficiency (PARCEmax) was set as 6, 9 and 12 g.MJ-1 in order to find out the best simulation result of total dry matter and partitioning of dry leaf and leaf sheath weight and dry stem weight. Setting PARCEmax as 9 g.MJ -1 gave the best for simulation of planted crop as D-stat ranking between 0.94 to 0.98. In contrast, setting PARCEmax as 12 g.MJ-1 gave the best simulation results on the 1st and 2nd ratoon crop as D-stat ranking between 0.60 to 0.72. Keywords: CSM-Canegro-Sugarcane, Drip irrigation, DSSAT, Napier grass

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Oral Abstracts

Effect of Azolla (Azolla spp.) on Growth and Yield of Pathumthani 1 Rice (Oryza sativa L.) Nattawan BUSSABA1, Phanupong PHONCHAROEN1, and Teerawat SARUTAYOPHAT2* 1

Graduate student of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: kkteeraw@kmitl.ac.th

ABSTRACT Nitrogen is the element that most often insufficient and limited rice yield product while azolla contains the nitrogen-fixing endosymbiont, Anabaena azollae, so it should be used as nitrogen source for rice production. This study was conducted at the experimental field of the Department of Plant Production Technology, Faculty of Agricutural Technology, KMITL, Bangkok during February 4 to May 23 in 2013 to study the effect of azolla and chemical fertilizers on growth and yield of the Pathunmthani 1 rice. Randomized Complete Block Design (RCBD) with 3 replications was used. A 22-day rice seedling was transplanted in 20x25 cm spacing, 2 seedlings/hill with total of 10 hills/plot. Treatments were 9 fertilizer applications as follows: 1) a recommended fertilization (40 kg N/ha and 25 kg P2O5/ha), 2) a non-fertilization, 3) and 4) inoculated azolla 1,250 and 2,500 kg/ha on a transplanting date, 5) and 6) incorporated freshly azolla 6,250 and 12,500 kg/ha into soil for 5 days before transplanting date, 7) incorporated freshly azolla 6,250 kg/ha into soil for 5 days before transplanting with 20 kg N/ha+25 kg P2O5/ha, 8) inoculated azolla 1,250 kg/ha with 20 kg N/ha+25 kg P2O5/ha, and 9) inoculated azolla 1,250 kg/ha with 40 kg N/ha+25 kg P2O5/ha. Result showed that inoculated azolla 1,250 kg/ha with 40 kg N/ha+25 kg P2O5/ha had a significantly higher grain yield than other treatments (p<0.01) of 6,139.0 kg/ha. A recommended fertilization and inoculated azolla 1,250 kg/ha with 20 kg N/ha+25 kg P2O5/ha had an equally grain yield of 5,525.3 and 5,525.2 kg/ha, respectively. A sole inoculated azolla of 1,250 and 2,500 kg/ha had a significantly lower grain yield than a recommended fertilization of 4,691.9 and 4,788.6 kg/ha, respectively. A non-fertilization had a significantly lower grain yield than others treatments of 3,043.8 kg/ha. The results of this study indicated that inoculation azolla on a transplanting date for 1,250 kg/ha can increase grain yield of the Pathumthani 1 rice. Keywords : Azolla, Pathumthani 1 rice, Chemical fertilizer

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O-3

Experiences with Direct Seeded Rice in Jammu and Kashmir, India Vijay BHARTI1*, Ashok KUMAR RAINA2and Anuradha SAHA3 Water Management Research Centre, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus, Chatha, J&K, India-180009 *Corresponding email: vibhrt25@gmail.com

ABSTRACT Rice is main crop during rainy season in plains of Jammu. Transplanting is the dominant rice establishment method under lowland conditions which gave unhusked paddy yield of 4-6 t/ha in coarse varieties and 2.2 t/ha in aromatic basmati varieties. Transplanting requires 20-25 cm of irrigation for puddling so as to create impervious layer to avoid percolation losses. It requires 120 cm of water through 12-14 numbers of irrigations. Well established canal network fixed support price and committed procurement policy of Government of India makes it difficult for the farmers to shift from rice crop. Increase in acreage under rice due to all the positive factors is threatening the most important natural resource-water. Preliminary research investigations conducted worldwide clearly reveal the possibility of direct seeded rice which require discernibly less irrigation water. On similar lines, a study was carried at Research Farm of Sher-e-Kashmir University of Agricultural Sciences of Technology of Jammu during three rainy seasons of 2011, 2012 and 2013 to compare four rice establishment methods in main plots and three irrigation regimes in subplots in split plot design on sandy loam soil. Direct seeded wet sowing rice being statistically at par with direct seeded dry sowing and transplanting showed significantly higher values for growth, yield attributes and yield over System of Rice Intensification (SRI). Irrigation applied at 3 days after disappearance of ponded water recorded significantly higher values for growth and yield parameters as compared to other irrigation regimes. Direct seeded wet sowing rice recorded higher water-use efficiency, highest benefit: cost ratio and net return and hence proved more water saving and rumenerative than other establishment methods. The promotion of direct seeded wet sowing rice is the appropriate option for this region for sustaining rice yield and saving scarce resourcewater. Keywords: Rice establishment methods, Transplanting, Direct seeded rice, Irrigation regimes

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Oral Abstracts

Allelopathic Effects of Pisonia grandis R.Br. on the Germination and Growth of Echinochloa crus-galli (L.) Beauv. Sudteerak SAIPLUEMCHIT1 and Chamroon LAOSINWATTANA1* 1

ABSTRACT Pisonia grandis R.Br. (lettuce tree) is a famous landscaping plant in tropical garden because of a beautiful color and rough texture of leaves. In landscape design, there are various species of plants growing in the same ecology. This study verified the allelopathic effect of lettuce tree dry leaves on germination and seedling growth of Echinochloa crus-galli (L.) Beauv. (barnyard grass) with three experiments conducted in laboratory and greenhouse. First experiment was compared between dry leaf powder and aqueous extract, dry leaf power (125, 250, 375 and 500 mg/plate) was more inhibitory than aqueous extracts at equal concentration. Next experiment was conducted in greenhouse, the comparison of dried leaf powder at different rate of 0, 1, 2, 4 and 6 ton/ ha mixed with sandy loam. It was found that 6 ton/ha gave the significant inhibitory effect on plant height compared with the other concentrations. Last experiment, the dry leaf powder was incorporated into the soil at a rate of 5, 10, 15 and 20 gDW/100gSoil for 0, 1, 2, 3 and 4 weeks. Inhibitory effect on emergence of barnyard grass various depended on the amount of dry leaf powder incorporated and incubating periods. In corporation of dry leaf powder at 15 and 20 gDW/100g soil of 2 weeks showed the most inhibitory effect on emergence of barnyard grass. The results here are providing more knowledge about the allelopathic potential of lettuce tree and can be a useful tip for selecting and grouping the right plants for landscape. Keywords: Allelopathy, Lettuce tree, Germination, Seedling growth, Landscaping plant.

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O-5

How far Teak Plantations can contribute for Climate change Mitigation and Landscape Restoration in Tropics: A case study from Central India Singam Laxmana SWAMY* and Alka MISHRA Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur, Chhattisgarh, India *Corresponding email: swamy_101@ yahoo.com

ABSTRACT One of the major challenges facing by the scientific community of the world today is how to stabilize the increasing levels of greenhouse gases in the atmosphere and reduce the negative impacts of climate change. Intergovernmental Panel on Climate Change (IPCC, 2014) advocated a viable strategy of mitigation and adaptation to climate change through enhancing the carbon (C) pools through via afforestation, reforestation, and agroforestry practices. Tree plantations have great scope and opportunity not only for C sequestration but also restore the degraded landscapes and developing resilient ecosystems. Tectona grandis popularly known as teak, a member of Lamiaceae is grown under commercial plantations in Central and Southern India owing to its excellent quality timber and high economic returns. A study was conducted in age sequence plantations of Teak grown as monoculture and agroforestry in Chhattisgarh state, Central India with an aim to investigate its potential for C sequestration and ameliorating the degraded landscapes. The findings revealed that biomass, C storage and nutrient status (N, P and K) were significantly higher in plantations than agroforestry and further increased by the age. The C storage ranged from 4.5 Mg ha-1 to 62.6 Mg ha- in plantations, and from 2.2 Mg ha-1 to 31.5 Mg ha-1 under agroforestry in 10 year old and 40 year old stands, respectively. Litter fall and N, P and K status were higher in soils under plantations compared to agroforestry. In conclusion, the sole teak plantation should be more beneficial for C sequestration and soil amelioration than agroforestry practices. Keywords: Agroforestry, Biomass, C sequestration, Litter fall, Nutrient storage

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O-6

Antioxidant Properties of Flower Extract of Etlingera elatior (Jack) R.M. Smith Kanokporn CHANGSAWAKE1, Komkhae PILASOMBUT2,Chamroon LAOSINWATTANA1 and Montinee TEERARAK1* 1

Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand. 2 Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand. *Corresponding email: ktmontin@kmitl.ac.th

ABSTRACT Assessment of antioxidant properties of aqueous and ethanol extract from unopened flower of Etlingera elatior (Jack) R.M. Smith (torch ginger) was assayed by using DPPH free radical scavenging assay, metal chelating activity, reducing power, inhibition of lipid peroxidation and total phenolic contents. The results found that the values of half maximal inhibitory concentration (IC50) of DPPH radical were 603.04 ppm in aqueous extract and 2520.61 ppm in ethanol extract while the IC50 values of the reference standard ascorbic acid (Vitamin C) and butylated hydroxyl toluene (BHT) were 3.16 ppm and 21.82 ppm, respectively. In metal chelating activity, the IC50 values were 3689.57 ppm in aqueous extract and 6127.20 ppm in ethanol extract while the IC50 values of the reference standard ethylene diamine tetraacetic acid (EDTA) was 18.32 ppm. Moreover, the ability of reducing power and inhibition of lipid peroxidation increased as the concentrations of aqueous and ethanol extract from Torch Ginger increased. The total phenolic contents recorded were 723.43 and 236.61 mg GAE/100g DW in aqueous and ethanol extract, respectively. The results concluded that aqueous torch ginger flower extract was more potent in DPPH radical scavenger and metal chelating activity than ethanol flower extract. The aqueous and ethanol extract might not be directly involved in reducing power and lipid peroxidation. Keywords: Antioxidant properties, Etlingera elatior, Extract, Total phenolic contents

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O-7

Effect of Storage Conditions on the Vase Life of Homalomena Philodendron and Monstera Nipaporn YONSAWAD1 and Montinee TEERARAK1* 1

Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: ktmontin@kmitl.ac.th

ABSTRACT The objective of this investigation was to study the effect of storage conditions on the vase life of Philodendron (Philodendron spp.), Homalomena (Homalomena spp.) and Monstera (Monstera deliciosa). These cut leaves were placed under three different storage conditions: (i) room temperature (33ºC) with 65% relative humidity (ii) temperature at 20ºC and 88% relative humidity in the dark and (iii) temperature at 20ºC, 88% relative humidity and 12 hrs photoperiod with light intensity of 10. 46 mol m-2s-1. Among the three cut leaf plants studied, Philodendron stored at temperature 20ºC with light had the longest vase life of 27.6 days. Inversely, it had the shortest vase life of 15.6 days under storage of temperature at 20ºC in the dark. Homalomena and Monstera cut leaves had the vase life in the range of 20.42-25.20 days under all storage conditions. Rates of water uptake rapidly declined in all cut leaves stored at 20ºC in the dark. The results of this study suggest that Philodendron cut leaf was more sensitive to postharvest deterioration during storage and a marked reduction of vase life was observed in samples stored at 20ºC in the dark. Keywords: Homalomena, Monstera, Philodendron, Storage condition

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Oral Abstracts

Micro-propagation of Dalbergia cochinchinesis Pierre 1

Wannasiri WANNARAT1*, Panida WONGWEAN , Sirinaree SUPANSOMPORN1, Warinee KITPRECHAWANICH1 and Yupa PANKAEW1 1

Kasetsart Agricultural and Agro-industrial Product Improvement Institute, Kasetsart University, Bangkok, Thailand *Corresponding email: aapwnsr@ku.ac.th

ABSTRACT Dalbergia cochinchinesis Pierre, Siamese Rosewood, is native in Thailand, Lao, Cambodia, and Vietnam. In the past these large evergreen trees were found abundantly in the northeast of Thailand. Today, D. cochinchinesis Pierre is listed as vulnerable species because the tree populations in the natural forest have been declining dramatically due to high demand of limbers in the market. To establish in vitro conservation, the micropropagation of D. cochinchinesis Pierre was investigated in this study. The in vitro shoot clumps were successfully induced from cotyledonary node segments excised from 14-day-old seedlings. The proliferation media supplemented with BA (3.0 mg/l), GA (1.0 mg/l), and NAA (1.0 mg/l) alone or in combination were tested. The highest shoot multiplication was seen when the shoots were cultured on MS containing BA (3.0 mg/l) for 60 days. The shoots rooted on MS medium supplemented with kinetin and IAA after 30-45 days of culture. The plantlets vigorously grew in the soils mixed with sand (1:1) after 30 days of acclimatization. Keywords: Dalbergia cochinchinesis Pierre, Micropropagation, Tissue culture.

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O-9

Department of Fruits, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India 2 Jain Irrigation Systems Ltd, Tamil Nadu, India *Corresponding email: hortidoctorpks@gmail.com

ABSTRACT An experiment was conducted with an objective of assessing the water and nutrient requirement (NPK) through fertigation on physiology, yield and quality of mango cv. Alphonso under ultra-high density planting. There were three irrigation regimes in main plots namely I1 (16 litres per day per plant; lpdp), I2 (20 lpdp) and I3 (24 lpdp) and four fertigation levels in subplots viz., F1 (50% recommended dose of fertilizer; RDF), F2 (75 % RDF), F3 (100 % RDF) and F4 (125 % RDF) replicated three times in a split plot design. The results revealed that among the irrigation treatments I3- 24 lpdp recorded the highest values for physiological traits like chlorophyll a, b and total chlorophyll content, soluble protein, nitrate reductase activity, IAA oxidase activity, carbohydrate content, yield characters like percentage of fruit set, fruit weight, number of fruits per tree, fruit yield per tree and quality characters like TSS, ascorbic acid, sugar, carotenoids. On comparison of sub plot treatments, the physiological parameters were at the highest by application of 125% RDF (F4) through fertigation. The yield contributing factors like percentage of fruit set, mean fruit weight, number of fruits per tree, fruit yield per tree were found to be higher by application of 100% RDF (F3) through fertigation. In the interaction effect, application of 100% recommended dose of fertilizers (120:75:100 g NPK/tree/year) along with 24 lpdp has resulted in the improvement of all beneficial parameters leading to enhanced physiology, yield and quality of mango var. Alphonso under ultra high density planting. Keywords: Fertigation, Irrigation, Physiology, Quality, Yield

July 1-3, 2015

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O-10

Study of Using Coop Fertilizer Ameliorant on Greenhouse Gas Emission in Some Rubber Estates Agroecosystems in Peatland Hariyadi JAMIN PRIYO MINARJO1*, Dedi NURSYAMSI2 and Adi PRADIPTA3 1

Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University (IPB), Jln. Meranti-Dramaga Campus of IPB, Bogor 16680, West Java province, INDONESIA 2 Indonesian Center for Agricultural Land Resources Research and Development (ICALRRD), Jln. Tentara Pelajar 12, Bogor 16114, West Java province, INDONESIA 3 Study Program of Environmental and Natural Resources Management, Bogor Agricultural University (IPB), Baranangsiang Campus of IPB, Jln. Raya Pajajaran Bogor, 16144, West Java province, INDONESIA

*Corresponding email: hariyadiipb@rocketmail.com

ABSTRACT Amelioration is one of effort to increase the peatland productivity that can be applied with due to the environmental aspects. The purpose of this research was to determine the emissions from some rubber agroecosystem in peatlands and their influence on the ameliorant distributions. The research had used PVC pipe that filled with peat, which had previously taken from Jabiren, Central Kalimantan. The research was conducted at the AERC station on June to August 2012. This research consisted of two factors: the type of land use and dose of chicken manure. Types of peatland were consisting of L1 (rubber and shrubs), L2 (rubber and pineapple) and L3 (shrubs). Chicken manure dosage consisted of 0 and 4 tonnes/ha). Gas sampling was weekly taken with close chamber method adopted from IAEA. Sampling of CH4, CO2, and N2O has done manually. Analysis of CO2 and N2O using a GC equipped with a TCD and ECD detector, while CH4 was analyzed using a GC equipped with FID detector. The results showed that ameliorant distribution on land use and the rubber shrubs (L1A2) combined for the highest emissions of CO2 and N2O, which amounted to 2634.66 kg/ha/year and 67.13 kg/ha/year, while CH4 emissions were higher in the treatment without ameliorant. In the land-use of rubber and pineapple (ICCTF), the highest emissions for CH4 and N2O produced by treatment without ameliorant, respectively by 4.41 kg/ha/year and 66.53 kg/ha/year, while the highest CO2 emissions on the same land produced by treatment ameliorant distribution respectively by 2083.32 kg/ha/year. In the shrubs land, CH4 and N2O emissions were also produced at the highest treatment without giving ameliorant at 4.34 kg/ha/year and 9.96 kg/ha/year, respectively. The highest decreased emission in the land-use of rubber and pineapple (ICCTF) amounted to 12.82%. The ameliorant distribution in peatland having been overgrown of shrubs decreased emission to 7.50%. Keywords: Peat, Ameliorant, Methane, Carbon dioxide, Nitrous oxide

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O-11

Plant Nutrient Management Strategies Enhanced Growth, Yield Traits and Mitigated Leaf Reddening in Cotton (Gossypium Hirsutum L.) Allahwadhayo GANDAHI1*, Khalillulah PANHWAR1 and Rabail GANDAHI2 1

Department of Soil Science, Sindh Agriculture University Tandojam, Pakistan 2 Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia *Corresponding Author E-mail: gandahi@yahoo.com

ABSTRACT Cotton leaf reddening is a physiological syndrome which affects the plant growth and crop yields. A field experiment was conducted to study the effect of nutrient management on growth and yield characters of cotton (Gossypium hirsutum L.) at Soil Fertility Section, Agriculture Research Institute Tandojam, Sindh Pakistan (25°25'32"N 68°32'15"E) in a randomized complete block design. Cotton variety Haridost was studied under 4 different rates of NPK (0-0-0- NPK kg ha-1(control), 120-0-0 kg N ha-1, 120-70-0 kg N ha-1, 120-70-40 kg NPK ha-1 and 5 different secondary and micronutrients (Control, 50, 3, 4 and 1.5 kg ha-1 Mg, Fe, Zn and B, respectively) treatments. Results of study revealed that an application of NPK @ 12070-40 kg ha-1 resulted maximum plant height, highest number of green leaves plant-1, greater number of opened bolls plant-1, more total bolls plant-1, seed index, and seed cotton yield ha-1. The treatments without incorporation of K and P (120-70-0 kg ha-1 N-P and 120-0-0 kg N ha-1) resulted in lowest values of all the above traits. Red leaves reduced to 8.43 per plant when soil was fertilized with N, P and K @ 120-7040 kg ha-1; and plots without application of K (120-70-0 kg ha-1) and PK (120-0-0 kg N ha-1) increased number of red leaves. Present investigations further showed that application of micronutrients such as Zn @ 4 kg and B @ 1.5 kg ha-1 were highly effective to control reddening of cotton leaves with 2.43 and 3.88 red leaves plant-1, respectively. Interactive effect of NP @ 120-70 kg ha-1 × Zn @ 4 kg ha-1 resulted in maximum reduction of red leaves plant-1. Regardless the application of N, P or K and their control, reddening of leaves was linearly affected by Zn and B application. The results suggested that leaf reddening problem in cotton will be more severe in soils deficient of Zn and B. It is concluded that there was no direct effect of N, P, K, Mg and Fe on the reddening of leaves in cotton. Plant growth improved markedly when essentially needed NPK was applied @ 120-70-40 NPK in combination with Zn and B @ 4 and 1.5 kg ha-1, respectively. Keywords:

July 1-3, 2015

Cotton, Growth, Leaf reddening, Nutrient management

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Oral Abstracts

Utilization of Calcium Silicate Application on Pepper Seedling Production Eakkarin SUKKAEW1, Suphachai AMKHA1,* Thongchai MALA1 and Pornpairin RUNGCHAROENTHONG2 1

Department of Soil Science, Faculty of Agriculture at KamphaengSaen, Kasetsart University, KamphaengSaen Campus, Nakorn pathom 73140, Thailand 2 Botany, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, KamphaengSaen Campus, Nakorn pathom 73140, Thailand *Corresponding email: agrscak@ku.ac.th

ABSTRACT Silicon can be classified as beneficial element. Many plants show the better growth as the silicon from available (H4SiO4 or SiO42-). The pepper production to silicon in soil Thailand is not cleared. Thus purpose of this research is to study the rate and application method of silicon fertilizer appropriated for pepper seedling production. This study was dividedinto two experiments. Experiment 1 was the effect of calcium silicon (Ca2SiO4) fertilizer application in pepper by soil drench method using 2x6 Factorials in Completely Randomized Design (CRD) with 4 replications. Factor A was seed preparation methods (seed priming with Ca2SiO4 fertilizer at a rate 2 g L-1 and non-seed priming) and factor B was application rates of Ca2SiO4 fertilizer at 0, 30, 60, 120, 240 and 480 kg ha-1. Experiment 2, the effect of Ca2SiO4 fertilizer application in pepper by foliar method was conducted 2x6 Factorials in Completely Randomized Design CRD with 4 replications. Factor A was seed preparation methods (seed priming with Ca2SiO4 fertilizer at a rate 2 g L-1 and non-seed priming) and factor B was application rates of Ca2SiO4 fertilizer at 0, 2, 4, 6, 8 and 10 g L-1. All experiment data were collected such as plant growth (plant height, leaf number, fresh weight, dry weight) and total silicon content in plant at 28 days after sowing (DAS). From the 1stexperiment, the results showed that utilization of Ca2SiO4 fertilizer application by seed priming method at a rate 2 g L-1 with Ca2SiO4 fertilizer application by soil drench at a rate 120 kg ha-1 gave the highest plant growth and total silicon in plant. Regard to the 2nd experiment, the utilization of Ca2SiO4 fertilizer application by seed priming method at a rate 2 g L-1 with Ca2SiO4 fertilizer application by foliar at a rate 2 g L-1 gave the highest plant growth and total silicon in plant. To conclude, Ca2SiO4 fertilizer application can be employed for enhancing plant growth of pepper seedling and increasing silicon content in plant. However, the utilization of Ca2SiO4 fertilizer application at a high dose in the pepper seedling could decrease plant growth. Keywords: Foliar, Fertilizer, Pepper seedling, Silicon, Soil drench

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2 International Symposium on Agricultural Technology (ISAT2015)

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O-13

Behavior of Nutrient Uptake by Pummelo Growing on Salt Marsh Soil Hien HUU NGUYEN1*, Somsak MANEEPONG1 and Potjamarn SURANINPONG1 1

School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, Thailand *Corresponding email: huuhiendhv@gmail.com

ABSTRACT Pummelo growing on salt marsh soil is more superior taste than the same variety growing on other soils. This study aimed to examine soil chemical properties and their contribution to nutrient concentrations in leaves. Soil samples were taken under pummelo canopies at depth of 0-20 cm. Leaf samples of 3-5 month old were taken from beneath canopies of the same trees. Soil chemical properties and nutrient concentrations in the leaves were determined. Soil pHs were slightly alkaline (7.10.3) and their electrical conductivities were slightly saline (2.70.9 mS/cm). Concentrations of P in the soils (17794 mg/kg) were much higher than its optimum range (15-25 mg/kg), whereas the concentrations in leaves (1.40.1 g/kg) were at lower marginal of its optimum range (1.5-2.0 g/kg). Excess P in soils did not cause luxury consumption. A mole ratio of Ca/K in soils (3.61.7) was higher than that of in leaves (2.30.9). K can be uptaken better than Ca, although it had lower concentration than Ca in the soils. K also can be uptaken better than Mg, since K/Mg in the soils (0.40.2) was lower than that of in the leaves (1.50.3). A mole ratio of Ca/Mg in soils was lower than that of in leaves, indicating that Ca is able to be uptaken better than Mg. Concentrations of K, Ca and Mg in soils were higher than the optimum ranges, but only Mg concentration in leaves was higher than the optimum ranges. Antagonism effect of Mg was shown to inhibit the uptake of K and Ca, and cause excess consumption of Mg. Keywords: Nutrient uptake, Plant nutrition, Pummelo, Salt marsh

July 1-3, 2015

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2 International Symposium on Agricultural Technology (ISAT2015)

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O-14

Eco-Friendly Utilization of TDE and Fertilizers on Soil Properties, Yield and Quality of Seed Cane J. REVATHI1 and M. BASKAR 1

Research Scholar, Department of Soil Science and Agricultural Chemistry Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India *Corresponding email: revagrichem@gmail.com

ABSTRACT An experiment was conducted with an objective of assessing the utilization of TDE and fertilizers on soil properties, yield and quality of seed cane. There were four main plots viz., M1 (control), M2 (TDE at a dilution 1:10 on 75th days after planting), M3 (TDE at a dilution 1:10 on 75th and 120th DAP) and M4 (TDE at a dilution 1:10 on 75th, 120th and 165th DAP) and five fertilizer treatments in subplots viz ., S1 (control), S2 (100 % NPK), S3 (100 % NP), S4 (75 % N and 100 % P) and S5 (75 % NP) replicated three times in a split plot design. Results revealed that among the main plot treatments application of TDE at a dilution 1:10 on 75th, 120th and 165th DAP (M4) recorded highest soil chemical properties like available N, P, K, exchangeable cations, micronutrients, yield (33.7 % over control) and quality. Among sub plot treatments, same results were recorded by application 100 % RDF (S2). On interaction, application of three times TDE along with 100 % recommended dose of fertilizers (225:62.5:112 kg NPK/ ha /year) has resulted in the improvement of soil properties leading to enhancement of the seed cane yield, quality and it was on par with three times application of diluted TDE with 75 % NP fertilizer. It is noteworthy that, by omitting 100 % K and 25 % NP, in combination with three times of diluted TDE improved yield and quality of seed cane. Keywords: Fertilizers, Seed Cane, Soil Properties, TDE, Yield

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O-15

A Co-Culture System of Giant Tiger Prawn and Unexploited Seaweed and Snail Isao TSUTSUI1,2,*, Dusit AUE-UMNEOY2, Jaruwan SONGPHAT KAEW2, Piyarat PINPHOO2, Chonlada MEEANAN3, Sirimas KLOMKLING3, Halethichanok SUKCHAI3, Monthon GANMANEE2, and Kaoru HAMANO4 1

Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, Japan 2 King Mongkut’s Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand 3 Shrimp Co-culture Research Laboratory (SCORL), KMITL, Bangkok, Thailand 4 Research Center for Marine Invertebrates, National Research Institute of Fisheries and Environment of Inland Sea, Onomichi, Hiroshima, Japan *Corresponding email: cong@affrc.go.jp

ABSTRACT Shrimp aquaculture is an important industry in the world, especially southeast Asian countries. In this presentation, we introduce the basic concept for our shrimp co-culture system and some of the advantages of this co-culture system for sustainable low cost shrimp culture for small scale shrimp aquaculturists in southeast Asian countries. Chaetomorpha sp. and Stenothyra sp. are eulyhaline species, and they are considered to be easily applied for blackish shrimp aquaculture. Especially Chaetomorpha sp. exhibited 100% survival over the range of 0.5–80 psu and 15–35°C in laboratory experiments. The highest mean growth rates of Chaetomorpha sp. were observed in salinities of 20 psu and 30 psu, at a temperature of 30°C. The results in the laboratory experiment revealed that the mean SGRs of cocultured shrimp were significantly higher than mono-cultured shrimp for all periods of the experiment. The Feed conversion ratio was reduced and 38.9 % lower in co-culture than in monoculture tanks. Keywords: Chaetomorpha, Feed conversion ratio reduction, Growth enhancement, Shrimp co-culture, Stenothyra

July 1-3, 2015

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Oral Abstracts

Investigating the Safety of Potential Probiotic Candidates Isolated from the GI Tract of Tilapia in vitro Rungtawan YOMLA1,2, Daniel MERRIFIELD1 and Simon DAVIES1 1

Aquatic Animal Nutrition and Health Research Group, Portland Square A425, Plymouth University, Devon, PL4 8AA, UK 2 Faculty of Agricultural Technology, KMITL *Corresponding email: rungtawan.yomla@plymouth.ac.uk; kprungta@kmitl.ac.th

ABSTRACT Thrity-four bacterial colonies were isolated from the intestinal tract of nineteen tilapia collected in different cultures in Thailand. Fifteen intestinal bacteria were able to inhibit the growth of pathogenic bacteria: Aeromonas hydrophila and Streptococcus iniae. Bacterial isolates were evaluated for safety as probiotic candidates by testing antibiotic resistance and blood hemolytic activity. A number of bacterial isolates, when concentrated at more than 1014 CFU mL-1, displayed resistance to antibiotics. Macrococcus caseolyticus (isolate 2) was susceptible to all antibiotics tested. Three isolates [Staphylococcus arlettae (isolate1), Enterobacter sakazakii (isolate 7), and En. asburiae (isolate 33)] displayed resistance to erythromycin. Four bacterial isolates (Bacillus sp. (isolate 14), and Ba. cereus (isolates 15, 18, & 23) displayed resistance to sulphamethoxazole/thrimethoprim. Multiantibiotic resistance was observed by Bacillus sp. (isolate 18), which displayed resistance to ampicillin, cephalothin, and sulphamethoxazole/thrimethoprim. A number of Bacillus isolates [Bacillus sp. (isolate 14), Bacillus sp. (isolate 18), and Ba. cereus (isolates 15, 17, 23)] displayed positive blood hemolysis. The isolates, which do not display antibiotic resistance or hemolytic activity, will be tested in in vivo trials by using adhesion properties, and resistance to gastric conditions. And then, potential probiotic candidates will be selected using multi-parameters in vivo trials to quantitative data with weight score assumptions. Then, high potential probiotic candidates will be studied in with expectation of elucidating a novel potential probiotic for tilapia application. Keywords: Tilapia, Intestinal bacteria, Potential bacterial probiotic, Antibiotic resistances, Blood hemolysis

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O-17

Studied on Using of Robo-Logger Technique Measuring In Situ Inside Shell Temperature of Rocky Shore Limpet Monthon GANMANEE1*, Chayanid MEEPOKA1, Pokamon KOCHARIN1 and Gray A. WILLIAMS2 1

Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Swire Institute of Marine Science, University of Hong Kong, Pokfulam Road, SAR China *Corresponding email: kkmontho@kmitl.ac.th

ABSTRACT Temperature is one of the most important environmental factor driven distribution pattern and physiological traits of intertidal rocky shore organisms. However, it is difficult to understand their body temperatures under in situ conditions. In this study, the robo-logger technique was used by putting thermo-logger (waterproof device model Thermochron Maxim DS1922) inside limpet shell (Cellana toreuma) filled with ScotchcastÂŽ 2131. The robo-loggers were deployed at 3 tidal levels (high, mid and low shore) in 2 sites (intertidal rocky shore near Asadang Bay, Sichang Island, Chonburi province and Muang district, Chumporn province). Each tidal level, a pair of robo-logger was deployed in order to determine effect on temperature variation in different degree of rock surface. One of robo-logger was put on vertical rock surface or crevice and the other was put on flat rock surface. Temperature inside was recorded every 30 minutes for 9 months (August 2014 to April 2015). The data will be compared with hourly air temperature data obtained from local meteorological station. Spatial and temporal variation of in-shell temperature will be determined. Keywords: Climate change, Limpet, Robo-Logger, Rocky shore, Temperature

July 1-3, 2015

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Oral Abstracts

Impacts of the PTT GC Oil Spill on Intertidal Rocky Shore Macrobenthos in Ao Prao, Samed Island Jindarha PREMPRAMOTE1*, Chayanid MEEPOKA1, Sujitra SAMAKRAMAN1 and Monthon GANMANEE1 1

Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520 Thailand *Corresponding email: j_aoei@hotmail.com

ABSTRACT On 28 July 2013, 50,000 litres of the crude oil leaked from a pipeline owned by PTTGC Plc, and burst while oil was being transferred from an undersea well to a tanker at Rayong Province, Thailand. The oil spill moved eastward and reached rocky shore and beach at Ko Samet's Ao Phrao in the following day resulted in serious impact to the shore. Abundance (in density), diversity and community structure of macrobenthos at rocky intertidal of Ao Phrao were monitored 3, 6 and 9 months compared with unaffected reference sites. Transects were positioned at five different heights from low to mid shore (1.5 to 2.5 m above MTL). At each height, abundance of rocky shore macrobenthos was recorded within 30 replicates quadrats (25 x 25 cm). Decreases in abundance and diversity indices were observed for rocky shore macrobenthos at Ao Phrao whilst these parameters were not significantly different in reference site (p<0.05). Results in MDS (multidimentional scaling) revealed zonation pattern of rocky shore macrobenthos in Ao Phrao did not change or move up to higher shore. This finding suggested that intertidal rocky shore macrobenthos were impacted by the oil spill. Long term monitoring program should be carried out to clarify abundance and community structure of these organisms to restore to its original level before oil spill. Keywords: Community structure, Intertidal rocky shore, Macrobenthos, Oil spill, Samed Island

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A Study on Some Production Traits and Egg Quality Characteristics of Lutein Eggs Kanda LOKAEWMANEE1* and Supat DUANGDEE1 1

Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand *Corresponding email: csnkdp@ku.ac.th

ABSTRACT This investigation was carried out to demonstrate some production traits and egg quality characteristics of lutein eggs. One hundred twenty laying hens were used in the study. The birds were fed a basal mash diet supplemented with lutein at 0 (CON) and 50 mg/kg (LUT) for three weeks. Birds had free access to feed and water throughout the trial, and egg production were recorded weekly. Thirty eggs per group were evaluated weekly for egg quality characteristics such as yolk weight, albumen weight, shell weight, shell thickness, Haugh unit, shape index and albumen index. The egg yolk color was visually examined using the Roche Yolk Color Fan. The egg yolk color was further examined for chroma using a spectrophotometer, an objective method. The deposition of lutein into egg yolk was indentified at the end of the experiment. There was no significant difference in final body weight, the relative live weight, feed consumption, hen-day egg production, egg mass, feed efficiency, egg weight, yolk weight, albumen weight, shell weight, shell thickness, Haugh unit, shape index, albumen index and yolk index among dietary groups. The values of yolk color, chroma and concentration of lutein increased in raw and boiled egg yolk in the LUT group compared with the CON group (P<0.05). In conclusion, the present study might suggest that lutein at 50 mg/kg of feed enhanced raw and boiled egg yolk color by increasing chroma value and improving concentration of lutein in egg yolk compared with the lutein at 0 mg/kg of feed. Keywords: Production traits, Egg quality Characteristics, Lutein egg

July 1-3, 2015

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2 International Symposium on Agricultural Technology (ISAT2015)

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O-20

Effect of Leucaena Silage Levels on Rumen Fermentation and Nutrients Digestibility in Dairy Steers Giang NGUYEN THIEN TRUONG1 and Metha WANAPAT1* 1

Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand *Corresponding email: metha@kku.ac.th

ABSTRACT The objective of this experiment was to determine effects of Leucaena silage (LS) levels on rumen fermentation and nutrients digestibility in dairy steers. Four, rumen fistulated dairy steers with initial weight of 167 Âą 12 kg were randomly assigned to receive diets according to 4x4 Latin square design. Treatments were: T1 = untreated rice straw (Control), T2 = 70% untreated rice straw + 30% LS, T3 = 40% untreated rice straw + 60% LS and T4 = 100% LS. All treatments were fed ad libitum intake. Results revealed that dry matter intake and digestibility were linearly increased together with increasing levels of LS (P<0.05). Ruminal pH was not changed among treatments while ruminal NH3-N concentration and bacterial population were enhanced with increasing levels of LS (P < 0.05). The blood urea nitrogen was in normal ranges. The acetic acid concentration was decreased (P < 0.05) while propionic acid concentration was increased (P < 0.05) in all LS levels, especially with full LS feeding. Based on this study, it could be concluded that LS has improved digestibility of nutrients, rumen microbial population and rumen fermentation, and the highest improvement was at 100% LS level. Keywords: Dairy steer, Leucaena silage, Rumen fermentation, Rice straw

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O-21

Effect of Dried Leucaena Leaf and Leucaena Silage Supplementation on Nutrients digestibility, Rumen Fermentation in Swamp Buffalo Kampanat PHESATCHA1and Metha WANAPAT 1* 1

ABSTRACT The experiment was conducted to determine the effect of dried Leucaena leaf (DLL) and Leucaena silage (LS) supplementation on rumen fermentation and microbial protein synthesis in swamp buffalo fed rice straw. Four, rumen-fistulated swamp buffaloes were randomly assigned according to a 4Ă&#x2014;4 Latin square design under four dietary treatments; unsupplementation (control), supplementation with DLL at 4 kg/hd/d, supplementation with DLL at 8 kg/hd/d and supplementation with LS at 8 kgDM/hd/d. Untreated rice straw was fed ad libitum, while concentrate was fed at 0.1% BW. The results revealed that total intake was significantly improved when levels of DLL and LS supplementation increased. Ruminal ammonia nitrogen (NH3-N) concentration was significantly increased by DLL and LS at high level of supplementation. There was an increase (P<0.05) in total volatile fatty acid (TVFA), propionic acid (C3), while the highest were found in LS supplemented group. Total urinary dihydroxy pyridine (DHP) corresponded with the mimosine intake. In addition, hormonal profile in this study revealed that, the level of thyroxine (T4) was increased by DLL and LS supplementation while triiodothyroxine (T3) were not changed by treatments. Based on this study, it could be concluded high level of DLL supplementation at 8 kg/hd/d and LS at 8 kgDM/hd/d could enhance the nutrients digestibility, rumen fermentation in swamp buffaloes fed rice straw without adverse effect. Keywords: Leucaena leucocephala, Rumen ecology, Ruminant, Silage

July 1-3, 2015

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O-22

Supplementation of Urea on Nitrogen Balance and Microbial Protein Synthesis in Swamp Buffaloes Fed On Cassava Hay and Rice Straw Thiwakorn AMPAPON1, Metha WANAPAT1* and Kampanat PHESATCHA1 1

ABSTRACT The experiment was conducted using four, rumen-fistulated swamp buffaloes with initial average liveweight 355Âą15.0 kg, to study urea (NPN) and cassava hay supplementation on feed intake, digestibility, rumen fermentation and microbial protein synthesis in swamp buffaloes. Treatments were as follows: T1= supplementation with cassava hay at 400 g/hd/day without urea (Control), T2= supplementation with cassava hay at 400 g/hd/day + urea 30 g/hd/day, T3= supplementation with cassava hay at 400 g/hd/day + urea 60 g/hd/day, T4= supplementation with cassava hay at 400 g/hd/day + urea 90 g/hd/day. All buffaloes were randomly allocated to receive diets according to a 4 Ă&#x2014; 4 Latin square design. The present results revealed that Total dry meter intake were significantly increased level of urea (P<0.05). While, purine derivative (PD) excretion, PD absorption, N absorption, MNS and EMPS were significantly increased at 60 and 90 g/hd/d level of urea (P<0.05). Moreover, bacterial population particularly those of proteolytic, cellulolytic and amylolytic bacteria were linearly increased with increasing level of urea (P<0.05). Based on this experiment, the results revealed that supplementation of urea between 60 to 90 g/hd/d with cassava hay at 400 g/h/d resulted in improved microbial protein synthesis in swamp buffaloes fed on rice straw. This result implies the beneficial feeding of non-protein nitrogen along with low-quality roughages especially in the tropics. Keywords: Microbial Protein Synthesis, Rice Straw, Swamp Buffaloes, Urea

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O-23

Effects of Drying Process on Characteristics of Gac Fruit Aril Powder Warangkana ANGKANANON1, Mudtorlep NISOA2, Phanphen WATTANARSAKIT3, and Visaka ANANTAWAT1* 1

School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, Thailand 2 School of Science, Walailak University, Nakhon Si Thammarat, Thailand 3 Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand *Corresponding email: anvisaka@gmail.com

ABSTRACT Gac fruit (Momordica cochinchinensis Spreng) aril gains high attention from consumers due to its functional properties. The objective of this research is to investigate the effects of drying process on gac fruit aril powder characteristics. The aril extract was dried using hot air, spray, freeze and microwave driers. The powders were analyzed for physicochemical and microbiological properties. Drying equipment and condition had significant effects on colour, aw, lycopene, β-carotene, antioxidant activity and microbiological properties of gac fruit aril powder. The lycopene, βcarotene, antioxidant activity and aw of gac fruit aril powder spray dried at 170 ºC with 0-30% maltodextrin addition were in the range of 0.48 - 1.32 mg/g, 0.3-0.93 mg/g, 1115657 - 1284696 µmol Trolox/g and 0.396 - 0.464, respectively. The gac fruit aril powder freeze dried at a condenser temperature of -80ºC and pressure of 0.045 mbar for 72 h contained 1.13 mg lycopene/g, 0.96 mg β-carotene/g, and antioxidant activity of 1145810 µmol Trolox/g. Hot air drying at 60, 70 and 80 ºC resulted in the powders with 1.05 - 1.10 mg lycopene/g, 0.33 - 0.38 mg β-carotene/g, and antioxidant activity of 1211285 - 1284696 µmol Trolox/g. Whereas, microwave drying at 185 W was not a successful drying method for gac fruit aril powder. Considering the powder characteristics and quality as well as the drying cost, the suitable condition for gac fruit aril powder production was spray drying at 170ºC with 10% maltodextrin as a drying carrier. Keywords: Antioxidant, Carotene, Drying, Gac Fruit, Lycopene

July 1-3, 2015

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Oral Abstracts

Modeling the Microwave Heat Distribution of Banana at Different Ripening Stage W. THUTO and K. BANJONG* Faculty of Agro-Industry, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: kbkittic@kmitl.ac.th

ABSTRACT Microwave heating may be a viable alternative to maintain fruit quality, as a new thermal treatment method for postharvest insect control or reduce postharvest disease in agricultural commodities. Banana is one of the important high sugar containing tropical fruit crops grown commercially in many countries. This study focused on the influence of sugar content in banana (Musa (ABB group) "Kluai Nam Wa") on heat distribution during microwave heating. In this regard, COMSOL Multiphysics was used to simulate the three-dimensional computer model based on FEM and it satisfactorily predicted the temperature distributions inside the banana slices. This model was validated by comparing with temperature data obtained by an infrared thermometer. Three different ripening stages of banana were investigated and the banana samples were heated by microwave at 800 W for 1 minute. The results showed that sugar content as the effect of the response variable. Initially, the hot spot was generated in the core of the banana sample and subsequently the temperature was distributed radially outward. The banana samples with high sugar content showed higher temperature increases due to their greater dielectric loss factor and the temperature gradually increased to higher levels over time compared to those with low sugar content. The predicted temperature well agreed with the experimental data obtained from an infrared thermometer. Keywords: Heat distribution, Microwave heating, Modeling, Banana, Multiphysics

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O-25

Preparation, Optimization and Characterization of Carboxymethyl Cellulose from Rice Straw Using Microwave Heating Noppadol PANCHAN and Chalida NIAMNUY* Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok, Thailand *Corresponding email: fengcdni@ku.ac.th

ABSTRACT Rice straw is an abundant agricultural waste, which can be useful resource as it contains approximately 35% cellulose. Sodium carboxymethyl cellulose (CMC) is the most important water-soluble derivative form of cellulose, which is used in several industrial fields. Therefore, the utilization of rice straw for preparation of CMC is of interest. Due to the preparation processes of CMC requiring high temperature for a long reaction time for the conventional heating, the microwave heating is a high performance alternative technique because it can shorten the process time and environmental friendly. The aims of this work are to isolate Îą-cellulose from rice straw, which is the raw material for CMC preparation and to investigate the effect of various parameters such as sodium hydroxide (NaOH) concentrations (5, 20 and 35 g/100 ml), sodium chloroacetate (SCA) doses (2, 8 and 14 g), microwave power (160, 320 and 480 W) and etherification times (1, 5 and 9 min) on degree of substitute (DS) of prepared CMC. In addition, the experimental condition was designed using Box-Benhken experimental design. Response surface methodology (RSM) is applied to optimize the CMC preparation process, with the best condition for preparation by using 5.505 g/100 ml of NaOH concentration and 12.607 g of SCA dose with etherification at 320 W of microwave power for 7.97 min. Under this condition, CMC with DS 0.697 was produced. The Fourier transform infrared (FT-IR) spectroscopy and X-Ray diffraction (XRD) spectroscopy are also used to characterize the CMC product. Keywords: Box-Benhken experimental design, Carboxymethyl cellulose, Etherification, Microwave heating, Response surface methodology, Rice straw

July 1-3, 2015

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Oral Abstracts

Optimization of Process Parameters on the Production of Bacterial Cellulose from Rice Rinsing Waste Water (nata-de-leri) by Acetobacter xylinum Alwani HAMAD*, Giswantara and Endar PUSPAWININGTYAS Department of Chemical Engineering, Faculty of Engineering, Muhammadiyah University of Purwokerto, Dukuh Waluh Rd, PO BOX 202 Purwokerto, Central Java 53182, Indonesia *Corresponding email :hamadalwani@yahoo.co.id

ABSTRACT Nata is a bacterial cellulose that derived from the fermentation of Acetobacter xylinum. It is often found in desserts as healthy functional foods because of a lot of fiber. Not only it is produced from coconut water known as nata-de-coco, but also it could be produced from other sources such as waste-water from rice rinsing as nata-de-leri. The waste-water is produced from rice rinsing still contains carbohydrate source that can be used as substrate of Acetobacter xylinum fermentation. This study aims to optimize paramaters of the fermentation of bacterial cellulose (nata-de-leri) using statistical method (Placket Burman Method and Response Surface Method) in order to enhance production. The optimization strategy in this study through statistically designed experiments as an effective tool for fermentation engineering. First, Plackett–Burman screening design was applied to address the most significant parameter components affecting nata-de-leri production. A large of continous factors (ratio between rice and water, source of carbon using sucrose, source of nitrogen using CO(NH2)2, source of phosphate using K2HPO4, pH, vitamin, fermentation time, concentration of starter) were screened and insignificant ones were eliminated in order to obtain a smaller, more manageable set of factors. The two-level Plackett–Burman design was used under our experiment at static culture condition at 28–30oC. Second, Response surface method using Central Composite Design was employed to find out the optimal levels of fermentation parameters resulted from the first step using response of yield. The result of this study showed that concentration of sucrose, concentration of CO(NH2)2, concentration of starter and fermentation time were found to be the major factors of the nata production. Estimated optimum values for the production of nata-deleri were as follows concentration of sucrose were 17.5 g/ml; concentration of CO(NH2)2 were 12 g/ml; concentration of starter were 20%; fermentation time for 12 days. It resulted yield of nata at 97.20%. From this study, we can conclude that the optimum nata-de-leri yield was resulted from optimization which was 20 % bigger than the basal parameters. Keywords: Bacterial cellulose, Nata De Leri, Acetobacter xylinum, Rinsing rice waste water, Fermentation

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O-27

Orthogonal Functions for Mathematical Modeling in Problems on Agriculture Technology Mohsen RAZZAGHI* Department of Mathematics and Statistics, Mississippi State University, Mississippi State, MS 39762, USA *Corresponding email: razzaghi@math.msstate.edu

ABSTRACT The available sets of orthogonal functions can be divided into three classes. The first includes a set of piecewise constant basis functions (PCBF's) (e.g., Walsh, block-pulse, etc.). The second consists of a set of orthogonal polynomials (e.g., Laguerre, Legendre, Chebyshev, etc.). The third is the widely used set of sine-cosine functions in Fourier series. While orthogonal polynomials and sine-cosine functions together form a class of continuous basis functions, PCBF's have inherent discontinuities or jumps. For mathematical modeling in problems on agriculture technology, images often have properties that vary continuously in some regions and discontinuously in others. Thus, in order to properly approximate these spatially varying properties, it is necessary to use approximating functions that can accurately model both continuous and discontinuous phenomena. Therefore, neither continuous basis functions nor piecewise constant basis functions taken alone can accurately model these spatially varying properties. For these situations, hybrid functions, which are the combinations of piecewise and continuous functions, will be more effective. The solution of radiative transfer equation (RTE) has wide applications in mathematical modeling for problems on agriculture technology, environmental modeling, and remote sensing. In this work, we present a new approach to the solution of RTE. Our approach is based upon hybrid functions, which are combinations of blockpulse functions and continuous functions. Numerical examples are included to demonstrate the applicability and the accuracy of the proposed method, and a comparison is made with the existing results. Keyword:

July 1-3, 2015

Agriculture technology, Environmental modeling, Mathematical modeling, Orthogonal functions, Remote sensing

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Oral Abstracts

Sensitivity Detection of Phytophthora spp. Causing ParaRubber Leaf Fall Disease to Some Systemic Fungicides Pornprapa KONGTRAGOULl*and Panot VIRIYAEKKUL1 1

Program in Horticulture, Division of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Prince of Chumphon Campus, Chumphon, Thailand * Corresponding email: kkpornpr@kmitl.ac.th

ABSTRACT Leaf fall disease of para-rubber was diagnosed by tissue transplanting technique. The 12 isolates of oomycetes were obtained and then observed for morphological characteristics by growing on V8 agar for 5 days. All isolates showed white colony which the growth rate of the colonies was 14.54Âą1.46 mm/day. The mycelia showed hyaline branching non-septate hyphae, and formed sporangium. These morphological characteristics were identified as Phytophthora sp. The resistibility to some systemic fungicides including dimethomorph, fosetyl-Al, and metalaxyl were detected from the growth of colony by poisoned food technique. The level of resistance to each fungicide was evaluated and grouped into 2 representative phenotype reactions where fungicide resistance (FgR) was for the isolate that be able to grow on each fungicide at more than, or equal to the field-use recommended rate and fungicide sensitivity (FgS) for the isolate that could grow on at less than the field-use recommended rate. Nine isolates were classified as the FgR to metalaxyl. However, all isolates were classified as the FgS to dimethomorph and fosetyl-Al. Keywords: Dimethomorph, Fosetyl-Al, Metalaxyl, Para-rubber, Phytophthora sp.

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Oral Abstracts

O-29

Assessment of the Antagonistic Activity of Trichoderma spp. from Five Different Habitats on Plant Pathogenic Fungi Suriyasit SOMNUEK1*, Pornprapa KONGTRAGOUL2 and Tanimnun JAENAKSORN1 Department of Plant Production Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok, Thailand 2 Program in Horticulture, Division of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Prince of Chumphon Campus, Chumphon, Thailand *Corresponding email: suriyasitsom@gmail.com

ABSTRACT The antagonistic efficacy of Trichoderma spp. from five different habitats (T121Kh, T114Kb, T114So, T112Sc and commercial Trichoderma) were determined on mycelial growth of six plant pathogenic fungi, namely Alternaria sp., Curvularia sp., Fusarium sp., Helminthosporium sp., Pestalotia sp., and Rhizoctonia sp. by dual culture test. The results showed that all tested Trichoderma spp. had potential to inhibit the mycelial growth of all tested pathogens. T114Kb showed the highest growth inhibition on Alternaria sp., Curvulari sp., Fusarium sp., Helminthosporium sp., and Rhizoctonia sp. in the range of 67.40-77.77 percent and followed by commercial Trichoderma with 64.81-77.40 percent inhibition against Alternaria sp., Curvularia sp., Helminthosporium sp. and Rhizoctonia sp. Regarding antagonistic mechanism, antibiosis and competition mechanism were shown. Therefore, these tested Trichoderma spp. can be used for further studies on in vivo to confirm the feasibility of using in plant disease control. Keywords: Trichoderma spp., antagonists, plant pathogenic fungi

July 1-3, 2015

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O-30

Colonization of Plant Root and Punctured Surface Tissue by Non-Pathogenic and Pathogenic Fusarium oxysporum Titi THONGKAMNGAM* and Tanimnun JAENAKSORN *Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: Titi29950@hotmail.com

ABSTRACT Purpose of this research was to assess the colonization of non-pathogenic Fusarium oxysporum strain F221-B and pathogenic F. oxysporum strain F221-R and F422-G on roots of lettuce (Lactuca sativa L.) grown in hydroponics and on punctured surface tissue of pakchoi (Brassica chinensis var. chinensis). The colonization was assessed in terms of epiphytic and endophytic colonization. Besides, survival of the tested fungi in nutrient solution was also determined. The results showed that non pathogenic F221B could colonize on root surface (as epiphytic colonization with 28.5 and 54 CFU/ml in the 1st and 3rd week after inoculation, respectively) as well as inside the root (endophytic colonization was about 60 CFU/ml in the 1st wk. and 76 CFU/ml in the 3rd wk after inoculation) without producing any disease symptom. Unexpectedly, the pathogenic F221-R and F422-G were found to colonize only on root surface as epiphytic colonization. Regard to the colonization on punctured surface tissue, the top-view as well as crosssectional view of colonization of plant surface tissue and top-view of epidermal peel of a plant leaf were visualized and recorded. Keywords: Non-pathogenic Fusarium oxysporum F221-B, Epiphytic colonization, Endophytic colonization, Lactuca sativa L., Brassica chinensis var. chinensis

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O-31

Integrated Management Yellow Vein Mosaic Disease of Okra C.P.KHARE1*, A. KOTESTHANE, D. SHARMA2, A. DIXIT2 and J. SINGH2 1* 2

Department of Plant Pathology, Indira Gandhi Agricultural University Raipur â&#x20AC;&#x201C; 492006 (Chhattisgarh) India Department of Vegetable Science, Indira Gandhi Agricultural University Raipur â&#x20AC;&#x201C; 492006 (Chhattisgarh) India *Corresponding email: dr.cpkhare@rediffmail.com

ABSTRACT Okra is a multipurpose crop due its diversified uses. Diseases are the key factors affecting its cultivation in India and Yellow vein mosaic disease is major among them. The disease causes enormous losses both qualitative and quantitative. Considering the importance of disease, its proper management, the present study was undertaken in two parts at Indira Gandhi Agricultural University, Raipur (Chhatisgarh) India in Kharif/rabi season of 2012-13. In the first part, a trial was conducted in RBD with three replications with the plot size 4.8 x 2.7 m2 and the spacing 45 x 30 cm. The crop was raised with good agronomic practices and insecticides alongwith neem oil and neem oil (alone) were evaluated against the disease. They were sprayed four times in the crop at 10 days interval and observations of disease were recorded in 15 days interval after the appearance. In the second part, resistant seventeen entries (AVT-I & AVT-II group) were evaluated against the disease. The trial was conducted in RBD with three replications and planting was done. The plot size used was 4.8 x 2.7 m2 with the spacing 45 x 30 cm. the crop was raised with good agronomic practices and observations of disease incidence were recorded in 15 days interval. The lowest disease incidence was recorded by spraying of Acephate 75% SP @ 1.5 gm/lit+ Neem oil 0.15% EC @ 2.0 ml/lit followed by Imdacloprid 17.80% SL @ 0.5 ml/lit+ Neem oil 0.15% EC @ 2.0 ml/lit followed by Admire @ 2 g/l+ Neem oil and Hostothion + Neem Oil at 10 days interval. Out of 17 entries evaluated under various groups two (2012/OK YVRES 5and 2012/OKYVRES-6), were free from the disease from each group i.e. AVT-I& AVT-II. One entry (2012/OK YVRES 2) from AVT-I group was highly resistant to the disease. The insecticides play a significant role in the management of the disease by reducing whitefly population and incidence of the disease. The spraying of Acephate 75% SP @ 1.5 gm/lit+ Neem oil 0.15% EC @ 2.0 ml/lit. Spray of Imdacloprid 17.80% SL @ 0.5 ml/lit+ Neem oil 0.15% EC @ 2.0 ml/lit or spray of Admire @ 2 g/l+ Neem oil and Hostothion + Neem Oil at 10 days interval can be applied for management of the disease (YVMV). Likewise, development of virus resistant varieties has been considered the most effective, economical and resistant entries (2012/OK YVRES 5 and 2012/OKYVRES-6, 2012/OK YVRES 2) can be used for cultivation as well as for generating breeding material as well. Keywords: Disease incidence, Insecticides, Resistant entries

July 1-3, 2015

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Oral Abstracts

O-32

In Vitro Production of Cell Wall Degrading Enzymes by Pythium Species Isolated from Asymptomatic and Symptomatic Lettuce Root Chulalak TALUBNAK1, 2*, Nonglak PARINTHAWONG2 and Tanimnun JAENAKSORN2 1

Department of Biotechnology in Plant Pathology, International College, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok, Thailand 2 Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: chulalakkmitl@gmail.com

ABSTRACT This research investigated the ability of six isolates of Pythium species isolated from asymptomatic and symptomatic lettuce roots in producing cell wall degrading enzymes, eg. cellulase, pectinase and chitinase on solid media. The tested pathogens were grouped as the non to weakly virulent and the moderately virulent groups. Activities of cellulase and pectinase enzymes were determined by hydrolysis capacity (HC) value which is the ratio of the hydrolysis zone and colony diameter. For chitinase, its activity was categorized based on relative increase in diameter as well as intensity of purple color on colloidal chitin medium. The results revealed that all tested isolates of Pythium species were low potential to produce cellulase with HC value of less than 1.00 while absent cellulase was detected in Phytophthora IS40. Regard to pectinase enzyme production, absence as well as presence of activity with low HC value (<1.00) were observed in the non to weakly virulent group of Pythium. Meanwhile, only low HC value (<1.00) of pectinase was detected in moderately virulent group. For chitinolytic enzyme, two isolates in the non to weakly virulent group presented moderate activity and the rest showed absent activity. Keywords: Cellulase, Chitinase, Pectinase, Pythium

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Oral Abstracts

O-33

Gene Expression Analysis in Lettuce (Lactuca sativa L.) Treated with Trichoderma spp. Malatee PRADUBYAT1, 2*, Nonglak PARINTHAWONG1 and Tanimnun JAENAKSORN1 1

Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand *Corresponding email: mee_yard@msn.com

ABSTRACT This study was aimed at gene expression in hydroponically grown lettuce treated with 4 isolates of Trichoderma. The experiment was arranged into Completely Randomized Design having 9 treatments with 3 replications of 6 plants. Using polymerase chain reaction (PCR), expression of pathogenesis related protein (PR) 1 and low-affinity cation transporter (LCT1, LCT2) were analyzed from lettuce leaf of each treatment at 0, 24, 48 and 72 hrs after inoculation. Besides, plant growth as well as yield was recorded at harvest. Results revealed the good quality of total RNA extracts as the presence of 18s band were clearly observed. PR1 was observed while no LCT1 and LCT2 were detected. The expression level of PR1 gene was variable linked to the isolation of Trichoderma and incubation period. The tested Trichoderma had positive as well as negative effect but insignificant on yield of lettuce compared to control. Keywords: Trichoderma spp., Induced resistance, PR protein, Lettuce, Hydroponics

July 1-3, 2015

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Oral Abstracts

Assessment of Viability and Efficacy of Fusarium oxysporum (F221-B) as BCA and PGPF during Long Term Preservation Titi THONGKAMNGAM* and Tanimnun JAENAKSORN *Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding authors: Titi29950@hotmail.com

ABSTRACT Long term preservation of Fusarium oxysporum (F221-B) using modified filter paper technique was monthly assessed on the basis of its viability, morphological characteristics and in vitro efficacy as biocontrol agent (BCA) and plant growth promoting fungus (PGPF). The results showed that the 6month storage of F221-B was very satisfactory since its viability of cultures was still 100 percent until the end. Remarkable consistency in retaining its morphological characteristics was noted during preservation. Main hypha was in the range of 110-121x2.2-2.6 µm, hyphal tip was 82.04-94.04x3.2-3.5 µm whereas macro conidia were 28.23-29.85x3.0-3.5 µm. The biocontrol efficacy of F221-B against Colletotrichum sp., Curvularia sp. and Fusarium sp. remained relatively constant and unchanged during 6 months which was in the range of 36.1-74.8 percent from dual culture test and 31.6-48.2 percent from agar well diffusion assay when using 100 percent of culture filtrate concentration. Regarding its effectiveness as PGPF, the treated seeds with spore suspension of F221-B at 103 and 106 spores/ml could germinate 2-day faster than non-treated seeds resulting in higher percentage of seed germination and seedling length at a given day. To conclude, 6-month preservation of F221-B using modified filter paper technique was successful with 100 percent viability and its unchanged efficacy as BCA and PGPF. Keywords: Fusarium oxysporum (F221-B), Modified filter paper technique, Long term preservation, BCA, PGPF

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Oral Abstracts

O-36

Comparison of Trichoderma Population in the Re-Circulating Nutrient Solution With and Without Supporting Material Kanet JAIKENGKAJ1 , Tanimnun JAENAKSORN and Prommart KOOHAKAN* 1

ABSTRACT Effects of supporting material on Trichoderma population in the re-circulating nutrient solution were studied. The experiment was done in closed re-circulating system with or without supporting material. In the system with supporting material, vermiculite (1 L) mixed with 50 g of Trichoderma bioproduct was filled into the plastic bottle, while control was only bottle without supporting material. Nutrient solution passed through the container and ran off to the nutrient solution tank. Population of Trichoderma in the nutrient solution with and without supporting material was weekly detected. The result showed that Trichoderma population in the system with supporting material changed slightly by 4.4-5.3 log cfu/ml during seven weeks. Though in control, Trichoderma population reduced rapidly from 5.3 to 3.3 log cfu/ml within 2 weeks, then decreased to 2.6-2.7 log cfu/ml until the end of experiment. For Trichoderma population in the nutrient solution, it was lower than that with supporting material. Interestingly, the population in the nutrient solution with supporting material was 10 times higher than that of without supporting material. Keywords: Hydroponics, Trichoderma sp.

July 1-3, 2015

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Oral Abstracts

Morphological Identification of Trichoderma species from Different Habitats Suriyasit SOMNUEK1*, Pornprapa KONGTRAGOUL2 and Tanimnun JAENAKSORN1 1

Department of Plant Production Technology, Faculty of Agricul tural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand 2 Program in Horticulture, Division of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Prince of Chumphon Campus, Chumphon, Thailand *Corresponding email: suriyasitsom@gmail.com

ABSTRACT Trichoderma species were collected from four different habitats, eg. organic and non-organic rice field in Supanburi province, forest at Sakaerat Environment Research Station in Nakhon Ratchasima province and nutrient solution sample from hydroponic farm. Nine isolates were successfully isolated on the modified Trichoderma-selective media by soil plate and dilution plate techniques. The result of morphological identification showed all isolates grew completely on PDA (9 cm diameter) in 3 days at room temperature. The colony color was initially white and turned bright green to dark green later. Growth rate of colonies ranged between 2.68-4.07 cm/day. Mycelia were branched, septate, and hyaline. Single-celled light green, mostly globose conidia were formed and developed on phialide in the range of 2.6-3.5 ×2.5-2.9 µm. The phialides were in the range of 4.9-8.4 × 2.5-3.4 µm. These morphological characteristics were identical to the T. harzianum as reported in Chaveri and Samuels (2003). Keywords: Morphological identification, T. harzianum, Organic rice field, Non organic rice field

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Oral Abstracts

O-38

Resistant in Rice Cultivars against Sheath Blight Disease under Artificial Inoculated Conditions P.K. TIWARI1*, C.P.KHARE1 and A.S. KOTASTHANE1 1

Department of Plant Pathology, IGKV, Raipur-492012 (C.G.) India *Corresponding email: pradeep.tiwari95@gmail.com

ABSTRACT Sheath blight of rice caused by Rhizoctinia solani is one of the most serious diseases of irrigated rice. It is the major disease in particular Chhattisgarh state of India and Raipur district is considered as a hot spot location for the disease. In nature, the varieties sustained for sheath blight resistant are required and the process of selecting resistant varieties is continued. An, attempt therefore was made to identify the sources of resistance in available rice germplasm against the sheath blight disease. The two thousands and five hundred of rice cultivars were screened in different trails and a total number of 11 rice germplasms were collected on the basis of resistant reactions. The selected resistant germplams were re-screened by sowing in simple three row plots under artificial inoculated conditions during the consecutive seasons from 2011-2013. The seeds were sown in row of 2 m., spaced at 20X10 cm. in the field. The disease severity was recorded at 21 days after inoculation followed by SES scale (1996). Among the 11 cultivars tested against the sheath blight of rice under artificial inoculated conditions, none was found immune while 11 entries viz, IET22240, IET22250, IET21665, CBO9-153, Dhan Prasad, GP.no.545191, GP.no.461231, GP.no.545206, G.P no. 450284, GP no. 450296 & G.P.no 463893 proved to be resistant and locally adopted variety Swana produced highly susceptible reaction. Keywords: Sheath blight, Artificial inoculated condition, Resistant varieties, Entries, Disease severity

July 1-3, 2015

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Oral Abstracts

Influence of Weather Parameters on the Incidence of Okra Yellow Vein Mosaic Virus Disease and Whitefly (Bemisia tabaci) Population D.R. BHENDARKAR1*, C.P.KHARE1 and S.L. SWAMY1 1

Department of Plant Pathology, I.G.K.V., Raipur-492 006, Chhattisgarh, India *Corresponding email: bhendarkar.patho@rediffmail.com

ABSTRACT Okra (Abelmoschus esculentus (L.) Moench) is an important vegetable crop of India, grown in both rainy as well as summer seasons. One of major limiting factors for reduction of yield of okra is the incidence of yellow vein mosaic disease caused by Yellow vein mosaic virus (YVMV) and its vector whitefly (Bemisia tabaci). Weather parameters play a great role in the development of the whitefly population and the disease. Among the weather parameters, maximum and minimum temperature has strong negatively and significantly correlated with disease incidence. Whereas the weather parameters such as maximum and minimum temperature, relative humidity, wind speed and rainfall have the negative correlation with whitefly population per plant. Keywords: Yellow vein mosaic virus, Epidemic, Weather parameters, Vector whitefly, Disease incidence

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Oral Abstracts

O-41

A Crossbred High Yielding and Insect Resistant Cotton Variety Having Wider Climatic Adaptability Syed BILAL HUSSAIN1*, Zulfiqar ALI2 and Hasnain NAWAZ KHAN2,1 1

Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences & Technology, Bahaudin Zakariya University, Multan, Pakistan 2 Allahdin Group of Companies, 47-A, Industrial Estate, Multan, Pakistan *Corresponding email: syedbilalhussain@hotmail.com

ABSTRACT Allah Din-555 (A-555) has been bred by a cross of local cotton varieties (MNH-6070 X MNH-770) and exotic Bt. AGC-1085 (Bollgard-1). First cross was cracked in 2004 at Allah Din Research Centre, Jahanian. Selection procedure has been adapted up to sixth generations in a fashion of two crops per year using glass house. After F6 generation the line was bulked in 2007. All developmental tenured of A-555 completed before proliferation of National Bio-Safety rules, 2005. After completion of Micro varietal and Zonal varietal trials, A-555 examined two following years 2011-12 for different preliminary disease, yield, lint traits and confirmation of Bt. gene by DUS trials, Punjab Seed Corporation yield tests and National Coordinated Bt. Trials. In all trials, especially NCBT showed dominance in different characters. On the basis of all trials performance, A-555 was approved by the Pakistan Seed Council as a new insect resistant cotton variety for general cultivation in all cotton areas of Pakistan. The recommendation of sowing time of A-555 is the first week of May to mid June and 1st week of March for early sowing. Keywords: A-555, Bt. Cotton, CLCV

July 1-3, 2015

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Oral Abstracts

The Impact of Climate Variability on Agricultural Pest Ninio A. RELOX1* and Sharon Juliet M. ARRUEJO2 1

Philippine Atmospheric, Geophysical and Astronomical Services Administration Agham Road, Diliman, Quezon City, Philippines *Corresponding email: narelox@yahoo.com, s_arruejo@yahoo.com

ABSTRACT The impact of climate variability on insect pest occurrence was studied to establish information beneficial to agricultural pest management by monitoring the rates of pest invasion using light trap equipment and ascertain benchmarks of climate changes that influence distribution of the species of brown plant hopper, green leaf hopper, stem borer and parasitic wasp. Correlation and graphical analysis were employed to determine the significant relationship between the two years monthly totals of the pests and three climatic variables: temperature, rainfall and relative humidity. The result showed high correlation of brown plant hopper and green leaf hopper with temperature four months ago, (r2 = 0.630915 and 0.62171, respectively) and stem borer with temperature five months ago (r2= 0.7620). The result obtained for parasitic wasp, however, was highly correlated with rainfall next month (r2= 0.72762). Based on the facts presented, the conclusions drawn are as follows: (1) The impact of climate variability on insect pests occurrences are good inputs to agricultural pest management and farm weather forecasting; and (2) Parasitic wasp dynamics may be used to predict the rainfall for next month so that dam releases for irrigation may be efficiently scheduled. Keywords: Agriculture, Biometeorology, Climate, Insect, Pest

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Poster Abstracts

July 1-3, 2015

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Poster Abstracts

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Poster Abstracts

P-1

Effect of Naphthalene Acetic Acid and Thidiazuron on Phytotoxicity of Phenanthrene and Fluorene to Waxy Corn Waraporn CHOUYCHAI1*, Jaturaporn DONRODPRI1, and Khanitta SOMTRATOON2 1

Biology Programme, Faculty of Science and Technology, Nakhonsawan Rajabhat University, Nakhonsawan, Thailand 2 Department of Biology, Faculty of Science, Mahasarakham University, Mahasarakham, Thailand *Corresponding email: chouychai@yahoo.com

ABSTRACT Phenanthrene and fluorene are polycyclic aromatic hydrocarbons (PAHs) which are toxic to plant growth. This phytotoxicity is a limiting factor for a success of PAH phytoremediation. Thus, plant growth regulators were used to alleviate PAH toxicity. The effect of 2 plant growth regulators, naphthalene acetic acid (NAA) and thidiazuron (TDZ) to alleviate PAH phytotoxicity was studied. Waxy corn seeds were immersed in 10 mg/l NAA, or TDZ for 1 or 8 h, and then sown in 400 mg/kg phenanthrene and 400 mg/kg fluorene contaminated sand. Seeds immersed in distilled water for 1, and 8 h were done as control. Seedlings from NAA immersed seeds had higher root length and shoot fresh weight than control. On the other hand, seed immersion in TDZ decreased shoot length, root length and root fresh weight of waxy corn seedling compared to control. The immersion periods of 1 and 8 h affected only on root length and root dry weight of waxy corn seedling. For NAA, the 8 h immersed seed produced higher root length and root dried weight than that of 1h. This result indicated thatseed immersion in 10 mg/l NAA for 1 h could enhance waxy corn seedling growth in phenanthrene and fluorene-contaminated sand and will be useful for enhancing the plant growth in phytoremediation process. Keywords: NAA, TDZ, Polycyclic Aromatic Hydrocarbons, Waxy corn, Toxicity testing

July 1-3, 2015

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P-2

Poster Abstracts

Effect of Fertilizers on Physiological Traits Relate to Yield of Napier Pak Chong 1 Grass Nittaya PHAKAMAS1*, Anupong MAKLAI1, Tochchakorn PERMHIRUN1, Kittiya SERTSUNGNONE1 and Nawarat JAMPATHONG1 1

ABSTRACT Napier Pak Chong 1 (Pennisetum purpurem x Pennisetum amenicanum cv. Pak Chong 1) grass becomes one of the important economic forage crops in Thailand. The objective of this study was to determine the effect of chemical and organic fertilizers on the physiological traits relate to dry matter yield of Napier Pak Chong 1 grass. The experiment was conducted in the field of the Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, during 15 December 2013 to 18 December 2014. The experiment was laid out in a randomized complete block design (RCBD) with 4 replications. Plot size was 1 x 2 m, planted with spacing of 1.2 m between rows and 0.8 m between plants, using stem-cutting materials. Five types of fertilizer were used i.e., no fertilizer (control), cattle manure (12,500 kg ha-1), urea (93.75 kg ha-1), cattle manure (6,250 kg ha-1) + urea (46.87 kg ha-1) and 15-15-15 chemical fertilizer (312.5 kg ha-1). Plant height, tiller per plant, leaf area (LA), leaf area index (LAI), crop growth rate (CGR), chlorophyll A, chlorophyll B, fresh yield and dry matter yield were obtained for 4 times in every 60 daysinterval, and then analysis of variance and multiple regression were done for all those traits. The result found that, there was significant effect of fertilizers for chlorophyll A, chlorophyll B, LA, LAI, CGR, fresh yield and dry matter yield. The result indicated that CGR was the dominant physiological traits that contributed to yield of Napier Pak Chong 1 grass. Keywords: Forage crop, Energy crop, Physiological traits, Crop growth rate (CGR)

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Poster Abstracts

P-3

Effect of Nanoparticles on the Relationship between Crop Growth Rate and Yield of Chainat 1 Rice (Oryza sativa L.) Sutichai SAMART1*, Nittaya PHAKAMAS2 and Sutee CHUTIPAIJIT1 College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: samarteen_oxiy@hotmail.com 1

ABSTRACT Nanoparticle materials can be used in agriculture but knowledge is still limited. The objective of this study was to determine the effect of zinc oxide (ZnO) and titanium dioxide (TiO2) nanoparticles on physiological traits relate to rice yield. The experiment was conducted in the field of the Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang during February to June, 2013. Treatments were arranged in 2×5 factorials in RCBD with 3 replications. Zinc oxide and titanium dioxide nanoparticles were used as the factor A. Factor B was the concentrations of nanoparticles i.e., 100, 200, 400, 600 and 800 mgL-1. ZnO and TiO2, both types nanoparticle were used for every 14-days interval until 7 days before harvesting. Crop growth rate (CGR) was recorded at two stages; during planting to flowering (0 to 58 days) and flowering to harvest (58 days to harvest). Spikelet per m2, 1,000 grain weight, total dry matter, harvest index (HI) and grain yield were also collected. Analysis of variance and multiple regressions were done for all those traits. The results were found that there were significant effects of ZnO and TiO2 nanoparticles on growth and yield of Chainat 1 rice. TiO2 at a rate of 200-600 mg.L-1 promoted greater growth and yield of Chainat 1 rice than utilizing of ZnO. CGR was significantly correlated to yield of Chainat 1 rice (R2 =0.20*). Nanoparticles can promote CGR, especially, during flowering to harvest stage (58 days to harvest). Keywords: Chainat 1 rice, Physiological traits, Nanoparticle, Crop growth rate (CGR)

July 1-3, 2015

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Poster Abstracts

Antioxidant Determination of Nang Dam Upland Rice Bran Oil by DPPH Assay Vanapron SAE-ANG, Chitti TAWAI, Raumjit NOKKOUL and Duangkamol RUEN-NGAM1* Department of Biology, Faculty of Science, King Mongkut’s Institute of Technogy Ladkrabang, Bangkok 10520, Thailand *Corresponding email: krduangk@kmitl.ac.th, modeliebe@gmail.com

ABSTRACT Upland rice is one type of Thai rice grown on area in which ecosystem is different to Thai jasmine rice. The upland rice is grown on the dry land without flooding while only dew, rainfall and moisture in soil are enough to grow the upland rice. It can be grown in all parts of Thailand, especially in southern, Chumpron provinces is one of an important growing area. The upland rice normally has good smell and may be a source of nutrition such as protein and iron. Rice bran, by product from milling process, composes of many types of antioxidant such as vitamin E, -oryzanol, phenolics compounds and tocopherols. This research was carried out to evaluate the antioxidant activity of rice bran oil of the Nang Dam upland rice. Antioxidant activity was evaluated using the DPPH assay. DPPH radical inhibition percentage of the Nang Dam rice bran oil was compared to antioxidants standard such as BHT, Trolox and Ascorbic acid (Vitamin C), including with commercial rice bran oil which is available in the local market. Results found that DPPH radical inhibition of the Nang Dam rice bran oil is in the range of such upper standard. The research has been done on two range sizes; 150 and 850 m. The extraction process has been done by shaking method with circulation rate of 200 rpm in 30 minutes using ethanol as solvent in dark condition. Amount of the obtained Nang Dam rice bran oil extracted were 0.1010 and 0.0598 g/g dry weight for both range sizes of the rice bran and antioxidant activity for 50% DPPH radicals inhibition (IC50) at 4.14 mg/ml and 3.78 mg/ml for 850 and 150 m, respectively while the BHT, Trolox and Vit C IC50 were 0.1857, 0.0139 and 0.0110 mg/ml. However, IC50 of the commercial rice bran oil has less than 50% which has antioxidant inhibition around 34% at concentration of 10 mg/ml. This research has demonstrated the Nang Dam upland rice bran oil has potential to be an alternative antioxidant for consumers and entrepreneurs. Keywords: Rice bran oil, -oryzanol, % DPPH radical inhibition

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Poster Abstracts

P-5

Effect of Different Media and Concentrations of Growth Regulator on Callus Induction and Growing Suspension Cell Culture of San-pah-tawng 1 (Oryza sativa L.) Ranyikar PORAHA1, Anurug POEAIM1*, Saengthong PONGJAROENKIT2 and Pradit PONGTHONGKAM3 Department of Biology, Faculty of Science, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok, 10520, Thailand 2 Department of Genetics, Faculty of Science, Maejo University, Chiang Mai, 50290, Thailand 3 Thepstri Rajabhat University, 321 Naraimaharat Road Tambon Talaychubsorn Amphur Muang, Lopburi, 15000, Thailand *Corresponding email: kpanurug@kmitl.ac.th

ABSTRACT The aim of this study was to find out enhancement on callus induction and growing suspension cell culture in San-pah-tawng 1 rice. The mature seeds of rice were cultured on solid MS (Murashige and Skoog, 1962) and NB (Nitsch and Nitsch, 1969) media containing with 30 g/l sucrose combined with 1 g/l L-proline, 100 mg/l casein hydrolysate, 0.5, 1, 2, 3 and 5 mg/l 2,4D (2,4-Dichlorophenoxyacetic acid) and 2.6 g/l phytagel, Cultured in the dark condition for 4 weeks. NB medium was found better than MS medium for callus induction. The suitable media for callus induction of San-pahtawng 1 were NB medium supplemented with 2 mg/l 2,4-D, found to be effective for callus induction which induced the biggest size and the maximum weight of calli. After that, transferred the calli to liquid NB medium containing 1 mg/l 2,4-D, 30 g/l sucrose, 1 g/l proline and 100 mg/l casein hydrolysate. Study the growth phases at 0, 3, 6, 9, 12, 15, 18 and 21 days respectively, were determined by measuring fresh and dry weight of the cell. Keywords: San-pah-tawng 1, Callus induction, Cell suspension culture, Growth curve, 2,4-Dichlorophenoxyacetic acid

July 1-3, 2015

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Poster Abstracts

Elite Thein Corn Inbred Lines Utilized to be Synthetic Variety Kitti BOONLERTNIRUN1*, Suchada BOONLERTNIRUN1 and Choosak JOMPUK2 1

Department of Plant science, Faculty of Agricultural Technology and Agroindustry, Rajamangala University of Technology Suvarnabhumi, Phranakhon Si Ayutthaya, Thailand 2 Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand. *Corresponding email: kitti.b@rmutsb.ac.th

ABSTRACT Thein corn synthetic variety is alternative technology for farmers who need to improve their Thein corn production to be high yield and quality. Good synthetic variety was actually produced from good general combining ability (GCA) of inbred parents. In this study, inbred lines were extracted by standard method from three open pollinated Thein corn populations and 4 hybrid populations were also extracted. Such S4 inbred lines were selected to be 101 lines and then crossed with 3 testers, TBK-17-1-1-1 (T1), TKKU1-72-1-1 (T2), and TSW-23-2-1-1-1 (T3), to produce 259 top crosses. Yield trials were done using augmented design. Line x tester was analyzed and GCA in terms of yield was also evaluated and found that top cross line of tester named T1, T2 and T3 had averaged ear numbers 95,188, 100,950 and 102,388 ears/ha respectively. Dehusked yield of such lines was 7.63, 7.25 and 7.75 ton.ha-1 respectively. Ten selected prolific S 4 inbred lines were L3, L5, L22, L23, L28, L29, L47, L51, L59 and L65. The ear numbers of such selected lines ranged 112,225 to 124,444 ears.ha-1 and GCA value in terms of ear numbers was between 12,494 to 24,719 ears.ha-1. Key words: Thien corn, Synthetic variety, Inbred line

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Poster Abstracts

P-7

Hypoxic Responses of 6 Commercial Waxy Corn Varieties Suchada BOONLERTNIRUN1* Raweewun SUVANNASARA1 and Kitti BOONLERTNIRUN 1 1

Faculty of Agricultural Technology and Agro-industry, Rajamangala University of Technology Suvarnabhumi, Phranakhon Si Ayutthaya province, Thailand 13000 *Corresponding email: suchada.b@rmutsb.ac.th

ABSTRACT Hypoxic soil condition is defined as oxygen deficiency in soil which negatively affects growth and yield potential of many crop species. The objective was to evaluate hypoxic tolerance at V7 growth stage of 6 commercial waxy corn varieties. Pot experiment was performed using a split plot in Randomized complete block (RCBD) with 4 replications. Hypoxic and normal conditions were used as main plot whereas waxy corn varieties named Big white 852, Sweet white 25, Violet white 926, Neaw simoung 111, Tender 58 and Neaw wan 4985 were used as subplot. The results indicated that there was a significant difference between normal and hypoxic conditions on leaf greenness, plant height, leaf area, dry weight and relative growth rate of all waxy corn varieties, negative effect was remarkably observed under hypoxic condition. Considering variety effect, it indicated that each waxy corn variety differently responded to hypoxia. Big white 852 and Sweet white 25 varieties showed high waterlogging index on dry weight, leaf greenness, plant height and leaf area, this may be explained that these two varieties tended to well adapt under hypoxic condition. Keywords: Hypoxic condition, Waxy corn, Waterlogging index

July 1-3, 2015

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Poster Abstracts

P-8

Development of Chitosan-Based Silver Nanoparticles Coating and Study of Its Effect on Litchi Stored at Ambient Temperature Warin PIMPA1* and Chakkrit PIMPA2 1

Department of Agro-Industry, Faculty of Agriculture, Natural Resource and Environment, Naresuan University, Phitsanulok 65000, Thailand 2 Northern Region Operation Division, Electricity Generating Authority of Thailand, Phitsanulok 65000, Thailand *Corresponding email: warin@nu.ac.th

ABSTRACT The fabrication of silver (Ag) nanoparticles was accomplished by reducing silver nitrate with nontoxic and biodegradable chitosan. The Ag nanoparticles presented a spherical shape with a wide size range of 10-25 nm, as observed by transmission electron microscope (TEM). Ag nanoparticles-loaded chitosan coatings were formed from the starting materials composed of Ag nanoparticles and chitosan via thermal treatment. Compared with pure chitosan coatings, Ag nanoparticles-loaded chitosan coatings showed both fast and long-lasting antibacterial effectiveness against both Gram-positive and Gram-negative bacteria. The results suggested that Ag nanoparticles-loaded chitosan coatings could be feasibly as antimicrobial materials for food applications. In this study, the effects of Ag nanoparticlesloaded chitosan coatings on disease incidence and qualities of litchi fruit at ambient temperature were evaluated. The application of Ag nanoparticlesloaded chitosan coatings delayed the decrease in anthocyanin content, the increase in PPO activity and the changes in colour index. The application also reduced the decrease in concentrations of total soluble solids and titratable acidity and effectively inhibited decay. Hence, it could be concluded that treatment with Ag nanoparticles-loaded chitosan coatings exhibited a potential for shelf life extension of litchi at ambient temperature. Keywords: Silver nanoparticles, Chitosan, Litchi, Shelf life

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Poster Abstracts

P-9

Green Synthesis of Silver Nanoparticles Loaded onto Activated Carbon Using Banana Peel Extract for Environment Applications Warin PIMPA1* and Chakkrit PIMPA2 1

Department of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand 2 Northern Region Operation Division, Electricity Generating Authority of Thailand, Phitsanulok 65000, Thailand *Corresponding email: warin@nu.ac.th

ABSTRACT Silver (Ag) nanoparticles were successfully synthesized from aqueous silver nitrate through a simple green route using the extract of banana peel as a reducing as well as capping agent. The results obtained from UV-visible spectroscopy and transmission electron microscope (TEM) support the biosynthesis and characterization of Ag nanoparticles. Ag nanoparticles are in size range of 10-30 nm. Furthermore, the antibacterial application of these biological synthesized Ag nanoparticles loaded onto activated carbon has also been investigated. The presence of Ag nanoparticles does not change properties of the activated carbon in terms of morphology, examined by scanning electron microscope (SEM) and methylene blue adsorption ability. The Ag nanoparticles-loaded carbon shows a good antibacterial activity against Escherichia coli with very low minimal inhibitory concentration of 15 ď g/ml. The materials are potential for prevention and treatment of microbial infection and contamination for environmental application. Keywords: Silver nanoparticles, Banana peel, Activated carbon

July 1-3, 2015

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Poster Abstracts

Bioactive Compounds and Antioxidant Activities in Flesh, Leaves and Endocarp of Carissa carandas Compared to Grape Seed Extract Products Surasak SAJJABUT1*, Wachiraporn PEWLONG1, Sirilak CHOOKAEW1, Jaruratana EAMSIRI1 and Panchalee PRAKHONGSIL1 1

Thailand Institute of Nuclear Technology (Public Organization), Ongkharuk, Nakhon Nayok 26120, Thailand *Corresponding author: saksajja@yahoo.com

ABSTRACT Carissa carandas (Nhamdang in Thai) is an herbal plant commonly found in Thailand. The purposes of this study were to analyse for total phenolics, free radical scavenging power (DPPH assay), ferricâ&#x20AC;&#x201C;ion reducing power (FRAP) and proanthocyanidins (vanillin-HCL assay) in flesh, endocarp and leaves of C. carandas comparing with two brands of grape seed extract products (BM and VT). The results showed that the total phenolics and free radical scavenging power in grape seed extract products (BM and VT) were higher than those in C. carandas samples, while the highest FRAP value was found in the endocarp and BM product. Surprisingly, this study indicated that proanthocyanidin content (an important bioactive compound in grape seeds) in C. carandas endocarp was higher than in BM and VT grape seed products. Keywords: Carissa carandas, Grape seed extract, Antioxidant, Proanthocyanidins

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Poster Abstracts

P-11

Gamma Radiation Effects on Anthocyanins, Vitamin C Content and Antioxidant Activity of Carissa carandas Wachiraporn PEWLONG1*, Surasak SAJJABUT1, Jaruratana EAMSIRI1, Sirilak CHOOKAEW1 and Panchalee PRAKHONGSIL1 a

Thailand Institute of Nuclear Technology (Public Organization), Ongkharuk, Nakhon Nayok 26120, Thailand * Corresponding email: wachiraporn03@yahoo.com

ABSTRACT Food and agricultural products can be preserved for long term storage by gamma radiation. A low dose of gamma radiation has been reported to improve the antioxidant activity of some fruit extracts. The aim of this study was to investigate the effect of gamma radiation on antioxidant activity, anthocyanins and vitamin C content in Carissa carandas which is commonly known as nham-dang in Thailand. Ripe fruit samples were irradiated with gamma rays for total doses of 0.3 to 2.0 kGy at a dose rate of 5.2 kGy/h. Antioxidant activities of fruit extracts were evaluated based on total phenolic content, 1;1-diphenyl-2-picryhydrazyl (DPPH), free radical scavenging ability, and ferric-ion reducing power (FRAP). In addition, total anthocyanin content was analyzed by pH differential method, and vitamin C content was determined by High Performance Liquid Chromatography (HPLC). Results showed that radiation treatment up to 2.0 kGy did not affect the level of anthocyanin and vitamin C contents. In terms of antioxidant activities, there were negligible changes in the amounts of total phenolic and total anthocyanin content in fruit extracts following irradiation. No significant differences in the antioxidant activity and vitamin C content were observed between non irradiated and irradiated samples. These results indicated that gamma radiation up to 2.0 kGy did not affect the chemical properties of C. carandas. Keywords: Gamma radiation, Carissa carandas, Antioxidant

July 1-3, 2015

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P-12

Poster Abstracts

Influence of Electron Beam Irradiation on Hygienic Quality and Antioxidant Activities of Ground Sea Holly (Acanthus ebracteatus) Jaruratana EAMSIRI 1*, Surasak SAJJABUT1, Wachiraporn PEWLONG1 and Sirilak CHOOKAEW1 1

Thailand Institute Nuclear and Technology (Public Organization), Ongkharak, NakornNayok, 26120, Thailand *Corresponding email: jarurattt@gmail.com

ABSTRACT Roles of electron beam irradiation on improving hygienic quality and antioxidant activities of groundsea holly (Acanthus ebracteatus) were studied. The radiation processing was carried out at the doses of 0, 5, 10, 15 and 20 kGy using an electron beam accelerator (Mevex, MB 20/16) at the energy of 8 MeV, beam current of 10 mA and dose rate of 5 kGy/pass. The irradiated and unirradiated samples were analyzed for microbiological aspects and antioxidant properties. Results indicated that irradiation using electron beam significantly reduced microbial contamination. After irradiation at 5 kGy, total viable bacterial count (TVC) and total yeast and mold (TYM) were diminished by 2 and 1 log cycle, respectively. The dose of 5 kGy was adequate to diminish TVC and TYM to meet the Thai Community Product Standard of dry herbs (TCPS.480/2557) and to purge of pathogens such as coliform bacteria, Escherichia coli, Salmonella spp. and Clostridium perfringens. The antioxidant properties such as total phenolic content (TPC), ferric reducing antioxidant potential (FRAP) and free radical scavenging activity (DPPH) displayed insignificant changes for all doses applied in this study. Keywords: Sea holly, Electron beam, Irradiation, Microbiological quality, Antioxidant activity

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Poster Abstracts

P-13

Effect of Alginate Edible Coating on Quality of Fresh-cut ‘Kimju’ Guava A. ONDEE1* and S. MANURAKCHINAKORN1 1

Walailak University, Nakhon Si Thammarat, 80161, Thailand *Corresponding email: msuprane@wu.ac.th

ABSTRACT Fresh-cut fruits have always been popular with consumers because of wonderful flavor and freshness, along with added convenience. Guava is a climacteric fruit which is highly perishable and undergoes rapid postharvest changes. Physical damages during minimal processing increase metabolic activities leading to accelerated quality deterioration of fresh-cut guava during storage. Edible coating has been reported to offer a possibility to extend shelf life of several fresh-cut fruits. Therefore, this research aimed to investigate some important quality changes of fresh-cut ‘Kimju’ guava during storage as affected by alginate edible coating. Guava was cut into 6 wedges before coating with 1.5 and 2% alginate. Fresh-cut guava without coating was served as a control. The fruit was then put in polystyrene trays, prior to packaging in sealed polypropylene bags and storage at 10oC for 8 days. After storage for 4 days, there was no difference in total color difference (∆E*) between fruit coated with 1.5% alginate and the control (p>0.05), however coating with 2% alginate enhanced ∆E* of the fruit (p<0.05). At the end of storage, fruit coated with 1.5% alginate exhibited the lowest ∆E*, compared with other treatments (p<0.05). Total soluble solids of fruit coated with 2% alginate was lowest throughout the storage (p<0.05), followed by 1.5% alginate and the control, respectively. Firmness of 1.5 and 2% alginate treatments were higher than the control on day 8 (p<0.05), though there was no difference between the treatments (p>0.05). Coating with 1.5% alginate had potential to delay discoloration and ripening of freshcut guava. Keywords: Alginate, Edible coating, Fresh-cut guava, Quality, Storage

July 1-3, 2015

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P-14

Poster Abstracts

Evaluation of Antioxidant Activities of Essential Oils from Peppermint and Ginger Natthakiti PHURUEN1, Chamroon LAOSINWATTANA1 1* and Montinee TEERARAK 1

ABSTRACT The aim of this study was to evaluate antioxidant activities of essential oils of peppermint (Mentha piperita L.) and ginger (Zingiber officinale Roscoe) by using DPPH (2, 2-diphenyl-1-picrylhdrazyl) free radical-scavenging, metal chelating and reducing power activities. Both essential oils exhibited a radical scavenging activity against DPPH radical with IC50 (half maximal inhibitory concentration) values of 7,870.56 and 93,005.75 mg/L for ginger and peppermint essential oils, respectively. The IC50 values of standard antioxidants were 3.16 mg/L for ascorbic acid and 21.82 mg/L for butylated hydroxyl toluene (BHT). Furthermore, the essential oils also exhibited metal chelating activity with IC50 365.68 mg/L for ginger essential oil and 989.13 mg/L for peppermint essential oil while ethylene-dinitrilo-tetraacetic acid (EDTA) as standard IC50 value was 18.32 mg/L. Moreover, reducing power ability of essential oils of peppermint and ginger increased concentrationdependently and the ability was low in essential oil of peppermint compared with essential oil of ginger. Keywords: Antioxidant activity, Essential oil, Metal chelator, Reducing power

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Poster Abstracts

P-15

Improved Efficiency of Anthocyanin Extraction from Roselle Using Acidified Solvent F. NUUDOM1* and S. MANURAKCHINAKORN1 1

Walailak University, Thasala, Nakhon Si Thammarat, 80161, Thailand *Corresponding email: msuprane@wu.ac.th

ABSTRACT Anthocyanin is an important pigment in nature which is responsible for the shiny orange, pink, red, violet and blue colors in flowers and fruits of some plants. Utilization of anthocyanin as a natural food colorant has recently received increasing attention. Anthocyanin possesses a wide range of biological activities including antioxidant activity, which plays a vital role in prevention of many plant diseases. Among various plant materials, roselle calyx is recognized as a good source for anthocyanin extraction. Solvent extraction has been the most common method for anthocyanin extraction from several plant sources. This study aimed to investigate the effect of nontoxic solvents on anthocyanin extraction from roselle. Dried roselle calyx was extracted with water and ethanol, with and without 0.3% citric acid, for 1 h at 25oC using 1/25 of solid-to-solvent ratio. After filtration and centrifugation, the extract was subjected to property analysis. The highest anthocyanin content and antioxidant capacity (p<0.05) were detected in the extract using acidified water extraction, followed by water, acidified ethanol and ethanol extraction, respectively. Higher content of total phenolic compounds (p<0.05) was obtained using water extraction, compared with ethanol extraction. On the other hand, the extract using ethanol extraction showed higher a* and lower L* (p<0.05), compared with water extraction. Addition of citric acid did not affect the total phenolic compounds content, a* and L* (p>0.05) of the extracts of both water and ethanol extraction. The results revealed that acidified water was the most effective solvent for extraction of anthocyanin and antioxidant from roselle. Keywords: Anthocyanin, Citric acid, Extraction, Roselle, Solvent

July 1-3, 2015

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P-16

Poster Abstracts

Antioxidant Activity and Total Phenolic Contents of Tagetes erecta L. Leaves Extracts Pattharin WICHITTRAKARN, Montinee TEERARAK and Chamroon LAOSINWATTANA* Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: klchamro@kmitl.ac.th

ABSTRACT This study was performed to evaluate the antioxidant potential of the flower extract from Tagetes erecta L. The ethanol is a recommended solvent for extracting antioxidants from this plant. The antioxidant activity was evaluated using 2, 2-diphenyl-l-picrylhydrazyl (DPPH) radical scavenging activity, metal chelating ability and total phenolic content. The result showed that DPPH free radical-scavenging activity with the half maximal inhibitory concentration (IC50) value was 111.84 ppm compared with IC50 values of standard antioxidants were 3.16 ppm for ascorbic acid and 21.82 ppm for butylated hydroxyl toluene (BHT), respectively. In addition, the flower extract has metal chelating activity with IC50 with of 22490.11 ppm and ethylene-diaminetetraacetic acid (EDTA) was used as standard of 18.32 ppm. Furthermore, the phenolic content was found to contain 4203.455 mg GAE/100g of dry weight (expressed as gallic acid equivalents). The results indicate that these compounds contribute to the antioxidative activity. Keywords: Tagetes erecta L., DPPH free radical-scavenging, Metal chelating ability, Total Phenolic content

Page 106

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Poster Abstracts

P-17

Allelopathic Effect of Fresh and Dried Leaves Aqueous Extracts of Marachra capitata L. and the Action of Allelochemicals in Different Soil Types Phawinee KAMSAN, Pattharin WICHITTRAKARN, Montinee TEERARAK, and Chamroon LAOSINWATTANA* Department of Plant Production Technology, Faculty of Agricultural and Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand

*Corresponding email: klchamro@kmitl.ac.th ABSTRACT Allelopathic effects of aqueous extracts from fresh and dry leaves of Marachra capitata L. at concentrations of 1.25, 2.5, 5 and 10% were evaluated on germination and seedling growth of barnyard grass (Echinochloa crus-galli (L.) Beauv.) and wild pea (Phaseolus lathyroides L.). The distilled water was used as the control. It was found that aqueous extract from dry leaves had inhibitory effect on seed germination and seedling growth of barnyard grass and wild pea was stronger than aqueous extracts from fresh leaves. In addition, the concentration of 5% aqueous extract from dry leaves had completely inhibited seed germination of wild pea. The influence of 5 various planting media (fertile soil, fertile sand and sterile soil, sterile sand and only leaves powder) on dry leaf potential was also studied at concentrations of 250 and 500 mg/ petri-dish on germination and seedling growth of barnyard grass and wild pea compared with only germination paper (control). The results indicated that at 500 mg/ petri-dish had completely inhibited on seed germination and seedling growth of wild pea. Keywords: Marachra capitata L, Germination and seedling growth, Fresh and dry leaves

July 1-3, 2015

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Poster Abstracts

P-18

Effects of Natural Herbicide from Piper betle Linn. on Seed Germination, Imbibition and α- Amylase Activity of Amaranthus gracilis desf. Pariyaporn NETSAWANG1, Pattharin WICHITTRAKARN1, Montinee TEERARAK1 and Chamroon LAOSINWATTANA1* 1

Department of Plant Production Technology, Faculty of Agricultural and Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: klchamro@kmitl.ac.th

ABSTRACT The study on natural product herbicide from Piper betle Linn in soluble liquid (SL) formula at concentrations of 312 625 1,250 and 2,500 ppm on seed germination seed imbition and α- amylase activities of Amaranthus gracilis Desf were evaluated. The distilled water used as the control. The results showed that at 2,500 ppm completely inhibited seed germination of A. gracilis. The repeated experiment was conducted at concentrations 2,500 ppm on seed imbibition and α-amylase activities of A. gracilis. The results showed that seed germination of A. gracilis were decreased, while increasing of the concentration. These results indicated that the inhibitory effects of natural herbicide on seed germination of A. gracilis might be resulted from inhibiting seed imbibition and decreasing of α-amylase activities of A. gracilis. Keywords: Natural herbicide, P. betle, A. gracilis, α-amylase, Imbibition

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Poster Abstracts

P-19

Effect of different temperatures on carotenoid content and antioxidant activity in ‘Khak Dam’papaya Kanthee SIRIVEJABANDHU1 and Lampan KHURNPOON 1* 1

Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: kplampan@gmail.com

ABSTRACT The effect of different temperatures (4, 12 and 25°C) on carotenoid content and antioxidant activity in whole fruits and fresh cut ‘Khak Dam’ papaya fruits were studied. At 6 days in storage of whole fruits at 4 and 12°C showed significant difference in L*, a* and b* values in peel and pulp color from fruits stored at 25°C. Fruits stored at 4 and 12°C had lower in a* value of the peel about -10.1 and -8.6 respectively while it was about 3.3 when stored at 25°C. Low temperature storage could delay the reduction in fruit firmness, about 126.4, 98.9 and 66.1 N after being stored at 4, 12 and 25°C, respectively but lower in percentage of weight loss and TSS/TA ratio. The percentage of free radical inhibition in fruit stored at 4, 12 and 25°C were about 62, 64 and 70%, respectively. Highest carotenoids content was found when fruit stored at 4°C about 3.4 µg/g FW. In fresh cut sample after being stored at 4 and 12°C showed significant difference in L* and a* values of the pulp but no difference in b* value when compared to sample stored at 25°C. Fruits stored at 4 and 12°C could delay the change in a* value of the pulp and preserved the fruit firmness better than fruit stored at 25°C. Significant difference between storage temperatures was found in percentage of weight loss but not in TSS/TA ratio. Low temperature storage could maintain the change in carotenoid content but lower in the percentage of free radical inhibition than stored at 25°C. Keywords: Papaya, weight Loss, Free radical, Carotenoids, Antioxidant

July 1-3, 2015

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Poster Abstracts

P-20

Nitric Oxide Improves Cowpea (Vigna unguiculata [L.] Walp.) Growth Under Lead Stress Omid SADEGHIPOUR* Department of Agronomy, Yadegar-e-Imam Khomeini (RAH) Branch, Islamic Azad University, Tehran, Iran * Corresponding author: osadeghipour@yahoo.com

ABSTRACT Lead (Pb) is one of the hazardous heavy metals which its increasing levels in soil exerting a wide range of adverse effects on growth and metabolism of plants. Nitric oxide (NO) is an important signal molecule modulating responses to both biotic and abiotic stresses in plants. In the present study, the effect of exogenous NO on some traits of cowpea plants exposed to 0 and 200 mg Pb (NO3)2 kg-1 soil was investigated. Seeds soaked for 20 h with 0.5 and 1 mM sodium nitroprusside (SNP) as NO donor. Results showed that Pb toxicity significantly reduced plant height (35%), seed protein content (21%) and protein yield (65%) as compared to control. On the other hand, exogenous NO improved measured traits under lead stress. Both NO levels were effective in Pb stress tolerance although 0.5 mM was more effective. These results indicate that NO plays an important role in protecting cowpea plants against Pb toxicity. Keywords: Pb toxicity, Plant height, Protein yield, Seed protein content

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Poster Abstracts

P-21

Nutrient Dynamics in an Aquaponic System Somsak MANEEPONG* School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, Thailand *Corresponding email: msomsak@wu.ac.th

ABSTRACT Aquaponics is integrated system of circulating aquaculture andhydroponics. Principal objectives of the systems are reducing waste discharge and maximizing nutrient use. An aquaponic system comprised of a 500 l fish tank, a sedimentation and pH control tank, a degassing tank and three vegetable growing beds. It was assembled and tested for 17 weeks. Fifty Nile Tilapia (Oreochromis niloticus) were reared and daily fed three times with a complete diet containing 31.8% protein. Dead corals were installed for pH controlling, and maintaining for having vegetables at different growth stages in the system throughout the experiment. Water convolvulus (Ipomoea aquatica) and Tokyo Bekana (Brassica rapa) were rotationally grown. Water samples were taken once a week for analysis of pH, NH3/NH4+, NO2-/NO3-, H2PO4-, SO42-, K, Ca, Mg and Fe. Fish weight increased from 50 g/fish at the beginning to 228 g/fish at 15 weeks. Water pH increased from 6.0 before rearing to 7.0 at the 4th week, and varied within a small range of 6.9 - 7.0 until the end of experiment without any additional acid or alkali. Total NH3/NH4+ increased to 10.2 mg-N/L at the 2nd week, and rapidly declined to lower than 2.0 mg-N/L. H2PO4-, SO42- and Mg accumulated in the system, whereas Ca gradually increased and reached equilibrium at 47ď&#x201A;ą2 mg/l. K and NO2-/NO3- varied greatly at low concentrations comparison to general requirement of vegetables. Both of the vegetables grew well at the first crops, but their growth rates declined greatly and showed complicated nutrient deficiency. Keywords: Aquaponics, Nile Tilapia, Plant nutrient

July 1-3, 2015

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P-22

The Effects of Calcium Silicate on Density of Trichomes in Field Corn Pongsakorn NITMEE1, Pornpairin RUNGCHAROENTHONG1,* Suphachai AMKHA2 and Thongchai MALA2 1

Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, KamphaengSaen Campus, Nakorn pathom 73140, Thailand 2 Department of Soil Science, Faculty of Agriculture, Kasetsart University, KamphaengSaen Campus Nakorn pathom 73140, Thailand *Corresponding email: faasprr@ku.ac.th

ABSARACT The effects of Ca2SiO4 were studied on density of trichome in field corn at Kasetsart University, KamphaengSaen Campus from March to November in 2014. Experimental design was 2x7 fatorials in CRD with factor 1) noncoated seedand Ca2SiO4coated seeds,factor 2) application rates of Ca2SiO4 in soil 0, 31.25, 37.50, 43.75, 50, 56.25 and 62.50 kg/ha. The results showed that Ca2SiO4induced the density of trichome in leaves of field corn. Seeds coated with Ca2SiO4 gave the higher the number of trichomes(2.25trichome/mm) than non-coated (0.75trichome /mm). Both coated and non coated seeds combined with Ca2SiO4 31.25-62.5 kg/ha in soil increased the number trichome 2-3 time higher than the control.The higher the rate of Ca2SiO4 into soil, the larger the number of trichome was. Moreover, coated seeds and combined withCa2SiO4 in soil increased more number trichome than non-coated seed. In addition, field corn treated with Ca2SiO4 increased in leaf thickness which was higher than the control. Keywords: Calcium silicate, Field corn, Trichome

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P-23

Utilization of Zeolite and Isolite as Soil Conditioner to Improve Sandy Soil Nukoon TAWINTEUNG1* 1

Department of Plant Production Technology, Faculty of Agriculture Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: ktnukoon@kmitl.ac.th

ABSTRACT Utilization of zeolite and isolite as soil conditioner was carried out in order to improve sandy soil (Ng Series). The experiment was laid out in Completely Randomized Design (CRD) consisted of 8 treatments with 4 replications i.e. 5 and 10% (w/w) of zeolite, isolite and isolite cake, compost (2 ton/rai) as a compared treatment and chemical fertilizer as a control. The soils were collected in 3 layers (0-15, 15-50 and 50-100 cm) and 75 kg of soils were packed into each lysimeter (PVC column with 24.5 cm diameter and 120 cm long) in optimum bulk density and soil layers. All treatments were received N, P2O5 and K2O 112.5, 112.5 and 56.25 kg/ha, respectively and a sweet corn was planted in each lysimeter. Plant growth and yield were recorded. Plant samples were analyzed for nutrients concentration. Leachates were collected and analyzed for nutrients leaching. The results of this study indicated that CEC of zeolite was very high (102 cmol kg-1) resulted to an increase of CEC of sandy soil to 18.2 cmol kg-1 for 10% application. Compost and isolite cake were very high of organic matter and available phosphorus. The applications of soil conditioners affected to growth and yield of corn. The highest of fresh ear yield and nutrients uptake (macro nutrients) per lysimeter was found in zeolite 10% followed by zeolite 5% and compost, respectively. It was found only the trend that zeolite application could decrease nitrate and ammonium leaching as compared to control. After cropping, isolite cake increased soil pH from 4.75-4.92 to 7.51-7.57 whereas available phosphorus was increased to 57.1-65.0 mg kg-1.Soil organic matter was decreased in all treatments. CEC of 10% zeolite was decreased to 11.8 cmol kg-1. Keywords: Soil conditioner, Sandy soil, Zeolite, Isolite

July 1-3, 2015

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Poster Abstracts

Dendoremediation Efficiency of Forest Hardwoods to Remove Cadmium Toxicity from Contaminated Soil Syed AMIR MANZOOR1, Din MUHAMMAD ZAHID KHAN1, Muhammad ZUBAIR1, Wasif NOUMAN1, Sarwat NAZ MIRZA2, Syed BILAL HUSSAIN3, Feehan HASSAN3 and Muhammad IMRAN UMAR3 1

Department of Forestry &Range Management, FAS&T, Bahauddin Zakariya University Multan, Pakistan 2 Department of Plant Breeding &Genetics, FAS&T, Bahauddin Zakariya University Multan, Pakistan 3 Department of Forestry &Range Management, PMAS-Arid Agriculture University Rawalpindi, Pakistan *Corresponding email: amir.kzd@gmail.com

ABSTRACT Use of tree species to sequester heavy metal contamination in soil is an emerging and promising technique to treat the widespread marginal lands across the globe. Dendroremediation is not only a cost effective and eco-friendly option to remove metal toxicity from soil but it is also the only viable solution to permanently dispose of the problematic metals which have turned fertile lands into unproductive ones. This study aimed at evaluating the comparative efficiency of different tree species growing in the tropical arid environment of the Southern Punjab, Pakistan to accumulate Cadmium metal from contaminated soil. One year old seedlings of tree species namely Acacia nilotica, Albizia lebbeck, Eucalyptus camaldulensis, Dalbergia sisso, Azadirachta indica and Melia azedarach were used in this experiment to sequester cadmium from contaminated soil in a pot experiment. Five different concentrations of cadmium toxicity were used as treatments (10, 20, 30, 40, 50 ppm) and cadmium free pot soil was used as control. Growth performance and physiological response of tree species to metal stress was observed through parameters like changes in fresh and dry biomass of seedlings, plant height, chlorophyll content, accumulation of cadmium in plant parts (roots, stems, leaves) and removal of cadmium content from pot soil. Results showed that Acacia nilotica and Eucalyptus camaldulensis are the most useful tree species for dendroremediation of cadmium contaminated soils since these tree species showed minimum decrease in morphological and physiological growth and sequestered maximum cadmium ions from pot soils. Keywords: Cadmium, Dendroremediation, Metal toxicity, Phytoremediation.

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P-25

Leaf Flushing Inhibition by Application of High Phosphorus and Potassium Fertilizers for Off-season Mango Production Kannikar KAEWSONGSANG1*, Apisak BAOLEE1 and Ravie SETHPAKDEE2 1

Business Research and Development Division, SOTUS International CO., LTD., Pak Kret, Nonthaburi, Thailand 2 Department of Horticulture, Kasetsart University Nakhon Pathom, Thailand *Corresponding email: kannikar@sotus.co.th

ABSTRACT A key obstacle in off-season mango production is early leaf flushing. Although application of foliar 0-52-34 fertilizer can inhibit flushing. Consecutive sprays can lead to a difficulty in the flower induction. This research aims to investigate how new foliar 0-40-22 fertilizer inhibit leaf flushing and affect the subsequent flowering. The experiment was conducted at Sung Neon district, Nakhon Ratchasima province and was composed of no foliar fertilizer (control), 0-52-34 at 5g/L, 0-40-22 at 2.5 g/L and 5.0 g/L fertilizers. The foliar fertilizer applications commenced at 49 days after pruning at leaf pre-mature stage and two more re-applications were made at weekly intervals. Flowering of mature shoots were induced by thiourea and potassium nitrate at 70 or 90 days after pruning. The shoots were sprayed with 0-52-34 at 5 g/L, 0-40-22 at 2.5 g/L and 5.0 g/L fertilizers flushing at 28, 45 and 25% notably lower than the control flushing 100%. For induced flowering at 70 days after pruning, the shoots treated with 0-52-34 at 5 g/L, 0-40-22 at 2.5 g/L and 5.0 g/L fertilizers flowered 73, 45 and 81%, respectively, whereas there were no flowering on the control shoots. Similarly, the shoots induced at 90 days after pruning flowered at 71, 65 and 69%, respectively. The inflorescences from the shoots treated with 0-52-34 at 5 g/L, 0-40-22 at 2.5 g/L and 5.0 g/L fertilizers were 42.0, 49.2 and 49.2 cm in length, existing 22.8, 24.2, 27.7 peduncles, respectively. The results indicated that foliar application of 0-40-22 at 2.5 or 5.0 g/L can appreciably inhibit leaf flushing, resulting in simple flower induction and healthy inflorescences afterwards. Keywords: Flowering, Monopotassium phosphate , NUTAC速 Extra-P, Potassium nitrate, Thiourea

July 1-3, 2015

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P-26

Sand-Size Distributions of Soils on the So-Called Aeolian Sand Splay Landform in the Lower Mun-Chi Basin, Northeast Thailand Pornthiwa KANYAWONGHA1 and Anongnat SRIPRACHOTE2 1

Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut 's Institute of Technology, Ladkrabang, Bangkok 10520, Thailand 2 Department of Plant Science and Natural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand *Corresponding email: kkpornth@kmitl.ac.th

ABSTRACT This study aimed at determining the sand-size distribution of soils on the so-called aeolian sand splay landform located on the right side of the Lam Se Bai, the Chi, Lam Sieo Yai and the Mun rivers, northeast Thailand. Seven pedons were collected. After particle size analysis, the sand particles were separated into 5 sizes: the very coarse sand (VCS: 2.00-1.00 mm), the coarse sand (CS: 1.00-0.50 mm), the medium sand (MS: 0.50-0.25 mm), the fine sand (FS: 0.25-0.106 mm) and the very fine sand (VFS: 0.1060.053 mm). The soils had similar morphology, which homogeneous coarse textures throughout the depth, brownish, reddish and yellowish colors. Their sand contents ranged from 60% to more than 90% whilst approximately 1% to 15% of the clays were determined. The FS and MS pronounced, they occupied more than 80% of the total sand. By contrast, summation of the VFS+CS+VCS was less than 20%. The MS dominated in Pedon 2, 5 and 6 whilst Pedon 1, 4 and 7 -the FS pronounced. The FS and MS had more or less similar contents for Pedon 3. The MS-dominated soils had less the fine sand to coarse sand ratio [Fi-S/Co-S = (VFS+FS)/(MS+CS+VCS)] than the FSpronounced soils (0.169-2.245 and 1.105-1.947, respectively), and this ratio observed in Pedon 3 was in the middle (0.287-0.99). The rations of the other sizes of the sands to the FS or MS (VFS/FS, MS/FS, VFS/MS, FS/MS and CS/MS) of all pedons revealed similar ranges for the VFS/FS, CS/FS and CS/MS. The MS-pronounced soil had higher MS/FS and lower VFS/MS rations than those on the FS-dominated soils. On the other hand, the FS/MS rations of the FS-dominated soils had trend to be higher than those on the MS-pronounced soils. It could be concluded that the MS and FS contents and their relative rations had high possibility to be the indicators of the aeolian deposited soils. Keywords: Aeolian sand splay landform; Fine sand/Coarse sand ratio; Lower Mun-Chi Basin; Northeast Thailand; Rations of VFS/FS, MS/FS, VFS/MS, FS/MS and CS/MS

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P-27

Parent Material Affecting Phosphorus Distributions in Acid Sulfate Soils of Thailand Pornthiwa KANYAWONGHA1, Nutcharee BOONPLANG1 and Anongnat SRIPRACHOTE2 1

ABSTRACT To study the influence of parent materials on phosphorus distributions in acid sulfate soils of Thailand, eight pedons were collected from different physiographic positions and parent materials. They were the active tidal flat with marine deposits (Pedon 1), the former tidal flat with recent marine and brackish water deposits (Pedon 2) and the former tidal with old marine and brackish water deposits (Pedon 3 to 8). The selected pedons consisted of the potential acid sulfate soils (Pedon 1 and 2), the actual acid sulfate soils with and without jarosite (Pedon 3, 4, 5 and Pedon 6, 7, respectively) and the para acid sulfate soil (Pedon 8). They had clayey texture which the clay and the silt particles occupied more than 90% of the fine earths, in most. Because of low-developing soils, the parent materials had strongly influenced on soil properties, P contents and P forms. The distribution of the organic P more or less followed the organic carbon while the available P similar to the total P throughout the depth. The potential acid sulfate soils contained the highest total P (326 mg kg-1 to 1675 mg kg-1). The actual acid sulfate soils with jarosite had lower the total P than those on the ones without jarosite (71 mg kg-1 to 412 mg kg-1 and 119 mg kg-1 to 663 mg kg-1, respectively). The total P content of the para acid sulfate soil ranged from 169 mg kg-1 to 883 mg kg-1. For fractionation P (SL-P, Fe-P, Al-P, Ca-P), mainly the Fe-P -the highest and the SL-P -the lowest. The variations of fractional P which observed in Pedon 2, 3 and 8 might implied their origin differed. The residual P (= total P residual P) values were higher than the fractionation P indicating that P in acid sulfate soils were mainly unavailable forms. The former brackish water deposits had the lowest total P whilst the recent marine deposits -the highest one. Keywords: Acid sulfate soils, Marine deposits, Brackish water deposits, Phosphorus forms, Thailand

July 1-3, 2015

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Poster Abstracts

Screening of Ametryn Resistant Bacteria from Sugarcane Cultivation Soils Pattrarat TEAMKAO*, Nipaporn ROENGANAN and Siratee PONGPOUN Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: ktpattra@kmitl.ac.th

ABSTRACT Ametryn is a selective systemic herbicide and widely used for the pre-and post-emergence control of weeds in plantations of sugarcane, maize, and banana. In this work, the amount of ametryn resistant bacteria and their diversities in two types of sugarcane cultivation soil with long history of ametryn application were studied. The result showed different bacterial number in two soil types, red-and black-soil. The occurrence of bacteria in red-soil was 2.3x105 CFU/g soil with significantly lower than that of the black-soil (9.2x105 CFU/g soil). The upper red-soil (0-15 cm) had higher amount of bacteria than the lower red-soil (15-30 cm). The diversity of bacteria in black-soil was higher than that obtained from the red-soil, and bacteria found in both soils mostly were gram-negative bacteria. The use of minimal medium (basal salt agar) was appropriated for ametryn resistant bacterial screening than enriched medium (nutrient agar). Keywords: Ametryn, Bacteria, Soil, Sugarcane

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P-29

Effect of Organic Fertilizers on Soil Phosphorus Sorption Capacity Sukunya YAMPRACHA1* and Parichart WATHAKIATTIKUL1 1

ABSTRACT Phosphorus (P) sorption capacity is an important soil property using to recommend P fertilizer for crops. Soil properties have an effect on P sorption capacity including soil organic matter. Therefore, continuous use of organic fertilizers may affect soil P sorption capacity. A pot experiment was conducted for two sweet corn crops on Kamphangsan soil series to study the effect of sources and rates of organic fertilizer on phosphorus sorption capacity. The experimental design was the 3x3 factorials in a randomized complete block design (RCBD) with three replications. Pellet compost, chicken and cattle manure were applied at three different rates i.e. 0, 156 and 312 kg P ha-1. First and second crops were planted during April-June 2013 and August-October 2013, respectively. The soil samples before planting and after second crop harvesting were measured P sorption isotherm by laboratory batch experiment. Langmuir equation was used to describe P sorption capacity and estimated maximum P sorption (Smax) of each soil. The results showed that an application of chicken and cattle manure was significantly increased maximum sorbed P, but the application of pellet compost was decreased. The highest P sorption maximum was found in the soil applied with cattle manure. Soil pH, organic matter, cation exchange capacity, iron, zinc, and manganese were positive correlation with Smax (pvalue<0.01). Although, exchangeable calcium was a negative correlation with Smax (r =-0.68**). However, application rates of three organic fertilizers had no significant effect on maximum P sorption. Keywords: Phosphorus, Sorption, Organic fertilizer

July 1-3, 2015

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P-30

Poster Abstracts

A Relationship between Loss on Ignition and Carbon Concentration Determined by Wet Digestion to Assess C/N Ratio of Plant Materials and Organic Fertilizers Nukoon TAWINTEUNG1* 1

ABSTRACT Loss on ignition (LOI) is a simple method for determining ash and organic matter content of compost and manure. However, only few reported on the correlation between LOI and carbon concentration of plant materials and organic fertilizers in order to assess C/N ratio. One hundred fifty-four organic materials were analyzed for organic carbon by wet digestion (modified Walkley-Black and Graham methods) and LOI. Organic materials consisted of plant materials (119 samples of rice straw) and organic fertilizers (35 samples, including: 18 samples of green-manuring, 8 samples of compost and 9 samples of animal dung) Total nitrogen was analyzed by Kjeldahl method. The result showed that carbon content and LOI were 36.442.3% and 82.3-92.5% for plant materials and 14.4-53.2% and 32.1-96.2% for organic fertilizers, respectively. A linear relationship between LOI and carbon content by wet digestion was highly significant in both plant materials and organic fertilizers (carbon of plant materials (%) = 0.425 x LOI (%) + 2.223; R2 = 0.633, p-value < 0.01 and carbon of organic fertilizers (%) = 0.549 x LOI (%) â&#x20AC;&#x201C; 5.704; R2 = 0.900, p-value < 0.01). The C/N ratio calculated by carbon content from the linear regression and wet digestion was not significant difference by parried sample t-test (p < 0.05). Therefore, LOI can be used to estimate of carbon concentration of both plant materials and organic fertilizers for C/N ratio assessment. Keywords: Loss on ignition (LOI), Carbon, Plant material, Organic fertilizers, C/N ratio

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P-31

Relationship between Total Calcium and Pectin in Asparagus (Asparagus officinalis L.) Cell Wall Nutcharee BOONPLANG1* 1

ABSTRACT Calcium (Ca) is a pre-harvest nutrient that affected on plant quality. Usually, high calcium status in asparagus results in better quality and fewer problems with post-havest rots. The purpose of this research was to study the relationship between total calcium and pectin on cell wall in asparagus. Asparagus spears and leaves were harvested from a commercial farm and trimmed to 180 mm in length and divided into two sections for total calcium and cell wall preparation for total pectin. Total Ca concentrations were determined using ICP-OES, after ashing the samples at 550Â° C for 5 hr and dissolving the ashes in 10 ml 1 N HCl for 1 hr and 10 ml distilled water overnight. Total pectin in asparagus cell wall was hydrolysed using the method of Ahmed and Labavitch (1977) and uronic acids were detected using the assay method of Blumenkrantz and Asboe-Hansen (1973). The relationship of total calcium and pectin on cell wall was slightly high but not significantly different. Keywords: Asparagus (Asparagus officinalis, L.), Calcium, Pectin

July 1-3, 2015

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P-32

Antibacterial Activity of Roselle (Hibicus sabdariffa Linn.) Flower Extract Napaporn KONGKARN1, Pussadee TANGWATCHARIN1, Montinee TEERARAK2, Kanokporn CHANGSAWAKE2 and Komkhae PILASOMBUT1* 1

Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: kpkomkha@kmitl.ac.th

ABSTRACT The objectives of this study were to investigate total content of phenolic compounds and antibacterial activity of water and ethanol roselle (Hibicus sabdariffa Linn.) flower extracts. It was found that total phenolic content was 1262.80 and 675.80 mgGAE/100g DW for water and ethanol extracts, respectively. Various concentrations (6.25, 12.5, 25 and 50 mg/ml) of both extracts were studied for antibacterial activity against 16 bacterial strains by the agar well diffusion method. At the concentration of 50 mg/ml of ethanol extract showed antibacterial activity against all tested isolates, while antibacterial activity of water extract was not observed. However, the effectiveness of extract depended on bacterial strains (inhibition zone 1123.33 mm). Keywords: Antimicrobial activity, Roselle, Agar well diffusion

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Poster Abstracts

P-33

Isolation of ACC-Deaminase-Producing Endophytic Bacteria from Rice (Oryza sativa) Chokchai KITTIWONGWATTANA1* Department of Biology, Faculty of Science, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok, Thailand *Corresponding email: kkchokch@kmitl.ac.th

ABSTRACT Endophytic bacteria are valuable natural resources because of their plantgrowth-promoting activities. One mechanism by which endophytic bacteria promote plant growth is the production of 1-aminocyclopropane-1carboxylic acid (ACC) deaminase which lowers the amount of the phytohormone ethylene. In the present study, endophytic bacteria were isolated from stems and roots of rice collected from Suphanburi, Bangkok and Chonburi provinces, Thailand. One hundred and sixty bacterial isolates were obtained and screened for their ACC-deaminase activity. ACC deaminase production was determined based on bacterial growth on basal medium supplemented with ACC as the sole nitrogen source. The results showed that there were 20 isolates that produced the enzyme. Based on the 16S rRNA gene sequences, ACC-deaminase producers were members of genera Bacillus (6 isolates), Burkholderia (5 isolates), Fictibacillus (2 isolates), Dickeya (1 isolate), Enhydrobacter (1 isolate), Micrococcus (1 isolate), Moraxella (1 isolate), Halobacillus (1 isolate), Staphylococcus (1 isolate) and Ancylobacter (1 isolate). These bacteria are candidates for further study and application as biofertilizers. Keywords: Rice, Endophytes, ACC-deaminase

July 1-3, 2015

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P-34

In vitro Antagonistism of Talaromyces flavus and Neosartorya pseudoficheri Against Anthracnose Disease on Coffee Mayamor SOYTONG1 and Supattra POEAIM1* Department of Biology, Faculty of Science, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: poeaim@hotmail.com

ABSTRACT Thirty isolates of saprobic fungi were obtained from soil in Chiang Mai, Thailand. They were identified into 2 genera based on morphological and molecular analysis, namely, Talaromyces sp. and Neosartorya sp. Three isolates of T. flavus and three isolates of N. pseudoficheri were selected for antagonistic activity testing with Collectotrichum coffeanum by dual culture incubate for 30 days. T. flavus isolate EU05, EU07 and EU16 showed inhibition of C. coffeanum mycelial growth at 68.5%, 63.3% and 60.37%, respectively. N. pseudoficheri isolate EU13, EU35 and EU36 inhibited the C. coffeanum mycelial growth more than 90%. Thus, T. flavus and N. pseudoficheri were shown to be in vitro effective antagonist against C. coffeanum causal of anthracnose disease on coffee. Keywords: Biological control, Collectotrichum coffeanum, Neosartorya sp. and Talaromyces sp.

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P-35

Identification of Blast Resistance Gene in Yang Mawng Variety of Thai Indigenous Rice Siriporn PRAMRIT1,2*and Nonglak PARINTHAWONG3 Department of Agricultural Biotechnology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand 3 Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: siripornpramrit@gmail.com 1

ABSTRACT Rice blast, caused by Pyricularia oryzae, is a major disease of rice almost worldwide. KDML105 is a good quality cultivar, however, this cultivar is susceptible to rice blast disease. Therefore, finding of new rice blast resistance gene is a major approach for rice breeding program. A Thai indigenous rice variety,Yang mawng, is highly resistant to the infection of P. oryzae. In this study, an F2 population was developed from a cross between KDML105 and Yang mawng. Two hundred and twenty eight F2 plants were inoculated with conidia suspension of P. oryzae (105 conidia/mL) and the disease was scored 7 days later. The segregation of resistance and susceptible phenotype showed a goodness of fit to the ratio 15:1. Two hundred and thirty simple sequence repeat (SSR) markers were screened for polymorphism. The result showed that 111 markers showed polymorphism between the parents KDML105 and Yang mawng. Bulk segregant analysis (BSA) was conducted using 111 SSR markers and four markers included RM495, RM431, RM443 and RM543 were obtained. This suggested that, the gene that control blast disease resistance in Yang mawng variety might locate on chromosome 1 of rice genome. The four markers will be used in further analysis of an individual F2 population in order identify the SSR markers linked to blast resistance gene. Keywords: Rice blast, Blast resistance gene, Pyricularia oryzae

July 1-3, 2015

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P-36

Acaricidal Activity of Paracress (Acmella oleracea (L.) R.K. Jansen) Extract on the Mushroom Mite (Dolichocybe indica Mahunka) Jarongsak PUMNUAN1*, Jakkrapong AREEWONG2, Pornprapa KONGTRAGOUL2 and Ammorn INSUNG1 1

Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Program in Horticulture, Department of Agricultural Technology, King Mongut’s Institute of Technology Ladkrabang, Prince of Chumphon Campus, Chumphon 86160, Thailand *Corresponding email: kpjarong@kmitl.ac.th

ABSTRACT Acaricidal activity of ethanol, acetone and hexane extracts from various parts of paracress (Acmella oleracea (L.) R.K. Jansen) including leaf, stem and root against adult of mushroom mite (Dolichocybe indica Mahunka) were investigated by residue contact method. The bioassay was conducted with various concentrations (10.6, 21.2, 31.8, 42.4, 53.0 and 63.6 µg/cm2) in glass tubes, sized 0.4 cm in diameter and 3 cm in length. Seal the ends of the tubes on both sides with filter paper. Ethanol, acetone and hexane were used as the control. Percentages of mortality were examined at 12 hr after application. The results showed that the acetone and hexane extracts from the three parts of paracress were much more effective in controlling mushroom mite. The examination at 53.0 µg/cm2 showed more than 80% mortality with LC50 value of 14.90-35.38 µg/cm2. The acetone extract of paracress root was highly toxic to the mushroom mite with the LC50 of 14.90 µg/ cm2, while mortality of the mite treated by acetone extracts from paracress leaf and stem showed the LC50 of 20.12 and 22.73 µg/ cm2, respectively. Keywords: Residue contact method, Crude extract, Local plant, Spilanthes acmella

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Poster Abstracts

P-37

A Degree-Day Simulation Model for the Population Dynamics of the Brown Planthopper, Nilaparvata lugens Stal. (Homoptera: Delphacidae) Wirote KHLIBSUWAN1, Yupa HANBOONSONG1 and Krirk PANNANGPETCH 1 1

Department of Plant Science and Agriculture Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand *Corresponding email: wirote@kku.ac.th

ABSTRACT The population dynamics of the brown planthopper (BPH), Nilaparvata lugens Stal was simulated based on insect life cycle and weather data. The computer model followed the state variable approach by using thermal requirements of each insect stage as the driving variables. Subsequently, stages and numbers of insect were predicted. Simulated nymphs and adults of BPH were compared with survey data from the field. The model has reasonable predictive value. In time forecasting of the BPH population may help farmers in their decision making process. Simulation using future weather patterns and the influence of global warming on population dynamics of BPH can be predicted. Keywords: Brown planthopper (BPH), Simulation model, Thermal requirements.

July 1-3, 2015

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P-38

Poster Abstracts

Preliminary Study on Used Microalga (Nostoc commune) as a Protein Supplement in Dry-Wet Mixtures Feed for Juveniles Snakehead (Channa striata) Suneerat RUANGSOMBOON* Program in Fisheries Science, Department of Animal Production Technology and Fisheries, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520 Thailand *Corresponding email: krsuneer@kmitl.ac.th

ABSTRACT Snakehead (Channa striata) is a valuable food fish cultured in Thailand. Farms generally produced their own dry-wet mixtures feed on-site each day for snakehead. Farm-made feed is combinations of dry pelleted feed (i.e. commercial feed) and a wet meal containing a variety of feed ingredients. The dry-wet mixtures feed containing microalga, N. commune as a protein supplement for juvenile snakehead was studied. Commercial fish feed was used as a reference diet. Diets containing 0 (control), 10, 20, and 30% of N. commune were used to feed C. striata for 14 weeks. Experimental fish was hand-fed at 5% total body weight twice a day. The optimum inclusion level of N. commune was 10 % with significant highest growth rate, including feed conversion ratio (2.79±0.02), feed conversion efficiency (35.89±0.25), protein efficiency ratio (0.82±0.01), and specific growth rate (2.46±0.04 %/day). An increased N. commune levels negatively affected the growth response of fish while it did not influence the survival rate of fish. The carotenoid content significantly increased with increasing N. commune level. Flesh of C. striata fed with 30% of N. commune contained highest total carotenoid (2.51±0.33 mg/g FW) and protein (73.89±0.06 % DW). C. striata fed with control diet produced significant higher lipid (9.24±0.18 %DW). Additional studies with isoproteic and energetic diets are further recommended to confirm the effect of this culture-microalga on snakehead. Keywords: Channa striata, Fish feed, Nostoc commune, Snakehead

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Poster Abstracts

P-39

Assessing Impact of Artificial Reef on Local Fisherman Household Income Using Propensity Score Matching in Lang Suan District, Chumphon Province, Thailand Rapeepan KANTAVICHAI1*, Monthon GANMANEE1, Thamrong MEKHORA1, Maytapon PORNRATANACHOTSAKUL1 and Ariya THONGSAMUI1 Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: kkrapeep@kmitl.ac.th

ABSTRACT This study attempted to estimate differential impacts on the fishing income in local fisherman households using 92 household survey data after 3 years of artificial reef deployment on coastal of gulf of Thailand in Lang Suan district, Chumphon province. However, the households that have fishing income changes are not random; the estimation of changes in income may be biased by the existence of many other characteristics as confounding factors. Propensity score matching is a way to correct the estimation of effects using for the existence of these confounding factors based on the idea that the bias is reduced when the comparison of outcomes is performed using changed (treated) and unchanged (control) householders who are as similar as possible. We calculated the mean of household income increases and decreases by matching the propensity score derived from household pretreatment characteristics; sub-district, numbers of family member, debt, major marine animal, and boat horse power. The result was useful in socioeconomics aspects of artificial reef investment evaluation. This study was conducted in matching package from R- statistical software. Keywords: Artificial reefs, Propensity score matching, Small-scale fishery, Socioeconomics, Social return on investment

July 1-3, 2015

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Poster Abstracts

P-40

Protein and Some Other Constituents of Sucker Catfish’s Fish Meal Kalkullanutch PATRARASRIPONG1 and Kanok LERTPANICH2 1

Graduate School of Environmental Development Administration, NIDA. Thailand 2 Department of Agricultural Development and Resource Management, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: klkanok@kmitl.ac.th

ABSTRACT The Sucker catfish (Hypostomus plecostomus) is an invasive species in Thailand. Moreover, the Pavesburirom canal, Ladkrabang district, Bangkok especially found the massive numbers of this catfish (Lertpanich and Aranyavali, 2008). The rising population of sucker catfish has been effected to the local fish species. The proper management urgently need for this invasive catfish. However, the production of fish meal from this catfish is regarded. Then, the Sucker catfish’s fish meals are investigated by proximate analysis (AOAC, 1995). The amount of protein, moisture, fat, ash, fiber, calcium and phosphorus were quantified and 3 replications are considered. The results showed the percentage of protein and some other constituents 58.22 % of crude protein, 3.62 % of moisture, 12.34 % of crude fat, 23.20 % of ash, 0.44 % of crude fiber, 8.03 % of calcium and 3.99 % of phosphorus. The Sucker catfish’s fish meal indicated that the protein and some other constituents quality are quite similar to the fish meal standard. However, the higher amount of fat may reduce the quality and delectable. Keywords: Fish meal, Invasive species management, Proximate analysis, Sucker catfish

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Poster Abstracts

P-41

Protein Isolation from Bigeye Snapper (Priacanthus tayenus) Head By-Product Using pH-Shift Method Worawan PANPIPAT* and Manat CHAIJAN Functional Food Research Unit, Department of Agro-Industry, School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand *Corresponding email: pworawan@wu.ac.th

ABSTRACT Bigeye snapper (Priacanthus tayenus) by-products from surimi industry are currently discarded or used for low value fish meal production. Recovery of protein from these by-products is very important to sustainably utilize fishery resources. A pH-shift process has shown to be an efficient method for fish protein isolation. The objective of this study was to evaluate the effect of pHshift process on the physical and chemical characteristics of protein isolate from bigeye snapper head (BSH) by-product. BSH was ground and mixed with cold distilled water at a ratio of 1:9 (w/v). The mixture was adjusted to pH 2.5 or 11.5 using 2 M HCl or 2 M NaOH followed by isoelectric precipitation at pH 5.5 of soluble proteins. From the results, higher protein recovery was observed in the alkaline extraction process compared to the acid counterpart (p<0.05). Alkaline process recovered more pigments resulting in the lowered whiteness of the isolated protein (p<0.05). Both acid and alkaline pH-shift methods effectively removed total volatile basenitrogen and fishy odor from BSH raw material (p<0.05). The oxidation of residual lipids in both protein isolates measured by thiobarbituric acid reactive substances was markedly lower than in BSH raw material (p<0.05). This study revealed that by-product from fishery industry could be fully utilized using pH-shift processing to obtain functional protein. Keywords: Bigeye snapper, By-product, Isolation, pH-shift, Protein

July 1-3, 2015

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Poster Abstracts

P-42

Myoglobin Redox Instability of Protein Isolate from Bigeye Snapper (Priacanthus tayenus) Head By-product Worawan PANPIPAT* and Manat CHAIJAN Functional Food Research Unit, Department of Agro-Industry, School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand *Corresponding email: pworawan@wu.ac.th

ABSTRACT Residual myoglobin plays an important role in whiteness and oxidative stability of fish protein isolate prepared by pH-shift processing. This study aimed to investigate the effect of pH-shift protein isolation on myoglobin redox instability in resulting protein isolates from bigeye snapper head (BSH) by-product. Protein from BSH was isolated using pH-shift processing at pH 2.5 (acid version) or 11.5 (alkaline version) with 2 M HCl or 2 M NaOH followed by isoelectric precipitation at pH 5.5 of soluble proteins. The extractable myoglobin content, metmyoglobin formation and myoglobin spectra in Soret region (A350-A450) in both protein isolates and BSH raw material were monitored. The results showed that the extractable myoglobin content of protein isolates made by both acid and alkaline pH-shift methods was lower than that of BSH raw material (p<0.05) suggesting the efficacy in myoglobin removal of the pH-shift processing. The metmyoglobin content of acid-protein isolate was higher than alkaline one (p<0.05) indicating greater degree of myoglobin oxidation with acid processing. The disappearance of Soret peak was also noticeable in acid version indicating the destruction of myoglobin structure by acid treatment. Therefore, the pH-shift processing, particularly acid version strongly induced the oxidation and degradation of myoglobin. The oxidation of myoglobin and the release of free heme during pH-shift processing can be the factors influencing the discoloration and oxidative instability of protein isolate made from BSH by-product during storage. Keywords: Bigeye snapper, By-product, Myoglobin, pH-shift, Protein

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July 1-3, 2015

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Poster Abstracts

P-43

Utilization of Fish Residue from Fish Sauce Fermentation for High Calcium Sweet Dipping Sauce (Nam Pla Wan) Production Manat CHAIJAN*, Worawan PANPIPAT and Waraporn TUMTONG Functional Food Research Unit, Department of Agro-Industry, School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand *Corresponding email: cmanat@wu.ac.th

ABSTRACT The traditional process for the manufacturing of fish sauce begins with mixing salt together with fish. The fish-salt mixture is stored at ambient tropical temperatures for 6-12 months in large concrete tanks. A water soluble protein-rich fraction called fish sauce is formed with increasing fermentation time due to the action of digestive and bacterial enzymes. The residue from fish sauce fermentation is usually composed of fish frame which contains high contents of calcium and phosphorus. This study aimed to utilize fish residue from fish sauce fermentation for production of high calcium sweet dipping sauce (Nam Pla Wan). Dried fish residue powder can be added into the sweet dipping sauce at a concentration of 5% (w/w) in the presence of sucrose. This formulae rendered the sauce with highest preference score (p<0.05). Calcium and phosphorus contents of sweet dipping sauce added with 5% dried fish residue powder were higher than those of commercial one (Takrai brand) (p<0.05). In addition, the aw, total plate count and total yeast and mold of sweet dipping sauce added with 5% dried fish residue powder met the requirement of the Standard of Thai Local Products. The shelf-life study revealed that this product can be stored for 47 and 69 days at 30 and 25ď&#x201A;°C, respectively. Keywords: Calcium, Dried fish residue, Fish sauce, Sweet dipping sauce

July 1-3, 2015

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P-44

Poster Abstracts

Genetic Diversity of Seagrasses across the Eastcoast of Thailand Based on Sequence-Related Amplified Polymorphism (SRAP) Technique Pattama SRINAMNGOEN1* and Kanok-on DUANGPAKDEE1 1

Faculty of Science and Art, Burapha University, Chanthaburi Campus, Chanthaburi 22170, Thailand *Corresponding email: pattama@buu.ac.th

ABSTRACT Seagrasses have a long evolution and adaptation to become a marine flowering plant, and also have high impact on marine ecosystem. The identification of seagrasses was classified using the functional group, not a taxonomic group. Because, the morphology and phenotypic can be changed by the environment. Genetic diversity can be measured by genetic variability. So, SRAP technique was investigated to detect the variability of seagrass genome. Twenty-six samples of six seagrass species from the eastcoast of Thailand including Chonburi, Rayong, Chanthaburi and Trad provinces were collected. The result showed that 12 out of 25 primers pairs produced clear polymorphic patterns and showed highly polymorphism due to locations and environment. According to the genetic relationship and cluster analysis, the phylogenetic tree was constructed and the similarity coefficient was 0.93. The phylogenetic tree was divided into 2 major groups (or 4 minor groups). The first major group, elliptic short leaf blade, was Halophila ovalis from Trad province. The second major group, having acuminate long leaf blade, included 5 species, namely Cymodocea serrulata, Halodule uninervis, H. pinifolia and Enhalus acoroides. Although the large variability of seagrasses within species and also between species was detected, the genetic diversity showed a correspondence with the morphological identification as well. Keywords: Seagrass, SRAP, Phylogenetic tree, Genetic variability

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July 1-3, 2015

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Poster Abstracts

P-45

Genetic Variation of Halodule pinifolia Collected from Rayong Province Using SRAP Marker Siraphop ADICHANUND1 and Pattama SRINAMNGOEN1* 1

Faculty of Science and Art, Burapha University, Chanthaburi Campus, Chanthaburi 22170, Thailand *Corresponding email: pattama@buu.ac.th

ABSTRACT The seagrass Halodule pinifolia is wildly distributed along the coastline and play an important role in an ecological habitat. The H. pinifolia is listed as a â&#x20AC;&#x153;Critically Endangeredâ&#x20AC;? species. The objective of this research was to estimate the genetic variation of 7 samples of the H. pinifolia that were collected from Rock garden community and Chom Ta-wan coast, Rayong province. Sequence-related amplified polymorphism (SRAP) technique was applied as a samples method. It was found that 5 out of 12 pairs of primers produced clear 17 polymorphic bands from a total of 41 bands (average of 8.2 bands/primer). According to this result, the genetic variation of this sampling is 40%. From BLASTX analysis, HaP5 SRAP fragment that generated by ME5/EM2 SRAP primer was highly similar to Trypsin-like protease Superfamily (Tryp_SPc) conserved domain. It may be expressed in male gametes of both plants and animals and it is largely divergent in the both kingdoms. So, the result indicated that the environment has high impact on genetic variation of H. pinifolia for its evolution and adaptation. Keywords: Genetic variation, Halodule pinifolia, SRAP

July 1-3, 2015

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2 International Symposium on Agricultural Technology (ISAT2015)

P-46

Poster Abstracts

Aquatic Polyculture Farm in Bangsaothong District, Samutprakarn Province, Thailand Nipon JITTAMNAN1* and Panneepa SIVAPIRUNTHEP2 1

Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520 Thailand 2 Department of Agricultural Education, Faculty of Industrial Education, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520 Thailand *Corresponding email: kjnipon@gmail.com

ABSTRACT Direct observation, interview, and discussion with a selected aquatic polyculture farmer was conducted to collect data regarding which kind of fish and shrimp to be cultured, how to culture and manage aquatic polyculture farm, how much of yield, the cost and profit of the investment, and etc. Data were analyzed using descriptive statistics and it was then described to be the knowledge for polyculture farming as a sustainable aquaculture. Aquatic polyculture farm is the most popular aquaculture farming in Bangsaothong district, Samutprakarn province, Thailand because of the occurrence of natural food sources such as phytoplankton, zooplankton, aquatic insect, and benthos for feeding the fish and shrimp. Thus, the aquatic polyculture farming is the method to use all natured food. In addition, the investment cost of aquatic polyculture farming is low and the return is high. The results of this study revealed that 3 types of herbivorous fish namely, Tilapia (Oreochromis niloticas), Rohu (Labeo rohita), Bighead carp (Aristichthys nobilis (Richardson)) and the Vannamei shrimp (Litopenaeus vannamei) were cultured in this aquatic polyculture farm. Vannamei shrimp was cultured to marketable size and sold every 2 months while fishes were harvested after 8-10 months of the culturing. The advantages of this aquatic polyculture were the maximum utilization of natural food in the pond by fish and shrimp and no accumulation of waste and disease thanks to the utilization of all organic residues by Vannamei shrimp. Keywords: Herbivorous fish, Vannamei shrimp, Aquatic polyculture, Natural food

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Poster Abstracts

P-47

Microbiological Safety of Ready-to-Eat Semi-Dried Nham, an Innovation of Thai Fermented Meat Product Thanapa CHETAWAN1, Komkhae PILASOMBUT1* and Supaluk SORAPUKDEE1 1

Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: komkhae@yahoo.com

ABSTRACT Semi-dry Nham is an innovation of Thai fermented meat product. The objective of this study was to determine microbiological contamination in semi-dry Nham. Semi-dry Nham was prepared by a fermentation process at 30 ºC for 3 days and subsequently dried in an oven to reach core temperature at 71 ºC. The experiment was composed of control and 2 treatments: adding 5% and 10% glycerol to Nham. The numbers of Staphylococcus aureus, yeast/mold, and lactic acid bacteria were determined as well as pH and total acidity. The results showed that after 3 days of fermentation, the number of S. aureus and yeast/mold were not observed. The numbers of lactic acid bacteria in control and treatments increased progressively after 3 days of fermentation. On the other hand, lactic acid bacteria S. aureus and yeast/mold were not found in any groups after the Nham was dried. The pH value of the 10% glycerol treatment was higher than those of the other two groups, and the total acidity of the 10% glycerol treatment was lower than those of the other groups. Hence, this study indicated that ready-to-eat semidry Nham prepared as above-mentioned would be safe from pathogenic bacteria. Keyword: Semi-dry Nham, Fermented meat product

July 1-3, 2015

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2 International Symposium on Agricultural Technology (ISAT2015)

P-48

Poster Abstracts

Carcass Traits Comparison between Duroc and Commercial Crossbred Pigs Numfon TAJASRI1*, Chanporn CHAOSAP2, Ronachai SITTHIGRIPONG1 and Rutcharin LIMSUPAVANICH1 1

Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand 2 Department of Agricultural Education, Faculty of Industrial Education, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: numfont.j@gmail.com

ABSTRACT Carcass traits of Duroc (DR), DR x [Large White x Landrace] 1 (DLL1) and DR x [Large White x Landrace] 2 (DLL2) crossbreds were studied. Thirty pigs were randomly distributed into 3 treatments, with 5 barrows and 5 gilts in each. All pigs were reared under the same housing and feeding condition for 6 months prior to slaughter at 110±5 kg. live weight. After slaughter, carcass traits were evaluated. Loin eye area (LEA) was measured and marbling score was determined on Longissimus muscle between the 10th and 11th ribs from the left side of each carcass. Results showed that there were no significant differences (P>0.05) in live weight, hot carcass weight, carcass length, LEA, backfat thickness, FOM grading and lean percentage among breeds. DR had the highest (P<0.05) marbling score and leg percentage, whereas DLL2 had the lowest (P<0.05) trimmed fat. Gilts had higher (P<0.05) dressing percentage, belly and trimmed fat than barrows. However, barrows had higher (P<0.05) marbling score than gilts. Comparing to DLL1 and DLL2, DR showed excellent marbling but no significant difference in lean percentage. Keywords: Carcass traits, Duroc, Crossbred pigs, Lean percentage, Marbling

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Poster Abstracts

P-49

Optimization of Culture Conditions of Bacteria Isolated from Buffalo Rumen for Cellulase and Xylanase Production Kanya JIRAJAROENRAT1*, Jutatip CHALERMWANICHWONG2, Tipaporn NGAMSANGA2 and Kanokrat SRIKIJKASEMWAT1 1

Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand 2 Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: kjkanya@kmitl.ac.th

ABSTRACT Cellulase and xylanase producing bacteria were isolated from swamp buffalo rumen. The bacterial isolate ID2R1P1 showing a clear zone on both CMC agar and xylose agar was selected. Optimum concentration of substrates, pH, temperature and incubation time for cultivation of the isolate to maximize the production of cellulase and xylanase enzymes were determined. The results indicated that optimal cellulase production was carried out by incubation of the isolate in a broth containing 1% CMC using yeast extract as a nitrogen source, at pH 7.0 and 39°C for 2 days. Maximum cellulase yield was 2.034±0.023 U/ml. Optimal condition for producing the xylanase enzyme was incubation of the isolate in a broth adding yeast extract and 2% xylan at pH 3.0 and 37°C for 2 days. Maximum xylanase yield was 183.272 ± 0.015 U/ml. Keywords: Cellulase, Xylanase, Buffalo, Ruminal bacteria

July 1-3, 2015

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P-50

Poster Abstracts

Consumption of Energy, Water and Consumables in Pon-Yang-Kham Beef Cutting Process Chalermsak SAKDAPECHSIRI1, Thierry TRAN2, Matana OSOTHONGS3 and Kanya JIRAJAROENRAT1* 1

Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 QUALISUD, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), 34398 Montpellier Cedex 5, France 3 Pon-Yang-Kham Livestock Breeding Cooperative NSC., Ltd., Pathum Thani 12130, Thailand *Corresponding email: kjkanya@kmitl.ac.th

ABSTRCT Pon-Yang-Kham or Thai-French beef is well known as a premium beef product especially as steak in Thailand modern trade. To generate the environmental friendly production process, we investigated the consumption of energy, water and consumables during the production from farming to packaging of the product. In the cutting process, energy consumption included diesel for truck and electricity. Consumables include clothes, cleaning equipment, cleaning chemicals, cutting equipment and packaging equipment. The data were collected during year 2010-2014. The results showed that half carcass about 2,000 ton/year or 163 ± 10 ton/month entered the cutting process. After cutting, boneless beef was about 480 ton/year. During the cutting process, consumption of electricity was 0.423 kWh/kg of beef. Consumption of water was 9.56 litre/kg of beef. Diesel used by truck for delivery of beef to supermarket was 0.019 litre/kg of beef. In packaging, plastic bags including low density polyethylene (LDPE), high density polyethylene (HDPE) were used about 10 ton/year. Moreover, booth shoe made of polyvinyl chloride (PVC), foam box made of polystyrene were used. The emission factor of all equipment will be further analyzed. Finally, life cycle assessment (LCA) of Pon-Yang-Kham beef production will be analyzed and also the carbon footprint of the product will be reported. Keywords: Beef, Emission factor, Life cycle assessment (LCA), Carbon footprint

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Poster Abstracts

P-51

Gel Properties of Pork Ball Containing Mixed Precooked Carotenoid-rich Vegetables Manat CHAIJAN*, Worawan PANPIPAT, Noppawan LAOPHROM and Saowanee TRUKTRONG Functional Food Research Unit, Department of Agro-Industry, School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand *Corresponding email: cmanat@wu.ac.th

ABSTRACT Carotenoid-rich vegetables like carrot, tomato and corn can be used as a source of natural coloring agent, fiber and antioxidant in meat products. However, the presence of these vegetables could impair the gel-forming ability of myofibrillar proteins in gel-type products. This study aimed to investigate the effect of mixed precooked vegetables (MPV) (carrot: tomato: corn, 1:1:1 w/w/w) on gel properties of pork ball. The gels of original pork ball without addition of MPV (control) and pork ball containing 20% ground MPV were produced by incubating the sol at 60ยบC for 30 min followed by heating at 70ยบC for 20 min. The resulting pork ball gels were analyzed for pH, breaking force, deformation, color (L*, a* and b* values), expressible moisture and acceptability. The results showed that the pH, breaking force, deformation, L* value and some sensorial properties (odor, taste, texture and overall liking) of both pork ball gels were not significantly different (p>0.05). The expressible drip of pork ball gel with MPV was higher than control (p<0.05) indicating a lower water holding capacity of gel with MPV. The pork ball gel with MPV had higher a* and b* values with significantly increased color score (p<0.05). Therefore, mixed precooked carotenoid-rich vegetables can be added to pork ball formulae for producing alternative functional meat product without the detrimental effect on acceptability. Keywords: Color, Gel-forming ability, Pork ball, Vegetable

July 1-3, 2015

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Poster Abstracts

P-52

Changes in Physicochemical, Microbiological and Sensorial Quality of Jerky Processed From Spent Laying Hen Meat during Storage Chanpen UESAKULRUNGRUENG, Supaluk SORAPUKDEE* and Komkhae PILASOMBUT Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: kssupalu@kmitl.ac.th

ABSTRACT The objective of this study was to evaluate the quality deterioration of jerky produced from spent laying hen meat during 90 days. Reformed jerky product was packed in vacuum packaging, stored at room temperature and then collected at day 0, 30, 60 and 90 for determination of shear force, color, water activity, lipid oxidation, microbiological content and sensory evaluation. The result showed that texture of products stored at 60 and 90 days had higher shear force value than those stored at 0 and 30 days (P<0.05). Regarding color of jerky, lightness value increased, while redness and yellowness values decreased during prolong storage (P<0.05). There were no significant differences in lipid oxidation as indicated by TBARS and water activity during 90 days (P>0.05). The number of total plate count, yeast/mold and S. aureus of product along 90 days was less than limit of detection. It was indicated that jerky product was stable to micro-organisms that can cause product spoilage and poisoning. Finally, the hedonic score of jerky stored at 90 days was similar to those stored at day 0 (P>0.05), which refer to the acceptability of product. In conclusion, jerky product was stable to microbiological and chemical changes during 90 days of storage, but some discoloration and tough texture was observed. Keywords: Humectants, Semi-dried meat, Meat product

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July 1-3, 2015

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Poster Abstracts

P-53

Screening of Lactic Acid Bacteria from Traditional Fermented Meat Products Jirarot NITHISANTAWAKHUPT, Pussadee TANGWATCHARIN* and Kan SUKSUPATH Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand *Corresponding email: putang3009@hotmail.com

ABSTRACT The aim of this study was to screen of lactic acid bacteria (LAB) from 22 traditional fermented meat products which were 6 Thai fermented sausages and 16 Nhams, produced from different factories. They were collected from 12 local markets in Bangkok, Thailand. LAB was isolated on MRS agar by pour and streak plates. They were confirmed as LAB by catalase test, gram stain and cell shape. The results showed that 167 isolates of LAB were identified as 135 (80.84%) isolates of gram-positive cocci and 32 (19.16%) isolates of gram-positive rods. This study found that LAB isolate numbers were ranged in 6.00 - 8.50 isolates per sample. Thus, traditional fermented meat products are the major source of LAB. Keywords: Screening, Lactic acid bacteria, Traditional fermented meat products

July 1-3, 2015

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Poster Abstracts

P-54

Screening and Identification of Lactic Acid Bacteria with Antimicrobial Activity to Mastitis Pathogen in Dairy cows; Staphylococcus aureus Khakhanang RATANANIKOM1, *, NantiyaSUWANPANYA1, Yupaporn KHONNALAO1 and Sarayut SRIROD1 1

Faculty of Agro-Industrial Technology, Rajamangala University of Technology Isan, Kalasin Campus, Thailand *Corresponding email: khakhanang_r@yahoo.com

ABSTRACT This study aimed to screen and identify of Lactic acid bacteria which are capable of inhibiting the growth of Staphylococcus aureus, a common dairy cows Mastitis pathogen. A total 250 bacterial strains were isolated from 60 raw milk samples in Kalasin province. The differences in antimicrobial activity against S. aureus among these isolates were found when performing the agar-disc diffusion technique. Five isolates, showing the dominant inhibitory efficiency to the indicator strain were chosen for biochemical test. The results exhibited that all five isolates were gram positive, catalasesnegative microorganisms and identified as Lactobacillus spp. by using 16s rDNA sequencing. The inhibitory efficiency was described as percentage of inhibition ratio. Their ratios of inhibitory activity were in the range of 25.3231.37ď&#x20AC;Ľ, compared with commercial antimicrobial drug. Bacterial growth, pH change and stability in high temperature condition as well as protease treatment were also studied. It clearly showed that productions of antimicrobial activity by these five isolates in MRS medium followed a growth-associated pattern. The pH change from as about 5.6 to as about 4.2 during 24 hours led the inhibitory activity to the highest and then remained gradually stable. High temperature and protease treatment of lactic acid culture indicated that heating and protease had no effect on their antimicrobial activity. On the other hand, pH adjustment in lactic acid culture to 7.0 exhibited no anti-S. aureus activity, implying that the acidic condition, produced by lactic acid bacteria is one of the mechanism for inhibiting the growth of dairy cow-Mastitis pathogen. Keywords: Antimicrobial activity, Cow-Mastitis pathogens, Lactic acid bacteria

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July 1-3, 2015

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Poster Abstracts

P-55

Comparison of Stomoxys calcitrans (Diptera: Muscidae) Attacked and Behavioral Responses on Genetic Blood Differences of Holstein-Friesian Dairy Cattle in Thailand Ubon TANGKAWANIT1*, Varangrat SENASING1, Wirote KHLIBSUWAN1, and Tassanee JAMJANYA1 1

Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand *Corresponding email: ubonta@kku.ac.th

ABSTRACT Fourteen dairy cows in each genetic blood levels of 75-87.5%, >87.5-93.8% and >93.8-100% Holstein-Friesian were examined for the number of stable fly attack and the behavioural responses during November 2009 to October 2010. Stable flies attack was studied by counting flies from the front leg within 2 min. Then, the following four behaviours of tail flick, skin twitch, leg stamp and head throw were observed in each genetic blood level within 3 min. The results showed statistically significant for the number of fly attack. The genetic blood level of >93.8-100% Holstein-Friesian dairy cow was less attacked than the others. For the behavioural responses, 75-87.5% HolsteinFriesian showed the highest frequencies of behavioural responses. The correlation between fly number and fly repelling behaviour was high, except head throw (r2=0.12). The number of stable fly attacked dairy cattle was highest on July 2010 and lowest on March 2010. The information of population dynamic of stable fly and behavioural responses of different blood level of Holstein-Friesian cross breed to stable fly are useful for the farmer to select suitable percentage of genetic blood level of crossbred Holstein-Friesian dairy cattle and to aware the disease and the fly attack in stable fly season. Keywords: Stable flies, Holstein-Friesian, Behaviour, Dairy cow

July 1-3, 2015

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P-56

Poster Abstracts

Opinion Toward Agricultural Further Study of Student in Nongchok District, Bangkok Metropolis Tippawan LIMUNGGURA1* and Panya MANKEB1 1

Department of Agricultural Development and Resource Management, Faculty of Agricultural Technolog, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand *Corresponding email: kltippaw@kmitl.ac.th

ABSTRACT The purposes of this paper were to explore the opinion toward agricultural further study of student and factors relating to the student opinion in Nongchok district, Bangkok Metropolis. Data were collected by questionnaires from 358 sixth grade secondary school students in 3 school; namely, Nongchok Pittayanusorn Matthayom, Bordindaecha (Sing Singhasaenee)4 and Matthayom Wat Nongchok. Data were analyzed by descriptive statistics, t-test and F-test. The findings revealed that the opinion toward agricultural further study of student had medium level (Grand mean 3.28) and the important factors relating to their opinions were school )p≤ .01), religion and family occupation )p≤ .05). Key words: Opinion Toward Agricultural Further Study, Student, Nongchok district, Bangkok Metropolis

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Poster Abstracts

P-57

Using Augmented Reality (AR) Technology for the Promotion of Agricultural Products: A Case Study of Bang Krathum Processed Banana Product Nutthakorn SONGKRAM1* 1

Department of Agricultural Development and Resource Management, Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok, Thailand *Corresponding email: ksonutth@kmitl.ac.th

ABSTRACT The purpose of this research was to study the application of augmented reality (AR) technology to design poster and packaging labels of Bang Krathum processed banana products. The methods used in this research were as follow: 1) To interview the five experts for their opinion about the use of augmented reality technology for the promotion of agricultural products. 2) To develop poster and packaging labels of Bang Krathum processed banana products. 3) To evaluate poster and packaging labels by five experts and improve them based on their recommendations. 4) To survey the poster and packaging labels sampling with a group of 50 people and assess their satisfaction. The data were analyzed by using frequency, percentage, mean and standard deviation. The results of the study revealed that the samples were satisfied at the highest level (mean 4.64, SD 0.49). In addition, they also thought that using AR technology: 1) could make the products trustworthy. 2) enhanced agricultural products standard in Thailand. 3) easy to use. 4) provided information of products clearly. 5) built brand identity 6) stimulated the customers to purchase.

Keywords: Augmented reality (AR), Promotion of agricultural products, Poster and packaging lebel design.

July 1-3, 2015

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P-58

Poster Abstracts

Factors Affecting Consumer Purchasing Behavior of Toxic-Free Vegetables in Muang District, Samutprakarn Province Panya MANKEB1*, Pratompong MATONG1, Suneeporn SUWANMANEEPONG1 and Prapaporn CHULILUNG2 Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Faculty of Science and Technology, Valaya Alongkorn Rajabhat University, Khong Luang, Pathum Thani 13180, Thailand *Corresponding email: kmpanya@kmitl.ac.th

ABSTRACT Interest in toxic-free vegetables has grown substantially as the consumers react to the media about food safety. The purposes of this study were to investigate consumer purchasing behavior and factors affecting consumer purchasing behavior of toxic free vegetables in Muang district, Samutprakarn province. Questionnaires were used to collect data from 405 respondents. Data analysis was conducted by descriptive statistics such as frequency, percentage, arithmetic mean and standard deviation. Hypothesis tests were applied using t-test, F-test and multiple regression analysis. The results showed that the majority of respondents were female, 45-54 years old, college-educated, married, employed in private business, and personal income greater than 40,000 baht per month. The rationale to buy free toxic vegetables was health concern. Most information was perceived by television media. The average purchasing frequency was once a week at 0.86 kg. and 107.22 baht for each purchase. The significant factors (p<.05) affecting purchasing behavior of toxic-free vegetables were age, marital status, monthly income, product, distribution channels and marketing promotion. Keyword: Consumers purchasing behavior, Free toxic vegetables

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Poster Abstracts

P-59

Financial Cost and Benefit Analysis of Commercial Orchid Farming in Krathumban District, Samutsakorn Province, Thailand Panya MANKEB1*, Chalida LERTKRASEMPON1, Tippawan LIMUNGGURA1 and Prapaporn CHULILUNG2 Faculty of Agricultural Technology, King Mongkutâ&#x20AC;&#x2122;s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Faculty of Science and Technology, Valaya Alongkorn Rajabhat University, Khong Luang, Pathum Thani 13180, Thailand *Corresponding email: kmpanya@kmitl.ac.th

ABSTRACT The main objective of this study was to assess the financial return of orchid farming in Kratumban district, Samutsakorn province, Thailand. The results showed that the total cost of orchid investment in 10 rai (1.6 ha.) for 4 years was 1,897,096.84 baht, while total benefits generated by the investment was 2,339,291.67 baht and net profit was 442,194.83 baht. The financial analysis showed that the net present value (NPV) was 3,925,028.51 baht. The benefitcost ratio (BCR) was estimated to be 1.65. The internal rate of return (IRR) on investment was found high at 52.56 percent. These results of financial analysis indicated that the orchid farming investment was financially worth and profitable because 3 basic of financial measures were acceptable; NPV of the investment was positive, BCR was greater than 1 and the IRR was greater than the opportunity cost of investment (7 percent). Keyword: Orchid, Financial cost and benefit analysis

July 1-3, 2015

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P-60

Poster Abstracts

Enhance Environment of a Historic Site, Ancient Benhama Maharat Building, For Future Public Use Warong NAIVINIT 1*, Parkpoom SUEBNUKARN1, Rukkeit SANPRASERT1 and Taweesak WIYACHAI 1 1

Faculty of Agriculture, Ubon Ratchathani University, Thailand *Corresponding email: wnaivinit@yahoo.com

ABSTRACT For a century, the Benchama Maharat School has undertaken to educate the youth of Ubon Ratchathani and other nearby provinces. In 1935, Benchama Maharat School student roll reached its limit. To accommodate the growing number of students, the school was moved to a new site. A new school building was constructed on the west side of Tung Sri Meuang field. It is a two-story Manila architectural style building and completely built of hard wood and teak on a cement base. Its design is influenced by the Dutch and Thai architecture. Because of the uniqueness of the ancient Benchama Maharat building, the entire block was registered by the Fine Arts Department as a national historic site. Not only is the history and identity of the ancient building marvelous, but also the location of this site has high potential for public use as a large green space to improve metropolitan quality of life. However, without strategic plan, this site has been being misused. The objective of this design project was to enhance the environment of this site to be a new provincial landmark. The concept is to integrate the contemporary landscape design principles into colonial garden design. Our design solution would at best be an effective public notification to stimulate discussion among prospective users. This master plan will be a design guideline to help people visualize and imagine the future look and feel of this public space. Subsequently, collective agreements among people of Ubon Ratchathani to develop this site would be generated through the use of this design in participatory process. Keywords:

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Benchama Maharat, Collective agreements, Green space, Historic site, Metropolis environment

July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

P-61

Poster Abstracts

Biodiesel Synthesis from Used Frying Oil with the Modified Catalyst from Waste Anadara granosa Shells Sasiwimol WOOTTHIKANOKKHAN*, Buppha THAMNIYOM and Supamas CHUANOI Department of Chemistry, Rajamangala University of Technology Krungthep, Sathorn, Bangkok, Thailand * Corresponding email: sasiwimol.w@rmutk.ac.th

ABSTRACT Biodiesel is an alternative energy resource obtained from plant. Recently, development of low cost manufacturing process is remarkably crucial in order to solve the production cost. In this research, biodiesel synthesis from used frying palm oil with waste Anadara granosa shells as the modified catalyst was investigated. Preparation of catalyst started from calcinations of waste A. granosa shells at 1,000ď&#x201A;°C for 2 hrs following by impregnation with ammonium carbonate solution and heating again at 600ď&#x201A;°C for 2 hrs. The catalyst was then, tested for its trans-esterification catalytic activity by varying the volume of catalyst ranging from 0.5 to 2% w/w, molar ratio of methanol to oil at 9:1 and 12:1, and reaction time between 1 and 5 hrs. The results showed that the catalyst derived from waste A. granosa shells and impregnated with ammonium carbonate solution, exhibited high activity in biodiesel synthesis, where the optimum condition was found with corresponding to the molar ratio of methanol to oil at 9:1 for 1 hr upon 1% w/w of the catalyst yielding the highest methyl ester of >90% w/w. A. granosa shell is, then, a valuable source for the production of heterogeneous base catalyst that can be successfully utilized for the synthesis of methyl ester biodiesel. Keywords: Biodiesel, Methyl ester, Anadara granosa shells, Heterogeneous catalyst

July 1-3, 2015

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Poster Abstracts

P-62

Scanning Electron Microscope of Alkaline Treated Spent Coffee Ground Chayaporn WONGSIRIDETCHAI1, Watcharaphan CHIANGKHAM1, Narissara KHLAIHIRAN1 and Sudathip CHANTORN1* 1

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Pathum thani, Thailand *Corresponding email: t_sudathip@yahoo.com, sudathip@tu.ac.th

ABSTRACT Coffee beverage consumption has been increasing dramatically. From the coffee beverage manufacturing, a solid residue known as spent coffee grounds (called SCG) is discarded as a solid waste. In recent years, the increasing awareness of the need for waste reduction and environmental protection has stimulated the search for possible methods of using this waste. The compositions of SCG are lignocellulosic materials. The lignocellulose can be digested by lignocellulase. However, the presence of lignin in lignocelluloses leads to a protective barrier that prevents plant cell destruction by fungi and bacteria. Therefore, a pretreatment processing step is required for depolymerization of lignin before conversion of cellulose and hemicellulose into simple sugars. In this research, SCG was treated by 0.5 N sodium hydroxide at 50, 60, 70, 80, 110 and 121ď&#x201A;°C for 6 hrs. Scanning electron microscope (SEM) studies revealed treated-SCG surfaces. Our SEM results showed that the treated-SCG was partially destroyed. In addition, treated-SCG was digested by crude enzyme from Bacillus sp. GA2 (1). The amount of reducing sugar was analyzed by dinitro-salicylic acid method. Amount of reducing sugar from treated-SCG was higher than untreatedSCG. Keywords: Spent coffee ground, Lignocellulose, Pretreatment, Scanning electron microscope

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July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Poster Abstracts

P-63

Production of α-Amylase by Newly Isolated Thermotolerant Bacillus aerius through Solid State Fermentation by Using Some Agricultural Wastes Veysi OKUMUŞ1*, Sadin ÖZDEMİR1 and Abdurrahman DÜNDAR2 1

Biology Department, Science and Art Faculty, Siirt University, Kezer, 56100, Siirt, Turkey 2 Medical Promotion and Marketing Program, Vocational Higher School of Health Service, Mardin Artuklu University, Mardin, Turkey *Corresponding email: veysiok@hotmail.com

ABSTRACT This study was concerned with production of α-amylase by newly isolated thermotolerant Bacillus aerius VO-8 (Accession:KJ842091.1) through solid state fermentation method (SSF) by using some agricultural wastes as substrates. Solid substrates such as rice husk, corn cob, lentil bran and wheat bran were studied for enzyme production. The highest enzyme production was obtained from using wheat bran under SSF environment. The effects of different solid state fermentation conditions such as particle size, incubation time, temperature, pH and surfactants on α-amylase production were experimented. The maximum α-amylase production was observed as 1,632 U/mg at 72 h and 1500 μm particle size. The optimum temperature and pH value of the α-amylase was found 65 °C and 6.0, respectively. Keywords: Agricultural waste, α-Amylase, Bacillus aerius, Solid-state fermentation (SSF), Thermophilic bacteria

July 1-3, 2015

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2 International Symposium on Agricultural Technology (ISAT2015)

List of Participants

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2 International Symposium on Agricultural Technology (ISAT2015)

List of Participants

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40.

Abdul Salam BABJI Allah Wadhayo GANDAHI Alwani HAMAD Ammorn INSUNG Anchanok ONDEE Apisit SANLA Chalermsak SAKDAPECHSIRI Chamroon LAOSINWATTANA Chandra Prakash KHARE Chanpen UESAKULRUNGRUENG Chayaporn WONGSIRIDATCHAI Chitphan KAEWME Chokchai KITTIWONGWATTANA Chulalak TALUBNAK Duangkamol PANROSITP THUNMATIWAT Dusit AUE-UMNEOY Eakkarin SUKKAEW Fontip NUUDOM Fung Lewis Fu Uh Giang NGUYEN THIEN TROUNG Gray WILLIAMS Hariyadi JAMIN PRIYO MINARJO Hideo ISHII Hien Huu NGUYEN Hiroyuki KONUMA Isao TSUTSUI Janine CROSER Jarongsak PUMNUAN Jegan REVATHI Jindarha PREAMPRAMOTE Jirarot NITHISANTAWANKUPT Julian WISEMAN Kampanat PHESATCHA Kanchanapa KAMCHAN Kanda LOKAEWMANEE Kanet JAIKENGKAJ Kanjana SAETIEW Kannikar KAEWSONGSANG Kanok LERTPANICH Kanokporn CHANGSAWAKE

July 1-3, 2015

Malaysia Pakistan Indonesia Thailand Thailand Thailand Thailand Thailand India Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Cameroon Thailand China Indonesia Japan Thailand FAO Thailand Australia Thailand India Thailand Thailand United Kingdom Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand

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List of Participants

41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. 79. 80.

Kanthee SIRIVEJABANDHU Kanya JIRAJAROENRAT Kasilingam PRAKASH Kawewat THONGSURIVONG Khakkhanang RATANANIKOM Khanitta SOMTRAKOON Kitti BOONLERTNIRUN Komkhae PILASOMBUT Krisana RUNGROJWANICH Lampan KHURNPOON Laxmana Swamy SINGAM Malatee PRADUBYART Manat CHAIJAN Mayamor SOYTONG Metha WANAPAT Monthon GANMANEE Montinee TEERARAK Napaporn KONGKARN Nattawan BUSSABA Natthakiti PHURUEN Nipaporn YONSAWAD Nipon JITTAMNAN Nittaya PHAKAMAS Nonglak PARINTHAWONG Noppadol PANCHAN Nukoon TAWINTEUNG Numfon TAJASRI Nutcharee SONGSAKUL Nutcharee BOONPLANG Nutthakorn SONGKRAM OkumuĹ&#x; VEYSI Omid SADEGHIPOUR Panya MANKEB Pariyaporn NETSAWANG Pattama SRINAMNGOEN Pattharin WICHITTRAKARN Pattrarat TEAMKAO Paveena TAWEEKIJAKARN Peerachai KULLACHAI Phatchara EAMKIJAKARN

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Thailand Thailand India Thailand Thailand Thailand Thailand Thailand Thailand Thailand India Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Turkey Iran Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand

July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

List of Participants

81. 82. 83. 84. 85. 86. 87. 88. 89. 90. 91. 92. 93. 94. 95. 96. 97. 98. 99. 100. 101. 102. 103. 104. 105. 106. 107. 108. 109. 110. 111. 112. 113. 114. 115. 116. 117. 118. 119. 120.

Phawinee KAMSAN Pongsakorn NITMEE Pornpairin RUNGCHAROENTHONG Pornprapa KONGTRAGOUL Pornthiwa KANYAWONGHA Prommart KOOHAKAN Rajeev BHAT Rangsan SOISOM Ranyikar PORAHA Ronachai SITTHIGRIPONG Rungrat VAREEKET Rungtawan YOMLA Rutcharin LIMSUPAVANICH Sakchai CHOOCHOTE Sakchai WUTHIMANOP Sarayut PHONPHO Sasiwimol WOOTTHIKANOKKHAN Siriporn PRAMRIT Somsak MANEEPONG Srisakul VORACHANTRA Suchada BOONLERTNIRUN Suchatvee SUWANSAWAT Sudathip CHANTORN Sudteerak SAIPLUEMCHIT Sukunya YAMPRACHA Suneeporn SUWANMANEEPONG Suneerat RUANGSOMBOON Suphachai AMKHA Surasak SAJJABUT Suriyasit SOMNUEK Sutichai SAMART Syed BILAL HUSSAIN Tanimnun JAENAKSORN Teerawat SARUTAYOPHAT Thamrong MEKHORA Thanapa CHETAWAN Thierry TRAN Thiwakorn AMPAPON Tippawan LIMUNGGURA Titi THONGKAMNGAM

July 1-3, 2015

Thailand Thailand Thailand Thailand Thailand Thailand Malaysia Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Pakistan Thailand Thailand Thailand Thailand France Thailand Thailand Thailand

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121. 122. 123. 124. 125. 126. 127. 128. 129. 130. 131. 132. 133. 134.

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Tupthai NORSUWAN Ubon TANGKAWANIT Vanapron SAE-ANG Wachiraporn PEWLONG Wallop LOMLIM Wanida DUANGKONGSAN Wannasiri WANNARAT Waraporn CHOUYCHAI Warin PIMPA Warong NAIVINIT Wirote KLIBSUWAN Wisara THUTO Withan TACHAKOMOL Worawan PANPIPAT

Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand Thailand

July 1-3, 2015

2 International Symposium on Agricultural Technology (ISAT2015)

Authors Index

ADICHANUND, S............................................. 135 ALI, Z. ................................................................. 87 AMIR MANZOOR, S. ....................................... 114 AMKHA, S. ................................................. 60, 112 AMPAPON, T. ..................................................... 70 ANANTAWAT, V. .............................................. 71 ANGKANANON, W. .......................................... 71 AREEWONG, J. ................................................... 126 ARRUEJO, S. J. M............................................... 88 AUE-UMNEOY, D. ............................................. 63 BABJI, A.S............................................................. 43 BANJONG, K. ..................................................... 72 BAOLEE, A. ...................................................... 115 BASKAR, M. ....................................................... 62 BHARTI, V. ......................................................... 51 BHAT, R. ............................................................. 39 BHENDARKAR, D.R.......................................... 86 BILAL HUSSAIN, S.................................... 87, 114 BOONLERTNIRUN, K. ................................ 96, 97 BOONLERTNIRUN, S. ................................. 96, 97 BOONPLANG, N. ...................................... 117,121 BUSSABA, N. ..................................................... 50 CHAIJAN, M. ............................ 131, 132, 133, 141 CHALERMWANICHWONG, J. ....................... 139 CHANGSAWAKE, K. ................................. 54, 122 CHANTORN, S. ................................................ 152 CHAOSAP, C. ................................................... 138 CHETAWAN, T. ............................................... 137 CHIANGKHAM, W. ......................................... 152 CHOOKAEW, S. ............................... 100, 101, 102 CHOUYCHAI, W. ............................................... 91 CHUANOI, S. .................................................... 151 CHULILUNG, P. ....................................... 148, 149 CHUTIPAIJIT, S.................................................. 93 CROSER, J........................................................... 42 DAVIES, S. .......................................................... 64 DIXIT, A. ............................................................. 79 DONRODPRI, J. .................................................. 91 DUANGDEE, S. .................................................. 67 DUANGPAKDEE, K. ........................................ 134 DÜNDAR, A. ..................................................... 153 EAMSIRI, J. ....................................... 100, 101, 102 GANDAHI, A. ..................................................... 59 GANDAHI, R. ..................................................... 59 GANMANEE, M. ............................ 63, 65, 66, 129 HAMAD, A. ......................................................... 74 HAMANO, K. ...................................................... 63 HANBOONSONG, Y. ....................................... 127 HASSAN, F. ...................................................... 114 HUU NGUYEN, H. ............................................. 61 IMRAN UMAR, M. ........................................... 114 INSUNG, A. ......................................................... 126 ISHII, H................................................................ 45 JAENAKSORN, T. ........... 77, 78, 80, 81, 82, 83, 84 JAIKENGKAJ, K. ................................................ 83 JAMIN PRIYO MINARJO, H. ............................ 58 JAMJANYA, T. ................................................. 145 JAMPATHONG, N. ............................................. 92 JINTRAWET, A. ................................................. 49 JIRAJAROENRAT, K. .............................. 139, 140 JITTAMNAN, N. ............................................... 136 JOMPUK, C. ........................................................ 96

July 1-3, 2015

KAEWSONGSANG, K. .................................... 115 KAMSAN, P. ..................................................... 107 KANTAVICHAI, R. .......................................... 129 KANYAWONGHA, P. .............................. 116, 117 KHARE, C.P. ........................................... 79, 85, 86 KHLAIHIRAN, N. ............................................. 152 KHLIBSUWAN, W. .................................. 127, 145 KHONNALAO, Y. ............................................ 144 KHURNPOON, L. ............................................. 109 KITPRECHAWANICH, W. ................................ 56 KITTIWONGWATTANA, C. ........................... 123 KLOMKLING, S. ................................................ 63 KOCHARIN, P. ................................................... 65 KONGKARN, N. ............................................... 122 KONGTRAGOUL, P. ...................... 76, 77, 84, 126 KOOHAKAN, P. ................................................. 83 KOTASTHANE, A.S. .......................................... 85 KOTESTHANE, A............................................... 79 KUMAR RAINA, A. ........................................... 51 LAOPHROM, N. ............................................... 141 LAOSINWATTANA, C. 52, 54, 104, 106, 107, 108 LERTKRASEMPON, C. .................................... 149 LERTPANICH, K. ............................................. 130 LIMSUPAVANICH, R. ..................................... 138 LIMUNGGURA, T. ................................... 146, 149 LOKAEWMANEE, K.......................................... 67 MAKLAI, A. ........................................................ 92 MALA, T. .................................................... 60, 112 MANEEPONG, S. ....................................... 61, 111 MANKEB, P. ..................................... 146, 148, 149 MANURAKCHINAKORN, S. .................. 103, 105 MARONH, C. ...................................................... 49 MATONG, P. ..................................................... 148 MEEANAN, C. .................................................... 63 MEEPOKA, C. ............................................... 65, 66 MEKHORA, T. .................................................. 129 MERRIFIELD, D. ................................................ 64 MISHRA, A. ........................................................ 53 MUHAMMAD ZAHID KHAN, D. .................. 114 NAIVINIT, W. ................................................... 150 NAWAZ KHAN, H.............................................. 87 NAZ MIRZA, S. ................................................ 114 NETSAWANG, P. ............................................. 108 NGAMSANGA, T. ............................................ 139 NGUYEN THIEN TRUONG, G. ......................... 68 NIAMNUY, C. ..................................................... 73 NISOA, M. ........................................................... 71 NITHISANTAWAKHUPT, J. ........................... 143 NITMEE, P. ....................................................... 112 NOKKOUL, R. .................................................... 94 NORSUWAN, T. ................................................. 49 NOUMAN, W. .................................................. 114 NUR‘ ALIAH, D. ................................................... 43 NURSYAMSI, D. ................................................ 58 NURUL NADIA, M................................................ 43 NUUDOM, F. .................................................... 105 OKUMUŞ, V. .................................................... 153 ONDEE, A. ........................................................ 103 OSOTHONGS, M. ............................................. 140 ÖZDEMİR, S. .................................................... 153 PANCHAN, N...................................................... 73 PANHWAR, K. .................................................... 59

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Authors Index

PANKAEW, Y. .................................................... 56 PANNANGPETCH, K. ...................................... 127 PANPIPAT, W. .......................... 131, 132, 133, 141 PARINTHAWONG, N. ......................... 80, 81, 125 PATRARASRIPONG, K. .................................. 130 PERMHIRUN, T. ................................................. 92 PEWLONG ,W. ................................. 100, 101, 102 PHAKAMAS, N. ........................................... 92, 93 PHESATCHA, K. .......................................... 69, 70 PHONCHAROEN, P. .......................................... 50 PHURUEN, N. ................................................... 104 PILASOMBUT, K. ...................... 54, 122, 137, 142 PIMPA, C. ...................................................... 98, 99 PIMPA, W. ..................................................... 98, 99 PINPHOO, P. ....................................................... 63 POEAIM, A. ........................................................ 95 POEAIM, S. ....................................................... 124 PONGJAROENKIT, S. ........................................ 95 PONGPOUN, S. ................................................. 118 PONGTHONGKAM, P........................................ 95 PORAHA, R......................................................... 95 PORNRATANACHOTSAKUL, M. .................. 129 PRADIPTA, A. .................................................... 58 PRADUBYAT, M. ............................................... 81 PRAKASH, K. ...................................................... 57 PRAKHONGSIL, P. .................................. 100, 101 PRAMRIT, S. ..................................................... 125 PREMPRAMOTE, J. ........................................... 66 PUMNUAN, J....................................................... 126 PUSPAWININGTYAS, E. ................................... 74 PUSPAWININGTYAS, G. .................................. 74 RATANANIKOM, K. ........................................ 144 RAZZAGHI, M. ................................................... 75 RELOX, N. A. ...................................................... 88 REVATHI, J......................................................... 62 ROENGANAN, N. ............................................. 118 RUANGSOMBOON, S...................................... 128 RUEN-NGAM, D. ............................................... 94 RUNGCHAROENTHONG, P. .................... 60, 112 SADEGHIPOUR, O. .......................................... 110 SAE-ANG, V. ...................................................... 94 SAHA, A. ............................................................. 51 SAHILAH, A.M. .................................................... 43 SAIPLUEMCHIT, S. ........................................... 52 SAJJABUT, S. ................................... 100, 101, 102 SAKDAPECHSIRI, C. ....................................... 140 SAMAKRAMAN, S. ........................................... 66 SAMART, S. ........................................................ 93 SANPRASERT, R.............................................. 150 SARUTAYOPHAT, T. ........................................ 50 SENASING, V. .................................................. 145 SERTSUNGNONE, K. ........................................ 92 SETHPAKDEE, R. ............................................ 115 SHARMA, D. ....................................................... 79 SINGH, J. ............................................................. 79 SIRIVEJABANDHU, K. .................................... 109 SITTHIGRIPONG, R. ........................................ 138 SIVAPIRUNTHEP, P. ....................................... 136 SOMNUEK, S. ............................................... 77, 84

Page 160

SOMTRATOON, K. ............................................ 91 SONGKRAM, N. ............................................... 147 SONGPHAT KAEW, J. ............................................. 63 SORAPUKDEE, S. .................................... 137, 142 SOYTONG, M. .................................................. 124 SRIKIJKASEMWAT, K. ................................... 139 SRINAMNGOEN, P. ................................. 134, 135 SRIPRACHOTE, A. ................................... 116, 117 SRIROD, S. ........................................................ 144 SUEBNUKARN, P. ........................................... 150 SUKCHAI, H. ...................................................... 63 SUKKAEW, E. .................................................... 60 SUKSUPATH, K. .............................................. 143 SUNDHAR SINGH, S.D. ..................................... 57 SUPANSOMPORN, S. ........................................ 56 SURANINPONG, P. ............................................ 61 SUVANNASARA, R............................................ 97 SUWANMANEEPONG, S. ............................... 148 SUWANPANYA, N........................................... 144 SWAMY, S. L. ..................................................... 53 SWAMY, S.L. ...................................................... 86 TAJASRI, N. ...................................................... 138 TALUBNAK, C. .................................................. 80 TANGKAWANIT, U. ........................................ 145 TANGWATCHARIN, P. ........................... 122, 143 TAWAI, C............................................................ 94 TAWINTEUNG, N. ................................... 113, 120 TEAMKAO, P.................................................... 118 TEERARAK, M. ..... 54, 55, 104, 106, 107, 108, 122 THAMNIYOM, B. ............................................. 151 THONGKAMNGAM, T. ............................... 78, 82 THONGSAMUI, A. ........................................... 129 THUTO, W. ......................................................... 72 TIWARI, P.K. ...................................................... 85 TRAN, T. ..................................................... 46, 140 TRUKTRONG, S. .............................................. 141 TSUTSUI, I. ......................................................... 63 TUMTONG, W. ................................................. 133 UESAKULRUNGRUENG, C. ........................... 142 VIJAYAKUMAR, R.M. ....................................... 57 VIRIYAEKKUL, P. ............................................. 76 WANAPAT, M. ................................. 37, 68, 69, 70 WANNARAT, W. ................................................ 56 WATHAKIATTIKUL, P. .................................. 119 WATTANARSAKIT, P. ...................................... 71 WICHITTRAKARN, P. ..................... 106, 107, 108 WILLIAMS, G. A. ............................................... 65 WISEMAN, J. ...................................................... 41 WIYACHAI, T. .................................................. 150 WONGSIRIDETCHAI, C. ................................. 152 WONGWEAN, P. ................................................ 56 WOOTTHIKANOKKHAN, S. .......................... 151 YAMPRACHA, S. ............................................. 119 YOMLA, R. ......................................................... 64 YONSAWAD, N.................................................. 55 ZUBAIR, M. ...................................................... 114

July 1-3, 2015