BIOLOGY
L A B O R AT O R Y E X P E R I M E N T S
About Phywe
Founded in Gรถttingen, Germany in 1913 by Dr. Gotthelf Leimbach, Phywe Systeme GmbH & Co. KG quickly advanced to one of the leading manufacturers of scientific equipment. Over this period of more than 90 years Phywe has been putting quality and innovation into its products as a fundamental requirement. As a well known international supplier in the fields of science and engineering we have made a significant impact on the market through high quality equipment. Phywe products are made in Germany and in use throughout the world in the fields of education and research, from primary schools right through to university level. Up-to-date educational systems, planning and commissioning of scientific and engineering laboratories to meet specific requirements are our daily business. As a supplier of complete, fully developed and established systems, Phywe provides teaching and learning systems for students as well as teacher demonstration experiments. The system ranges from simple, easy to operate equipment intended for student use up to coverage of highly sophisticated and specialised university equipment demands. Phywe Systeme GmbH & Co. KG has achieved a very high standard based on research and technology and through exchange of experiences with universities and high schools as well as with professors and teachers. As experienced and competent manufacturer, we would gladly assist you in the selection of the "right" experiments for your particular curricula.
Contents
Contents Page LEB 01 LEB 01.01
Nervous System
Page LEB 09
Derivation of nerve and muscle potentials by mechanical simuIation at the rear end of an earthworm
8
LEB 01.02
Derivation of nerve and muscIe potentials by mechanicaI stimulation at the front end of an earthworm
9
LEB 01.03
Derivation of nerve potentials after eIectrical stimulation of an anaesthetized earthworm
10
Respiration
LEB 09.01
Volumetric measurement of breathing in small animals
50
LEB 09.02
Measurement of the human respiratory rate
51
LEB 10
Ecology and environment
LEB 10.01
Comparison of the heat capacities of water and land
54
LEB 10.02
The Bergmann rule: Heat loss in dependence on body surface area and volume
55
LEB 10.03
The insulating effect of body coverage
56
LEB 01.04
Model experiment illustrating the deveIopment of resting potential
LEB 01.05
Neurosimulator: Membrane time constant and low-pass filtering
12
LEB 10.04
The daily course of luminosity
57
Neurosimulator: How an exciting synapse functions
13
LEB 10.05
The conductivity of various water samples
58
LEB 10.06
The pH of various water samples
59
LEB 10.07
The origin of acid rain
60
LEB 10.08
The twenty-four hour rhythm of an aquatic plant
61
LEB 01.06 LEB 02
11
Heart and Circulatory System
LEB 02.01
Human electrocardiography (ECG)
16
LEB 02.02
Phonocardiography: Cardiac and vascular sonic measurement (PCG)
17
LEB 11
LEB 02.03
Blood pressure measurement
18
LEB 11.01
LEB 02.04
Changes in the blood flow during smoking
19
LEB 03
Musculature
64
LEB 11.02
Photosynthesis (measurement of O2 pressure)
65
LEB 11.03
Transpiration of leaves
66
LEB 11.04
Glycolysis (measurement of pressure)
67
Glycolysis (measurement of temperature)
68
LEB 03.01
EIectromyography (EMG) on the upper arm
22
LEB 11.05
LEB 03.02
Muscle stretch reflex and determination of conducting veIocity
23
LEB 12
LEB 04
Auditory Sense
Plant physiology Photosynthesis (bubble counting method)
Biochemistry
LEB 12.01
Determination of the isoelectric point of an amino acid (glycine)
70
LEB 12.02
The ionic permeability of the cell membrane
71
LEB 04.01
Human merging frequency and upper hearing threshold
26
LEB 04.02
Hearing threshold and frequency differentiating threshold in humans
LEB 12.03
Determination of the Michaelis constant
72
27
LEB 12.04
Substrate inhibition of enzymes
73
Acoustic orientation in space
28
LEB 12.05
Enzyme inhibition (poisoning of enzymes)
74
LEB 12.06
The enzymatic activity of catalase
75
LEB 04.03 LEB 05
Gravitational Sense
LEB 05.01
Phototropism and geotropism
30
LEB 05.02
The effect of gravity and centrifugal force on plants
31
LEB 06
Temperature Sense
LEB 06.01
Effect of soil temperature on the germination and growth of higher plants
34
LEB 06.02
Preferential temperature in insects
35
LEB 06.03
Regulation of human body temperature
36
LEB 07
Visual Sense
LEB 07.01
Optomotor response in insects
38
LEB 07.02
Determination of the human visual field
39
LEB 07.03
Time resolving capability of the human eye
40
LEB 07.04
Test of human reaction capacity
41
LEB 07.05
Human electrooculography (EOG)
42
LEB 07.06
Additive and subtractive colour mixing and colour maskings 43
LEB 08 LEB 08.01
Behaviour Wall contact searching in mice
46
LEB 08.02
Learning and marking behaviour of mice
47
LEB 08.03
Learning performance of humans
48
1
Phywe in the University City of Göttingen –
Natural sciences have a longstanding tradition in Göttingen. More than 40 Nobel prize winners coming from all sorts of scientific disciplines and numerous university institutes successfully conduct research in practically all areas of science.
The following research institutions and university institutes are located in Göttingen: Academy of Science, several Max-Planck institutes, the German Primate Centre, the Centre of Molecular Physiology of the Brain, the Centre of Molecular Life Science – to name just a few.
We are in contact with these institutions and exchange our views with them to ensure that the latest trends and scientific innovations are always reflected in the product range of Phywe Systeme GmbH & Co. KG.
2
A Center of Natural Sciences in Germany
GÖTTINGEN is a city of teaching and research. Scientific equipment, teaching equipment and laboratory installations developed and produced in this city are famous throughout the world. Göttingen would not be what it is without its university. “Georgia Augusta” was founded in 1734 and by 1777 it was Germany‘s largest university, with 700 students. It still is one of the leading universities in Germany, with 14 faculties, significant scientific facilities and more than 30,000 students. The gracious Goose Girl (“Gänseliesel”) on the market place fountain is the most kissed girl in Germany. Why? Because every newly graduated doctor must kiss the cold beauty on her bronze mouth. That is Göttingen tradition. Doctor’s kiss for the Goose Girl
3
Laboratory Experiments Biology
LEB
The experiments in the PHYWE Publication Series “Laboratory Experiments Biology” are intended for the heads of biology laboratory courses at universities, colleges and similar institutions and also for advanced courses in high schools. All experiments are uniformly built-up and contain references such as Related topics and Principle and task to introduce the subject. O pressure) (measurement of 2 Photosynthesis
02074.00 , 160 x 130 mm 06759.93 Lab-lifting apparatus V/120 W, w refl. 06751.00 Filament lamp, 220 mains connection Lamp socket E 27, DURAN 36008.00 form, tall ml, 1000 39258.01 Glass beaker, 1 hole mm, 32/26 = d 39282.00 Rubber stopper, 1m mm, 6 = d , 4.00 tubing mm, PN29 3629 g pho- Rubber Principle and task n pressure durin = 30 mm, l = 200 increase in oxyge To Test tube, d dark. the in ht, To compare the and tubes, straig 36701.65 light, green light pressure Glass the pcs of 10 tosynthesis in white nces 40485.01 80 mm, d = 7 mm, ns for the differe mm 3 = d mm, discuss the reaso 45990.93 Glass rod, l = 200 RS232 curve rise 51, 200 g/0.01 g, 33393.00 the Balance SAS al steel procedure using 48159.04 Microspoon, speci the experimental ing bottle, 25 ml Equipment (for 30151.25 Glycerol in a dropp g 250 nate, 12150.00* 1 carbo Basic-Unit) 48498.04 Sodium hydrogen g 12151.99* 1 25 Cobra3 Basic Unit 48376.04 Tartrazine (E 102), V g 25 14510.61* 1 Power supply, 12 131), Patent blue V (E - Pressure 14602.00* 1 Software Cobra3 Aluminium foil 12103.00* 1 RS232 data cable Water-weed ® le, pressure 02043.10* 1 Measuring modu PC, Windows small case 1 02001.00 Support clamp for le 2 Basic-Unit) Support base, variab steel 02032.00 Set-up (using the Fig. 1. 500 mm, stainless 1 02043.00 ment as shown in Support rod, l = — Set up the equip 1 37715.00 Bosshead Universal clamp the Basic-Unit using p set-u tal Fig. 1: Experimen
ure Related topics r of the light, press intensity and colou Photosynthesis, measurement
Results and evaluation states full theory involved and shows graphical and numerical experimental results including error calcules.
LEB 11.2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
LEB 11.2
Photosynthesis (measurement of O2 pressure)
— Fasten the support clamp for a small case to the back of the Cobra3 Basic Unit — Connect the basic unit to the computer with the data cable — Draw the support base about 30 cm apart and hold it so with the support rod — Insert the lamp in a hole in one half of the base — Fix both the Cobra3 Basic Unit with plugged-in pressure module and the test tube on the other half — Connect the glass tube in the rubber stopper to the pressure module with the shortest possible length of rubber tubing — Fill the beaker with water and place it between the lamp and the test tube to act as a heat filter
Fig. 2: Measurement parameters (using the Basic-Unit)
Results and evaluation — A distinct rise in the pressure curve is shown in the first experiment (Fig. 3) with white light — The pressure curve rises less steeply in the second experiment (Fig. 4) with green light — No rise in the pressure curve is to be seen in the third Photosyn experiment (Fig. 5) in the dark thesis (m — Photosynthesis results in the production of O2, which easureme nt of O causes a rise in the curve. NaHCO3 is used — pressure The 2 pressure) here as CO2 provideran green solution ab sorbs the d blue-v iolet ligh gre ate st t result is according required for photo part of the red ly less O synthesis only a slig . pro ht Th rise du 2 e ction and — No ph in the pre therefore otosyn ssure there is the thesis takes pla curve ce in n no — The water-filled rise at all in the pre the dark, so tha t ssure cur beaker Fig. 4: Result with green radlight iated out ve by the lam serves to absorb the warm p th Note This exp eriment can also held me be carrie asu d out usi sensor 07 ring instrument ng the ha pre 13 nd07157.01 6.01, software pre ssure 07136.00, pressure and boss ssure 14 41 the entrie he s in the eq ad 02043.00 (Fi 6.61, data cable g. 6). In asterisk uip ment list are not req this case, which are uired. marked wit h an Fig. 5: Re sult in the dark Fig. 6: Ex perimental set-up usi ng the ha nd-held me asuring ins trument pressure
Fig. 3: Result with white light en • D-37070 Götting SYSTEME GMBH Biology • © PHYWE tory Experiments publication • Labora Phywe Series of
Picture and Equipment List guarantee time-saving and easy conducting of the experiment.
13956
113
114
13956
Procedure (using the Basic-Unit) — Call up the COBRA3 MEASURE programme in Windows — Enter PRESSURE as measuring instrument — Set the measurement parameters (see Fig. 2) and go to measurement with CONTINUE — Cut off a stem from the water-weed and place it in the test tube with the cut end upwards — Prepare 300 ml of 3% NaHCO3 solution (9 g to 300 g distilled water) and fill the test tube up with it to just under the rim — Briefly remove the tubing from the pressure module nipple, close the test tube with the rubber stopper so firmly that it does not leak, then re-connect the tubing to the pressure module. Caution! Do not allow any liquid to enter the pressure module! — Switch on the lamp and start measurement — For the second experiment, use the dyes to prepare a green solution (one spatula tip each of the yellow and the blue dye made up to 1000 ml with water) and fill the beaker with it. Prior to measurement, remove the stopper to allow the gas which has formed to escape — For the third experiment, wrap the test tube with aluminium foil so that no light at all is incident on the plant. Prior to measurement, remove the stopper to allow the gas which has formed to escape
LEB 11.2
Phywe Series of publication • Laboratory Experiments Biology • © PHYWE SYSTEME GMBH • D-37070 Göttingen
Example for measurement parameters for easy and safe experimentation.
Phywe Ser ies of pub lication • Laboratory Experim ents Biol ogy • © PHYWE SYSTEM E GMBH • D-3707 0 Götting en 13956
115
Experimental literature Laboratory Experiments Biology
4 Laboratory Experiments, Biology
Long Version
No. 16506.02
CD
No. 16502.42
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments Biology
LEB
The present volume which has been developed by PHYWE, complements the previously existing collection of about 50 experiments in twelve chapters as the following comprehensive Table of Contents shows. In this brochure we present the experiments in short form. The experiments can be ordered or offered completely or partially, if desired, in accordance with the Comprehensive Equipment Lists. On request, we will gladly send you detailed experimental descriptions.
You can order the experiments as follows: What you need:
Didactically adapted descriptions of experiments – easy, direct preparation by the students is possible
Cobra3 BASIC-UNIT, USB
12150.50
Power supply, 12 V
12151.99
1 1
Cobra3 Universal Recorder software
14504.61
1
Bio-amplifier
65961.93
1
Stimuli generator
65962.93
1
Earthworm experiment chamber
65981.20
1
Connecting cord, 32 A, l = 25 cm, red
07360.01
1
Connecting cord, 32 A, l = 25 cm, blue
07360.04
1
Connecting cord, 32 A, l = 25 cm, black
07360.05
2
Connecting cord, 32 A, l = 50 cm, red
07361.01
1
Connecting cord, 32 A, l = 50 cm, blue
07361.04
1
Crocodile clip, insulated, black
07276.05
1
Petri dish, d = 100 mm
64705.00
1
Spoon with spatula end, l = 150 mm, steel, wide
33398.00
1
Balance SAS 51, 200 g/0.01 g, RS232
45990.93
1
Graduated cylinder, 100 ml
36629.00
1
Quantity Order No.
Aluminium foil Earthworms Chloretone as anaesthetic (pharmacy or dentist: 1,1,1-trichloro-2-methyl-2-propanol)
Please specify this Order No. if you would like to order the complete experiment.
PC, Windows® 95 or higher
Derivation of nerve potentials after electrical stimulation of an anaesthetized earthworm P4010311
Comprehensive experiments – cover the entire range of classical and modern biology
Complete equipment offering modular experimental set-up – multiple use of individual devices, cost effective and flexible
Excellent measurement accuracy – results agree with theory
Developed and proven by practicians – unproblematical and reliable performance
Computer-assisted experiments – simple, rapid assessment of the results
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 5
LEB_1_2008
15.04.2008
13:58 Uhr
Seite 6
– catalogues, brochures and more… PHYSICS – CHEMISTRY – BIOLOGY The comprehensive catalogue for physics, chemistry and biology. Additionally you can find a large number of laboratory materials and an insight in our particularly successful teaching systems TESS, Cobra3 and Natural Sciences on the board. Available in German, English and Spanish.
Laboratory Experiments The experiments in the Phywe publication series “Laboratory Experiments” are intended for the heads of laboratories, colleges of advanced technology, technical colleges and similar institutions and also for advanced courses in high schools. Laboratory Experiments is also available on CD-ROM (Order No. 16502.42). Available in English. For the student system “Advanced Optics and Laser Physics” a special brochure is available in English. Special brochures Additionally there are special brochures for our particularly successful teaching systems TESS (available in German, English, French and Spanish), Cobra3 (available in German and English) and Natural Sciences on the board (available in German and English).
6
1
Nervous System Contents LEB 01.01
Derivation of nerve and muscle potentials by mechanical simuIation at the rear end of an earthworm
LEB 01.02
Derivation of nerve and muscIe potentials by mechanicaI stimulation at the front end of an earthworm
LEB 01.03
Derivation of nerve potentials after eIectrical stimulation of an anaesthetized earthworm
LEB 01.04
Model experiment illustrating the deveIopment of resting potential
LEB 01.05
Neurosimulator: Membrane time constant and low-pass filtering
LEB 01.06
Neurosimulator: How an exciting synapse functions
Nervous System
LEB 01
01.01 Derivation of nerve and muscle potentials by mechanical stimulation at the rear end of an earthworm What you can learn about Nerve and muscle potentials Mechanical stimulation Biphasic action potential Frequency modulation Median and lateral giant nerve fibres Conduction velocity
Principle and tasks: To work on the following themes by measuring nerve and muscle potentials: ● The course of a biphasic action potential over time ● Estimation of the conduction velocity ● Coding of the stimulant intensity as frequency modulation
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Bio-amplifier
65961.93
1
Earthworm experiment chamber
65981.20
1
Stimulus bristle, triggering
65981.21
1
Connecting cord, 32 A, l = 250 mm, red
07360.01
2
Connecting cord, 32 A, l = 250 mm, blue
07360.04
2
Connecting cord, 32 A, l = 250 mm, black
07360.05
2
Crocodile clip, insulated, black, 10 pieces
07276.15
1
Adapter BNC plug/4 mm sockets
07542.26
1
Aluminium foil Earthworms
Result with weak stimulation
PC, Windows® 95 or higher
Derivation of nerve and muscle potentials by mechanical stimulation at the rear end of an earthworm P4010111
Result with moderate stimulation
8 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Nervous System
LEB 01
Derivation of nerve and muscle potentials by mechanical stimulation at the front end of an earthworm 01.02 What you can learn about Nerve and muscle potentials Positive feedback Synaptic depression Synaptic facilitation Conduction velocity in median and lateral giant nerve fibres
Principle and tasks: To work on the following themes by measuring nerve and muscle potentials: ● The difference in sensitivity of the front and rear ends ● The facilitation effect ● Conduction velocity and fibre diameter ● Synaptic depression
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Bio-amplifier
65961.93
1
Earthworm experiment chamber
65981.20
1
Stimulus bristle, triggering
65981.21
1
Connecting cord, 32 A, l = 250 mm, red
07360.01
2
Connecting cord, 32 A, l = 250 mm, blue
07360.04
2
Connecting cord, 32 A, l = 250 mm, black
07360.05
2
Crocodile clip, insulated, black, 10 pieces
07276.15
1
Adapter BNC plug/4 mm sockets
07542.26
1
Aluminium foil Result with weak stimulation
Earthworms PC, Windows® 95 or higher
Derivation of nerve and muscle potentials by mechanical stimulation at the front end of an earthworm P4010211
Result with infrequent strong stimulation
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 9
Nervous System
LEB 01
01.03 Derivation of nerve potentials after electrical stimulation of an anaesthetized earthworm What you can learn about Nerve and muscle potentials Electrical stimulation Anaesthetization of muscles Electrical resistance of nerve fibres Double pulse stimulation Refractory period
Principle and tasks: To work on the following themes by measuring nerve and muscle potentials: ● The action of an anaesthetic ● The different conduction velocities of median and lateral giant fibres ● Refractory period of the median giant fibre
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Bio-amplifier
65961.93
1
Stimuli generator
65962.93
1
Earthworm experiment chamber
65981.20
1
Connecting cord, 32 A, l = 250 mm, red
07360.01
1
Connecting cord, 32 A, l = 250 mm, blue
07360.04
1
Connecting cord, 32 A, l = 250 mm, black
07360.05
2
Connecting cord, 32 A, l = 500 mm, red
07361.01
1
Connecting cord, 32 A, l = 500 mm, blue
07361.04
1
Crocodile clip, insulated, black, 10 pieces
07276.15
1
Petri dish, d = 100 mm
64705.00
1
Spoon with spatula end, l = 150 mm, steel, wide
33398.00
1
Balance SAS 51, 200 g/0.01 g, RS232
45990.93
1
Graduated cylinder, 100 ml
36629.00
1
Result with a weak stimulus
Aluminium foil Earthworms Chloretone as anaesthetic (pharmacy or dentist: 1,1,1-trichloro-2-methyl-2-propanol) PC, Windows® 95 or higher
Derivation of nerve potentials after electrical stimulation of an anaesthetized earthworm
P4010311
Result with a double pulse 6 ms
10 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Nervous System
LEB 01
Model experiment illustrating the development of resting potential 01.04 What you can learn about Selective ion permeability of membranes Resting potential Diffusion potential Asymmetry potential Silver chloride electrodes Ion pump
Principle and tasks: The potential difference between two electrolyte solutions of different concentrations separated by a membrane (cellophane or cation permeable) is detected by two silver chloride electrodes and measured with a mV meter. The measured and calculated values are compared.
What you need: Ussing chamber
65977.00
1
Reference electrode, silver chloride
18475.00
2
mV/pH meter, hand held
07139.00
1
Adapter BNC plug / 4 mm sockets
07542.26
1
Temperature probe Pt 1000, glass
13702.01
1
Volumetric flask, 500 ml, IGJ 19/26
36551.00
1
Volumetric flask, 1000 ml, IGJ 24/29
36552.00
1
Graduated cylinder, 100 ml
36629.00
2
Cylinder, 200 x 40 mm, 180 ml
34213.00
1
Bottle, narrow neck, plastic, 500 ml
33906.00
4
Bottle, narrow neck, plastic, 1000 ml
33907.00
6
Spoon with spatula end, 150 mm, steel
33398.00
1
Cellophane, 200 x 200 mm, 10 sheets
32987.00
1
Membrane, permeable for cations, 5 pieces
31504.02
1
Potassium chloride, 250 g
30098.25
1
Sodium chloride, 250 g
30155.25
1
Hydrochloric acid, 1 l, 0.1 mol/l
48452.70
1
Distilled water, 5 I
31246.81
2
Balance SAS 51, 200 g / 0.01 g, RS 232
45990.93
1
Model experiment illustrating the development of resting potential
P4010450
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
Laboratory Experiments, Biology 11
Nervous System
LEB 01
01.05 Neurosimulator: Membrane time constant and low-pass filtering What you can learn about Time constant of nerve membrane Intracellular potential Low-pass characteristic
Principle and tasks: To use a nerve function model to work on the following themes: ● The time constant of the nerve membrane and intracellular potential ● The low-pass characteristic of the nerve membrane
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Neurosimulator
65963.00
1
Neurosimulator, power supply
65963.93
1
Connecting cord, 32 A, l = 500 mm, yellow
07361.02
1
PC, Windows® 95 or higher
Result of a single stimulation
Neurosimulator: Membrane time constant and low-pass filtering
12 Laboratory Experiments, Biology
P4010511
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Nervous System
LEB 01
Neurosimulator: How an exciting synapse functions 01.06 What you can learn about Action potentials Intracellular potential Excitatory postsynaptic potential (EPSP) Nodes of Ranvier Minimum stimulus intensity Frequency modulation
Principle and tasks: To use a nerve function model to work on the following themes: ● The origin of action potentials ● The transformation of stimulation intensity to action potential frequency
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Neurosimulator
65963.00
1
Neurosimulator, power supply
65963.93
1
Connecting cord, 32 A, l = 500 mm, yellow
07361.02
1
PC, Windows® 95 or higher
Result of a strong stimulation
Neurosimulator: How an exciting synapse functions
P4010611
Result of a weak stimulation
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 13
14
2
Heart and Circulatory System Contents LEB 02.01
Human electrocardiography (ECG)
LEB 02.02
Phonocardiography: Cardiac and vascular sonic measurement (PCG)
LEB 02.03
Blood pressure measurement
LEB 02.04
Changes in the blood flow during smoking
Heart and Circulatory System
LEB 02
02.01 Human electrocardiography (ECG) What you can learn about Electrophysiology Electrocardiogram according to Einthoven II Heart rate Quiet and strained heart ECG segments Atria Ventricles AV nodes
Principle and tasks: To record an electrocardiogram (ECG) between the left leg and the right and left arm (lead II according to Einthoven). To relate the ECG segments to the course of heart contraction (P wave, P-Q segment, QRS complex, T wave)
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Bio-amplifier
65961.93
1
Electrode collecting cable
65981.03
1
ECG electrodes, 3 pieces
65981.01
1
Potassium chloride, 250 g
30098.25
1
Connection cord, 32 A, l = 250 mm, red
07360.01
1
Connection cord, 32 A, l = 250 mm, blue
07360.04
1
Spoon with spatula end, l = 150 mm, steel, wide
33398.00
1
Balance SAS 51, 200 g/0.01 g, RS232
45990.93
1
Graduated cylinder, 100 ml
36629.00
1
PC, Windows® 95 or higher Tempo/Kleenex Tissues Circuit diagram
Human electrocardiography (ECG) P4020111
Typical measuring results 16 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Heart and Circulatory System
LEB 02
Phonocardiography: Cardiac and vascular sonic measurement (PCG) 02.02 What you can learn about Pulse Throat and chest sonic measurement Quiet and strained heart Contraction tune Systole Flapping sound Diastole
Principle and tasks: Cardiac and vascular sonic measurement at different locations of the circulatory system. Measurement of the pulse rate at different levels of athletic loading.
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Acustic measuring probe
03544.00
1
PC, Windows® 95 or higher
Typical vascular phonometric measurement
Phonocardiography: Cardiac and vascular sonic measurement (PCG)
P4020211
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 17
Heart and Circulatory System
LEB 02
02.03 Blood pressure measurement What you can learn about Systolic blood pressure Diastolic blood pressure Measuring cuff Blood pulse waves
Principle and tasks: To prepare a plot of blood pressure measurement and to read the values of systolic and diastolic blood pressure.
What you need: Cobra3 BASIC-UNIT, USB
12150.50
Power supply, 12 V
12151.99
1 1
Software Cobra3 - Pressure
14510.61
1
Measuring module, pressure
12103.00
1
Blood pressure measurement set
64234.00
1
Y piece
47518.01
1
PC, Windows® 95 or higher
Blood pressure measurement P4020311 Typical result
18 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Heart and Circulatory System
LEB 02
Changes in the blood flow during smoking 02.04 What you can learn about Skin temperature Heavy and moderate smokers Occasional smokers Non-smokers
Principle and tasks: To prepare a curve showing the change in skin temperature during smoking. To discuss different curves depending on the smoking habits of the test person.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB
12150.50 * 1
Power supply, 12 V
12151.99
Software Cobra3 - Temperature
14503.61 * 1
1
Semiconductor temperature sensor -20...110°C
12120.00 * 1
Precision thermometer, –10...+100°C
47040.00
1
Cigarettes PC, Windows® 95 or higher
Changes in the blood flow during smoking with Cobra3 BASIC-UNIT
P4020411
Changes in the equipment required for use of the CHEM-UNIT: (Instead of * above-mentioned) Cobra3 CHEM-UNIT
12153.00
1
Data cable, plug/socket, 9 pole
14602.00
1
Software Cobra3 CHEM-UNIT
14520.61
1
Immersion probe NiCr-Ni
13615.03
1
Changes in the blood flow during smoking with Cobra3 CHEM-UNIT
P4020440
Typical result
Experimental set-up using the CHEM-UNIT (P4020440)
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 19
20
3
Musculature Contents LEB 03.01
EIectromyography (EMG) on the upper arm
LEB 03.02
Muscle stretch reflex and determination of conducting veIocity
Musculature
LEB 03
03.01 Electromyography (EMG) on the upper arm What you can learn about Electrophysiology Electromyogram Muscle contractions Biceps Muscle potentials Compound action potentials
Principle and tasks: To prepare an electromyogram (EMG) from a contracting or relaxing upper arm muscle (biceps) using surface electrodes. Measurement of the frequency and the amplitude of the EMG at maximum contraction.
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Bio-amplifier
65961.93
1
EMG electrodes
65981.02
1
Electrode collecting cable
65981.03
1
Electrode cream, tube
65981.05
1
Connecting cord, 32 A, l = 250 mm, red
07360.01
1
Connecting cord, 32 A, l = 250 mm, blue
07360.04
1
Roll of adhesive tape (e.g. Elastoplast) PC, Windows® 95 or higher Attaching the electrodes
Electromyography (EMG) on the upper arm P4030111
Typical result
22 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Musculature
LEB 03
Muscle stretch reflex and determination of conducting velocity 03.02 What you can learn about Electrophysiology Electromyogram Muscle stretch reflex Achilles tendon Reflex latency Conduction velocity Jendrassik effect Facilitation
Principle and tasks: To trigger a stretch reflex in the lower leg musculature by tapping the Achilles tendon (Achilles tendon reflex). To record the compound action potential and determine the reflex latency and the conduction velocity.
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Bio-amplifier
65961.93
1
EMG electrodes
65981.02
1
Electrode collecting cable
65981.03
1
Electrode cream, tube
65981.05
1
Reflex hammer, triggering
65981.10
1
Connecting cord, 32 A, l = 250 mm, red
07360.01
1
Connecting cord, 32 A, l = 250 mm, blue
07360.04
1
Roll of adhesive tape (e.g. Elastoplast) PC, Windows® 95 or higher Attaching the electrodes
Muscle stretch reflex and determination of conducting velocity
P4030211
Typical result
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 23
24
4
Auditory Sense Contents LEB 04.01
Human merging frequency and upper hearing threshold
LEB 04.02
Hearing threshold and frequency differentiating threshold in humans
LEB 04.03
Acoustic orientation in space
Auditory Sense
LEB 04
04.01 Human merging frequency and upper hearing threshold What you can learn about Acoustic hearing thresholds Merging frequency Hearing range Sine wave generator
Principle and tasks: Determination of the merging frequency and upper acoustic threshold of test subjects of various ages.
What you need: Headphones, stereo
65974.00
1
Sine wave generator
65960.93
1
Human merging frequency and upper hearing threshold
P4040100
26 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
Auditory Sense
LEB 04
Hearing threshold and frequency differentiating threshold in humans 04.02 What you can learn about Hearing threshold curve Frequency differentiation thresholds Hearing range
Principle and tasks: 1. Determine the hearing threshold for a number of frequencies in the hearing range of humans and plot a hearing threshold curve. 2. Determine the frequency difference between two sounds of the same intensity which can just still be perceived as two different sounds (frequency differentiating threshold). Plot a curve of the frequency differentiating threshold.
What you need: Sine wave generator
65960.93
1
Headphone, stereo
65974.00
1
Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Shielded BNC cable, l = 30 cm
07542.10
1
Adapter BNC socket/4 mm plug pair
07542.27
2
PC, Windows® 95 or higher
Hearing threshold and frequency differentiating threshold in humans P4040211
Frequency differentiating threshold curve
Hearing threshold curve PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 27
Auditory Sense
LEB 04
04.03 Acoustic orientation in space What you can learn about Spatial orientation Artificial head Acoustic probes Threshold angle Travelling time difference
Principle and tasks: To localize a source of sound using an artificial head. To measure the time difference and the difference in intensity of the sound waves incident on each ear of the artificial head.
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 Universal Recorder
14504.61
1
Artificial head
65975.01
1
Measuring microphone with amplifier
03543.00
2
Support base, variable
02001.00
1
Stand tube
02060.00
1
Protractor scale with pointer
08218.00
1
Tuning fork, 440 Hz, on resonance box
03427.00
1
PC, Windows® 95 or higher
Result for 0 degrees
Acoustic orientation in space P4040311
Result for 20 degrees
28 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
5
Gravitational Sense Contents LEB 05.01
Phototropism and geotropism
LEB 05.02
The effect of gravity and centrifugal force on plants
Gravitational Sense
LEB 05
05.01 Phototropism and geotropism What you can learn about Photoperiodism Phototaxis Clinostat Negative and positive phototropic response Geotropism Geotaxis Statoliths
Principle and tasks: To test the reaction of shoots, leaves and roots of higher plants to light stimuli (photoperiodism) and to stimuli caused by the earth’s field of gravity (geotropism). To measure the alignment of shoot, leaf and root of bean seedlings which sprout in a vessel standing on the ground or Iying on its side. To eliminate the effect of light or gravity by rotation in a clinostat aligned in the vertical or horizontal position.
What you need: Clinostat with electromotor 230 V
65964.93
1
Plastic beaker, 400 ml
36014.01
2
Petri dish, d = 100 mm
64705.00
1
Support base, variable
02001.00
1
Right angle clamp (bosshead)
02043.00
1
Support rod, l = 250 mm, stainless
02031.00
1
Bean seedlings Potting mould Goniometer
Phototropism and geotropism P4050100
Examination of phototropism
30 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Gravitational Sense
LEB 05
The effect of gravity and centrifugal force on plants 05.02 What you can learn about Positive geotropism of roots Negative geotropism of shoots Centrifugal force Rotational speed Statoliths
Principle and tasks: To measure the alignment of shoots and roots under the effect of centrifugal forces which are less than, equal to or greater than gravity. To raise sunflower seedlings in small beakers in a rotating drum. To set different centrifugal forces by changing the speed of rotation of the drum motor.
What you need: Strobe drum
65976.00
1
Insertion piece for strobe drum
65976.10
1
Motor with disc holder
11614.00
1
Power supply 0...12 V / 2 A
13505.93
1
Connecting cord, 32 A, l = 750 mm, red
07362.01
1
Connecting cord, 32 A, l = 750 mm, blue
07362.04
1
Support base, variable
02001.00
1
Right-angle clamp (bosshead)
02043.00
1
Support rod, l = 500 mm, stainless steeI
02032.00
2
Support rod, l = 250 mm, stainless steel
02031.00
1
Glass beakers, tall, 50 ml
36001.00 10
Sunflowers seeds Potting mould Goniometer
The effect of gravity and centrifugal force on plants
P4050200
Root orientation
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
Laboratory Experiments, Biology 31
32
6
Temperature Sense Contents LEB 06.01
Effect of soil temperature on the germination and growth of higher plants
LEB 06.02
Prefential temperature in insects
LEB 06.03
Regulation of human body temperature
Temperature Sense
LEB 06
06.01 Effect of soil temperature on the germination and growth of higher plants What you can learn about Plant germination Temperature optimum/ maximum/minimum Soil temperature Temperature preferendum
Principle and tasks: To investigate the effect of soil temperature on the germination of garden cress (Lepidium sativum). To determine the most favourable value for the germination and growth of cress using a temperature organ.
What you need: Temperature organ
65983.93
1
Immersion thermostat C10
08492.93
1
Accessory set for thermostat
08492.01
1
Bath for thermostat
08487.02
1
Rubber tubing, di = 8 mm
39283.00
2
Lab. thermometer, -10°C...+50°C
38055.00
6
Petri dish, d = 100 mm
64705.00
5
Garden soil Cress seed (Lepidium sativum)
Effect of soil temperature on the germination and growth of higher plants
P4060100
Optimum range for germination and growth of cress (to be completed)
34 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Temperature Sense
LEB 06
Preferential temperature in insects 06.02 What you can learn about Temperature optimum Poikilothermic animals Thermotaxis Phototaxis Thigmotaxis Torpor Environmental requirements Geographical separation
Principle and tasks: To test the temperature demands of poikilothermic animals. Using their thermoreceptors the animals recognize the temperature which suits them, gather in an area at the corresponding temperature (thermotaxis) and thus show their preferred temperature. To produce a temperature gradient between approximately 45°C and 10°C using a ring shaped temperature organ. To record the positions of the individual animals.
What you need: Temperature organ
65983.93
1
Immersion thermostat C10
08492.93
1
Accessory set for thermostat
08492.01
1
Bath for thermostat
08487.02
1
Lab. thermometer, -10°C...+50°C
38055.00
6
Rubber tubing, di = 8 mm
39283.00
2
Sea sand, purified, 1 kg
30220.67
1
10-20 insects of different species Marking colour (e.g. nail varnish)
Preferential temperature in insects P4060200
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 35
Temperature Sense
LEB 06
06.03 Regulation of human body temperature What you can learn about Body temperature regulation Radiation Evaporation Skin temperature Heating/cooling effects
Principle and tasks: To prepare curves demonstrating the regulation of body temperature. To discuss different curves depending on the conditions at the hand of the test person.
What you need: Cobra3 BASIC-UNIT, USB
12150.50 * 1
Power supply, 12 V
12151.99
1
Software Cobra3 - Temperature
14503.61 * 1
Semiconductor temperature sensor, –20...110°C
12120.00 * 2
Precision thermometer, –10...+100°C
47040.00
1
Rubber bands
03920.00
1
Rubber gloves
46359.00
1
Adhesive tape Ventilator PC, Windows® 95 or higher
Regulation of human body temperature with Cobra3 BASIC-UNIT
P4060311
Typical result for experiment 2
Changes in the equipment required for use of the CHEM-UNIT: (Instead of * above-mentioned) Cobra3 CHEM-UNIT
12153.00
Data cable, plug/socket, 9 pole
14602.00
1 1
Software Cobra3 CHEM-UNIT
14520.61
1
Immersion probe NiCr-Ni
13615.03
2
Regulation of human body temperature with Cobra3 CHEM-UNIT
P4060340
Experimental set-up using the CHEM-UNIT (P4060340)
36 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
7
Visual Sense Contents LEB 07.01
Optomotor response in insects
LEB 07.02
Determination of the human visual field
LEB 07.03
Time resolving capability of the human eye
LEB 07.04
Test of human reaction capacity
LEB 07.05
Human electrooculography (EOG)
LEB 07.06
Additive and subtractive colour mixing and colour maskings
Visual Sense
LEB 07
07.01 Optomotor response in insects What you can learn about Optomotor response Time resolving capability Compound eye Strobe drum Pattern frequency Left- and righthand movements of flies
Principle and tasks: Behavioural physiological measurement of the time resolving capacity of the compound eye of flies (without electrophysiological leads). To determine the ratio of right and left turns made by a fly attached to the centre of a strobe drum. The movements (optomotor response) indicate whether or not the fly can resolve the strobe pattern with the drum set at a constant speed.
What you need: Strobe drum
65976.00
1
Support base, variable
02001.00
1
Right-angle clamp (bosshead)
02043.00
2
Support rod, stainless steel, l = 500 mm
02032.00
2
Support rod, stainless steel, l = 250 mm
02031.00
1
Universal clamp
37715.00
1
Motor with disk holder, 12 V
11614.00
1
Power supply, 0...12 V, 2 A
13505.93
1
Snap-cap vials, 60 ml
33623.03
1 1
Glass tube, straight, l = 80 mm
36701.65
Rubber tubing, di = 6 mm
39282.00
1
Compressed gas, CO2, 12 I
41772.06
1
Fine control valve
33499.00
1
Iron wires, 140 mm
45286.00
1
Rubber stopper, d = 27/21 mm, 1 hole
39257.01
1
Scissors, straight, pointed, l = 110 mm
64623.00
1
Connecting cord, 32 A, l = 750 mm, red
07362.01
1 1
Connecting cord, 32 A, l = 750 mm, blue
07362.04
Acetone, chem. pure, 250 ml
30004.25
1
Caterpillar breeding box
64564.00
1
Fastening the fly in place
Flies Sugar cubes 10-second glue Typing or flimsy paper
Optomotor response in insects P4070100
38 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
Visual Sense
LEB 07
Determination of the human visual field 07.02 What you can learn about Perimeter Visual field (for white, blue, red, green) Field of view Blind spot Scotoma Rods and cones
Principle and tasks: Determination of the visual field of the right and left eye for white, blue, red and green. Detection of any visual field deficiency (scotoma). Location of the blind spot (site of optic nerve emergence). The extent of the visual fields of both eyes and the position of the blind spot are determined with the aid of a perimeter.
What you need: Perimeter
65984.00
1
Support rod, l = 500 mm, stainless
02032.00
1
Bench clamp, “PASS”
02010.00
1
Right-angle clamp
02043.00
1
Protractor scale with pointer
08218.00
1
Stand tube
02060.00
2
Support base, variable
02001.00
1
Table top on rod
08060.00
1
Determination of the human visual field P4070200
Visual field of a right eye
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 39
Visual Sense
LEB 07
07.03 Time resolving capability of the human eye What you can learn about Perimeter Time-related resolving power Flicker fusion frequency Light/dark adapted eye
Principle and tasks: To determine the flashing frequency of an LED at which the impression of a continuous light just occurs. To change the direction of incidence of the light using a perimeter. To determine the flicker fusion threshold of the left and right eye in relation to the direction of incidence of light stimulus and the state of adaptation of the eyes.
What you need: Sine wave generator
65960.93
1
Stimulant light source
65985.00
1
Perimeter
65984.00
1
Right-angle clamp
02043.00
1
Table top on rod
08060.00
1
Bench clamp, “PASS”
02010.00
1
Support base, variable
02001.00
1
Support rod, l = 500 mm, stainless
02032.00
1
Stand tube
02060.00
1
Time resolving capability of the human eye P4070300
Flicker fusion frequency curve
40 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Visual Sense
LEB 07
Test of human reaction capacity 07.04 What you can learn about Reaction capacity Strobe drum Control loop Feedback reaction Dead time Follow-through time Threshold frequency
Principle and tasks: Measurement of dead time in a reaction test. Analysis of the transient response. Determination of threshold frequency for stimuli. Study of the effects of noise and alcohol on reaction capacity. The test subject should follow a rectangular curve on a slowly rotating drum, using a felt-tip pen inserted into a slit.
What you need: Strobe drum
65976.00
1
Reaction test sheets
65976.02
1
Motor with disk holder, 12 V
11614.00
1
Power supply, 0...12 V, 2 A
13505.93
1
Connecting cord, 32 A, l = 750 mm, red
07362.01
1
Connecting cord, 32 A, l = 750 mm, blue
07362.04
1
Support base, variable
02001.00
1
Plate holder
02062.00
1
Right-angle clamp (bosshead)
02043.00
2
Support rod, l = 250 mm, stainless
02031.00
1
Support rod, l = 500 mm, stainless
02032.00
2
Felt-tip pen Reaction curve
Adhesive tape (transparent)
Test of human reaction capacity P4070400
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
Laboratory Experiments, Biology 41
Visual Sense
LEB 07
07.05 Human electrooculography (EOG) What you can learn about Electrophysiology Electrical field measurement Eye movements Dipole Sacchades Fixation period Practised reader versus schoolchild Rapid reading techniques
Principle and tasks: To record the changes in the electrical field induced by eye movements, using electrodes stuck to the skin near the eyes. To measure an electrooculogram (EOG) with a practised reader, a less practised (six year old) schoolchild and, if possible, a test person who practises a rapid reading technique. To evaluate the rapid horizontal eye movements (sacchades) and the fixation periods.
Attaching the electrodes
What you need: Cobra3 BASIC-UNIT, USB
12150.50
Power supply, 12 V
12151.99
1 1
Software Cobra3 Universal Recorder
14504.61
1
Bio-amplifier
65961.93
1
EMG electrodes
65981.02
1
Electrode collecting cable
65981.03
1
Electrode cream, tube
65981.05
1
Connecting cord, 32 A, l = 250 mm, red
07360.01
1
Connecting cord, 32 A, l = 250 mm, blue
07360.04
1
Roll of adhesive tape (e.g. Elastoplast) PC, Windows® 95 or higher
Human electrooculography (EOG)
Typical result
P4070511
42 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Visual Sense
LEB 07
Additive and subtractive colour mixing and colour maskings 07.06 What you can learn about Additive colour mixing Subtractive colour mixing Complementary colour Colour saturation White masking Black masking
Principle and tasks: Production of mixed colours and white light by superimposed projection (additive mixing) of red, blue and green light. Brightening of a colour spot (white masking) or its surroundings (black masking) by means of white light.
What you need: Three colour lamp
13760.00
1
Set of colour filters
13760.01
1
Brightness adjuster
13760.93
1
Negative hole diaphragm, d = 20 mm
17556.00
1
Circular diaphragm, with hole, d = 20 mm
09816.01
1
Screen, metal, 300 x 300 mm
08062.00
1
Support base, variable
02001.00
1
Barrel base ”PASS”
02006.55
1
Additive and subtractive colour mixing and colour maskings
P4070600
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 43
44
8
Behaviour Contents LEB 08.01
Wall contact searching in mice
LEB 08.02
Learning and marking behaviour of mice
LEB 08.03
Learning performance of humans
Behaviour
LEB 08
08.01 Wall contact searching in mice What you can learn about Behaviour of mice Search for wall contact Distribution of staying places Random distribution Percentage deviation
Principle and tasks: To record the amount of time spent by mice on a platform with and without walls divided into 49 squares numbered in sequence. To compare the recorded distribution of staying places with the calculated random distribution. To calculate the percentage deviation.
What you need: Platform for behaviour observation
65991.00
1
White mice
Wall contact searching in mice P4080100
46 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
Behaviour
LEB 08
Learning and marking behaviour of mice 08.02 What you can learn about Y-Labyrinth High labyrinth Operative conditioning Learning Curve Marking behaviour
Principle and tasks: To test the learning process in a simple Y-Iabyrinth (train to the left and right) and on a complicated high labyrinth. To measure the time taken to cross the maze and the number of errors in relation to previous experience and scent marking. To draw learning curves for different labyrinths and animals.
What you need: High labyrinth
65992.00
1
Y-Labyrinth
65993.00
1
White mice Food
Learning and marking behaviour of mice P4080200
High labyrinth
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
Laboratory Experiments, Biology 47
Behaviour
LEB 08
08.03 Learning performance of humans What you can learn about Finger labyrinth Learning behaviour Learning curve Short term memory Medium term memory
Principle and tasks: The blindfolded test subject has to find the way to the finish with a felt-tip pen in the slits of a finger labyrinth. Success and error are checked by placing a sheet of paper underneath. The learning curves for two different test objectives are drawn: to cross the maze with the least number of errors and as quickly as possible.
What you need: Finger labyrinth
65990.00
1
Stop watch, interruption type
03076.01
1
Blindfold or opaque glasses Selection of coloured felt-tip pens
Learning performance of humans P4080300
48 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
9
Respiration Contents LEB 09.01
Volumetric measurement of breathing in small animals
LEB 09.02
Measurement of the human respiratory rate
Respiration
LEB 09
09.01 Volumetric measurement of breathing in small animals What you can learn about Oxygen consumption Volumetric measurement Respirometer Carbon dioxide consumption Compensation vessel Q10 value
Principle and tasks: Measure the oxygen consumption of insects in relation to ambient temperature and body weight. Volumetric measurement of the oxygen consumption of an animal enclosed in a temperature-controlled spirometer. Absorption of the exhaled carbon dioxide using concentrated potassium hydroxide solution.
What you need: Respirometer, compact model
65998.00
1
Immersion thermostat TC10
08492.93
1
Bath for thermostat, 6 l,
08487.02
1
Support base, variable
02001.00
1
Support rod, l = 250 mm, stainless
02031.00
2
Right-angle clamp (bosshead)
02043.00
1
Balance SAS 51, 200 g / 0.01 g, RS232
45990.93
1
Volumetric pipette, 10 ml
36578.00
1
Pipettor
36592.00
1
Tweezers, straight, pointed, 120 mm
64607.00
1
Glass beads, d = 6 mm, 850 pieces
36756.25
1
Silicone High Vacuum Grease, 100 g
31863.10
1
Potassium hydroxide sol., 30%, 500 ml
48209.50
1
Mealworms and meal beetles Ink solution
Volumetric measurement of breathing in small animals
P4090100
Respirometer
50 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
Respiration
LEB 09
Measurement of the human respiratory rate 09.02 What you can learn about Respiratory frequency Chest pressure measurement Breathing in resting position In slight and strong exertion Eupnea Diaphragmatic and thoracic respiration
Principle and tasks: The number of inhalations per unit time is dependent on many factors, such as the capacity of the lungs, health condition and activity. The respiratory frequencies before and after bodily exertion are to be measured and compared.
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 - Pressure
14510.61
1
Measuring module, pressure
12103.00
1
Blood pressure measurement set
64234.00
1
Y piece
47518.01
1
Additionally required: Kidney protective belt (motor cycle accessory) PC, Windows® 95 or higher
Result (at rest)
Measurement of the human respiratory rate with Cobra3 BASIC-UNIT
P4090211
Result (during slight exertion)
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 51
52
10
Ecology and environment Contents
LEB 10.01
Comparison of the heat capacities of water and land
LEB 10.02
The Bergmann rule: Heat loss in dependence on body surface area and volume
LEB 10.03
The insulating effect of body coverage
LEB 10.04
The daily course of luminosity
LEB 10.05
The conductivity of various water samples
LEB 10.06
The pH of various water samples
LEB 10.07
The origin of acid rain
LEB 10.08
The twenty-four hour rhythm of an aquatic plant
Ecology and environment
LEB 10
10.01 Comparison of the heat capacities of water and land What you can learn about Heat capacity Heat radiation Origin of climatic fluctuations Generation of onshore and offshore winds
Principle and tasks: To prepare comparison curves showing the different heat capacities of water and land. To interpret data on climate and to explain how onshore and offshore winds originate.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB
12150.50 * 1
Power supply, 12 V
12151.99
Software Cobra3 - Temperature
14503.61 * 1
1
Semiconductor temperature sensor, -20...110°C
12120.00 * 2
Support base, variable
02001.00
1
Bosshead
02043.00
1
Support rod, l = 500 mm, stainless steel
02032.00
1
Filament lamp, 220 V/120 W, with reflector
06759.93
1
Lamp socket E 27, with reflector
06751.01
1
Plastic dish, 230 x 150 mm
47490.04
2
Precision thermometer, –10...+100°C
47040.00
1
Sea sand, purified
30220.67
1
Typical result
Polystyrene foam PC, Windows® 95 or higher
Comparison of the heat capacities of water and land with Cobra3 BASIC-UNIT P4100111 Changes in the equipment required for use of the CHEM-UNIT: (Instead of * above-mentioned) Cobra3 CHEM-UNIT
12153.00
Data cable, plug/socket, 9 pole
14602.00
1 1
Software Cobra3 CHEM-UNIT
14520.61
1
Immersion probe NiCr-Ni
13615.03
2
Comparison of the heat capacities of water and land with Cobra3 CHEM-UNIT P4100140
Experimental set-up using the CHEM-UNIT (P4100140)
54 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Ecology and environment
LEB 10
The Bergmann rule: Heat loss in dependence on body surface area and volume 10.02 What you can learn about Bergman rule, heat loss Larger versus smaller animals Colder versus warmer regions of the world
Principle and tasks: To prepare a graph which demonstrates the different heat losses with different volumes and body surface areas. To interpret data on the settling of warm and cold regions by larger or smaller animals.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB
12150.50 * 1
Power supply, 12 V
12151.99
Software Cobra3 - Temperature
14503.61 * 1
1
Semiconductor temperature sensor -20...110°C
12120.00 * 2
Support base, variable
02001.00
1
Universal clamp
37715.00
2
Bosshead
02043.00
2
Support rod, l = 500 mm, stainless steel
02032.00
1
Flask, round-bottom, 100 ml, IGJ 19/26
35841.01
1
Long-neck round-bottom flask, 500 ml, PN 29
36348.00
1
Rubber stopper, d = 22/17 mm, 1 hole
39255.01
1
Rubber stopper, d = 32/26 mm, 1 hole
39258.01
1
Precision thermometer, –10...+100°C
47040.00
1
Typical result
PC, Windows® 95 or higher
The Bergmann rule: Heat loss in dependence on body surface area and volume with Cobra3 BASIC-UNIT P4100211 Changes in the equipment required for use of the CHEM-UNIT: (Instead of * above-mentioned) Cobra3 CHEM-UNIT
12153.00
1
Data cable, plug/socket, 9 pole
14602.00
1
Software Cobra3 CHEM-UNIT
14520.61
1
Immersion probe NiCr-Ni
13615.03
2
The Bergmann rule: Heat loss in dependence on body surface area and volume with Cobra3 CHEM-UNIT P4100240 Experimental set-up using the CHEM-UNIT (P4100240)
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 55
Ecology and environment
LEB 10
10.03 The insulating effect of body coverage What you can learn about Insulating effect Body coverage Coat of animals Fur Wool Feathers
Principle and tasks: To prepare curves showing the insulating effect of various body coverage’s. To discuss the reasons of different isolating characteristics.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB
12150.50 * 1
Power supply, 12 V
12151.99
Software Cobra3 - Temperature
14503.61 * 1
1
Semiconductor temperature sensor -20...110°C
12120.00 * 2
Snap-cap vials, 45 ml, pack of 10
33622.03
1
Precision thermometer, –10...+100°C
47040.00
1
Leatherwool (fur), wool, feathers etc. Polystyrene foam PC, Windows® 95 or higher
The insulating effect of body coverage with Cobra3 BASIC-UNIT
P4100311
Typical result
Changes in the equipment required for use of the CHEM-UNIT: (Instead of * above-mentioned) Cobra3 CHEM-UNIT
12153.00
1
Data cable, plug/socket, 9 pole
14602.00
1
Software Cobra3 CHEM-UNIT
14520.61
1
Immersion probe NiCr-Ni
13615.03
2
The insulating effect of body coverage with Cobra3 CHEM-UNIT
P4100340
Experimental set-up using the CHEM-UNIT (P4100340)
56 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Ecology and environment
LEB 10
The daily course of luminosity 10.04 What you can learn about Long-term measurement Course of luminosity Seasonal influences Geographical location Cloudiness Fog
Principle and tasks: In a 24-hour long-term measurement the course of luminosity is recorded in the immediate vicinity of a window. The dependence on the season, the geographical location and the current degree of cloudiness, fog etc. has to be discussed.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB
12150.50 * 1
Power supply, 12 V
12151.99
Software Cobra3 Universal Recorder
14504.61 * 1
1
Photodiode in plug-in box
39119.01
Support for two electrodes
45284.01
1 1
Support base, variable
02001.00
1
Connecting cord, 32 A, l = 500 mm, red
07361.01
1
Connecting cord, 32 A, l = 500 mm, blue
07361.04
1
PC, Windows® 95 or higher
The daily course of luminosity with Cobra3 BASIC-UNIT
P4100411
Typical measuring result
Changes in the equipment required for use of the CHEM-UNIT: (Instead of * above-mentioned) Cobra3 CHEM-UNIT
12153.00
1
Data cable, plug/socket, 9 pole
14602.00
1
Software Cobra3 CHEM-UNIT
14520.61
1
The daily course of luminosity with Cobra3 CHEM-UNIT
P4100440
Experimental set-up using the CHEM-UNIT (P4100440)
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 57
Ecology and environment
LEB 10
10.05 The conductivity of various water samples What you can learn about Conductivity of water samples Rainwater Drinking water Aquarium water River water Mineral water Electrolytes Waste water Eluvation Contamination by inorganic salts Horizontal/vertical zones
Principle and tasks: To determine the conductivity of various water samples. To discuss the reasons for the differences in conductivity.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT
12153.00 * 1
Power supply, 12 V
12151.99
1
Data cable, plug/socket, 9 pole
14602.00
1
Software Cobra3 CHEM-UNIT
14520.61 * 1
Conductivity-temperature electrode
13701.01 * 1
Wash bottle, 500 ml
33931.00
1
Beaker, DURAN, tall form, 50 ml
36001.00
1
Beaker, DURAN, tall form, 150 ml
36003.00
7
Support base, variable
02001.00
1
Support rod, l = 500 mm, stainless steel
02032.00
1
Right angle clamp (bosshead)
02043.00
1
Universal clamp
37715.00
1
Calibration solution, 1413 mS/cm, (25°C)
47070.02
1
Distilled water, 5 l
31246.81
1
Display of the measured conductivity values
Water samples: Rainwater, drinking water, aquarium water, river water, mineral water (2 different sorts) PC, Windows® 95 or higher
The conductivity of various water samples with Cobra3 CHEM-UNIT
P4100540
Changes in the materials required for use of the BASIC-UNIT: (Instead of * above-mentioned) Cobra3 BASIC-UNIT, USB
12150.50
Software Cobra3 conductivity
14508.61
1
Measuring module conductivity
12108.00
1
Conductivity probe, glass shaft, l = 12 cm
18151.02
1
Temperature sensor, semiconductor type
12120.00
1
The conductivity of various water samples with Cobra3 BASIC-UNIT 58 Laboratory Experiments, Biology
1
Experimental set-up using the BASIC-UNIT (P4100511)
P4100511 PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Ecology and environment
LEB 10
The pH of various water samples 10.06 What you can learn about pH of water samples Demineralised water Aquarium water Rainwater Lake water River water Drinking water Mineral water Seasonal and daily fluctuations Buffering effect Poisoning effect for animals
Principle and tasks: To determine the pH-values of various water samples. To discuss the reasons for the differences in pHvalue.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT Power supply, 12 V Data cable, plug/socket, 9 pole
12153.00 * 1 12151.99 1 14602.00 1
Software Cobra3 CHEM-UNIT pH electrode, gel-filled, BNC Immersion probe NiCr-Ni, Teflon Wash bottle, 500 ml Beaker, DURAN, tall form, 50 ml Beaker, DURAN, tall form, 150 ml Support base, variable Support rod, l = 500 mm, stainless steel Right angle clamp (bosshead) Double electrode holder Buffer solution pH 4.01, 460 ml Buffer solution pH 10.01, 1000 ml Distilled water, 5 l Water samples: Aquarium water, rainwater, lake water, river water, drinking water, mineral water PC, Windows® 95 or higher
14520.61 * 46265.15 * 13615.05 * 33931.00 36001.00 36003.00 02001.00 02032.00 02043.00 45284.10 46270.11 46272.12 31246.81
The pH of various water samples with Cobra3 CHEM-UNIT
1 1 1 1 2 7 1 1 1 1 1 1 1
Display of the measured pH values
P4100640
Changes in the materials required for use of the BASIC-UNIT: (Instead of * above-mentioned) Cobra3 BASIC-UNIT, USB Software Cobra3 pH/potential Measuring module pH/potential pH Electrode, plastic, gel-filled Temperature sensor, semiconductor type
12150.50 14509.61 12101.00 18450.00 12120.00
1 1 1 1 1 Experimental set-up using the BASIC-UNIT (P4100611)
The pH of various water samples with Cobra3 BASIC-UNIT
P4100611
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 59
Ecology and environment
LEB 10
10.07 The origin of acid rain What you can learn about Acid rain Anthropogenic air pollution Damage to forests Acidification of soil and water Gaseous and aerosol emissions
Principle and tasks: In this experiment, acid rain is to be artificially prepared by passing gases such as SO2, NO2 and CO2 through water, and the drop in pH resulting from the formation of acid then recorded.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT 12153.00 * Power supply, 12 V 12151.99 Data cable, plug/socket, 9 pole 14602.00 Software Cobra3 CHEM-UNIT 14520.61 * pH electrode, gel-filled, BNC 46265.15 * Support base, variable 02001.00 Support rod, l = 500 mm, stainless steel 02032.00 Right angle clamp (bosshead) 02043.00 Universal clamp 37715.00 Test tube, DURAN, GL 25/12, 20 x 110 mm 37651.15 Graduated pipette, AR-glass, class B, 5 ml 36599.00 Wash bottle, 500 ml 33931.00 Beaker, DURAN, tall form, 50 ml 36001.00 Pasteur pipettes, PE, 1 ml, pack of 500 36615.00 Test tube rack for 12 tubes, wood 37686.10 Test tube, DURAN, with hose connection, PN 19 36330.00 Rubber stopper, d = 22/17 mm, without hole 39255.00 Rubber stopper, d = 22/17 mm, 1 hole 39255.01 Dropping funnel with stopcock, 50 ml, AR-glass 36869.00 Spoon with spatula end, l = 150 mm, steel, wide 33398.00 Buffer solution pH 4.01, 460 ml 46270.11 Buffer solution pH 10.01, 1000 ml 46272.12 Sodium carbonate, 250 g 30154.25 Sodium pyrosulphite, 250 g 30152.25 Sodium nitrite, 250 g 30161.25 Hydrochloric acid standard solution, 1 mol/l, 1000 ml 48454.70 Dropping bottle, plastic, 50 ml 33920.00 Glycerol 99%, 100 ml 30084.10 Distilled water, 5 l 31246.81 Fume cupboard or fume hood PC, Windows® 95 or higher
1 1 1 1 1 1 1 2 2 1 1 1 2 1 1 3 2 1 1 1 1 1 1 1 1 1 1 1 1
Curve of pH against time for SO2
The origin of acid rain with Cobra3 CHEM-UNIT P4100740 Changes in the materials required for use of the BASIC-UNIT: (Instead of * above-mentioned) Cobra3 BASIC-UNIT, USB 12150.50 1 Software Cobra3 pH/potential 14509.61 1 Measuring module pH/potential 12101.00 1 pH Electrode, plastic, gel-filled 18450.00 1
Experimental set-up using the BASIC-UNIT (P4100711)
The origin of acid rain with Cobra3 BASIC-UNIT P4100711 60 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Ecology and environment
LEB 10
The twenty-four hour rhythm of an aquatic plant 10.08 What you can learn about Photosynthesis Daylight reaction Dark reaction Diurnal rhythm Nocturnal rhythm pH measurement Carbon dioxide consumption and production
Principle and tasks: Plants produce oxygen by photosynthesis under the influence of light (during the day). Carbon dioxide is hereby consumed. In the dark (at night), however, they consume oxygen and produce carbon dioxide. This day and night rhythm in an aquatic plant has to be followed over a longer period by pH measurements.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT Power supply, 12 V Data cable, plug/socket, 9 pole Software Cobra3 CHEM-UNIT pH electrode, gel-filled, BNC Filament lamp with reflector, 230 V/120 W Lamp socket E27, with reflector Support base variable Wash bottle, 500 ml Beaker, DURAN, tall form, 50 ml Beaker, DURAN, tall form, 600 ml Beaker, DURAN, tall form, 2000 ml Erlenmeyer flask, DURAN, 500 ml, PN 29 Rubber stopper, d = 32/30 mm, 1 hole, 14 mm Supporting block, 57 mm Aquarium, 400 x 250 x 250 mm Compressed gas in pressure bottle, oxygen Fine control valve for pressure bottles Diffuser Rubber tubing, di = 4 mm Balance SAS 51, 200 g / 0.01 g, RS232 Spoon with spatula end, l = 150 mm, steel, wide Glass rod, d = 7 mm, l = 300 mm Buffer solution pH 4.01, 460 ml Buffer solution pH 10.01, 1000 ml Hydrochloric acid standard solution, 0.1 mol/l, 1000 ml Sodium carbonate, 250 g Distilled water, 5 l Waterweed (Elodea), approx. 4 medium size plants PC, Windows® 95 or higher
The twenty-four hour rhythm of an aquatic plant with Cobra3 CHEM-UNIT
12153.00 12151.99 14602.00 14520.61 46265.15 06759.93 06751.01 02001.00 33931.00 36001.00 36006.00 36010.00 36421.00 39258.20 02073.00 64559.00 41772.05 33499.00 64567.00 39280.00 45990.93 33398.00 40485.05 46270.11 46272.12 48452.70 30154.25 31246.81
1 1 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1
Curve of pH against time for the reaction of waterweed in daylight
Curve of pH against time for the reaction of waterweed in the dark
P4100840
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 61
62
1
11
Plant physiology Contents
LEB 11.01
Photosynthesis (bubble counting method)
LEB 11.02
Photosynthesis (measurement of O2 pressure)
LEB 11.03
Transpiration of leaves
LEB 11.04
Glycolysis (measurement of pressure)
LEB 11.05
Glycolysis (measurement of temperature)
Plant physiology
LEB 11
11.01 Photosynthesis (bubble counting method) What you can learn about Dependence of photosynthesis on light and carbon dioxide content Oxygen bubble counting Lux measurement
Principle and tasks: To measure the dependence of photosynthesis on light by counting the oxygen bubbles given off by an aquatic plant. To investigate the influence of the carbon dioxide content of the water on the rate of photosynthesis.
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Power supply, 12 V
12151.99
1
Software Cobra3 - Lux
14507.61
1
Photodiode in plug-in box
39119.01
1
Support for two electrodes
45284.01
1
Connecting cord, 500 mm, red
07361.01
1
Connecting cord, 500 mm, blue
07361.04
1
Support base, variable
02001.00
1
Lab-lifting apparatus, 160 x 130 mm
02074.00
1
Filament lamp, 220 V/120 W, with reflector
06759.93
1
Lamp socket E 27, with reflector
06751.01
1
Glass beaker, 1000 ml, tall form, DURAN
36008.00
1
Glass beaker, 250 ml, tall form, DURAN
36004.00
1
Rubber tubing, di = 6 mm, 1 m
39282.00
1
Result
Sparkling mineral water Water-weed PC, Windows® 95 or higher
Photosynthesis (bubble counting method) with Cobra3 BASIC-UNIT
64 Laboratory Experiments, Biology
P4110111
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Plant physiology
LEB 11
Photosynthesis (measurement of O2 pressure) 11.02 What you can learn about Photosynthesis Intensity and colour of the light Pressure measurement
Principle and tasks: To compare the increase in oxygen pressure during photosynthesis in white light, green light and in the dark.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB 12150.50 Power supply, 12 V 12151.99 Software Cobra3 - Pressure 14510.61 Measuring module, pressure 12103.00 Support clamp for small case 02043.10 Support base, variable 02001.00 Support rod, l = 500 mm, stainless steel 02032.00 Bosshead 02043.00 Universal clamp 37715.00 Lab-lifting apparatus, 160 x 130 mm 02074.00 Filament lamp, 220 V/120 W, with reflector 06759.93 Lamp socket E 27, with reflector 06751.01 Glass beaker, 1000 ml, tall form, DURAN 36008.00 Rubber stopper, d = 32/26 mm, 1 hole 39258.01 Rubber tubing, di = 6 mm, 1 m 39282.00 Test tube, d = 30 mm, l = 200 mm, PN29 36294.00 Glass tubes, straight, 80 mm, d = 7 mm 36701.65 Glass rod, l = 200 mm, d = 3 mm 40485.01 Balance SAS 51, 200 g/0.01 g, RS232 45990.93 Microspoon, special steel 33393.00 Glycerol, 99%, 100 ml 30084.10 Dropping bottle, plastic, 50 ml 33920.00 Sodium hydrogen carbonate, 250 g 30151.25 Tartrazine (E 102), 25 g 48498.04 Patent blue V (E 131), 25 g 48376.04 Aluminium foil Water-weed PC, Windows® 95 or higher
1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Result with white light
Result in the dark
Photosynthesis (measurement of O2 pressure) with Cobra3 BASIC-UNIT
P4110211
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 65
Plant physiology
LEB 11
11.03 Transpiration of leaves What you can learn about Transpiration Water and nutrients transport Influence of wind Temperature Humidity and type of leaves
Principle and tasks: To prepare diagrams to illustrate the transpiration of plants. To discuss the reasons of the pressure decrease.
What you need: Cobra3 BASIC-UNIT, USB
12150.50
1
Support clamp for small case
02043.10
1
Power supply, 12 V
12151.99
1
Software Cobra3 - Pressure
14510.61
1
Measuring module, pressure
12103.00
1
Support base, variable
02001.00
1
Support rod, l = 500 mm, stainless
02032.00
3
Bosshead
02043.00
1
Universal clamp
37715.00
1
Test tube, 200 x 30 mm, side arm, PN 29
36331.00
1
Rubber stopper, d = 32/26 mm, 1 hole
39258.09
1
Rubber tubing, di = 6 mm, 1 m
39282.00
1
Glass beaker, 1000 ml, short form, DURAN
36017.00
1
Tripod, ring dia 100 mm, h = 180 mm
33299.00
1
Wire gauze, 120 x 120 mm
33287.03
1
Bunsen burner, natural gas
32165.05
1
Safety gas tubing, 1 m
39281.10
1
Tubing clip, d = 8...12 mm
40996.01
2
Rubber bands, 50 off
03920.00
1
Hot/cold air blower, 1700 W
04030.93
1
Result in still air
PC, Windows® 95 or higher Plastic bag Knife Flesh-leaved plant
Transpiration of leaves with Cobra3 BASIC-UNIT P4110311
66 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Plant physiology
LEB 11
Glycolysis (measurement of pressure) 11.04 What you can learn about Glycolysis, yeast fermentation of sugar CO2 pressure measurement Influence of temperature and pH
Principle and tasks: To identify glycolysis by measuring the production of CO2 and plotting graphs. To investigate the influence of temperature and pH on metabolic activity.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB 12150.50 Power supply, 12 V 12151.99 Software Cobra3 - Pressure 14510.61 Measuring module, pressure 12103.00 Support clamp for small case 02043.10 Magnetic stirrer, mini 47334.93 Magnetic stirrer bar, l = 50 mm 46299.03 Glass beaker, 1000 ml, short form, DURAN 36017.00 Glass beaker, 250 ml, tall form, DURAN 36004.00 Graduated pipette, 10 ml 36600.00 Erlenmeyer flask, narrow neck, PN 29 36424.00 Rubber stopper, d = 32/26 mm, 1 hole 39258.01 Glass tubes, straight, 80 mm, d = 7 mm 36701.65 Rubber tubing, di = 6 mm, 1 m 39282.00 Bunsen burner, natural gas 32165.05 Safety gas tubing, 1 m 39281.10 Tubing clip, d = 8...12 mm 40996.01 Tripod, ring dia 100 mm, h = 180 mm 33299.00 Wire gauze, 120 x 120 mm 33287.03 Glass rod, l = 200 mm, d = 3 mm 40485.01 Support base, variable 02001.00 Support rod, l = 500 mm, stainless steel 02032.00 Bosshead 02043.00 Universal clamp 37715.00 Balance SAS 51, 200 g/0.01 g, RS232 45990.93 Precision thermometer, –10...+100°C 47040.00 Buffer solution, pH 10.01, 1000 ml 46272.12 Buffer solution, pH 4.01, 460 ml 46270.11 Glycerol, 99%, 100 ml 30084.10 Dropping bottle, plastic, 50 ml 33920.00 Grape juice Fresh baker’s yeast Ice cubes PC, Windows® 95 or higher
1 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1
Result at room temperature
Result in iced water
Glycolysis (measurement of pressure) with Cobra3 BASIC-UNIT
P4110411
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 67
Plant physiology
LEB 11
11.05 Glycolysis (measurement of temperature) What you can learn about Glycolysis Yeast fermentation of sugar Temperature measurement Respiration energy
Principle and tasks: To depict the increase in temperature, which results, when yeast cells ferment sugar. To compare the temperature curves of the solutions with and without yeast.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB
12150.50 * 1
Power supply, 12 V
12151.99
Software Cobra3 - Temperature
14503.61 * 1
1
Semiconductor temperature sensor -20...110°C
12120.00 * 2
Precision thermometer, –10...+100°C
47040.00
1
Balance, SAS 51, 200 g/0.01 g, RS232
45990.93
1
Thermos flask, 0.5 l
64841.00
2
Rubber stopper, d = 41/34 mm, 2 holes
39261.02
2
Glass beaker, 1000 ml, short form, DURAN
36017.00
1
Fresh baker’s yeast Refined sugar, crystalline Typical result
PC, Windows® 95 or higher
Glycolysis (measurement of temperature) with Cobra3 BASIC-UNIT
P4110511
Changes in the equipment required for use of the CHEM-UNIT: (Instead of * above-mentioned) Cobra3 CHEM-UNIT 12153.00 1 Data cable, plug/socket, 9 pole
14602.00
1
Software Cobra3 CHEM-UNIT
14520.61
1
Immersion probe NiCr-Ni
13615.03
2
Glycolysis (measurement of temperature) with Cobra3 CHEM-UNIT
P4110540
Experimental set-up using the CHEM-UNIT (P4110540)
68 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
12
Biochemistry Contents
LEB 12.01
Determination of the isoelectric point of an amino acid (glycine)
LEB 12.02
The ionic permeability of the cell membrane
LEB 12.03
Determination of the Michaelis constant
LEB 12.04
Substrate inhibition of enzymes
LEB 12.05
Enzyme inhibition (poisoning of enzymes)
LEB 12.06
The enzymatic activity of catalase
Biochemistry
LEB 12
12.01 Determination of the isoelectric point of an amino acid (glycine) What you can learn about Isoelectric point Acidic anions Basic cations Zwitterions Equivalence (inflection) points pKs value Titration Motor piston burette
Principle and tasks: Amino acid molecules carry both acid and amino groups. They can therefore form both acidic anions and basic cations. The pH at which these two types of ions are both present in the same concentration is called the isoelectric point. This point is to be determined by recording the titration curve for the amino acid glycine.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT 12153.00 * Power supply, 12 V 12151.99 Data cable, plug/socket, 9 pole 14602.00 Software Cobra3 CHEM-UNIT 14520.61 * pH electrode, gel-filled, BNC 46265.15 * Immersion probe NiCr-Ni, Teflon 13615.05 * Cable, CHEM-UNIT / motor piston burette 36501.01 * Motor piston burette, universal type, 50 ml 36499.93 Magnetic stirrer mini, plastic (ABS) 47334.93 Magnetic stirring rod, cylindrical, l = 30 mm 46299.02 Volumetric pipette, 50 ml 36581.00 Rubber stopper, 14/18, 1 hole, d = 7 mm 39254.01 Safety pipettor Flip 36592.00 Wash bottle, 500 ml 33931.00 Beaker, DURAN, tall form, 250 ml 36004.00 Beaker, DURAN, tall form, 50 ml 36001.00 Volumetric flask, 500 ml 36551.00 Funnel, glass, d = 55 mm 34457.00 Spoon with spatula end, l = 150 mm, steel, wide 33398.00 Balance SAS 51, 200 g / 0.01 g, RS232 45990.93 Buffer solution pH 4.01, 460 ml 46270.11 Buffer solution pH 10.01, 1000 ml 46272.12 Sodium hydroxide standard solution, 1 mol/l, 1 l 48329.70 Hydrochloric acid standard solution, 1 mol/l, 1 l 48454.70 Glycine, 100 g 31341.10 Distilled water, 5 l 31246.81 PC, Windows® 95 or higher
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1
Titration curve for hydrochloric acid glycine solution against 1 mol/l NaOH
Determination of the isoelectric point of an amino acid (glycine) with Cobra3 CHEM-UNIT P4120140 Changes in the equipment required for use of the BASIC-UNIT: (Instead of * above-mentioned) Cobra3 BASIC-UNIT, USB 12150.50 1 Software Cobra3 pH/potential 14509.61 1 Measuring module pH/potential 12101.00 1 pH Electrode, plastic, gel-filled 18450.00 1 Temperature sensor, semiconductor type 12120.00 1 Cable, BASIC-UNIT / motor piston burette 36501.03 1
Experimental set-up using the BASIC-UNIT (P4120111)
Determination of the isoelectric point of an amino acid (glycine) with Cobra3 BASIC-UNIT P4120111 70 Laboratory Experiments, Biology
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Biochemistry
LEB 12
The ionic permeability of the cell membrane 12.02 What you can learn about Ionic permeability Artificial cell membrane Dialysis tube H+ ions OH- ions Osmotic processes
Principle and tasks: The cell membrane controls the transport of nutrients and water into the cell, and of waste products and water out of the cell. This can take place passively, e.g. on the basis of osmotic processes (differences in concentration), as well as actively. In this experiment, the selective permeability of an artificial cell membrane (dialysis tube) for H+ and OH- ions is to be examined.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT Power supply, 12 V Data cable, plug/socket, 9 pole Software Cobra3 CHEM-UNIT pH electrode, gel-filled, BNC Magnetic stirrer mini, plastic (ABS) Magnetic stirring rod, cylindrical, l = 30 mm Support rod, l = 500 mm, stainless steel Support base, variable Right angle clamp (bosshead) Universal clamp Cylinder, Boro 3, glass base, 25 ml Funnel, glass, d = 55 mm Wash bottle, 500 ml Beaker, DURAN, tall form, 250 ml Beaker, DURAN, tall form, 50 ml Dialysis tubing, d = 24 mm, 1 m Dialysis clips, pack of 2 Disposable gloves, medium, Latex, pack of 100 Buffer solution pH 4.01, 460 ml Buffer solution pH 10.01, 1000 ml Hydrochloric acid standard solution, 1 mol/l, 1000 ml Sodium hydroxide standard solution, 1 mol/l, 1000 ml Distilled water, 5 l PC, Windows® 95 or higher
The ionic permeability of the cell membrane with Cobra3 CHEM-UNIT
12153.00 * 12151.99 14602.00 14520.61 * 46265.15 * 47334.93 46299.02 02032.00 02001.00 02043.00 37715.00 36627.00 34457.00 33931.00 36004.00 36001.00 64208.00 64209.00 46359.00 46270.11 46272.12 48454.70 48329.70 31246.81
1 1 1 1 1 1 1 1 1 2 2 1 1 1 2 2 1 2 1 1 1 1 1 1
pH curve for the emergence of H+ ions
P4120240
Changes in the equipment required for use of the BASIC-UNIT: (Instead of * above-mentioned) Cobra3 BASIC-UNIT, USB Software Cobra3 pH/potential Measuring module pH/potential pH Electrode, plastic, gel-filled
The ionic permeability of the cell membrane with Cobra3 BASIC-UNIT
12150.50 14509.61 12101.00 18450.00
1 1 1 1
pH curve for the emergence of OH- ions
P4120211
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 71
Biochemistry
LEB 12
12.03 Determination of the Michaelis constant What you can learn about Michaelis constant Enzymatic hydrolysis of urea Conductivity measurement Bodenstein principle Enzyme-substrate complex Lineweaver-Burk plot
Principle and tasks: The enzymatic hydrolysis of urea in aqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements can be used to determine the rate of hydrolysis of urea by the enzyme urease at various substrate concentrations. The Michaelis constant can then be calculated from these values.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT Power supply, 12 V Data cable, plug/socket, 9 pole Software Cobra3 CHEM-UNIT Conductivity-temperature electrode Magnetic stirrer mini, plastic (ABS) Magnetic stirring rod, cylindrical, l = 30 mm Magnetic bar separator made of PTFE Balance SAS 51, 200 g / 0.01 g, RS232 Support rod, l = 500 mm, stainless steel Support base, variable Right angle clamp (bosshead) Universal clamp Beaker, DURAN, tall form, 100 ml Erlenmeyer flask, Duran, 100 ml, PN 19 Rubber stopper, d = 22/17, without hole Volumetric pipette, 20 ml Safety pipettor Flip Microliter syringe, 100 ml Pasteur pipettes, l = 145 mm Rubber caps Spoon with spatula end, l = 150 mm, steel, micro Wash bottle, 500 ml Urea, 250 g Urease solution in 50% glycerine, 1000 U/ml, 10 ml Distilled water, 5 l Paper tissues PC, Windows® 95 or higher
Determination of the Michaelis constant with Cobra3 CHEM-UNIT
12153.00 * 12151.99 14602.00 14520.61 * 13701.01 * 47334.93 46299.02 35680.03 45990.93 02032.00 02001.00 02043.00 37715.00 36002.00 36418.00 39255.00 36579.00 36592.00 02606.00 36590.00 39275.03 33393.00 33931.00 30086.25 31924.03 31246.81
1 1 1 1 1 1 1 1 1 1 1 2 1 6 6 6 1 1 1 1 1 1 1 1 1 1
The rate of enzymolysis in dependence on the substrate concentration
P4120340
Changes in the equipment required for use of the BASIC-UNIT: (Instead of * above-mentioned) Cobra3 BASIC-UNIT, USB Software Cobra3 conductivity Measuring module conductivity Conductivity probe, glass shaft, l = 12 cm Temperature sensor, semiconductor type
Determination of the Michaelis constant with Cobra3 BASIC-UNIT 72 Laboratory Experiments, Biology
12150.50 14508.61 12108.00 18151.02 12120.00
1 1 1 1 1
Lineweaver-Burk plot
P4120311 PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Biochemistry
LEB 12
Substrate inhibition of enzymes 12.04 What you can learn about Substrate inhibition Enzymolysis of urea Conductivity-time plot Reaction velocity of enzymatic hydrolysis
Principle and tasks: The enzymatic hydrolysis of urea in aqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements enable the rate of hydrolysis of urea by the enzyme urease to be determined at various substrate concentrations. Inhibition of the enzyme by the substrate occurs at excessive substrate concentrations.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT 12153.00 * Power supply, 12 V 12151.99 Data cable, plug/socket, 9 pole 14602.00 Software Cobra3 CHEM-UNIT 14520.61 * Conductivity-temperature electrode 13701.01 * Magnetic stirrer mini, plastic (ABS) 47334.93 Magnetic stirring rod, cylindrical, l = 30 mm 46299.02 Magnetic bar separator made of PTFE 35680.03 Balance SAS 51, 200 g / 0.01 g, RS232 45990.93 Support rod, l = 500 mm, stainless steel 02032.00 Support base, variable 02001.00 Right angle clamp (bosshead) 02043.00 Universal clamp 37715.00 Beaker, DURAN, tall form, 100 ml 36002.00 Beaker, low form, 250 ml, plastic 36013.01 Erlenmeyer flask, Duran, 100 ml, PN 19 36418.00 Rubber stopper, d = 22/17, without hole 39255.00 Volumetric pipette, 20 ml 36579.00 Safety pipettor Flip 36592.00 Microliter syringe, 100 ml 02606.00 Pasteur pipettes, l = 145 mm 36590.00 Rubber caps 39275.03 Spoon with spatula end, l = 150 mm, steel, micro 33393.00 Wash bottle, 500 ml 33931.00 Urea, 250 g 30086.25 Urease solution in 50% glycerine, 1000 U/ml, 10 ml 31924.03 Distilled water, 5 l 31246.81 Paper tissues PC, Windows® 95 or higher
Substrate inhibition of enzymes with Cobra3 CHEM-UNIT
1 1 1 1 1 1 1 1 1 1 1 2 1 8 1 7 7 1 1 1 1 1 1 1 1 1 1
Conductivity-time plot for the hydrolysis of urea with urease
P4120440
Changes in the equipment required for use of the BASIC-UNIT: (Instead of * above-mentioned) Cobra3 BASIC-UNIT, USB Software Cobra3 conductivity Measuring module conductivity Conductivity probe, glass shaft, l = 12 cm Temperature sensor, semiconductor type
Substrate inhibition of enzymes with Cobra3 BASIC-UNIT
12150.50 14508.61 12108.00 18151.02 12120.00
1 1 1 1 1
The velocity of enzymolysis in dependence on the concentration
P4120411
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 73
Biochemistry
LEB 12
12.05 Enzyme inhibition (poisoning of enzymes) What you can learn about Poisoning of enzymes Enzymatic hydrolysis of urea Silver nitrate inhibition of urease Conductivity measurement
Principle and tasks: The enzymatic hydrolysis of urea in aqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurements enable the rate of hydrolysis of urea by the enzyme urease to be determined. The addition of an appropriate inhibitor poisons the enzyme, so that it no longer converts substrate.
What you need: Equipment (for the experimental procedure using the CHEM-UNIT) Cobra3 CHEM-UNIT 12153.00 * Power supply, 12 V 12151.99 Data cable, plug/socket, 9 pole 14602.00 Software Cobra3 CHEM-UNIT 14520.61 * Conductivity-temperature electrode 13701.01 * Magnetic stirrer mini, plastic (ABS) 47334.93 Magnetic stirring rod, cylindrical, l = 30 mm 46299.02 Magnetic bar separator made of PTFE 35680.03 Balance SAS 51, 200 g / 0.01 g, RS232 45990.93 Support rod, l = 500 mm, stainless steel 02032.00 Support base, variable 02001.00 Right angle clamp (bosshead) 02043.00 Universal clamp 37715.00 Beaker, DURAN, tall form, 100 ml 36002.00 Beaker, low form, 250 ml, plastic 36013.01 Volumetric pipette, 1 ml 36575.00 Volumetric pipette, 20 ml 36579.00 Safety pipettor Flip 36592.00 Microliter syringe, 100 ml 02606.00 Pasteur pipettes, l = 145 mm 36590.00 Rubber caps 39275.03 Spoon with spatula end, l = 150 mm, steel, micro 33393.00 Wash bottle, 500 ml 33931.00 Urea, 250 g 30086.25 Urease solution in 50% glycerine, 1000 U/ml, 10 ml 31924.03 Silver nitrate, 15 g 30222.00 Distilled water, 5 l 31246.81 PC, Windows® 95 or higher
Enzyme inhibition (poisoning of enzymes) with Cobra3 CHEM-UNIT
1 1 1 1 1 1 1 1 1 1 1 1 1 8 1 1 1 1 1 1 1 1 1 1 1 1 1
P4120540
Changes in the equipment required for use of the BASIC-UNIT: (Instead of * above-mentioned) Cobra3 BASIC-UNIT, USB 12150.50 1 Software Cobra3 conductivity 14508.61 1 Measuring module conductivity 12108.00 1 Conductivity probe, glass shaft, l = 12 cm 18151.02 1 Temperature sensor, semiconductor type 12120.00 1
Enzyme inhibition (poisoning of enzymes) with Cobra3 BASIC-UNIT 74 Laboratory Experiments, Biology
Conductivity-time plot for the hydrolysis of urea with urease before and after poisoning
Experimental set-up using the BASIC-UNIT (P4120540)
P4120511 PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Biochemistry
LEB 12
The enzymatic activity of catalase 12.06 What you can learn about Enzyme catalase Decomposition of H2O2 Poisonous by-product of cell respiration Influence of temperature and pH
Principle and tasks: To examine the enzymatic decomposition of hydrogen peroxide, a cell respiratory poison, in the liver. To investigate the influence of temperature and pH on the metabolic activity.
What you need: Equipment (for the experimental procedure using the BASIC-UNIT) Cobra3 BASIC-UNIT, USB 12150.50 Power supply, 12 V 12151.99 Software Cobra3 - Pressure 14510.61 Measuring module, pressure 12103.00 Support clamp for small case 02043.10 Support base, variable 02001.00 Support rod, l = 500 mm, stainless steel 02032.00 Bosshead 02043.00 Universal clamp 37715.00 Magnetic stirrer, mini 47334.93 Magnetic stirrer bar, l = 50 mm 46299.03 Erlenmeyer flask, narrow neck, PN 29 36424.00 Rubber stopper, d = 32/26 mm, 1 hole 39258.01 Glass tubes, straight, 80 mm, d = 7 mm, 10 pieces 36701.65 39282.00 Rubber tubing, di = 6 mm, 1 m Graduated cylinder, 100 ml 36629.00 Mortar with pestle, 150 ml, porcelain 32604.00 Strainer, fine mesh, d = 60 mm 40968.00 Graduated pipette, 1 ml 36595.00 Graduated pipette, 10 ml 36600.00 Glass beaker, 250 ml, tall form, DURAN 36004.00 Tripod, ring d = 100 mm, h = 180 mm 33299.00 Wire gauze, 120 x 120 ml 33287.03 Bunsen burner, natural gas 32165.05 Safety gas tubing, 1 m 39281.10 Tubing clip, d = 8...12 mm 40996.01 Test tube, 100 mm, d = 12 mm, FIOLAX, 100 pieces 36307.10 Glycerol, 99%, 100 ml 30084.10 Dropping bottle, plastic, 50 ml 33920.00 Hydrogen peroxide, 30%, 250 ml 31710.25 Hydrochloric acid, 1 molar, 500 ml 48454.50 Sodium hydroxide, 1 molar, 500 ml 48329.50 Ice cubes, Chicken liver or pig liver Distilled water PC, Windows® 95 or higher
The enzymatic activity of catalase
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 1 1 1 1 2 1 1 1 1 1 1
Normal result
Result when acidic
P4120611
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Laboratory Experiments, Biology 75
Handbooks Computer assisted Experiments with Cobra3 Physics, Chemistry / Biology Cobra3 Chemistry/Biology • No. 01320.02 61 described Experiments Please ask for a complete equipment list Ref. No. 25423
Cobra3 Physics • No. 01310.02 84 described Experiments Please ask for a complete equipment list Ref. No. 25422 1. Mechanics 1.1 (13301) Frequency of a spring pendulum 1.2 (13302) Frequency of a thread pendulum 1.3 (13369) Free fall with a screen 1.4 (13370) The path-time law for free fall with the falling sphere apparatus 1.5 (13371) Uniform, linear, unaccelerated motion 1.6 (13372) Force-free, linear motion 1.7 (13373) Uniformly accelerated, linear motion, Newton`s 2nd law 1.8 (13374) The elastic collision 1.9 (13375) The inelastic collision 1.10 (13376) Frequency of a thread pendulum 1.11 (13377) Pendulum oscillationsvariable g pendulum 1.12 (13378) The harmonic oscillation of helical springs-parallel and series connection of spring 1.13 (13379) Dependence of the oscillation period of a leaf spring on the pendulum length & pendulum mass 1.14 (13380) Moment of inertia of different bodies:disc,solid and hollow cylinder - Steiner`s law 1.15 (13381) Uniform rotary motion 1.16 (13382) Uniform, accelerated rotational motion, moment of inertia 2. Acoustics 2.1 (13360) Measurement of frequency of tuning forks, detuning of tuning forks 2.2 (13361) Investigation of the natural oscillation of columns of air 2.3 (13362) Measurement of the speed of sound in air 2.4 (13363) Measurement of the speed of sound in diffent gases 2.5 (13364) Measurement of the speed in metal rods 2.6 (13365) Acoustical Doppler effect 2.7 (13615) Investigation of beats 2.8 (13619) Influence of damping on the spectrum of the characteristic oscillations of air columns 2.9 (13620) Characteristic oscillations in cavity resonators - Hemholtz's resonators 2.10 (13621) Tone analysis
2.11 (13622) Oscillations in metal plates 2.12 (13623) Speech analysis 2.13 (13628) Signal filtration - elimination of background noises 2.14 (13629) Determination of the speed of sound in air - travel time measurement between two microphones 2.15 (13630) Acoustic spacial orientation 3. Thermodynamics 3.1 (13491) Specific heat capacity of water 3.2 (13492) Specific evaporation heat of water 3.3 (13493) Specific heat capacity of liquids 3.4 (13494) Specific heat capacity of solid bodies 3.5 (13495) Specific condensation heat of water 3.6 (13496) Specific melting heat of ice 3.7 (13497) Specific dissolution heat of salts 3.8 (13498) Specific heat value of fuels 3.9 (13499) Volume expansion of gases 3.10 (13500) Gay-Lussas Law 3.11 (13501) Amontons Law 3.12 (13502) Boyle's and Mariotte's Law 3.13 (13641) Gay-Lussac's law 3.14 (13642) Charles' (Amontons') law variant A 3.15 (13643) Charles' (Amontons') law variant B 3.16 (13644) Boyle and Mariotte's law 4 Electricity 4.1 (13303) Ohm's Law 4.2 (13304) Temperature dependence of the resistance of pure metals 4.3 (13305) Characteristics of semi-conductor diodes 4.4 (13306) Collector current-collector voltage characteristic of an NPN-transistor 4.5 (13307) Second order conductors 4.6 (13308) Switch-on current of a incandescent bulb 4.7 (13309) Measurement of the work and power of an incandescent bulb 4.8 (13310) Switch-on behaviour of a capacitance 4.9 (13311) Switch-on behaviour of an inductivity 4.10 (13312) Induction impulse
76 Laboratory Experiments, Biology
4.11 (13313) Generation of an alternating current, rectification and filtration 4.12 (13314) Efficiency of motor and generator 4.13 (13366) Measurement of the rebound time of a switch 4.14 (13367) Current-voltage characteristic of a solar cell 4.15 (13611) Single-valued and multiplevalued Fourier spectra 4.16 (13612) Analysis of simple and composite sinusoidal signals 4.17 (13613) Spectral analysis of different signal forms - sinusoidal, rectangular, triangular signals 4.18 (13614) Spectral analysis of periodic spike pulses 4.19 (13616) Determination of the non-linear distortion factor from the Fourier spectrum of distorted sinusoidal oscillations 4.20 (13617) The Fourier spectrum of rectified, non-smoothed alternating currents 4.21 (13618) Investigation of the characteristic oscillations of air columns 4.22 (13624) Coupled electrical resonant circuits 4.23 (13625) Forced oscillations of a non-linear electrical series resonance circuit - chaotic oscillation 4.24 (13626) Analysis of Fourier series 4.25 (13627) High-pass, low-pass, bandpass filters 4.26 (13631) Switch rebound 4.27 (13632) Phase relationships in a series resonant circuit 4.28 (13633) Free damped oscillation 5. Optics 5.1 (13315) Dependence of the luminous intensity on the distance 6. Physical Structure of Matter 6.1 (13368) Franck-Hertz Experiment 6.2 (13634) Range of alpha particles in the air 6.3 (13635) Mean range of beta radiation in air 6.4 (13636) Absorption of electrons (or positrons) in thin layers of matter 6.5 (13637) The quantal flux of gamma radiation in air 6.6 (13638) Absorption of gamma quanta (or electrons) as a function of material density 6.7 (13639) Law of radioactive decay 6.8 (13640) Radioactive equilibrium
1. Food Chemistry 1.1 (13705) The manual titration of citric acid 1.2 (13706) The automatic titration of ordinary vinegar 1.3 (13707) Determination of the phosporic acid content of a soft drink 1.4 (13708) The pH and degree of acidity of coffee 1.5 (13709) Determination of the content of fruit acid in juices and wine 1.6 (13710) Titration curves of fresh milk and sour milk 1.7 (13711) Changes in pH during the aging of milk (souring) 1.8 (13712) The buffering properties of foods 1.9 (13713) Determination of the calcium carbonate content of egg shell 1.10 (13714) Determination of the common salt content of meat broth 1.11 (13715) Chloride in mineral water 2 Ecology and Environment 2.1 (13701) The conductivity of various water samples 2.2 (13702) The pH of various water samples 2.3 (13703) The origin of acid rain 2.4 (13704) The twenty-four hour rhythm of an aquatic plant 2.5 (13509) Comparison of the heat capacities of water and land 2.6 (13510) The Bergman rule: heat loss in dependence on body surface area and volume 2.7 (13511) The isolating effect of body coverage 2.8 (13325) Daily course of luminosity 2.9 (13325) Daily course of luminosity 3. Biochemistry 3.1 (13696) Determination of the isoelectric point of an amino acid (glycine) 3.2 (13697) The ionic permeability of the cell membrane 3.3 (13698) Determination of the Michaelis constant 3.4 (13699) Substrate inhibition of enzymes 3.5 (13700) Enzyme inhibition (poisoning of enzymes) 4. Neurophysiology 4.1 (13600) Neuro-simulator (membrane time constant and low-pass filtering) 4.2 (13601) Neuro-simulator (how an exciting synapse functions) 4.3 (13602) Mechanical stimulation of the rear end of an earthworm 4.4 (13603) Mechanical stimulation of the front end of an earthworm 4.5 (13604) Electrical stimulation of an anaesthesized earthworm
5. Human Physiology 5.1 (13326) Cardiac and vasular sonic measurement (Phonocardiography) 5.2 (13327) Electrocardiography 5.3 (13327) Electrocardiography 5.4 (13503) Electromyography 5.5 (13605) Muscle stretch reflex and determination of conducting velocity 5.6 (13504) Electro-oculography 5.7 (13326) Cardiac and vascular sonic measurement (Phonocardiography) 5.8 (13606) Acoustic orientation in space 5.9 (13607) The enzymatic activity of catalase 5.10 (13505) Blood pressure measurement 5.11 (13506) Measurement of the respiratory rate 5.12 (13507) Changes in the blood flow during smoking 5.13 (13508) Regulation of body temperature 6. Plant Physiology 6.1 (13608) Photosynthesis (bubble-counting method) 6.2 (13513) Photosynthesis (measurement of oxygen pressure) 6.3 (13512) Transpiration of leaves 6.4 (13609) Glycolysis (measurement of pressure) 6.5 (13514) Glycolysis (measurement of temperature 6.6 (13515) Calorimetry of foods 7. Electrochemistry 7.1 (13318) Electrolysis of copper sulphate solutions 7.2 (13319) The electrochemical series of metals 7.3 (13320) Electric potential of a concentration element 8. Chemical equilibrium 8.1 Chromatographic separation processes: gas chromatography
(13321)
9. Gas laws 9.1 (13499) Volume expansion of gases (with Software Pressure) 9.2 (13500) Gay-Lussas Law (with Software Pressure) 9.3 (13501) Amontons Law (with Software Pressure) 9.4 (13502) Boyle's and Mariotte's Law (with Software Pressure) 9.5 (13641) Gay-Lussac's law (with Software Gas Laws) 9.6 (13642) Charles' (Amontons') law - variant A (with Software Gas Laws) 9.7 (13643) Charles' (Amontons') law - variant B (with Software Gas Laws) 9.8 (13644) Boyle and Mariotte's law (with Software Gas Laws)
PHYWE Systeme GmbH & Co. KG · D - 37070 Göttingen
Index
A
Colour saturation
43
Complementary colour
43
Conducting veIocity
23
F
23
Conductivity
58
Facilitation
Acidic anions
70
Conductivity of water samples
58
Acid rain
60
Acoustic hearing thresholds Acoustic orientation
Achilles tendon reflex
Action potential
23
Insulating effect
Feedback reaction
41
Intracellular potential
Field of view
39
Ionic permeability
71
26
Finger labyrinth
48
Isoelectric point
70
28
Fixation period
42
Flapping sound
17
Flicker fusion frequency
40
Frequency differentiation
27
8, 9, 10, 13
Additive colour mixing
43
Air pollution
60
Aquarium water
I
58, 59
Artificial cell membrane
71
Atria
16
AV nodes
16
D Daily course of luminosity
57
Dark reaction
61
Daylight reaction
61
Dead time
41
Dialysis tube
71
Diaphragmatic respiration
51
Diastole
B
17, 18
Diffusion potential
11
Diurnal rhythm
61
Drinking water
58, 59
Frequency modulation
8
35
Geotaxis
30
Geotropism
30, 31 34
Germination
Behaviour of mice
46
Glycolysis
Bergman rule
55
Gravity
31
Growth
34
Black masking
43
Blind spot
39
Blood flow
19
Blood pressure measurement
18
Blood pulse waves
18
Bodenstein principle
72
Body temperature
36
Breathing
50
Bubble counting method
64
Buffering effect
59
C
E Earthworm experiment chamber
16
ECG segments
16
H
Electrical stimulation
10
H+
Electrocardiogram
16
Hearing range
26, 27
Hearing threshold
26, 27
Electrophysiology Electrolytes Electromyogram
16, 22, 23, 42 58 22, 23
54
48
Learning performance
48
Low pass filtering
12
Luminosity
57
Lux measurement
64
M Marking behaviour
12
Merging frequency
26
Michaelis constant
72
EMG
22
High labyrinth
47
Enzyme
73
Human body temperature
Enzyme inhibition
74
Human electrocardiography (ECG)
16
Human electrooculography (EOG)
42
Human eye
40
Human merging frequency
26
Human reaction capacity
41
Human respiratory rate
51
N
42
Eupnea
51
Centrifugal force
31
Evaporation
36
Clinostat
30
Excitatory postsynaptic potential (EPSP) 13
Mineral water Motor piston burette Muscle Muscle contractions Muscle potential Muscle stretch reflex
Exciting synapse
13
Human visual field
39
Nerve
Eye movements
42
Humidity
66
Neurosimulator
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
11, 71
Membrane time constant
54
36
47
Membrane
Heat radiation
EOG
43
Heat capacity
Learning curve
42
71
Colour mixing
16
47, 48 48
Electrooculography
75
43
Heart rate
59
Learning behaviour
55
Cell membrane
Colour masking
71
47, 48
Learning
Heat loss
72, 73 74, 75
23
Lake water
22
Catalase
67
ions
Jendrassik effect
EIectromyography
54
CO2 pressure measurement
67, 68
ECG
Enzyme reaction
Capacities
8 9, 10
J Labyrinth
Geographical separation
70
8, 9, 10 16, 22, 23, 42
12, 13
L
G
Basic cations
Bio-amplifier
56
Nocturnal rhythm
58, 59 70 8 22 8, 9, 10, 22 23
8 12, 13, 16 61
Laboratory Experiments, Biology 77
Index
O
Q
O2 pressure
65
-
Q10 value
OH ions
71
Operative conditioning
47
Optomotor response
38
Orientation
28
R
Osmotic processes
71
Rainwater
Oxygen bubble counting
64
Rapid reading techniques
P PCG
17
Perimeter
39, 40
Permeability
71
pH
59, 61
50
34, 35
Waste water
Temperature preferendum
34, 35
Water
35
Thigmotaxis
35
42
Thoracic respiration
51
Reaction capacity
41
Threshold
Reflex
23
Threshold frequency
Reflex latency
23
Time resolving capability
Refractory period
10
Titration
70
Respiration energy
68
Torpor
35
Respiratory frequency
51
Transpiration
66
Transpiration of leaves
66
Twenty-four hour rhythm
61
58, 59
Respiratory rate
50, 51
Respirometer
50
Resting potential
11 61
pH of water samples
59
River water
Phonocardiography (PCG)
17
Photoperiodism
30
Photosynthesis
61, 64, 65
58, 59
Phototropism
30
S
pKs value
70
Sacchades
42
Plant germination
34
Scotoma
39
Platform for behaviour observation
46
Sine wave generator
Preferential temperature
35
Skin temperature
Pressure
67
Smoking
19
Pressure measurement
65
Soil temperature
34
Pulse
17
Spatial orientation
28
Ventricles
16
Visual field
39
30, 31 10 31, 38, 41
Subtractive colour mixing
43
58 58, 59 43
47
Z
11
19, 36
73
Systole
Y-Labyrinth
72, 73, 74
V
11, 71
Substrate inhibition
Synaptic facilitation
38, 40
26, 27, 40
Stimuli generator
Synaptic depression
41
46
Y
U Ussing chamber
Statoliths
Strobe drum
26, 27
White masking
Zwitterions
Urease
Selective permeability
Wall contact searching
Temperature organ
Thermotaxis
Rhythm
78 Laboratory Experiments, Biology
68
36
61
30, 35
Temperature
Temperature regulation
pH measurement
Phototaxis
W
T
9 9 17, 18
PHYWE Systeme GmbH & Co. KG 路 D - 37070 G枚ttingen
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