New Products

Liquid chromatography & mass spectrometry

Thermo Fisher Scientific Inc. has demonstrated the advanced analytical capabilities delivered by combining its Exactive liquid chromatography/mass spectrometry (LC/MS) system with its Transcend technology for a complete LC/MS solution. For challenging screening applications, including complex samples containing pesticides, metabolites or other target compounds, this configuration combines automated online sample preparation for high throughput with the dual benefits of ultra-high resolution and accurate mass. The Exactive delivers ultra-high resolution and accurate mass data to enable the rigorous and rapid characterization of compounds in complex matrices. The easy-to-use instrument streamlines many technical steps that normally require specialized setup and operation, and provides simultaneous qualitative and quantitative results. In addition, Thermo Scientific’s TurboFlow technology within Transcend offers automated, online sample preparation methodologies to simplify analytical workflows and increase data quality.

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360 µm High Pressure Fittings

VICI Valco’s new 360 μm High Pressure Fitting design permits direct connection of 360 micron OD fused silica, PEEK, stainless or electroformed nickel tubing without having to use troublesome liners. Because of the fitting’s compact size and fine two to 56 threads, a leak-free connection that seals at pressures well in excess of 20,000 psi can be easily formed with the available manual tool. The 360 μm fittings are available with a PEEK or stainless nut. The PEEK nut is supplied with a glassfilled PEEK ferrule, while the stainless nut uses a 316 SS ferrule. The ferrule snaps into the nut so that the fitting is ‘one-piece,’ but the ferrule is free to rotate and does not twist the tube as the nut is tightened. A wide range of micron bore sizes is available.

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Water Purification

Millipore Corporation has released its ICW-3000 water purification system. The new system produces a constant and reliable source of ultrapure water for Dionex IC (Ion Chromatography) systems. Ultrapure water from the ICW-3000 system can increase the precision of IC analyses. The ICW-3000 system provides a continual flow of ultrapure water to the eluent generator and regenerant lines of the suppressor. This reduces the carbonate content in the water – sharply decreasing contamination risks and improving experiment reproducibility, while also eliminating the need for helium. The water system also eliminates the need for eluent preparation and the instrument downtime that occurs when filling eluent bottles. With only two tubes and one power cord to connect, the new system is designed for plug-and-play installation, as well as everyday use. The user fills the system’s 10 L tank with pure water in order to produce a constant flow of ultrapure water for the IC system. The ICW-3000 system is remotecontrolled by the Dionex IC system: both systems are 100 per cent compatible.

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Data Logger

The Simple Logger® II Model L481 bipolar 850VDC data logger gives a choice of data storage modes and storage rates. This allows the operator to configure the logger to optimize memory usage to the application at hand. Extended Recording Mode (XRM®) and delayed start time are two of the many application features in this logger. Its internal memory allows storing over 240,000 measurements. User programmable scale and engineering units are available. Battery operation and compact size allows for installation in tight locations without the need for external power. A series of front panel LEDs provides a quick status of the logger’s state and memory usage. The Simple Logger® II Model L481 records DC voltage at user-selectable rates from eight every second to one every day. Voltages can be measured up to +850 VDC with 0.1 V resolution. DataView® software facilitates real-time viewing of measurement data even while recording.

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Sterilisation Cabinet

The GLE-UVSC UV sterilisation cabinet provides effective decontamination of reagents and equipment before carrying out sensitive PCR reactions, particularly when amplifying DNA sequences which are either in limited supply or of low copy number. Four timer-controlled 15-Watt UV-C bulbs enable the user to control the exposure time and dose of high energy UV-C irradiation required to denature nucleic acids that cause unwanted background contamination, while a timer override switch allows the UV source to remain on indefinitely if preferred. Safety interlocks switch off the UV-C bulbs automatically when the cabinet side doors are opened, preventing accidental exposure to the UV source. The cabinet is constructed from UV-C-impermeable 10mm acrylic, which also serves as an effective barrier against radioactive isotopes, allowing the user to work in complete safety with high energy ß-emitters such as 32P. A single white light bulb illuminates the work area when the cabinet is in use.

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Cloud Computing Application

The new version of Cresset’s FieldStere bioisostere generator delivers shorter lead optimization cycles to computational and medicinal chemists by enabling them to choose the best syntheses, to fine-tune portions of lead molecules and to scaffold hop to expand into new areas of chemical space. FieldStere V2.0 is the first in Cresset’s new generation of ‘cloud enabled’ applications. This brings real-time assessment of new directions for medicinal chemistry, patentability and in-licensing potential of drug candidates a step closer. FieldStere can also be run as a traditional desktop application, allowing users without a large computing infrastructure to benefit from the same functionality. FieldStere V2.0 introduces more flexible licensing and new features to improve productivity. A command line interface supports scripting and workflow systems, while FieldStere databases now have automatic fragment de-duplication and time-stamping to support results tracking in ELN & patent applications. FieldStere also enables results to be exported as PDFs and copied in 2D or 3D for pasting into drawing applications or presentations.

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NEW PRODUCTS

Chiral Screening

Phenomenex Inc. announces a Chiral Screening Service for customers in pharmaceutical and natural products research and development. This free service provides target screening using a library of HPLC and SFC columns including the Phenomenex Lux™ polysaccharide-based offerings, which demonstrate a success rate approaching 90 per cent. After resolving the chiral compound, Phenomenex also produces method development and optimization for the customer within 10 business days. Enantiomers of chiral compounds may have different pharmacological effects in biological systems. Regulations require that enantiomers of all chiral drugs in development be screened separately on their pharmacodynamic and pharmacokinetic properties. The Phenomenex team of chiral experts will customize a plan for each screening project, taking into account specific requirements and overall separation goals. Once a project is completed, the customer will receive a report that details the optimal methodincluding information on the chiral stationary phase (CSP) selected for the separation, running conditions and associated chromatograms.

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High performance liquid chromatography system

Eksigent, has unveiled the ExpressLC-Ultra. This new microscale HPLC system runs at column pressures up to 10,000 psi, allowing the use of particle columns smaller than two microns. Along with a new UV detection system, the system delivers all the traits of microscale LC, including solvent savings, reduced sample requirements and reduced frictional heating. The new system’s CCDbased UV detector works with microfabricated flow cells to ensure ultra-high sensitivity with no band broadening. The ExpressLC-Ultra’s high UV sensitivity allows detection of impurities at levels as low as 0.05 per cent, meeting the needs of researchers in drug discovery and other applications. The system incorporates Eksigent’s proprietary Microfluidic Flow Control technology to generate accurate and reproducible flow rates, from one to 50 microliters per minute. New features make the ExpressLC-Ultra as easy to use as any conventional HPLC instrument. The ExpressLC-Ultra includes Eksigent’s control software with specialized diagnostic tools.

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Sensor Chip

For screening and characterization of small molecules and fragments in Biacore™ SPR systems, GE Healthcare announces the launch of Sensor Chip CM7. Designed for use with GE Healthcare’s Biacore™ systems, Sensor Chip CM7 has the high immobilization capacity required to support fragment-based screening of small molecules. Sensor Chip CM7 offers improved screening data quality as it delivers high signal-to-noise ratios. In addition, Sensor Chip CM7 gives improved detection in cases where immobilization levels are unfavourable, such as low concentration of target protein or a target protein that is sensitive to optimal immobilization conditions. Sensor Chip CM7 has a carboxymethylated dextran matrix covalently attached to gold film, with a high degree of carboxylation and a denser matrix compared to Sensor Chip CM5. It is capable of binding proteins, nucleic acids, carbohydrates or small molecules to carboxyl groups on the sensor surface via –NH2, -SH, -CHO, -OH, or -COOH. The analyte is then passed over the surface and any binding is detected by the Biacore system.

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Magnetic Stirring Bars

Magnetic stirring bars, used to stir and mix everything from solutions in a cuvette to 55 gallon drums are standard equipment in many laboratory and manufacturing procedures. Traditionally covered in PTFE, the durability of magnetic stirring bars can be cause for concern in many applications including the mixing of granular slurries or when extended stirring times are required. Bel-Art Products has introduced Spinfinity™, a magnetic stirring bar that addresses these durability concerns and more. The Spinfinity™ magnetic stirring bar is an octagon shape with a built-in pivot ring. This shape provides a greater surface area than a rounded bar and the interrupted profile of the pivot ring reduces friction and chattering. In the lab, they can quickly be identified from other magnetic stirring bars by their distinctive purple color and are 3” x ½” in size.

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Mill for sample preparation

Retsch’s GM 300 is the newest mill for sample preparation of foodstuffs. With a capacity of 4.5 litres and a 1.1 kW motor, the GM 300 quickly and efficiently homogenizes samples with high water, oil or fat content. Its special knife design and variety of grinding chambers allows for the homogenization of large quantities of frozen french fries, pizza and gummy candies. Simple to clean, the grinding chamber and knife are easily attached and removed without the use of tools. When the grinding cycle is complete, the container can be lifted from its base and replaced with a clean one; eliminating cross contamination between samples. In addition, all components that come in contact with the sample are removable and autoclavable. With speed parameters from 500 – 5000 min-1, the mill can be properly adjusted for preliminary or fine size reduction. A digital display can store up to 10 parameters combinations, ensuring reproducible results regardless of the user.

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FEATURE

Continued from page 9

by GC/MS. As with the LC/MS evaluation the vials were exposed at room temperature and 50°C. There was no significant difference between the room temperature and elevated temperature test results. A typical GC/MS chromatogram and mass scan are shown in figures 3 and 4 using positive electrospray ionization, with blank solvent in the upper scan and the vial extract shown in the lower scan.

Conclusion

In order to achieve improved levels of sensitivity, reliability, and reproducibility, today’s high performance hyphenated techniques require the elimination of all possible contaminants from every stage. The sample vial is an important link between the prepared sample and final analysis, and as such must also meet this requirement.

Vials must, therefore, be produced under the strictest automated manufacturing and quality controls systems to ensure a reproducible performance is obtained with every use. Studies have shown that the new National Scientific Mass Spec Certified vials exhibit minimal background particulate levels and minimal LC/MS and GC/ MS background contamination over a wide variety of conditions. Consequently, these new pre-cleaned vials are highly effective and offer the reassurance of meeting the rigorous demands of an extensive range of current highly sensitive chromatography applications.

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COMPANY & ADVERTISER INDEX

COMPANY PAGE RC

AEMC.............................................14................4344 AEterna Zentaris Inc. ........................7......................... Allon Therapeutics Inc. ......................3......................... Bel-Art Products .............................15................4350 BIOTECanada.............................................................6 BUCHI Corporation.........................19................4352 Caledon Laboratory Services ..........5.................4336 Canada Foundation for Innovation...1, 3, 6..................... Chemical Institute of Canada ..................................... and the Canadian Society for ..................................... Chemical Technology.......................4.................4335 Children’s Miracle Network............6.................4337 Cresset ..............................................................4346 Cynapsus Therapeutics Inc.................7......................... Dana-Farber Cancer Institute ............7......................... DiagnoCure Inc.................................6......................... Eksigent..........................................15................4348 Fisher Scientific..............................2.................4334 GE Healthcare.................................15................4349 Genome Canada..............................1,2....................... Hamilton Thorne Ltd..........................6......................... Immunovaccine Inc............................7......................... Keryx Biopharmaceuticals..................7......................... Millipore Corporation .................14, 30...4343, 4353 Medicure Inc....................................6......................... National Research Council of Canada .3......................... OncoGenex Pharmaceuticals Inc.......4, 6....................... Ontario Ministry of ...................................................... Research and Innovation ...................1......................... Paladin Labs Inc................................7......................... Phenomenex Inc. .............................15................4347 POI ................................................13................4340 Retsch ...........................................15................4351 Scie-Plas Ltd...................................14................4345 Stellar Pharmaceuticals ....................6......................... Stem Cell Therapeutics Corp..............7......................... Thermo Fisher Scienitific ...............10, 14.............4341 Trent University................................3......................... VICI Valco........................................15................4342 VWR ..............................................11................4339 Wyvern Scientific Inc......................7.................4338 YM BioSciences Inc...........................7.........................