Journal of Research in Ecology
Journal of Research in Ecology
ISSN No: Print: 2319 –1546; Online: 2319– 1554
An International Scientific Research Journal
Short Communication
Isolation and identification of blood parasites of quail in Baghdad area Authors: Suhad Y. Jassim and Hind Dyia Hadi
ABSTRACT: 20 specimens of the quail bird belonging to the family Phasianidae were collected from different and random areas of Baghdad city (central Iraq) for the period between February-May 2017 were examined for blood parasite. Only one genus, the Plasmodium sp. was identified in 10 samples with 50% infection rate.
Institution: Iraq Natural History Research Center and Keywords: Museum, Bab Al-Muadham, Blood parasites, Plasmodium, Quail, Middle of Iraq. University of Baghdad, Iraq. Corresponding author: Suhad Y. Jassim
Article Citation: Suhad Y. Jassim and Hind Dyia Hadi Isolation and identification of blood parasites of quail in Baghdad area Journal of Research in Ecology (2018) 6(1): 1589-1592 Dates: Received: 28 Feb 2018 Accepted: 16 March 2018 Published: 27 April 2018 Web Address: http://ecologyresearch.info/ documents/EC0548.pdf Journal of Research in Ecology An International Scientific Research Journal
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1589-1592 | JRE | 2018 | Vol 6 | No 1
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Jassim and Hadi, 2018 from February to May 2017 were caught alive by the
INTRODUCTION According to the fourth national report of the
bird hunting nets. The birds were ethically handled and
convention on biological diversity, it has been con-
brought to the laboratory, blood was obtained by mak-
firmed that the list of Iraqi birds includes 417 bird spe-
ing a small wound in the wing vein (peripheral blood)
cies, 118 of them are migratory birds (Anonymous,
and without harming the bird, a thin smear is done and
2010), among these birds is the quail Coturnix coturnix
left to dry, then the smear is fixed by absolute methanol,
which belongs to the order of Galliformes and Phasiani-
stained with Giemsa's stain for an hour, wash with tap
dae family which are heavily deployed in Iraqi territory.
water and leave to dry, the blood film is then examined
Quail is a bird used in productive-economic projects, its
by a compound microscope on the oil lens (100x) to
size is small with long wings and short tail and its origi-
check for parasites and identification of the types, the
nal home is Japan (Mahdi and Georg, 1969; Ghani et
slide is imaged using digital camera (Infinity lite-K 100)
al., 2008). The quail is a wild bird that can migrate and
attached to the compound microscope (Micros MCX
fly from place to place and then return to its homeland
100) (Bennett and Campbell, 1972; Mohammad, 1991).
(Shanaway, 1994). Studies on the blood parasites of birds, especially quail, were very few in relation to the
RESULTS AND DISCUSSION
large diversity of bird populations in the local and for-
The bird used in the current study is quail and,
eign environment (Jasim, 2006; Youssef and Mansour,
as mentioned previously, it is a migratory bird and
2014). Among those studies (Shamsuddin and Moham-
widely present in Iraq. As a result of this work, only one
mad, 1981) which is considered one of the first studies
of the protozoan parasites genus; Plasmodium sp
that concern the parasites of blood in Iraqi birds, many
(Figure 1 a and b) was found in ten of the samples from
studies are done as follows Mohammad, (1990, 1991
a total of 20 birds samples of quail with an infection rate
and 1996), (Mohammad, 2012; Islam et al., 2013; Bahar
of 50%, this result is consistent with the findings of
et al., 2014; Peterson, 2007; Bradley et al., 2004). The
(Mohammed, 2012) but the infection rate was very low
aim of this work is to isolate and diagnose the parasites
compared to the current study, (Mohammad et al.,
that affect the quail blood located in the city of Baghdad
2001) also, the percentage of infection was very low,
(central of Iraq).
Shamsuddin and Mohammad, (1981) recorded the presence of plasmodium in addition to two new species-first recorded in Iraq but with lower infection when they
MATERIALS AND METHODS Adult birds (20) of quail were collected from
studied blood parasites in Iraqi birds, Shaapan et al.
different areas of Baghdad governorate (center of Iraq)
(2011) found Cryptosporidium sp in fecal and serum
a
b Figure 1. Plasmodium sp
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Journal of Research in Ecology (2018) 6(1): 1589-1592
Jassim and Hadi, 2018 samples from native quails. This indicates that
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ACKNOWLEDGEMENTS I extend my thanks and appreciation to everyone who contributed to this work. I especially mention my professor Mohammad K. Mohammad who has been credited with diagnosing parasites. I am also thankful to
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Journal of Research in Ecology (2018) 6(1): 1589-1592