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COST Action FA 1101. Saffronomics WG2 Interim meeting on Phytochemistry and Metabolomics and WG1/WG3/WG4 Looking for Synergy and Dissemination Capacity Building Place: Chania (Crete, Greece), Mediterranean Agronomic Institute of Chania, Conference Center

AGENDA Date: Thursday, 30 May 2013 9:30-9:45

Welcome and opening of meeting by the Local Organizer (Prof. M. Z. Tsimidou, AUTh) Session 1: Project management Chairwoman: Prof. M. Z. Tsimidou

9:45-10:00 10:00-10:30 10:30-11:00 11:00-13:00 13:00-14:30

Cost Officer to be confirmed Progress of COST Action FA1101 by Dr. Marta Roldan Medina, Grant Holder and Maria Tsimidou, Chair Coffee break Visit to the Mediterranean Plant Conservation Unit of MAICh (guidance by Dr. Chr. Fournaraki, curator) Light Lunch (offered by the local organizer)

Session 2:Advances in Phytochemistry and Metabolomics of saffron. Focus on extraction and isolation techniques (WG2) Chairpersons: Dr. N. Nenadis, Dr. A. Trapero 14:30-15:00

Kinetic studies of non polar organic extracts of Crocus sativus L. stigmas E. Anastasaki, K. Venetsanou, Spyridon Daousis, P. Tarantilis, M. Polissiou

15:00-15:30

Comparative study of ultrasound and magnetic stirring assisted extraction for the preparation of saffron polar extracts A. Kyriakoudi, F. Mantzouridou, M.Z. Tsimidou Comparative study of classical and ultrasound-assisted extraction of nonvolatile compounds of Crocus sp. C. Mitsi, D. Daferera, E. Anastasaki, C. Kanakis, K. Astraka, P. A. Tarantilis, M. Polissiou Coffee break

15:30-16:00

16:00-16:45


Session 3: Advances in Phytochemistry and Metabolomics of saffron. The diagnostic potential of spectroscopic techniques Chairman: Prof. M. Polissiou 16:45-17:45

Invited lecture: ESI-MS applications to flavonoid and terpenoid metabolomics. Cases of diagnostic fragments by Dr. Panagiotis Kefalas, Director of Laboratory of Chemistry of Natural Products and Analytical Chemistry of MAICh

17:45-18:15

Identification of safranal in essential oil from saffron using Infrared, Raman, 1H and 13C Nuclear Magnetic Resonance Spectroscopy P.A. Tarantilis, C.S. Pappas, E. Petrakis M.G. Polissiou

Date: Friday,31 May 2013 Session 4: Advances in Phytochemistry and Metabolomics of saffron. Thediagnostic potential of spectroscopic techniques and chemometrics Chairman: Dr. R. Consonni 9:30-10:00

10:00-10:30 10:30-11:00

11:00-11:30

11:30–13:00 13:00-14:30

Monitoring saffron ageing by PTR-MS measurement of volatiles N. Nenadis, S. Heenan, S. Van Ruth, M.Z. Tsimidou Coffee break Exploiting the diagnostic value of the fingerprint region in FT-IR spectra for the quality control of saffron S.A. Ordoudi, M.de los Mozos Pascual, M.Z. Tsimidou Preliminary results on the discrimination between aged Greek and Iranian saffron samples by 1H-NMR and chemometrics E. Naziri, L.R Cagliani, S.A. Ordoudi, M.Z. Tsimidou, R. Consonni Visit to the MAICh accredited laboratories for natural products analysis Light lunch

Session 5: Advances in Phytochemistry and Metabolomics of C. sativus and allies. Synergy between WG2 and WG1/WG3 Chairpersons: Dr. S. Fluch, Dr. G. Giuliano 14:30-15:00

15:00-15:30

15:30-16:00

In planta and in bacterio functional characterization of the putative Zeaxanthin Cleavage Deoxygenase from saffron. G. Diretto, A. Garcia, S. Frusciante, M. Pietrella, P. Ferrante, S. Al-Babili, G. Giuliano Comprehensive metabolite profiling and transcriptome co-expression analysis leading to decoding glycosyltransferase-aglycone correlation in stigmas of Crocus sativus A. Trapero, M. Pietrella, A. Rubio-Moraga, G. Diretto, L. Gómez-Gómez and G. Giuliano Chemical and biological bioprospection of Crocus leaves extracts O. Santana, R. Sánchez Vioque, P. Tarantilis, K. Astraka, M. de los Mozos Pascual, M. Roldan, M. Polissiou


16:00-16:30

16:30-17:00 17:00-18:00

Characterization of different wild crocus species from areas of Cyclades andCrete Islands P. Ralli, C. Dordas Coffee break Invited lecture: The archaeology of the saffron by Dr. A. Sarpaki, archaeologist

Date: Saturday, 1 June 2013 Session 6: Expansion of saffron cultivation in Europe and dissemination activities Chairpersons: Prof. A. Mozzarelli, Dr. O. Santana 9:30–10:30

10:30-11:00 11:00-11:20

11:20-11:40 11:40-12:00

12:00-12:20

12:20-12:40

12:40-13:00 13:00-14:30

Invited lecture: NMR potential in Metabolomics of Natural Products by A. Spyros, Assistant Professor, Department of Chemistry, University of Crete Coffee break Establishment of a collection of saffron lines by micropropagation at the Walloon Agricultural Research Center of Gembloux (CRA-W Belgium) – First Evaluation G. Pascal, D. Martine, W. Bernard Revival of Crocus Austriacus B. Kaar Effects of post-harvest processing onchemical composition of saffron(Crocus sativus l.) M. Arapceska, Z. Jankuloski Synergies between the Italian saffron producers and Italian saffron researchers A. Mozzarelli, G. Paredi, S. Raboni Working with pupils of the high-school in Krokos (Kozani, Greece) on saffron production, processing, quality, traceability and authenticity aspects. K. Tsioga, E. Pouliou, A. Kyriakoudi, S.A. Ordoudi, M.Z. Tsimidou Chemical composition from different parts of Saffron (Crocus sativus l.) M. Arapceska, Z. Jankuloski Closing of the meeting


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