COST ACTION FA 1101 Omics Technologies for Crop Improvement, Traceability, Determination of Authenticity, Adulteration and Origin in Saffron. “Saffronomics� Training School
Spectroscopic and chemometric approaches to saffron analysis National Council of Research, Institute for Macromolecular Studies v. Bassini 15 Milan (ITALY) 27-29 May 2014 The main aim of this training school will be to present the advanced spectroscopic techniques applied to the quality characterization of saffron, in combination with chemometric. The basic principles and latest tools of NMR and other spectroscopic FT techniques will be also presented. A practical NMR section will be performed at Bruker laboratories, covering the NMR sample preparation, data acquisition, processing and chemometric evaluation of data. In addition, advanced multivariate statistical protocols applied to spectroscopic techniques will be presented in theoretical and practical sections. Up to 9 trainees will be reimbursed by COST office, and a maximum number of 15 participants is expected. To register please contact: roberto.consonni@ismac.cnr.it.
Training School Program 27th Tuesday (ISMAC at 8.30) • Needs in saffron analysis beyond ISO 3632 requirements. Prof. M. Tsimidou (Aristotele University of Thessaloniki)
• UV-Vis and fluorescence applications in saffron quality control and detection of adulteration. Prof. M. Tsimidou (Aristotele University of Thessaloniki)
• FT-IR and Raman Spectroscopy in the Geographical Origin and Adulteration of Saffron and Classification of various Crocus species.
• PCA (model building, diagnostics, interpretation) • PLS regression (model building, cross validation, permutation test, model interpretation) • OPLS (when to use, model interpretation) • (O)PLS discriminant analysis • Practical sessions • Discussion
29th Thursday (Bruker Biospin 9.00 -16.00)
Prof. M. Polissiou (Agricultural University of Athens)
• General applications of NMR to food matrices: highlights on saffron. Dr. R. Consonni (ISMAC CNR Milan)
13.30-16 out door section
28th Wednesday(ISMAC 9.00-18.00)
Practical NMR on food. Dr. F. Benevelli, A. Minoia, C. Napoli (Bruker Biospin lab)
• How to prepare NMR samples from food matrices • Experimental setup: temperature, solvent suppression, pulse calibration, pulse sequences • NMR experiments
Experimental setup in practice Chemometrics. Dr. M. Stocchero (S-IN Soluzioni Informatiche)
• Automatic processing of data
• Spectroscopic data processing (alignment, OSC filters, bucketing) • Multivariate data analysis: latent variables approaches
Concluding remarks