Transplante Uterino - Perspectivas Hist贸ria, Est铆mulo e Hierarquia
Marcos Pinotti
About me
Mechanical Engineer (M.Sc.; Ph.D.)
Professor
56 patents
Entrepreneur
Advisor
Founder 4 Companies
Top 100 Bioengineering Scientist (IUSBES) High Brain Tech Park Top 10% Mechanical Engineer Professor Brazil (CNPq 1B) VP Latin American Society for Biomaterials, Art. Organs and Tissue Engineering Brazil-American Frontiers of Engineering (BAC - National Academies)
Brazilian Presidential Office - Assistive Technology Committee Ministry of Science, Technology and Innovation State of Minas Gerais Belo Horizonte Tech Park
interfaces
Do you really believe that process will lead to a whole organ printing ?
Human Lego
What about scaffolds ?
Will it disappear after the job done ?
What about scaffolds ?
Which is the better pattern ?
Adding layers ?
How to obtain this hierarchy ? specialization ? longevity ?
Can we have prefabricated parts ?
How to connect them ?
What we know about tissue and organs ? Evolution made improvements of old structures Organs grow from the inside The correct sequence of stimuli leads to a shape and function There is a pre-programmed instructions on how to grow There is a balance between agonistic and anti agonistic actions
Why Whole Organ Decellularization ? Keep distribution and composition of extracellular matrix Keep 3D organ structure (blood vessels and ducts) Allows to repopulate cells in a bioreactor Extracellular matrix can be remodeled Matrix serves as a guide for differentiation and cell migration
Growing New Organs Using Stem Cells in a Scaffold Designed by Nature Donor Tissue (Valve, Heart, Lungs) Decellularization process
Repopulating the hollow tissue with recipient stem cells to grow new tissue or organ
Rat heart - University of Minnesota
Stem Cells
Rat Lung - University of Minnesota
Growing New Organs Using Stem Cells in a Scaffold Designed by Nature Donor Tissue (Valve, Heart, Lungs) Decellularization process
Repopulating the hollow tissue with recipient stem cells to grow new tissue or organ
Rat heart - University of Minnesota
Stem Cells
Rat Lung - University of Minnesota
Challenges Where - Bioreactor How - Immersion. Perfusion. Perfusion/Immersion When stop? Keep 3D structure without sites for host response
Before repopulation Mechanical tests (collagen and elastin)
Morphology (Trasmission Electron Microscopy) Immunological tests
How to use “human legos”
History
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Stimuli
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Hierarchy
History
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Stimuli
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Hierarchy
History
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Stimuli
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Hierarchy
History ?
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Stimuli ?
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Hierarchy ?
History ?
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Stimuli ?
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Hierarchy ?
History ?
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Stimuli ?
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Hierarchy ?
Challenges Where - Bioreactor - History
How - Cell insertion, mechanical/electrical/hormones/biochemical stimuli Sequence and pace of stimuli - Embryology path? - Hierarchy
Bioreactor
Transplantation
Bioreactor + In vivo Bioreactor
Thank you
lapan.pinotti@gmail.com