International Journal of Botany and Research (IJBR) ISSN (P): 2277–4815; ISSN (E): 2319–4456 Vol. 10, Issue 1, Jun 2020, 33–38 © TJPRC Pvt. Ltd.
PROSPECTS OF MICROPROPAGATION OF MORUS INDICA L. VAR. ROTUNDILOBA FROM NODAL EXPLANTS SUDIPTA KUMAR SIL1, NABANKUR MUKHERJEE2 & MOUMITA BASU3 1 2,3
Professor, Department of Botany, University of Gour Banga, Malda, West Bengal, India
Research Scholar, Department of Botany, University of Gour Banga, Malda, West Bengal, India
ABSTRACT Mulberry plants are economically very important for sericulture industry as it’s leaves are only food for silkworm Bombyx mori. Based on quantity and quality, there are different cultivating mulberry varieties in the world. In this study, In-vitro culture is practiced with nodal explants of mulberry variety Rotundiloba to produce large number of plantlets in short period of time. Nodal explants cultured in media having various concentration of BAP (N6- benzyladenine) and Kinetin either alone or in combination. Callogenesis were also tested using 2, 4-D. Culture media MS0+ 1.5 6-BAP +0.5 Kinetin mg/L and MS0+ 1.0 Kinetin mg/L produced highest % of microshoots and culture media MS0+ 1.5 6-BAP +1.5 Kinetin mg/L produced highest cumulative number of microshoots. Rooting induced in both culture media having 0.50 and 0.75 mg/L IAA concentration.
peat moss (1:1:2) under glasshouse condition. We can conclude that maybe this is the first report of in-vitro clonal propagation of Morus sp var. Rotundiloba through nodal explants by plant tissue culture technique. KEYWORDS: Mulberry, Tissue Culture, Callogenesis, Rotundiloba, Culture media, BAP, Kinetin & IAA
Received: Jan 13, 2020; Accepted: Feb 03, 2020; Published: Feb 24, 2020; Paper Id.: IJBRJUN20205
Original Article
Four week old micropropagated plantlets were transferred to plastic pots containing a mixture of soil: sand:
INTRODUCTION Morus commonly known as mulberry is a deciduous tree and it is under cultivation in many parts of the world specially in temperate regions. In China, there are more than 1000 varieties of mulberry varieties is under cultivation (Dutta, 2001). In India also many states adopted mulberry as a cultivating crop for sericulture industry. In China, mulberry fruits is used to treat prematurely grey hair. It is also used to treat diseases like constipation and diabetes. Tree barks used to treat headache, fever, sore eyes etc. Mulberry leaves are most important for sericulture as it is sole food of Bombyx mori or silkworm. Mulberry varieties are highly influenced in quantity and quality of leaves based on their cultivation practices, age and preservation techniques (Bongale et.al., 1997). Silk used commercially for making exceptional silk garments. 90% of the total global raw silk produced by mulberry varieties significantly helps to the of many people across the globe economically. Strong environmental adaptability demonstrated an encouraging result to stabilize the adverse effects of heavy metals in mulberry especially in the diverse polluted soils (Peng et al., 2012; Delplanque et. al., 2013; Zhou et al., 2015). It is found that mulberry leaves has a strong absorption ability of pollutants that helps in controlling of atmospheric pollution.
www.tjprc.org
editor@tjprc.org