CamTechCare Newsletter - October 2018

CamTechCare Newsletter

October | 2018

Keeping you informed In this Newsletter Directors Blog JWI Award Application 2019 Bronze Green Impact awards Mouse Handling Webinar Upcoming Events 2018 Plus much more‌..

Directors Blog As this is the October issue, Halloween is featured on the cover page in the Innes, I’m told the pumpkin fun became a bit messy later!

We clearly pulled out all the stops for British summertime to provide us with sunshine for the BBQ, glad so many of you could make it, Queens provided plenty of food and drink to keep us all happy well into the afternoon.

In this issue, you will see that Debs has highlighted the LASA Conference and IAT Congress calls for poster applications. We encourage you to consider this, there are people who are able to help you in UBS if you have not done one before and there is, I know a plethora of exciting new ideas from all facilities. If you are intending on applying to attend any meetings do so through your NACWO and consider applying for a bursary which will allow even more people to attend, see “Funding Opportunities”.

Datesand have launched the 2019 “Janet Wood Innovation Award” after previous years successes we’d love to hear and showcase more of your ideas, it’s an opportunity to make a difference to environmental refinement worldwide.

Finally we need to talk about Christmas, I know but it’s not far away, I’d like you to join us all at the University Centre on December 14th for festive food and fun.

Cover photo: Innes Marmosets enjoying Halloween

2 | CamTechCare Newsletter | October 2018

20 19

Datesand Ltd are proud to announce the launch of this potentially life changing Innovation Award.

2018 Winner The Numbrero

By Vanessa Jenkins

Name: Company/ Organisation:

Janet Wood Co-founder of Datesand 1944 - 2010

In association with: PARSEMUS FOUNDATION

CamTechCare Newsletter | October 2018 | 3

The Competition Thank you for downloading this application pack.

Janet Wood was the cofounder of Datesand Ltd and the mother of current owner Jonathon.

Janet sadly passed away in 2010 and in her memory we have decided to launch an annual innovation award.

Datesand would like to invite animal technicians and industry professionals to design a product that will then be marketed and sold within the biomedical science industry.

How it will work Anyone wishing to enter must complete this application pack and submit it with any supporting documents such as a description and drawing; to: jwiaward@datesand.com Once all the applications are in a team judges will look at all entries and select the 10 best designs that they feel will be best received by the industry and are the most viable.

These 10 will then be judged by and independent panel of industry professionals and Datesand management staff. From these 10 the judges will choose 3 finalists.

The final designs will be taken to the IAT Congress 2019 where visitors to the Datesand stand will be given the opportunity to vote for their favourite design.

The overall winner will be announced at the close of congress and the winner (if not there) will be notified.

Deadline for Entries

All entries must be sent to Datesand Ltd by 1st February 2019

Eligibility

Entrants must 18 years and over and work within the Bio Medical/Life Science industry.

Prizes

1st All expensive paid trip to any Life Science Trade Conference of your choice* 2nd 3rd

New iPad Pro ÂŁ200 Shopping Voucher

Page 1 4 | CamTechCare Newsletter | October 2018

How to Enter To enter the competition please ensure that you complete the following 5 steps.

1.

Read the terms and conditions

Page 6

2.

Complete the Entrant Details form

Page 3

3.

Complete the Design Specification including the full product description

Page 4 Page 5

4.

Print out and sign the terms and conditions

Page 6

5.

Submit the completed PDF, Signed T&C’s and any supporting documents such as drawings to Datesand Ltd Email: jwiaward@datesand.com Post: Datesand Ltd. PO Box 45, Manchester. M11 1XD

Page 2 CamTechCare Newsletter | October 2018 | 5

Entrant Details Name: Company/ Organisation:

Position:

Email:

Telephone:

Address: 35mm

70mm

My employment contract DOES NOT prevent me from entering the competition:

40mm

mm 35

60mm

134mm

Page 3 6 | CamTechCare Newsletter | October 2018

Design Specification Product Name: Type of Product: Short Description:

Materials Used: Manufacturing Process

3 R’s: Replacement

Reduction

Refine

Photo

Drawing

Sample

Animal Species:

How will the product benefit the animals:

What supporting info have you supplied:

Page 4 CamTechCare Newsletter | October 2018 | 7

Design Specification Detailed Description:

Page 5 8 | CamTechCare Newsletter | October 2018

T&C’s

(Terms and Conditions) Competition details form part of these terms and conditions.

Entry is open to anyone working in the Biomedical/Life Science industry except employees (and their families) of Datesand Ltd, its printers and agents, the suppliers of the prizes and any other companies associated with the competitions.

The entrant(s) must be aged 18 or over. Proof of identity and age may be required. Use of a false name or address will result in disqualification.

Company Contracts: If it is written into your employment contract that the copyright for anything you design belongs to the company, please do not submit an entry to this competition.

Entries that are incomplete, illegible or indecipherable will not be valid and will be deemed void.

All entries must be made directly by the person entering the competition.

Entries made online using methods generated by a script, macro or the use of automated devices will be void.

No responsibility can be accepted for entries lost, damaged or delayed in the post, or due to computer error in transit.

The prizes are as stated, are not transferable to another individual and no cash or other alternatives will be offered. The winner will be responsible for any income tax applied to the value of the prize and for informing the appropriate authorities.

The winner is responsible for expenses and arrangements not specifically included in the prizes, including any necessary travel documents, passports and visas.

The winner agrees to the use of their name, photograph and disclosure of country of residence and will co-operate with any other reasonable requests by Datesand Ltd. relating to any post-winning publicity.

Unless stated otherwise, all entries must be made on the official application form.

Closing date for entries is 1st Feb 2019 Reasonable efforts will be made to contact the winner(s). If the winner(s) cannot be contacted, or are unable to comply with these terms and conditions, the Promoter reserves the right to offer the prize to the runner(s)-up selected by the judges.

Confirmation of the prize will be made in writing to the winner(s).

Failure to respond or failure to meet the eligibility requirements may result in forfeiture of the prize.

The decision of the judges is final based on the criteria set out in the promotion and no correspondence will be entered into over this decision.

Competitions may be modified or withdrawn at any time. The provider of the prize is specified within the promotional material.

In the event of a discrepancy between these standard terms and conditions and the details in the promotional material (or any other terms and conditions provided/referred to at the time of entry), the details of the promotional material (and any other terms and conditions provided/referred to at the time of entry) will prevail.

weeks, unless otherwise stated, after the close of the competition. Only the surname and country of the winners will be disclosed.

The Prize The first prize is All expenses paid trip to any Life Science trade conference The product release date is the date that the product is launched using social media, advertising & marketing. This date will be at the discretion of Datesand Ltd. All design rights and itellectual property will be owned by Datesand Ltd BONUS PRIZE! Publication Award sponsored by Parsemus Foundation • The winner of the Janet Wood Innovation Award is eligible for a publication prize for research supporting the benefits of the product that is published in an open-access scientific journal.

• To encourage expeditious publication, an unrestricted publication bonus £1500 will be made available to the awardee immediately upon publication within one year of the award date.

• In addition to and separate from the unrestricted publication bonus directly to the awardee, publication fees will be covered if the researcher’s host institution does not typically cover researchers’ publication fees. Up to £2500 will be provided for publication fees in pure open-access journals such as PLoS or those that offer open-access. If the awardee’s institution covers publication fees, these funds will be made available directly to the awardee in addition to the £1500 publication bonus.

• In no instance will a publication award or publication fees be paid for: 1) a publication which will not be made freely available on PubMed immediately upon publication; or 2) publication in a predatory journal (as listed on Beall’s List of Predatory Journals or Publishers https://beallslist.weebly.com/). Publication of study results in such a journal will violate the terms of this agreement • In compliance with Research Information Network and Wellcome Trust guidelines for greater searchability, funding acknowledgements should include the exact phrase “This work was supported by Parsemus Foundation.”

Designs that are protected by copyright, patent or any other form of intellectual property will not be accepted into the competition.

Information that is supplied to Datesand Ltd will NOT Be held in confidence as it will be shown to the judging panel and if chosen as a finalist, will be displayed at the IAT Congress.

IMPORTANT! By submitting your competition entry, you are agreeing to these terms and conditions.

If you would like clarification on any of these terms and conditions please write to the promoter: Datesand Ltd. PO Box 45, Manchester. M11 3ER

A list of winners will be available by writing to the Promoter's address no earlier than six

I Agree to these Terms & Conditions.

Name: ................................................................. Signature: ............................................... Date: ...................................

Issue 3 Upadted: 16/05/2018

Page 6 CamTechCare Newsletter | October 2018 | 9

IAT Congress 2019 9th to 12th April 2019 Animal Technology: vital for global medical discovery The main purpose of Congress is to introduce Animal Technologists to the latest developments in all aspects of animal based research and technology. It is also an integral part of their CPD. Download the congress app from the Congress website: http://www.iat.org.uk/congress Registration Opens 10th September Congress 2019 will be based in a superb conference centre in North West England. The venue offers excellent flexible space for our scientific programme, trade exhibition and social events.

A full range of delegate packages will be available from "all inclusive" full attendance to single day attendance. Details and costings will be available shortly.

Registration details will be available here in the September. http://www.iat.org.uk/congress Scientific Programme Our varied Scientific Programme will offer a wide range of topics presented in Workshops, Paper Presentations and Posters.

Our CPD workshops are always popular with delegates and we again offer these on Tuesday afternoon and throughout the day on Wednesday and Thursday. Some workshops will be repeated to give as many delegates as possible the chance to attend.

Commercial Workshops We will again be hosting Commercial Workshops, slightly revamped from our initial concept in 2018. A workshop presentation area seating 30 delegates will be available within the exhibition area for exhibitors to present "hands-on" workshops on a topic of their choosing. The workshops will run for 30 minutes each and are free to attend. Delegates may book their attendance along with our CPD workshops online and on the Congress 2019 App.

10 | CamTechCare Newsletter | October 2018

CamTechCare Newsletter | October 2018 | 11

New monthly 3Rs podcast co-produced with LabAnimal https://soundcloud.com/labanimal-396053697/3min3rs0918 The NC3Rs have teamed up with LabAnimal, a Nature Research journal, to bring you “3 Minute 3Rs�, a new monthly podcast summarising the latest research and news in 3Rs science and technology.

This month, the podcast covers the following topics: Welcome to the September episode of 3-Minute 3Rs, brought to you

by Lab Animal (www.nature.com/laban), the NC3Rs (www.nc3rs.org. uk) & the North American 3Rs Collaborative (www.na3rsc.org) Here are the papers behind the pod: 1. Assessing animal affect: an automated and self-initiated judgment bias task based on natural investigative behaviour. doi.org/10.1038/s41598-018-30571-x 2. Comparison of serial blood collection by facial vein and retrobulbar methods in C57BL/6 mice. doi.org/10.30802/AALAS-JAALAS-17-000134 3. Can grimace scales estimate the pain status in horses and mice?

A statistical approach to identify a classifier. doi.org/10.1371/journal.pone.0200339 You can listen and subscribe to the podcast via SoundCloud or iTunes and let them know what you think via Twitter.

12 | CamTechCare Newsletter | October 2018

STOP!

Do you have a Personal Licence?

If not please contact UBS HO Licensing 01223 339900 or ubsHOLicencing@admin.cam.ac.uk

This is NOT a Personal Licence

This IS a Personal Licence

x√

These are the instructions on how to apply for and amend your Personal Licence

This is the certificate you receive after attending a Home Office Personal Licence course

Common Personal Licence problems • • • •

Thinking your training certificate is your Personal Licence Forgetting to amend your Personal Licence Doing procedures without a Personal Licence Forgetting to check Project Licence authority CamTechCare Newsletter | October 2018 | 13

Success at Bronze‌ Help us to Achieve Silver!

In June, Sarah, Lisa and Alan attended the Green Impact awards ceremony on behalf of the UBS Green Team to collect our Bronze Award. Our feedback from the auditor particularly praised the attempts to segregate recycling across all rooms.

Thank you so much to all of you who have helped UBS to achieve this award! It is down to the efforts of each and every one of you that we can make the Department a greener and more sustainable place to work.

We would particularly like to thank all of the members and contributors to the Green Team, so a big thank you goes out to: Polly Attlesey, Chris Brown, Gemini Chu, Aaron Dinsdale, Richard Drage, Emma Filby, Danielle Oberle, Sarah Stone, Colin Windle and Lisa Wright.

If you would like to know more about implementing green ideas in your facility feel free to contact the team directly or via your NACWO. There are some very simple ideas which can be quickly and easily implemented. Likewise we are always looking out for new ideas and new members. Contact us at: ubs-green-team@lists.cam.ac.uk

14 | CamTechCare Newsletter | October 2018

Representatives of the team will be attending the launch of the 2018-2019 scheme on Tuesday 16th October at 12pm in the Storey’s Field Centre. New attendees are very welcome! We are determined to achieve Silver next year so will report back on what we need to do to achieve that in our next report.

No doubt it will be a lot of hard work but hopefully a lot of fun too!

Please also remember to check in with the Environment and Energy websites which have ideas, resources and details of sustainability schemes including discounts and offers: https://www.environment.admin.cam.ac.uk/

14th Congress 2019 FELASA, in association with CLASA Prague, Czech Republic - 10-13 June 2019

Watch out for the opening of the abstract submission!

The FELASA 2019 scientific programme is already well advanced and many topics are addressed: • Animal Research Reporting: aid to experimental design, responsible research, funding and reporting 3R research • Institutional challenges: communication, transparency, facility management • Ethical challenges: AWB abilities, ending severe suffering, pain assessment • Education and training: accreditation, animal carer education, competency assessment, managers' education • Severity classification workshops: 1 train the trainers, 4 severity and classification • European Directive revision and remaining implementation challenges • Rodent health monitoring, microbiota, gnotobiology, thermoneutrality • FELASA Working Groups incl. farm animals, cephalopods and transport • Aquatics health monitoring, severity classification and euthanasia http://www.felasa.eu/announcements/felasa-14th-congress-2019 http://www.felasa2019.eu/

CamTechCare Newsletter | October 2018 | 15

Mouse handling webinar https://www.nc3rs.org.uk/mouse-handling-webinar In this webinar, which originally took place on 27 April 2018 at 11am (BST), Professor Jane Hurst of the University of Liverpool shares the evidence base supporting refined mouse handling techniques as well as practical advice and tips for implementing them in your institution.

16 | CamTechCare Newsletter | October 2018

3Rs Relevant Publications Exploring the Role of Animal Technologists in Implementing the 3Rs: An Ethnographic Investigation of the UK University Sector.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027776/ Greenhough B1, Roe E2.

Abstract

The biomedical industry relies on the skills of animal technologists (ATs) to put laboratory animal welfare into practice. This is the first study to explore how this is achieved in relation to their participation in implementing refinement and reduction, two of the three key guiding ethical principles-the “3Rs”--of what is deemed to be humane animal experimentation. The interpretative approach contributes to emerging work within the social sciences and humanities exploring care and ethics in practice. Based on qualitative analysis of participant observation within animal research facilities in UK universities, in-depth interviews with ATs, facility managers, and other stakeholders, and analysis of regulatory guidelines, we draw a contrast between the minimum required of ATs by law and how their care work not only meets but often exceeds these requirements. We outline how ATs constitute a key source of innovation and insight into the refinement of animal care and the reduction of animal use, hitherto not formally acknowledged. Exploring AT care work as an example of ethics in practice makes an original contribution to broader debates within health care and animal welfare about how technology, regulation, and behavior can foster and sustain a “culture of care”.

Effects of light at night on laboratory animals and research outcomes. Behav Neurosci. 2018 Aug;132(4):302-314. doi: 10.1037/bne0000252. Epub 2018 Jun 28.

Emmer KM1, Russart KLG1, Walker WH1, Nelson RJ2, DeVries AC3.

Abstract

Light has substantial influences on the physiology and behavior of most laboratory animals. As such, lighting conditions within animal rooms are potentially significant and often underappreciated variables within experiments. Disruption of the light/dark cycle, primarily by exposing animals to light at night (LAN), disturbs biological rhythms and has widespread physiological consequences because of mechanisms such as melatonin suppression, sympathetic stimulation, and altered circadian clock gene expression. Thus, attention to the lighting environment of laboratory animals and maintaining consistency of a light/dark cycle is imperative for study reproducibility. Light intensity, as well as wavelength, photoperiod, and timing, are all important variables. Although modern rodent facilities are designed to facilitate appropriate light cycling, there are simple ways to modify rooms to prevent extraneous light exposure during the dark period. Attention to lighting conditions of

laboratory animals by both researchers and research care staff ensures best practices for maintaining animal welfare, as well as reproducibility of research results.

Partial cage division significantly reduces aggressive behavior in male laboratory mice https://doi.org/10.1177/0023677217753464 Bret R Tallent, L Matthew Law, Rachel K Rowe Abstract

Aggression in mice often results in injury leading to unplanned euthanasia or the initiation of protocols to isolate animals, thereby increasing research costs and straining resources. Here, we tested if adding a partial cage divider into existing mouse cages affected aggressive-like behavior in group-housed male mice (18 mice; 3 per cage). Mice were randomly assigned to one of two groups upon arrival to the vivarium: (1) standard cage; (2) cage with a partial cage divider. Behavioral observation over 12 hours were conducted at day one, two, and seven after receipt at the facility in order to assess aggression during the course of establishing dominance hierarchies. Observers blinded to study design and hypothesis scored each video for the number and type of aggressive behaviors, which were summed for each hour and analyzed. Results indicated a statistically significant decrease in aggressive behaviors of mice in cages with dividers compared to mice in standard cages. We conclude that cage dividers, which resemble burrows and provide access to common food/water, may promote rigorous research by reducing the number of animals used in a study and refining housing, thus, improving animal welfare.

FELASA accreditation of education and training courses in laboratory animal science according to the Directive 2010/63/EU. Lab Anim. 2018 Jul 24:23677218788105. doi: 10.1177/0023677218788105. [Epub ahead of print] Gyger M1, Berdoy M2, Dontas I3, Kolf-Clauw M⁴, Santos AI⁵, Sjöquist M⁶.

Abstract

This document describes how the Federation of European Laboratory Animal Science Associations (FELASA) accreditation addresses both the Directive 2010/63/EU and the related European Commission guidance document. The four EU Functions and beyond: FELASA accredits courses that fulfil the requirements of Functions A, B, C and D as defined by EU Directive, Article 23, as well as for designated veterinarians and specialists in laboratory animal science. Modularity and mobility: Cohesive courses for Functions and for very specific topics are accredited, but flexibility and mobility are possible: a researcher can start his/her training with one FELASA accredited course and complete other modules with another. A course organizer will deliver a FELASA certificate relating to the successfully completed modules. Accreditation process: The process consists

CamTechCare Newsletter | October 2018 | 17

of two major steps: (1) a review of full course documentation provided by the applicant will lead, if successful, to FELASA accreditation. The course is posted on the FELASA website as ‘FELASA accredited’ and the course provider can deliver FELASA certificates upon successful completion of the course; (2) successful accreditation is followed by an on-site course audit. In the case of a negative outcome of the audit, FELASA accreditation is withdrawn, the course is deleted from the list of FELASA accredited courses and FELASA certificates cannot be issued. To ensure that quality is maintained, continuation of accreditation requires regular revalidation.

Responsibility and Laboratory Animal Research Governance.

Sci Technol Human Values. 2018 Jul;43(4):723-741. doi: 10.1177/0162243917727866. Epub 2017 Sep 1.

McLeod C1,2, Hartley S3.

Abstract

The use of animals in experiments and research remains highly contentious. Laboratory animal research governance provides guidance and regulatory frameworks to oversee the use and welfare of laboratory animals and relies heavily on the replacement, reduction, and refinement (3Rs) principles to demonstrate responsibility. However, the application of the 3Rs is criticized for being too narrow in focus and closing down societal concerns and political questions about the purpose of animal laboratory research. These critiques challenge the legitimacy of responsibility in laboratory animal research governance and call for new approaches. With the advent of the “Responsible Research and Innovation” (RRI) agenda, we investigate whether the notion of responsibility in the controversial area of animal research governance could be enhanced by examining the 3Rs through RRI. Our analysis reveals RRI has the potential to helpfully augment the 3Rs in three key ways: recognizing the need to include a broader range of experts and publics in animal research governance; emphasizing the importance for animal research scientists of taking societal, and not just role, responsibilities into account; and acknowledging the political questions animal research raises.

From 3Rs to 3D: In vitro alternative models for replacement.

Meeting Report https://www.altex.org/index.php/altex/ article/view/1034/1136 Nagy AL1, Catoi C1, Socaciu C1, Pintea A1, Oros NA1, Coman C1, Rugina D1, Matea CT2,3, Mocan T2,3, Coccini T⁴, De Simone U⁴, De Angelis I⁵, Bertero A⁶, Sambuy Y⁷, Caloni F⁶.

Abstract

On February 21, 2018, a third meeting was held at the University of Agricultural Sciences and Veterinary Medicine (USAMV) in Cluj-Napoca, Romania with representatives of the Romanian Center for Alternative Test Methods (ROCAM), the Italian Platform on Alternative Methods (IPAM) and the Italian

18 | CamTechCare Newsletter | October 2018

Association of in vitro Toxicology (CELLTOX). The main objective of the meeting was to promote alternative testing models in research and education and to present different in vitro models to be used for xenobiotic testing.

Environmental enrichment prevents pup mortality in laboratory mice.

Lab Anim. 2018 Jan 1:23677218777536. doi: 10.1177/0023677218777536. [Epub ahead of print] Leidinger CS1, Thöne-Reineke C2, Baumgart N1, Baumgart J1.

Abstract

Early death of mouse pups is a commonly known problem in breeding mice colonies, which is still often regarded as ‘normal’ or is even overlooked due to the counting procedures applied.

As reduced breeding performance probably indicates reduced well-being, this seems to be an underestimated welfare issue in laboratory mouse breeding. The present study compares the influence of three different forms of enrichment in breeding cages on infant survival rate and development of C57BL/6J mice. Our data reveal that lack of enrichment results in greater preweaning pup mortality, reduced weight and delayed development. Changing the environmental conditions after birth cannot prevent litter loss but improves the development of pups born in impoverished environments. Overall, our results underline the importance of early counting of mice for optimizing refinement strategies to ensure well-being and breeding success.

Promoting 3Rs in Finland

Meeting Report https://www.altex.org/index.php/altex/ article/view/1130/1137 Vuorenpää, H. 2018. Promoting 3Rs in Finland. ALTEX - Alternatives to animal experimentation. 35, 3 (Jul. 2018), 425. DOI:https://doi.org/10.14573/altex.1807021.

Abstract The Finnish National Consensus Platform for Alternatives (Fincopa) organized its annual seminar in Helsinki on May 21, 2018 at the Finnish Ministry of the Interior. This year the seminar focused on current activities in promoting the 3Rs in Finland with four invited speakers. The seminar venue was provided by the Finnish Ministry of Agriculture and Forestry with the possibility for remote attendance via Skype.

Aspects of Good Colony Management Workshop 24th October UBS, Greenwich House, Madingley Road

All Animal Techs and Named Persons Welcome!

Image curtesy of

Please come prepared with discussion ideas 9.30 Introduction - Colony Manager 10.00 MCMS database - Maryann Haskings- UBS 10.30 Lifecycle of a CRISPR colony Hannah Ward-Jones, Sanger Primary colony manager 11.00 Break 11.30 Animal Welfare and Health Aurelie Thomas, Sanger NVS and Amelia Phillips, UBS NVS

University Biomedical Services

12.00 What Works, What Doesn’t- Group discussion Outcome-Working Group 12.30 Lunch and Networking

CamTechCare Newsletter | October 2018 | 19

Events Diary October 15-16

ESLAV-ECLAM-AAALAC SECAL Conference, Barcelona http://www.barcelonacongress2018.com/modules. php?name=webstructure&idwebstructure=16

October 28

AALAS Baltimore, Maryland, USA https://www.aalas.org/national-meeting

October 30

RSPCA/UFAW Rodent and Rabbit Welfare meeting, London here.

November 5-6

Danish 3Rs Symposium, Copenhagen https://en.3rcenter.dk/symposium/symposium-2018/

November 15

NC3Rs Primate Welfare meeting, London https://www.nc3rs.org.uk/events/2018-primate-welfaremeeting

November 20 and 21

SGV meeting, CHUV, Lausanne, https://naturalsciences.ch/organisations/sgv/meetings

November 27-29

LASA Annual Conference 2018, Birmingham LASA website email info@lasa.co.uk.

December (date unknown)

RSPCA Lay Members’ Forum, London https://www.rspca.org.uk/home

April 9-11

IAT Congress 2019 http://www.iat.org.uk/congress

June 10-13

FELASA Congress 2019, Prague http://www.felasa.eu/announcements/felasa-14thcongress-2019

IAT Events Calendar http://www.iat.org.uk/calendar NC3Rs Events Calendar www.nc3rs.org.uk/event.asp?id=33 FGB Events Calendar https://www.fondazioneguidobernardini.org/en/ training_initiatives/events.aspx?IDEventType=3 AALAS Events Calendar https://www.aalas.org/calendar

20 | CamTechCare Newsletter | October 2018

University of Cambridge News Feeds

Scientists develop mouse ‘embryo-like structures’ with organisation along body’s major axes

Protein discovery may explain why some patients develop resistance to new class of anticancer drugs

Scientists generate key life event in artificial mouse ‘embryo’ created from stem cells

03 Oct 2018

25 Jul 2018

23 Jul 2018

A team of scientists at the University of Cambridge has developed an artificial mouse embryo-like structure capable of forming the three major axes of the body. The technique, reported today in the journal Nature, could reduce the use of mammalian embryos in research.

A team of researchers at the University of Cambridge has identified a protein complex that might explain why some cancer patients treated with the revolutionary new anti-cancer drugs known as PARP inhibitors develop resistance to their medication.

The creation of artificial embryos has moved a step forward after an international team of researchers used mouse stem cells to produce artificial embryo-like structures capable of ‘gastrulation’, a key step in the life of any embryo.

Read More

Read More

Read More

RSPCA/UFAW Rodent and Rabbit Welfare meeting, The 25th edition of our popular rodent and rabbit welfare meetings will be held in central London on 30 October 2018.

Presentations will cover refinements in housing; nest building behaviour in rats; welfare implications of identification methods in mice; classifying the severity of repeated anaesthesia; and the contribution of modern imaging techniques to the 3Rs. There will also be an update on relevant issues from the Home Office Animals in Science Regulation Unit.

You can register to attend this event here.

CamTechCare Newsletter | October 2018 | 21

Funding Opportunities Laboratory Animals Limited provides funding for a range of

education and training initiatives in the field of laboratory animal science.

Deadlines for applications are 31st of March and 30th of September annually Individual scientists, who wish to attend training (courses) in laboratory animal science and welfare, can apply for bursaries from Laboratory Animals Limited. In the application a clear and concise statement outlining why attending the course is necessary and relevant for the work of the applicant should be provided. http://www.lal.org.uk/funding-and-opportunities

Lasa Bursaries

http://www.lasa.co.uk/bursaries/ LASA bursaries can be applied for at any time of the year and for any event considered relevant to LASA’s mission statement.

Animal technicians, post-graduate students and others for whom funds may be limited are especially encouraged to apply.

Download bursary rules and form.

Universities Federation for Animal Welfare

https://www.ufaw.org.uk/grants-and-awards/ufaw-grants-and-awards Small Projects and Travel Awards Awards up to ÂŁ3,500 for research and other projects, and awards to support travel.

Research and Project Awards Awards over ÂŁ3,500 for research or other animal welfare projects.

22 | CamTechCare Newsletter | October 2018

New platform to maximise the 3Rs impact of NC3Rs-funded research The NC3Rs Gateway is a new publication portal on the F1000Research publication platform dedicated to the 3Rs, featuring methodology articles, reviews and source data that describe in detail how the use of animals in research can be replaced, reduced or refined. All articles will be published almost immediately and then undergo invited open peer review

CONGRESS Invitation to Participate

Congress 2019 9th – 12th April 2019

CALL FOR POSTERS l take an active part in the leading annual meeting for animal

technologists

2019

l submit a poster and receive a discount – final date Thursday

28th February 2019* l choose to do an oral presentation of your poster and receive

a further discount – closing date Wednesday 19th December 2018* l send your ideas today on the Submission form available from

www.iat.org.uk (*posters will not be accepted for display at Congress unless they have been properly submitted and approved by the Congress Committee) l prize for the best poster – plus claim up to 10 CPD

points – details given on acceptance of submission

Contact: congress@iat.org.uk

Animal Technology – vital for global medical discovery CamTechCare Newsletter | October 2018 | 23

CONGRESS Invitation to Participate

Congress 2019 9th – 12th April 2019

CALL FOR WORKSHOPS l take an active part in the leading annual meeting for animal

technologists

2019

l do you have an area of expertise? (i.e. work with a more

unusual species, bio-security, management, health & safety, been involved in a new build, environmental enrichment, GA breeding, ageing animals, transport, etc) l could you run a 1 - 3 hour interactive workshop and

qualify for a free congress? l send your ideas today on the Submission form available from

www.iat.org.uk l final date for submissions: Friday 16th November 2018

Contact: congress@iat.org.uk

Animal Technology – vital for global medical discovery

CONGRESS Invitation to Participate

Congress 2019 9th – 12th April 2019

CALL FOR PAPERS l take an active part in the leading annual meeting for animal

technologists

2019

l present a paper and qualify for free attendance at Congress l make this your debut presentation year – first time presenter

papers are only 20 minutes long and as well as a free congress there is a prize for the one judged to be the best l send your ideas today on the Submission form available from

www.iat.org.uk l final date for submissions: Friday 16th November 2018

Contact: congress@iat.org.uk

Animal Technology – vital for global medical discovery 24 | CamTechCare Newsletter | October 2018

AS-ET Annual Lecture London

Two for the price of one: twinning – the ultimate regeneration?

Professor Claudio Stern

BSc, PhD, DSc, FRSB, FRMedSci, FRS J Z Young Professor of Anatomy University College, London

____________________________________________________________ 5.00pm Wednesday 17th October 2018 King’s College, London The lecture will be followed by a wine reception

________________ Admission Free

Order Tickets from http://www.animalsinscienceeducationtrust.org.uk/annuallecture Sponsored by

CamTechCare Newsletter | October 2018 | 25

AS-ET Annual Lecture Manchester

Harnessing Stem Cells for Regenerative Medicine: how far have we got?

Professor Sir Richard Gardner

MA, PhD, ScD, FRSB, FIAT (Hon), FRS

Emeritus Research Professor, Royal Society Emeritus Student, Christ Church College, Oxford ____________________________________________________________ 5.00pm Wednesday 7th November 2018 Manchester University The lecture will be followed by a wine reception

________________ Admission Free

Order Tickets from http://www.animalsinscienceeducationtrust.org.uk/annuallecture Sponsored by

26 | CamTechCare Newsletter | October 2018

Mirror, Mirror, on the Wall Pauline Reading, Reece Reading and Callum Branstone

The Problem Single housed male mice are a large problem in all facilities because as an industry we want to move away from keeping mice alone.

Mice are a social species and as such we should be taking every opportunity we can to group house them but when males keep fighting each other that’s easier said then done.

The mirror method hopes to encourage single housed male mice to be grouped with others without any fighting.

The Perception When mice are grouped together for the first time it is a novel experience filled with new sights and smells which will stress the animal out and may cause aggression.

It has been proven that mice can see a reflection of another mouse in the mirror so by adding a mirror prior to grouping they become accustomed to the sight of another mouse.

Scents cling to bedding and nesting material so swapping over both between mice being grouped also allows for them to be habituated to each other’s smells.

By the time the mice are introduced they should be used to the sights and smells of another animal reducing stress and the likelihood of fighting.

The method

Step 1

Step 2

• Two single housed males of a similar age are picked.

• A mirror is placed inside each cage hanging from the grid using a hook. It should be in a central position.

Number of successful pairings between mice with and without mirrors 20

Step 3

Step 4

• A handful of bedding and nesting material should be swapped over daily and placed by the mirror.

This should be repeated for 2-3 days.

• On the final day the mice should be placed together in a clean cage with no mirror. Their cage should be left off of the rack and observed regularly on the first day.

Number of successful pairings between mice of different age groups 8

Result • The mice should now be living with each other coexisting without any signs of aggression.

Hints and tips Though this method is successful it is not guaranteed.

Mice that do no pair together will fight.

18

7

Observation skills are critical during the start of their pairing.

16 6

Any signs of fighting should lead to mice being split.

5

This method can be retried on them at a later date.

14 12 10

The cage should not be cleaned during the first week.

4

8

3

6 2 4 1

2

0

0

Mice with a mirror

Success Failure

Mice without a mirror

Mice under 6 months old

Mice 6-9 months old Mice 9-12 months old Success Failure

Mice over 12 months old

Summary This method currently has a 78% success rate and has been used across a variety of different strains including different strain pairing.

The mirror has been proven to be a vital part of the strategy as only 16% of non-mirror pairings were successful.

It is also shown that the age of the mice does not make a difference so this method can be used on virtually any single mouse.

Using this method can refine animal research allowing more animals to be grouped and reduce the number of mice that are culled for being single housed.

More work will be done to refine this method but if adopted it may help make a reduction in this industries single housed male problem.

PMR17@LE.AC.UK, RR197@LE.AC.UK, CLB72@LE.AC.UK

CamTechCare Newsletter | October 2018 | 27

A summary of literature on mouse aggression on YouTube

by Brianna Gaskill Some of you will be aware that the NC3Rs is leading a data crowdsourcing project for animal technicians to collect data on the prevalence and potential triggers of cage aggression in group-housed male mice. https://www.nc3rs.org.uk/laboratory-mouse-aggression-study With this in mind Mark Prescott (NC3Rs) recently mentioned this in his talk at a LAVA conference our NVS attended and detailed two presentations worthy of watching:

https://www.youtube.com/watch?v=pmp4QWWeZLs

https://www.youtube.com/watch?v=ayDe032MiIQ 28 | CamTechCare Newsletter | October 2018

The HUB The ASC newsletter for AWERBs

Issue 3, October 2018

Your ASC AWERB Subgroup

Sally Robinson (Chair)

John Landers

Clare Stanford

Donald Bruce

Wendy Jarrett

Gail Davies

Ken Applebee

Dear colleague,

2018 ASC Road Shows

Welcome to the third AWERB Hubs network newsletter from the Animals in Science Committee (ASC). In this edition we provide additional links to useful resources and materials and introduce the AWERB Knowledge Hub Group, an interactive online communication platform designed specifically for AWERBs.

The ASC is excited to announce that it will be hosting two AWERB Road Shows this autumn. The first will be hosted at University of Birmingham on the 11th October 2018, and the second at Newcastle University on the 7th November. The road shows will cover a variety of subject matters, including tips on improving non-technical summaries, how AWERBs incorporate ethics, introducing the launch of the AWERB ‘Knowledge Hub’ (for more details on this, see page 2). The road show will also address an AWERB’s role in ensuring that methodology implements refinement to the full, as well as advice to AWERBs on how to prevent breeding of surplus animals.

The ASC wants to hear from you!

You may not be aware that the work of the ASC is varied and dynamic. Did you know that the ASC responded to the previous Research Excellence Framework Consultation resulting in the establishment of animal research as a topic of assessment where it was not being considered? The ASC endeavours to promote the importance of the welfare of animal used in research and its ethics.

We would like to invite AWERBs to consider how you as a member of the animal welfare ethical review community, can better inform the ASC so that we can do more for you? Please send your suggestions to the ASC secretariat: (asc.secretariat@homeoffice.gsi.gov.uk).

Your responses will feed into the ASC work programme and may be used in a forthcoming presentation at this year’s annual LASA conference.

Recent tips for writing Non-technical Summaries The latest guide from UAR for writing Non-technical summaries is now available – see ‘Useful links’. The guide was created to help applicants with writing NTSs. The guide explains the purpose of the NTS and the common mistakes when writing one.

Gilly Stoddart

Useful links Right click & open

RSPCA-LASA Guiding principals on good practice

2018 Hub Workshop Report

LASA Annual Conference

Guide to writing Nontechnical Summaries

C Crown Copyright 2018 CamTechCare Newsletter | October 2018 | 29

The HUB The ASC newsletter for AWERBs

Issue 3, October 2018

Introducing the AWERB Knowledge Hub Group At the AWERB 2018 ASC and AWERB Hub chairs workshop, it was made clear that there is a need for AWERBs to be able to share information and communicate with each other. Due to the need to maintain the privacy of AWERBs across the UK, this has been difficult to facilitate.

A great idea was suggested, which was to have an online platform for all AWERBs where they would be able to discuss their ways of working, ask questions and put forward ideas.

NCR3s Events

The group offers a variety of facilities, available to all AWERBs, these include:

As a result, a virtual community has been created, which allows all AWERB members from across the UK to ‘talk’ to 1. An online Forum, where members can ask questions, discuss ideas, each other and enables them to share share learning and provide ideas, collaborate on work and promote solutions. the diffusion of best practice for use of animals in science.

2. A Library, where AWERBs can upload Topics currently up for discussion on the and share documents to bring AWERB Knowledge Hub Group include, expert guidance and resources but are not limited to: working with together. animals abroad, implementing the 3R’s, ethics and the function of AWERBs. 3. An Events page where members can promote forthcoming events.

If you are an AWERB member and interested in becoming a member of the 4. A Wiki page for AWERBs to share AWERB Knowledge Hub Group, please knowledge and collaborate on wiki contact the ASC Secretariat on (editable web) pages. (asc.secretariat@homeoffice.gsi.gov.uk).

The group is set to be officially launched 5. An Ideas page, where members can in the autumn. share ideas with the group.

Members may vote for their favourite ideas.

C Crown Copyright 2018 30 | CamTechCare Newsletter | October 2018

Useful links

Right click & open

EU Guidance Documents

RSPCA Events

AWERB network leaflet

The HUB The ASC newsletter for AWERBs

Recent ASC Publications A number of publications have been published since the last Hub Newsletter in April 2018, including: 1. Summaries of the work streams to be taken forward by the ASC task and finish groups between 2018-2019.

2. The AWERB Hub Network Leaflet.

3. The fourth ASC and AWERB Hubs workshop report was published, summarising the discussions and themes of the workshop.

4. Following on from the Harm Benefit Analysis review, the key recommendations to be referred to ASRU were published

Issue 3, October 2018

Events AWERB Road Show - Birmingham The ASC will be holding an AWERB Road Show on the 11th October at the University of Birmingham. All AWERB members are invited to attend this event, which will promote useful discussion on ethics, NTS, dealing with surplus animals and more.

RSPCA/UFAW 25th Rodent and Rabbit Welfare Meeting This year's RSPCA/UFAW Rodent and Rabbit Welfare Meeting will be held in central London on 30th October. Please note that the closing date for registration is Monday 10th October 2018.

AWERB Road Show – Newcastle The ASC will be holding an AWERB Road Show on the 7th November at the University of Newcastle. All AWERB members are invited to attend this event, which will promote useful discussion on ethics, NTS, dealing with surplus animals and more.

Useful links

Right click & open

RSPCA-LASA Guidance on developing introduction material for AWERBs

RSPCA-LASA-LAVAIAT Guidance on delivering effective ethical review

Implementation of the Directive 2010/63/EU (2014)

NC3Rs - New checklists for consideration of work using living animals conducted overseas

C Crown Copyright 2018 CamTechCare Newsletter | October 2018 | 31

CamTechCare

Keeping you informed

Contact Information UBS Enquiries: ubsenquiries@admin.cam.ac.uk UBS Training enquiries: ubssts@admin.cam.ac.uk UBS Website: www.ubs.admin.cam.ac.uk/user UBS Home Office Licensing: ubsHOLicencing@admin.cam.ac.uk