Zeigler - Lab Catalog by Zeigler Bros., Inc.

Research Diets | Research Services

Health & Research Products Catalog

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Table of Contents Notes:

Table of Contents Introduction 2 Mission 3 N u t r i t i o n an d F o r m u l at i o n 4 L ab o r at o r y A n i m al D i et S u m m ar y

2 3 4

Rodent Diets 5 6 7 8 9 10 11 12 13 14 15 16

General Information Rodent RQ 18-6 Rodent RQ 18-9 Rodent RQ 19-11 Rodent RQ 22-5 Rodent RQ 22-9 Rodent NIH-07 Open Formula Rodent NIH-31 Open Formula Auto Rodent NIH-31 Modified Auto Phytoestrogen Reduced I 22.5-5 Phytoestrogen Reduced II 18-5 Rodent NTP-2000

5 6 7 8 9 10 11 12 13 14 15 16

Rabbit Diets 17 18 19 20 21

General Information Rabbit RQ 16-3 Rabbit RQ Hi-Fiber 14-2.5 Rabbit NIH-09 Open Formula Rabbit NIH-32 Open Formula Auto

17 18 19 20 21

Guinea Pigs 22 General Information 23 Guinea Pig RQ 18-4 24 Guinea Pig NIH-34M

22 23 24

Large Animal Model Diets 25 General Information 26 Swine NIH-2004-2 27 Ungulate NIH-11

25 26 27

Aquatic Diets 28 29 30 31

General Information Adult Zebrafish Diet Adult Xenopus Diet Aquatox Flake

28 29 30 31

Research Diets 32 33 34 35 36

General Information Purified Rodent Diet AIN-76A Purified Rodent Diet AIN-93G Purified Rodent Diet AIN-93M Stroke Prone Rodent Diet

32 33 34 35 36

Additional Information 37 Diet Certification Program 38 Services and Capabilities 39 Ordering Information 06/11

37 38 39 06/11

Mission

“Building value to life through innovative nutrition”

We strive to build our business around a strategy of innovative nutrition. Foods that provide for proper nutrition help to bring value to life for both man and animals. Through our involvement in the biomedical community, we work to develop diets aimed at assisting in the resolution of human illness and disease. Our pet and zoo line of products are directed toward improving nutrition for some of the world’s most precious wildlife, and the diets we develop for use in aquaculture help to provide an assortment of healthy, nutritious seafood for human consumption. Our team is committed to serving your nutritional needs. Quality is of utmost importance to us, whether it be through the value of a product or the integrity of a relationship. We understand that our success depends, not only on the performance of our products, but more importantly, on the strength of relationships established with our customers and suppliers. We look forward to being part of your success and welcome all opportunities to improve.

Sincerely,

Thomas R. Zeigler, Ph.D. Chairman of the Board

Zeigler Bros., Inc. 400 Gardners Station Road Gardners, PA 17324 USA 717-677-6181 phone www.zeiglerfeed.com

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Nutrition and Formulation

FixedFormulations Know Whatâ&#x20AC;&#x2122;s in your Diet!

It is recognized that nutrition is the most important environmental factor influencing the ability of laboratory animals to reach their genetic potential for growth, reproduction, longevity, and to respond to environmental stress. Therefore, it is essential for research scientists, veterinarians, and animal facility managers to have detailed dietary information in order to make valid conclusions regarding experimental results. Zeigler provides lab diets which are comprised of fixed formulations, where ingredient compositions remain constant and are not altered to accommodate the use of lower quality or less costly raw materials. Fixed formulations help decrease the risk of non-assayed or undesirable constituents which could influence

experimental results or jeopardize the well being of animals. Nutrient concentrations in Zeigler lab diets are controlled by procurement of ingredients that contain acceptable specified nutrient concentrations and meet Zeiglerâ&#x20AC;&#x2122;s specified quality standards. Zeigler supports the fixed formula diet concept because it is important for scientists to know and have control of the nutrient and contaminant concentrations of diets used in biomedical research. In addition to specified nutrient and contaminant assays, the researcher is offered the option of complete formula disclosure to help monitor and control ingredient inputs on every lot of diet. With this information, the scientific community can have the highest level of confidence in producing sound experimental results.

Laboratory Animal Diet Summary

Product Code

Product Name

Rodent Diets 411005 411003 411004 411000 413000 410710 413110 413120 41200 541201 420000 Rabbit Diets 421800 422100 410900 413200 Guinea Pig Diets 413600 413400

Crude Protein (Min%)

Crude Fat (Min%)

Crude Fiber (Max%)

Total Ash (Max%)

Rodent RQ 18-6 Rodent RQ 18-9 Rodent RQ 19-11 Rodent RQ 22-5 Rodent RQ 22-9 Rodent NIH-07 Open Formula Rodent NIH-31 Open formula Auto Rodent NIH-31 Modified Rodent Phytoestrogen Reduced I Rodent Phytoestrogen Reduced II Rodent NTP-2000

18.0 18.0 19.0 22.0 22.0 22.5 18.0 18.0 18.0 22.5 13.0

6.0 9.0 11.0 5.0 9.0 5.0 4.0 5.0 5.0 5.0 8.0

4.0 4.0 4.0 4.0 4.0 4.0 5.0 5.0 4.5 3.5 12.0

8.0 8.0 8.0 7.0 7.0 7.5 8.0 8.0 7.5 7.5 8.0

Rabbit RQ 16-3 Rabbit RQ Hi-Fiber 14-2.5 Rabbit NIH-09 Open Formula Rabbit NIH-32 Open Formula Auto

16.0 14.0 16.5 16.0

3.0 2.5 2.4 2.5

15.0 22.0 16.8 15.8

8.0 10.0 10.0 8.0

Guinea Pig RQ 18-4 Guinea Pig NIH-34 M

18.0 17

4.0 3.4

14.5 13.5

8.0 8.5

Large Animal Model Diets 415675 Swine NIH-2004-2 415571 Ungulate NIH-11

14.0 14.0

4.0 3.5

5.0 12.5

8.0 10.0

Aquatic Diets 388763 316518 545000

Adult Zebrafish Diet Adult Xenopus Diet Aquatox Flake

55.0 40.0 43.0

15.0 10.0 13.0

1.5 3.5 1.5

8.0 7.0 10.5

Research Diets 527500 510000 520000 528800

AIN-76A AIN-93G AIN-93M Stroke Prone Rodent Diet

16.0 17.9 12.6 16.0

5.0 7.0 4.0 3.8

5.0 4.8 4.8 2.0

4.0 4.2 3.9 5.5

Certified Diets All Zeigler laboratory animal diets can be certified to accommodate the requirements of specific research protocols. Irradiated Diets Most Zeigler laboratory animal diets can be purchased as an irradiated product. Research Diets Zeigler offers an extensive range of custom research diets. Please contact us for more information.

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Rodent Diets

General Information

Zeigler provides a series of rabbit diets with differing ingredient and nutrient concentrations to accommodate colonies maintained for the production and use in biomedical research, or other commercial endeavors. Diets also differ in nutrient composition to accommodate rabbits of different genotypes, and for differences in requirements during the life cycle: growth, reproduction, and maintenance.

Rodent Research Quality (RQ) Diets Zeigler Rodent RQ Diets are ﬁxed formula diets that provide those nutrients required for maximum reproductive performance in rat and mouse production and research colonies maintained in conventional environments. Zeigler Rodent RQ diets are “Closed Formula” or proprietary formula diets in that the quantitative ingredient concentrations are not readily available.

The National Institutes of Health (NIH) Open Formula Diets Zeigler Rodent NIH Open Formula Diets were developed at NIH as standard reference diets for use throughout the biomedical research community. These diets are referred to as “Open Formula” diets because the quantitative ingredient composition is readily available. Zeigler was the ﬁrst company to make the NIH diets commercially available approximately 35 years ago. Zeigler also manufactures and markets the National Toxicology Program 2000 Diet (NTP-2000), an open formula diet speciﬁcally formulated for the bioassay studies conducted for the NTP.

Diets Limited in Phytoestrogen Concentrations The dietary concentrations of phytoestrogens are a concern in various toxicological and biomedical research programs. To accommodate the requirements of these programs, Zeigler nutritionists formulated diets with lower phytoestrogen concentrations. Zeigler modiﬁed the NIH diets to minimize the naturally occurring phytoestrogen contributions of soybean meal, soybean oil and alfalfa meal. The resulting formulations are offered to you as the Rodent Phytoestrogen Reduced diets.

Utilization of Rodent Diets Shelf Life:

Zeigler’s all natural ingredient rodent diets are formulated for a shelf life of six months after the date of manufacture, which is consistent with the recommendations in the NIH “Guide for the Care and Use of Laboratory Animals.” Laboratory animal diets should be stored in cool, dry areas where the humidity is low in order to realize maximum shelf life. In addition, feed storage areas should be free of vermin and hazardous materials in order to prevent biological or chemical contaminations.

Sizes:

Zeigler rodent diets are most frequently manufactured in binamic 8 pellets (5/8”-3/4” wide, 3/8”- 7/16” thick, 3/8”1” long). These diets can also be manufactured into pellets of various other sizes, or in meal form, to accommodate speciﬁc customer requirements. Utilization of Rodent Diets

Packaging:

Zeigler rodent diets are packaged in 3-ply laminated paper bags of varying weights to accommodate customer requirements.

Feeding Zeigler rodent diets are formulated for ad libitum feeding. Adult rats will consume 15-20 grams of feed per day, while Instructions: adult mice will consume 4-6 grams of feed per day. Clean, fresh water should be available ad libitum.

08/10

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Rodent RQ 18-6 for Rats and Mice Colonies The Rodent RQ 18-6 (18% protein, 6% fat) diet is formulated to provide the nutrients required for maximum reproductive performance by rat and mice colonies maintained in conventional environments.

Ingredients

Wheat, Corn, Wheat Middlings, Fish Meal, Soybean Meal, Soy Oil, Alfalfa Meal, Corn Gluten Meal, Brewers Dried Yeast, Dicalcium Phosphate, Limestone, Salt, Choline Chloride, L Lysine, DL Methionine, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E), Folic Acid, Niacin, Riboﬂavin, Thiamine Mononitrate, Vitamin B12 Supplement, Pyridoxine Hydrochloride, Biotin, Vitamin A Acetate, Menadione Sodium Bisulﬁte Complex (Vitamin K), Calcium Pantothenate, Cobalt Carbonate, Copper Sulfate, Ferrous Sulfate, Manganese Oxide, Mangnesium Oxide, Zinc Oxide, Calcium Iodate.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.00 0.90 0.35 0.27 0.20 0.43 1.52 0.83 0.86 0.62 0.69 0.83

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.20 0.85 0.64 0.28 0.21 292.00 66.00 108.00 17.00 0.64 1.91

Vitamin Concentration

Vitamin A IU/g 8.30 IU/g 7.66 Vitamin D3 Alpha-Tocopherol IU/kg 100.00 Thiamine ppm 17.00 Riboflavin ppm 6.50 Niacin ppm 74.80 Pantothenic Acid ppm 32.20 Choline ppm 1888.00 Pyrodoxine ppm 10.20 Folic Acid ppm 3.12 Biotin ppm 0.34 Vitamin B12 Mcg/kg 44.00 Vitamin K ppm 3.54 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

4.01

Particle Size Wafer

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

18.0% 6.0% 4.0% 8.0%

Storage • •

Best if used within nine (9) months from date of manufacture. Sealed bags should be stored in a well-ventilated area in a cool, dry area away from direct sun light. Keep bags elevated from direct contact with ﬂoors and walls, protected from moisture and pests. 411005 08/10

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Rodent RQ 18-9 for Rats and Mice Colonies The Rodent RQ 18-9 (18% protein, 9% fat) diet is formulated to provide the nutrients required for maximum reproductive performance by rat and mice colonies maintained in conventional environments and where postpartum matings routinely occur.

Ingredients

Wheat, Corn, Soybean Meal, Wheat Middlings, Full Fat-Extruted Soy, Wheat Feed Flour, Soy Oil, Fish Meal, Dicalcium Phosphate, Limestone, Salt, Choline Chloride, DL Methionine, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E), Folic Acid, Niacin, Riboﬂavin, Thiamine, Vitamin B12 Supplement, Pyridoxine Hydrochloride, Biotin, Vitamin A Acetate, Menadione Sodium Bisulﬁte Complex (Vitamin K), Calcium Pantothenate, Cobalt Carbonate, Copper Sulfate, Ferrous Sulfate, Manganese Oxide.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.14 0.94 0.45 0.31 0.24 0.44 1.37 0.80 0.85 0.63 0.67 0.88

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.13 0.69 0.74 0.25 0.20 341.00 63.00 143.00 17.00 0.83 2.72

Vitamin Concentration

Vitamin A IU/g 4.45 Vitamin D3 IU/g 4.73 Alpha-Tocopherol IU/kg 109.00 Thiamine ppm 12.50 Riboflavin ppm 4.00 Niacin ppm 55.40 Pantothenic Acid ppm 23.30 Choline ppm 1500.00 Pyrodoxine ppm 7.50 Folic Acid ppm 2.30 Biotin ppm 0.20 Vitamin B12 Mcg/kg 27.50 Vitamin K ppm 2.00 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

4.24

Particle Size Wafer

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

18.0% 9.0% 4.0% 8.0%

Storage • •

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Rodent RQ 19-11 for Rats and Mice Colonies The Rodent RQ 19-11 (19% protein, 11% fat) diet is formulated to provide the nutrients required for maximum reproductive performance by rat and mouse colonies maintained in conventional environments, and where postpartum matings routinely occur in rodent strains with requirements for increased concentrations of dietary protein and energy.

Ingredients

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.25 1.04 0.47 0.31 0.25 0.48 1.48 0.88 0.93 0.68 0.73 0.95

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.13 0.68 0.79 0.24 0.21 342.00 62.00 140.00 17.00 0.82 2.71

Vitamin Concentration

Vitamin A IU/g 4.49 IU/g 4.74 Vitamin D3 Alpha-Tocopherol IU/kg 113.00 Thiamine ppm 12.30 Riboflavin ppm 4.00 Niacin ppm 51.30 Pantothenic Acid ppm 23.00 Choline ppm 1534.00 Pyrodoxine ppm 7.50 Folic Acid ppm 2.46 Biotin ppm 0.25 Vitamin B12 Mcg/kg 28.80 Vitamin K ppm 2.07 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

4.25

Particle Size Wafer

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

19.0% 11.0% 4.0% 8.0%

Storage • •

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Rodent RQ 22-5 for Rats and Mice Colonies The Rodent RQ 22-5 (22% protein, 5% fat) diet is specifically formulated for maintenance, growth, reproduction and lactation of conventional rats and mice.

Ingredients

Corn, Wheat, Soybean Meal, Wheat Middlings, Corn Gluten Meal, Fish Meal, Alfalfa Meal, Soy Oil, Brewers Dried Yeast, Ameribond, Dicalcium Phosphate, Salt, Limestone, Choline Chloride, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E), Folic Acid, Niacin, Riboﬂavin, Thiamine Mononitrate, Vitamin B12 Supplement, Pyridoxine Hydrochloride, Biotin, Vitamin A, Acetate, Menadione Sodium Bisulﬁte Complex (Vitamin K), Calcium Pantothenate, Cobalt Carbonate, Copper Sulfate, Ferrous Sulfate, Manganese Oxide, Zinc Oxide, Calcium Iodate.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.30 1.10 0.42 0.43 0.25 0.53 1.98 1.01 1.08 0.80 0.84 1.12

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

0.91 0.82 0.83 0.30 0.20 274.00 62.00 105.00 16.00 0.66 1.90

Vitamin Concentration

Vitamin A IU/g 8.79 IU/g 7.10 Vitamin D3 Alpha-Tocopherol IU/kg 105.00 Thiamine ppm 16.50 Riboflavin ppm 6.20 Niacin ppm 70.00 Pantothenic Acid ppm 31.00 Choline ppm 1853.00 Pyrodoxine ppm 10.00 Folic Acid ppm 3.28 Biotin ppm 0.32 Vitamin B12 Mcg/kg 40.00 Vitamin K ppm 3.40 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

4.07

Particle Size Wafer

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

22.0% 5.0% 4.0% 7.0%

Storage • •

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Rodent RQ 22-9 for Rats and Mice Colonies The Rodent RQ 22-9 (22% protein, 9% fat) diet is specifically formulated for reproduction and lactation of both rats and mice, and strains of rodents that have higher energy requirements. It is recommended for use in production and research rodent colonies.

Ingredients

Wheat, Corn, Soybean Meal, Wheat Middlings, Wheat Flour, Soy Oil, Fish Meal, CornGluten Meal, Alfalfa Meal, Brewers Dried Yeast, Dicalcium Phosphate, Ameribond, Salt,Limestone, Choline Chloride, Vitamin D3 Supplement, dl-AlphaTocopheryl Acetate (Vitamin E), Folic Acid, Niacin, Riboﬂavin, Thiamine Mononitrate, Vitamin B12 Supplement, Pyridoxine Hydrochloride, Biotin, Vitamin A , Acetate, Menadione Sodium Bisulﬁte Complex (Vitamin K), Calcium Pantothenate, Cobalt Carbonate, Copper Sulfate, Ferrous Sulfate, Manganese Oxide.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.31 1.13 0.41 0.35 0.26 0.52 1.94 1.01 1.09 0.78 0.85 1.12

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

0.91 0.77 0.77 0.27 0.18 270.00 60.00 102.00 16.00 0.66 1.92

Vitamin Concentration

Vitamin A IU/g 8.60 IU/g 7.10 Vitamin D3 Alpha-Tocopherol IU/kg 115.00 Thiamine ppm 15.30 Riboflavin ppm 6.30 Niacin ppm 65.90 Pantothenic Acid ppm 31.00 Choline ppm 1799.00 Pyrodoxine ppm 9.00 Folic Acid ppm 3.00 Biotin ppm 0.34 Vitamin B12 Mcg/kg 39.00 Vitamin K ppm 3.40 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

4.29

Particle Size Wafer

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

22.0% 9.0% 4.0% 7.0%

Storage • •

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Rodent

NIH-07 Open Formula for Rats and Mice Colonies The Rodent NIH-07 Open Formula diet is formulated for reproduction and lactation of both rats and mice. It has been used at the National Institutes of Health as the standard reference diet for biological and biomedical research. It is also recommended for use in production and research rodent colonies.

Dietary Ingredient Composition (% by Weight) Ground No. 2 Yellow Corn Ground Whole Wheat Soybean Meal (47.5%) Fish Meal (60%) Wheat Middlings

24.28 23.00 12.00 10.00 10.00

Dried Skim Milk Corn Gluten Meal (60%) Alfalfa Meal (4%) Soy Oil Brewers Dried Yeast

5.00 3.00 4.00 2.50 2.00

Dried Molasses Dicalcium Phosphate Limestone Salt Premixes

1.50 1.25 0.50 0.50 0.47

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.31 1.29 0.48 0.33 0.26 0.54 1.96 1.08 1.07 0.80 0.90 1.19

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.19 0.96 0.83 0.31 0.19 288.00 67.00 103.00 16.00 0.68 2.00

Vitamin Concentration

Vitamin A IU/g 8.77 IU/g 7.10 Vitamin D3 Alpha-Tocopherol IU/kg 104.00 Thiamine ppm 15.70 Riboflavin ppm 7.00 Niacin ppm 70.00 Pantothenic Acid ppm 32.70 Choline ppm 1874.00 Pyrodoxine ppm 10.00 Folic Acid ppm 3.00 Biotin ppm 0.36 Vitamin B12 Mcg/kg 48.00 Vitamin K ppm 3.50 Gross Energy

Kcal/gm

4.08

Particle Size

Packaging 2 - 25 lb. bags per box (Irradiated & Non-Irradiated) 50 lb., 3-ply paper bags

Wafer or Meal

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

22.5% 5.0% 4.0% 7.5%

Storage • •

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Rodent

NIH-31 Open Formula Auto for Maintenance & Growth The NIH-31 Open Formula Autoclavable diet is formulated for maintenance, growth, reproduction, and lactation of both rats and mice. It contains additional amounts of vitamins to compensate for losses that occur during steam sterilization. It has been used at the National Institutes of Health as the standard reference diet for biological and biomedical research. It is also widely used in commercial rat and mouse production colonies.

Ingredients Ground No. 2 Yellow Corn Ground Whole Wheat Ground Whole Oats Wheat Middlings Fish Meal (60%) Soybean Meal (47.5%)

21.00 35.37 10.00 10.00 9.00 5.00

Soybean Oil Alfalfa Meal (17%) Corn Gluten Meal (60%) Brewers Dried Yeast Dicalcium Phosphate

1.50 2.00 2.00 1.00 1.50

Limestone Salt Premixes

1.50 0.50 0.63

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.02 0.91 0.36 0.28 0.20 0.41 1.48 0.79 0.82 0.63 0.67 0.90

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.11 0.93 0.58 0.28 0.22 206.00 58.00 152.00 17.50 0.72 2.00

Vitamin Concentration

Vitamin A IU/g 25.90 IU/g 4.00 Vitamin D3 Alpha-Tocopherol IU/kg 44.00 Thiamine ppm 77.00 Riboflavin ppm 7.60 Niacin ppm 83.20 Pantothenic Acid ppm 38.00 Choline ppm 1916.00 Pyrodoxine ppm 10.60 Folic Acid ppm 1.56 Biotin ppm 0.30 Vitamin B12 Mcg/kg 55.80 Vitamin K ppm 22.20 Gross Energy

Packaging 30 lb., 3-ply paper bags

Kcal/gm

3.97

Particle Size 1/2” Pellet or Wafer

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

18.0% 4.0% 5.0% 8.0%

Storage • •

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Rodent

NIH-31 Modified Auto for Rats and Mice Colonies Rodent NIH-31M Auto is an open formula, autoclavable diet with a higher energy concentration than NIH-31diet in order to promote maximum reproduction and growth in both rats and mice reared as models in biomedical research.

Dietary Ingredient Composition (% by Weight) Ground No. 2 Yellow Corn Ground Whole Wheat Ground Whole Oats Wheat Middlings Fish Meal (60%)

20.00 35.17 10.00 10.00 9.00

Soybean Meal (47.5%) Soybean Oil Alfalfa Meal (17%) Corn Gluten Meal (60%) Brewers Dried Yeast

5.00 2.50 2.00 2.00 1.00

Dicalcium Phosphate Limestone Salt Premixes Lysine dl-Methionine

1.50 0.50 0.50 0.63 0.10 0.10

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.01 0.99 0.46 0.27 0.20 0.41 1.47 0.79 0.82 0.63 0.67 0.90

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.11 0.93 0.57 0.28 0.22 206.00 58.00 152.00 17.00 0.70 1.97

Vitamin Concentration

Vitamin A IU/g 25.90 IU/g 4.10 Vitamin D3 Alpha-Tocopherol IU/kg 46.80 Thiamine ppm 76.90 Riboflavin ppm 7.60 Niacin ppm 82.80 Pantothenic Acid ppm 38.10 Choline ppm 1880.00 Pyrodoxine ppm 10.60 Folic Acid ppm 1.56 Biotin ppm 0.29 Vitamin B12 Mcg/kg 56.00 Vitamin K ppm 22.00 Gross Energy

Packaging 30 lb., 3-ply paper bags

Kcal/gm

4.02

Particle Size Wafer

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

18.0% 5.0% 5.0% 8.0%

Storage • •

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Phytoestrogen

Reduced I 22.5-5

for dietary concentrations

This rodent diet is specifically formulated for studies where dietary concentrations of naturally occurring phytoestrogens, such as genistein, daidzein or glycitein, can influence experimental results. The formulation of this diet is a modification of the NIH-07 diet where ingredients such as soybean products and alfalfa meal, known to contain these hormones, are not used.

Ingredients

Corn , Wheat, Fish Meal, Wheat Middlings, Lactic Casein, Dried Skim Milk, Corn Gluten Meal, Corn Oil, Brewers Dried Yeast, Dry Molasses, Limestone, Salt, Dicalcium Phosphate, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E), Folic Acid, Niacin, Riboﬂavin, Thiamine, Vitamin B12 Supplement, Pyridoxine, Menadione Sodium Bisulﬁte Complex (Vitamin K) Vitamin A Palmitate, Choline, Biotin Calcium, Pantothenate, Cobalt Carbonate, Copper Sulfate, Ferrous Sulfate, Manganese Oxide.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.14 1.43 0.59 0.27 0.25 0.59 2.17 1.19 1.13 0.95 0.95 1.37

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.10 0.90 0.52 0.30 0.16 300.00 64.00 100.00 13.00 0.60 1.98

Vitamin Concentration

Vitamin A IU/g 6.60 IU/g 7.10 Vitamin D3 Alpha-Tocopherol IU/kg 51.20 Thiamine ppm 15.70 Riboflavin ppm 6.80 Niacin ppm 69.50 Pantothenic Acid ppm 30.70 Choline ppm 1563.00 Pyrodoxine ppm 10.00 Folic Acid ppm 2.80 Biotin ppm 0.23 Vitamin B12 Mcg/kg 48.00 Vitamin K ppm 3.10 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

4.10

Particle Size 1/2” Pellet

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

22.5% 5.0% 3.5% 7.5%

Storage • •

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Phytoestrogen

Reduced II 18-5

for dietary concentrations

This rodent diet is specifically formulated for studies where dietary concentrations of naturally occurring phytoestrogens, such as genistein, daidzein or glycitein, can influence experimental results. The formulation of this diet is a modification of the NIH-31 Modified diet where ingredients such as soybean products and alfalfa meal, known to contain these hormones, are not used.

Ingredients

Corn, Wheat, Fish Meal, Wheat Middlings, Oats, Corn Gluten Meal, Corn Oil, Brewers Dried Yeast, Limestone, Salt, Dicalcium Phosphate, Vitamin D3 Supplement, dl-AlphaTocopheryl Acetate (Vitamin E), Folic Acid, Niacin, Riboﬂavin, Thiamine, Vitamin B12 Supplement, Pyridoxine, Menadione Sodium Bisulﬁte Complex (Vitamin K) Vitamin A Palmitate, Choline, Biotin Calcium Pantothenate, Cobalt Carbonate, Copper Sulfate, Ferrous Sulfate, Manganese Oxide, Magnesium Oxide, Zinc Oxide, Calcium Iodate.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.94 0.83 0.39 0.27 0.18 0.41 1.60 0.76 0.83 0.65 0.65 0.88

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.12 0.95 0.48 0.29 0.21 300.00 61.00 154.00 18.00 0.60 1.98

Vitamin Concentration

Vitamin A IU/g 24.90 IU/g 4.20 Vitamin D3 Alpha-Tocopherol IU/kg 47.00 Thiamine ppm 77.70 Riboflavin ppm 7.30 Niacin ppm 85.00 Pantothenic Acid ppm 37.70 Choline ppm 1857.00 Pyrodoxine ppm 10.80 Folic Acid ppm 1.64 Biotin ppm 0.26 Vitamin B12 Mcg/kg 57.10 Vitamin K ppm 22.00 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

4.02

Particle Size 1/2” Pellet

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

18.0% 5.0% 4.5% 7.5%

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Rodent NTP-2000 National Toxicology Program The Rodent NTP-2000 open formula diet is formulated specifically to meet the nutrient requirements of rats and mice involved in long-term toxicology studies supported by the National Toxicology Program.

Dietary Ingredient Composition (% by Weight) Ground Whole Wheat Ground No. 2 Yellow Corn Wheat Middlings Oat Hulls Alfalfa Meal Solka Floc-40

23.00 22.44 15.00 8.50 7.50 5.50

Soy Protein Concentrate Fish Meal (60%) Soybean Oil Corn Oil Brewers Dried Yeast Calcium Carbonate

4.00 4.00 3.00 3.00 1.00 0.90

Dicalcium Phosphate Salt DL Methionine Premixes

0.40 0.30 0.20 1.26

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.75 0.65 0.44 0.21 0.17 0.32 1.04 0.58 0.59 0.45 0.52 0.66

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

0.99 0.56 0.69 0.19 0.22 132.00 47.00 44.00 10.00 0.12 0.33

Vitamin Concentration

Vitamin A IU/g 9.70 IU/g 1.00 Vitamin D3 Alpha-Tocopherol IU/kg 145.00 Thiamine ppm 9.30 Riboflavin ppm 5.70 Niacin ppm 54.90 Pantothenic Acid ppm 19.00 Choline ppm 2326.00 Pyrodoxine ppm 11.00 Folic Acid ppm 1.80 Biotin ppm 0.32 Vitamin B12 Mcg/kg 53.50 Vitamin K ppm 1.60 Gross Energy

Packaging 2 - 25 lb.bags per box

Kcal/gm

4.16

Particle Size Wafer or Meal

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

13.0% 8.0% 12.0% 8.0%

Storage This product has been treated with radiation. IRRADIATED

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Rabbit Diets

General Information

Rabbit Research Quality (RQ) Diets Zeigler Rabbit RQ Diets are ﬁxed formula diets that provide those nutrients required for maximum reproductive performance in rabbit production and research colonies maintained in Speciﬁc Pathogen Free (SPF) or conventional environments. Zeigler Rabbit RQ Diets are “closed formula” or proprietary-formula diets in that the quantitative ingredient concentrations in these diets are not readily available.

The National Institutes of Health (NIH) Open Formula Diets Zeigler Rabbit NIH Open Formula Diets were developed at the NIH as standard reference diets for use throughout the biomedical research community. These diets are referred to as “Open Formula” Diets because the quantitative ingredient composition is readily available. Zeigler was the ﬁrst company to make the NIH diets commercially available approximately 35 years ago.

Utilization of Rabbit Diets Shelf Life:

Zeigler natural ingredient rabbit diets are formulated for a shelf life of six months after the date of manufacture, which is consistent with the recommendations in the NIH “Guide for the Care and Use of Laboratory Animals.” Laboratory animal diets should be stored in cool, dry areas where the humidity is low in order to realize maximum shelf life. In addition, feed storage areas should be free of vermin and hazardous materials in order to prevent biological or chemical contaminations.

Sizes:

Zeigler rabbit diets are most frequently manufactured in pellets 5/32” in diameter and 5/16”- 5/8” long. These diets can also be manufactured into pellets of various other sizes to accommodate speciﬁc customer requirements.

Packaging:

Zeigler rabbit diets are packaged in 3-ply laminated paper bags of varying weights to accommodate customer requirements.

Zeigler rabbit diets can be fed ad libitum. The amount of feed consumed by rabbits will Feeding Instructions: vary according to age, size and reproductive status. For instance, a pregnant rabbit will

consume from 150-200 grams per day and during lactation this may increase to 200-400 grams per day. Limited feeding is acceptable to prevent obesity.

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Rabbit RQ 16-3 for production and research The Rabbit RQ 16-3 (16% protein, 3% fat) diet is formulated to meet maintenance, growth, reproduction, and lactation requirements of rabbits. It is recommended for use in production and research rabbit colonies.

Ingredients

Wheat Middlings, Dehydrated Alfalfa Meal, Soybean Hulls, Dehulled Soybean Meal, Corn, Soy Oil, Dicalcium Phosphate, Salt, Limestone, Lignin Sulfonate, dl-Methionine, Calcium Carbonate, Ferrous Carbonate, Manganous Oxide, Copper Oxide, Copper Sulfate, Zinc Oxide, Calcium Iodate, Sodium Selenite, Copper Sulfate, Vitamin D3 Supplement, dl-Tocopheryl Acetate (Vitamin E Supplement), Folic Acid, Niacin Supplement, Riboﬂavin Supplement, Thiamine Mononitrate, Vitamin B12 Supplement, Pyridoxine Hydrochloride, Biotin, Vitamin A Acetate, Menadione Sodium Bisulﬁte Complex (Vitamin K), Calcium Pantothenate, L-Lysine.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.97 0.80 0.40 0.27 0.25 0.39 1.29 0.80 0.81 0.51 0.67 0.88

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

0.91 0.77 0.77 0.27 0.18 270.00 60.00 102.00 16.00 0.66 1.92

Vitamin Concentration

Vitamin A IU/g 22.7 IU/g 1.32 Vitamin D3 Alpha-Tocopherol IU/kg 139.00 Thiamine ppm 12.00 Riboflavin ppm 7.67 Niacin ppm 57.80 Pantothenic Acid ppm 22.30 Choline ppm 2091.00 Pyrodoxine ppm 9.80 Folic Acid ppm 3.36 Biotin ppm 0.32 Vitamin B12 Mcg/kg 6.73 Vitamin K ppm 4.47 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

3.52

Particle Size 5/32 in

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

16.0% 3.0% 15.0% 8.0%

Storage • •

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Rabbit RQ Hi-Fiber 14-2.5 general lab maintenance Rabbit RQ Hi-Fiber 14-2.5 (14% protein, 2.5% fat) diet is formulated to meet the nutrient requirements for the maintenance of laboratory rabbits. This diet is a complete diet when fed ab libitum to rabbits involved in long-term studies.

Ingredients

Alfalfa meal, Oat Hulls, Wheat Middlings, Oats, Soybean Meal, Dried Whey, Dicalcium Phosphate, Soybean Oil, Brewers Dried Yeast, Monosodium Phosphate, Salt, dl-Methionine Vitamin D3 supplement, dl-Alpha Tocopheryl Acetate (Vitamin E), Folic Acid, Niacin, Riboﬂavin, Thiamine Mononitrate, Vitamin B12 Supplement, Pyridoxine Hydrochloride, Menadione Sodium Bisulﬁte Complex (Vitamin K) Vitamin A Palmitate, Choline Chloride, Calcium Pantothenate, Cobalt Carbonate, Copper Sulfate, Ferrous Sulfate, Manganese Oxide, Zinc Oxide, Calcium Iodate.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.78 0.77 0.35 0.25 0.28 0.32 1.14 0.72 0.70 0.49 0.63 0.78

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.23 0.73 1.70 0.34 0.29 446.24 53.00 66.00 20.00 1.73 1.35

Vitamin Concentration

Vitamin A IU/g 37.00 IU/g 2.20 Vitamin D3 Alpha-Tocopherol IU/kg 116.00 Thiamine ppm 9.70 Riboflavin ppm 11.80 Niacin ppm 53.00 Pantothenic Acid ppm 27.80 Choline ppm 2154.00 Pyrodoxine ppm 11.00 Folic Acid ppm 5.40 Biotin ppm 0.37 Vitamin B12 Mcg/kg 11.40 Vitamin K ppm 7.50 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

2.24

Particle Size 5/32 in

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

14.0% 2.5% 22.0% 10.0%

Storage • •

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Rabbit

NIH-09 Open Formula for Maintenance & Growth Rabbit NIH-09 Open Formula diet is formulated for maintenance, growth, and reproduction of conventional rabbits. It has been used at National Institutes of Health as the standard reference diet for rabbit production and in rabbit colonies involved in biological and biomedical research.

Ingredients Ground Barley Soybean Meal (47.5%) Oat Hulls Wheat Middlings Wheat Bran

14.70 13.00 22.50 6.00 7.00

Dried Whey Alfalfa Meal (17%) Brewers Dried Yeast Soy Oil Dried Molasses

2.00 28.10 1.00 0.50 1.50

Dicalcium Phosphate Limestone Salt Premixes

1.26 1.50 0.50 0.45

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.98 0.90 0.37 0.28 0.28 0.38 1.24 0.79 0.79 0.55 0.67 0.86

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.45 0.63 1.39 0.30 0.29 404.54 55.00 68.00 21.60 1.80 1.36

Vitamin Concentration

Vitamin A IU/g 22.60 IU/g 2.00 Vitamin D3 Alpha-Tocopherol IU/kg 86.00 Thiamine ppm 8.40 Riboflavin ppm 9.20 Niacin ppm 71.10 Pantothenic Acid ppm 23.60 Choline ppm 1717.00 Pyrodoxine ppm 10.70 Folic Acid ppm 4.50 Biotin ppm 0.36 Vitamin B12 Mcg/kg 11.00 Vitamin K ppm 5.40 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

2.37

Particle Size 5/32 in

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

16.5% 2.4% 16.8% 10.0%

Storage • •

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Rabbit

NIH-32 Open Formula Auto for Maintenance & Growth Rabbit NIH-32 Open Formula Auto is an autoclavable diet designed for maintenance, growth, and reproduction of rabbits. The diet contains higher concentrations of vitamins to compensate for losses that occur during steam sterilization. It has been used as a standard reference diet in rabbit colonies involved in biological and biomedical research.

Ingredients Ground Barley Soybean Meal (47.5%) Oat Hulls Wheat Middlings Wheat Bran

17.80 12.00 22.00 5.84 7.00

Dried Whey Alfalfa Meal (17%) Brewers Dried Yeast Soy Oil Dicalcium Phosphate

1.00 28.00 2.00 0.50 1.26

Limestone Salt Premixes

1.50 0.50 0.60

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.98 0.90 0.37 0.27 0.28 0.38 1.24 0.79 0.80 0.55 0.68 0.87

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.43 0.63 1.32 0.28 0.29 272.00 56.00 68.10 20.00 1.50 1.33

Vitamin Concentration

Vitamin A IU/g 27.00 IU/g 2.20 Vitamin D3 Alpha-Tocopherol IU/kg 87.90 Thiamine ppm 25.90 Riboflavin ppm 12.50 Niacin ppm 73.77 Pantothenic Acid ppm 26.40 Choline ppm 1711.00 Pyrodoxine ppm 16.00 Folic Acid ppm 4.42 Biotin ppm 0.68 Vitamin B12 Mcg/kg 22.50 Vitamin K ppm 13.30 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

2.15

Particle Size 5/32 in

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

16.0% 2.5% 15.8% 8.0%

Storage • •

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Guinea Pig Diets

General Information

Zeigler provides customers with a series of Guinea Pig Diets for use throughout the biomedical research community for both inbred strains and outbred stocks of guinea pigs.

Guinea Pig Research Quality (RQ) Diets Zeigler Guinea Pig RQ Diets are ﬁxed formula diets that provide those nutrients required for maximum reproductive performance in guinea pig production and research colonies maintained in conventional environments. Zeigler Guinea Pig RQ diets are “Closed Formula” or proprietary formula diets in that the quantitative ingredient concentrations are not readily available.

The National Institutes of Health (NIH) Open Formula Diets Zeigler Guinea Pig NIH Open Formula Diets were developed at the NIH as standard reference diets for use throughout the biomedical research community. These diets are referred to as “Open Formula” diets because the quantitative ingredient composition is readily available. Zeigler was the ﬁrst company to make the NIH diets commercially available approximately 35 years ago.

Utilization of Guinea Pig Diets Shelf Life:

Zeigler’s all natural ingredient guinea pig diets are formulated for a shelf life of six months after the date of manufacture, which is consistent with the recommendations in the NIH “Guide for the Care and Use of Laboratory Animals.” Guinea pig diets containing vitamin C use to have a 90-day shelf life, however, more stable forms of vitamin C, such as “STAY C”, are now available which have extended the shelf life of these products. Laboratory animal diets should be stored in cool, dry areas where the humidity is low in order to realize maximum shelf life. In addition, feed storage areas should be free of vermin and hazardous materials in order to prevent biological or chemical contaminations.

Sizes:

Zeigler Guinea Pig Diets are most frequently manufactured in pellets 5/32” in diameter and 5/16”- 5/8” long. These diets can also be manufactured into pellets of various sizes to accommodate speciﬁc customer requirements. Utilization of Guinea Pig Diets

Packaging:

Zeigler’s Guinea Pig Diets are packaged in 3-ply laminated paper bags of varying weight to accommodate customer requirements. Each bag is labeled with the date of manufacture.

Zeigler’s GuineaPig Diets are formulated for ad libitum feeding. The daily feed Feeding Instructions: consumption for adult guinea pigs will range from 25 to 50 grams per day.

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Guinea Pig RQ 18-4 for maintenance and growth Guinea Pig RQ 18-4 (18% protein, 4% fat) diet is formulated for maintenance, growth, reproduction, and lactation of conventionally reared guinea pigs.

Ingredients

Alfalfa Meal, Wheat Middlings, Oat Hulls, Wheat, Soybean Meal, Oats, Corn Gluten meal, Lecithin, Limestone, Dicalcium Phosphate, Salt, Soy Oil, Stay-C, Vitamin A Acetate, Vitamin D3 Supplement, dl- Alpha Tocopherol Acetate (Vitamin E), Menadione Sodium Bisulﬁte Complex (Vitamin K), Folic Acid, Choline Chloride, Niacin, d-Calcium Pantothenate, Pyridoxine Hydrochloride, Riboﬂavin Supplement, Thiamine Mononitrate, Vitamin B12 Supplement, Methionine Hydroxyanalogue, Copper Sulfate, Manganese Oxide, Zinc Oxide, Calcium Iodate.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

1.00 0.87 0.33 0.29 0.27 0.41 1.44 0.83 0.86 0.61 0.68 0.91

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.00 0.60 1.25 0.30 0.26 204.00 64.00 95.00 19.00 1.70 1.26

Vitamin Concentration

Vitamin A IU/g 20.60 IU/g 2.20 Vitamin D3 Alpha-Tocopherol IU/kg 68.00 Thiamine ppm 11.40 Riboflavin ppm 7.65 Niacin ppm 45.20 Pantothenic Acid ppm 26.30 Choline ppm 2067.00 Pyrodoxine ppm 10.50 Folic Acid ppm 6.33 Biotin ppm 0.41 Vitamin B12 Mcg/kg 11.20 Vitamin K ppm 6.93 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

2.60

Particle Size 5/32 in

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

18.0% 4.0% 14.5% 8.0%

Storage • •

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Guinea Pig NIH-34M for maintenance and growth Guinea Pig NIH-34M is an open formula diet is formulated for maintenance, growth, and reproduction of guinea pigs. It has been used at National Institutes of Health as the standard reference diet for biological and biomedical research.

Ingredients Ground Whole Oats Ground Whole Wheat Soybean Meal (47.5%) Alfalfa Meal (17%)

25.16 23.60 12.00 35.00

Dicalcium Phosphate Limestone Soy Oil

0.50 1.00 1.50

Salt Ascorbic Acid Premixes

0.75 0.09 0.40

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.97 0.83 0.31 0.30 0.26 0.35 1.25 0.77 0.80 0.58 0.63 0.85

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.15 0.42 1.32 0.37 0.23 303.21 50.00 80.00 16.40 1.73 1.26

Vitamin Concentration

Vitamin A IU/g 26.00 IU/g 2.20 Vitamin D3 Alpha-Tocopherol IU/kg 73.00 Thiamine ppm 8.60 Riboflavin ppm 8.80 Niacin ppm 42.60 Pantothenic Acid ppm 28.40 Choline ppm 1820.00 Pyrodoxine ppm 9.60 Folic Acid ppm 6.60 Biotin ppm 0.47 Vitamin B12 Mcg/kg 11.00 Vitamin K ppm 7.80 Vitamin C ppm 926.00 Gross Energy Kcal/gm 3.01

Packaging 50 lb., 3-ply paper bags

Particle Size 5/32 in

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

17.0% 3.4% 13.5% 8.5%

Storage • •

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Large Animal Model Diets General Information

In order to accommodate studies utilizing farm animal species as models in biomedical research, Zeigler manufactures and markets diets for swine and ungulates; sheep, goats, and other cloven hoofed species. These diets are manufactured in the drug-free facilities and with the same high quality ingredients that are used in other laboratory animal diets. These diets were originally developed at NIH and have been used extensively at that institution for the maintenance of these large animal species during long-term studies.

The National Institutes of Health (NIH) Open Formula Diets Zeigler large animal NIH Open Formula Diets were developed at NIH as standard reference diets for use throughout the biomedical research community. These diets are referred to as â&#x20AC;&#x153;Open Formulaâ&#x20AC;? diets because the quantitative ingredient composition is readily available. Zeigler was the ďŹ rst company to make the NIH diets commercially available approximately 35 years ago.

Utilization of Large Animal Diets Shelf Life:

Zeigler diets for large animal models are formulated for a shelf life of six months after the date of manufacture. These animal diets should be stored in cool, dry areas where the humidity is low in order to realize maximum shelf life. In addition, feed storage areas should be free of vermin and hazardous materials in order to prevent biological or chemical contaminations.

Sizes:

These diets are available in meal or pellet form to accommodate customer requirements. Utilization of Large Animal Diets

Packaging:

Zeigler diets for large animal models are available in 3-ply laminated paper bags or in bulk. Bags are labeled with the date of manufacture.

Diets for large animal species are formulated for limited feeding in accordance with Feeding Instructions: experimental protocols.

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Swine NIH-2004-2 for Domestic Swine Research Swine NIH-2004-2 diet is formulated for maintenance of domestic swine that are used as models in biomedical research.

Ingredients

Corn, Wheat, Soybean Meal, Ground-Extruded Whole soybeans, Oats, Soybean Hulls, Cane Molasses, Dicalcium Phosphate, Limestone, Salt, Wheat Middlings, dl-Alpha Tocopheryl Acetate (Vitamin E), Vitamin B12 Supplement, Riboﬂavin, Calcium Pantothenate, Vitamin A Acetate, Biotin, Pyridoxine Hydrochloride, Folic Acid, Niacin, Thiamine Mononitrate, Menadione Sodium Bisulﬁte Complex (Vitamin K), Vitamin D3 Supplement, Limestone, Magnesium Oxide, Ferrous Sulfate, Zinc Oxide, Manganese Oxide, Copper Sulfate, Cobalt Carbonate, Potassium Iodate, Sodium Selenite, L-Lysine, Choline Chloride, DL Methionine, Calcium Propionate.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.92 0.80 0.31 0.23 0.18 0.36 1.26 0.63 0.70 0.55 0.58 0.71

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.00 0.77 0.67 0.26 0.23 412.00 122.00 67.65 15.17 0.71 1.07

Vitamin Concentration

Vitamin A IU/g 6.00 IU/g 1.10 Vitamin D3 Alpha-Tocopherol IU/kg 89.06 Thiamine ppm 6.86 Riboflavin ppm 9.10 Niacin ppm 28.18 Pantothenic Acid ppm 19.80 Choline ppm 1534.00 Pyrodoxine ppm 10.60 Folic Acid ppm 6.80 Biotin ppm 0.30 Vitamin B12 Mcg/kg 77.00 Vitamin K ppm 0.60 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

3.21

Particle Size 5/32” Pellet

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

14.0% 4.0% 5.0% 8.0%

Storage • •

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Ungulate NIH-11 for Sheep & Goat Maintenance NIH-11 Ungulate diet is formulated for the maintenance of sheep and goats that are used as models in biomedical research, as well as other ungulates with nutrient requirements that are similar to these species.

Ingredients

Wheat Middlings, Alfalfa meal, Oat Hulls, Corn, Soybean Meal, Oats, Cane Molasses, Limestone, Salt, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E), Folic Acid, Niacin, Riboﬂavin, Menadione Sodium Bisulﬁte Complex (Vitamin K), Thiamine Mononitrate, Vitamin B12 Supplement, Pyridoxine Hydrochloride, Biotin, Vitamin A Acetate, Choline, Calcium Pantothenate, Cobalt Carbonate, Copper Sulfate, Zinc Oxide, Potassium Iodate, Sodium Sulfate.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.93 0.74 0.22 0.23 0.22 0.35 1.14 0.69 0.69 0.49 0.58 0.76

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.33 0.62 1.46 0.66 0.28 289.00 156.00 58.60 15.54 0.55 0.69

Vitamin Concentration

Vitamin A IU/g 24.20 IU/g 1.32 Vitamin D3 Alpha-Tocopherol IU/kg 80.40 Thiamine ppm 14.50 Riboflavin ppm 10.20 Niacin ppm 35.00 Pantothenic Acid ppm 14.70 Choline ppm 1904.95 Pyrodoxine ppm 10.70 Folic Acid ppm 2.90 Biotin ppm 0.38 Vitamin B12 Mcg/kg 50.00 Vitamin K ppm 3.20 Gross Energy

Packaging 50 lb., 3-ply paper bags

Kcal/gm

2.40

Particle Size 3/16” Pellet

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

14.0% 3.5% 12.5% 10.0%

Storage • •

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Aquatic Diets

General Information

For over 55 years, Zeigler has been producing foods with aquatic species, and today devotes significant research and development toward aquatic animal nutrition. These efforts have resulted in the development of specialized feeds for reproductive improvement, microcapsulated liquid foods for fish larvae, and highly digestible diets utilizing chelated minerals and stabilized vitamins to help increase nutritional bioavailability. All Zeigler Aquatic Research Diets utilize the latest technologies to help improve fecundity and overall fish health under laboratory tank conditions. Trials have demonstrated that embryo production can be increased significantly in Zebrafish fed the Zeigler Diet.

Utilization of Aquatic Diets Shelf Life:

Use within 3 months after opening. Unopened bags may be stored up to 2 years. To maximize shelf-life and maintain nutritive value, keep stored in a closed container in a cool, dry place away from sunlight. These products have been treated with radiation. Do not irradiate again.

Sizes:

Zebraﬁsh Diet is a sinking crumble (0.60 - 0.84 mm) and Xenopus Diet is a 3.0 mm ﬂoating pellet Utilization of Aquatic Diets

Packaging:

Irradiated resealable foil bag. Zebraﬁsh contains 16 oz per bag and Xenopus contains 12 oz per bag,, each packaged 24 foil bags per case.

Feeding See individual literature sheets for feeding instructions. Instructions:

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Adult Zebrafish Diet Promote Healthy Development Zeigler Adult Zebrafish Diet is a nutritionally complete diet for Zebrafish reared in the laboratory. It is specially formulated to promote healthy fin and tissue development and has demonstrated increased fecundity in laboratory conditions. The diet utilizes highly digestible ingredients which minimizes excreted waste thereby helping to maintain good water quality.

Ingredients

Fish Meal, Poultry By-Product Meal, Wheat Flour, Hydrolyzed Feather Meal, Fish Oil, Soy Protein Concentrate, Fish Protein Concentrate, Soy Protein Isolate, Soy Lecithin, Grain Distillers Dried Yeast, Yeast Culture, Yeast Extract, Calcium Propionate, Vitamin A Acetate, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E Supplement), Vitamin B12, Riboﬂavin, Niacin, Calcium Pantothenate, Menadione Sodium Bisulﬁte Complex (source of Vitamin K Activity), Folic Acid, Thiamine Mononitrate, Pyridoxine Hydrochloride, Biotin, Choline Chloride, Manganese Proteinate, Zinc Proteinate, Copper Proteinate, Calcium Iodate, Iron Proteinate, Cobalt Proteinate, Calcium Carbonate, Sodium Selenite, L-Ascorbyl-2-Polyphosphate (source of Vitamin C), Ethoxyquin (preservative), Astaxanthin.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

3.46 3.53 1.23 0.83 0.51 1.12 3.88 2.34 2.02 1.54 2.12 2.77

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

2.98 1.68 0.72 0.36 0.12 325.00 120.00 71.00 60.00 0.56 1.32

Vitamin Concentration

Vitamin A IU/g 25.60 IU/g 8.70 Vitamin D3 Alpha-Tocopherol IU/kg 446.70 Thiamine ppm 62.00 Riboflavin ppm 88.40 Niacin ppm 451.70 Pantothenic Acid ppm 189.20 Choline ppm 3855.00 Pyrodoxine ppm 60.20 Folic Acid ppm 18.49 Biotin ppm 1.32 Vitamin B12 Mcg/kg 241.90 Vitamin K ppm 18.40 Gross Energy

Packaging

Product Application Adult Zebraﬁsh Diet should be fed 2-3 times daily, adding the amount of food to each tank that can be consumed within 5 minutes. No supplementation is necessary for longterm maintenance of healthy zebraﬁsh in the laboratory.

24 - 16oz Pouches

IRRADIATED

3.89

Particle Size #1 Crumble (0.60-0.84mm)

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Moisture Ash

Storage This product has been treated with radiation.

Kcal/gm

• •

Minimum Minimum Maximum Maximum Maximum

55.0% 15.0% 1.5% 12.0% 8.0%

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Adult Xenopus Diet Nutritionally Complete Diet Zeigler Adult Xenopus Diet is a nutritionally complete diet used for Xenopus frogs reared in the laboratory. It is a highly palatable, floating diet formulated to minimize excreted waste and help maintain good water quality. The diet utilizes highly digestible ingredients, along with Stabilized Vitamin C and Brewers Yeast, to promote proper bone and tissue development.

Ingredients

Wheat, Poultry By-Product Meal, Wheat Middlings, Fish Meal, Hydrolyzed Feather Meal, Distillers Dried Grains with Solubles, Dehulled Soybean Meal, Fish Oil, L-Lysine, Calcium Propionate (Preservative), Choline Chloride, Vitamin A Acetate, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E Supplement), Vitamin B12 Supplement, Riboﬂavin, Niacin, Calcium Pantothenate, Menadione Sodium Bisulﬁte Complex (source of Vitamin K Activity), Folic Acid, Thiamine Mononitrate, Pyridoxine Hydrochloride, Biotin, dlMethionine, Manganese Proteinate, Zinc Proteinate, Copper Proteinate, Calcium Iodate, Iron Proteinate, Cobalt Proteinate, Calcium Carbonate, Sodium Selenite, Yeast Culture, L-Ascorbyl-2-Polyphosphate (source of Vitamin C).

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

2.46 2.17 0.76 0.93 0.34 0.74 2.77 1.72 1.44 1.96 1.43 1.48

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

1.73 1.34 0.60 0.36 0.19 296.00 102.00 77.00 42.00 0.40 1.41

Vitamin Concentration

Vitamin A IU/g 7.40 IU/g 2.00 Vitamin D3 Alpha-Tocopherol IU/kg 83.70 Thiamine ppm 26.75 Riboflavin ppm 29.37 Niacin ppm 195.50 Pantothenic Acid ppm 75.40 Choline ppm 2688.00 Pyrodoxine ppm 26.77 Folic Acid ppm 8.51 Biotin ppm 0.73 Vitamin B12 Mcg/kg 95.48 Vitamin K ppm 5.85 Gross Energy

Packaging

Product Application Feed once daily, adding the amount of food to each tank that can be consumed within 15 minutes. No supplementation is necessary for healthy frogs.

24 - 12oz Pouches

Storage IRRADIATED

2.96

Particle Size 3.0 mm

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Moisture Ash

This product has been treated with radiation.

Kcal/gm

• •

Minimum Minimum Maximum Maximum Maximum

40.0% 10.0% 3.5% 7.0% 12.0%

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Aquatox Fish Diet (Flake) Promote Healthy Development Zeigler Aquatox Fish Diet is a flake diet formulated as a completely balanced diet for maintenance and growth of fish used in aquatic toxicology research. It is also recommended as a stock diet for fish used in biological and biomedical research.

Ingredients

Fish Meal, Wheat Flour, Soy Flour, Corn Starch, Fish Protein Concentrate, Brewers Dried Yeast, Soy Lecithin, Sodium Alginate, Fish Oil, Emulsifier, Soy Protein Isolate, Choline Chloride, Vitamin A Acetate, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E Supplement), Vitamin B12 Supplement, Riboflavin, Niacin, Calcium Pantothenate, Menadione Sodium Bisulfite Complex (source of Vitamin K Activity), Folic Acid, Thiamine Mononitrate, Pyridoxine Hydrochloride, Biotin, L-Ascorbyl-2-Polyphosphate (source of Vitamin C), Manganese Sulfate, Zinc Sulfate, Copper Sulfate, Ethylenediamine Dihydriodide, TBHQ(preservative)

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

2.80 3.30 1.00 0.40 0.45 1.00 3.05 1.95 1.65 1.30 1.65 2.10

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

2.35 1.55 0.65 0.55 0.10 245.00 135.00 35.00 10.00 0.05 9.20

Vitamin Concentration

Vitamin A IU/g 14.50 IU/g 0.95 Vitamin D3 Alpha-Tocopherol IU/kg 780.00 Thiamine ppm 83.00 Riboflavin ppm 120.00 Niacin ppm 540.00 Pantothenic Acid ppm 240.00 Choline ppm 6665.00 Pyrodoxine ppm 72.00 Folic Acid ppm 20.00 Biotin ppm 1.25 Vitamin B12 Mcg/kg 100.00 Vitamin K ppm 24.00 Ascorbic Acid ppm 530.00 (as Stay-C)

Packaging 5 lb., 25 lb. box

Particle Size Flake

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Moisture Ash

Storage • •

Minimum Minimum Maximum Maximum Maximum

43.0% 13.0% 1.5% 10.0% 10.5%

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Research Diets

General Information

Zeigler’s Technical Service facility provides the biomedical research community with custom formulated laboratory animal diets. The following list of Zeigler Purified Diets may be modified to accommodate specific research requirements. Zeigler staff nutritionists are available to provide technical support in the development and use of custom research diets.

Vitamin Series

Stroke Research: Stroke Prone (SP) Animals

Vitamin A Deficient Rodent Diet Vitamin D Deficient Rodent Diet Vitamin D Deficient Dog Diet Tocopherol (E) Deficient Rodent Diet Vitamin K Deficient Chick Diet Thiamine (B1) Deficient Rodent Diet Folate Deficient Rodent Diet Pyridoxine (B6) Deficient Rodent Diet Riboflavin (B2) Deficient Rodent Diet

SP Rat Diet SP Rat Diet - 4% NaCl SP Rat Diet - 4% NaCl + 2.11% K SP Rat Diet - 6% NaCl SP Rat Diet - 6% NaCl + 2.11% K SP Rat Diet - 0.16% NaCl + 0.6% K SP Rat Diet - 0.16% NaCl + 2.11% K SP Rat Diet - Low Na, K SP Rat Diet - 8% NaCl + 2.11% K Mineral Series SP Rabbit Diet - 5% NaCl + 2.11% K

Mineral Series Copper Deficient Rodent Diet Iodine Deficient Rodent Diet Iron Deficient Rodent Diet Magnesium Deficient Dog Diet Na/K Deficient Rodent Diet Na/K Deficient Rabbit Diet Selenium Deficient Rodent Diet Sulfur Deficient Dog Diet Zinc Deficient Rodent Diet

Custom Research Diets

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Zeigler™ offers an extensive range of custom research diets. Please contact us for more information.

Utilization of Rabbit Diets Shelf Life:

Zeigler natural ingredient research diets are formulated for a shelf life of six months after the date of manufacture, which is consistent with the recommendations in the NIH “Guide for the Care and Use of Laboratory Animals.” Laboratory animal diets should be stored in cool, dry areas where the humidity is low in order to realize maximum shelf life. In addition, feed storage areas should be free of vermin and hazardous materials in order to prevent biological or chemical contaminations.

Sizes:

Meal, 1/16”, 3/32”, 1/8”, 5/32”, 3/16”, 3/8”and 1/2” Minimum order: 10 pounds per diet

Packaging:

Depending on the product and amount ordered, items may be packaged in a bag, box or ﬁberboard drum

08/10

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Purified

Rodent Diet AIN-76A for Rats and Mice Diets A committee of American Institute of Nutrition (AIN) members formulated and published the formulation for the AIN-76A puriﬁed rat and mouse diet. The rationale for publishing the formulation for a puriﬁed diet was to provide a standard reference diet that could be used throughout the biomedical research community by researchers who had limited knowledge and experience in diet formulation. The AIN-76A diet has been used successfully in biomedical research for the past 35 years.

Dietary Ingredient Composition (% by Weight) Granular Sugar Casein Lactic Corn Starch

50.00 20.00 15.00

Corn Oil Solka Floc-40 AIN-76 Mineral Mix

5.00 AIN-76 Vitamin Mix 5.00 DL- Methionine 3.50 Choline Bitartrate

1.00 0.30 0.20

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.70 1.48 0.86 0.06 0.21 0.52 1.76 1.14 0.96 0.98 0.78 1.34

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

0.52 0.44 0.38 0.11 0.05 34.25 36.75 59.34 6.73 0.02 0.21

Vitamin Concentration

Vitamin A IU/g 4.00 IU/g 1.00 Vitamin D3 Alpha-Tocopherol IU/kg 64.24 Thiamine ppm 5.90 Riboflavin ppm 6.29 Niacin ppm 30.15 Pantothenic Acid ppm 15.26 Choline ppm 1040.00 Pyrodoxine ppm 7.12 Folic Acid ppm 2.10 Biotin ppm 0.21 Vitamin B12 Mcg/kg 10.10 Vitamin K ppm 0.50 Gross Energy

Packaging Fiberboard Drum

Kcal/gm

4.34

Particle Size 1/2” Pellet or Meal

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

16.0% 5.0% 5.0% 4.0%

Storage • •

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Purified

Rodent Diet AIN-93G for Rats and Mice Diets AIN-93G was the ﬁrst of two open formulations published by the American Institute of Nutrition (AIN) committee in 1993 improving the laboratory standard, AIN-76A diet. AIN-93G is designed to accommodate the increased nutritional demands of rat or mouse growth as well as females who are pregnant or lactating, hense the “G” designation.

Dietary Ingredient Composition (% by Weight) Corn Sugar Casein Lactic Granular Sugar Dextrin

39.75 Solka Floc-40 20.00 AIN-93 Mineral Mix 10.00 AIN-93 Vitamin Mix 13.20

5.00 L-Cystine 3.50 Choline Bitartrate 1.00 Soy Oil

0.30 0.25 7.00

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.70 1.48 0.56 0.30 0.21 0.51 1.76 1.14 0.96 0.98 0.76 1.00

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

0.52 0.20 0.38 0.11 0.05 52.02 36.76 11.33 6.73 0.02 0.21

Vitamin Concentration

Vitamin A IU/g 4.00 IU/g 1.00 Vitamin D3 Alpha-Tocopherol IU/kg 75.00 Thiamine ppm 5.00 Riboflavin ppm 6.00 Niacin ppm 30.00 Pantothenic Acid ppm 15.00 Choline ppm 1000.00 Pyrodoxine ppm 6.00 Folic Acid ppm 2.00 Biotin ppm 0.20 Vitamin B12 Mcg/kg 25.00 Vitamin K ppm 0.90 Gross Energy

Packaging Fiberboard Drum

Kcal/gm

3.80

Particle Size 1/2” Pellet or Meal

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Moisture Ash

Minimum Minimum Maximum Maximum Maximum

17.9% 7.0% 4.8% 7.0% 4.2%

Storage • •

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Purified

Rodent Diet AIN-93M for Rats and Mice Diets AIN-93M is the second of two open formulations, published by the American Institute of Nutrition (AIN) committee in 1993 improving the AIN-76A standard diet. AIN-93M was structured to provide nutrients in concentrations required just to maintain adult rat or mouse populations, hence the “M” designation.

Dietary Ingredient Composition (% by Weight) Corn Starch Casein Lactic Dextrin AIN-93 Vitamin Mix

46.75 14.00 15.50 1.00

Granular Sugar Solka Floc-40 Soy Oil

5.00 3.50 1.00

AIN-93 Mineral Mix L- Cystine Choline Bitartrate

0.30 0.25 7.00

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.48 0.92 0.33 0.24 0.16 0.33 1.09 0.59 0.62 0.66 0.47 0.70

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

0.52 0.23 0.38 0.11 0.05 34.25 36.76 11.09 6.73 0.02 0.21

Vitamin Concentration

Packaging Fiberboard Drum

Kcal/gm

3.60

Particle Size 1/2” Pellet or Meal

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Moisture Ash

Minimum Minimum Maximum Maximum Maximum

12.6% 4.0% 4.8% 7.0% 3.9%

Storage • •

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Stroke Prone

Rodent Diet for Rats and Mice Colonies Stroke Prone Rodent Diet is an open formula, natural ingredient research diet for mice and rats. It typically serves as a Japanese-style base diet for trials testing sodium and potassium levels, particularly in (spSHR) stroke prone spontaneous hypertensive reactive rats. Zeigler nutritionists can customize this diet to your request.

Ingredients

Wheat Middlings, Corn, Rice Bran, Dehulled Soybean Meal, Wheat Bran, Fishmeal, Dried Skim Milk, Soy Oil, Salt, Limestone, Ferrous Sulfate, Magnesium Oxide, Manganese Oxide, Copper Sulfate, Cobalt Carbonate, Potassium Iodide, Choline Chloride, Vitamin D3 Supplement, dl-Alpha Tocopheryl Acetate (Vitamin E Supplement), Inositol, Niacin, Menadione Sodium Bisulﬁte Complex (Source of Vitamin K Activity), Riboﬂavin, Calcium Pantothenate, Vitamin A Acetate, Pyridoxine Hydrochloride, Thiamine Mononitrate, Biotin, Vitamin B12 Supplement, Folic Acid.

Calculated Nutrient Composition Amino Acid Concentration (% of total diet)

Arginine Lysine Methionine Cystine Tryptophan Glycine Histidine Leucine Isoleucine Phenylalanine Tyrosine Threonine Valine

0.75 0.70 0.23 0.18 0.15 0.58 0.28 0.97 0.55 0.52 0.39 0.46 0.61

Mineral Concentration

Calcium Phosphorous Potassium Sodium Magnesium Iron Zinc Manganese Copper Cobalt Iodine

% % % % % ppm ppm ppm ppm ppm ppm

0.86 0.50 0.55 0.39 0.15 178.00 24.00 75.95 8.36 2.54 2.72

Vitamin Concentration

Vitamin A IU/g 10.41 IU/g 2.02 Vitamin D3 Alpha-Tocopherol IU/kg 62.17 Thiamine ppm 12.07 Riboflavin ppm 12.47 Niacin ppm 84.93 Pantothenic Acid ppm 22.47 Choline ppm 1711.89 Pyrodoxine ppm 12.71 Folic Acid ppm 1.29 Biotin ppm 0.24 Vitamin B12 Mcg/kg 11.63 Vitamin K ppm 5.04 Gross Energy

Packaging Fiberboard Drum

Kcal/gm

1.86

Particle Size 1/2” Pellet or Meal

Guaranteed Analysis Crude Protein Crude Fat Crude Fiber Ash

Minimum Minimum Maximum Maximum

16.0% 3.8% 2.0% 5.5%

Storage • •

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Diet Certification Program

Zeigler provides a flexible certification program depending on the specific needs of the researcher. Contaminant specifications have been developed using guidelines established in conjunction with the National Toxicology Program. Certified diet customers are provided with results of the assay profile conducted on representative samples of purchased product. An independent laboratory is utilized for the analysis.

Nutrient

Protein, Fat, Fiber, Ash, Calcium, Phosphorus, Vitamin A, Thiamine (B1)

Mycotoxin Aflatoxcin (Total)

Pesticide Screen Chlorinated Hydrocarbons

Aldrin, Dieldrin, Endrin, DDT, DDE, DDD, HCB, Heptachlor, BHC, Heptachlor Epoxide, Methoxychlor, Toxaphene, Telodrin, Chlordane

Organo-Phosphates

Diazinon, Ethion, Malathion, Ethyl Parathion, Methyl Parathion, Ronnel, Trithion, Endosulfan 1 & 2, Endosulfan Sulfate.

Nitrosamines (ppb) Total, NDMA

Heavy Metals

Lead, Cadmimum, Arsenic, Mercury, Selenium

Phytoestrogens Nitrate & Nitrite BHA & BHT PCBâ&#x20AC;&#x2122;s Microbiological TPC, Coliform, E.Coli

Other Options:

Antibiotics: Estrogen; Vitamins (other); Minerals (other); Ochratoxin A, B; Patulin; Aflatoxin B1, B2, G1 and G2 fractions; T-2; Herbicides; Microbial (Salmonella, Total Spore Count, Total Mold Count, Pathogenic Fungi); Drugs and others.

Services and Capabilities ISO 9001 Certified

Zeigler manufacturing facilities in Gardners and East Berlin, Pennsylvania maintain ISO 9001: 2008 Registered Quality Systems. Standardizing services and manufacturing operations into an organized system is an added assurance that customers are always provided with the highest quality products.

Nutrient concentrations in Zeigler lab diets are controlled by the procurement of ingredients that contain acceptable specified nutrient concentrations. Reports of nutrient and contaminant assays conducted by independent laboratories are available upon request.

Technical Assistance

Drug-Free Manufacturing Facility

All Zeigler laboratory animal diets are manufactured in a drug-free facility. Strict manufacturing and feed storage procedures at this facility minimize cross contamination of products with undesirable constituents.

Technical support from Zeigler nutritionists and support staff is available to all customers. Assistance is available to resolve problems, explore alternative feeding methodologies, and develop new diet formulations to accommodate specific research requirements.

Ingredient Quality

Zeigler Customers

Feed ingredients used in Zeigler laboratory animal diets are procured only from vendors with a history of providing high quality ingredients. Ingredients are selected for nutrient consistency and low concentrations of recognized contaminants. Natural ingredients such as small grains and grain by products, alfalfa, and fishmeal are pre-assayed for contaminants to ensure that Zeigler laboratory animal diets are within the guidelines issued by various government agencies.

Fixed Formulas

Government agencies such as National Institutes of Health, Federal Drug Administration, Centers for Disease Control, and National Toxicology Program routinely use Zeigler laboratory animal diets. Zeigler also provides diets for numerous universities, AAALAC accredited animal breeders, pharmaceutical companies, and other institutions involved in biological or biomedical research.

International Activities

Zeigler nutritionists recognize that fixed formulations are required to minimize variables that may influence experimental results. Therefore, Zeigler lab diets are not formulated on a least-cost basis, nor altered without the knowledge of the customer.

page Zeigler is committed to the global biomedical research community through the establishment of various international relationships. Experience in feed export and technology transfer is available to assist the international community with various technical challenges related to the laboratory animal feed industry.

Drug-free manufacturing facility in East Berlin, Pennsylvania

Ordering Information To Place An Order:

For information on Zeigler Laboratory Animal Diets, contact our Customer Service Representative at: Zeigler Bros., Inc. 400 Gardners Station Road Gardners, PA 17324 USA Toll Free (800) 841-6800 Telephone (717) 677-6181 Fax (717) 677-6826 email: sales@zeiglerfeed.com

Delivery: Orders are priced FOB. Shipping charges will be prepaid and added to invoice. Stock items will be shipped within 1 week of purchase order receipt unless product is back ordered. Call customer service for delivery times on non-stock items.

Payment: Prepaid, unless other terms are noted upon order.

Going Green: Zeigler Bros., Inc. has made a commitment to operate in a more environmentally friendly way. Join us in our efforts to save paper and GO GREEN. Please elect to receive your invoices electronically by filling out the form on our website at www.zeiglerfeed.com/html/going_green.htm or speak with your Sales Specialist or Customer Service Representative.

Limited Warranty: All non-conformities must be reported in writing to Zeigler within 14 days of receipt of product for credit or replacement. Liability is limited to the cost of feed. There are no expressed warranties on product performance. Zeigler is not responsible for product which becomes unusable due to improper storage or handling.

Product Specifications: Most diets are available in meal and pellet form. Request appropriate form when placing order.

Analytical Service: Conforms to GLP/GMP guidelines and is available on a cost sharing basis

Certified Diets: Most diets can be certified as outlined in the â&#x20AC;&#x153;Laboratory Diet Certification Programâ&#x20AC;? section of catalog. Request appropriate certification when placing order.

Irradiated Diets: Most diets can be irradiated. Note request for irradiated product when placing order.

Zeigler Tradition Setting aside their orchestra careers touring as The Scotch Highlanders, two brothers, Ty and LeRoy Zeigler, moved their young families to rural Gettysburg, Pennsylvania, where they had just purchased a farm and a water-powered grist feed mill. The year was 1935. The first feeds were manufactured under the Conewago brand, named after the stream which powered their newly purchased mill. Their strong orientation to value, integrity and customer service was soon rewarded by the trust and business of many local farmers. Circa 1950â&#x20AC;&#x2122;s.

In 1967, the leadership passed to LeRoyâ&#x20AC;&#x2122;s son, Dr. Tom Zeigler, who transitioned the company from a manufacturer of farm feeds to an internationally recognized producer of specialized animal feeds. Beginning in the 1980s, the company began licensing nutritional and manufacturing technologies globally under its Zeigler brand. The Zeigler family tradition continues today, more than 75 years later, under the leadership of Tomâ&#x20AC;&#x2122;s sons, Tim and Matt Zeigler. With a renewed spirit toward technology and innovation, the brothers continue to uphold the company traditions of value and integrity, while striving to serve the changing needs of loyal customers.

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Product of

USA

Zeigler Bros., Inc. 400 Gardners Station Road Gardners, PA 17324 USA 717-677-6181 phone www.zeiglerfeed.com rev. 07/15