Gene Mutation AGB 121 Dept. Of AGB Veterinary College, Bangalore
Molecular Genetics Chromosome
Cell
DNA Nucleus
Nucleotides
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
DNA (deoxyribonucleic acid)
mRNA
Protein
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Properties of DNA
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Amino acid chain
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Properties of Genetic code
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Gene Mutation • Any heritable change in the genetic material • Sudden change in the gene or genotype involving the alteration in the genetic material
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Classification of gene mutation Based on Type of cells 1.Somatic mutation 2.Gametic mutation Molecular changes 1. Point mutation i. Same sense ii. Non sense iii. Mis sense iv. Frame shift
2.Structural Mutation i. Transition ii.Transversion Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Molecular mutation
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Point mutation i. Same sense ii. Non sense iii. Mis sense iv. Frame shift
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Frame shift mutation
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Structural Mutation • Transition mutation • Transversion mutation
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Induced Mutation • Radiation : a. Ionizing radiation i) Single strand Breakage - Repairable ii) Double strand breakage iii) Alteration in nucleotide bases b. Non ionizing radiation :U-V rays Thymine dimer causing distortion of double helix Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Induced Mutations
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
DNA damage
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Chemical mutagens • Base analog mutants: Produces mutation during DNA replication – 5 bromouracil is a base analog to Thymine A=T results in A=Bu • Chemical reactants: Produces mutagenesis in both replicating and non replicating DNA Nitrous acid convert the amino group of Adenine , Cytosine and Guanine to Keto group Hence Hydrogen bonding properties of the bases changes AT GC Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore
Thank you
Dr R Jayashree, Asst. Prof(AGB) Veterinary College, Bangalore