Introducing HALO-5 The First High-Performance 5-Micron Packing in the World
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Overview Introduction Particle Description Results: • Efficiency • Backpressure • Loadability
Features and Benefits Conclusions
Introduction HALO core-shell particles are unique high-purity silica particles for stable high efficiency packed columns that are capable of very fast separations with modest back pressures Original HALO particles are 2.7-microns with 90 Å pores HALO provides the efficiency of sub-2-micron particles at half the pressure New HALO-5 particles are ~ 5-microns with 90 Å pores HALO-5 provides the efficiency of 3-micron particles with the pressure of 5-micron particles
HALO® -5 Fused-Core Particles
~ 5 µm
0.6 µm
SEM of ~ 5 µm HALO Fused-core
Graphical representation of ~ 5 µm HALO Fused-core
Specifications for HALO-5 Particles
~ 5 μm overall diameter 0.6-μm-thick porous outer shell 90 Angstrom pores in porous shell Highly purified Type B silica Surface area ~100 m2/g Stable at pressures of at least 600 bar
HALO-5 Bonded Phases Available November 2012
- Phenyl-Hexyl - C8 C18 (octadecyl)
- ES-CN
Available February 2013 PFP (pentafluorophenylpropyl)
- HILIC -Penta-HILIC
Performance
• As predicted by theory, the smaller the particle, the smaller the plate height (e.g., the sharper the peaks) • The 4 µm and 2.2 µm particles are from R&D batches
• The reduced plate height, h (same plate height data divided by the particle size), provides a measure of packed bed quality. • The larger-particle HALO packings exhibit lower values for h, indicating more homogeneous packed beds. HALO(2.7) h = 1.5, HALO-5 h = 1.2!
Back Pressure Plots for Columns of Different Particle Sizes 600 2.7 m Halo fused-core 3 m totally porous 4 m Halo fused-core 5 m Halo fused-core 5 m totally porous Linear regression
500
Pressure, bar
400
300
200
•Columns: 150 x 4.6 mm, C18 •Mobile Phase: 50% ACN, 50% Water •Temperature: 30°C •Instruments: Agilent 1100 or Agilent 1200 SL
100
0 0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
Mobile Phase Flow Rate, mL/min
4.0
4.5
5.0
More Theoretical Plates at Lower Pressure 5 µm HALO fused-core
4 µm HALO fused-core
5 µm totally porous Plates/Pressure in bar
2.7 µm HALO fused-core
3 µm totally porous
1.8 µm totally porous 0
100
200
300
400
• When operated at optimum flow rates, HALO-5 columns provide the highest number of theoretical plates per unit of pressure
HALO-5 Columns Exhibit the Same Sample Loadability as 2.7-Micron HALO Columns and are Similar to 5-Micron Totally Porous Columns 35000
30000
25000
Plate Number
2.7 µm Fused-Core, 0.5 µm shell 20000
5 µm Fused-Core, 0.6 µm shell 5 µm Totally porous
15000
10000
5000 0.01
0.1
1
µg of 1-Cl-4-Nitrobenzene
10
100
Features and Benefits
Easy Transfer from Existing Methods
• HALO-5 Columns Can be Substituted for 5-Micron Totally Porous Columns to Provide Sharper Peaks and Shorter Analysis Times Under Identical Method Parameters
Obtain 5-Micron Benefits for 3-Micron Methods
• HALO-5 Columns Provide the Same Efficiency as 3-Micron Columns at about Half the Pressure
Get 3-micron Resolution at 5-micron Pressure with HALO-5 Rs = 1.9
2,000
2.7-micron Fused-core
1,800
1,600
3-micron TP Rs = 1.55
Resolution
1,400
5-micron Fused-core
1,200
Rs = 1.24
Rs = 1.2
1,000
5-micron TP 0,800
Rs = 0.9
0,600
0,400 0,00
0,50
1,00
1,50
2,00
2,50
Flow, ml/min
3,00
3,50
4,00
4,50
5,00
Comparable Selectivity with 2.7-Micron HALO PEAK IDENTITIES (in order): 1. Uracil 2. Resorcinol 3. Aniline 4. 4-Chloroaniline 5. Acetoacetanilide 6. Dimethylphthalate 7. Cinnamyl alcohol 8. 2,6-Dinitrotoluene 9. Tolbutamide 10. 4-Chloro-3-nitroanisole
• Methods are easily transferred from HALO 2.7-micron columns to HALO-5 Columns with only minor adjustment to mobile phase strength
Who Should Use HALO-5 Columns? Users who currently use 3- or 5-micron particle columns and want to increase their efficiency and speed Anyone wanting to transfer a method to a routine laboratory Users who want more speed or who want more separation power but are owners of pressure-limited HPLC instruments (400 bar max pressure)
Conclusions Regarding HALO-5 Columns Produce unusual column efficiency; reduced plate heights h of ~1.2 for small molecules making them the first high-performance 5-micron columns in the world. Efficiency and separation speed rivals that of 3-μm columns, but with about one-half the pressure. Thin porous outer shell results in superior mass transfer kinetics and better efficiency at high mobile phase velocities, especially for larger molecules. 2-μm porosity frits allow the convenience and ruggedness of columns with 5 μm particles. Strong particles allow pressures of at least 600 bar.