NEW! HALO ES-CN
•Moderately polar phase for alternate selectivity •Increased retention of polar compounds •Decreased retention of hydrophobic compounds
•Steric-Protected silane for low pH stability •Fully end-capped for neutral pH applications
Separation of mixed EPA explosive standards 8330 A and B on HALO C18 and ES-CN Phases Conditions: Columns: 4.6 x 100 mm, HALO C18, HALO ES-CN Mobile phase: 50/50:Methanol/water Flow: 1.25 mL/min; Pressure: about 300 bar Temperature: 30 deg. C. 4
Peak identities: 1) HMX, 2) RDX, 3) 1,3,5-TNB, 4) 1,3-DNT, 5) nitrobenzene, 6) Tetryl, 7) 2,4,6-TNT, 8) 4-amino-2,6,-DNT 9) 2-amino-4,6-DNT, 10) 2,6-DNT, 11) 2,4-DNT 12) 2-nitrotoluene, 13) 4-nitrotoluene, 14) 3-nitrotoluene
3 10, 11 1
5
Absorbance @ 254 nm
2
6
7
9 8 12
HALO C18
5
4, 3
12
13 14
7
10
2
3
9
8 2
1
14
11
HALO ES-CN
0
13
4 Minutes
6 1
5
6
7
8
Selectivity ES-CN vs. C18 Columns: 4.6 x 100mm Mobile phase: 40/60-water/MeOH for C18 50/50-water/MeOH for ES-CN Flow rate: 1.25 mL/min Pressure: 310 Bar Temperature: 30 째C.
6
Peaks: 1) resourcinol 2) benzyl alcohol 3) phenylacetonitrile 4) 1-indanol 5) 3,4-dinitrotoluene 6) 2,3-dinitrotoluene
1 5
3 2
7
Absorbance @ 254 nm
HALO C18
8
9
7) 2,4-dinitrotoluene 8) anisole 9) 1-chloro4-nitrobenzene 10) toluene i = impurity
4 i
10
i
1 3
2 HALO ES-CN
8
1
5
7
4 10
i 0
6 9
2
3 Minutes
4
5
6