Mir trace elements

The ultimate…skin cell nutrition

The innovative cell growth accelerator at cellular level for sustainability of your skin youthfulness TRACE ELEMENTS - MITONUTRIENTS http://mirworldwide.net

TRACE ELEMENTS - MITONUTRIENTS

What is cell?

Cell is the smallest unit of all living organisms which can express all the life characteristics. This little miracle thing involves in forming skin, muscles, bone and human organs. Cell is also the major component of human body that is organized to form and repair organs.

Endoplasmic reticulum Ribosome

Nucleus

Golgi Complex

How do we lose our youthfulness?

1. Losing necessary nutrients for healthy

skin and it depends on how perfect the

cells can perform.

2. Losing balance of hormones which are

necessary to activate body functional

system.

3. Losing efficiency of anti-oxidant which

is the main cause of cell damage.

4. Losing immune system.

Cell membrane

Mitochondria

Cytoplasm Lysosome

WHAT ARE TRACE ELEMENTS MITONUTRIENTS?

Mitronutrients is liquid nutrient for growing cells. It consists of 4 significant elements.

1. Trace elements from deep marine that are necessary minerals for cell growth.

2. Amino Acids : L-arginine HCI 3. Viatamins : Folic Acid 4. Growth factor for stimulating cell growth.

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Thus, MIR created the latest innovative Trace

Elements - Mitronutrients skincare range for sustainability

of skin youthfulness by slowing down cell deterioration at the cellular level as the following details Marine minerals from spring water of Atlantic coats in France.

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Marine spring water enriches with mineral much higher than sea water.

From the table, Silica, Calcium, Magnesium, Phosphate, Iron and Manganese are founded in marine water higher than sea water while sea water consists of the high level of Nitrites. The mineral intensity increases following earth crust thickness.

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Relation between skin structure and types of mineral for skin treatment.

Skin is made up of three successive levels.

Epidermis

Dermis Hypodermis !

1. Epidemis is a thin outer portion, minerals can be absorbed quickly. Minerals that required for this skin level are Zinc, Sodium, Potassium, Calcium and Iron.

2. Dermis is the layer underneath epidermis, it represents for 90% thickness of overall skin’s structure. Minerals that required for this skin level are Silica, Copper, Iron, Sodium, Phosphorus, and Zinc.

3. Hypodermis mainly contains adipose tissue. http://mirworldwide.net

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Functions of minerals HYDRATION Magnesium Potassium Sodium

COLLAGEN SYNTHESIS Calcium Magnesium Silicium

SENSITIVE AND REACTIVE SKIN Manganese Zinc ANTI FREE RADICALS Selenium Copper

BRITTLE HAIR & NAIR Silicium Copper

Magnesium, Potassium and Sodium help hydration reaction, maintains the water balance in cell and keeps skin moisture.

Manganese and Zinc provide the healthy and complete newborn cell.

Selenium and Copper enhance antioxidant and reduce factors that promote wrinkles.

Calcium, Magnesium and Selenium increase natural collagen for skin firming

and elasticity.

Silica and Copper create keratin which keeps hair and nails moist and healthy protecting skin from damage.

Stimulation of skin cells viability

Cell viability (% of the control)

Growing human fibroblast cells in marine water for 24 hours. The result indicates that marine water can increase 53% liveliness of skin cells.

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180 160 140 120 100 80 60 40 20 0

Control 0,25 2,5 25 Earth Marine Water (%) http://mirworldwide.net

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Human fibroblasts incubation in saline buffer during 24 hours

Improvement of skin cohesion

!

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Culture medium without minerals.

Saline buffer (NaCl 0.9 %) without calcium and without magnesium.

!

Saline buffer containing calcium and magnesium.

Thanks to its richness in Ca and Mg of Earth Marine which water improves epidermis cohesion.

Anti-Free Radical Activity

Earth marine water prevents skin from free radicals attacks.

Anti-free radical Activity (% in hibtion)

80 70 60 50 40

30 20 10 0

Demineralized Themal Mountain Sea Water Earth Marine water Water Spring Water Water

Free radicals generated by xanthine/xanthine oxidase.

Photo-Aging O2 OH

Proteins

UVA

Free radicals

H2O2

Mechanism of Photo-Aging

and Free Radical OH H 2O 2 O2

DNA

Mutation

Oxidation

When skin exposes to sunlight it causes the mutation in DNA level protein becomes oxidant thus skin cells change into wrinkles and pigmentary spots.

PHOTO-AGEING wrinkles pigmentary spots

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Protection against cigarette smoke Cell viability (% of the control)

Earth Marine Water has 47% protection against cigarette smoke.

110 100 90 80 70 60 50 40

Control

Cigarette smoke

Earth Marine Water and cigarette smoke

Earth Marine Water: 0.25% Incubation time: 1 hour Human fibroblast culture

Call viability (% of the control)

Protection against car exhaust fumes 110 100 90 80 70 60 50 40

Earth Marine Water protects by 78% the epidermis against car exhaust fumes

Control

Demineralized Earth Marine water and car water and car exhaust fumes exhaust fumes

Incubation time: 2 hour Reconstituted Human Epidermis

Protection against pollutants

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Epidermis in culture without car exhaust fumes (control).

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Epidermis with car exhaust fumes.

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Epidermis with Earth Marine Water and car exhaust fumes.

Anti-inflammatory effect

Inflammatory reaction induced by Keratinocyte will release IL1 Îą,

the main cause of redness, oedema and pain.

IL 1a (pg/ml)

14

Keratinocyte

12 10

Membrane phospholipid

8 6

IL1Îą

4

INFLAMMATION

2 0

Without UVb

With UVb (0.6J/cm2)

5% Earth Marine Water

25%

UVb + AntiInflammatory Cream

Redness

Oedema

Pain

Anti-allergenic effect

% inhibition of histamine release

Test on different intensity level of marine water to study Histamine (Mediator

of allergy). We found that Earth Marine water inhibits the histamine release

by substance P-stimulated Mastocytes.

100 90 80 70 60 50 40 30 20 10 0

Mastocytes Vesicles

1

3

4

10

Earth Marine Water (% v/v)

HISTAMINE Mediator of allergy Oedema Redness

Conclusion properties of marine spring water -

Stimulation of cell viability Improvement of epidermis cohesion Anti-free radical activity Protection against pollutants Anti-inflammatory effect

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BIO-Growth Factor

Placenta has been used as a major source of cosmetics in past few years due to Placenta is loaded with growth factor, protein, enzymes, and many elements more than 8,000 that are required for beauty. As a result of the limit of safety on genetic disease and difficulty of disease control, FDA has banned using Placenta in cosmetics.

Growth factor is necessary for cell renewal and removing old cells. With increasing age, growth factor production becomes less efficient and declines.

Normally, when growth factors start producing less after the age of 18 growth factors.

The Lost of Growth Factor

Fetus Fetus obtain all the nutrients and GF through placenta GF will stimulate the expansion of cells in the body system.

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0-3 years old Infants produce nutritional values and GF on their own.

10-13 years old GF production decreases and growth rate starts to slow down.

More than 18 years old GF production and the growth process decreases rapidly. Minimal amount is produced for metabolism.

GF decrease and absent of cell replacement leads to aging.

GF can be added to our skin for maintaining and rejuvenating youthful skin.

Innovation of supplying skin cells nutrients for maintaining youthful skin with safety

by using growth factors which made from genetically modification. We are done the production of growth factor in Escherichia coli through fermentation and extraction process, finally it becomes a purified growth factor, then we transformed it into nano liposome which skin can absorb effectively. Moreover,

it is more safety than using growth factor from human or animal which may consist

of contaminated germs or infected genes. http://mirworldwide.net

Bio-Placenta VS Human Placenta Human placenta

Effect

BIO-Placenta

Growth factor

EGF Skin rejuvenation EGF IGF-I Anti-wrinkle IGF-I Acidic FGF Anti-wrinkle Acidic FGF

Basic FGF Elasticity enhance Basic FGF

VEGF Nutritional supplement VEGF

TGF-β Anti-wrinkle - The minority parts - -

Vitamin Cell vitalization Vitamin:Vitamin B9 Essential Amino acids Energy supplement Amino acids: Acetyl Glutamine factor

Components of Bio-Placenta are similar to human placenta 1. Combination of 5 growth factors and essential factors make synergic

effect on skin - Stimulation of cell growth (Anti-wrinkle) - Anti-aging effect - Collagen biosynthesis Reduction of eye-wrinkle Before use

After use

Smoothness on the cheeks Before use

After use

Reduces wrinkles by 30 % in 4 weeks and smoothness on the cheeks by 21.7% http://mirworldwide.net

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2. Enhancing Wound Healing

Representative photographs highlighting how cell culture with Bio-placenta can accelerate wound closure. !

3. Stimulation of cell growth (Anti-wrinkle)

- Cell : Human Dermal Fibroblasts, Neonatal - Detection method : MTT assay Commercial Product BIO-Placenta

Growth race (%)

120 115 110 105 100 95 90 85 80

0

0.78125 1.5625 3.125 6.25 12.5 Concentration (mg/mL) Bio-Placenta is more effective than the commercial product in aspect of cell growth stimulation.

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Vitamin B9 or Folic Acid Vitamin B9 also called Folic Acid which is more popular than the word ‘vitamin B9’. People usually

do not know this type of vitamin

but they know Folic Acid instead.

It is one of the important vitamins;

it aids in the production of DNA

and RNA and also works closely

with vitamin B12 to help create

red blood cells.

The need of Folic Acid depends on a group of people, such as, pregnant women and anemia patient with high bleeding require more Folic because they tend to use high level of energy including high cell production.

Vitamin B9 or Folic Acid found in green vegetables, especially in spinach. FDA suggests that we should consume 400-600 mcg a day which is a significant element that helps stimulate protein and immune system production. Besides, it can help prevent heart attack and cancer.

Benefits of Folic Acid

1. Folic helps bone marrow to produce red blood cells.

2. It is the main element of Nucleic Acid production which is needed for growth and forming cell completely and properly.

3. Folic acid is crucial for proper brain function and plays an important role in mental and emotional health.

4. It associates with DNA and

RNA production. Folic Acid

is common found in green vegetables, moreover, in fresh fruits, pumpkin, peas, grains, avocado, whole wheat, rye bread, melon and cantaloupe.

Most Folic acid is in synthetic vitamins form but MIR brings innovative fermentation from fermented soybean to change structure of Folic Acid which provides more efficiency benefits

to your skin.

Helps reduce lines and wrinkles.

Reduces skin irritation.

Reduces Porphyrin which will

become toxic and cause the

inflamed acne when acne

bacteria exposes to UV ray.

Helps skin endure the UV

radiation.

Supports skin moisturizer.

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7 Products for sustainability of skin youthfulness at cellular level

MIR TRACE ELEMENTS MITONUTRIENTS FACE & EYE MAKE-UP REMOVER (For all skin type) MIR 9001 Price 120 RM

MIR TRACE ELEMENTS MITONUTRIENTS FACE WASH GEL (For all skin type) MIR 9002 Price 96 RM

MIR PV 20

Deep cleans all traces of makeup on entire face neck and around eye areas.

Leaves skin feeling fresh and clean.

Does not cause irritation.

Maintains skin moisture balance.

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Mild opulent formula for all skin types.

Removes leftover oil, make up and impurities from the pollution.

Leaves no dryness and irritation.

Maintains skin moisture balance.

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Innovation Trace Elements - Mitonutrients

MIR TRACE ELEMENTS MITONUTRIENTS DAY&NIGHT SERUM (For all skin type)

MIR TRACE ELEMENTS MITONUTRIENTS DAY CREAM SPF 60 PA+++ (For all skin type)

MIR 9003 9004 MIR Price 300 RM

Price 270 RM

Oil free formula for normal to oily and acne prone skin type.

Facial oil control.

Nourishes and keeps skin in well balance.

Promotes perfect skin with perfect cell repairing.

Helps to firm, tighten, smooth skin and diminish aging lines and wrinkles Slows down skin cell deterioration and extends youth looking skin.

MIR PV 55

60 times of UVA and UVB radiation protection longer than the natural skin protection .

Maintains skin cell’s moisture and well being balance.

Reinforces skin cell barrier.

Alleviates the sensation of redness, burn and uncomfortness due to sun exposure.

Stimulates cell repairing, helps to smooth and reduce appearance of wrinkles.

Slows down skin cell deterioration and extends the youth looking skin.

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Innovation Trace Elements - Mitonutrients

MIR TRACE ELEMENTS MITONUTRIENTS NIGHT CREAM (For all skin type) MIR 9005 Price 300 RM

MIR TRACE ELEMENTS MITONUTRIENTS EYE CREAM (For all skin type) MIR 9006 Price 450 RM

Designed for normal skin to dry skin type, supports skin cell’s natural moisture balance.

Promotes perfect skin with perfect cell reparing.

Delivers firmness and smoothness of skin, reduces signs of aging.

Slows down skin cell deterioration and extends the youth looking skin.

Helps to reduce the appearance of lines, wrinkles, puffiness, dark circle, sagginess and sac under eyes.

Provides the ultimate in hydration for visibly firm, uplift, supple, resilience, bright and youthful looking eyes.

Slows down skin cell deterioration and extends the youth looking skin.

Product application steps

Day Time (morning routine skin care regimen)

MIR 9002 Face Wash Gel

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MIR 9003 Day & Night Serum

MIR 9006 Eye Cream

MIR 9007 Neck Cream

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MIR 9004 Day Cream SPF 60 PA+++

Make-Up

for sustainability of skin youthfulness at cellular level

MIR TRACE ELEMENTS MITONUTRIENTS NECK CREAM (For all skin type) MIR 9007 Price 300 RM

Helps to firm, tighten and smooth away lines and wrinkles around the neck and decollete.

Reinforces Collagen and Elastin.

Prevents the quick loss of Elastin.

Slows down skin cell deterioration and extends the youth looking skin.

Product application steps

Night Time (night routine skin care regimen)

MIR 9005 Night Cream MIR 9006 Eye Cream

MIR 9002 MIR 9001 Make-up Remover Face Wash Gel MIR 9003 Day & Night Serum (oily & acne prone skin) http://mirworldwide.net

MIR 9007 Neck Cream

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MIR Trace Elements Mitronutrients

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