September 7, 2016 board packet

Regular Meeting of the Capitol Region Watershed District (CRWD) Board Of Managers, for Wednesday, September 7, 2016, 6:00 p.m. at the office of CRWD, 1410 Energy Park Drive, Suite 4, St. Paul, Minnesota.

REGULAR MEETING AGENDA I.

Call to Order of Regular Meeting (President Joe Collins) A) Attendance B) Review, Amendments and Approval of the Agenda

II.

Public Comment – For Items not on the Agenda (Please observe a limit of three minutes per person.)

III.

Permit Applications and Program Updates (Permit Process: 1) Staff Review/Recommendation, 2) Applicant Response, 3) Public Comment, and 4) Board Discussion and Action.)

A) Permit # 08-007 Earl-McLean Closeout (Hosch) B) Permit # 16-018 American Paper Building Demo ESC permit issued (Hosch) C) Permit # 16-021 2300 Territorial Rd. Apts. (Hosch) IV.

Special Reports –– No Special Reports

V.

Action Items A) AR: Approve Minutes of the August 17, 2016 Regular Meeting (Sylvander) B) AR: Adopt Preliminary 2017 Budget and Levy (Doneux) C) AR: Authorize 2017 Partner Grants Request of Proposals (Schwantes) D) AR: Authorize 2017 Special Grants Request for Proposals (Zwonitzer) E) AR: Award Bid for Willow Reserve Restoration Project (Zwonitzer) F) AR: Approve Monitoring Quality Assurance Program Plan (Vargo)

VI.

Unfinished Business A) FI: Crisis Communication Plan Update (Bromelkamp) B) FI: Snelling Midway Redevelopment Update (Eleria) C) FI: Central High School Stormwater Project Update (Zwonitzer)

VII.

General Information A) CAC Update

VIII. Next Meeting A) Wednesday, September 14, 2016 CAC Meeting B) Wednesday, September 21, 2016 Board Meeting IX.

Adjournment

W:\04 Board of Managers\Agendas\2016\September 7, 2016 Agenda.docx

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District

September 7, 2016 III. Permit Applications (A.) Permit Close Out (Hosch)

DATE: TO: FROM: RE:

August 25, 2016 CRWD Board of Managers Elizabeth Hosch Permit Closeout

Background Construction activity is complete for permit #08-007, Earl-McLean street reconstruction project.

Issues This permit was issued for the reconstruction of streets surrounding Earl and McLean in St. Paul’s Dayton’s Bluff neighborhood. The site spans the boundary between CRWD and the Ramsey Washington Metro Watershed District. Restrictive soil conditions resulted in 5 underground infiltration trenches being contained entirely in RWMWD. The RWMWD permit has been closed out and the trenches are inspected and maintained regularly. No surety was collected for the project.

Action Requested Approve Certificate of Completion for permit #08-007, Earl-McLean.

W:\07 Programs\Permitting\Board Memos\2016-09-07 Permit Closeout Board Memo.docx

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

Capitol Region Watershed District Applicant:

Mark Moorhouse Dominium Development 2905 Northwest Blvd, Suite 150 Plymouth, MN 55441

Permit 16-021 2300 Territorial Rd Apts Consultant: PJ Disch Loucks Associates 7200 Hemlock Lane, Suite 300 Maple Grove, MN 55369

Description: New apartment complex and associated infrastructure Stormwater Management: Two underground filtration systems District Rule: — C, F Disturbed Area: 2.45 Acres Impervious Area: 2.45 Acres STAFF RECOMMENDATION: Approve with 4 Conditions: 1. Documentation of maintenance agreement recorded with Ramsey County.

2. Provide plans signed by a professional engineer per the Minnesota Board of AELSLAGID. 3. Provide a copy of the NPDES permit.

4. Provide a site specific plan, schedule, and narrative for maintenance of the proposed stormwater management practices.

Permit Location Permit Report 16-021

Aerial Photo Board Meeting Date: 9-7-2016

Hampden Ave

Territorial Rd

Capitol Region Watershed District

Permit Report CRWD Permit #:

16-021

Review date:

August 30, 2016

Project Name:

Territorial Road Apartments

Applicant:

PJ Disch Loucks & Associates 365 Kellogg Boulevard East St Paul, MN 55101 763.496.6736

Purpose:

Building apartment complex and parking lot

Location:

2300 Territorial Road, St. Paul, MN 55114

Applicable Rules:

C, D, and F

Recommendation:

Approve with 4 Conditions

EXHIBITS: 1. Geotechnical Evaluation Report, by Braun Intertec, dated 6/2/16, rcvd. 7/29/16.

2. Stormwater Management Plan, by Loucks, dated 8/23/16, rcvd. 8/24/16.

3. Plan Set (Sheets C4-2, and C8-1), by Loucks, dated 8/29/16, rcvd. 8/29/16.

HISTORY & CONSIDERATIONS: None.

RULE C: STORMWATER MANAGEMENT Standards  Proposed discharge rates for the 2-, 10-, and 100-year events shall not exceed existing rates.  Developments and redevelopments must reduce runoff volumes in the amount equivalent to an inch of runoff from the impervious areas of the site.  Stormwater must be pretreated before discharging to infiltration areas to maintain the long-term viability of the infiltration area.  Developments and redevelopments must incorporate effective non-point source pollution reduction BMPs to achieve 90% total suspended solid removal.

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Findings 1. A hydrograph method based on sound hydrologic theory is used to analyze runoff for the design or analysis of flows and water levels.

2. Runoff rates for the proposed activity do not exceed existing runoff rates for the 2-, 10-, and 100-year critical storm events. Stormwater leaving the project area is discharged into a well-defined receiving channel or pipe and routed to a public drainage system.

3. Stormwater runoff volume retention is achieved onsite in the amount equivalent to the runoff generated from 1.1 inch of rainfall over the impervious surfaces of the development. a. The amount of proposed impervious onsite is 106,658 square feet. b. Volume retention required: 106,658 ft2 x 1.1 inches x 1 ft/12 inches = 9,777 ft3 Volume Retention Required (cu. ft.)

9,777

BMP

Total

Volume 1.1 inch Retention Runoff Provided below (cu. ft.) outlet (cu. ft.)

None. Filtration is proposed.

0 cf

2 inch Runoff (cu. ft.)

c. Filtration using sand media (55% credit) is proposed due to poor soils and contamination; filtration volume required: 9,777 ft3 x 1.82 credit factor = 17,794 ft3 Filtration Volume Required (cu. ft.)

17,794

BMP West Underground Filtration East Underground Filtration Total

Filtration Volume Provided below outlet (cu. ft.)

1.1 inch Runoff (cu. ft.)

2 inch Runoff (cu. ft.)

7,304

3,970

7,218

10,639

5,807

10,558

17,943 cf

d. Banking of excess volume retention is not proposed. e. Filtration volume and facility sizes have been calculated using the appropriate hydrological soil group classification and design infiltration rate. f. The filtration areas are capable of filtering the required volume within 48 hours. g. Stormwater runoff is pretreated to remove solids before discharging to filtration areas.

4. Alternative compliance sequencing has not been requested.

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5. Best management practices achieve 90% total suspended solids removal from the runoff on an annual basis.

6. A recordable executed maintenance agreement has not been submitted.

7. Adequate maintenance access is provided for surface and/or underground systems. A site specific plan, schedule, and narrative for maintenance of the proposed stormwater management practices has not been submitted.

RULE D: FLOOD CONTROL Standards  Compensatory storage shall be provided for fill placed within the 100-year floodplain.  All habitable buildings, roads, and parking structures on or adjacent to a project site shall comply with District freeboard requirements.

Findings 1. There is no floodplain on the property according to FEMA. 2. All habitable buildings, roads, and parking structures on or adjacent to the project site do comply with CRWD freeboard requirements.

RULE E: WETLAND MANAGEMENT Standard  Wetlands shall not be drained, filled (wholly or in part), excavated, or have sustaining hydrology impacted such that there will be a decrease in the inherent (existing) functions and values of the wetland.  A minimum buffer of 25 feet of permanent nonimpacted vegetative ground cover abutting and surrounding a wetland is required.

Findings 1. There are no known wetlands located on the property.

RULE F: EROSION AND SEDIMENT CONTROL Standards  A plan shall demonstrate that appropriate erosion and sediment control measures protect downstream water bodies from the effects of a landdisturbing activity.  Erosion Control Plans must adhere to the MPCA Protecting Water Quality in Urban Areas Manual.

Findings 1. Erosion and sediment control measures are consistent with best management practices, as demonstrated in the MPCA manual Protecting Water Quality in Urban Areas.

2. Adjacent properties are protected from sediment transport/deposition.

3. Wetlands, waterbodies and water conveyance systems are protected from erosion/sediment transport/deposition.

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4. Project site is greater than 1 acre; an NPDES permit is required. A SWPPP has been submitted and satisfies NPDES requirements.

RULE G: ILLICIT DISCHARGE AND CONNECTION Standard ďƒ˜ Stormwater management and utility plans shall indicate all existing and proposed connections from developed and undeveloped lands for all water that drains to the District MS4.

Findings 1. New direct connections or replacement of existing connections are not proposed.

2. Prohibited discharges are not proposed.

Recommendation: Approve with 4 Conditions Conditions: 1. Documentation of maintenance agreement recorded with Ramsey County.

2. Provide plans signed by a professional engineer per the Minnesota Board of AELSLAGID. 3. Provide a copy of the NPDES permit.

4. Provide a site specific plan, schedule, and narrative for maintenance of the proposed stormwater management practices.

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September 7, 2016 Board Meeting V. Action Item B) Approve Minutes of August 17, 2016 DRAFT Regular Board Meeting (Sylvander)

Regular Meeting of the Capitol Region Watershed District (CRWD) Board of Managers, for Wednesday, August 17, 2016 6:00 p.m. at the office of the CRWD, 1410 Energy Park Drive, Suite 4, St. Paul, Minnesota.

REGULAR MEETING MINUTES I.

A)

Call to Order of Regular Meeting (President Joe Collins)

Managers Joe Collins Seitu Jones Mary Texer Mike Thienes Shirley Reider

B)

Staff Present Jessica Bromelkamp, CRWD Mark Doneux, CRWD Anna Eleria, CRWD Forrest Kelley, CRWD Michelle Sylvander, CRWD

Public Attendees Carrie Wasley, RCD Scott Spaeth, Roselawn Cemetery Larry Hudella, Roselawn Cemetery

Review, Amendments and Approval of the Agenda

President Joe Collins opened the meeting at 6:00 p.m. and asked for any additions or changes to the agenda. Administrator Doneux requested the addition of VI. D. Building Update.

Motion 16-201: Approve the August 17, 2016 as amended with the addition of VI. D. Building Update.

Reider/Texer Unanimously approved II.

Public Comment

Ms. Wasley shared with the Board of Managers that the Ramsey Conservation District will be having their annual tour at the Frog Town Farms on September 7th from 1:00-3:00.

III.

Permit Applications and Program Updates A)

15-040 Luther Seminary 60-day Renewal Request (Kelley)

Mr. Kelley reviewed that the applicant has requested the review period be extended due to insufficient soil information.

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District

Motion 16-202: Approve the 60-day review period extension for permit 16-040 Luther Seminary to expire October 27, 2016.

Reider/Texer Unanimously approved B)

16-019 Roselawn Cemetery (Kelley)

Mr. Kelley reviewed permit #16-019 Roselawn Cemetery. The applicant, Roselawn Cemetery, plan to create a new water feature. The applicable rules are Stormwater Management (Rule C), Flood Control (Rule D) and Erosion and Sediment Control (Rule F). The disturbed area of this project is 6.9 acre with 0.9 acres impervious surface.

Motion 16-203: Approve permit 16-019 Roselawn Cemetery with 3 conditions: 1. Receipt of $4,500 surety and documentation of maintenance agreement recorded with Ramsey County.

2. Provide a site specific plan, schedule and narrative for maintenance of the proposed stormwater management practices that includes the following: a. Establish a watering plan that extends a minimum of one year after planting. b. At a minimum, require annual maintenance of infiltration areas to include trimming vegetation, replacing vegetation where needed, mulch replacement, and removal of accumulated sediment and debris.

3. Revise the cross-section detail of the rock swale connecting Pond 1 and Pond 2. The detail shows the entrance from Pond 1 at 967.0 Sheet C3-1 and the HydroCAD show the emergency overflow at 964.0.

Thienes/Reider Unanimously approved PUBLIC HEARING – WATERSHED DISTRICT 2017 Budget and Levy President Collins opened the Public Hearing and requested that communication of the public hearing be included in the record. The proposed 2017 budget was reviewed by the Citizen Advisory Committee on August 10, 2016. Notice of the Public Hearing was posted in the Pioneer Press. The Draft Budget and Levy was also distributed to our Cities. Information of the Public Hearing and proposed budget was also posted on CRWD’s website. President Collins attended the CAC meeting on August 10th. Administrator Doneux reviewed with the draft 2017 Budget for the Board of Managers. Manager Jones asked to see more detailed graphs of the tax impacts be posted on the website. Administrator Doneux asked for any changes or comments to the draft budget. Manager Thienes commented that any changes could be made in December before the final approval on December 21st.

There were no public attendees for the public hearing.

Motion 16-204: Close the Public Hearing for the Proposed 2017 Budget and Levy of the Capitol Region Watershed District.

Reider/Texer Unanimously approved

IV.

Special Reports – No Special Reports

V.

Action Items A)

AR:

Approve Minutes of the August 2, 2016 Special Meeting (Sylvander)

Motion 16-205: Approve Minutes of the August 2, 2016 Special Meeting.

Jones/Reider Unanimously approved B)

AR:

Approve Minutes of the August 3, 2016 Regular Meeting (Sylvander)

Motion 16-206: Approve Minutes of the August 3, 2016 Regular Meeting.

Jones/Reider Unanimously approved C)

AR:

Approve Accounts Payable/Receivables for July 2016 (Sylvander)

Motion 16-207: Approve July 2016 Accounts Payable/Receivable and July Budget Report, direct Treasurer and Board President to endorse and disperse checks for these payments.

Thienes/Reider Unanimously approved D)

AR: Award Bid for Trout Brook Stormwater Interceptor Repairs Sta. 0+00 to 28+49 (Eleria)

Ms. Eleria reviewed that in June, CRWD’s Board of Managers authorized solicitation of bids for the TBI Repair Project from Station 0+00 to 28+49. The repair project will address the missing mortar between the limestone blocks as well as other defects in the tunnel. In the first 2,000 feet of TBI, the gaps between the limestone blocks will be filled in with mortar. A 12-inch thick reinforced concrete liner will be installed in 200 feet of TBI located beneath the railroad tracks. For the last 300 feet of TBI, mortar replacement or 2-inch thick concrete liner repairs were being considered. The engineer’s estimate for construction costs ranged from $1.1 – $1.3 million.

Bidding for the project extended over a three-week period from July 12 to August 4, 2016. CRWD received base bids from three contractors: 1) PCI Roads - $1,679,300; 2) Lametti & Sons, Inc. $2,100,750; and 3) ECI - $1,195,375. CRWD also sought quotes for an alternative repair for the last 300 feet of TBI - 2-inch thick concrete liner in lieu of mortar repair. ECI’s estimated cost of the

alternative repair is $25,430. The mortar repair will restore the original integrity of the TBI joints while the liner repair may improve the structural integrity of this section of TBI. Based on the lowest bid from ECI and their successful tunnel repair projects in the Twin Cities, CRWD staff recommends the Board award the project to ECI and select the bid alternate item (2-inch concrete liner) for the last 300 feet of TBI. The total bid amount is $1,220,805.

Ms. Eleria shared with the Managers that the anticipated start date would be December 1st with most of the work being done in 2017. Manager Jones asked if a time lapse video would be made. Ms. Eleria replied that she would look into it.

Motion 16-208: Authorize the Board President and Administrator to execute a Notice of Award and an Agreement with ECI for the Trout Brook Repair Project Station 0+00 to 28+49 subject to the review and approval of the Ramsey County Attorney; and authorize the Administrator to execute change orders in an amount not to exceed $61,000.

Reider/Texer Unanimously approved E)

AR:

Adopt 2016 CRWD Award Program (Bromelkamp)

Ms. Bromelkamp reviewed that in 2014, the Citizen Advisory Committee (CAC) adopted the CRWD Watershed Steward Award Program to recognize residents and partners of the District for exemplary stewardship activities and projects. The committee developed five award categories including the Watershed Citizen, Watershed Partner, Young Watershed Steward, Watershed Project, and Lifetime Stewardship Awards. The program was promoted by CRWD staff, Board and CAC members.

Ms. Bromelkamp reviewed a proposed timeline for review and comment.

Motion 16-209: Approve the 2016 CRWD Watershed Steward Award program and authorize staff to solicit nominations.

Reider/Texer Unanimously approved F)

AR: Approve Work Plan and Agreement with Houston Engineering for Phase II Como Park Stormwater Management Plan (Zwonitzer)

Mr. Zwonitzer reviewed with the Managers that in September 2014, the CRWD Board approved a Memorandum of Understanding (MOU) with the City of St. Paul Parks and Public Works Departments to develop a stormwater management master plan for Como Regional Park. The MOU outlined shared goals of intentional management of stormwater as a resource, and protection of the water resources within Como Park. Subjects that must be addressed include an inventory of existing conditions, and development of a 10-20 year stormwater plan. In January 2016, the stormwater inventory was completed by Houston Engineering. CRWD and the City are now prepared to begin developing the stormwater plan.

A stormwater master plan for Como Park will result in identifying cost-effective BMP implementation strategies within the park. The plan will assess future treatment needs within the park based on planned projects and City/CRWD rules, and establish goals for stormwater treatment that achieve TMDL load reduction requirements. The plan will also Priority areas for treatment within the park will be established, and potential project sequencing scenarios will be analyzed to assist with implementation planning. Because sequencing of park improvements and BMPs to treat them could present regulatory challenges, the plan will develop policies that addresses stormwater treatment requirements and the need for strategic project implementation.

Houston Engineering has prepared a proposal for developing the stormwater master plan, which is estimated to cost $47,110. CRWD and City staff have reviewed this proposal and recommend approval. The 2016 budget for this project is $49,240 from fund #305 – Como Lake Subwatershed.

Motion 16-210: Approve consultant services agreement with Houston Engineering for the Como Regional Park Stormwater Management Plan for an amount not to exceed $47,110, and authorize the Administrator to execute the agreement and change orders not to exceed $5,000.

Reider/Thienes Unanimously approved G)

AR: Approve License Agreement with City of Saint Paul for Willow Reserve Restoration Project (Zwonitzer)

Mr. Zwonitzer reviewed the Willow Reserve Ecological Restoration Project. This project involves work on property owned by City of Saint Paul’s Parks and Recreation and Public Works departments.

Using the Highland Ravine Project license agreement with the City, CRWD staff have prepared a draft license agreement for access and implementation of the Willow Reserve Ecological Restoration Project on City parkland. The agreement has also been reviewed by the Ramsey County Attorney. The agreement grants CRWD the rights to access the property to manage invasive species, remove undesirable trees, and restore native vegetation as outlined in the Willow Reserve Restoration Plan. It allows for restoration activities to occur through the end of 2019. The draft agreement has been sent to the City for review.

Motion 16-211: Approve license agreement with the City of Saint Paul for the Willow Reserve Ecological Restoration Project subject to approval of the Ramsey County Attorney.

Reider/Thienes Unanimously approved VI.

Unfinished Business A) FI: Snelling Midway Redevelopment Update (Eleria)

Ms. Eleria shared with the managers that she attended the City of St. Paul public hearing. CRWD provided testimony and updates to the City Council members on August 3rd. Administrator Doneux

shared with the managers that a letter was sent to the property owners. CRWD was able to meet with the property owners and share concerns about a district wide approach for stormwater. Manager Texer asked what passed at the public hearing. Ms. Eleria replied that the City Council approved the Master Plan for the stadium.

B) FI: Willow Reserve Update (Eleria) Update was already provided by Mr. Zwonitzer with the license agreement under Action Item V. G. C) FI: Central High School Stormwater Project Update (Zwonitzer) Mr. Zwonitzer provided the managers with a review of the project and shared the site plan. Contractors have had a difficult time working in the confined spaces available. Final touches are just getting wrapped up. A ribbon cutting ceremony is planned for Tuesday, September 6th from 8:30-9:30. Governor Dayton, Mayor Coleman and other guests are anticipated to attend.

D) FI: Building Update (Doneux) Administrator Doneux provided the managers with an update on the due diligence process. Barr Engineering will be reviewing phase one of the environmental site assessment land. Administrator Doneux will be getting a quote to provide a property condition assessment. The property condition assessment will include an inspection of the roof, HVAC, walls, plumbing and mechanical systems.

President Collins asked if all the building structures will be included in the assessment. Administrator Doneux replied that we will be conducting the assessment at all three buildings. Two underground storage tanks were removed in the 1980’s and additional environmental testing will be taking place. A site walk thru is scheduled for next Thursday to help with the due diligence process. Manager Thienes plans to attend. Manager Texer asked when the current property owners will be moving out. Administrator Doneux thought they plan on moving out in October. A lease back provision is included in the purchase agreement.

VII.

General Information

A) CAC Update President Collins attended the August 10th, 2016 CAC meeting. Ms. Schwantes provided the committee with an update on the Master Water Steward Program. Administrator Doneux reviewed the 2017 draft budget.

VIII. Next Meetings A) B) C) D) E) F)

Monday, August 22, 2016, Willow Reserve Wednesday, August 31, 2016 Crisis Communication Training Tuesday, September 6, 2016, Master Water Stewards Bang Brewing Wednesday, September 7, 2016 Board Meeting Wednesday, September 14, 2016, CAC Meeting – Manager Reider will attend Wednesday, September 21, 2016 – Manager Jones will be absent

Our mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

IX.

Adjournment

Motion 16-212: Adjournment of the August 17, 2016, Regular Board Meeting at 7:29 p.m.

Reider/Texer Unanimously Approved Respectfully submitted, Michelle Sylvander W:\04 Board of Managers\Minutes\2016\August 17, 2016 Draft.docx

Our mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

September 7, 2016 Board Meeting V. Action Item B) Approve Preliminary 2017 Budget and Levy (Doneux)

DATE: TO: FROM: RE:

September 1, 2016 CRWD Board of Managers Mark Doneux, Administrator Adopt Preliminary 2017 Budget and Certification of Levy

Background The Board has discussed and reviewed the preliminary 2017 Budget and Tax Levy during meetings and workshops this summer. A copy of our summary budget and hearing notice was sent to the affected cities and Ramsey County officials in August. The Board of Managers held a special workshop on August 2, 2016 to review the draft budget and levy. The Citizen’s Advisory Committee reviewed and recommended approval on the proposed 2017 budget and levy at their August meeting. A duly noticed Public Hearing was held on August 17th to collect public comment on the proposed 2017 budget and levy. The Board of Managers must adopt a Preliminary 2017 Budget and Levy Certification before the initial September 15, 2015 deadline.

Issues I am recommending that the Board adopt the preliminary budget and levy as presented. While we have submitted our preliminary draft budget to our cities we have not received any comments as of September 1st. If the Board intends to reduce the budget or levy, I would recommend doing that at the December, Final Levy Certification to allow for better estimating of fund balance as well as external budget requests.

Since the Public Review Draft was distributed, revisions to the draft budget presented total $81,594. The proposed tax levy remains unchanged. Staff will review the minor changes to the buget.

Staff is recommending the preliminary budget of $332,420 for Administration, $2,062,432 for Programs, $782,378 for Projects for a total Operations Budget of $3,177,230 and $9,064,442 for Capital Improvement Projects. This results in a total Preliminary 2017 Budget of $12,241,672. This is an increase of $3,417,241 from 2016. The proposed 2017 tax levy is $4,915,672. This includes $4,546,754 for the operations and capital levy and $368,918 for debt. This levy is $647,901 or 15.18% higher than 2016.

Requested Action Approve and adopt the Preliminary 2017 Budget and Levy Certification. enc:

Property Tax Impacts (graphic) Preliminary 2017 Project List (11”x17”) Preliminary 2017 CRWD Expenditure, Revenue and Levy Summary (11”x17”) Draft Levy Certification form Draft Debt Levy Certification form Draft Resolution – Adopting 2017 Budget & Levy

W:\02 Budget and Finance\Budget - 2017\Preliminary Certification\Board Memo - Adoption of Preliminary 2016 Budget and Levy - 9-1-16.docx

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

September 7, 2016 V. Action Item C. Authorize 2017 Partner Grant RFP (Schwantes)

DATE: TO: FROM: RE:

September 1, 2016 CRWD Board of Managers Lindsay Schwantes Authorize Distribution of 2017 Partner Grant Request for Proposals

Background CRWD seeks to expand its reach and increase the number and diversity of partners through its Partner Grants program. Partner Grants are made to organizations, schools, businesses and other entities and range from $2,000 to $20,000 for clean water education and outreach projects.

Issues Staff will distribute the grant solicitation for 2017 Partner Grants to the attached list and any additional community organizations, cities or agencies suggested by the Board of Managers. The distribution list includes previous grant recipients and additional organizations that represent new audiences for CRWD. The 2017 budget for Partner Grants is $90,000.

2017 Partner Grants Timeline: Task Date Board authorized RFP for 2017 Partner Grants and identifies two September 7, 2016 representatives for Partner Grant Committee September 8, 2016 September 14, 2016 October 31, 2016 November 2016 December 7, 2016 December, 2016

RFP for 2017 Partner Grants proposals distributed CAC identifies one representative for Partner Grant Committee Proposals due Joint-committee makes funding recommendations for Board approval Board approves joint-committee recommendations for winners 2017 grant recipients notified and agreements sent

Staff request that the Board establish a 2017 Partner Grants Committee and select two Board members to serve on the committee and will request that CAC select a member to serve on the 2017 Partner Grants Committee.

Request Action Authorize staff to distribute CRWD 2017 Partner Grant Request for Proposals and proceed with the grant approval process according to the proposed timeline.

Enc:

2017 CRWD Partner Grant Request for Proposal and Application 2017 CRWD Partner Grant Distribution List

W:\07 Programs\Stewardship Grant Program\Partner Grants\2017 Grants\BM 2017 Partner Grant RFP.docx

2017 Partner Grant Request for Proposals September 8, 2016 Dear Community Partner, CRWD is requesting proposals for 2017 Partner Grants now through Monday, October 31, 2016 at 4pm.

CRWD Partner Grants support organizations who share in its mission to protect, manage and improve water resources. Eligible clean water projects may include education programs and materials, projects that involve community organizing, training, events or clean water landscaping projects.

All grantees are required to track and report general information about project participation. Priority will be given to applicants who propose to also measure project impacts and benefits. This may include pollution reduction, the measurement of resident actions taken, commitments to clean water actions made. Projects that receive grant funding must be competed and a final report submitted to CRWD by December 31, 2017.

In 2017, $90,000 is available for grants ranging from $2,000 to $20,000. Groups within CRWD eligible to receive a grant include nonprofit organizations, Saint Paul District Planning Councils, neighborhood groups, schools, local government units, businesses and faith groups. Grants are made on a reimbursement basis.

2016 Partner Grants Timeline: October 31, 2016 November 2016 December 7, 2016 December, 2016

Proposals due by 4:00pm Joint-committee makes funding recommendations for Board approval Board approves joint-committee recommendations for winners 2017 grant recipients notified and agreements sent

An application is attached in both Word and .PDF formats, or you may apply online at capitolregionwd.org/our-work/grants. Application materials and a list of past recipients can be found on our website.

Contact Lindsay with any questions. lindsay@capitolregionwd.org or (651) 644-8888.

W:\07 Programs\Stewardship Grant Program\Partner Grants\2017 Grants\2017 Partner Grant announcement letter.docx

Our mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

Capitol Region Watershed District

1410 Energy Park Drive, Suite 4 Saint Paul, MN 55108 Capitolregionwd.org (651) 644-8888

PARTNER GRANT APPLICATION FORM Please submit form and required materials to: LINDSAY SCHWANTES lindsay@capitolregionwd.org

PROJECT INFORMATION PROJECT NAME

APPLICANT INFO

APPLICANT INFORMATION ORGANIZATION OR AGENCY

CONTACT PERSON

ZIP

CITY

ADDRESS

PHONE

EMAIL ADDRESS

WHAT GEOGRAPHIC AND/OR DEMOGRAPHIC AREA DO YOU SERVE?

HOW MUCH ARE YOU REQUESTING? (BETWEEN $2,000 AND $20,000)

HOW DID YOU HEAR ABOUT OUR GRANTS?

PROJECT DESCRIPTION

PROJECT BACKGROUND

1.) DESCRIBE THE MISSION AND GOALS OF YOUR ORGANIZATION AND HOW YOUR PROJECT HELPS MEET THEM

1

2.) DESCRIBE HOW YOUR PROJECT WILL PROTECT OR IMPROVE WATER QUALITY.

PROJECT BACKGROUND

3.) IF APPLICABLE, PLEASE LIST OTHER PARTNERS AND THIER ROLE IN YOUR PROJECT.

MEASUREMENT AND EVALUATION 4. ) BRIEFLY DESCRIBE HOW WILL YOU MEASURE THE SUCCESS OF YOUR PROJECT?

2

PROJEC TASKS 5.) IN THE SPACE BELOW, PLEASE BREAK DOWN YOUR PROJECT INTO MAJOR TASKS (UP TO 5), BRIEFLY DESCRIBE THEM IN THE SPACE PROVIDED AND ESTIMATE COMPLETION DATE FOR EACH.

1

TASK NAME:

ESTIMATED COMPLETION DATE (M/Y):

DESCRIPTION:

2

TASK NAME:

ESTIMATED COMPLETION DATE (M/Y):

PROJECT DESCRIPTION

DESCRIPTION:

3

TASK NAME:

ESTIMATED COMPLETION DATE (M/Y):

DESCRIPTION:

4

TASK NAME:

ESTIMATED COMPLETION DATE (M/Y):

DESCRIPTION:

5

TASK NAME:

ESTIMATED COMPLETION DATE (M/Y):

DESCRIPTION:

3

PROJECT BUDGET COMPLETE THE FOLLOWING TABLE FOR ALL PROJECT TASKS FOR WHICH YOU WILL BE REQUESTING GRANT FUNDS. NOTE: MATCH FUNDS ARE NOT REQUIRED, BUT PREFERENCE MAY BE GIVEN TO PROJECTS WITH DEDICATED FUNDS. MATCH FUNDS MUST BE CASH (IN-KIND LABOR NOT CONSIDERED).

TASKS

PERSONNEL / STAFF COSTS

OTHER EXPENSES (MATERIALS / SUPPLIES)

TOTAL COST

TASK 1:

$ 0.00 TASK 2:

$ 0.00

TASK 3:

$ 0.00 TASK 4:

$ 0.00 TASK 5:

$ 0.00

PROJECT FINANCIALS

TOTALS

$ 0.00

$ 0.00

$ 0.00

GRANT FUNDING PLEASE FILL IN THE TABLE BELOW WITH HOW YOU PLAN TO ALLOCATE YOUR FUNDING. NOTE: THE TOTAL COST FROM THE TABLE ABOVE AND THE TOTAL FUDNING BELOW SHOULD BE THE SAME.

TASK

REQUESTED CRWD FUNDING

FUNDING FROM OTHER SOURCE

TOTAL

TASK 1:

$ 0.00 TASK 2:

$ 0.00 TASK 3:

$ 0.00 TASK 4:

$ 0.00 TASK 5:

$ 0.00 TOTALS

$ 0.00

$ 0.00

$ 0.00

4

2017 CRWD Partner Grant Promotion List Type of group Art/Community

Organization

Name

Wisdom Dances Hark Designs Public Art Saint Paul Public Art Saint Paul U of MN COMPAS Hmongtown Marketplace - Frogtown Green Artist Healther Cole Gita Ghei Lisa Elias Brad Kaspari

Emily Jarrett-Hughes Mary Hark Mary Johnson Colleen Sheehy Christine Baeumler Betsy Mowry Voss

City of Falcon Heights City of Falcon Heights City of Roseville City of Roseville City of Lauderdale City of Lauderdale City of Maplewood Saint Paul Natural Resources Saint Paul Parks and Recreation Saint Paul Parks and Recreation Saint Paul Saint Paul Saint Paul Saint Paul Saint Paul Saint Paul Saint Paul Saint Paul

Beth Mercer-Taylor Sack Thongvanh Ryan Johnson Lonnie Brokke all city council and staff Heather Butkowski Chris Swanson Faith Krogstad Mary Henke-Haney Adam Robbins Margaret Jones Alice Messer Kathleen Anglo Barb Mundahl Cy Kosel Kris Hagemann Matt Morreim Wes Saunders-Pearce

Ramsey Conservation District Ramsey Conservation District Ramsey County Commissioners Ramsey County Commissioners Ramsey County Commissioners

Anne WhiteEagle Joe Lochner Janice Rettman Toni Carter Rafael Ortega

Ward 1

Dai Thao Mai Chong Xiong Rebecca Noecker Taina Maki Chris Tolbert Libby Kantner Russ Stark Samantha Henningson Amy Brendmoen Kim O'Brien Dan Bostrom Scott Renstrom Jane Prince Stephanie Harr

Title

Sieng Lee Heather Cole Gita Ghei Lisa Elias Brad Kaspari

City Council Member City Administrator Environmental Specialist Parks & Recration Director City Administrator Environmental Technician Education Coordinator Environmental Coordinator 8-80 Fellow PED Saint Paul Parks and Rec Project Manger

Water Resource Coordinator

County

District 3 District 4 District 5

Saint Paul City Council

Ward 2 Ward 3 Ward 4 Ward 5 Ward 6 Ward 7

Legislative Aide Legislative Aide Legislative Aide Legislative Aide Legislative Aide Legislative Aide Legislative Aide

District Planning Councils District 1 District 16 District 16 District 4 - Dayton's Bluff

Betsy Leach Nelima Sitati Theresa Nix Deanna Abbott-Foster

Community Organizer Executive Director Community Organizer Executive Director

District 10 -Como Community Council Michael Kuchta

Executive Director

District 11 -Hamline Midway Coalition Michael Jon Olson

Executive Director

District 11 -Hamline Midway Coalition District 12 -St. Anthony Park District 12 -St. Anthony Park District 13 -Union Park District 13 --Union Park District 14 -Macalester Groveland District 15 -Highland District 15 -Highland District 3 -West Side Citizens Organization District 4 -Dayton's Bluff District 5 -Payne Phalen District 5 -Payne Phalen District 6 -North End/South Como District 7 -Frogtown Neighborhood Association District 7 -Frogtown Neighborhood Association District 8 -Summit-University District 8 -Summit-University District 9 -West 7th and Fort Road Federation District 9 -West 7th and Fort Road Federation District 17 -Capitol River Railroad Island

Melissa Cortes Suyapa Miranda Cailin Rogers Julie Reiter Michael Johnson Liz Boyer Kathy Carruth

Communications Coordinator Executive Director Outreach and Operations Coordinator Executive Director Community Organizer Executive Director Executive Director

Monica Bravo Nicole Pressley Lissa Jones Lofgren Robin Horkey Kerry Antrim

Community Organizer Communications Manager Interim Executive Director Outreach & Administrative Assistant Executive Director

Caty Royce

Director

Sam Buffington Sara Udvig Roxanne Draughn

Community Organizer Executive Director Community Organizer - Youth Development

Ed Johnson

Executive Director

Betty Moran Paul Bengtson Don Lorr

Community Organizer Director

BOMA Fortin Consulting Saint Paul Chamber of Commerce

Kevin Lewis Connie Fortin Jonathan Weinhagen

Executive Director

Commercial Property Management

Vice President

Community Citizens League/Como Active Citizens Network District Councils Collaborative Lake McCarrons Neighborhood Association Lake McCarrons Neighborhood Association Lower Phalen Creek Project Model Cities Model Cities Como Lake Neighbors / MN Dragonfly Society Rondo Ave. Inc

Janna Caywood Carol Swenson

Executive Director

Sherry Sanders Rick and Meg Cavalier Beverly Hawkins Craig Johnson Jacki Morrison

CEO Real Estate Development Project Manager

Au

Aurora St Anthony Development Corporation Frogtown Farm Frogtown Farm Urban Boat Builders Urban Boat Builders Freshwater Society University of Minnesota Extension Saint Paul Riverfront Corporation Frogtown Railroad Island Gloria Dei Lutheran Church Macalester Plymouth Presbytarian Saint Paul Recreation Centers Creative Enterprise Zone

Nieeta Presley Eartha Bell Stephanie Hankerson Angela Robins Marc Hosmer Jen Kadar Sam Bauer Tim Griffin Melvin Giles Don Lorr Meg Stevenson Mark Dickinson Gwen Peterson Amy Sparks

ED ED Community Organizer Program Manager and Instructor Executive Director Program Manager U of M Extension Educator Director of Urban Design

Va-Megn Thoj

Executive Director

Oksar Ly

Artist Organizer

Joo-Hee Pomplun

Director of Programs

Jeffrey Whitman

Event Manager

Teeko Yang Jamie Liu Toua Xiong Pao Yang Yer Xiong Cheng Lee Felipe Espinoza-Day Alexis Walstad Eh Tah Khu Nancy Her Mohamed Farah Chong Lee

Project Associate Manager Hmongtown Marketplace owner Hmongtown Marketplace

Sharon Day

Executive Director

Recreation Programming Manager

Cultural Asian Economic Development Association Asian Economic Development Association Asian Economic Development Association Asian Economic Development Association Asian Economic Development Association Hmongtown Marketplace Asian Outdoor Heritage Asian Outdoor Heritage Asian Outdoor Heritage Ce Tempoxcalli Karen Organization of MN Karen Organization of MN Hmong Corps Ka Joog Lao Family Community Lao Family Community Lao Family English School Indigenous Peoples Task Force African Economic Development Solutions Environmental Alliance for Sustainability Friends of the Mississippi River Friends of the Mississippi River Frogtown Green Full Spring Studio Great River Greening Urban Roots Watershed Partners Ramsey County Master Gardeners Mississippi River Fund Mississippi Park Connection Trust for Public Land Wilderness Inquiry Wilderness Inquiry

Sean Gosiewski Irene Jones Trevor Russell Patricia Ohmans Jonee Kulman-Brigham Deborah Karasov Patsy Noble Jana Larson Jan Morlock Katie Nyberg Mary Hames Jenna Fletcher Meg Krueger Gwen Wilson

Co-Executive Director Co-Executive Director Executive Director Executive Director

Saint Paul Audubon Saint Paul Audubon Wild Ones - Big River Big Woods Heather Holm

Karen Eckman Howard Markus Eva Ekola Heather Holm

MISC. Hamline Midway Neighborhood Contact MN SUP Como Neighborhood Como Neighborhood Master Water Steward Master Water Steward Master Water Steward Master Water Steward Master Water Steward Master Water Steward Midway Murals Hamline Midway Resident

Erin Pavlica Dave Englund Barb Spears Sharon Shinomaya Val Cunningham Dave Pasiuk Lucy Hunt Sarah Olson Amy Delbecq Lauren Colwell Alero Moju Jonathan Oppenheimer Lily Shaw

Cretin-Derham Hall Cretin-Derham Hall

Steven Tacheny Julie Quinn-Kiernan

Green Team Advisor Green Team Advisor

Twin Cities German Immersion School Central High School Central High School City Academy Transforming Central Great River School Great River School Harambee Elementary

Jeff Horton Mary Mackbee Lisa Houdek Mem Lloyd Julie Marckel Melanie Peterson-Nafzinger Scott Alsleben Jenny Eckman

Assistant Executive Director Principal Science Faculty Teacher Parent leader History Guide and IB Coordinator Field Studies Environmental Science Specialist

Kitty Andersen Youth Science Center

Shona Ramchandani

Saint Paul Academy Saint Paul Public Schools Saint Paul Public Schools Falcon Heights Elementary Friends School of Minnesota Brimhall Elementary Parkview Parkview Roseville Earth Club

Chris Collins Samantha McKeough Lisa van der Steur Falcon Heights Elementary Elementary Penny Bidne Kristen Smith Olson Mary Sweeney Pierre MacGillis

High School Program Manager Middle School Faculty: Math, Science, Technology Environmental Service Assistant Senior Development Specialist Principal Science Teacher Principal Principal teacher Earth Club Advisory

Macalester College Macalester College

Christine Manning Roopali Phadke

Macalester College

Dan Hornbach

Hamline University Concordia College University of Minnesota University of Minnesota University of Minnesota University of Minnesota U of MN Extension

Ken Dehkes Leanne Bakke Kristen Nelson Sarah Hobbie Vanessa Perry Mae Davenport John Bilotta

School

Assistant Professor in Environmental Studies Professor or Environmental Studies Chair of Environmental Studies - Aquatic ecology and conservation Director of Facilities Operations and Horticultural Services Chair of Biology

Stormwater Education Program Educator

University of Minnesota Water Resources Center University of Minnesota Water Resources Center University of Minnesota Institute on the Environment

Faye Sleeper

Associate Director

Ann Lewandowski

Research and Outreach Coordinator

Jan Gerstenberger

Director of Partnerships and Development

Youth Boys Scouts of MN-Northstar chapter Joe Jansen

District Chair

Boys Scouts of MN-Northstar chapter Padriac McGuire

District Commissioner

Boys Scouts of MN-Northstar chapter Jordan Waggoner Girl Scouts River Valley Celina Martina

District Executive Director of Community Partnerships

September 7, 2016 V. Action Items D) Authorize 2017 Special Grant RFP (Zwonitzer)

DATE: TO: FROM: RE:

August 29, 2016 CRWD Board of Managers Nate Zwonitzer, Water Resource Project Manager Authorize 2017 Special Grant Request for Proposals

Background Each year CRWD allocates funds for budget 440-Special Projects and Grants. The goal of this program is to partner with government units and other entities (public and private) to cooperatively implement capital improvement projects for water resource protection and improvement.

Issues Staff will distribute the grant solicitation for 2017 Special Grants to the attached list and any additional organizations suggested by the Board of Managers. The 2017 budget for Special Grants is $350,000.

2017 Special Grants Timeline: Task Date Board authorized RFP for 2017 Special Grants and identifies two September 7, 2016 representatives for Special Grant review committee September 8, 2016 September 14, 2016 October 31, 2016 November 2016 December 7, 2016 December 2016

RFP for 2017 Special Grants RFP distributed CAC identifies one representative for Special Grant Review Committee Grant proposals due Joint-committee makes funding recommendations for Board approval Board approves grant awards 2017 grant recipients notified – begin developing grant agreements

Staff request that the Board establish a 2017 Special Grant Review Committee and select two Board members to serve and will request that the CRWD CAC select one member to serve on the 2017 Special Grant Committee.

Action Requested Authorize distribution of the 2017 Special Grants Request for Proposals to District partners.

enc:

2017 Special Grant RFP and application 2017 Special Grants Solicitation Distribution List

\\CRWDC01\Company\06 Projects\Special Grants\2017\Bd Memo 2017 Special Grants 09-07-2016.docx

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

September 8, 2016 To: Government units and other entities within Capitol Region Watershed District RE: 2017 SPECIAL PROJECT AND GRANTS PROGRAM Dear potential project partner: Capitol Region Watershed District (CRWD) is now accepting applications for our 2017 Special Grant program. The District has established this program to partner with government units and other entities to cooperatively implement capital improvement projects for water resource protection and improvement.

CRWD aims to accomplish one or more of the following goals working with Special Grant program partners:     

Improve water quality by decreasing stormwater runoff and increasing pollutant removal Preserve and protect plant and wildlife communities adjacent to lakes, rivers and wetlands Establish links between people and water resources through education Provide demonstration projects for innovative best management practices Support projects that improve water quality and that serve as an asset and resource for the public and local community.

The District encourages you to review the enclosed Special Grant Program description and example application form. An electronic version of the application is available at www.capitolregionwd.org.

Applications must be submitted by October 31, 2016. Please feel free to contact me with any questions or to discuss a potential project.

Sincerely,

Nate Zwonitzer Water Resource Project Manager nate@capitolregionwd.org 651-644-8888 enc:

Special Project and Grant Program, 2017

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

Special Project and Grant Program, 2017 Capitol Region Watershed District’s (CRWD) mission is to protect, manage and improve the water resources of the District. The District has established a Special Grant Program to partner with government units and other entities to cooperatively implement capital improvement projects that benefit our water resources. Grant applications are due to CRWD by 4pm on Monday, October 31, 2016.

GOALS The Special Grant program aims to accomplish one or more of the following goals:  Improve water quality by decreasing stormwater runoff and pollutants (treating 1 inch of runoff from impervious surfaces is a good goal for grant projects)  Preserve and protect plant and wildlife communities adjacent to lakes, rivers and wetlands  Establish links between people and water resources through education  Provide demonstration projects for innovative and effective management practices

ELIGIBILITY The following entities are eligible for CRWD Special Grants:  Government agencies serving the CRWD  Public and private schools located within the CRWD  Nonprofit, faith-based or religious organizations serving the CRWD  Private property owners who have highly visible locations that function like public spaces  Projects required to meet regulations are not eligible. Project elements that go above-and-beyond requirements are eligible.

Projects must be located within Capitol Region Watershed District, and only water quality improvement elements of projects are eligible for funding. Special Grants are primarily intended for public projects.

Private projects will be evaluated based on public benefit. Projects under construction or already completed prior to application are not eligible for funding. CRWD may offer to fund all, or a portion of the requested amount.

APPLICATION SUBMITTAL Please go to CRWD’s website to download the electronic application (www.capitolregionwd.org).

Applications must be submitted to CRWD no later than 4pm on Monday, October 31, 2016. Please submit applications via email to Nate Zwonitzer, Water Resource Project Manager (nate@capitolregionwd.org), or mail (Attn: Nate). The application form consists of a narrative, project budget, estimated timeline, and pollution reduction estimates. A description of the information to be included in the application is below.

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

Section 1: Applicant information  Agency/Organization – the entity responsible for receiving grant funds and implementing the project  Contact Person – Primary contact person for grant and project information (typically person authorized to execute grant agreement)  Project Name – Provide a name for the project that describes what will be done (10 words or less)  Project Location & Map – Provide an address, intersection or general description of the project area. Include a map with the application.

Section 2: Project Narrative The project narrative must fit in the two pages provided, and must include the following:  Grant project description and justification  Estimated water quality benefit (volume, phosphorus, and sediment reductions if applicable)  Estimated cost and funding sources (include CRWD grant request, owner/applicant contribution, other funding and sources)  Description of education and/or demonstration value  Description of level of innovation associated with the project  Description of monitoring and/or evaluation of effectiveness  Description of approvals and permits anticipated and ones that have been secured  Description of partnerships and landowner endorsements needed for the project  Include any developed project plans with the application (attach separately) Section 3: Project Budget and Timeline The table in Section 3 should be filled out for all phases of the project for which grant funds are requested. If a phase has been completed prior to application submittal, please note approximate date of completion and enter $0 under grant request (previously completed elements not eligible for grant funds).  Phase 1: Feasibility/Concepts – This phase should result in conceptual project designs that identify project location on the property, preliminary cost estimates, and pollutant reduction estimates. This phase includes soil investigations and identifies other potential project conflicts (utilities etc.). If the grant project is a study, a final report should be developed as part of this phase.  Phase 2: Construction Documents –Engineering/design needed to finalize concepts and cost estimates into construction-ready plans.  Phase 3: Bidding and Construction Management – Eligible activities include preparation of bid documents, bidding coordination, and construction management.  Phase 4: Construction – Construction costs to implement proposed projects. List each project element and its estimated cost. Examples of project elements include: east parking lot rain garden, stormwater reuse system for irrigation, permeable playground surface etc. Educational signage is included in the table and is required for construction projects.  Estimated Completion – Provide the estimated completion month/year for each phase  Grant Request – Provide the amount of CRWD grant funds requested for each phase and project element.  Applicant/Owner Match Funds – Provide the amount of match funds provided for each phase.

No match is required, but preference may be given to projects with dedicated funds. Match funds must be cash. Do not include in-kind labor in your match calculation.  Total Cost – This column auto-calculates and should be the total of the grant request and matching funds. It should reflect the estimated cost for each project phase/element.

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

REVIEW & APPROVAL Priorities All projects that meet eligibility requirements will be considered for funding. In addition, CRWD may prioritize projects that include the following:  Projects that utilize rainwater/stormwater harvesting  Projects that integrate stormwater management for transit oriented development along the Green Line light rail corridor.

Special Grant Project applications will be evaluated based on the following 4 major criteria: 1. Water Resource Management effectiveness and efficiency (40%) a. Project treatment area b. Impervious surface treatment area c. Volume reduction achieved d. Pollutant removal achieved e. Cost per volume/pollutant removed f. Located in a subwatershed with little or no existing treatment g. Natural resources protected and improved 2. Education and Demonstration Potential (25%) a. Public Location, Highly Visible b. Features/Elements of the Project specifically for demonstration/education c. Signage d. Community/Public Involvement e. Incorporate history or historic elements 3. Innovative Solution (25%) a. One of the first projects of its kind within CRWD b. Follow-up monitoring of effectiveness c. Likelihood of future acceptance and widespread implementation 4. Project Development and Partnerships (10%) a. Landowner endorsements, partnerships, and funding sources in place b. Required approvals and permits secured or will be secured c. Project likely to be implemented The District will evaluate the applications received based on the criteria listed above. Notification of preliminary approval for funding is intended to occur in December 2016. Final plans and specifications must be submitted to the District before construction begins. Funding will be on a reimbursement basis for actual costs as documented by paid invoices.

The applicant will have two years to complete and request reimbursement for the project once final approval has been granted. Extensions beyond the two years provided will require CRWD Board approval.

FUNDING Funding for 2016 Special Grants has not been set and the applications received during this solicitation will help determine the level of funding included in the District’s 2016 budget. In past years, the District Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

has approved a budget for Special grants of $300,000 - $500,000. Historically grant awards have ranged from $20,000--$200,000.

Questions If you have any questions about the CRWD Special Grant Program, requirements, or application process, please feel free to contact Nate Zwonitzer at 651-644-8888 or nate@capitolregionwd.org (email preferred).

\\CRWDC01\Company\06 Projects\Special Grants\2017\Application Materials\2017 Special Grant process.docx

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

GRANT APPLICATION FORM

Capitol Region Watershed District 1410 Energy Park Drive, Suite 4 Saint Paul, MN 55108 Capitolregionwd.org (651) 644-8888

Please submit form and required materials to: NATE ZWONITZER nate@capitolregionwd.org

PROJECT INFORMATION PROJECT NAME

PROJECT LOCATION: Please enter the complete address, intersection or description of the area where the project will be located.

SECTION 1

LOCATION

MAP ATTACHED

APPLICANT INFORMATION ORGANIZATION OR AGENCY

CONTACT PERSON

ZIP

CITY

ADDRESS

PHONE

EMAIL ADDRESS

PROJECT NARRATIVE

SECTION 2

1. WATER RESOURCE MANAGEMENT EFFECTIVENESS AND EFFICIENCY: PLEASE DESCRIBE THE PROJECT AND WHY IT IS IMPORTANT. INCLUDE WHAT IS BEING TREATED, POLLUTANT REDUCTION ESTIMATES, AND NATURAL RESOURCES PROTECTED OR IMPROVED.

SECTION 2

2. EDUCATION AND DEMONSTRATION POTENTIAL: PLEASE EXPLAIN HOW YOUR PROJECT WILL IMPROVE THE PUBLIC’S KNOWLEDGE OF NATURAL RESOURCE ISSUES AND SOLUTIONS. INCLUDE INFORMATION ON SPECIFIC EDUCATIONAL FEATURES, PROJECT VISIBILITY, OR PLANNED PUBLIC INVOLVEMENT.

SECTION 2

3. INNOVATIVE SOLUTIONS: PLEASE DESCRIBE HOW THIS PROJECT PROVIDES A UNIQUE APPROACH TO SOLVING A PROBLEM. INCLUDE PLANS TO MONITOR OR EVALUATE THE SUCCESS OF THE PROJECT.

4. PROJECT DEVELOPMENT AND PARTNERSHIPS: PLEASE DESCRIBE ANY WORK COMPLETED TO-DATE. INCLUDE ANY LANDOWNER ENDORSEMENTS, PARTNERSHIPS, AND FUNDING SOURCES ESTABLISHED. LIST ANY APPROVALS OR PERMITS THAT WILL BE REQUIRED OR ALREADY SECURED. HOW LIKELY IS THE PROJECT TO BE IMPLEMENTED?

PROJECT BUDGET AND TIMELINE COMPLETE THE FOLLOWING TABLE FOR ALL PROJECT PHASES FOR WHICH YOU WILL BE REQUESTING GRANT FUNDS. MATCH FUNDS ARE NOT REQUIRED, BUT PREFERENCE MAY BE GIVEN TO PROJECTS WITH DEDICATED FUNDS. MATCH FUNDS MUST BE CASH (IN-KIND LABOR NOT CONSIDERED). SEE THE 2015 SPECIAL PROJECT AND GRANT PROGRAM DESPRIPTION FOR DETAILED INSTRUCTIONS ON WHAT TO INCLUDE IN THE PROJECT BUDGET AND TIMELINE TABLE.

TASK

ESTIMATED COMPLETION

GRANT REQUEST

APPLICANT MATCH FUNDS

Phase 1:

TOTAL COST

$ 0.00

Feasibility/Concepts

$ 0.00 Phase 2:

$ 0.00

Construction Documents

SECTION 3

$ 0.00 Phase 3:

$ 0.00

Bidding & Construction Management

$ 0.00 Phase 4:

$ 0.00

Construction (List Project Elements Below)

$ 0.00

Educational Features/Signage

$ 0.00

$ 0.00

$ 0.00 $ 0.00 $ 0.00 TOTAL

$ 0.00

$ 0.00

$ 0.00

September 7, 2016 Board Meeting V. Action Item, E) Award Bid for Willow Reserve Restoration Project (Zwonitzer)

DATE: TO: FROM: RE:

August 29, 2016 CRWD Board of Managers Nate Zwonitzer, Water Resource Project Manager Award Bid for the Willow Reserve Restoration Project

Background In July, CRWD’s Board of Managers authorized solicitation of bids for the Willow Reserve Restoration Project. Willow Reserve is a 23-acre bird and wildlife reserve located off Maryland Avenue between Arundel and Virginia Streets in Saint Paul that is owned and managed by the City of Saint Paul. CRWD, Saint Paul and District 6 Planning Council will work together to achieve the following goals for the site: restore ecological integrity, maximize urban wildlife, increase native vegetative species diversity, improve pond water quality and maintain the reserve’s natural and passive setting. The project was estimated to cost $190,000 with restoration work occurring from 2016-2019.

Issues Bidding for the project extended over a three-week period from August 4 to August 25, 2016. CRWD received bids from two contractors: 1) Minnesota Native Landscapes - $262,410; 2) Wetland Habitat Restorations (WHR) - $151,907. Based on the lowest bid from WHR and CRWD’s experience working with them on vegetation management at Highland Ravine, CRWD staff recommends the Board award the project to WHR. Action Requested Authorize the Board President and Administrator to execute a Notice of Award and an Agreement with Wetland Habitat Restorations for the Willow Reserve Ecological Restoration Project subject to the review and approval of the Ramsey County Attorney; and authorize the Administrator to execute change orders in an amount not to exceed $10,000.

\\CRWDC01\Company\06 Projects\Willow Reserve\Restoration Implementation\Bidding and Specs\Bd memo Willow Reserve Award 09-7-16.docx

Our Mission is to protect, manage and improve the water resources of Capitol Region Watershed District.

September 7, 2016 Board Meeting

DATE: TO: FROM: RE:

September 1, 2016 CRWD Board of Managers Maddie Vargo, Water Resource Technician 2016 Quality Assurance Program Plan (QAPP)

V. Action Items F. AR: Approve Monitoring Quality Assurance Program Plan (Vargo)

Background CRWD has been collecting and analyzing water quality data through the District Monitoring Program since 2005. The primary objective of the CRWD monitoring program is to identify water quality problem areas, to quantify pollutant runoff to the Mississippi River, and to promote understanding of District water resources.

CRWD has conducted its monitoring operations at 127 unique sites over the program’s 11-year history.

Since implementation of the monitoring program in 2005, incremental improvements and enhancements have been made. In 2013, the District completed a comprehensive review of the program to analyze refocus the goals, approach, methods, and deliverables of the Program. The Board of Managers approved the Monitoring Program Review and 2014-2016 Work Plan in April 2014, in which CRWD staff put forth Recommendation 9 to: “Improve methods and efficiency of sample preparation and lab analysis including…. finalize Quality Assurance and Program Plan (QAPP).”

Issues The QAPP guides a monitoring program by: a) defining data quality assurance goals and procedures b) summarizing the program design, sampling methods, analytical procedures, and data review protocols.

The contents of a QAPP ensure that quality assurance objectives and regulatory needs are being met.

Monitoring data collected using an approved QAPP have strong credibility with outside parties and allow the district to confidently utilize the data to make regulatory decisions.

The CRWD QAPP will be updated annually to reflect any changes made in the monitoring program. CRWD Staff will review the 2016 QAPP with the Board of Managers.

If you would like a printed copy of the QAPP please contact the CRWD office staff.

Requested Action Accept the 2016 Quality Assurance Program Plan.

W:\07 Programs\Monitoring & Data Acquisition\Monitoring Protocols\Quality Assurance Program Plan (QAPP)\2016 QAPP\Board Mtg_09-07-16\Board Memo_2016 QAPP 09-07-16

Monitoring Quality Assurance Program Plan Capitol Region Watershed District – St. Paul, Minnesota

September 2016

By my signature below, I attest that I am familiar with the requirements of this document and agree to fulfill the responsibilities specified herein.

_____________________________________________ Bob Fossum, CRWD Monitoring Program Manager,

____________________ Date

_____________________________________________ Britta Suppes, CRWD Monitoring Coordinator

____________________ Date

1

Table of Contents 1.0 Introduction ............................................................................................................................................. 5 1.1 Program Background .................................................................................................................. 5 1.2 Program Description ................................................................................................................... 5 1.3 Quality Objectives and Criteria ....................................................................................................... 8 1.4 Monitoring Variables and Frequency.............................................................................................. 8 2.0 Program Organization and Responsibilities .......................................................................................... 10 2.1 Capitol Region Watershed District Responsibilities ................................................................. 10 2.2 2.3

Laboratory Service Responsibilities ......................................................................................... 11 Stakeholders, Partners, and Agencies Responsibilities ............................................................. 11

2.4 Relationship of QAPP to Other Guidance Documents ................................................................. 11 3.0 Field Measurement Equipment ............................................................................................................. 12 3.1 ISCO 6712 Automated Sampler ............................................................................................... 12 3.2 ISCO 2100 Flow Modules ........................................................................................................ 13 3.3 ISCO Model 750 ........................................................................................................................... 13 3.4 Field Equipment Maintenance and Calibration ............................................................................. 14 4.0 Field Sampling Methods ....................................................................................................................... 14 4.1 Field Activity Preparation and Field Decisions ........................................................................ 14 4.2

Sampling Procedures ................................................................................................................ 15 4.2.1 Flow-Weighted Composite Sampling Procedure ......................................................... 15 4.2.2

4.3

4.4

Grab Sampling Procedures .......................................................................................... 16

Sample Bottle Preparation and Equipment Cleaning ................................................................ 18 4.3.1 4.3.2

ISCO Sampler Bottles and Equipment Cleaning ......................................................... 18 MCES Laboratory Submission Containers and Preservatives ..................................... 19

4.3.3

Private Laboratory Submission Containers and Preservatives..................................... 19

QA/QC Sampling Methods ....................................................................................................... 19 4.4.1

Equipment Blank Sampling Method ............................................................................ 20

4.4.3

Composite Duplicate Sampling Method ...................................................................... 20

4.4.4 Grab Replicate Sampling Method.................................................................................... 21 5.0 Quality Assurance Objectives ............................................................................................................... 22 5.1 Precision.................................................................................................................................... 23 5.2 5.3

Accuracy ................................................................................................................................... 24 Completeness ............................................................................................................................ 24

5.4

Sensitivity ................................................................................................................................. 25

5.5

Comparability ........................................................................................................................... 26

5.6

Representativeness .................................................................................................................... 27

6.0 Field and Sample Custody Documentation ........................................................................................... 27 6.1 Field Forms ............................................................................................................................... 27 6.2

Analytical Data Review ............................................................................................................ 29

2

6.3

Sample Identification ................................................................................................................ 29

6.4 6.5

Chain of Custody ...................................................................................................................... 29 Sample Handling and Transport ............................................................................................... 30

7.0 Quality Assurance Procedures .............................................................................................................. 30 7.1 Laboratory Quality Assurance Procedures ............................................................................... 30 7.2 CRWD Quality Assurance Procedures ........................................................................................ 31 7.2.1 7.3

Data Management ........................................................................................................ 31

7.2.2 Data Review and Validation ........................................................................................ 32 Corrective Action ...................................................................................................................... 36

8.0 Data Assessment and Reporting ........................................................................................................... 36 8.1 Internal Quality Assessments .................................................................................................... 36 8.2

Electronic Data Reporting......................................................................................................... 37

8.3

Annual Monitoring Report ........................................................................................................ 37

9.0 References ............................................................................................................................................. 38

3

List of Tables Table 1

Monitoring Station Location

Table 2

Monitoring Parameters, Minimum Frequencies, and Typical Frequencies

Table 3

CRWD Monitoring Personnel

Table 4

Duplicate and Replicate Naming Conventions for CRWD Full Water Quality Sites

Table 4

Types of Equipment Blanks Performed by CRWD

Table 6

MCES Quality Control Methods

Table 7

Laboratory Sample Parameters, Analytical Method, and Holding Times

Table 8

WISKI Quality Codes and Standard Remarks

Table 9

Steps for Various Data Editing Scenarios List of Figures

Figure 1

Monitoring Station Location Map

Figure 2

Monitoring Flow Chart List of Attachments

Appendix A

CRWD Organizational Chart

Appendix B

Field Standard Operating Procedures

Appendix C

Sample Chain of Custody

Appendix D

Laboratory Quality Assurance Manual and Standard Operating Procedures Distribution List

Name

Title/Organization

Mark Doneux

Administrator, CRWD

Bob Fossum

MRM Division Manager, CRWD

Britta Suppes

Monitoring Coordinator, CRWD

Joe Sellner

Water Resource Technician, CRWD

Sarah Wein

Water Resource Technician, CRWD

Maddie Vargo Anna Eleria

Water Resource Technician, CRWD Planning, Projects, and Grants Program Manager CRWD

Nate Zwonitzer

Water Resources Project Manager, CRWD

Gustavo Castro

Water Resource Specialist, CRWD

Elizabeth Hosch

BMP Inspector, CRWD

4

Address 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108 1410 Energy Park Drive, St. 4, St. Paul, MN 55108

E-mail mark@capitolregionwd.org bob@capitolregionwd.org britta@capitolregionwd.org joe@capitolregionwd.org sarah@capitolregionwd.org maddie@capitolregionwd.org anna@capitolregionwd.org nate@capitolregionwd.org Gustavo@capitolregionwd.org ehosch@capitolregionwd.org

1.0 Introduction This Monitoring Quality Assurance Program Plan (QAPP) guides water quality and quantity monitoring for the Capitol Region Watershed District (CRWD). It outlines sample collection and analysis procedures.

1.1

Program Background

CRWD is a special purpose unit of government in Ramsey County, Minnesota that manages and protects water resources within its watershed boundaries. CRWD is a 41 square mile watershed nested in the Upper Mississippi River basin that contains portions of five cities, including Falcon Heights, Lauderdale, Maplewood, Roseville, and Saint Paul. CRWD is highly urbanized with a population of 245,000 and 42% impervious surface coverage. All runoff from CRWD eventually discharges to the Mississippi River from 42 outfall locations.

The water resources within the District are monitored by CRWD in addition to other partnering entities. CRWD monitors streams, stormwater, and best management practices (BMPs) within the District. Lakes water quality monitoring is completed by Ramsey County Public Works (RCPW).

The Mississippi River is monitored by Metropolitan Council Environmental Services (MCES), the United States Geological Survey (USGS), the Army Corps of Engineers, and the Minnesota Pollution Control Agency (MPCA). The City of St. Paul also monitors stormwater water quality and BMP performance at several locations throughout the District.

The primary objectives of the CRWD monitoring program are to identify water quality problem, quantify the subwatershed runoff pollutant loadings to the Mississippi River or other receiving water bodies, evaluate the effectiveness of BMPs, provide data for the calibration of hydrologic, hydraulic, and water quality models, and promote understanding of District water resources and water quality.

CRWD also conducts stormwater monitoring to assist the City of St. Paul in meeting the monitoring requirements for the Phase I Municipal Separate Storm Sewer System (MS4) Permit (MPCA, 2016).

1.2

Program Description

CRWD monitors sites for level, velocity, and flow during the monitoring season, which is generally March to November. Monitoring sites that have continuous baseflow through the winter months are monitored year-round. Samples are collected with automated samplers at most sites during the monitoring season and with grab samples during the winter months. Samples collected by CRWD

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are sent to an analytical laboratory for analysis. Data are summarized and analyzed in an annual monitoring report.

Table 1 and Figure 1 show monitoring station locations within the district. The 2016 monitoring program includes 18 full water quality monitoring sites. Full water quality sites are broadly categorized as either a baseflow or non-baseflow. A baseflow site has constant flow throughout the year. Flows typically vary, depending on groundwater levels, surface water levels, and other factors.

These sites receive inputs from stormwater and snowmelt as well. A non-baseflow site only has flow when there are external inputs in the system, such as a storm, snowmelt, or an illicit discharge event.

Grab samples are taken at 7 sites listed in Table 1, generally within 24 hours of a storm event. Grab samples may also be taken at baseflow and non-baseflow sites when composite samples are not able to be taken (Section 4.2.2). E.coli grab samples are taken at all full water quality station during storm events when staff were able to visit stations, and during monthly base composite sampling events.

Flow-only stations are installed at 9 sites to record 15-minute level, velocity, and temperature data.

Level loggers are installed at 14 sites to record 15-minute level data. No water quality samples are taken at these locations.

Table 1: Monitoring Station Locations (2016).

Full Water Quality

Baseflow

Flow Logger

Level Logger

Grab Sample

Non-Baseflow

St. Anthony Park

AHUG Inlet

Como Outlet

AHUG Level

William St. Pond

Hidden Falls Outlet

Como 7

McCarrons Outlet

Arlington-Jackson

Willow Reserve

East Kittsondale

Como 3

TBNS - Magnolia

Arlington-Jackson S. Bay

Upper Villa-West Exfiltration

Phalen Creek

Upper Villa Inlet

TBNS - Jenks

Como Lake Level

Upper Villa-Center Exfiltration

Trout Brook Outlet

Franklin

Upper Villa Outlet

Crosby Lake

Upper Villa-East Exfiltration

Trout Brook-East Branch

Dickerman Park

VP-Out Overflow Channel

Curtiss Pond Groundwater

GRS Jellyfish-In

Trout Brook-West Branch

MLK

Swede Hollow

Golf Course Pond

GRS Jellyfish-Out

Villa Park Outlet

TBNS-Rose

TBO-SP Tunnel

Lake McCarrons

Villa Park Inlet

TBNS-Stream

TBI West-TB Circle

Sims-Agate Victoria Park Pond VP-Inlet Pond Westminster-Miss William Street Pond Willow Reserve

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Figure 1: Monitoring Station Location Map.

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1.3 Quality Objectives and Criteria The primary goal of this QAPP is to define the data quality assurance goals and quality assurance procedures that are applicable to the monitoring program. The CRWD monitoring program strives to produce consistent and reliable data; however, non-routine situations may arise for rapid response monitoring or monitoring a new location. This plan includes best practices that can be applied to both routine and non-routine monitoring circumstances.

Monitoring data results may indicate environmental and/or human health concerns that require corrective actions and/or long-term management decisions. Therefore, sampling and analysis must meet specified quality assurance and quality control objectives to accurately characterize the conditions within the district. This QAPP reflects the need for data of acceptable quality, while recognizing financial and technical constraints. The detailed data quality assurance objectives are discussed later in this plan.

1.4 Monitoring Variables and Frequency The types of samples collected by the CRWD monitoring program are determined by the time of year, type of flow, and method of collection (Figure 2). Automated samplers for collecting water quality samples are installed during the field season, roughly March to November. Event samples are collected during and/or after storm events, either as flow-weighted composite samples or as grab samples. Baseflow samples are taken as composites on a monthly basis during the field season as conditions allow. Baseflow samples are also taken during winter as grab samples. Snowmelt grab samples are also taken if there are two consecutive days above freezing, and flow is entering the storm sewer. Snowmelt grab samples are taken at non-baseflow sites when conditions allow.

Composite, after event Event flow Grab, during event

Field Season (March - November) Baseflow (baseflow sites only)

Composite, monthly

Baseflow (baseflow sites only)

Grab, monthly

Snowmelt

Grab, as available

Winter

Figure 2: Monitoring Flow Chart.

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The suite of parameters tested under the CRWD monitoring program are listed in Error! Reference source not found.. Water quality samples are analyzed for specific parameters generally at a minimum frequency defined in the City of St. Paul’s MS4 Permit. Parameter analysis is also dependent on the time since the sample was collected (holding time), available sample volume, and water quality concerns for a site.

Table 2: Monitoring Parameters, Minimum Frequencies, and Typical Frequencies.

Parameter

Sample Type

Frequency

Typical Frequency

(required by MS4 permit)

Ammonia Nitrogen

Composite

Quarterly

35 per year

BOD - Carbonaceous 5-day

Composite or Grab

Quarterly

Quarterly

Cadmium

Composite or Grab

Not Required

35 per year

Chloride

Composite or Grab

15 per year

35 per year

Chromium

Composite or Grab

Not required

35 per year

Copper

Composite or Grab

Monthly

35 per year

E. coli

Grab

Quarterly

22 per year

Flow (from level and velocity)

Measurement

Every 15 minutes with sampler installed

Fluoride

Composite or Grab

Every 15 minutes with sampler installed Not Required

Hardness

Composite or Grab

Monthly

4 per year (For illicit discharge monitoring) 35 per year

Lead Nickel

Composite or Grab Composite or Grab

Monthly Not Required

35 per year 35 per year

Nitrite Plus Nitrate, Total (as N) Ortho Phosphate

Composite Composite

15 per year Quarterly

35 per year Quarterly

pH

Composite or Grab

Quarterly

Quarterly

Potassium

Composite or Grab

Not Required

Sulfate

Composite or Grab

2 per Year

4 per year (For illicit discharge monitoring) Quarterly

Surfactants

Composite or Grab

Not Required

Total Dissolved Solids

Composite

Quarterly

4 per year (For illicit discharge monitoring) 35 per year

Total Kjeldahl Nitrogen

Composite

15 per year

35 per year

Total Phosphorus

Composite

15 per year

35 per year

Total Suspended Solids

Composite

15 per year

35 per year

Volatile Suspended Solids

Composite

15 per year

35 per year

Zinc

Composite or Grab

Monthly

35 per year

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2.0 Program Organization and Responsibilities Personnel associated with the program and their contact information are presented in Table 3.

Monitoring is overseen by the program manager and monitoring coordinator. Water resource technicians are responsible for the majority of the field work, with occasional help from other CRWD staff. Technicians are also responsible for reviewing data and assessing the QA/QC practices outlined in Section 4.4. The complete CRWD organizational chart can be found in Appendix A.

Table 3: CRWD Monitoring Personnel.

Position

Name*

Organization

Phone Number

Program Manager

Bob Fossum

CRWD

651-644-8888

Monitoring Coordinator

Britta Suppes

CRWD

651-644-8888

Quality Assurance Manager

Anna Eleria

CRWD

651-644-8888

Water Resource Technician

Joe Sellner

CRWD

651-644-8888

Water Resource Technician

Sarah Wein

CRWD

651-644-8888

Water Resource Technician

Maddie Vargo

CRWD

651-644-8888

* Staff are subject to change

2.1

Capitol Region Watershed District Responsibilities

CRWD staff are responsible for coordinating and conducting field operations for the monitoring program and meeting the quality assurance objectives outlined by the QAPP. District staff install, operate, and maintain monitoring equipment at each of the monitoring stations, collect water quality samples, download hydrologic data, select new sites for monitoring, and remove sites no longer needed. District staff is also responsible for reviewing analytical laboratory and hydrologic (level, flow, and discharge) data for quality assurance. CRWD is responsible for preparing annual work plans and scoping documents, filling out sample chain of custody (COC) documents, preparing annual reports, and submitting data to the City of St. Paul, and other partners and stakeholders.

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2.2

Laboratory Service Responsibilities

Samples are currently submitted to Metropolitan Council Environmental Services (MCES) for analysis. The analytical laboratory is responsible for receiving samples from CRWD, filling out chain of custody documents, and analyzing samples. The analytical laboratory is also responsible for ensuring all QA/QC procedures are in place and followed for all laboratory functions. The analytical laboratory’s QA/QC procedures, including organizational structure, laboratory procedures and qualifications, can be referenced in the Laboratory Quality Assurance Management Plan in Appendix C. Other qualified analytical laboratories may be contracted to perform the routine analytical work or work beyond the scope of this QAPP. Prior to contracting with other laboratories for analytical services, CRWD staff will review their Quality Assurance Manual and ensure it is consistent with the CRWD QAPP. Laboratory reports, containing results of analytical analysis are accessed by CRWD from the MCES database once per month. Analytical analysis records are saved electronically to the CRWD server.

2.3

Stakeholders, Partners, and Agencies Responsibilities

CRWD’s Board of Managers, Citizen’s Advisory Committee, and the general public provide input on monitoring activities. Other units of government and private companies suggest possible areas of collaboration or express areas of environmental concern, which also helps direct the monitoring program. Citizen groups, neighborhood groups, and city councils have historically been sources of direction for monitoring priorities. The MPCA, research institutions (e.g. the University of Minnesota), and MCES review annual reports and data. The cities of St. Paul, Falcon Heights, Roseville, Lauderdale, and Maplewood review annual reports as well.

2.4 Relationship of QAPP to Other Guidance Documents This QAPP is one of a number of documents that guide the CRWD monitoring program. Contained in this QAPP are summaries of the program design, sampling methods, analytical procedures, and data review protocols. CRWD Standard Operating Procedures (SOPs) provide a more in-depth description of sampling procedures, field analysis laboratory analysis, and data review (Appendix B).

This QAPP and aforementioned SOPs are available to staff for electronic review on the CRWD server and for tangible review in the office.

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3.0 Field Measurement Equipment This section outlines the field measurement equipment used in CRWD’s stormwater monitoring program. Site equipment is either housed in a monitoring box enclosure or hung in a manhole by a suspension bracket. Each water quality monitoring site consists of an automated sampler with intake tubing and sieve, a flow logger with a sensor, and a power source (i.e. deep-cycle battery, 6V battery). CRWD uses two different sizes of ISCO 6712 samplers: compact and full-sized. For measuring and recording level and flow data, ISCO 2100 series flow modules or ISCO 750 area velocity flow modules are used. At each site, level and velocity are recorded by the flow logger in 5 or 15 minute intervals by a sensor that is mounted on a plate in the center of the channel or pipe.

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Other standard equipment for each monitoring site includes a 12-volt marine battery and connection cables for 6712 units. Periodic inspections of field measurement equipment and their components will be performed in order to assure their use as specified in their respective manuals and this QAPP.

3.1

ISCO 6712 Automated Sampler

ISCO 6712 automated samplers are used to collect flow-weighted composite samples during the monitoring season. Sample volume calibrations are done when the sampler is installed, and as needed (Section 3.4). The procedure for flow-weighted composite sampling is described in Section 4.2.1.

Two different sizes of 6712 automated samplers are used: •

ISCO 6712 Full-Size Portable Sampler: 24 1-liter ISCO sampler bottles. Collects 4 200-ml samples per bottle, for a total of 96 samples.

•

ISCO 6712c Compact Portable Sampler: 24 500-ml ISCO sampler bottles. Collects 2 200ml samples per bottle, for a total of 48 samples.

Internal desiccant packets are replaced annually. The ISCO 6712 sampler instruction manual, available in the office and online, provides directions for operating, maintaining, and calibrating samplers (ISCO, 2015).

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3.2

ISCO 2100 Flow Modules

ISCO 2100 flow modules measure water level at 15-minute intervals. While samplers are enabled, measurements are taken at 5-minute intervals. Most sites are equipped with a 2100 stack built from a 2150, 2105, and a 2191 module ISCO 2150 modules log flow data measured by an area-velocity sensor that is mounted in the center of the channel or pipe ISCO 2105 modules interface between the sampler and the 2150 area velocity module, triggering the sampler to collect samples based on a preprogrammed water level threshold as measured by the sensor. ISCO 2191 modules house battery units to power the stack.

Velocity measurements are also recorded at 15-minute intervals when the samplers are not enabled and recorded at 5-minute intervals when enabled. Velocities are measured by the 2150 sensor by transmitting a continuous ultrasonic wave, and then measuring the frequency shift of returned echoes reflected by air bubbles or particles in the flow.

Desiccant indicators located inside the ISCO 2150, 2105, and 2191 modules are inspected regularly and exchanged as needed. The ISCO 2150 module instruction manual, available in the office and online, provides directions for operation, maintenance, and calibration (ISCO, 2012a & ISCO 2012b).

3.3 ISCO Model 750 Measurements of water level are recorded at 15-minute intervals, measured using submerged pressure transducers that are mounted in the flow stream. 750 modules have no temperature coefficient calibration.

Velocity measurements are recorded at 15-minute intervals. Velocities are measured using sensors transmit a continuous ultrasonic wave, and then measure the frequency shift of returned echoes reflected by air bubbles or particles in the flow.

Desiccant cartridges are inspected at each site visit, and exchanged as needed. The ISCO Model 750 instruction manual, available in the office and online, provides directions for operation, maintenance, and calibration (ISCO, 2013).

13

3.4 Field Equipment Maintenance and Calibration CRWD staff monitor the performance of equipment and instruments in the field during routine site visits. CRWD staff will work to resolve deficiencies as they are discovered, and replace components as needed. If necessary, equipment can be tested in the CRWD shop using a vertical level testing apparatus and/or a velocity testing flume. Equipment may be sent back to the manufacturer if deficiencies cannot be resolved by CRWD staff. The Monitoring Coordinator will provide the final decision on the usability of damaged equipment.

ISCO 6712 samplers require frequent attention to assure their intended use. Intake tubing is replaced annually to prevent sample contamination. Pump arm tubing is replaced after 1,000,000 turns of the sampler pump.

ISCO 6712 samplers and attached modules record accurate level measurements and pump precise sample volumes. These mechanisms must be calibrated at the time of installation and as needed throughout the field season to maintain their accuracy. To calibrate sample volumes, the sampler is manually triggered to pump a particular volume. The pumped volume is measured in a graduated cylinder. The process is repeated until the desired sample volume is attained. 2150 level sensors are calibrated once per month while installed. To calibrate the level, a reference stage is manually measured in the flow in front of the sensor with a ruler. This is compared against the measurement logged by the 2150 module. If the module measurement and the reference measurement differ by more than 0.1 foot, the instrument stage is re-calibrated to equal the reference stage measurement.

4.0 Field Sampling Methods This section defines procedures to be used for collecting and handling water quality samples.

Unforeseen circumstances may require deviations from these procedures. Such deviations will be approved by the program manager or the monitoring coordinator prior to sampling. If prior approval cannot be obtained, deviations from the established procedures will be recorded and needs for resampling will be evaluated.

4.1

Field Activity Preparation and Field Decisions

Upon arrival at each monitoring location, field conditions are noted in the electronic field form. Any unusual condition will be recorded on the electronic field form. Conditions that may interfere with 14

obtaining representative analytical results will be rectified before sampling proceeds and noted in the field form.

Minor changes to the field protocol can be made by the field technicians if prior approval cannot be obtained. Changes will be reviewed with the monitoring coordinator retroactively to decide whether or not locations need to be re-sampled. For significant changes to this protocol, approval will be obtained in advance from the program manager. The program manager will review any changes to the protocol that may adversely affect results before proceeding. Any deviations, minor or significant, are reviewed by the monitoring coordinator and by the program manager during the writing of the annual report.

4.2

Sampling Procedures

CRWD collects two different types of samples: flow-weighted composite samples and instantaneous grab samples. Composite samples are generally taken for baseflow, storm, and illicit discharge events during the monitoring season. Grab samples are taken when samplers are not installed or malfunctioning during the monitoring season. E. coli samples are always taken as grab samples.

4.2.1

Flow-Weighted Composite Sampling Procedure

Composite sampling is conducted to collect a representative sample of a base, storm, or illicit discharge event. The following equipment is used to collect composite samples: •

24 Clean 1000-ml or 500-ml plastic ISCO sampler bottles and caps

•

ISCO 6712 Full Size Portable Sampler or 6712c Compact Portable Sampler

•

14-liter churn, clean

•

Clean, labeled, 4-liter laboratory submission container, or various laboratory submission containers with preservatives for private laboratory

•

Cooler

The samplers are programmed (paced) to collect a discrete sub-sample for a given volume of water that passes through a channel. Discrete 200-ml sub-samples are collected into 500-ml ISCO sampler bottles in compact samplers (48 sub-samples maximum per sampler), and into 1-liter ISCO sampler bottles in standard samplers (96 sub-samples maximum per sampler).

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To collect storm composites, samplers are programmed with triggers and pacings to collect discrete sub-samples of the event. Trigger and pacing values vary throughout the season, depending on flow conditions, antecedent moisture conditions, expected rainfall amounts, and other factors. If an illicit discharge is suspected, a sampler can be programed with triggers and pacing in an attempt to collect a representative sample.

Technicians retrieve full or partially full carousels from sites with automatic samplers following a sampling event. Staff place ISCO sampler bottle lids on bottles and transport them to the CRWD office to be composited.

A churn is used to composite samples from individual bottles into one homogenous sample. Each ISCO sampler bottle in a carousel is vigorously shaken to agitate any sediment that has settled out.

The contents are dumped into a 14 L churn and the composited sample is mixed for 30 seconds.

While being continuously mixed, the sample is released into a laboratory submission container.

Composited samples are then placed in a refrigerator at about 4 degrees Celsius. Within 24 hours of compositing, samples are placed in coolers, and transported to the analytical laboratory for analysis.

If a carousel of samples exceeds the 14 L churn volume, sample bottles are divided into two sample sets. The above process is followed for each sample set; but, only 2,000 mL of each composited sample sets the composited samples are distributed into the 4,000 mL submission containers.

Base composite samples are collected monthly while ISCO samplers are installed. Samplers are paced in order to collect a full carousel (24 bottles) in approximately 24 hours at base flow levels.

Samples are collected after the 24-hour cycle and taken back to the CRWD office to be composited using the same procedures for compositing storm samples.

4.2.2

Grab Sampling Procedures

Grab sampling is done in conjunction with composite sampling or as a standalone procedure. The following equipment is used to collect grab samples: •

Sampling bucket and rope, clean

•

Clean, labeled, 4-liter laboratory submission container, or various laboratory submission containers with preservatives for private laboratory

•

ISCO 6712 Full Size Portable Sampler or 6712c Compact Portable Sampler pump, or other external pump (Pump Collection method only) 16

•

Cooler and ice

If no composite sample is taken due to equipment failure, a grab sample can be taken instead. At sites without an ISCO 6712 sampler installed, grab samples are collected. Outside of the monitoring season, base grab samples are taken once per month. Snowmelt grab sampling and illicit discharge grab sampling are also conducted when possible. E. coli grab sampling is conducted once per month.

Grab samplers are taken from the middle of the flow to collect well-mixed samples. The sample is then poured directly into a clean laboratory submission container. The container is capped, stored in a cooler, and sent directly to the lab for analysis. Alternatively, samples can be transported back to CRWD, placed in the refrigerator, and brought to the lab within 24 hours for analysis. Three variations of full water quality grab sampling and one version of E. coli grab sampling methods are discussed below.

4.2.2.1

Bucket and Rope Sampling

Bucket and rope sampling is the most common way to collect full water quality grab samples, since many sites are below ground and most easily accessed from the surface. A sampling bucket with rope is lowered from the surface and triple-rinsed with sample water. The samples are then collected and poured directly into clean laboratory submission containers.

4.2.2.2

Direct Grab Sampling

Samples are occasionally collected directly from the water source if the water body is at the surface or staff has used confined space entry to access an underground channel. Similar to bucket and rope sampling, the sampling bucket should be triple-rinsed and poured into clean laboratory submission containers. If taken from a stream, samples should be taken facing upstream, from the center, while ensuring that no stream bottom debris is disturbed in the process. If taken from a pond, samples should be taken from a consistent location, slightly below the surface, with floating vegetation moved away, and while ensuring no pond bottom debris is disturbed in the process. If taken from an outlet structure, samples should be taken as they flow over the outlet structure. Occasionally, the laboratory submission container may be filled directly from the outlet structure.

4.2.2.3

Pump Collection

If the above grab sampling methods are not possible, a grab sample can be collected using the ISCO 6712 pump or other external pump. To use this method, the sampler is set to pump in reverse and the sampling line is purged for 30 seconds. Then, the sampler is set to pump forward for 15 seconds

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before filling a laboratory submission container. This method is often employed at East Kittsondale due to the slight offset of the mahhole from the storm sewer.

4.2.2.4

E. coli Grab Sampling

E. coli grab samples are taken in conjunction with base composites, base grabs, storm composites, storm grabs, snowmelt grabs, and illicit discharge samples. The following equipment is used to collect E. coli grab samples: •

E. coli Sampler and Rope

•

NASCO Whirl-Pak® Sample Bags, or other E. coli laboratory submission containers

•

Small cooler with ice pack

A sterile, 18-ounce NASCO Whirl-Pack® is opened on-site and placed in a sampler. Care is taken to ensure the Whirl-Pack® is not contaminated by field staff fingers, sewer side walls, or other contaminants. In some circumstances, the Whirl-Pack® can be filled without the sampler. If this is the case, the Whirl-Pack® is filled with sample water while being held at the tabs, with care taken to ensure it is not contaminated by field staff fingers or other contaminants. The sample must be taken directly from the water source. The sample is put in a small cooler with ice packs immediately and transported to the laboratory within 6 hours.

4.3

Sample Bottle Preparation and Equipment Cleaning

4.3.1

ISCO Sampler Bottles and Equipment Cleaning

Flow-weighted composite samples are collected into either 500-ml or 1000-ml carousel ISCO sampler bottles. To decontaminate used bottles, they are washed in a Liqui-Nox and hot water solution, scrubbed with a wire brush, and loaded into the dishwasher. Unused bottles that shared a carousel with bottles containing samples are also loaded into a dishwasher, without pre-washing.

Caps are also dunked in a Liqui-Nox and hot water solution. They are then placed in the dishwasher in silverware containers, not to be more than ¾ full. The dishwasher is run without soap on the “Pots and Pans” setting to sterilize the bottles. Clean bottles are loaded into carousels and stored upsidedown on a shelf in the CRWD equipment shop. Clean caps are separated by size and stored in clean containers.

Once all bottles have been removed from the sampler carousel bases, the bases are rinsed with tap water, scrubbed (if needed), and placed out to air dry.

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Churn components are triple-rinsed in tap water after each sample is composited. After all samples have been composited for the day, churns are washed with Liqui-Nox and hot water, rinsed, and loaded into the dishwasher. The dishwasher is run without soap on the “Pots and Pans” setting to sterilize the churns.

4.3.2

MCES Laboratory Submission Containers and Preservatives

Composited samples and grab samples are deposited in 4-liter containers to be submitted to MCES. MCES neither requires CRWD to preserve samples nor to transport samples on ice (with the exception of E. coli samples). Prior to delivery, samples may be stored in a refrigerator at approximately 4 degrees Celsius for up to 24 hours.

At the laboratory, MCES preserves some samples in order to process certain parameters. MCES’ Quality Assurance Manual (QAM) includes specific procedures for the following: laboratory submission container cleaning, testing, labeling and storage, and preparation (Appendix D).

4.3.3

Private Laboratory Submission Containers and Preservatives

CRWD primarily uses the MCES laboratory for sample analysis; however, private laboratories may be used periodically for various sample analyses. Private labs may be used for the following reasons: •

CRWD intent to investigate alternative labs

•

Need for specialized sampling parameters for which MCES is not equipped to perform analysis (e.g. gross solids)

•

MCES unable to process samples due to logistical constraints

The private laboratory QAM shall include specific procedures for the following: sample container cleaning, testing, labeling and storage, preparation, and addition of preservatives. All chemical preservatives added to containers in the laboratory will meet the criteria of the laboratory’s QA/QC program as reflected in the QAM.

4.4

QA/QC Sampling Methods

This section defines specific quality control samples that will be collected in the field. Equipment blanks, field blanks, and field duplicate samples are submitted to the analytical laboratories to provide the means to assess the quality of the data resulting from the field sampling procedures.

How these samples are used to meet the quality assurance objectives is discussed further in Section 5.0.

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4.4.1

Equipment Blank Sampling Method

Equipment blanks verify the effectiveness of the equipment decontamination procedures. Equipment blanks are conducted after various pieces of equipment employed by the CRWD monitoring program are cleaned. To collect an equipment blank, analyte-free, deionized water is poured into or through the container in question. Information regarding the types of blanks, associated frequency, and when the blank will be conducted can be found in Table 4. Blank samples must account for 10% of all samples submitted, which equates to the frequencies listed in Table 4 for an average sampling year.

Equipment blank samples will be identified according to a table of aliases listed in the “Equipment Blank SOP,” which can be found in Appendix B.

Table 4: Types of Equipment Blanks Performed by CRWD. Blank Type

Frequency

When to Complete

ISCO Sampler Blank

3 sites,3 times/year

Grab Sampler Blank

5 times/year

During level calibrations In the field after a suite of grab samples, after a triple rinse of DI water

Churn Blank Sampler Bottle Blank

10 times/year

In the shop after triple rinse of tap water

10 times/year

After cleaning process and dishwashing cycle

MCES Bottle Blank

10 times/year

Anytime

Equipment blanks are analyzed for the target parameters in Table 4. Blank submission containers will be identical to the 4,000 mL submission containers used for general sample analysis for these parameter groups. All containers shall be pre-cleaned within the laboratory’s QA/QC program in the same manner as primary sample bottles for laboratory submission. Equipment blank results greater than the reporting limit for a parameter will be flagged for blank contamination. Field notes and sampling procedures will be reviewed to determine possible sources for contamination.

4.4.3

Composite Duplicate Sampling Method

Composite duplicate samples are samples collected in order to evaluate the sampling procedure and laboratory precision. Duplicate samples should theoretically represent the parameter(s) of interest at a given point in space and time equally. Duplicate samples are collected from the same sampling device and split into two separate submission containers. Duplicate samples will be submitted to the laboratory as blind or masked samples (see Table 5 for naming conventions). The true identity will be recorded in the field form.

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Table 5: Duplicate and Replicate Naming Conventions for CRWD Full Water Quality Sites.

Duplicate Alias

Replicate Alias

St. Anthony Park

Site

CRWD100

CRWD200

Hidden Falls Outlet

CRWD101

CRWD201

East Kittson

CRWD102

CRWD202

Phalen Creek

CRWD103

CRWD203

Trout Brook Outlet

CRWD104

CRWD204

Trout Brook-East Branch

CRWD105

CRWD205

Trout Brook-West Branch

CRWD106

CRWD206

Villa Park Outlet

CRWD107

CRWD207

Villa Park Inlet

CRWD108

CRWD208

Upper Villa Inlet

CRWD109

CRWD209

Como 3

CRWD110

CRWD210

Como 7

CRWD111

CRWD211

AHUG Inlet

CRWD112

CRWD212

TBNS-Rose

CRWD113

CRWD213

TBNS-Stream

CRWD114

CRWD214

MLK

CRWD115

CRWD215

Dickerman

CRWD116

CRWD216

Franklin

CRWD117

CRWD217

Field duplicate samples will be collected and submitted at the minimum 10% of total samples per year (approximately one per composite sampling trip).

4.4.4 Grab Replicate Sampling Method Grab replicates are defined as independent samples collected as close as possible to the same point in space and time. Grab sampling equipment is used to extract two separate samples from the same source. Replicate samples are stored in individual submission containers and are analyzed independently.

Field duplicate samples will be collected and submitted at the minimum frequency of 10% of samples throughout the sampling season (approximately one per grab sampling event). Replicate sample labels will be masked with an alias (Table 5) on both the submission container label and COC, with the true identity noted in the field forms.

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5.0 Quality Assurance Objectives The overall objectives of the CRWD QAPP is to develop and implement procedures for sample collection, laboratory analyses, and data reporting that will provide a high level of data. Six specific QAOs are defined below. This section provides goals to meet the QAO of precision, accuracy, representatives, completeness, comparability, and analytical sensitivity, along with the means by which they are measured. Table 6 lists all QA/QC methods employed by CRWD and the MCES laboratory to ensure that QAOs are being met.

Table 6: QA/QC Methods Employed to Reach QAOs. Sample Type

Description

Function

Method Blank

Reagent-grade water carried through sample preparation and analytical procedural method

To assess contamination from the laboratory preparation and analytical procedure

Laboratory Control Sample

Reagent-grade water spiked with known concentrations of analytes of interest

To determine the accuracy and consistency of instrument calibration

Quality Control Sample

A second source sample evaluated for analytes of interest

To verify purity and preparation of the calibration standards

Matrix Spike/Spike Duplicate

Separate aliquot of sample spike with known concentrations of the analytes

To determine the ability to recover the known analyte or compound in that sample matrix

Surrogate Spikes

Analytes, similar to those being evaluated, added at known concentration (organic analysis only)

To measure the performance of the analysis and to measure any matrix interferences

Internal Standards

Analyte, similar to those being evaluated, added to a sample at a constant concentration

To measure the relative responses of other method analytes and surrogates in the solution

Equipment Blank

Reagent-grade de-ionized water subject to sample collection, processing, and analysis

To evaluate contamination resulting from successive use of sampling equipment

Composite Duplicate

Duplicate of event sampling procedures

To estimate laboratory and sampling procedure precision

Grab Replicate

Two discrete samples taken concurrently from the same source

To estimate laboratory and sampling procedure precision

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5.1

Precision

Precision measures the reproducibility of measurements under a given set of conditions. Laboratory precision is determined by replicate analysis on a single sample, such as laboratory blank samples, laboratory spiked samples, field samples, or spiked field samples. Laboratory precision frequency is dictated by the laboratory’s QAM. Total precision, which combines both laboratory precision and field precision, is measured using masked field duplicate samples. Field duplicate samples provide precision information for the entire measurement system, including sample acquisition, handling, shipping, storage, preparation, and analysis.

Relative percent differences (%RPD) will be calculated for each pair of duplicate samples (laboratory replicates or field duplicate samples). When both values are reported as detected values, the following equation is used to determine precision:

% RPD =

Where:

S-D x 100 (S + D) / 2

S = First sample value D = Second sample value

CRWD upholds a target RPD value of 20%. If this threshold is exceeded, data will be flagged for that particular parameter.

23

5.2

Accuracy

Accuracy measures the bias in a measurement system. Laboratory accuracy is measured using the analytical results of matrix spike/matrix spike duplicates (MS/MSD), laboratory control samples/laboratory control sample duplicates (LCS/LCSD), as well as by instrument and method blank samples. To analyze MS/MSD samples, CRWD water samples are spiked with a known concentration of the analyte and compared against base levels in the original water sample.

LCS/LCSD utilizes the same process, but instead uses spiked pure water rather than sample water.

The percent recovery (%R) will be calculated using the following equation:

%R = Where:

A-B x 100 C

A = the analyte concentration determined from the spiked sample B = the native sample concentration of the unspiked sample C = the concentration of the spike added

Field accuracy is assessed conducting equipment blank samples. These samples are used to assess possible contamination in the sample collection procedures or sample bottle preparation processes. If samples are found to be contaminated, additional blank samples may be taken to identify the source of contamination.

CRWD upholds a target %R value of Âą20 when analyzing equipment blank samples. If this threshold is exceeded, data will be flagged for that particular parameter.

5.3

Completeness

Completeness is a measure of the amount of valid data obtained from a measurement system compared to the amount expected to be obtained under normal conditions. For laboratory completeness, it is expected that the contracted laboratory will provide useable and acceptable data for at least 95 percent of all samples collected using the specified analytical method.

Field completeness is calculated from the number of samples required by the city of Saint Paul’s permit and CRWD project goals in a calendar year. đ??śđ??śđ??śđ??śđ??śđ??śđ??śđ??śđ??śđ??śđ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’đ?‘’ =

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All baseflow and non-baseflow sites should have at least 12 storm samples submitted per year as well. The goal for baseflow sites is 20 samples per year. Continuously recorded level, velocity, and temperature data should be collected at 15-minute increments for the duration of the monitoring season, approximately 260 days. CRWD upholds a target field completeness goal of 95%.

5.4

Sensitivity

The achievement of reporting limits depends on instrument sensitivity and matrix effects. Instrument sensitivity will be monitored by the laboratory. It is expected that the laboratory will meet the sensitivities as required by the sample matrix and composition. To ensure that the analytical data are useful, the reporting limit for a given analyte should be well below the lowest expected ambient environmental concentrations or below any applicable regulatory action levels. The laboratory target reporting limits and methods of the monitored parameters are presented in Table 7. The actual reporting limits achieved may depend on available sample volume, sample matrix interferences, and target and non-target parameter concentrations.

25

Table 7: Laboratory Sample Parameters, Analytical Method, and Holding Times.

Parameter Ammonia Nitrogen

Hold Time

Method Detection Limit 0.005 mg/L

Reporting Limit 0.06 mg/L

1.1

0.2 mg/L

0.2 mg/L

Analytical Method

28 Days

EPA 350.1 Rev 2.0 SM 5210 B-2001, Hach 10360 Rev.

BOD – 5 Day Carbonaceous

48 Hours

Cadmium

6 Months

EPA 200.8, Rev. 5.4

0.0002 mg/L

0.0005 mg/L

Chloride

28 Days

SM 4500-Cl-E-1997

0.5 mg/L

2 mg/L

Chromium

6 Months

EPA 200.8, Rev. 5.4

0.00008 mg/L

0.00016 mg/L

Copper

6 Months

EPA 200.8, Rev. 5.4

0.0003 mg/L

0.0006 mg/L

SM 9223B-1997 (Colilert-18 Quanti

1 MPN/100

E. coli

6 Hours

Fluoride

28 Days

Hardness

28 Days

SM 2340 C-1997

N/A

5 mg/L

Lead

6 Months

EPA 200.8, Rev. 5.4

0.0001 mg/L

0.0005 mg/L

Nickel

6 Months

EPA 200.8, Rev. 5.4

0.0003 mg/L

0.0006 mg/L

Nitrate

28 Days

SM 4500-NO3 H-2000

0.01 mg/L

0.05 mg/L

Nitrite ORTHOPhosphate Potassium

28 Days

USGS I-4540-85A

0.003 mg/L

0.03 mg/L

48 Hours

SM 4500-P E

0.005 mg/L

0.01 mg/L

180 Days

EPA 200.8, Rev. 5.4

0.03 mg/L

1 mg/L

28 Days

EPA 300.0 Rev. 2.1

0.2 mg/L

0.5 mg/L

Sulfate Surfactants Total Dissolved Solids Total Kjeldahl Nitrogen Total Phosphorus Total Suspended Solids Volatile Suspended Solids Zinc

5.5

Tray)

N/A

mL

0.02 mg/L

48 Hours

0.10 mg/L

7 Days

SM 2540 C-1997

28 Days

5 mg/L

10 mg/L

EPA 351.2 Rev 2.0

0.03 mg/L

0.1 mg/L

28 Days

EPA 365.4, 1974

0.02 mg/L

0.05 mg/L

7 Days

SM 2540E-1997

1 mg/L

3 mg/L

7 Days

SM 2540E-1997

1 mg/L

2 mg/L

6 Months

EPA 200.8, Rev. 5.4

0.0008 mg/L

0.0016 mg/L

Comparability

Data comparability is the confidence with which one set of data from a particular site can be compared with another set from the same site. CRWD aims to use consistent field and laboratory methods for all sites from year to year, except where improvements are required for data quality.

Comparability will be evaluated by documenting that the sampling plan is followed or whether any 26

deviations from the plan have been made. It will also be evaluated by comparing analytical results from QA/QC samples, such as matrix spikes, equipment blanks, field blanks, method blanks, field duplicates.

5.6

Representativeness

Representativeness expresses the degree to which a sample or analytical result accurately and precisely represents a characteristic of a population, parameter variations at a sampling point, a process condition, or an environmental condition. Representativeness is a qualitative parameter that is dependent upon the proper design of the sampling program and proper laboratory protocol.

In the case of the monitoring program, representativeness is assessed with respect to the watershed pollutant concentrations and loads. Pollutant concentrations and loads differ with space, time and flow conditions. Spatial representativeness is assured by monitoring multiple subwatersheds within the district. It is also assured at baseline sites by taking samples as close to the subwatershed outlet as possible, making the sample more representative of the entire subwatershed. To assure samples are representative with respect to time and flow conditions, samples are taken throughout the year across varying flow conditions. Storm composite samples should collect both the rising and recession limbs of the hydrograph. Representativeness will be assessed by the analysis of the field duplicate samples.

6.0 Field and Sample Custody Documentation It is vital to record notes on field conditions such as weather, deviations from written standard operating procedures, equipment condition, and other unusual conditions. Understanding the sample’s path from collection to analysis may address issues which may arise, such as sample contamination. Thus, field documentation is essential to assure data quality for the CRWD monitoring program.

6.1

Field Forms

Field forms are the primary means for documenting field activities of staff. Electronic field forms are filled out at each site visit and all sampling events. Field forms are created in Google Forms, and are stored in a cloud server. Field forms are backed up onto the CRWD server on a weekly basis.

The field form contains the following information: 27

•

Date and time of site visit

•

List of field personnel present

•

Site name and activity

•

Instantaneous level, velocity, and discharge readings

•

Check boxes: system downloaded, site data checked, and desiccant changed

•

Sampler battery and programming information

•

For each composite sample:

•

-

Type of composite

-

Composite sample start date/time and end date/time

-

Number of samples, volume and bottle numbers filled

For each grab sample: -

Grab sample date/time

-

Number of grab samples

-

Type of grab sample(s)

•

Maintenance performed on-site

•

Notes

A quality control field form is also used to document information related to a duplicate, replicate, or blank sampling event. It contains fields to designate the type of quality control sample taken, the time and date a sample was taken, the alias given to the sample, and a field to make any notes. The quality control field forms are created in Google Forms and the response data is stored in a cloud server. The quality control field forms are backed up on the CRWD server on a weekly basis.

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6.2

Analytical Data Review

Laboratory data is available to CRWD from MCES through the MCES online data portal on a continuous basis. Private laboratories send analytical data in an automated electronic report after analysis. CRWD staff review the data upon receipt. If any analytical data are initially flagged as outliers or otherwise erroneous, they are compared against the field form for that sampling event and historic sampling records. The laboratory reports are reviewed and processed in preparation of the data editing, data review, and compilation of the annual report.

6.3

Sample Identification

Water samples will be identified by the location. Containers are typically labeled with the site name prior to use. Each laboratory submission container will be labeled with the following information using a waterproof marker on firmly affixed, water-resistant labels: •

Sample location

•

Sample collection date(s)

•

Sample collection time(s)

•

Initials of person collecting sample

•

Parameter names/groups to be analyzed (Private Lab only)

•

Preservation method (Private Lab only)

QA/QC samples will be identified with aliases denoted in the “Equipment Blank SOP” and “Duplicate/Replicate SOP” found in Appendix B. The aliases will also be identified in the Quality Control field form.

6.4

Chain of Custody

CRWD staff will begin filling out the COC in the field as samples are collected. The COC will be finalized by the Monitoring Coordinator during the compositing process. CRWD will make a copy of the completed COC upon arrival to MCES. An example of the COC is provided in Appendix C. All COC signatures related to sample custody will be made in ink. When sample custody is transferred to MCES lab staff, a signature, date and time will be entered at the time of transfer to document the transfer. A sample will be considered to be in custody if it is in any one of the following states:

29

•

In actual physical possession.

•

In view, after being in physical possession.

•

In physical possession and locked up so that no one can tamper with it.

•

In a secured area, restricted to authorized personnel.

A secured area such as a locked storage shed or locked vehicle may be used for temporary storage.

When using such an area, the time, date, and location of the secured area will be recorded on the COC next to the relinquisher’s signature. The time at which an individual regains custody will then be recorded in the “received by” space.

6.5

Sample Handling and Transport

CRWD staff accompany coolers containing samples from either the field of CRWD office to the MCES lab for analysis. CRWD staff will also transport a completed chain of custody form to the MCES lab. The samples will be kept at approximately 4 degrees Celsius during transport to laboratories. Before transporting samples, field personnel will perform the following tasks: 1. Verify that laboratory personnel will be present to receive the samples when they arrive.

2. Check labeling and documentation to ensure sample identity will be clear to laboratory personnel.

3. Hand deliver or ship samples in a manner that ensures samples will remain cool (about 4 degrees Celsius) until received by laboratory personnel.

4. Maintain possession of the samples and chain of custody, or note the current possessor.

5. Verify that laboratory personnel have received samples in good order and understand COC.

7.0 Quality Assurance Procedures 7.1

Laboratory Quality Assurance Procedures

MCES laboratory has a written comprehensive QA/QC program which provides rules and guidelines to ensure the reliability and validity of work conducted at the laboratory. Compliance with the QA/QC program is coordinated and monitored by the laboratory’s QA department, which is independent of the operating departments. The quality assurance officer shall do routine checks of the analytical procedures to ensure that the laboratory technicians are following the laboratory’s SOPs. 30

The laboratory’s SOPs shall reflect the methods and procedures for measuring, calibrating, and maintaining equipment outlined by the equipment’s manufacturer and analytical methodologies (EPA, Standard Methods, ASTM, etc.) specified in Table 7. The laboratory shall utilize the following quality control checks: sample spikes, surrogate spikes, reference samples controls, method blanks, instrument blanks, and laboratory duplicates of field samples. The laboratory shall specify the frequency, the compounds to be used for sample spikes and surrogate spikes, and the QA acceptance criteria for these checks.

The laboratory will flag any data sent to CRWD that do not meet instrument and analytical QA procedures. Any samples analyzed and determined to be in nonconformance with the QA criteria will be reanalyzed by the laboratory, if sufficient sample volume is available and sample is within holding time.

7.2 CRWD Quality Assurance Procedures All laboratory and continuous monitoring data collected through the CRWD monitoring program are reviewed by CRWD staff for quality before data analysis and inclusion in the annual report. This section provides an overview of the data and protocol review procedures for the monitoring program.

Most data management and data review happens within Kisters WISKI (WISKI). WISKI is a data management software specifically designed for continuous and discrete water quality data.

7.2.1

Data Management

The CRWD stormwater program manages two types of data: flow data collected by in-situ flow loggers and water quality data from samples submitted to the MCES laboratory. SOPs have been created for both data management tracks to ensure consistent and accurate data management and transfer between devices, programs, and databases.

7.2.1.1 Flow Data Management

Flow data is downloaded from field instruments regularly, either during water quality sample collection or on routine site visits. FlowLink 5 software is used to download data stored on the insitu data logger to a CRWD field laptop. Once stored on the laptop, automated backup schedules are run for each site to export the data from FlowLink to the laptop drive. Once on the laptop drive, a file synchronization software is used to export data from the laptop to the WISKI database. Site name labels within FlowLink must match labels within WISKI in order for the WISKI KIDAT software to import the data in the appropriate time series. Once the data is in the WISKI database

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and appropriate time series, all further changes and edits to the data will be logged within the program. Data is synced from the CRWD field laptops to the WISKI database on weekly basis.

7.2.1.2 Water Quality Data Management

Water quality data from the MCES lab is exported from the MCES database on a monthly basis.

Data for a specified time period is retrieved from the MCES online portal and saved to the CRWD server. The downloaded output is reformatted to match the requirements for the WISKI database importer. The WISKI importer scans the data for any potential errors for the user to review prior to importing. Once imported, the data is saved on the WISKI server and all further changes and edits to the data will be logged within the program.

7.2.2

Data Review and Validation

CRWD staff reviews, flags, and approves laboratory and continuous monitoring data for a given calendar year. SOPs dictate the proper protocol for reviewing and editing laboratory and continuously monitored data. The data review process draws upon the review of a number of QA/QC methods described in this QAPP including, but not limited to: •

Laboratory QC data (e.g. instrument and method blanks, spikes and calibration data)

•

CRWD QA/QC samples (e.g. equipment blanks, field blanks, and field duplicate samples)

•

Field notes and metadata to determine problems or deviations from written SOPs

All data review and validation occurs within the WISKI software. Any edits or changes to data will require confirmation by the user. WISKI will prompt the user to assign a quality code and a standard remark to data that has been edited in order to prevent loss and unintended edits. All edits can be reviewed and reversed within WISKI. 7.2.2.1 Continuous Flow Data Editing

Flow data is edited on a weekly basis. CRWD staff makes all edits to the data directly within the program using a set of quality codes and standard remarks in order to maintain consistent data processing (Table 8). Additionally, all the data editing documentation will be stored in one location for future re-review if necessary. Quality codes are saved within the program and can be used to remove a level of data quality from an analysis. Standard remarks are standardized notes that can be assigned to a section of data to describe how it was modified.

32

The goal of reviewing continuous flow data is to calculate pollutant loading by applying pollutant concentrations measured in the laboratory to discharge measurements. The continuous flow data editing process consists of the following steps: •

Compiling data for the entire year in WISKI software

•

Editing and reviewing annual data to account for missing or poor data points (Table 8): o

Assigning quality codes to data (e.g. Excellent, Good, Fair, Poor, Suspect, Unknown, Missing), or flagging bad data periods

o

Linearly interpolating between points where data is lost

o

Shifting data points where level has drifted

o

Recreating storm events using rating curves derived from the historical period of record

•

Separating baseflow from event flow using a script developed by CRWD staff in WISKI software. The script considers the following four discharge variables: o

Rate of change of slope on hydrograph—to identify event start

o

Rate of change in minutes—to identify the time span that change in the hydrograph has to occur in

o

Percent of flow—the percent of baseflow that must be reached before the event can stop (based on the time period preceding the storm)

o

Max storm duration—the maximum amount of time that event can last

33

Table 8: WISKI Quality Codes and Standard Remarks.

Code Excellent Good Fair Suspect Poor Unknown Missing

Number 0 40 80 120 160 200 255

Meaning Highest quality data - generally don't use this code Default for data that has been deemed OK after QA/QC process (unmodified) Any data that has been autocorrected, offset, slightly modified etc.

Data recreated from regression relationship Bad data. Needs recreation or deletion Default for all imported data - signifies that no QA/QC has been done Missing Data

WISKI Standard Remark

Previous CRWD Editing Description

Values copied

N/A

Values copied and proportionally fit to both sides

N/A

Range filled with constant

Set to constant

Range filled with linear interpretation

Autocorrected - bad/negative value

Gap filled with constant

Set to constant

Gap filled with linear interpretation

Autocorrected - missing

Gap inserted

Set to zero

Manually edited

Additional edit, add free comment

Manually inserted

Additional edit, add free comment

Range vertically shifted with constant

Fixed offset

Range vertically shifted by linear transformation

Proportional offset for level drift

Spikes removed

Autocorrect noisy data

WISKI Color Code

CRWD scans the data to look for missing periods, negative values, bad levels during storm, level drifts, and abnormal level increase. Issues with lower levels of complexity can be resolved with single data point editing. More complicated issues on a week or month timescale can be edited on a section basis. Table 9 indicates the most common data editing scenarios encountered in the continuous flow database.

34

Table 9: Steps for Various Data Editing Scenarios.

Quality Code Standard Remark

Problem with Data

Edit

Site does not have baseflow but contains standing water in pipe (where Q depends on L, i.e.: CCLRT sites)

Add validator in the Edited Level Time Series for the site and select KiScript script corrector N/A (choose Velocity Cut-off and base the cut-off on the Raw time series and the appropriate level)

N/A

There is data shown that occurs before install/after uninstall

Insert Data Gap

Missing

N/A

Missing data

None - leave the data as missing

Missing

N/A

Negative values during baseflow/non-storm periods

Edit the data using linear interpolation

Fair

Range filled with linear interpolation

Noisy data during baseflow/non-storm periods

Edit the data using the "Edit Values" icon and Fair manually reset data points of selected points or

Manually edited, free comment ("Removed noise")

Bad level data during storm and/or non-storm event

Insert Data Gap

Missing

Free comment (e.g.:"Sensor ripped out")

Abnormal level values between two dates: - Clear start/stop points where level goes bad, i.e. as what happens between poor level cals where the first is off and the second corrects it so that a section of data is all off by the same (or relatively the same) amount.

- Unclear start/stop points where level goes

Edit the data using the Shift range vertically option. Use either Parallel shift or Linear transformation depending on what the data looks like.

Fair

Range vertically shifted with constant x.xx OR Range vertically shifted by linear transformation

bad, i.e. the start of a section of data appears good, and then the level drifts down until there is a point where the data appears good again.

Edit the data using Drift Correction (1st data point should be previous known valid point. 2nd is the data point where drift was corrected by Fair field calibration (Isco equipment) or the field observed reading (Global water level loggers)

Manually edited

7.2.2.2 Water Quality Data Editing

Water quality data is subject to visual review upon retrieval from the MCES online portal. This review serves as a preliminary screening to identify any blatant outliers. The data is then imported into WISKI using a pre-configured importer, which scans the water quality data and prompts the user to review errors found in the program (e.g. missing units).

Upon completion of the monitoring season, CRWD staff conduct a second review to identify outliers in the water quality dataset in WISKI. This review evaluates the TP and TSS parameters and their comparison to other nutrient parameters. CRWD staff identified thresholds of 1.5 mg/L of TP and 2000 mg/L of TSS as boundaries above which data would be of concerning quality. These thresholds were informed by reviewing data in the historical period of reference. Samples exceeding these thresholds will be noted with the date and time. These samples will be plotted with the TKN and Ortho-P parameters to provide a context for whether or not a data point could be plausible. If the elevated TP and/or TSS values fit in context in relation to TKN and Ortho-P values, then the value is determined to be valid. If the high TP and/or TSS value is not explained in context of other parameters, the data point for all parameters is flagged as an erroneous data point and assigned a

35

quality code of “Poor.� Field form records are also used to determine whether or not sampling procedure, field conditions, or other factors could provide context for the exceeded TP or TSS value.

7.3

Corrective Action

Corrective action for this program is the responsibility of CRWD during both the data management and data review processes. Corrective action will be implemented if it is determined that the data generated does not fulfill the program objectives.

When the QC data exceed the acceptance criteria, corrective actions may be implemented. Possible problems requiring corrective action include: 1. Sample contamination 2. Equipment malfunction 3. Non-compliance with quality control system 4. Errors in sampling procedure 5. Laboratory error

8.0 Data Assessment and Reporting Data assessment and reporting are two oversight elements that ensure that the QA Project Plan is implemented as prescribed in this document. Additionally, annual data reporting is the primary method of providing stakeholders in the CRWD monitoring program with monitoring results.

8.1

Internal Quality Assessments

The Program Manager, or designee, shall review the procedures used by the monitoring program periodically, or at least once per year. This review will ensure written procedures remain consistent, clear, and current. During this review, the reviewer shall participate in field activities to ensure that field staff is following established SOPs, that deviations from the SOPs are documented, and that field documentation is complete and accurate. Data flagged by MCES for not meeting QA requirements shall also be revaluated, as described in Section 7.2.2.1. Data will also be analyzed for patterns that may reveal possible problems with monitoring procedures, or monitoring gaps.

36

8.2

Electronic Data Reporting

CRWD submits its stormwater data to the MPCA Environmental Quality Information System (EQuIS) database for the majority of tested parameters. EQuIS is a resource that contains waterrelated monitoring data and associated laboratory results from statewide monitoring locations.

8.3

Annual Monitoring Report

Data collected from the monitoring program are compiled and analyzed for the Annual Stormwater Monitoring Report. The report is prepared by CRWD staff and approved by the CRWD Board and CAC. The report is distributed to the cities of Saint Paul, Roseville, Falcon Heights, Lauderdale, and Maplewood and is available to public electronically on the CRWD website. The report follows the following general outline: •

Chapter 1

Executive Summary

•

Chapter 2

Introduction

•

Chapter 3

Methods

•

Chapter 4

Climatological Summary

•

Chapter 5

All Sites Results Summary

•

Chapter 6-12 Sub-Watershed Chapters

•

Chapter 13

•

References

•

Appendices

Conclusions

37

9.0 References City of Gresham, Department of Environmental Services, Watershed Division. 2012. Stormwater Monitoring and Quality Assurance Plan. Gresham, OR Fisher, Roger. 2007. MWMO Ambient Surface Water Monitoring Quality Assurance Program Plan.

Minnesota Pollution Control Agency. Report Prepared for the Mississippi Watershed Management Organization, Minneapolis, MN Minneapolis Park and Recreation Board: Environmental Stewardship, Water Resource Management.

2015. 2013 Water Resources Report. Minneapolis, MN Minnehaha Creek Watershed District. 2015. Water Quality Technical Report – 2014. Minnetonka, MN Minnesota Pollution Control Agency. 2016. National Pollutant Discharge Elimination System. Permit No. MN 0061263. St. Paul, MN Metropolitan Council Environmental Services Environmental: Monitoring and Assessment Section, Water Resources Assessment Section. 2011. Quality Assurance Program Plan: Stream Monitoring.

Saint Paul, MN Mitchell, Patricia. 2006. Guidelines for Quality Assurance and Quality Control in Surface Water Quality Programs in Alberta. Patricia Mitchell Environmental Consulting. Report Prepared for Alberta Environment. Edmonton, Alberta Teledyne Isco. 2012a. Isco 2150 Area Velocity Flow Module and Sensor Installation and Operation Guide, Revision DD. Lincoln, NE. http://www.isco.com/manuals/UP0018D2.pdf Teledyne Isco. 2012b. Isco 2105 Interface Module Installation and Operation Guide, Revision L. Lincoln, NE. http://isco.com/manuals/UP00194J.pdf Teledyne Isco. 2013. Isco 750 Area Velocity Module Installation and Operation Guide, Revision L. Lincoln, NE. http://www.isco.com/manuals/UP001AU8.pdf Teledyne Isco. 2015. Isco 6712 Portable Samplers Installation and Operation Guide, Revision EE. Lincoln, NE. http://www.isco.com/manuals/UP001DT6.pdf US EPA. 2001. EPA Requirements for Quality Assurance Project Plans. EPA QA/R-5. Washington, DC

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Appendix A CRWD Organizational Chart

39

40

Figure A-1: CRWD Organizational Chart Citizens

Ramsey County Commissioners

CAC

Board of Managers

Administrator

Engineer

Program Manager

Program Manager

Program Manager

Monitoring and Research

Regulatory Program

Planning, Projects and Grants

Monitoring Coordinator

Water Resource Technician Water Resource Technician

BMP Inspector

Attorney

Community Outreach Coordinator

Water Resource Project Manager

Water Resource Specialist

Water Resource Technician Water Resoure Technician (Seasonal)

41

Outreach Specialist

Office Manager

Administrative Assistant

42

Appendix B Field Standard Operating Procedures • Sample Compositing • ISCO Sampler Bottle Cleaning • Base Grab Sampling • Storm Grab Sampling • Base Composite Sampling • Storm Composite Sampling • ISCO 6712 Volume Calibration • ISCO 2150 Level Calibration • Importing Logger Data into WISKI • Importing SW Lab Data into WISKI • Outlier Identification

43

44

5B. Sample Compositing Purpose: To obtain an accurate reading of the parameter concentrations based on the amount of water obtained in the ISCO sample bottles.

General: When an ISCO sampler is set to sample for a base composite or storm composite, the flow will be sampled over a period of time in order to identify the concentrations of certain parameters over that period. Instead of getting one snapshot of an event, the sampler will collect the flow based on the volume of the water that passes through or based on the amount of time that passes. Once the carousel containing this water is collected, the water must be combined in such a way that allows each bottle to be equally represented. The water at the beginning of a storm generally has a high concentration of sediment, nutrients, etc. that could skew the sample if more of this bottle was added than a later bottle. Equal representation of each sample bottle is achieved by adding a predetermined volume from the ISCO bottle to the churns and then mixing the samples together and dispensing the sample into the labeled sample bottles.

Frequency: After a sampling event when a composite sample is collected Number of Staff: 2 Expected Time for Completion: ½ Day Equipment: • • • • • • • •

1000 mL Graduated cylinder Filled Carousels 4000 mL sample container(s) 8000 mL churn 14000 milliliter churn Sample Labels Safety Glasses Safety Gloves

Volumes: MCES Sample Bottles: • Sample Bottle = 4,000 mL (4 L) • Minimum volume for all parameters = 2,160 mL 171

Churns: • Small = 8,000 mL (8 L) • Large = 14,000 mL (14 L) Compact Sized Sampler: • 1 bottle = 400 mL (2- 200 mL discrete samples) • Full carousel (24 bottles) = 9,600 mL Full Sized Sampler: • 1 bottle = 800 mL (4- 200 mL discrete samples) • Full carousel (24 bottles) = 19,200 mL

Compositing Procedures 1. Look up the event details in the field form.

2. Follow the instructions below based on the applicable scenario: Scenario 1: Storm event volume is <4,000 mL • • •

No churning required Vigorously shake carousel sample bottles to agitate settled out solids Dump each individual bottle directly into the MCES sample bottle

Scenario 2: Compact carousel is filled, or total volume is >4,000 mL • • •

Vigorously shake carousel sample bottles to agitate settled out solids and dump all contents into the large 14 L churn o If total volume is less than 8 L, the small churn can be used instead Churn and mix all samples Using nozzle, fill MCES sample bottle while continuing to churn sample

Scenario 3: Full-sized carousel is filled, or total volume is >4,000 mL •

If total volume of carousel bottles is less than 14 L: o Vigorously shake carousel sample bottles to agitate settled out solids and dump contents of each individual bottle into the large 14 L churn o Churn and mix all samples o Using nozzle, fill MCES sample bottle while continuing to churn sample 172

•

If total volume of carousel bottles is greater than 14 L: o Count the total number of carousel bottles and divide it into two sample sets o For the first sample set:  Vigorously shake carousel sample bottles to agitate settled out solids and dump contents of each individual bottle into the large 14 L churn  Churn and mix all samples  Using nozzle, fill MCES sample bottle HALF WAY (2,000 mL) while continuing to churn sample  Discard remaining water in churn by dumping it in the sink o For second sample set:  Repeat above

3. Label each bottle based on what is found in Flowlink.

4. Repeat the process for all stations.

Churn Cleaning Procedures 1. Rinse the churn three times with tap water between compositing samples from different stations.

2. When all stations have been composited, scrub all churn components with Liquinox soap and rinse thoroughly. Place churn and components on the bottom rack of the dishwasher to be sterilized (the top rack of the dishwasher will need to be removed), and set to “Pots & Pans” setting. Put equipment back on shelves once finished.

173

174

8B. ISCO Sampler Bottle Cleaning Purpose: To ensure that the sampler bottles are properly cleaned and ready for use in the field.

General: When sampler bottles are returned to the shop for cleaning, it is important to wash them right away to prevent sediment from sticking to inside of bottles. Two staff members can work on this procedure at the same time. The sample bottles are first rinsed with tap water to remove floating sediment. The sink is filled with warm, soapy water and bottles are scrubbed with the scrub brushes to remove any visible dirt. Following the scrub, the bottles are loaded into the dishwasher on the heaviest duty setting. Once the bottles are finished, they can be reloaded into the carousels.

Frequency: Each time bottles are returned from the field Number of Staff: 2 Expected Time for Completion: Half of day Equipment: • • • • •

Sample bottles Scrub Brushes Liquinox Dishwasher Sink

Procedure: 1. Rinse inside of bottles with tap water to loosen any sediment in the bottom of the bottles and rinse down sink.

2. Fill sink with rinsed bottles.

3. Fill shop sink with warm water and liquinox soap.

4. Bottle caps can be loaded into dishwasher baskets. Only load to ¾ full. Baskets can then be dunked in the water/liquinox and thoroughly churned up and down. When finished, rinse through the baskets using the sprayer nozzle on the sink to thoroughly remove soap from the caps. Place baskets inside of bottom rack of dishwasher.

215

5. Use brushes to scrub the inside of the bottles, removing any caked on dirt and sediment.

6. Rinse remaining suds with water from faucet.

7. Place 1000 milliliter bottles in bottom rack of dishwasher and 500 milliliter bottles on top rack of dishwasher.

8. Do NOT use dishwashing detergent or any other soap in the dishwashers to wash the bottles.

9. Select “Pots and Pans” and run. If unable to run dishwasher right away, move paper on outside of dishwasher to “DIRTY” so that others know the status of the bottles.

10. When dishwasher cycle is complete, remove and inspect bottles. If unable to remove bottles right when cycle is finished, move paper on outside of dishwasher to “CLEAN” so that others know the status of the bottles.

11. Set aside bottles that require additional cleaning.

12. Rerun the dirty bottles.

13. Place clean bottles in a clean sampler carousel and strap in circular bottle holder with black bungees.

14. Place carousel onto the appropriate shelf. Only CLEAN bottles and carousels should be placed on the shelf.

15. Bottle caps are air dried and then placed in the 4L bottles by size for storage. If caps need to be stored and/or used quickly, dry on top of floor fan that is placed up on top of a table. Do not put wet caps inside the sealed cap bottles.

16. If cap bottles appear to have condensation inside as a result of wet bottle caps, remove top on bottle and allow inside to air out.

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2C. Base Grab Sampling (Field Season) Purpose: To collect water for lab analysis under base-flow conditions, when base compositing fails.

General: The base grab procedures can be completed at all stations that flow during non-storm conditions. Follow the “Base Grab (Winter) SOP” when samplers are removed for the season.

The BMPs and other stations are dry during non-storm events, so no base sampling occurs. At each sampled station, both a Full Water Quality sample and an E. coli sample are obtained, the station data is downloaded and checked, and regular station maintenance is performed.

Frequency: During monitoring season, take base composite or grab samples twice a month. A base grab is also completed if a base composite fails (make sure that it has not rained within two days of collecting base samples).

Locations: All stations with baseflow Number of Staff: 2 Expected Time for Completion: One day for baseline stations including lab preparation, bottle washing, and shop cleanup Equipment: •

Clipboard with: o Sharpies o Pens o Base grab lab sheet (Met Council)

•

Backpack with: o Isco Sampler connection cords o Ziplock bag containing charged desiccant o Ziplock bag for spent desiccant o Voltmeter o Extra pump tubes

•

Sampling Equipment: o E. coli grab sampler (bring both) o 4000 mL grab sampler and rope (bring both) 25

o 4000 mL bottles labeled for each station  With 2 extra bottles o Whirlpaks labeled E. coli for each station  3-4 extra whirlpaks o Large coolers (for 4000 mL samples) o Small coolers with icepacks (for E. coli samples) o T-wrench for vault doors (TBEB, TBWB, TBO) o Manhole hook (for Phalen ) •

Record Maintenance Equipment: o Laptop o iPad o Waterproof camera

•

Miscellaneous items: o Marine batteries (one for each station) o 6V batteries (for 2150 flow loggers) o Tool box o Lock key (in truck glove box) o Hand sanitizer (in truck glove box) o Safety vests if sampling in street o One large and one small carousel of bottles

Procedure: Before Leaving the Shop: 1. If samples are to be submitted to the Met Council, call the Met Council lab in the morning before sampling at (651) 602-8293. E. coli samples only have a 4 hour hold time, so plan appropriately. Leave a message or advise the Met Council staff of: • • •

The number of full water quality samples to be submitted The number of E. coli samples to be submitted The approximate time of arrival to the Met Council (E. coli samples must be dropped off no later than 2pm. Full water quality samples can be dropped off a little later in the day because they can be processed the next day. On Fridays, however, all samples need to be turned in as soon as possible as processing needs to occur that day.)

26

2. Each of the two staff members will check over the equipment list before leaving the office.

Downloading: 1. One staff member will connect using the laptop computer and Isco connection cable to the 2150 Flow logger.

2. Once connected, download the data using Flowlink. Downloading takes a few minutes, continue with procedures until downloading is completed.

3. Record the level, velocity, and calculated discharge from Flowlink into the Ipad field form.

4. Using the voltmeter, connect to the marine battery terminals. Record the marine battery charge and current battery ID. If the battery charge is below 70% or 12.3V, replace the battery once you are no longer “connected” in Flowlink. For those stations that have marine battery power for the 2150, also replace the battery if it reaches 12.3V. 5. If the 2150 is powered by 6V batteries, check the internal battery charge of the Isco 2150 Flow Logger by going to the “Measurements” tab in Flowlink. Record the internal battery voltage in the field form. If the voltage is below 10.2V, replace the 6V batteries inside the Isco 2150 Flow Logger once Flowlink is disconnected.

6. For stations with 2150 flow loggers, check the desiccant of the Isco 2150 Flow Logger and Isco 2105 interface module. If the desiccant is blue or orange, the desiccant does not need to be replaced. If the desiccant is pink or green, replace the desiccant.

7. Once the data is finished downloading, check over the data. Record any missing data or abnormalities in flow in the field form. Also check the data to ensure flow levels correspond to baseflow conditions, and note any abnormalities in the field form.

Disconnect from Flowlink.

8. Disconnect the cord connecting the laptop to the sampler or 2150. Ensure all protective connector covers are secured.

9. Check the bottles inside the sampler to ensure no samples were taken. Replace any dirty bottles and restart the program if needed.

27

Sampling: 1. The second staff member will record the sample date and time on the sample bottles, whirlpak, and field form. Ensure all sample dates and times are the same for all three.

2. Gain access to the sampling area. Open the manhole using the manhole hook or open the vault doors using a T-wrench if needed. â&#x20AC;˘ â&#x20AC;˘ â&#x20AC;˘

At Villa Park Inlet, remove the grate over the drain and collect the sample from the water overflowing the drain.

At Villa Park Outlet, reach down between the grate and collect the sample, trying not to let the bag touch the bottom of the culvert.

At Kittson, use the manual pump function on the 6712. Disconnect the pump tubing at the location where it enters the sampler bottle housing. Using the keypad on the Isco 6712 Sampler, pump water forward until water is flowing from the tube. Allow the water to flow through the tube for at least 30 seconds to rinse the line. Pump water directly into the E. coli whirlpak, then place in cooler. Pump water backwards for approximately one minute to purge the sample line. Reconnect the pump tubing to the bottle housing on the sampler when completed.

3. Lower the large 4000 mL sampler using a rope into the water. Lift the sampler with the water back to the surface, swirl the water inside the sampler, and dump the water outside of the sample area. Repeat this step twice for a total of three rinses of the sampler.

4. Lower the large 4000 mL sampler into the water to retrieve a water sample. Pour the water into each of the large sample bottles needed for the station. Secure the sample bottle lids and place the samples in the large coolers.

5. Attach the appropriate E. coli bag to the E. coli sampler. Ensure your fingers do not touch the inside of the whirlpak bag as this can contaminate the sample. Lower the E. coli bag into the water. Retrieve the sample. The water level inside of the bag should be above the white line on the whirlpak. If the water level is not above the white line, then try again. This can take several attempts. Once enough sample is retrieved, secure the whirlpak, check to make sure the bag is not leaking, and place inside a small cooler.

28

*At locations with high/fast flow, it is often difficult to fill the E. Coli bag to the desired level. At these locations, follow these steps. First, lower bag until it is submerged in top of water. Keeping bag at current position, let out slack from rope and hold on to it at the top in one hand. In one motion, release all the slack on the line at once to allow bag to fully submerge and be carried downstream. Proceed to retrieve sample.

6. Secure the manhole, vault door, or grate if needed.

Before Leaving the station: 1. Take pictures of any abnormalities in the field. This can include but is not limited to a noticed illicit discharge, abnormally dark water in sample bottles, or equipment damage.

2. Check to ensure the sampler states “Disabled” on the display screen, and the sampler is ready to sample starting at Bottle #1. Restart the program on the 6712 if needed.

3. Check to ensure the sampler is aligned correctly on the bottles, and all tubing is attached appropriately.

4. Ensure all of the caps are secured on back of the sampler or flow logger.

5. Check that the sample dates and times are the same on the sample bottles, lab sheets, and in field form.

6. Check the station for any remaining equipment.

7. Secure the lock on the sampler box. Repeat these procedures for each station.

Before Returning to the Shop: 1. E. coli samples only have a 4 hour hold time. Met Council samples must be taken immediately to the lab for analysis. Any full water quality samples to be analyzed by the Met Council can be dropped off at this time. Make a copy of the Lab Form when you arrive at the lab, leave one with the samples, and return the other to the office. Grab clean 4000 mL bottles from the lab if needed. (see “Metropolitan Council Lab Procedures SOP”)

29

At the Shop: 1. Unload, clean up, and put away all equipment.

2. Place any samples in the shop refrigerator.

3. Wash any dirty bottles (see â&#x20AC;&#x153;Isco Sampler Bottle Cleaning SOPâ&#x20AC;?).

4. Check over the field notes and sample times in the field form. Advise other monitoring staff of any problems that occurred in the field. Note any repairs needed on the white board in the hallway.

30

2E. Storm Grab Sampling (Field Season) Purpose: To collect water for lab analysis under stormflow conditions.

General: The storm grab procedures can be completed at all stations. At stations with a functioning Isco 6712 Sampler, only an E. coli sample is taken since a storm composite should be taken by the sampler. If the sampler is broken (or staff wants to ensure a sample is collected), then both an E. coli grab and a full water quality grab are taken at the station.

Samples are only collected if water is currently flowing in the street gutters. Since time is limited and equipment can be damaged by rainfall exposure, the stations are not downloaded.

Samples are collected and submitted to the lab.

Frequency: During the monitoring season, take storm grab (or composite samples) at least twice a month (dependent upon storm frequency and number) Locations: All BMP and Baseline stations. Additional stations (completed either during the rain event, or after a certain amount of time following a rain event) may include: William Street Pond, Upper Villa Exfiltration, and the GRS jellyfish. See individual SOPs for details.

Number of Staff: 2 teams of 2 Expected Time for Completion: Approximately one morning.

Equipment: •

Clipboard with: o Sharpies o Pens o Lab Sheet (Met Council)

•

Sampling Equipment: o E. coli grab sampler (bring both) o 4000 mL grab sampler and rope (bring both) o 4000 mL bottles labeled for each required station  With 2 extra bottles o Whirlpaks labeled E. coli for each station  3-4 extra whirlpaks o Large coolers (for 4000 mL samples) o Small coolers (for E. coli samples) 37

o T-wrench for vault doors (TBEB, TBWB, TBO) o Manhole hook (for Phalen) •

Record Maintenance Equipment: o Laptop o iPad o Waterproof camera

•

Miscellaneous items: o Latex gloves (some stations regularly have high E. coli counts) o Waterproof camera o Rain gear o Rubber boots o Tool box o Lock key (in truck glove box) o Hand sanitizer (in truck glove box) o Safety vests and cones if sampling in street o Umbrella

Procedure: Before Leaving the Shop: 1. Check the weather and radar maps to determine the best time to leave the office.

Create a prioritized list of stations to sample. On the list, determine if only an E. coli sample is needed or both E. coli and full water quality samples will be taken.

2. If samples are to be submitted to the Met Council, e-mail the Met Council lab in the morning before sampling. See monitoring coordinator for current list of e-mail addresses. Be sure to note: • • •

The estimated number of full water quality samples to be submitted The estimated number of E. coli samples to be submitted The approximate time of arrival to the Met Council (E. coli samples must be dropped off no later than 2pm. Full water quality samples can be dropped off a little later in the day because they can be processed the next day. On Fridays, however, all samples need to be turned in as soon as possible as processing needs to occur that day.)

38

3. Before leaving the office, each of the two members of the field team will check over the equipment in the truck.

Sampling: 1. Before collecting any sample at a station, make sure water is flowing in the street gutters. Do not take any samples if no water is flowing. If it is raining but no water is flowing in the gutters yet, then you can wait for water to start flowing. ***Stop sampling and return to the truck or office if lightning or severe weather is present!*** 2. Record the sample date and time on the sample bottles, whirlpak, and in the field form.

Ensure all sample dates and times are recorded the same in all three.

3. Gain access to the sampling station if needed. Open the manhole using the manhole hook or open the vault doors using a T-wrench if needed. (At Villa Park Outlet, staff member may need to lay on grate over culvert and reach between bars to collect the sample.) **For East Kittson, the road becomes unsafe and hard to drive on during/right after very heavy rains. If sampling during heavy rain, do not try to drive down the road. Park instead at the location for winter sampling (in the small residential parking lot on the west side of Lexington Ave just after the bridge over the railroad tracks).

4. If only an E. coli sample is needed at the station, skip to Step 6. If a full water quality sample is needed at the station, lower the large 4000 mL sampler using a rope into the water. Lift the sampler with the water back to the surface, swirl the water inside the sampler, and dump the water outside of the sample station. Repeat this step twice for a total of three rinses of the sampler. **For East Kittson, the water cannot be accessed by lowering the sampler into the manhole. Disconnect the pump tubing at the location where it enters the sampler bottle housing. Using the keypad on the Isco 6712 Sampler, pump water forward until water is flowing from the tube. Allow the water to flow through the tube for at least 30 seconds to rinse the line. Pump water directly into both the large 4000mL sample bottle and E. coli whirlpak. Secure both samples and place them in the appropriate coolers. Pump 39

water backwards for approximately one minute to purge the sample line. Reconnect the pump tubing to the bottle housing on the sampler. Continue with Step 7.

5. Lower the large 4000 mL sampler into the water to retrieve a water sample. Pour the water into the large sample bottle needed for the station. Secure the sample bottle lid and place the samples in the large coolers.

6. Attach the appropriate E. coli bag to the E. coli sampler. Ensure your fingers do not touch the inside of the whirlpak bag as this can contaminate the sample. Lower the E. coli bag into the water. Retrieve the sample. The water level inside of the bag should be above the white line on the whirlpak. If the water level is not above the white line, then try again. This can take several attempts. Once enough sample water is retrieved, secure the whirlpak, check to make sure the bag is not leaking, and place inside a small cooler. **At locations with high/fast flow, it is often difficult to fill the E. Coli bag to the desired level. At these locations, follow these steps. First, lower bag until it is submerged in top of water. Keeping bag at current position, let out slack from rope and hold on to it at the top in one hand. In one motion, release all the slack on the line at once to allow bag to fully submerge and be carried downstream. Proceed to retrieve sample.

7. Secure the manhole, vault door, or grate if needed.

Before Leaving the station: 1. Take pictures of any abnormalities in the field. This can include but is not limited to a noticed illicit discharge, abnormally dark water in sample bottles, or equipment damage.

2. Check that the sample dates and times are the same on the sample bottles, lab sheets, and in the field form.

3. Check the station for any remaining equipment.

4. Secure the lock on the sampler box. Repeat these procedures for each station.

Before Returning to the Shop: 1. E. coli samples only have a 4 hour hold time. Met Council samples must be taken immediately to the lab for analysis. Any full water quality samples to be analyzed by the Met Council can be dropped off at this time. Make a copy of the Lab Form, leave one 40

with the samples, and return the other to the office. Grab clean 4000mL bottles from the lab if needed. (see “Metropolitan Council Lab Procedures SOP”)

At the Shop: 1. Unload, clean up, and put away all equipment.

2. Place any samples in the shop refrigerator.

3. Wash any dirty bottles (see “Isco Sampler Bottle Cleaning SOP”).

4. Check over the field notes and sample times in the field form. Advise technicians of any problems that occurred in the field. Note any repairs needed on the white board in the hallway.

41

42

2B. Base Composite Sampling Purpose: To collect a representative series of base samples from Baseline stations for lab analysis General: The base composite procedures can be completed at all stations that flow during nonstorm conditions during the field season and have an installed Isco 6712 sampler. The BMP stations are dry during non-storm events, so no base sampling occurs. Base composites take a total of two days to complete. On the first day, the station data is downloaded and checked, an E. coli sample is taken, and the programming is changed to take samples at a set discharge interval over the next day. On the second day, the composite samples are retrieved, the data is downloaded and checked, and the programming is restored to sample for a storm event.

Frequency: During the monitoring season, two base composites or grabs are taken monthly (Make sure that it has not rained within two days of collecting composite samples, and there is no chance of rain during the 24 hrs that compositing will occur) Locations: All Baseline stations Number of Staff: 2 Expected Time for Completion: Two days including sample preparation, lab delivery, bottle washing, and shop clean-up Equipment: Day 1: â&#x20AC;˘

Clipboard with: o Sharpies o Pens o Lab Sheet (Met Council) o Pacing Sheet

â&#x20AC;˘

Backpack with: o Isco Sampler connection cords o Ziplock bag containing charged desiccant o Ziplock bag for spent desiccant o Voltmeter 15

•

Sampling Equipment: o E. coli grab sampler (bring both) o Whirlpaks labeled E. coli for each station  3-4 extra whirlpaks o Small coolers with icepacks (for E. coli samples) o T-wrench for vault doors (TBEB, TBWB, TBO) o Manhole hook (for Phalen)

•

Record Maintenance Equipment: o Laptop o iPad o Waterproof camera

•

Miscellaneous items: o Marine batteries (one for each station) o 6V batteries (for stations with 2150 flow loggers) o Tool box o Lock key (in truck glove box) o Hand Sanitizer (in truck glove box)

Day 2: •

Clipboard with: o Sharpies o Pens o Base grab lab sheet (Met Council)

•

Backpack with: o Isco Sampler connection cords o Ziplock bag containing charged desiccant  Blue or Orange desiccant for 2150 stations o Ziplock bag for spent desiccant o Voltmeter o Extra pump tubes

•

Sampling Equipment: o 4000 mL grab sampler and rope (bring both) o 3-4 extra 4000 mL bottles in large cooler o Containers of large and small caps 16

o o o o

4 Compact Carousels (SAP, Hidden Falls, Villa In, Villa Out) 5 Full Size Carousels (Kittson, Phalen, TBO, TBEB, TBWB) T-wrench for vault doors (TBO, TBEB, TBWB) Manhole hook (for Phalen)

•

Record Maintenance Equipment: o Laptop o Waterproof camera

•

Miscellaneous items: o Marine batteries (for all stations) o 6V batteries (for stations with 2150 flow loggers) o Tool box o Lock key (in truck glove box) o Hand Sanitizer

Procedure: Day 1: Before Leaving the Shop: 1. If E. coli samples are to be submitted to the Met Council for analysis, e-mail the Met Council lab in the morning before sampling. See monitoring coordinator for current list of e-mail addresses. Be sure to note: • •

The number of E. coli samples to be submitted The approximate time of arrival to the Met Council (E. coli samples must be dropped off no later than 2pm. Full water quality samples can be dropped off a little later in the day because they can be processed the next day. On Fridays, however, all samples need to be turned in as soon as possible as processing needs to occur that day.)

2. Both field team members will check over the equipment list before leaving the office.

17

Downloading and Changing the Programming: 1. At most stations, both an Isco 2150 Flow logger and an Isco 6712 Sampler are contained inside the box. One staff member will connect to the Isco 2150 Flow Logger while the second team member will begin with the sampling (see the Sampling section).

2. Once connected, download the data using Flowlink 5.1 (see “6712/750 Module Download SOP” or “2150 Module Download SOP” depending on the station).

Downloading takes a few minutes, continue with procedures until downloading is completed.

3. Record the level, velocity, and calculated discharge from Flowlink into the iPad field form.

4. Using the voltmeter, connect to the marine battery terminals. Record the marine battery charge and current battery ID. If the battery charge is below 70% or 12.3V, replace the battery once you are no longer “connected” in Flowlink. For those stations that have marine battery power for the 2150, also replace the battery if it reaches 12.3V. 5. If the 2150 is powered by 6V batteries, check the internal battery charge of the Isco 2150 Flow Logger by going to the “Measurements” tab in Flowlink. Record the internal battery voltage in the laptop field form. If the voltage is below 10.2V, replace the 6V batteries inside the Isco 2150 Flow Logger once Flowlink is disconnected.

6. For stations with 2150 flow loggers, check the desiccant of the Isco 2150 Flow Logger and Isco 2105 interface module. If the desiccant is blue or orange, the desiccant does not need to be replaced. If the desiccant is pink or green, replace the desiccant.

7. Once the data is finished downloading, click “disconnect” in Flowlink. Check the data in Flowlink (see “6712/750 Module Download” or “2150 Module Download”). Record any missing or abnormal data in the field form. Also check the data to ensure flow levels correspond to baseflow conditions, note any abnormalities in the field form.

8. Record the current pacing and trigger into the field form (See “Isco Instrument Pacing SOP”).

18

9. Take note of the current discharge (cfs) and the bottle size at the station (small or large).

Using a calculator, determine the new pacing by the following equation (note that the pacing value determined by the formula is divided by 10 at the end, because the 6712 is set up for 10 pulses per sample):

New Pacing =

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48 (đ?&#x2018;&#x201C;đ?&#x2018;&#x201C;đ?&#x2018;&#x201C;đ?&#x2018;&#x201C;đ?&#x2018;&#x201C;đ?&#x2018;&#x201C; đ?&#x2018; đ?&#x2018; đ?&#x2018; đ?&#x2018; đ?&#x2018; đ?&#x2018; đ?&#x2018; đ?&#x2018; đ?&#x2018; đ?&#x2018; đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?)đ?&#x2018;&#x153;đ?&#x2018;&#x153;đ?&#x2018;&#x153;đ?&#x2018;&#x153; 96 (đ?&#x2018;&#x201C;đ?&#x2018;&#x201C;đ?&#x2018;&#x201C;đ?&#x2018;&#x201C;đ?&#x2018;&#x201C;đ?&#x2018;&#x201C; đ?&#x2018;&#x2122;đ?&#x2018;&#x2122;đ?&#x2018;&#x2122;đ?&#x2018;&#x2122;đ?&#x2018;&#x2122;đ?&#x2018;&#x2122;đ?&#x2018;&#x2122;đ?&#x2018;&#x2122;đ?&#x2018;&#x2122;đ?&#x2018;&#x2122; đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?đ?&#x2018;?)

/10 pulses

10. Program the new calculated pacing value into Flowlink and click apply. Change the sampler enable to â&#x20AC;&#x153;Always.â&#x20AC;?

11. Apply all settings, if connected to a 2150. Disconnect in Flowlink.

12. Check the bottle carousel. Replace any bottles with water in them. Stop and restart the program on the Isco 6712 to ensure sampling will start at Bottle 1. Make sure that the 6712 program shows that the sampler is enabled to sample.

13. Disconnect the Isco connection cord from the sampler, and secure any caps.

Sampling: 1. The second staff member will record the sample date and time on the whirlpak, on the lab sheet, and in the laptop field form. Ensure the sample dates and times are recorded the same in all three. Make the time a multiple of 5 for ease of recording. This also helps denote a grab sample when looking through lab data.

2. Gain access to the sampling area. Open the manhole using the manhole hook or open the vault doors using a T-wrench if needed. Location specific grabs are: â&#x20AC;˘ â&#x20AC;˘ â&#x20AC;˘

At Villa Park Inlet, remove the grate over the drain and collect the sample from the water overflowing through the grate.

At Villa Park Outlet, reach down between the grate and collect the sample, trying not to let the bag touch the bottom of the culvert.

At Kittson, use the manual pump function on the 6712. Disconnect the pump tubing at the location where it enters the sampler bottle housing. Using the keypad on the Isco 6712 Sampler, pump water forward until water is flowing from the tube. Allow the water to flow through the tube for at least 30 19

seconds to rinse the line. Pump water directly into the E. coli whirlpak, then place in cooler. Pump water backwards for approximately one minute to purge the sample line. Reconnect the pump tubing to the bottle housing on the sampler when completed.

3. Attach the appropriate E. coli bag to the E. coli sampler. Ensure your fingers do not touch the inside of the whirlpak bag as this can contaminate the sample. Lower the E. coli bag into the water. Retrieve the sample. The water level inside of the bag should be above the white line on the whirlpak. If the water level is not above the white line, try again. This can take several attempts. Once enough sample water is retrieved, secure the whirlpak, check to make sure the bag is not leaking, and place the sample inside a small cooler. â&#x20AC;˘

At locations with high/fast flow, it is often difficult to fill the E. Coli bag to the desired level. At these locations, follow these steps. First, lower bag until it is submerged in top of water. Keeping bag at current position, let out slack from rope and hold on to it at the top in one hand. In one motion, release all the slack on the line at once to allow bag to fully submerge and be carried downstream.

Proceed to retrieve sample.

4. Secure the manhole or vault door.

Before Leaving the station: 1. Take pictures of any abnormalities in the field. This can include but is not limited to a noticed illicit discharge, abnormally dark water in sample bottles, or equipment damage.

2. Check to ensure the sampler is enabled and is ready to sample.

3. Check to ensure the sampler is aligned correctly on the bottles, and all tubing is attached appropriately.

4. Ensure all of the connector covers are secured on back of the sampler or flow logger.

5. Check that the E. coli sample dates and times are the same on the whirlpak, lab sheet, and in the laptop field form.

6. Check the station for any remaining equipment.

20

7. Secure the lock on the sampler box.

Day 2: Collecting Samples, Downloading and Changing the Programming: 1. Stop the program if it is still running. Remove the bottle carousel from the Isco sampler.

Cap all of the bottles containing sample water. Write the abbreviated station name on Bottle #1. This will identify the carousel with the correct station when compositing. If no, or insufficient samples are retrieved, see the “Base Grab Sampling (SOP)” and troubleshoot the problem. Replace with new carousal containing clean bottles. *In 2014, CRWD is working with Ben Janke from the University of Minnesota. When collecting a base composite, make sure that an additional sample is poured into a smaller labeled container when compositing samples at the office, and stored for him to come and collect.

2. One staff member will connect to the 2150 using the laptop computer and Isco connection cable. The second team member will begin with the sampling (see the Sampling section). Once connected, download the data using Flowlink.

3. Record the level, velocity, and calculated discharge from Flowlink into laptop field form.

4. Using the voltmeter, connect to the marine battery terminals. Record the marine battery charge and current battery ID in the field form. If the battery charge is below 70% or 12.3V, replace the battery once you are no longer “connected” in Flowlink. For those stations that have marine battery power for the 2150, also replace the battery if it reaches 12.3V. 5. Check the internal battery charge of the Isco 2150 Flow Logger, if present, by going to the “Measurements” screen in Flowlink. Record the internal battery voltage in laptop field form. If the voltage is below 10.2V, replace the 6V batteries inside the Isco 2150 Flow Logger once Flowlink is disconnected.

6. For stations with 2150 flow loggers, check the desiccant of the Isco 2150 Flow Logger and Isco 2105 interface module. If the desiccant is blue or orange, the desiccant does not need to be replaced. If the desiccant is pink or green, replace the desiccant.

21

7. Once the data is finished downloading, check the data. Record any missing data or abnormalities in the laptop field form. Also check the data to ensure flow levels correspond to baseflow conditions, note any abnormalities in laptop field form. If there are periods where it is obviously not baseflow (an abnormal level rise is observed), do not include any of those samples in the base composite.

8. With the station data open in Flowlink, record the sample start and end times on the lab sheet and the laptop field form. Record the number of samples taken, the volume that will be sent to the lab (If the volume collected is less than 4000mL, write the actual volume. If the volume collected is greater than 4000, write 4000mL), and the actual bottle numbers included in the sample.

9. Record the current pacing and trigger into the laptop field form (See “Isco Instrument Pacing SOP”).

10. Change the program back to the “Storm” equation for the sampler enable, and revert the pacing to the previous storm condition pacing. Apply all changes.

11. Stop and restart the program on the 6712 to ensure the sampler is ready to sample starting at Bottle #1. Ensure it is reading “Disabled”.

12. Disconnect in Flowlink and disconnect the data cords. Continue changing the battery or internal desiccant if needed.

Before Leaving the station: 1. Take pictures of any abnormalities in the field. This can include but is not limited to a noticed illicit discharge, abnormally dark water in sample bottles, or equipment damage.

2. Check to ensure the sampler is reading “Disabled” and is ready to begin sampling at Bottle #1.

3. Check to ensure the sampler is aligned correctly on the bottles, and all tubing is attached appropriately.

4. Ensure all of the caps are secured on back of the sampler or flow logger.

22

5. Check that the sample dates and times are the same on the lab sheet and in the laptop field form.

6. Check the station for any remaining equipment.

7. Secure the lock on the sampler box.

At the shop: 1. Follow the procedures outlined in the â&#x20AC;&#x153;Sample Compositingâ&#x20AC;? SOP. 2. Clean up and put away all equipment.

3. Charge any used batteries and/or spent desiccant.

4. Check over the field notes and sample times in the field form. Advise technicians of any problems that occurred in the field. Note any repairs needed on the white board in the hallway.

23

24

2D. Storm Composite Sampling Purpose: To collect a representative series of storm samples from all station s with Isco 6712 Samplers for lab analysis.

General: The storm composite procedures can be completed at all station s with Isco 6712 Samplers following a storm event. Following a storm, the station data is downloaded and checked, the sample carousel is collected, and regular station maintenance is performed.

Frequency: During the monitoring season, when rain events occur Locations: All stations Number of Staff: 2 Expected Time for Completion: One day for Baseline stations, and one day for BMP stations including sample prep and delivery • •

One day for both routes if only working with 1 team of 2 people A half day for both baseline and BMP stations if working with 2 teams of 2

Equipment: •

Clipboard with: o Sharpies o Pens

•

Lab sheet (Met Council)

•

Backpack with: o Isco Sampler connection cords o Ziplock bag containing charged desiccant (both in tubes and beads) o Ziplock bag for spent desiccant o Voltmeter o Extra pump tubes

•

Sampling Equipment: o Containers of large and small caps o Compact Carousels o Full Size Carousels 31

o T-wrench for vault doors (TBO, TBEB, TBWB) o Manhole hook (for Phalen) •

Record Maintenance Equipment: o Laptop o iPad o Waterproof camera

•

Miscellaneous items: o Marine batteries (for all stations – sampling can drain batteries) o 6V batteries (for 2150 stations) o Tool box o Lock key (in truck glove box) o Hand sanitizer (in truck glove box)

Procedure: Before Leaving the Shop: 1. If any samples are to be submitted to the Met Council, e-mail the Met Council lab in the morning before sampling. See monitoring coordinator for current list of e-mail addresses. Be sure to note: • •

The approximate number of full water quality samples The approximate time of arrival to the Met Council

Since no E. coli samples are taken for storm composites, you may deliver full water quality samples to the lab the following day (with the exception of Fridays. On Fridays, samples need to be turned in to the lab ASAP, as they lab staff cannot wait to process them the next day). Check the hold times for the lab parameters.

2. Both field team members will check over the equipment list before leaving the office.

In the Field: 1. One staff member will connect to the 2150 using the laptop computer and Isco connection cable. If a 750 module is being used, connect directly to the 6712.

32

2. The second team member will remove the bottle carousel from the sampler and align a clean carousel in the sampler. Cap the bottles, and write the abbreviated station name on the first bottle lid. This identifies the carousel with the station when compositing.

Once finished, assist with the remaining procedures.

3. The staff member using the laptop will download the data using Flowlink. Downloading takes a few minutes, continue with procedures until downloading is completed.

4. Record the level, velocity, and calculated discharge from Flowlink into the laptop field form.

5. Using the voltmeter, connect to the marine battery terminals. Record the marine battery charge and current battery ID in the laptop field form. If the battery charge is below 70% or 12.3V, replace the battery once you are no longer “connected” in Flowlink. For those stations that have marine battery power for the 2150, also replace the battery if it reaches 12.3V. 6. If the 2150 is powered by 6V batteries, check the internal battery charge of the Isco by going to the “Measurement” tab in Flowlink. Record the internal battery voltage in the laptop field form. If the voltage is below 10.2V, replace the 6V batteries inside the Isco 2150 Flow Logger once disconnected in Flowlink.

7. For stations with 2150 flow loggers, check the desiccant of the Isco 2150 Flow Logger and Isco 2105 Interface Module. If the desiccant is blue or orange, the desiccant does not need to be replaced. If the desiccant is pink or green, replace the desiccant.

Periodically check desiccant in 2191 battery compartments.

The desiccant of the Isco 750 module is housed on the outside of the module. If the desiccant is yellow, then the desiccant does not need to be replaced. If the desiccant is green, then replace the desiccant.

8. Once the data is finished downloading, check the data in Flowlink. Review the graphs in Flowlink for level, velocity, and sample events. Note any abnormalities or missing data in the laptop field form. If applicable, adjust the trigger and pacing so it improves sampling for the next storm event. When examining the hydrograph, check for any potential illicit discharges that may have occurred (e.g. East Kittsondale). If level rises when there is not a known precipitation event, this could be a potential illicit discharge, and the sample

33

should not be considered a normal storm event. Make a note of it in the field form and discuss with other technicians. Disconnect from Flowlink when completed.

9. With the station data open, review the sample times. Record the date and time of the first sample and last sample in the laptop. Also, record the number of samples, the sample total volume and the bottle numbers included in the composite (e.g., 1-24). If no samples were taken or the volume of sample water is not sufficient, dump the bottles, document the number of dumped bottles and the problem, and troubleshoot the sample problem. (see “Troubleshooting SOP”) *If there is a large gap (>4 hours) in sampling and two storm hydrographs are present in Flowlink, document each storm separately in the field form. Note the start and end times for each storm, the sample volume, and the bottle numbers for each storm. Write “#1” on the first bottle, and “#2” on the bottle where the second storm begins. If a bottle is a mixture of the two storms, dump that bottle and note the dumped bottle number in the field form. **If unsure of how to separate storms, or what should be composited, leave all samples in carousel and bring back to the shop to discuss with the team.

10. Disconnect Flowlink and cords; continue changing the battery or internal desiccant if needed.

11. Check to ensure the sampler is reading “Disabled” on the Isco sampler display and is ready to sample starting at Bottle #1. Stop and restart the program if needed.

Before Leaving the station: 1. Take pictures of any abnormalities in the field. This can include but is not limited to a noticed illicit discharge, abnormally dark water in sample bottles, or equipment damage.

2. Check to ensure the sampler is aligned correctly on the bottles, and all tubing is attached appropriately.

3. Ensure all of the caps are secured on back of the sampler or flow logger.

4. Check the station for any remaining equipment.

5. Secure the lock on the sampler box.

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Back at the shop: 1. Follow the procedures in the “Sample Composting” SOP to composite storm samples.

2. Clean up and put away all equipment.

3. Charge any used batteries and/or spent desiccant.

4. If a potential illicit discharge occurred, inform the monitoring coordinator and TBI project manager ASAP, and discuss whether to inform the City of St. Paul (see “Illicit Discharge Grab Sampling” SOP for more details). Divide the samples collected according to storm and illicit discharge and fill out the COC for an illicit discharge event.

5. Check over the field notes and sample times in the field form. Advise technicians of any problems that occurred in the field. Note any repairs needed on the white board in the hallway.

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36

3H. Isco 6712 Volume Calibration Purpose: To calibrate the sample volume on Isco 6712 samplers for composite sampling General: Each full water quality station with an installed Isco 6712 sampler has the capability to take composite samples. The volume of the samples must be calibrated at the time of installation and as needed during the field season. To calibrate the sample volume at stations with no base flow, one person must access the location of the sieve and place the end in a bottle of water. Using the keypad on the sampler, the sample volume is calibrated.

Frequency: At the time of installation, and as needed throughout the field season Number of Staff: 2 team members (Confined Space Entry certified if needed) Locations: Any station with an Isco 6712 Expected Time for Completion: 5 minutes to 45 minutes, depending if confined space entry is needed Equipment: • • • • • • •

2 – 4000mL bottles of tap water per each station without baseflow Graduated cylinder (located in truck) Ruler (usually level calibration is performed at the same time) Isco 6712 sampler Laptop iPad One extra marine battery

Confined Space Entry Equipment (if needed): • • • • •

• • •

Manhole hook or T-wrench Tripod Winch (check to ensure it is wound properly) Lifeline Gas Meters (at least 2 (with one being the new Quattro), with extra batteries and screwdriver) Walkie-Talkies (check to ensure they are fully charged) Flashlight, head lamp, and/or stick-light Helmets with headlamps 139

• • • • • • • •

Rubber boots, hip waders, and/or waders Buckets with ropes Harness Gloves Chain for tripod Safety vests and fencing (if needed) PFD (if needed) Any equipment needed to perform the duties at the station

Procedure: Before Leaving the Office 1. If confined space entry is needed, ensure at least two team members going to the location are Confined Space Entry certified. Advise team members in the office of the plan for the day, and the stations you will be entering. Review the Confined Space Entry SOP. 2. Check over all of the equipment. Do any batteries need replacing? Are the lights, gas meters, walkie-talkies charged? Is any of the equipment damaged?

3. Check the weather. DO NOT enter any confined space during storm conditions. Even a small amount of rain can produce dangerous conditions below ground. Keep an eye on the weather when out in the field. Even if it is not raining at your current location, it may be raining upstream. Weather can change quickly, so be prepared.

4. Pack the equipment into the truck. All team members should check over the equipment thoroughly. Forgotten equipment can result in dangerous situations or time delays in returning to the office.

Upon Reaching the station: 1. If confined space entry will be performed, review and follow the “Confined Space Entry SOP.” 2. If the station does not have a base flow, open the station location using the manhole hook or T-wrench. Remove the sampler or logger if needed. If the station does have baseflow, skip to Step 4.

140

3. The entrant will access the sieve, clip any zip-ties securing the sieve, and place the end of the sieve inside a 4000mL bottle of water.

4. The attendant will stop the program and prepare for the calibration: a) b) c) d) e) f) g) h) i) j)

k) l) m) n)

Press the red button Using the arrows on the keypad highlight “Stop Program” Press “Enter” (the yellow button with the arrow pointing left) Using the arrow on the keypad highlight “Other Functions” Press “Enter” Using the arrows on the keypad highlight “Manual Functions” Press “Enter” Using the arrows on the keypad highlight “Calibrate Volume” Press “Enter” Using the arrows on the keypad highlight either “Standard Portable” or “Compact Portable.” Standard Portable is for samplers with large bottles. Compact Portable is for samplers with small bottles.

Press “Enter” Using the keypad, enter the desired volume (in mL). Usually this is 200 mL. Press “Enter” Remove the pump tubing from at the location where it attaches to the bottle housing, and place the end of the hose inside the graduated cylinder. The sampler will first pump backwards to purge the sample line. Then forward to dispense the desired sample amount. Then backwards again to purge the sample line.

5. Read the sample volume. If the sample volume is +/-50mL of the desired volume, type “200” with the keypad, press “Enter,” then skip to Step 6. If the sample volume is outside this range, then incrementally adjust the sample volume, and repeat Steps 4 and 5 until the sample is within the desired range. ***Note: The samplers will often over adjust for changes to the sample volume. If you receive a sample of 300mL, type into the sampler around, “220.” If you receive a sample of 140mL, type into the sampler “180.” By incrementally adjusting the sample volume, you reduce the risk of overshooting the desired sample volume.

6. Once the desired sample volume is reached, the entrant can take a level reading and perform a level calibration if needed (see “Level Calibration SOP”). The entrant can exit the confined space.

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7. Restart the program by: a) b) c) d) e)

Pressing the red button on the keypad to return to the Main Menu Highlight “Run Program” Check that the sample bottle starts at 1.

Press “Enter” The display screen should state “Disabled”

8. Secure the pump tubing to the bottle housing.

9. Complete the respective station visit form. Include in the maintenance section that a volume calibration was performed. If you were unable to complete the volume calibration, see the “Troubleshooting SOP.” Before Leaving the station: 1. Take down and secure all equipment in the truck. Check the station for any stray equipment.

2. Check the iPad Confined Space Entry Form is completed, and any notes are completed in the iPad or laptop field form.

3. Make sure the caps are secured on the back of the sampler and hoses are intact.

4. Close the manhole and lock the lockbox At the Shop: 1. Clean up and put away all equipment.

2. Check over the field notes. Advise technicians of any problems that occurred in the field.

Note any repairs needed on the white board in the hallway.

142

3G. ISCO 2150 Level Calibration Purpose: To calibrate measured level on the Isco 2150 Flow Loggers.

General: Isco 2150 flow loggers are the most sensitive of the three types of loggers we use. We generally install them in locations where the water levels are very low such as the trenches and in our winter monitoring stations. These modules will provide data about the velocity and the level of a station. Each month during the field season the level is calibrated at all stations. The level is also calibrated when the reviewed data appears to be incorrect. To calibrate the level, two people are required. One person remains at the surface and completes the programming and notations. The second team member will access the location of the Area/Velocity sensor and take a measurement of the water level in front of the sensor.

Frequency: At the time of installation, monthly during the field season, if battery has been dead for an extended period, and as needed for troubleshooting.

Number of Staff: 2 Locations: Most stations and winter monitoring stations.

Expected Time for Completion: 10 - 45 minutes, depending on if confined space entry is needed to access the station Equipment: • • • • • • •

Isco 2150 Flow Logger Isco 2150 download cable Lock box key (in glove compartment of truck) Ruler Laptop iPad (for maintenance notes) Camera

Confined Space Entry (determine equipment as needed to gain access to the probe): • • • •

Manhole hook Tripod Winch (ensure that it is wound properly) Lifeline 135

• • • • • • • • • • •

Gas Meters (both, with extra batteries and screwdriver) Walkie-Talkies Flashlight, head lamp, and/or stick-light Helmet Rubber boots, hip waders, and/or waders iPad (confined space entry form) Buckets with grab sampler ropes Harness Gloves Chain for tripod Safety vests and fencing (if needed)

Procedure: 1. Open the lock box and manhole as needed. Remove the logger from the manhole if needed. If confined space entry is needed to access the sensor, one team member will need to enter the station to access the sensor. Follow the “Confined Space Entry SOP.” 2. Once the sensor is accessed, take 2-3 measurements of the water level. The measurement is of the actual water level in the pipe, so the measurement should be taken from the bottom of the pipe on the cement to the surface of the water. Turn the ruler parallel to the flow, so interference from the ruler when measuring is minimized.

Advise the team member at the surface of the water level. Check for debris blocking the sensor. Advise the team member on the surface of any rocks, leaves, or other debris removed from the sensor. The entrant can exit the space if no further action is needed at the station.

3. The team member at the surface will connect to the 2150 using Flowlink, and observe the current level. If the manual level reading differs from the level being read by the sensor by >0.1in or more, adjust the level of the 2150 reading using the following steps: a) Connect to the 2150 Flow Logger (See “2150 Flow Logger Download” SOP) b) Click the “Jump to measurement tab,” select the module you wish to calibrate and navigate to “level” c) Enter the new level into the “Adjusted level” field d) Click “Apply” at the bottom of the screen or press F9 e) Make sure that the “Level measurement” line on the same screen is reading the newly adjusted level.

136

f) It may take a few seconds for the new level to show up. If not, try entering the adjusted level again.

4. Document any changes in the level by clicking “Yes” under “Program changed?” in the field form. Note that a level calibration was performed under the “Maintenance” field.

Complete the Confined Space Entry Form with the exit time.

Before Leaving the station: 1. Take pictures of any abnormalities in the field. This can include but is not limited to a noticed illicit discharge or equipment damage.

2. Check to ensure the logger is secured properly if suspended in a manhole.

3. Check that all protective caps have been put back into place.

4. Ensure the Confined Space Entry form is completed with the exit time.

5. Ensure the field notations are completed in the field forms.

6. Check for any equipment left in the field and that all locks and manholes are secured.

At the Shop: 1. Clean up and put away all equipment.

2. Check over the field notes. Advise technicians of any problems that occurred in the field.

Note any repairs needed on the white board in the hallway.

137

138

7F. Importing Flowlink Data into WISKI Purpose: To transfer data collected with Isco Flowlink into the WISKI software database.

General: All of CRWD’s continuous monitoring data resides in the WISKI software database. All data collected from Isco products in the field is stored in Flowlink databases on the field laptops. This data must be transferred to WISKI for QA/QC and reporting/analysis of the data.

Frequency: Once per week (Friday) Number of Staff: 1 Expected Time for Completion: 10 Minutes Equipment: • •

Field computer with Flowlink software Access to CRWD server, preferably through a wired connection.

Procedure: Flowlink A scheduled task called “Manual Site Backup” is saved under the “Schedules” folder in the left pane of Flowlink.

•

To export the data for all sites, right click on the “Manual Site Backup” task and select “Run Now”

Free File Sync Once the data has been exported from flowlink, it still needs to be uploaded to the server. This task has been partially automated using the program “Free File Sync” Before Running Free File Sync, make sure that all sites exported properly by checking the export folder in C:\Flowlink_Exports\ and looking at the timestamp (date created). All files should have timestamps with the current date. The export process can take some time so do not panic (yet) if a site did not export. Give it some time and check again.

•

Run Free File Sync and transfer the newly exported Flowlink data to the server by double clicking the desktop shortcut named “Sync Flowlink to Server”

Flowlink Notes: Each site has a graph template saved that includes the parameters Level, Velocity, and Temperature for the entire year (2015).

•

The graph templates in Flowlink have been set up to display 1 year of data. This is initially set to 2015. To alter this date range for another year e.g. 2016, open the graph properties, change the date range and re-save the graph template (File>Save)

The “Manual site backup” task has a number of sub-tasks that export the data defined by the graph template to the local directory: C:\Flowlink_Exports\ • •

To edit the task, right click and select “open” To add a new task right click on “Manual Site Backup” and choose “New Task” o Select “Export to a file” o Select the graph to export o Select the location to save the export (C:\Flowlink_Exports\) o Click OK to save

Free File Sync Notes: Free File Sync will sync the local folder “C:\Flowlink_Exports\” with the server folder \\crwdwiski\WISKIImports\ISCO_Import The sync is one-way. Changes made on the local drive are made to the server folder but not vise-versa. Files synced to this folder will not remain there for long. A program (KIDAT) running on the WISKI server will import the files into the WISKI database. Imported CSV files are moved to \\crwdwiski\WISKIImports\ISCO_Import\Done and appended with a timestamp.

WISKI importing important Notes: The KIDAT software, which is responsible for importing the exported CSV files into WISKI matches “exchange numbers” which are set up within individual WISKI time series’ with the text on specific lines of the CSV file. It is therefore important that these match, or the import will fail.

Flowlink CSV exports

•

•

•

KIDAT combines the site name in a given column (line 1 of CSV) with the label (line 3 of CSV) replacing all spaces with underscores. The exchange number it looks for is then: [site_name]_[label].

The label can be manipulated in the graph template. This is necessary for sites with multiple 2150 modules. Flowlink will export the exact same site name and label for each 2150 in a multiple 2150 setup. For example, the hidden falls 30 inch pipe and outlet pipe are both exported with a site name of “HiddenFalls” and a label of “Level” (for the level time series). KIDAT will then use “HiddenFalls_Level” as the exchange number for both.

This will result in both 30 inch pipe and outlet data imported into the same time series.

This is not correct. However, there will be no warning message about this because KIDAT and WISKI believe this to be correct behavior. The label for the 30 inch pipe can be changed in the graph template to “30Level” resulting in a unique exchange number of “HiddenFalls_30Level” KIDAT assumes that the units in the CSV are the default units for the time series in WISKI. If an import is being done for the first time, verify that this is true by double checking the default units setting in Flowlink.

Global Water • •

KIDAT combines the site name in the top left column with line 2 of a given column of data to create the exchange number in the format [site_name]_[line 2].

All spaces in the text of cells are replaced with “_” (underscore).

In-Situ • •

KIDAT combines the logger serial number in column 3 line 6 with the parameter on line 12 of a given data column to create the exchange number.

All spaces in the text of cells are replaced with “_” (underscore).

10E. Importing SW Lab Data into WISKI Purpose: To update the lab data in WISKI through uploading the most recent lab data spreadsheet sent from MCES. General: Updating lab data in WISKI will occur every time we see updated lab data within the MCES database. This entails exporting the data from the MCES online database to a .csv file, modifying the .csv file, and uploading it to WISKI using the Import Samplings agent.

Frequency: Every time new lab data gets updated to the MCES online database Number of Staff: 1 Expected Time for Completion: 15 minutes Equipment: • • •

WISKI MCES database open online (https://bi.metc.state.mn.us) Lab COCs

Procedure: Exporting data from MCES database: 1) Open the MCES database at https://bi.metc.state.mn.us with the following information: a. Username: Client_130 b. Password: CRWD@1410! c. Authentication Portal: Enterprise 2) Double-click on “Client Report Capitol Region Watershed District”. When prompted, enter the start and end dates for the range of data you would like (you can change the range at any time once the report is open).

3) Select “Cross Tab Report” (either in the navigation map on the left-hand side, or in the tab on the bottom of the main window).

4) Using the export icon in the upper left (see below), select “Export Current Report as CSV Archive.” Keep the current selections for export and click ok.

5) When the zipped file appears in the download bar, double-click to open. Double-click on the Cross Tab Report.csv file in the .zip folder.

6) Save as “MCES Lab Data Export_mmddyyyy” in “W:\07 Programs\Monitoring & Data Acquisition\2016 Monitoring\2016 Lab\Exported from MCES Database” using the format of files already in the folder.

7) Delete the top three rows (sometimes this is only two rows, depending on whether or not staff changed the output format – delete the top rows that are blank with only the project ID number in the uppermost left cell).

8) Scroll to the column with the header “P-AV”. Move this box to the box below (“Total Phosphorus (mg/L)”), or all of these values will overwrite the other Total Phosphorus (NUT-AV) values from the preceding column.

9) Verify that all of the info columns (measuring program, date, etc) do not have any blanks. Also verify that COMP and GRAB are not plural, as WISKI will not recognize these as correct key list entries.

10) Check through the Sample Status column, represented by the letters below. If there are outstanding values, just know that these will be updated in future. a. A = value is authorized and approved as final b. V = certain values in sample are not finished/outstanding c. C = all data values are complete and waiting to be authorized d. X = the whole sample was cancelled (the lab should have notified us) 11) Save this file as a new spreadsheet in the folder “W:\07 Programs\Monitoring & Data Acquisition\2016 Monitoring\2016 Lab\FINAL imported into WISKI” using the format of files already in the folder and close the file after finalizing.

Importing data into WISKI database: 1) Select Import Samplings from the dropdown menu.

2) Select ImportNewLabData. Verify that the mapping selected is ParamMapLabNew, column info is in line 2, and there are 3 header lines.

3) In the “Import” tab, using the “…” icon on the far right, open the most recent lab data .csv file that you just edited in the file “W:\07 Programs\Monitoring & Data Acquisition\2016 Monitoring\2016 Lab\FINAL imported into WISKI”.

4) Click “Read File”. Once the file has been read, the window will fill with the status of each line of the file. Click on the “Status” rectangle at the top of the window to sort the status lines. There are various statuses that can be observed: a. “OK (New)” means there are no problems with the line of data. b. “OK (New) Value without unit” means that a unit isn’t specified within the .csv being uploaded. That’s also fine. c. “OK (Update) Value without unit” means that the sample date is already in the system, and is being updated (usually with a parameter value that is being updated that was not previously there). That’s also fine. d. Errors come with a description of the error (i.e.: “Could not find sampling parameter by header” means that there is a column of lab data with a header that is not defined by the Parameter Mapping in the Configuration Tab).

5) If any errors are found, address them at this time (i.e. go back into.csv file, fix errors, and reimport/read the file). Ask a member of the MRM team to verify any errors or concerns.

6) Click on “Check Sample Results”. This tab looks through the current data in WISKI and compares the data you are trying to import. If there are any values that differ (i.e.: the lab updated or changed any previously entered data), this tab will show you the New Value and the Old Value. This allows you to QA/QC the lab data to see any discrepancies in the data between the previous and most recent versions of the data. a. Note the color legend at the bottom of the window that denotes if the value is new, updated, etc. You can click on the top of the color column to sort by color. b. If the update or change is valid and reasonable (e.g.: the old value was <0.002 and the new value is 0.0016, which is clearly an update), select “Accept” from the dropdown menu. c. If the update or change does not seem right where the value is clearly something different, select “Reject” from the dropdown menu. d. Note the rejected values and go find them in the previous and most recent spreadsheet. Note the date/time and sample values and contact the lab as to why the discrepancy is occurring.

7) Once all errors and any updated sample results have been addressed, select “Import Data” at this time.

8) To view the recently imported lab data, change the perspective to WQM under the File dropdown menu. Then under Station List in the WISKI Explorer window, select the site you wish to view, then select the Measuring Program for which you would like to view lab data.

10F. TP/TSS Outlier Identification in WISKI Purpose: To identify outliers in lab data in WISKI through evaluation of the TP and TSS lab values and their comparison to other nutrient parameters.

General: Identifying outliers is completed in WISKI by identifying values outside of thresholds for both TP and TSS, and comparison these identified values to TKN and Ortho-P. Frequency: 3 times per year (Identifying outliers can occur a few different times a year, ideally after winter/snowmelt monitoring is complete, after summer/field season monitoring is complete, and again at the end of the year prior to analyzing and compiling data for reporting.)

Number of Staff: 2-3 Expected Time for Completion: 30 minutes Equipment: â&#x20AC;˘

WISKI

â&#x20AC;˘

Google drive field forms open (to reference field notes, if needed)

Procedure: 1) Open the EventSamples time series for TP for the site and time range you are interested in. On the same graph, open the EventSamples time series for TSS for the same site.

2) In the icon bar across the top of the graph, click on the regression icon and select Regression (simple). Select the Power Law regression line and click ok. In the graph, apply timestamp labels to the graph.

3) Using the bounds of 1.5 mg/L for TP and 2000 mg/L for TSS (thresholds determined by MRM staff during historical lab data outlier analysis through discussion), write down all dates/times for all values of TP at or above 1.5 mg/L and all dates/times for all values of TSS at or above 2000 mg/L. 4) Compare each point to the respective TP/TSS value. Open the EventSamples time series for TKN and Ortho-P, and determine the values for these parameters. Compare against other nutrient parameters to determine if the identified outlier is valid or invalid. For example, the following scenarios could occur: a. The value is very high, but all other nutrient values are very low, and the event sample is a composite with a full 4000ml submitted. This is likely a result of a lab error, and is an erroneous data point. b. The value is very high, other nutrient values are low, but the event sample was a grab, or something during collection occurred that reduced or concentrated the

sample volume. This is likely a result of a collection error, and the data point from the lab is valid, but the value is not representative of the sampled event. c. The value is high, other nutrient parameter values are high and thus the value is determined to be valid.

5) It is also helpful to consult the field notes to see if there is something noted about the sample or sample event that could explain the value observed. Staff can also check the value against other lab parameters from the sample, including E. Coli.

6) Use the following table to determine the quality code to give the identified outlier: Code Number Meaning Excellent 0 Good 40 Default for data that has been imported Fair 80 Sample evaluated, determined valid after comparison to other nutrient parameters Suspect 120 Poor 160 Sample evaluated, determined to be valid, but not represented due to collection error Sample evaluated, determine to be erroneous, potential lab error Unknown 200 Missing 255

Remark

Determined to be valid Not representative - collection error Erroneous - lab error

7) To change the quality code for any sample value, first change the perspective to WQM. Find and double-click on the sample in the dropdown menu for the site. Click on the Sample results tab and scroll down until you find the respective value.

8) Double-click on the value and a pop-up box will open for the specific data point. From the Quality dropdown menu, select the appropriate quality. In changing the quality, WISKI will force you to write a remark. Type in the respective remark from the above table. Click ok.

9) Verify with team members the actions taken.

Appendix C Sample Chain of Custody

97

98

CRWD SAMPLES: MCES Lab: 651-602-8293 MCES Fax: 651-602-8255

Date Submitted: Submitted By:

Storm Water Monitoring Project Project ID: 5533-05-01

Location Id: St. Anthony Park Samp Pt: CRWD1 Start Date / Time: End Date / Time: Location Id: Hidden Falls Outlet Samp Pt: CRWD45 Start Date / Time: End Date / Time: Location Id: East Kittson Samp Pt: CRWD2 Start Date / Time: End Date / Time: Location Id: Phalen Creek Samp Pt: CRWD5 Start Date / Time: End Date / Time: Location Id: Trout Brook Outlet Samp Pt: CRWD3 Start Date / Time: End Date / Time: Location Id: Trout Brook - East Branch Samp Pt: CRWD8 Start Date / Time: End Date / Time: Location Id: Trout Brook - West Branch Samp Pt: CRWD4 Start Date / Time: End Date / Time: Location Id: Villa Park Outlet Samp Pt: CRWD10 Start Date / Time: End Date / Time: Location Id: Villa Park Inlet Samp Pt: CRWD11 Start Date / Time: End Date / Time:

Measuring Program & Sample Type

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM:

Capitol Region Watershed District 1410 Energy Park Drive, Suite 4, St. Paul, MN 55108 Phone: (651) 644-8888 Fax: (651) 644-8894

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

CRWD SAMPLES: MCES Lab: 651-602-8293 MCES Fax: 651-602-8255

Date Submitted: Submitted By:

Storm Water Monitoring Project Project ID: 5533-05-01

Location Id: AHUG Inlet Samp Pt: CRWD14 Start Date / Time: End Date / Time: Location Id: Como 7 Samp Pt: CRWD6 Start Date / Time: End Date / Time: Location Id: Como 3 Samp Pt: CRWD21 Start Date / Time: End Date / Time: Location Id: BDale Outlet Samp Pt: CRWD41 Start Date / Time: End Date / Time: Location Id: Franklin Samp Pt: CRWD46 Start Date / Time: End Date / Time: Location Id: Dickerman Samp Pt: CRWD47 Start Date / Time: End Date / Time: Location Id: MLK Samp Pt: CRWD48 Start Date / Time: End Date / Time: Location Id: TBNS-Rose Samp Pt: CRWD43 Start Date / Time: End Date / Time: Location Id: TBNS-Stream Samp Pt: CRWD44 Start Date / Time: End Date / Time:

Measuring Program & Sample Type

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [

] ] ] ]

CBOD ORTHO_P SO4-N pH

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [ [

] ] ] ] ]

CBOD ORTHO_P SO4-N pH Fe-MSV

[ [ [ [ [

] ] ] ] ]

CL-AV HARD MET-MSV N_N-AV NH3N-AV

[ [ [ [ [

] ] ] ] ]

P-AV TDS-180 NUT-AV TSSVSS-GF ECOLI-MPNT

[ [ [ [ [

] ] ] ] ]

CBOD ORTHO_P SO4-N pH Fe-MSV

SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. SAMPLE MEAS. TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM: TYPE: PROGRM:

Capitol Region Watershed District 1410 Energy Park Drive, Suite 4, St. Paul, MN 55108 Phone: (651) 644-8888 Fax: (651) 644-8894

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

[ [ [ [ [

] ] ] ] ]

Base Storm Snowmelt Grab Composite

Appendix D Laboratory Quality Assurance Manual and Standard Operating Procedures

101

102

Metropolitan Council Working for the Region, Planning for the Future

Quality Assurance Manual Environmental Services Division Environmental Quality Assurance Department Laboratory Services 2400 Childs Road Saint Paul, MN

March 12, 2012

QA_MANUAL_2 Page 1 of 42

QA_MANUAL_2 Page 2 of 42

Metropolitan Council Working for the Region, Planning for the Future

QA MANUAL: TABLE OF CONTENTS Title Page.……………….…………………………………….…………………………………1 Signatures of Approval…………………………………………….…………………………… 2 Table of Contents………………...…………………………………….………………………3 – 5 List of Appendices…………..……………………………………………….…………………. 5 1. Introduction 1.1. MCES Laboratory Function 1.2. MCES Laboratory Vision and Mission 1.3. Laboratory Facility 1.4. Laboratory Certified Parameters 2. Laboratory Organization and Responsibility (4740.2099 A & B) 2.1. Laboratory Organization 2.2. Job Classification Specifications 2.3. Laboratory Manager of MCES Analytical Lab Services 2.4. Section Managers/Technical Managers 2.4.1. Section Manager Responsibilities 2.4.2. Inorganic Chemistry Section Manager 2.4.3. Biology/Residue Section Manager 2.4.4. Organic Analyses/Quality Assurance Section Manager 2.4.4.1. Organic Analyses Section Manager 2.4.4.2. Quality Assurance Officer 2.4.5. Analytical Support and Customer Service Section Manager 2.5. Analytical Section Staffing 2.5.1. Employment Requirements 2.5.2. Principal Environmental Scientist 2.5.3. Senior Environmental Scientist 2.5.4. Environmental Scientist 2.5.5. Technical Specialist 2.5.6. Technician III 2.5.7. Technician II 2.5.8. Technician I 2.5.9. Laboratory Assistant 3. Laboratory Personnel Training (4740.2099 D) 3.1. Demonstration of Capability 3.2. Certification of Analysts (2099 C) 3.3. Continual Analyst Certification (2099 E3a) 3.3.1. Failure of Continued Analyst Certification 3.3.2. Continual Analyst Certification Procedure 3.4. Identity Certification (2099 F) 4. Standard Operating Procedures (4740.2065) 4.1. Requirements (2065, Subpart 1 and 2) 4.2. Format of Analytical Procedures (2065, Subpart 3) 5. Laboratory Quality Assurance Practices and Objectives (4740.2080, 4740.2100 and 4740.2110) 5.1. Scope and Objectives(2080/2100 and 2110, Subpart 1) 5.2. Quality Control Criteria for Chemistry (4740.2100) 5.2.1. Method Blank (2100, Subpart 2) 5.2.2. Laboratory Control Sample (2100, Subpart 3) QA_MANUAL_2 Page 3 of 42

6.

7.

8.

9.

10.

11.

5.2.3. Quality Control Sample (2093, Subpart 3, C) 5.2.4. Matrix Spike/Matrix Spike Duplicate (2100, Subpart 4) 5.2.5. Surrogate Spikes (2100, Subpart 5) 5.2.6. Internal Standards (2100, Subpart 6) 5.2.7. Detection Limits (2100, Subpart 7) 5.2.8. Reporting Limits (2100, Subpart 8) 5.2.9. Selectivity (2100, Subpart 9) 5.2.10. Manual Integrations (2100, Subpart 10) 5.2.11. Uncertainty Measurements 5.3. Quality Control Criteria for Bacteriology (4740.2110) 5.3.1. Sterility Checks and Blanks (2110, Subpart 2) 5.3.2. Positive Controls (2110, Subpart 3) 5.3.3. Negative Controls (2110, Subpart 4) 5.3.4. Test Variability (2110, Subpart 5) 5.3.5. Method Evaluation (2110, Subpart 6) 5.3.6. Test Performance (2110, Subpart 7) 5.3.7. Quality of Standards, Reagents and Media (2110,Subpart 8) 5.3.8. Selectivity (2110, Subpart 9) 5.3.9. Temperature Measuring Devices (2110, Subpart 10) 5.3.10. Autoclaves (2110, Subpart 11) 5.3.11. Incubators, Water Baths and Ovens (2110, Subpart 13) 5.3.12. Labware, Glassware and Plasticware (Subpart 14) Proficiency Testing (4740.2070 and TNI Standard V1M1) 6.1. Proficiency Testing Scope 6.2. Laboratory Testing of PT Samples and Reporting Laboratory Sample Handling, Receipt and Acceptance (4740.2087) 7.1. Sample Handling, Sample Receipt Protocols and Preservation (2087, Subpart 1) 7.1.1. LIMS 7.1.2. NPDES Samples 7.1.3. Non-NPDES Samples 7.2. Sample Acceptance Policy (2087, Subpart 3) Standards, Reagents and Bacteriological Media (4740.2089) 8.1. Logbook Documentation for Chemicals and Reagents (2089, B) 8.1.1. Logbook Front Cover 8.1.2. Chemical Entries 8.1.3. Reagent Entries 8.2. Chemical and Reagent Bottle Labels (2089, C) 8.2.1. Chemical Bottle Labels 8.2.2. Reagent Bottle Labels Support Equipment Calibrations (4740.2091) 9.1. Scope of Support Equipment Calibration (4091,Subpart 1) 9.2. Support Equipment and Software Logbooks(4091, Subpart 2C) 9.2.1. Logbook Purpose 9.2.2. Logbook Front Cover Content 9.2.3. Logbook Content 9.2.3.1. Logbook Entries 9.2.3.2. Static Information 9.2.3.3. Active Information 9.3. Frequency of Calibration (2091, Subpart 3) 9.4. Support Equipment Maintenance Plans (2091, Subpart 3) 9.5. Documentation and Archiving of Logbooks (2091, Subpart 4D) Instrument Operational Validation (4740.2093) 10.1. Scope of Instrument Calibration Requirements (2091, Subpart 1) 10.2. Analytical Instrumentation and Software Logbooks (2093, Subpart 2) Analytical Response Calibration (4740.2093) 11.1. Analytical Response Calibration (2093, Subpart 3) QA_MANUAL_2 Page 4 of 42

11.2. Calibration Verification (2093, Subpart 4) 12. Laboratory Sample Reporting (4740.2095) 12.1. Data Reporting 12.2. Reporting Convention 12.3. Comments in the LIMS 12.4. Corrective Action Form (CAF) 12.5. Data Review and Authorization (TNI Standard V1M2: 4.2.8.4p) 12.5.1. Data Review Process 12.5.2. Multi-Level Product (MLP) 12.5.3. Logically Inconsistent Data (LID) 12.5.4. Peer Review and Authorization (TNI Standard V1M2: 4.2.8.4p) 13. Laboratory Records Retention and Retrieval (4740.2097) 13.1. Record Keeping and Retention Time (2097, Subpart A and B) 13.2. LIMS and Electronic Files (2097, Subpart D-J) 13.3. Paper Records (2097, Subpart D â&#x20AC;&#x201C; J) 14. Data Integrity Procedures (TNI Standard V1M2: 4.2.8.1) 15. Complaint Procedures (TNI Standard V1M2: 4.2.8.3n) 16. Management Reviews 17. Definitions (4740.2010)

LIST OF APPENDICES A. B. C. D. E. F. G.

Demonstration of Capability (DOC) for Chemistry Demonstration of Capability (DOC) for Microbiology List of Certified Standard Operating Procedures MCES Organizational Chart Test Codes by Analytical Section Analyst Certification Database Sample Acceptance Policy

QA_MANUAL_2 Page 5 of 42

1. Introduction to Metropolitan Council Environmental Services (MCES) 1.1. MCES Laboratory Function 1.1.1. The purpose of the Laboratory Services Business Unit is to provide clients with quality, timely, and cost-effective analytical services. The primary source of work for the MCES Laboratory comes from Council entities. The MCES Laboratory is a public agency and therefore does not have trade secret data. From time to time, the MCES Laboratory will provide analytical services to other entities, primarily, other government or non-profit environmental organizations. It is the policy of the MCES Laboratory to provide such external services without written or implied contracts. Such services are only provided in an identical manner to those services provided to internal clients. The services provided to these clients include, but are not limited to the following: 1.1.1.1. project development, 1.1.1.2. sample analysis, 1.1.1.3. sample collection and container preparation, 1.1.1.4. instrument quality assurance, 1.1.1.5. test method development, and 1.1.1.6. information management 1.2. MCES Laboratory Vision and Mission (4740.2085) 1.2.1. The Metropolitan Council and their employees are committed to quality and to protecting and improving our natural resources. As a part of our mission and commitments, MCES Laboratory has developed this quality assurance manual.

1.2.2. This quality assurance plan has been developed to completely comply with the Minnesota State Rules 4740.2010 through 4740.2040 and TNI Standard to provide the framework by which the laboratory will operate. The goal of the MCES Laboratory is to perform the level of quality control needed to assure a quality product. The level of the quality control is determined based on the needs of the client, the use of the data, and the cost of the associated quality control.

Procedures are in place to prevent deviations to and maintain the laboratory’s quality system by performing SOP reviews, internal audits and external audits.

1.2.3. The Metropolitan Council’s Laboratory Services management ensures the ongoing capability and quality of our staff by assuring compliance with the current Quality Assurance (QA) manual.

The manual is available via the LIMS and Intranet website and is updated periodically. The QA manual is reviewed annually and updated as often as needed when changes become apparent.

Laboratory staff are made aware of any changes through a labwide meeting or email notification.

Staff are asked to review the QA manual and comment where necessary.

1.2.4. Any quality control practices identified in this document that are not in the Minnesota State Rules, certification requirements, or the approved reference method are considered to be good laboratory practices and can be modified based on Data Quality Objectives (DQO’s) and/or the Quality Assurance Officer’s approval.

1.3. Laboratory Facility (2085, Section C. 2) 1.3.1. The Metropolitan Council Laboratory is located at 2400 Childs Road, Saint Paul Minnesota. The Environmental Planning and Evaluation (EPE) building was constructed during 1999-2000. The EPE laboratory is 30,000 square feet and has been in operation since April 2000.

1.4. Laboratory Certified Parameters 1.4.1. The Minnesota Department of Health (MDH) laboratory certification program issues a Scope of Certification annually which contains a listing of certified fields of testing for Metropolitan Council Analytical Services (Lab ID # 027-123-172). The current Scope of Certification is available on the laboratory’s intranet website. A list of all Certified parameters and their corresponding MCES SOPs can be found in Appendix C. 2. Laboratory Organization and Responsibility (2099 Section A and B.) 2.1. The Metropolitan Council’s Analytical Services is organized into an administrative group and four analytical sections. The administrative section is responsible for the management of the laboratory through standardized policies and practices and office support. The analytical sections consist of the QA_MANUAL_2 Page 6 of 42

Biology/Residue, Inorganic Chemistry, Organic/Quality Control and Analytical Support/Customer Service. The four analytical sections are responsible for sample handling, sample preparation, sample analysis, data review, data entry, and data validation. The laboratory organizational chart is shown in Appendix D. 2.2. Within analytical laboratory services there are a variety of technical and scientific positions.

Metropolitan Council maintains current job classification specifications for all personnel who manage, perform, or verify work affecting the quality of the environmental tests. Job classification specifications outlining the basic job duties and requirements for each position can be found in the MCES Human Resources Department’s files, located at the Agency’s central offices in St. Paul, Minnesota.

2.3. Laboratory Manager of MCES Analytical Laboratory Services 2.3.1. The manager is in charge of the MCES environmental analytical service laboratory and reports to the Assistant General Manager of the Environmental Quality Assurance Department. The Laboratory Manager is responsible for assuring the provision of environmental services of a known quality and ensuring the safety of the laboratory staff. The Management Association (MANA) Union represents the Laboratory Services manager position.

2.4. Section Managers/Technical Managers 2.4.1. Laboratory section managers are responsible for the training of staff, coordination of analyses, review, and validation of standard operating procedures, data review, data authorization, and method development within their section. They are also responsible for the direct supervision of the staff in their section. They serve as the Technical Manager of their respective section. They must meet the qualifications of TNI Standard V1M2: 5.2.6.1. In the event of an extended absence of a Technical Manager, QA will name an interim Technical Manager and document the duration served in the role. Section managers report to the laboratory manager. The Management Association (MANA) Union represents the laboratory section manager positions.

2.4.2. Inorganic Chemistry Section Manager 2.4.2.1. The inorganic chemistry section manager is responsible for anion cation, mercury, and metals analyses. The list of analytical parameters for this section is in Appendix E. 2.4.3. Biology/Residue Section Manager 2.4.3.1. The Biology/Residue section manager is responsible for the biological and gravimetric analyses and also coordinates, trains, and schedules weekend staff. The list of analytical parameters for this section is in Appendix E. 2.4.4. Organic Analyses/Quality Assurance Section Manager 2.4.4.1. Due staffing limitations, the Quality Manager (“Quality Assurance Officer”) has also been assigned responsibilities of Technical Director of the Laboratory’s Organic Analysis Section. Sufficient independent oversight systems exist to assure that any potential conflict of interest arising from this organizational structure are effectively mitigated.

2.4.4.2. Organic Analyses Section Manager 2.4.4.2.1. The organic analyses/quality assurance section manager is responsible for priority pollutant analyses. The list of analytical parameters for this section is in Appendix E. 2.4.4.3. Quality Assurance Officer 2.4.4.3.1. The quality assurance manager is the designated Quality Assurance Officer for the Laboratory and is responsible for providing quality assurance and quality control procedures and policies. Quality Assurance Officer and his/her designees are responsible for monitoring and maintaining ongoing compliance with QAQC procedures and policies. These procedures and policies include: 2.4.4.3.1.1. Standard Operating Procedures content, format, and maintenance, 2.4.4.3.1.2. Laboratory certification activities including Proficiency Testing (PT) sample distribution and result reporting, communication with MDH, and coordination/supervision of auditing activities including pre-audit preparation and internal audits, 2.4.4.3.1.3. Discharge Monitoring Report (DMR-QA) certification program activities and communications, QA_MANUAL_2 Page 7 of 42

2.4.4.3.1.4.

2.4.4.3.1.5.

2.4.4.3.1.6.

2.4.4.3.1.7.

Method development and implementation protocols, Analyst certification and training requirements, Quality Control/Batch protocols, Corrective Action activities and monitoring and reacting to out of specification trends, 2.4.4.3.1.8. Standardizing data reporting protocols 2.4.4.3.1.9. Standardized procedures for various activities such as record retention, container labeling, and overall QA protocols.

2.4.4.4. Analytical Support and Customer Service Section Manager 2.4.4.4.1. The analytical support and customer section manager is responsible for the training of technical staff working with LIMS, LIMS operation, development and maintenance.

The section manager also initiates projects with laboratory clients to ensure the laboratory has the capability of processing all analysis requirements requested by the client and provides all reported data to the clients. It is also the responsibility of the Analytical Support and Customer Service manager to maintain and develop laboratory safety programs, oversee building maintenance, sample cooler temperature tracking, glassware cleaning and container preparation. The list of analytical parameters for this section is in Appendix E. 2.5. Analytical Section Staffing 2.5.1. The laboratory sections are composed of laboratory technicians, scientists and laboratory assistants. American Federation of State, County and Municipal Employees (AFSCME) Union represents these positions. The following is a list of the positions within Laboratory Services and the minimum qualifications necessary for each position. Each employee of Metropolitan Council Environmental Laboratory Services must possess a valid drivers license and successfully complete a pre-employment physical, drug screen, and background check. Staffing positions are assigned according to the analytical complexity, needed for independent judgment, and leadership requirements in each positionâ&#x20AC;&#x2122;s job assignments.

2.5.2. Principal Environmental Scientist (AFSCME 32) 2.5.2.1. The Principal Environmental Scientist must have an undergraduate degree in chemistry or biology, as appropriate.

2.5.3. Senior Environmental Scientist (AFSCME 31) 2.5.3.1. The Senior Environmental Scientist must have an undergraduate degree in chemistry or biology, as appropriate.

2.5.4. Environmental Scientist (AFSCME 30) 2.5.4.1. The Environmental Scientist must have an undergraduate degree in chemistry or biology, as appropriate.

2.5.5. Technical Specialist (AFSCME 30) 2.5.5.1. The Laboratory Technical Specialist must have completed an approved technical/vocational laboratory technician training program or one year (2 semesters or 3 trimesters) of college chemistry.

2.5.6. Technician III (AFSCME 29) 2.5.6.1. The Laboratory Technician III must have completed an approved technical/vocational laboratory technician training program or one year (2 semesters or 3 trimesters) of college chemistry.

2.5.7. Technician II (AFSCME 27) 2.5.7.1. The Laboratory Technician II must have completed a technical/vocational Laboratory Technician training program or one year (2 semesters or 3 trimesters) of college chemistry.

2.5.8. Technician I (AFSCME 26) 2.5.8.1. The Laboratory Technician I must have completed a technical/vocational Laboratory Technician training program or one year (2 semesters or 3 trimesters) of college chemistry.

2.5.9. Laboratory Assistant (AFSCME 24) QA_MANUAL_2 Page 8 of 42

2.5.9.1. The Laboratory Assistant must have a high school diploma or equivalent, and be able to lift/carry fifty pounds.

3. Laboratory Personnel Training (2099, Section C and D and E2) 3.1. All results reported by the laboratory are done by certified analysts. Data produced by analysts while in the process of obtaining required training are acceptable only when reviewed and verified by a certified analyst. Certification consists of laboratory personnel receiving on the job training from certified analysts and completing a Demonstration of Capability (DOC) for each analytical procedure they are assigned. Initial and continued demonstration of laboratory capability is performed, verified, and documented to ensure analysts are capable of meeting performance criteria. The attendance at training courses or workshops for specific equipment, analytical techniques or laboratory procedures is documented in personnel files.

3.2. Certification of Analysts 3.2.1. The training in analytical methods and the laboratory certification procedure is structured into three phases. The duration of time for each phase is dependent on the prior experience of the trainee and the complexity of the analysis or instrumentation.

3.2.2. PHASE I- Observation 3.2.2.1. Trainee: Read the Standard Operating Procedure (SOP) and any applicable background material. Observe the procedure as it is performed. Ask questions as necessary to gain a basic understanding of the analysis.

3.2.2.2. Trainer: Perform the analysis in the presence of the trainee. Describe every step in the procedure and answer questions that arise. Inform the trainee of good laboratory practices, safety precautions, critical steps, customer service needs, and quality control requirements.

3.2.3. PHASE II- Performing Analysis 3.2.3.1. Trainee: Perform the SOP to successfully complete the analysis. Ask questions as necessary to gain an in depth understanding of the analysis.

3.2.3.2. Trainer: Observe the new analyst performing the analysis, answer any questions and provide direction as necessary. It is important for the trainer to ensure all procedures are performed safely and according to the SOP and verify results.

3.2.4. PHASE III: Demonstration and Documentation of Analyst Certification 3.2.4.1. Trainee: 3.2.4.1.1. Upon completion of training, the newly trained employee must demonstrate their capability prior to generating reportable results. The demonstration of capability requires the trainee to analyze standard(s) as described in the SOP. If the standards are not described in the SOP, an analyst must analyze four (4) mid-level standards spiked at the concentration of the calibration check standard. The standards must meet the acceptance criteria listed in the respective SOP. 3.2.4.2. Trainer: 3.2.4.2.1. Must ensure that Metropolitan Council employees in training, document their result(s) on the “Demonstration of Capability for Analyst Certification” document found in Appendix A (for chemistry) or Appendix B (for Microbiology). Failure to demonstrate and document the capability of an analyst may result in suspension of certification for that field of testing.

3.2.4.3. The trainee is required to document their performance evaluation results. The “Demonstration of Capability for Analyst Certification” document must be validated by a certified analyst. The analyst is considered certified, for the named analyte/method, as of the date signed by the quality assurance officer. Once the document has the required signatures, a copy of the document shall be returned to the respective section manager and the “Analyst Certification Database” is updated (Appendix F).

3.2.4.4. Record of analyst certification will be kept as hardcopies in the quality assurance office.

The quality assurance officer and/or his/her designee maintain the electronic Analyst Certification Database. The files are updated as analysts are certified or re-certified.

3.3. Continuing Analyst Certification (2099 E3a) QA_MANUAL_2 Page 9 of 42

3.3.1. The laboratory staff maintains certification by analysis of blind QC samples. The analytes are purchased through an EPA approved provider and the concentrations are unknown or blind to the analyst at the time of testing.

3.3.1.1. Continual Analyst Certification Procedure 3.3.1.1.1. Each primary analyst receives a PET-QC sample used to assess continued certification for each analyst. The PET-QC samples will be placed in the sample once per year to provide for the analysis of laboratory parameters. Please refer to section 3.3 “Analyst Capability QA Program” for an explanation of each type of quality assurance testing and requirements for primary and secondary analysts.

3.3.1.2. Failure of Continued Analyst Certification 3.3.1.2.1. In the event a primary or secondary analyst fails certification a Corrective Action Form (CAF) needs to be filed with the quality assurance team. The quality assurance officer or his/her designee decides the course of action. First there will be an investigation into the methodologies, the analyst, and analytical instrumentation. If determined necessary the primary or secondary analyst will then perform a new PETQC sample or repeat Phase III: Demonstration and Documentation of Analyst Certification. In the event the analyst has two failures in a row, a CAF is filed and the quality assurance team will investigate and document the incident. The analyst is then required to re-demonstrate capability by repeating a PT or Phase III demonstration. The Analyst Certification Database files are updated. Analyst Capability Quality Assurance Program 3.3.1.3. Annual PET-QC Testing (single blind) 3.3.1.3.1. Once per year PET-QC testing samples are analyzed to provide a quantitative assessment of instrumentation, analysts, and methodologies at a specific point-intime. The PET-QC quality assurance program is not part of the laboratory certification program (4740.2010 to 4740.2040) and parameters analyzed are based on the discretion of the quality assurance team. The primary analyst is to analyze one PET-QC samples a year. If more than one primary analyst exists for an analysis, multiple PET-QA (with various lot numbers when available) samples will be purchased and distributed. The secondary analyst shall analyze one sample to maintain certification. The PET-QC samples are purchased from EPA approved providers and arrive with results and acceptable ranges know to the quality assurance team. The results and acceptable ranges are under no circumstances divulged to analysts until results are reported and authorized in LIMS. 3.3.1.4. Annual EPA-DMR-Quality Assurance Proficiency Testing (PT) 3.3.1.4.1. Annual EPA-DMR-QA samples are analyzed as a requirement for NPDES permittees and for the MDH certification. The EPA-DMR-QA samples are purchased from an EPA approved provider. The EPA sends out notices to designated NPDES permittees, which lists the mandatory chemical and Whole Effluent Tests (WET) sample sets. All samples are prepared by the Quality Assurance Officer or their designee and inserted into the sample stream. Residual chlorine and pH samples are prepared and hand delivered to regional facilities. All results are tabulated within LIMS and reported to the approved provider for evaluation before the study closing date. Refer to section 6.0 “Proficiency Testing” for more information and requirements.

3.4. Identity Certification (2099 Section F) 3.4.1. Quality assurance maintains initials and signatures of employees on file. All paper records are initialed by the responsible analyst. Most laboratory records are maintained within a computerized Laboratory Information Management System (LIMS). Only authorized personnel may access the LIMS and make changes in the information. Control over LIMS access is maintained by access passwords and password policies. All entries in the LIMS are linked to the QA_MANUAL_2 Page 10 of 42

logged-in identity and a date/time stamp. In addition, all changes to analytical results are tracked by an independent audit trail system.

3.4.2. The integrity of laboratory data, result modifications, and data authorization is based on the use and protection of passwords. Absolute certainty is necessary to ensure the identity of the analyst within the LIMS system. As a result, it is absolutely forbidden to share passwords and computer user space with other analysts, managers, and/or information technologists.

4. Standard Operating Procedures (4740.2065) 4.1. Requirements 4.1.1. The following section identifies the laboratoryâ&#x20AC;&#x2122;s policies for establishing and maintaining all standard operating procedures for all active certified and non-certified analyses. A table of all active analyses is located in Appendix E. 4.1.2. Certified tests are referenced from the list of approved references located in 40 CFR Part 136.3 Table 1B. These reference methods are rewritten as in-house procedures, or Standard Operating Procedures (SOPs), to reflect practice in the laboratory. SOPs are available to each analyst for training and reference purposes. Alternate Test Procedures (ATP) may be applied for through the Environmental Protection Agency (EPA) when there are allowed deviations from the reference method or when a reference is not available in the CFR. 4.1.3. Analysts are required to review SOPs yearly. Any proposed changes must be provided to the section manager and approved by the QC Officer prior to implementation.

4.2. Format of Analytical Procedures (4740.2065 Subpart 3 and 8) 4.2.1. SOP Page Layout: 4.2.1.1. All margins will be set at 0.5â&#x20AC;?.

4.2.1.2. All SOPs will be in a one column format unless otherwise agreed upon by Quality Assurance.

4.2.1.3. The first page of all SOPs is the cover page consisting of Metropolitan Councilâ&#x20AC;&#x2122;s name and logo, header, and figure of merit table (if applicable).

4.2.2. Header: 4.2.2.1. SOP Version: 4.2.2.1.1. All SOPs are given a unique naming convention for identification. This convention identifies the SOP as well as the revision number of the SOP (i.e. SOP_NAME_1).

4.2.2.2. Implementation Date: 4.2.2.2.1. The implementation date is the date by which a test will be logged-in under a new SOP version.

4.2.2.2.2. The implementation date is an agreed upon date established by the section manager and Quality Assurance after all modifications to a SOP have been accepted as final.

4.2.2.2.3. Section managers are responsible for notifying staff of modifications made to a new/updated SOP. 4.2.2.2.4. All staff are responsible for following a SOP in its entirety beginning on the date of implementation.

4.2.2.2.4.1. Quality Assurance will work with section managers to determine what actions need to be taken to identify samples logged-in under the previous revision and analyzed under a new revision.

4.2.2.3. LIMS Analysis IDs: 4.2.2.3.1. All LIMS IDs associated with a SOP will be listed in the header.

4.2.2.4. Figures of Merit Table: 4.2.2.4.1. The following figures of merit, if applicable, will be listed in a table on the first page in all SOPs: 4.2.2.4.1.1. Applicable Range 4.2.2.4.1.2. Method Detection Limit and Date Performed 4.2.2.4.1.3. Reporting Limit 4.2.2.4.1.4. Preservation 4.2.2.4.1.5. Holding Time QA_MANUAL_2 Page 11 of 42

4.2.2.5. Page Numbers 4.2.2.5.1. The SOP naming convention along with total page numbers must be listed at the bottom of the document. This assures that all pages are present when following a SOP. 4.2.3. SOP Sections 4.2.3.1. Section 1 –Reference 4.2.3.1.1. When applicable, the current issue of 40 CFR Part 136 should be listed.

4.2.3.1.2. Reference Method 4.2.3.2. Section 2 – Application 4.2.3.2.1. Sample matrices 4.2.3.2.2. Applicable range, reporting limit 4.2.3.3. Section 3 – Summary of Method 4.2.3.3.1. Short description of analysis and deviations to a reference method 4.2.3.4. Section 4 – Interferences 4.2.3.4.1. List interferences and how to eliminate them if possible.

4.2.3.5. Section 5 – Apparatus 4.2.3.5.1. Instrument/Equipment 4.2.3.5.2. Labware 4.2.3.6. Section 6 – Chemicals and Reagents 4.2.3.6.1. List of all chemicals used for analysis, including manufacturer/vendor, concentration, purity, and Synergen number (when available).

4.2.3.6.2. Chemical tracking criteria (See Section 8.0 Standards, Reagents and Bacteriological Media) 4.2.3.6.3. Procedure for preparing all reagents 4.2.3.6.3.1. Reagent tracking criteria (See Section 8.0 Standards, Reagents and Bacteriological Media) 4.2.3.7. Section 7 – Safety 4.2.3.7.1. Identify where safety precautions should be observed.

4.2.3.8. Section 8 – Procedure 4.2.3.8.1. Identify all steps that need to be carried out in order to generate a result and report data to the LIMS. 4.2.3.9. Section 9 – Short Hand Procedure 4.2.3.9.1. Short Hand Procedure is no longer used and must be listed as “None” 4.2.3.10. Section 10 – Calculations 4.2.3.10.1. Equations for all calculations required in the reference method 4.2.3.11. Section 11 – Quality Control 4.2.3.11.1. List of quality control parameters required criteria for each method including but not limited to: blanks, reporting limit verification, laboratory control sample (LCS), quality control sample (QCS), matrix spike, matrix spike duplicate, relative percent difference, etc. along with frequency of analysis and corrective action for any failed QC. 4.2.3.11.2. For all certified tests, quality control parameter requirements must be followed in accordance with the reference method and any additional quality control parameters required by TNI regulations.

4.2.3.11.3. Additional quality control paramenters, for internal use only, may be added at the discretion of the section manager but must be approved by Quality Assurance prior to implementation.

4.2.3.12. Section 12 – Other References 4.2.3.12.1. Any other reference than those listed in Section 1 4.2.3.13. Section 13 – Appendix 4.2.3.13.1. Additional material needed to analyze/review data 4.2.4. SOP Development: QA_MANUAL_2 Page 12 of 42

4.2.4.1. Existing SOPs may require modifications for the following reasons: editorial changes, changes due to yearly review, new instrumentation, or the inability to perform a SOP as written.

4.2.4.1.1. Modification to a SOP can be requested by the section manager and/or by Quality Assurance.

4.2.4.1.2. The decision to modify any SOP will be made at the discretion of Quality Assurance and/or the Laboratory Manager.

4.2.4.1.3. If Quality Assurance deems it necessary to modify a SOP, creating a new revision number will be determined on a case by case basis. At minimum a new implementation date must be used to document appropriate changes.

4.2.4.1.4. The following requirements, if applicable, must be completed and approved by Quality Assurance prior to implementation: 4.2.4.1.4.1. The requirements include but are not limited to MDLs, SOP update, QC elements that are required by a reference, analytical/support equipment/chemical logbooks, DOC, and ample time for analysts to review all updates and order appropriate standards.

4.2.4.1.4.2. Quality Assurance will be responsible for summarizing changes to a SOP. This summary will be located in the â&#x20AC;&#x2DC;Descriptionâ&#x20AC;&#x2122; field in the Analysis Definition Entry Screen in the LIMS. 4.2.4.1.5. A copy of a standard operating procedure, for certified analyses, must be sent to the Minnesota Department of Health within 30 days after the effective date of the revision.

4.2.4.2. A new SOP may be generated due to the needs of a client, regulatory purposes, new instrument technology or laboratory needs.

4.2.4.3. Quality Assurance must be notified at the time of the request and will work with the associated parties to develop a work plan to complete all necessary requirements.

5. Laboratory Quality Assurance Practices and Objectives (4740.2080, 4740.2100, and 4740.2110) 5.1. Scope and Objectives (2080/2100 and 2110, Subpart 1) 5.1.1. All of the following criteria are incorporated into each SOP based on requirements set forth by the approved method.

5.1.2. All quality control requirements listed in the Quality Control section of the SOP must be followed.

5.2. Quality Control Criteria for Chemistry (2100) 5.2.1. Method Blanks (Subpart 2) 5.2.1.1. A blank is used to assess contamination from the laboratory.

5.2.1.2. Blanks must be processed with and under the same conditions as the associated samples to include all steps in the analytical procedure.

5.2.1.3. Criteria for blank frequency, acceptability, and corrective action for failed blanks are listed in the Quality Control section of each SOP. 5.2.2. Laboratory Control Sample (Subpart 3) 5.2.2.1. Criteria for laboratory control sample frequency, acceptability, and corrective action for failed laboratory control samples are listed in the Quality Control section of each SOP. 5.2.3. Quality Control Sample (4740.2093 Subpart 3 C) 5.2.3.1. A quality control sample is a second source sample used to verify purity and preparation of the calibration standards.

5.2.3.2. Criteria for quality control sample frequency, acceptability, and corrective action for failed quality control samples are listed in the Quality Control section of each SOP. 5.2.4. Matrix Spike and Matrix Spike Duplicate (Subpart 4) 5.2.4.1. A matrix spike/matrix spike duplicate pair is analyzed to determine the ability to recover the known analyte or compound in that sample matrix.

5.2.4.2. Matrix spikes and matrix spike duplicates are not required for analyses where there is no spiking solution available.

QA_MANUAL_2 Page 13 of 42

5.2.4.3. Analytical procedures requiring a matrix spike and matrix spike duplicate have criteria for frequency, acceptability and corrective action for failed spikes listed in the Quality Control section of each SOP. 5.2.5. Surrogate Spikes (Subpart 5) 5.2.5.1. Surrogate spiking applies only to the analysis of organic compounds.

5.2.5.2. Surrogates are comparable to the analytes of interest in chemical composition and assume to behave similarly in the analytical process.

5.2.5.3. Surrogate compounds must be added to all samples, standards and blanks prior to sample preparation or extraction.

5.2.5.4. Criteria for surrogate recovery, acceptability and tabulation are listed in the Quality Control section of each SOP. 5.2.6. Internal Standards (Subpart 6) 5.2.6.1. Internal standards (ISTD) are pure analytes similar in analytical behavior to the compounds of interest. ISTDs are added to a sample in known amounts and used to measure the relative responses of other method analytes and surrogates in solution.

5.2.6.2. Internal standards are selected so that the measurement of the internal standard assumes minimal affect by method and matrix interferences.

5.2.6.3. Analytical procedures requiring the use of internal standards are added to all samples, standards and blanks prior to analysis.

5.2.6.4. For organic compounds, surrogate recoveries and reporting limit verifications are used as determinates of acceptable internal standard area counts.

5.2.6.5. For metal analysis, acceptable internal standard responses are based on response deviation from the original response of the calibration blank.

5.2.7. Detection Limits (Subpart 7) 5.2.7.1. Method Detection Limit (MDL): 5.2.7.1.1. The method detection limit (MDL) is the minimum concentration of a substance that can be measured and reported with 99% confidence that the analyte concentration is greater than zero and is determined from analysis of a sample in a given matrix containing the analyte.

5.2.7.1.2. MDLs are not applicable to analyses where there is no spiking solution available.

5.2.7.1.3. The laboratory uses the EPA Procedure for determining MDLs is located in 40 CFR Appendix B to Part 136.

5.2.7.1.4. All MDLs are performed by spiking reagent water with the analyte of interest.

5.2.7.1.5. MDLs will be determined at the frequency specified in the approved reference method or when changes occur to the analytical system that will impact the MDL. 5.2.7.2. Detection Limit (DL): 5.2.7.2.1. All analytical methods employed by the MCES Laboratory will have a numerical Detection Limit (DL) assigned, except where such a limit would be technically or otherwise inappropriate, as determined by the Quality Assurance Officer. This DL will be used to censor all analytical results for the associated analytical method: all analytical results below the assigned limit will be reported as less than that numerical value (i.e., <{value}).

5.2.7.2.2. These DL values will be assigned by the QAO, taking into account pertinent factors such as routinely achievable EPA MDL’s, client needs and typical matrices, and method or regulatory based requirements. Generally, the values assigned are intended to be routinely achievable, that is, they will be no larger than necessary to assure that ongoing, determined EPA MDL’s will be below the DL’s with a high degree of confidence.

As circumstances dictate (new operators, new equipment, passage of time, or other potentially significant changes occur), EPA MDL’s will be determined to verify that the DL’s are still valid. The results of EPA MDL’s will be recorded and catalogued in the Laboratory’s LIMS. QA_MANUAL_2 Page 14 of 42

5.2.8. Reporting Limits (Subpart 8) 5.2.8.1. All analytical methods employed by the MCES Laboratory will have a numerical Reporting Limit (RL) assigned, except where such a limit would be technically or otherwise inappropriate, as determined by the QAO. This RL will be used to censor all analytical results for the associated analytical method: all analytical results below the RL assigned limit but above the DL, will be reported with a tilde qualifier (i.e., ~{value}).

RLâ&#x20AC;&#x2122;s are based on method specific accuracy/recovery requirements. When these requirements are not specified, the RL must be validated, with a standard at or below the reporting limit, each time the instrument is calibrated. The RL standard must fall within plus or minus 40% of the true value.

5.2.8.2. Criteria for reporting limit verification frequency, acceptability, and corrective action for failed reporting limit verifications are listed in the Quality Control section of each SOP. 5.2.9. Selectivity (Subpart 9) 5.2.9.1. Selectivity only applies to volatile organic compounds and other organic compounds.

5.2.9.2. Selectivity utilizes absolute retention time and relative retention time to identify components in chromatographic analyses and to evaluate the effectiveness of a chromatographic medium to separate constituents.

5.2.9.3. For the GC-MS, compound identification is confirmed by comparing retention times and ions ratios to authentic standards.

5.2.9.4. For GC systems, compound identification is confirmed by using a dual column.

5.2.10. Manual Integrations (Subpart 10) 5.2.10.1. Manual integrations performed on GC and GC/MS systems are identified and calculated by the software.

5.2.11. Uncertainty Measurement 5.2.11.1. All results meet specifications of the reference method unless otherwise specified.

Additional uncertainty estimates for certified parameters have been performed by the laboratory and may be available upon request.

5.3. Quality Control Criteria for Bacteriology (4740.2110) 5.3.1. Sterility Checks and Blanks (Subpart 2) 5.3.1.1. A blank must be analyzed for each lot of prepared, ready-to-use media, including chromofluorogenic reagent, and for each lot of media prepared in the laboratory.

5.3.1.2. One beginning and one ending sterility check must be performed for each laboratorysterilized filtration unit used in a filtration series.

5.3.1.2.1. The filtration series is considered ended when more than 30 minutes elapse between successive filtrations.

5.3.1.2.2. During a filtration series, filter funnels must be rinsed with three 20 to 30 mL portions of sterile rinse water after each sample filtration.

5.3.1.3. For pour-plate technique, one sterility blank of the media must be made by pouring one uninoculated plate for each lot of prepared, ready-to-use media and one for each lot of media prepared in the laboratory.

5.3.1.4. Sterility checks on sample containers must be performed on at least one container for each lot of purchased, presterilized containers. For containers sterilized in the laboratory, a sterility check must be performed on one container per sterilized batch using nonselective growth media.

5.3.1.5. A sterility check must be performed on each batch of dilution water prepared in the laboratory and on each batch of pre-prepared, ready-to-use dilution water using nonselective growth media.

5.3.1.6. At least one filter from each new lot of membrane filters must be checked for sterility using nonselective growth media.

5.3.2. Positive Controls (Subpart 3) 5.3.2.1. Each pre-prepared, ready-to-use lot of media, including chromofluorogenic reagent, and each lot of media prepared in the laboratory must be tested with at least one pure culture of QA_MANUAL_2 Page 15 of 42

a microorganism known to elicit a positive reaction. This must be done before first use of each lot of media.

5.3.3. Negative Controls (Subpart 4) 5.3.3.1. Each pre-prepared, ready-to-use lot of selective media, including chromofluorogenic reagent, and each lot of selective media prepared in the laboratory must be analyzed with one or more known negative culture controls, that is, nontarget microorganisms that should not grow on the test media, as appropriate to the method. This must be done before first use of each lot of media.

5.3.4. Test Variability (Subpart 5) 5.3.4.1. For test methods that specify colony counts, such as methods using membrane filters or plated media, duplicate counts must be performed monthly on at least one positive sample for each month that the test is performed. With respect to this test for variability, if the laboratory has two or more analysts, each analyst must count typical colonies on the same plate and counts must be within ten percent difference between analysts to be acceptable.

In a laboratory with only one microbiology analyst, the same plate must be counted twice by the analyst, with no more than five percent difference between the counts.

5.3.5. Method Evaluation (Subpart 6) 5.3.5.1. A laboratory must demonstrate proficiency with the test method before first use, by comparison to a method already approved for use in the laboratory, by analyzing a minimum of ten spiked samples whose matrix is representative of those normally submitted to the laboratory, or by analyzing and passing one proficiency test series provided by an approved proficiency sample provider. The laboratory must maintain documentation of the proficiency demonstration as long as the method is in use and for at least five years after the date of last use.

5.3.6. Test Performance (Subpart 7) 5.3.6.1. To ensure that analytical results are accurate, a laboratory must confirm a target organism specified in the method.

5.3.7. Quality of Standards, Reagent and Media (Subpart 8) 5.3.7.1. Culture media may be prepared from commercial dehydrated powders or may be purchased ready to use, unless otherwise indicated in the approved method. Media may be prepared by the laboratory from basic ingredients when commercial media are not available or when it can be demonstrated that commercial media do not provide adequate results. Media prepared by the laboratory from basic ingredients must be tested for performance, such as for selectivity, sensitivity, sterility, growth promotion, and growth inhibition, before first use. Detailed testing criteria information must be defined in the laboratory's standard operating procedures manual or quality assurance manual.

5.3.7.2. Reagents, commercial dehydrated powders, and media must be used within the shelf life of the product. The specifications of the reagent, powder, or media must be documented according to the laboratory's quality assurance manual.

5.3.7.3. Distilled water, deionized water, or reverse-osmosis produced water that is free from bactericidal and inhibitory substances must be used in the preparation of media, solutions, and buffers. The quality of the water must be monitored for chlorine residual, specific conductance, and heterotrophic bacteria plate count monthly, when in use; when maintenance is performed on the water treatment system; or at startup after a period of disuse longer than one month. Analysis for metals and the bacteriological water quality test, to determine the presence of toxic agents or growth promoting substances, must be performed annually. Results of these analyses must meet the specifications of the required method and records of analyses must be maintained for five years. Laboratories that can supply documentation to show that their water source meets the criteria, as specified by the method, for ASTM or NCCL Type I or Type II reagent water and is free of bacteria that can grow under these test conditions are exempt from performing the bacteriological water quality test.

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5.3.7.4. Media, solutions, and reagents must be prepared, used, and stored according to a documented procedure following the manufacturer's instructions or the test method.

Documentation for media prepared in the laboratory must include the date of preparation, preparer's initials, type and amount of media prepared, manufacturer and lot number, final pH of the media, and expiration date.

5.3.7.5. Documentation for media purchased pre-prepared and ready-to-use must include the manufacturer, lot number, type and amount of media received, date of receipt, expiration date of the media, and the verification pH of the liquid.

5.3.8. Selectivity (Subpart 9) 5.3.8.1. To ensure identity and traceability, reference cultures used for positive and negative controls must be obtained from a recognized national collection or organization.

5.3.8.2. Microorganisms may be single-use preparations or cultures maintained by documented procedures that demonstrate the continued purity and viability of the organism.

5.3.8.3. Reference cultures may be revived, if freeze-dried, or transferred from slants and subcultured once to provide reference stocks. The reference stocks must be preserved by a technique that maintains the characteristics of the strains. Reference stocks must be used to prepare working stocks for routine work. If reference stocks have been thawed, they must not be refrozen and reused.

5.3.8.4. Working stocks must not be cultured sequentially more than five times and must not be subcultured to replace reference stocks.

5.3.9. Temperature Measuring Devices (Subpart 10) 5.3.9.1. Temperature measuring devices such as liquid-in-glass thermometers, thermocouples, and platinum resistance thermometers used in incubators, autoclaves, and other equipment must be of the appropriate quality to meet specifications in the test method. The gradation of the temperature measuring devices must be appropriate for the required accuracy of measurement and the devices must be calibrated to national or international standards for temperature. All measurements must be recorded.

5.3.10. Autoclaves (Subpart 11) 5.3.10.1. The performance of each autoclave must be evaluated initially by establishing its functional properties and performance, for example heat distribution characteristics with respect to typical uses. Autoclaves must meet specified temperature tolerances. Pressure cookers must not be used for sterilization of growth media.

5.3.10.2. Demonstration of sterilization temperature must be provided by use of a continuous temperature recording device or by use of a maximum registering thermometer with every cycle. Appropriate biological indicators must be used once per month to determine effective sterilization. Temperature-sensitive tape must be used with the contents of each autoclave run to indicate that the autoclave contents have been processed.

5.3.10.3. Records of autoclave operations must be maintained for every cycle. Records must include: date, contents, maximum temperature reached, pressure, time in sterilization mode, total run time, which may be recorded as time in and time out, and operator's initials.

5.3.10.4. Autoclave maintenance, either internally or by service contract, must be performed annually and must include a pressure check and calibration of the temperature device.

Records of the maintenance must be maintained in equipment logs.

5.3.10.5. The autoclave's mechanical timing device must be checked quarterly against a stopwatch and the actual time elapsed must be documented.

5.3.11. Incubators, Water Baths and Ovens (Subpart 13) 5.3.11.1. The stability and uniformity of temperature distribution and the time required after test sample addition to reestablish equilibrium conditions in incubators and water baths must be documented. Temperature of incubators and water baths must be documented twice daily, at least four hours apart, on each day of use.

5.3.12. Labware, Glassware and Plasticware (Subpart 14) QA_MANUAL_2 Page 17 of 42

5.3.12.1. A laboratory must have a documented procedure for washing labware, if applicable.

Detergents designed for laboratory use must be used.

5.3.12.2. Glassware must be made of borosilicate or other noncorrosive material, free of chips and cracks, and have readable measurement marks.

5.3.12.3. Labware that is washed and reused must be tested for possible presence of residues that may inhibit or promote growth of microorganisms by performing the inhibitory residue test annually and each time the laboratory changes the lot of detergent or washing procedures.

5.3.12.4. Washed labware must be tested at least once daily, each day of washing, for possible acid or alkaline residue by testing at least one piece of labware with a suitable pH indicator such as bromothymol blue. Records of tests must be maintained.

6. Proficiency Testing (PT) (4740.2070 and TNI Standard V1M1) 6.1. Proficiency Testing Scope 6.1.1. To obtain initial accreditation, the laboratory shall successfully analyze two proficiency testing samples for each field of proficiency testing for which it seeks accreditation. (TNI Standard V1M1: 4.1.1) 6.1.2. The laboratory shall obtain PT samples from an accredited TNI-compliant PT provider. (TNI Standard V1M1: 4.1.2) 6.1.3. In order to maintain accreditation, the laboratory will analyze at least two PT samples per calendar year for each field of testing. (TNI Standard V1M1: 4.2.1a) 6.1.4. The analysis dates of successive PT samples for the same accreditation Field of Proficiency Testing shall be at least five months apart and no longer than seven months apart unless the PT sample is being used for corrective action. (TNI Standard V1M1: 4.2.1a) 6.1.5. In order to maintain accreditation, the laboratory will maintain a history of at least two successful performances out of the most recent three attempts for each requested field of proficiency testing. (TNI Standard V1M1: 4.2.1b) 6.1.6. The laboratory will participate in the annual Discharge Monitoring Report Quality Assurance Study (DMR-QA) that is designed to evaluate the entire process used to routinely report results in Discharge Monitoring Reports.

6.1.6.1. The DMR-QA study is mandatory for permit holders under the Clean Water Actâ&#x20AC;&#x2122;s National Pollutant Discharge Elimination System (NPDES).

6.1.6.2. The study requirements are outlined in the DMR-QA Study Announcement packet which is mailed to NPDES permittees in the spring of each year.

6.1.6.3. PT results used for accreditation purposes may be acceptable to use for the DMR-QA Study if they meet the criteria as outlined in the DMR-QA Study Announcement packet.

6.2. Laboratory Testing of PT Samples and Reporting 6.2.1. Analysts are required to analyze all PT samples in the same manner as routine samples, employing all quality control requirements listed in the SOP. 6.2.2. The laboratory shall report PT results according to the reporting requirements listed in the TNI Standard. (TNI Standard V1M1: 5.2) 6.2.3. The lab will authorize the PT Provider to release all accreditation and remediation results and acceptable/not acceptable status directly to the Minnesota Department of Health, our Primary Accrediting Authority. (TNI Standard V1M1: 5.2.3) 6.2.4. The laboratory will maintain copies of records, including bench sheets, instrument strip charts, data calculations, and data reports, for five years. (TNI Standard V1M1: 5.3) 6.2.5. When the result of any reported field of testing is unacceptable, the laboratory must within 30 days after receiving the notification of results submit written documentation to MDH indicating corrective actions planned and taken. (4740.2070 Subpart 9) 6.2.6. The lab may elect to participate in supplemental PT studies when the lab desires to add field(s) of proficiency testing to their scope or when the lab fails an initial or continuing PT study and wishes to re-establish its history of successful performance. (TNI Standard V1M1: 6.1a) 6.2.7. There must be at least 15 calendar days between the analysis dates of successive PT samples for the same field of proficiency testing. (TNI Standard V1M1: 6.1b) QA_MANUAL_2 Page 18 of 42

7. Laboratory Sample Handling, Receipt and Acceptance (4740.2087) 7.1. Sample Handling, Sample Receipt Protocols and Preservation (Subpart 1) 7.1.1. The laboratory utilizes a Laboratory Information Management System (LIMS) to distinguish each individual sample and/or test with a unique identification.

7.1.1.1. The LIMS consists of four main tables of operation: Job, Sample, Test and Result.

7.1.1.1.1. There can be 1 or more samples in a job, 1 or more tests for a sample, and 1 or more results for a test.

7.1.1.2. Job, Sample and Test are unique identifiers that can never be repeated based on the design of the software. This also implies that Results will be unique.

7.1.1.3. Once information has been entered in the LIMS it has been “committed,” meaning it is archived and can be retrieved at any time.

7.1.1.4. “Projects” are set-up on an individual client basis. Clients submit to the laboratory a list of sampling locations, sample types, and parameters to be analyzed. They will then be given a unique project number that will be used throughout the lifetime of the client/laboratory relationship.

7.1.2. NPDES Samples: 7.1.2.1. When samples arrive to the laboratory, a Sample Missing Sheet is filled out to distinguish which samples are present, whether the cooling process had begun and whether NPDES samples requiring preservation have been preserved.

7.1.2.2. All NPDES samples requiring preservation are preserved within 15 minutes of sampling.

7.1.2.2.1. The pH of these samples are tested by placing a small amount of sample onto a pH paper strip.

7.1.2.2.1.1. If the sample has been properly preserved, it is mark that it is acceptable on the Sample Missing Sheet (SMS).

7.1.2.2.1.2. If the sample was not preserved properly, added more preservative and check the pH again. Do this until the sample is at the acceptable pH. 7.1.2.2.1.2.1. On the SMS, mark that the sample was not acceptable and note any additional comments/irregularities in the Notes section. Notify section manager and comment in the LIMS. 7.1.2.3. All SMS are filed with the current month’s Daily Monitoring Reports (DMR).

7.1.2.4. Samples are then transferred to a walk-in cooler for storage.

7.1.2.4.1. The cooler optimizes dual cooling units that alternate in their use to maintain reliability.

7.1.2.4.2. The walk-in cooler is continually monitored by a MUX system. The MUX system records temperature readings every second and is set-up to alert the proper personal if an issue would arise.

7.1.2.4.3. All standards are stored in separate refrigerators from all samples.

7.1.3. Non NPDES Samples: 7.1.3.1. Clients are required to submit a Sample Submission Sheet to the laboratory along with its corresponding sample.

7.1.3.2. All samples must be clearly identified and all Sample Submission Sheet completed in full.

7.1.3.2.1. Sample Submission Sheets are filed and stored for 5 years.

7.1.3.3. The following information is usually required for samples to be logged into the LIMS, but is project dependent: 7.1.3.3.1. Project Number 7.1.3.3.2. Sample Location 7.1.3.3.3. Sample Name 7.1.3.3.4. Sampling Point 7.1.3.3.5. Sampling collection date and time 7.1.3.3.6. Parameters requested 7.1.3.4. This information along with an identification number and bar code are printed onto labels designated by each field of testing.

QA_MANUAL_2 Page 19 of 42

7.1.3.5. After samples have been logged into the LIMS, they are labeled, split into appropriate testing vials/containers and preserved accordingly.

7.1.3.6. Samples are then transferred to a walk-in cooler for storage.

7.2. Sample Acceptance Policy (Subpart 3) 7.2.1. See Appendix G for the laboratory’s Sample Acceptance Policy.

8. Standards, Reagents and Bacteriological Media (4740.2089) 8.1. Logbook Documentation for Chemicals and Reagents (Subpart B): 8.1.1. Logbook Front Cover: 8.1.1.1. Title 8.1.1.2. Book number 8.1.1.3. Starting date 8.1.2. Chemical Entries: 8.1.2.1. The following must be documented in the logbook each time a new chemical is opened: 8.1.2.1.1. Identification of manufacturer/vendor 8.1.2.1.2. Certificate of Analysis/Purity retained and filed 8.1.2.1.3. Lot number 8.1.2.1.4. Date opened 8.1.2.1.5. Analyst’s initials 8.1.2.1.6. Method of preparation if not listed in SOP 8.1.2.1.7. Recommended storage if not specified in SOP 8.1.2.1.8. Expiration date (If an expiration date is not provided by the manufacturer or vendor it is not required) (TNI Standard V1M1: 5.6.4.2).

8.1.3. Reagent Entries: 8.1.3.1. The following must be documented in the logbook each time a new reagent is made: 8.1.3.1.1. Date prepared 8.1.3.1.2. Analyst’s initials 8.1.3.1.3. Reference to chemical used to prepare reagent (page, line) 8.1.3.1.4. Method of preparation if not listed in SOP 8.1.3.1.5. Recommended storage if not specified in SOP 8.1.3.1.6. Expiration date 8.1.3.1.6.1. Expiration date of a reagent cannot exceed that of its precursor(s).

8.2. Chemical and Reagent Bottle Labels (Subpart C): 8.2.1. Chemical Bottle Labels: 8.2.1.1. All chemical bottles must be labeled with unless otherwise noted in the SOP: 8.2.1.1.1. Date bottle was opened 8.2.1.1.2. Expiration date 8.2.1.1.3. Logbook Reference 8.2.2. Reagent Bottle Labels: 8.2.2.1. All reagent containers must be labeled with the following information unless otherwise noted in the SOP: 8.2.2.1.1. Name of reagent 8.2.2.1.2. Concentration/content 8.2.2.1.3. Date of preparation 8.2.2.1.4. Initials of analyst 8.2.2.1.5. Expiration date 8.2.2.1.6. Logbook reference (page, line) 9. Support Equipment Maintenance (4740.2091) 9.1. Scope of Support Equipment Maintenance (2091, Section 1.0 Subpart 1 Section A-B and Section 2.0) 9.1.1. Support equipment is necessary for laboratory operations and results are dependent upon their accuracy. The support equipment is properly maintained, inspected, and cleaned to ensure that the laboratory’s instrumentation is in control. Laboratory support equipment includes, but is not QA_MANUAL_2 Page 20 of 42

limited to the following: balances, ovens, refrigerators, freezers, incubators, water baths, block digesters, temperature measuring devices, thermistors, thermal/pressure sample preparation devices, autoclaves, volumetric dispensing devices and diluter devices. Laboratory support equipment includes all devices that may not be the actual test instrument, but are necessary to support laboratory operations. Analytical instrumentation (i.e. ICPMS, GC-MS, TRAACS and GC) and their requirements for instrumentation are listed in section 10.0. All support equipment is: 9.1.1.1. Purchased to meet verified performance specifications, (2091 Subpart 2 Section C) 9.1.1.2. Operated by trained personnel with access to up-to-date instruction manuals for reference, use, and maintenance, (2091 Subpart 2 Section A) 9.1.1.3. Properly maintained, inspected, calibrated, and cleaned by the servicing company and/or the technician. Maintenance and service activities are recorded in individual logbooks, (2091, Subpart 2 Section B) 9.1.1.4. As appropriate, calibrated and maintained at least annually and are traceable to internationally based references when available, over the entire range of use. The results of such calibrations must be within the specifications required of the application for which the equipment is used or the equipment is removed from service until repaired.

(2091, Subpart 4 Section C).

9.1.1.5. QA Officer will verify that annual maintenance plans have been completed.

9.2. Support Equipment and Software Logbooks: (2091, Subpart 2 Section C) 9.2.1. Individual logbooks are maintained for each item of support equipment and software. Logbooks are essential because they chronologically document preventative maintenance, repairs, service, calibrations, cleaning, and software applications. The equipment operator is to ensure that each piece of equipment has an updated logbook located next to each instrument or see section manager. Instrument and software logbooks shall maintain a specific format for each instrument and include the following requirements: 9.2.2. Logbook Front Cover Content 9.2.2.1. The center of the logbook cover shall have a list of the following information: 9.2.2.2. Identity of the analytical piece of equipment and/or software, 9.2.2.3. The manufacturer’s name, serial number, MCES property identification number and/or designated MCES instrument code (i.e. TRAACS 1, 2, 3…)

9.2.2.4. Initial date of the individual logbook as assigned to the equipment and the date completed or removed from service.

9.2.2.5. Sequential logbook number that is assigned to each new logbook starting with number 1 and counted up for each piece of designated equipment/software.

9.2.3. Logbook Content 9.2.3.1. Support instrument logbooks contain two sections and are intended to track static and active information. All logbook entries are recorded in blue or black permanent ink. The usage of white out or obliterated entries is not permitted.

9.2.3.2. Static Information 9.2.3.2.1. The first few pages of the laboratory manual are reserved for static information that is known to be stable or non-changing. General information placed on the cover is also transferred to the first few pages of the laboratory notebook. The following is a list of recorded static information when applicable and available: 9.2.3.2.2. Equipment/software name and/or version 9.2.3.2.3. Current location within the laboratory (Bench Code System) 9.2.3.2.4. Manufacturer’s name and serial number 9.2.3.2.5. MCES Property identification number 9.2.3.2.6. Initial and final date of logbook 9.2.3.2.7. Analyses assigned 9.2.3.2.8. Designated Instrument Codes (i.e. TRAACS 1, 2, 3….)

9.2.3.2.9. Software configuration information QA_MANUAL_2 Page 21 of 42

9.2.3.2.10. Date received/ Implementation date (mm/dd/yyyy) 9.2.3.2.11. Equipment specifications 9.2.3.2.12. Manufacturer’s Manual 9.2.3.2.13. Maintenance Plan 9.2.3.2.14. Condition when received, such as new, used or reconditioned 9.2.3.3. Active Information: 9.2.3.3.1. The second portion of the logbook is used to chronologically track routine and nonroutine maintenance. All maintenance that is non-routine or requires additional service not outlined in the maintenance schedule is distinguished with a highlighter pen. The following is a list/description of the items included in the active section of the logbook: 9.2.3.3.2. Date (mm/dd/yyyy) of routine and non-routine maintenance 9.2.3.3.3. Initials/Signature 9.2.3.3.4. Nature of damage, malfunction, modification, or repair 9.2.3.3.5. Adjustments made, parts used and who made adjustments 9.2.3.3.6. Acceptance criteria/reference material verification 9.2.3.3.7. Copies of reports, results and certifications of calibrations (paste/tape copies into the logbook) 9.2.3.3.8. Due date of next calibration 9.2.3.3.9. Removal of equipment when criteria not met 9.3. Frequency of Calibration (Subpart 3) 9.3.1. All support equipment, except Class A, must be calibrated prior to first use.

9.3.2. All heating and cooling devices, where a specified temperature is required, will be verified and recorded on days of use.

9.3.3. Mechanical volumetric dispensing devices including burettes, except Class A glassware, must be checked for accuracy quarterly.

9.3.4. All glassware, including glass micro liter syringes used for calibration, must be checked for accuracy and documented before its first use in the laboratory if the glassware does not come with a certificate attesting to establish accuracy.

9.3.5. The temperature, cycle time, and pressure of each run on the autoclave must be documented by the use of appropriate chemical indicators, temperature recorders, and pressure gauges.

9.3.6. Volumetric equipment must be calibrated as follows: 9.3.6.1. Equipment such as filter funnels, bottles, non-Class A glassware, and other marked containers must be calibrated once per lot prior to first use.

9.3.6.2. Disposable pipettes and micropipette tips must be checked once per lot.

9.4. Support Equipment Maintenance Plans (Subpart 3 Section A) 9.4.1. Each piece of support equipment has a maintenance plan that includes scheduled maintenance and calibration requirements/frequency. Calibration procedures depend upon the acceptance criteria listed in the analytical method as described in the Standard Operating Procedure (SOP) for each analysis or as established in manufacturer’s literature. In the absence of any method or manufacturer’s specific calibration requirements, the individual maintenance plan shall be established and approved by Quality Assurance Officer. A list of maintenance procedures and frequencies are outlined on a routine/preventative maintenance schedule and placed in the static section of the logbook.

9.5. Documentation and Archiving of Logbooks 9.5.1. Logbooks are filed alphabetically and chronologically in the quality assurance files when the support equipment’s lifecycle is over or when the logbook is full. The logbook(s) are filed once the final entry date is recorded, the spine is labeled (name, book number and dates) and a new logbook created. The completed logbooks are maintained in the quality assurance office for a minimum of five years as per the Record Retention and Retrieval statute 4740.2097.

10. Instrument Operational Validation (4740.2093) 10.1. Scope of Instrument Validation (2093, Subpart 1 and Subpart 2 Section A-B) QA_MANUAL_2 Page 22 of 42

10.1.1. Analytical instrumentation is actual test devices used in the generation of test results. All instrumentation is: 10.1.1.1. Purchased to meet verified performance specifications, (2093 Subpart 2 Section C) 10.1.1.2. Operated by trained personnel with access to up-to-date instruction manuals for reference, use, and maintenance, (2093 Subpart 2 Section A) 10.1.1.3. Properly maintained, inspected, calibrated, and cleaned by the servicing company and/or the technician. Maintenance and service activities are recorded in individual logbooks, (2093, Subpart 2 Section B) 10.1.1.4. As appropriate, calibrated and maintained at least annually and are traceable to internationally based references when available, over the entire range of use. The results of such calibrations must be within the specifications required of the application for which the equipment is used or the equipment is removed from service until repaired.

(2093, Subpart 2 Section B).

10.1.1.5. QA Officer will verify that annual maintenance plans are completed.

10.2. Analytical Instrumentation and Software Logbooks: (2093, Subpart 2 Section C) 10.2.1. Individual logbooks are maintained for each item of analytical instrumentation and software.

Logbooks are essential because they chronologically document preventative maintenance, repairs, service, calibrations, cleaning, and software applications. The equipment operator is to ensure that each piece of equipment has an updated logbook located next to each instrument or see section manager. Instrument and software logbooks shall maintain a specific format for each instrument and include the following requirements: 10.2.1.1. Logbook Front Cover Content 10.2.1.1.1. The center of the logbook cover shall have a list of the following information: 10.2.1.1.2. Identity of the analytical piece of equipment and/or software, 10.2.1.1.3. The manufacturer’s name, serial number, MCES property identification number and/or designated MCES instrument code (i.e. TRAACS 1, 2, 3…)

10.2.1.1.4. Initial date of the individual logbook as assigned to the equipment and the date completed or removed from service.

10.2.1.2. Logbook Content 10.2.1.2.1. Analytical instrument logbooks contain two sections and are intended to track static and active information. All logbook entries are recorded in blue or black permanent ink. The usage of white out or obliterated entries is not permitted.

10.2.1.2.2. Static Information 10.2.1.2.3. The first few pages of the laboratory manual are reserved for static information that is known to be stable or non-changing. General information placed on the cover is also transferred to the first few pages of the laboratory notebook. The following is a list of when applicable and available: 10.2.1.2.4. Equipment/software name and/or version 10.2.1.2.5. Current location within the laboratory (Bench Code System) 10.2.1.2.6. Manufacturer’s name and serial number 10.2.1.2.7. MCES Property identification number 10.2.1.2.8. Initial and final date of logbook 10.2.1.2.9. Analyses assigned 10.2.1.2.10. Designated MCES Instrument Codes (i.e. TRAACS 1, 2, 3….)

10.2.1.2.11. Software configuration information 10.2.1.2.12. Date received/ Implementation date (mm/dd/yyyy) 10.2.1.2.13. Equipment specifications 10.2.1.2.14. Manufacturer’s Manual 10.2.1.2.15. Maintenance Plan 10.2.1.2.16. Condition when received such as new, used, or reconditioned 10.2.1.2.17. Active Information QA_MANUAL_2 Page 23 of 42

10.2.1.2.18. The second portion of the logbook is used to chronologically track routine and nonroutine maintenance. All maintenance that is non-routine or requires additional service not outlined in the maintenance schedule is distinguished with a highlighter pen. The following is a list/description of the items included in the active section of the logbook: 10.2.1.2.19. Date (mm/dd/yyyy) of routine and non-routine maintenance 10.2.1.2.20. Initials/Signature 10.2.1.2.21. Nature of damage, malfunction, modification, or repair 10.2.1.2.22. Adjustments made, parts used and who made adjustments 10.2.1.2.23. Acceptance criteria/reference material verification Copies of reports, results and certifications of calibrations (paste/tape copies into the logbook) 10.2.1.2.24. Due date of next calibration 10.2.1.2.25. Removal of equipment when criteria not met 11. Analytical Response Calibration (4740.2093) 11.1. Analytical Response Calibration (2093, Subpart 3) 11.1.1. Calibration of an instrument is completed to convert instrument responses to concentration.

Sufficient records must be retained to allow for reconstruction of the instrument calibration.

Records should include: calibration date, approved method, instrument, analysis date, each analyte name, analystâ&#x20AC;&#x2122;s initials or signature, concentration and response, calibration curve or response factor, or unique equation or coefficient used to reduce instrument responses to concentration.

11.1.2. The most current instrument calibration must be utilized to quantitate sample results.

11.1.3. A second source standard (Quality Control Sample) purchased from an alternate manufacturer or by the same manufacturer with different lot numbers, will be used to verify all instrument calibrations (see Section 11.2.)

11.1.4. Acceptance criteria for each instrument calibration are documented in the standard operating procedure (i.e. RL, QCS, LCS, and Blank.)

11.1.5. Only sample results within the calibration range will be accepted. Any sample results greater than the calibration range will be diluted and reanalyzed to meet the established criteria. Any sample results less than the calibration range will be qualified in the LIMS with a tilde.

11.1.6. Methods employing standardization with a zero point and a single point calibration point must have the following: 11.1.6.1. Prior to sample analysis, a series of standards will be analyzed to establish a linear range.

One of the standards must be at the single point quantitation level.

11.1.6.2. The linearity must be verified at a frequency specified in the SOP. 11.1.6.3. A zero point and a single point calibration standard must be analyzed with each analytical batch.

11.1.6.4. Each analytical batch must include a standard at or below the reporting limit. This standard must be within plus or minus 40 percent of its true value (2100, Subpart 8.)

11.1.7. If the instrument calibration does not meet the requirements set forth by the acceptance criteria, appropriate action must be taken to alleviate the issue and all samples affected must be reanalyzed. If sample reanalysis is not possible, all associated data must be qualified.

11.1.8. Calibration standards must not exceed concentration limits specified by the approved method.

11.1.9. The minimum number of required calibration standards for any test is 3 (unless specified in an approved method), not including a blank (zero standard), and one of these standards must be at the reporting limit. Instruments with established methodologies and procedure demonstrating the successful use of a zero and a single point standard may be used as an exception. All SOPs contain the number of calibration standards and the acceptance criteria required for the instrument calibration.

11.1.10. The frequency of an instrument calibration is specified in each SOP. 11.2. Calibration Verification (2093, Subpart 4) QA_MANUAL_2 Page 24 of 42

11.2.1. If an instrument calibration was not established on the day of analysis, a calibration verification (Laboratory Control Sample) must be analyzed with each batch to verify the instrument calibration prior to sample analysis.

11.2.2. The frequency, acceptance criteria and correction action for calibration verifications are specified in each SOP. 12. Laboratory Sample Reporting (4740.2095) 12.1. Data Reporting 12.1.1. The laboratory utilizes LIMS for reporting all data.

12.1.2. All data must be reported according to instructions listed in the SOP. 12.1.3. Clients receive results by electronic or paper reports. In certain cases where time is limited, the client may receive verbal results prior to an electronic or paper report.

12.1.4. The laboratory is responsible for preparing all regulatory reports and has all information required to report for compliance purposes. The laboratory is therefore exempt from formal report requirements listed in Minnesota Rule 4740.2095 Subpart C. 12.2. Reporting Convention: 12.2.1. All values less than the DL will be reported as < DL. 12.2.2. All values between the DL and RL will be preceded by a tilde (~).

12.2.3. All values between the RL and the top of the calibration range will be reported as is.

12.2.4. All values > the top of the calibration range must be diluted and re-run. If dilution is not possible, the value must be reported as > highest calibrant concentration and a comment must be made in the LIMS. 12.3. Comments in the LIMS: 12.3.1. A comment must be made into the LIMS under the following circumstances: quality control element failure(s), unacceptable sample integrity/condition, deviation of the SOP, and support equipment failure(s). These circumstances are defined below: 12.3.1.1. Quality Control Element Failure: 12.3.1.1.1. QC element failures includes, but is not limited to the following: blanks/ Quality of Water Check, laboratory control samples, quality control samples, matrix spikes, matrix spike duplicates, duplicates, instrument performance checks, sterility checks, etc.

12.3.1.1.2. QC element failures are batch specific. In the event of a failure, a comment must be added to all samples within the batch.

12.3.1.2. Unacceptable Sample Integrity/Condition as Received: 12.3.1.2.1. Unacceptable sample integrity includes, but is not limited to the following: temperature, pH, container type, leaking sample containers, adequate sample volume, abnormal appearance, etc.

12.3.1.2.2. Sample integrity is sample specific. In the event of unacceptable sample conditions, a comment must be made to the affected sample.

12.3.1.3. Deviation of the SOP: 12.3.1.3.1. Deviation of the SOP includes, but is not limited to the following: upon completion of the test it was determined the SOP was not followed as written and re-analysis of the sample(s) was not possible, the condition of the matrix prevents the analysis to be completed as requested (e.g. pH, sample too thick for by volume analysis, sample too thin for by weight analysis, etc).

12.3.1.3.2. Deviation of the SOP may be batch or sample specific.

12.3.1.3.2.1. If the deviation occurred to an entire batch, a comment must be added to all samples in the batch.

12.3.1.3.2.2. If the deviation occurred to a single sample, a comment must be added to the affected sample.

12.3.1.4. Support Equipment Failure During a Test or Storage: 12.3.1.4.1. Support equipment failure includes, but is not limited to the following: balances, ovens, refrigerators, freezers, incubators, water baths, block digesters, temperature QA_MANUAL_2 Page 25 of 42

measuring devices, thermistors, thermal/pressure sample preparation devices, autoclaves, volumetric dispensing devices and diluter devices.

12.3.1.4.2. Support equipment failures may be batch specific that affect samples and/or standards in more than one batch, or sample specific. In the event of a failure, a comment must be added to all affected samples.

12.4. Corrective Action Form (CAF) 12.4.1. Corrective Action Forms (CAFs) are used to correct systematic problems to minimize or prevent the situation from recurring.

12.4.1.1. CAFs are filled out in the LIMS with information describing the situation that occurred, findings of an investigation, associated samples and appropriate action to correct the problem.

12.4.1.2. Once the CAF has been entered into the LIMS it is automatically sent to the QC Officer for review.

12.4.1.3. The CAF is then used to correct all data from the point at which the situation occurred to when it was resolved.

12.4.1.4. Results are tracked and commented on, citing the CAF number.

12.4.1.5. Clients are notified and new reports are generated.

12.4.1.6. All CAFs are archived in the LIMS for tracking purposes.

12.5. Data Review and Authorization (TNI Standard V1M2: 4.2.8.4p) 12.5.1. After data entry, the data review process may consist of up to 3 types of verification when applicable. These types include multi-level product specification (MLPS), logically inconsistent data (LID), and/or peer review and authorization.

12.5.2. Multi-Level Product Specification (MLPS) 12.5.2.1. The quality of a product (Location/Sampling Point) is often determined by testing a sample and comparing it with a set of limits, known as a product specification. SampleManager (LIMS) is able to check results against a multi-level product specification (MLPS), containing one or more sets of limits for each of the test components. A product level is defined in the MLPS Level table for each set of limits entered. These limits are derived from historical data or permit requirements. The product level controls the way in which the limits are used during result entry and later in result comparison. During result entry, each result entered can be checked against each set of limits in turn. Messages will be displayed on screen, and a VGL program will be called if the result fails any set of limits.

The analyst will perform an appropriate investigation before accepting the result(s). MLPS status (in/out of spec) is stored in the LIMS database.

12.5.3. Logically Inconsistent Data (LID) 12.5.3.1. Logically Inconsistent Data (LID) is defined as results that contradict the logical relationship of the whole being the sum of its parts. One way to spot anomalous data is to see it in the context of related determinations. Such comparisons can spot situations where it appears that the whole is smaller than the sum of its parts. Various types of whole/part relationships can be identified to spot anomalies: different components on the same test, different tests on the same sample, and same tests on different matrices and different samples on the same â&#x20AC;&#x153;streamâ&#x20AC;?. Comparisons against a fixed list of determinations are made at result entry. When a LID is generated the analyst will perform an appropriate investigation and take necessary action to verify and/or correct the result. LID status is stored in the LIMS database.

12.5.4. Peer Review and Authorization (TNI Standard V1M2: 4.2.8.4p) 12.5.4.1. Worksheets, with appropriate QC supplemental documentation, are submitted to the appropriate Section Manager and/or their designee for review.

12.5.4.1.1. If the worksheet and corresponding QC information is complete and accurate, the worksheet will be authorized and completed in the LIMS. 12.5.4.1.2. If the worksheet and/or corresponding QC information is not complete, the worksheet will be submitted back to the analyst for further investigation.

QA_MANUAL_2 Page 26 of 42

13. Laboratory Records, Retention and Retrieval (4740.2097) 13.1. Record Keeping and Retention Time (4740.2097 Subpart A and B) 13.1.1. All information necessary for the historical reconstruction of data shall be maintained by the laboratory (TNI Standard V1M2: 4.13.3). The laboratory retains all information that produced analytical data for all samples, electronic and/or paper form, for a time period of five years.

13.1.1.1. This information is stored in the LIMS and/or Iron Mountain.

13.1.2. The laboratory maintains an up to date signature log of all designated personnel’s signature and initials.

13.1.2.1. All new laboratory staff must sign and initial the signature log prior to any analysis.

13.1.2.2. All staff must immediately inform management when changes in their name occur.

13.1.2.3. All staff are required to maintain consistency with initialing entries in the same manner as the signature log.

13.2. LIMS and Electronic Files (4740.2097 Subpart D – J) 13.2.1. All data and laboratory activities such as sample receipt, sample preparation or data verification is archived in the LIMS and can be retrieved at any time.

13.2.2. Each time an initial result is entered in the LIMS the date, time and analyst entering results is automatically tracked.

13.2.3. An audit trail is used to track any modifications made to a result in the LIMS. 13.2.3.1. The modification is stamped with the date, time and name of analyst performing the modification.

13.2.3.2. The LIMS is configured to retain the initial result even after modifications have been made.

13.3. Paper Records (4740.2097 Subpart D – J) 13.3.1. All paper entries must contain the recorder’s initials including but not limited to: worksheets, labels, and logbook entries (standards, maintenance, calibrations, temperature, volumetric checks, etc.)

13.3.2. All records that are handwritten must be legible and in permanent ink.

13.3.3. When making any corrections to any data, all corrections must be made with one strike through line.

13.3.3.1. This must be done in such a way so the error is still legible.

13.3.3.2. The analyst must then initial the correction(s).

14. Data Integrity Procedures (TNI Standard V1M2: 4.2.8.1) 14.1. These procedures provide assurance that a highly ethical approach to testing is a key component of all laboratory planning, training and implementation of methods.

14.1.1. Data Integrity Training (TNI Standard V1M2: 5.2.7) 14.1.1.1. Shall be provided by the Lab Manager or their designee one time per year.

14.1.1.2. The topics covered in the training session will be documented in writing and provided to all employees. Key topics must include: 14.1.1.2.1. Organizational mission and its relationship to the critical need of honesty and full disclosure in all analytical reporting 14.1.1.2.2. How and when to report data integrity issues 14.1.1.2.3. Record keeping 14.1.1.3. All current employees and new employees are required to take data integrity training once per year.

14.1.1.4. Attendees of the training will sign a Data Integrity Training Signature Attendance Sheet.

The QA Officer or their designee is responsible for creating the sheet.

14.1.1.5. New employees will undergo data integrity training and sign the Data Integrity Training Signature Attendance Sheet as a formal part of new employee orientation.

14.1.1.6. Signature Attendance Sheet(s) and a copy of the training materials will be filed in the QA Office and made available for auditor review.

14.1.2. Periodic monitoring of data integrity 14.1.2.1. The QA Officer or their designee shall look for evidence of inappropriate actions or vulnerabilities related to data integrity as part of their overall internal auditing program.

QA_MANUAL_2 Page 27 of 42

14.1.2.2. Potential issues will be fully investigated.

14.1.2.3. All investigations that result in finding of inappropriate activity shall be documented and shall include any disciplinary actions involved, corrective actions taken, and all appropriate notifications of clients.

14.1.2.4. All documentation of these investigations and actions taken shall be maintained in the QA Office for at least five years.

14.1.3. The Lab Manager, QA Officer, and Section Managers: 14.1.3.1. Shall uphold the spirit and intent of the data integrity procedures.

14.1.3.2. Shall effectively implement the specific requirements of the data integrity procedures.

14.1.3.3. Employees are encouraged to confidentially report suspected unethical behavior that is related to data integrity to the Lab Manager, QA Officer or Section Managers. Such instances will be fully investigated and documented as described in Section 14.1.2.3 and 14.1.2.4 .

15. Complaint Procedures (TNI Standard V1M2: 4.2.8.3n, and 4.8) 15.1. All data review requests should be made to the Analytical Support and Customer Service Section Manager, Dave Fuchs at dave.fuchs@metc.state.mn.us or (651) 602-8115.

15.2. All customer/staff concerns or complaints should be made to the Laboratory Manager, John Hubbling at john.hubbling@metc.state.mn.us or (651) 602-8135.

16. Management Reviews 16.1.

The laboratory manager is scheduled to meet weekly with the QA Officer and QA staff to review issues related to quality systems, and establish plans and timetables to implement program corrections and improvements. These issues include but are not limited to: revisions to the QA manual; feedback from Laboratory managers; results of internal and external assessments (audits, PT sample results, client feedback, etc.) Decisions made at these meetings are recorded.

16.2.

Laboratory Management is scheduled to meet monthly with primary clients to discuss status of current projects and plans for future projects.

16.3.

In addition, on an annual basis, the Laboratory Manager reviews strategic issues and plans, including relevant QA issues, with upper management; this report is reviewed and stored by upper management.

QA_MANUAL_2 Page 28 of 42

Appendix A

Metropolitan Council Working for the Region, Planning for the Future Demonstration of Capability (DOC) for Chemistry Name: ___________________________________________

Scope of Certification LIMS Test Code:_____________________

SOP ID:__________________________________________

Analytes:___________________________

Analytical Technique(s):_____________________________

___________________________________

(e.g. extraction, digestion, Complete SOP)

Initial Demonstration

Re-certification

Certified Trainer: _______________________________ Date of Initial Training Period: ____________________ Standard (Name/Concentration):__________________ DOC Data Source:

PT

Method Standard

Matrix:_______________________________________ Date Demonstration Performed: ___________________ Sample Results

Acceptable Range

Data Acceptable (check one)

1.

YES

NO

2.

YES

NO

3.

YES

NO

4.

YES

NO

Note: Please attach/staple a copy of the worksheet, raw data/benchsheet and computer printout for review by quality control officer. Please attach additional sheet(s) for multi-component analyses.

*Signature of the analyst signifies that they have read, understood and agree to perform the above named analysis using the current version of the standard operating procedure(s) and the current quality assurance manual.

Analyst Name*

Signature

Date

Certified Trainer's Name (Initial DOC Only)

Signature

Date

Lam Sanouvong Quality Control Officer's Name

Signature

Date

The analyst is considered certified for the above named analyte(s), test code and technique, as of the date by the QA Officer. The original form is maintained in the QA officerâ&#x20AC;&#x2122;s files.

QA_MANUAL_2 Page 29 of 42

Appendix B

Metropolitan Council Working for the Region, Planning for the Future Demonstration of Capability (DOC) for Microbiology Name: ___________________________________________

Scope of Certification LIMS Test Code:________________ Analytes:______________________

SOP ID:_________________________________ Analytical Technique(s):_____________________________

______________________________

(e.g. extraction, digestion, Complete SOP)

Initial Demonstration

Re-certification

Certified Trainer: _______________________________ Date of Initial Training Period: ____________________ Standard (Name/Concentration):__________________ DOC Data Source:

PT

Data Comparison

Date Demonstration Performed: ___________________ Sample Results

Acceptable Range

Data Acceptable (check one)

1.

YES

NO

2.

YES

NO

3.

YES

NO

4.

YES

NO

Note: Please attach/staple a copy of the worksheet, raw data/benchsheet and computer printout for review by quality control officer.

Please attach additional sheet(s) for multi-component analyses. *Signature of the analyst signifies that they have read, understood and agree to perform the above named analysis using the current version of the standard operating procedure(s) and the current quality assurance manual.

Analyst Name*

Signature

Date

Certified Trainer's Name (Initial DOC Only)

Signature

Date

Lam Sanouvong Quality Control Officer's Name

Signature

Date

The analyst is considered certified for the above named analyte(s), test code and technique, as of the date by the QA Officer. The original form is maintained in the QA officerâ&#x20AC;&#x2122;s file.

QA_MANUAL_2 Page 30 of 42

APPENDIX C

Metropolitan Council Working for the Region, Planning for the Future

Certified Standard Operating Procedures Certified Parameter

Certified Reference Method

MCES Reference

Alkalinity, as CaCO3 Ammonia, as N Biochemical Oxygen Demand, carbonaceous Biochemical Oxygen Demand, carbonaceous Chemical Oxygen Demand Chloride Chloride Cyanide, Total Hardness Kjeldahl Nitrogen, Total (TKN) Nitrate, as N Phenolics, Total Phosphorus, Total (TP) Residue, Nonfilterable (TSS) Sulfate Metals Mercury E.Coli Bateria E.Coli Bateria Fecal Coliform PCB/Pesticide Purgeable Organic Compounds Semi-Volatile Organic Compounds Acrolein/Acrylonitrile

EPA 310.2 EPA 350.1 Rev. 2.0 SM 5210 B-01 Hach 10360 EPA 410.4 Rev. 2.0 SM 4500-Cl- E-97 EPA 300.0 Rev. 2.1 EPA 335.4 Rev. 1.0 SM 2340 C-97 EPA 351.2 Rev. 2.0 SM 4500 NO3- H-00 EPA 420.1 MNPBMS 014 (365.4) MNPBMS 016 (2540D) EPA 300.0 Rev 2.1 MNPBMS 003 (200.8) MNPBMS 015 (245.7) Coliert Quanti-Tray Coliert-18 Quanti-Tray EPA 600/8-78-017 p.124 EPA 608 Appendix A EPA 624 Appendix A EPA 625 Appendix A EPA 1624B Appendix A

ALK_AA NH3_AA BOD5 BOD5 COD CHLORIDE_AA ANIONS_IC HCN HARD-TITR NUT_AA N-N_AA PHENOL NUT_AA TSSVSS ANIONS_IC MET-ICPMSV HG_CVGF COLI-Q COLI-Q FCOLI_MF PCB-PST-GCV PURG-PP-MSV BNA-MSV ACR-MS

Metropolitan Council Environmental Services 2400 Childs Rd Saint Paul, Minnesota 55106 US EPA Lab Code: MN00025

QA_MANUAL_2 Page 31 of 42

APPENDIX D

QA_MANUAL_2 Page 32 of 42

APPENDIX E

Test Codes by Analytical Section Certified Test Codes Identity BOD5C

FCOLI-MF TSS-GF AG-MSV AL-MSV AS-MSV BA-MSV BE-MSV CD-MSV CO-MSV CR-MSV CU-MSV

Description BOD; 5-day; carbonaceous E Coliform bacteria count; multiple tube fermentation, Quanti-Tray Method Fecal Coliform bacteria count; membrane filtration Suspended solids Silver; ICPMS; by volume Aluminum; ICPMS;by volume Arsenic; ICPMS; by Volume Barium; ICPMS; by volume Beryllium; ICPMS;by volume Cadmium; ICPMS;by volume Cobalt by ICPMS; by volume Chromium; ICPMS;; by volume Copper; ICPMS; by volume

HG-CVGF7V

Mercury;Epa 245.7;w/o 1669;cold vapor; single gold amalgam; florescence; PSA Automated; >1.0 ng/l (Bromine Digestion) by volume

ECOLI-MPNT

Group ID BIO/RESIDUE

Analysis Type BOD5

BIO/RESIDUE

ECOLI-MPNT

BIO/RESIDUE BIO/RESIDUE METALS METALS METALS METALS METALS METALS METALS METALS METALS

FCOLI-MF TSS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS

METALS

HG-CVGF7

METALS

HG-CVGF7

METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS WET CHEM

ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ALK-AV

MET-MSV MN-MSV MO-MSV NI-MSV PB-MSV SB-MSV SE-MSV TL-MSV V-MSV ZN-MSV ALK-AV

Mercury;Epa 245.7;with 1669;cold vapor; single gold amalgam; florescence; PSA Automated; >1.0 ng/l (Bromine Digestion) by volume Metals: Cu; NI; Pb; Zn; Cd; Cr; by ICPMS;by volume Manganese; ICPMS;by volume Molybdenum; ICPMS;by volume Nickel; ICPMS;by volume Lead; ICPMS;by volume Antimony; ICPMS;by volume Selenium; ICPMS; by volume Thallium; ICPMS;by volume Vanadium; ICPMS;by volume Zinc; ICPMS;by volume Alkalinity; autoanalysis by volume

ANION-ICV CL-AV

Anions by Ion Chromatography;by volume; Nitrate N; Nitrite N; Sulfate; Chloride; Ortho P; Fluoride; Bromide Chloride ion; autoanalysis by volume

WET CHEM WET CHEM

ANION-IC CL-AV

CL-ICV

Chloride by Ion Chromatography;by volume;

WET CHEM

CL-IC

COD-A2

Chemical Oxygen Demand; with Silver Slufate; sealed ampule; spectrophotometric

WET CHEM

COD-A

HG-CVGF97V

QA_MANUAL_2 Page 33 of 42

COD-AW2

Chemical Oxygen Demand; by weight; with silver sulfate; sealed ampule; spectrophotometric

WET CHEM

COD-A

WET CHEM

HARD

WET CHEM

HCN-A2

WET CHEM

N_N-A

WET CHEM

NH3-A

WET CHEM

NUTS-A

WET CHEM

NUTS-AR

WET CHEM WET CHEM

NUTS-A NUTS-A

SO4-ICV

Hardness; high level:>5mg/l; EDTA titration Cyanide;semi micro digest;auto color;vol: acid reflux; barbituric acid colorimetry Nitrate/Nitrite-N;28 day hold time;autoanalysis by volume Ammonia Nitrogen; phenate; autoanalysis; by volume Kjeldahl Nitrogen and Phosphorus; total; autoanalysis by volume RUSH' Kjeldahl Nitrogen and Phosphorus; total; autoanalysis by volume Kjeldahl Nitrogen and Phosphorus; total; autoanalysis by weight Phosphorus; total; autoanalysis by volume Sulfate Anion by Ion Chromatography;by volume;

WET CHEM

SO4-IC

TKN-AV

Kjeldahl Nitrogen; total; autoanalysis by volume

WET CHEM

NUTS-A

ORGANICS

ACR-MS

ORGANICS ORGANICS

PCB-GC PNL-4A

ORGANICS

PPBNA-MS

PPP-MSV

PP Base Neut.+Acid Extract. by MS by Volume Purgeable Prior. Poll. by MS by vol; alph and aromat

ORGANICS

PPP-MS

PPPST-GCV

Pesticides by GC by vol from PP list; w/ PCB

ORGANICS

PPPST-GC

HARD-HL HCN-A2V N_N-AV NH3N-AV NUT-AV NUT-AVR NUT-AW P-AV

ACR-MSV PCB-GCV PNL-4A PPBNA-MSV

Acrolein and acrylonitrile by MS; by vol.

Ambient purge with isotope dilution epa 1624 Polychlorinated Biphenyls by Arochlor by GC by volume Phenolics; total; 4-AAP colorimetric

Certified and Non-Certified Test Codes Identity

Description

Group ID

Analysis Type

BOD5 BOD5C

BOD; 5-day; carbonaceous and nitrogeneous BOD; 5-day; carbonaceous

BIO/RESIDUE BIO/RESIDUE

BOD5 BOD5

BODTOX

BOD Toxicity - Evaluate potential toxicity of material as indicated by increasing BOD with increasing BOD dilutions

BIO/RESIDUE

BODTOX

BODUC70-DN

BOD; ultimate; carbonaceous and nitrogeneous seperate; by Delta N method. Glass bead method in 2 Liter BOD bottle.

BIO/RESIDUE

BODUC70-DN

BIO/RESIDUE

CL2

BIO/RESIDUE

CL2-DPD-C

CL2-BIA CL2-DPD-C

Chlorine residual; amperometric; backtitration with I2; measures strong oxidants reported as CL2.

CHLORINE RESIDUAL;DPD COLORIMETRIC

QA_MANUAL_2 Page 34 of 42

CL2-FTS

Chlorine residual; forward titration; with thio; starch endpoint; measures strong oxidants reported as CL2

BIO/RESIDUE

CL2-FTS

CLA-BMTRCS

Chlorophylls on surfaces (sonify): trichrom a; b and c; pheophytin corrected and viability indicators;extraction by sonification in 90% acetone.

BIO/RESIDUE

CLA

CLA-TR-CS

Chlorophylls (sonify): trichrom a; b and c; mono chrom- pheophytin corrected and viability indicators;extraction by sonification in 90% acetone.

BIO/RESIDUE

CLA

COLI-MPNT

Total Coliform bacteria and E Coliform bacteria count; multiple tube fermentation, Quanti-Tray Method

BIO/RESIDUE

ECOLI-MPNT

BIO/RESIDUE BIO/RESIDUE BIO/RESIDUE

COLI-PA COND DOX

BIO/RESIDUE

ECOLI-MPNT

BIO/RESIDUE

FCOLI-MF

BIO/RESIDUE

FCOLI-MPN

BIO/RESIDUE

FCOLI-MPN

BIO/RESIDUE

FCOLI-MPN

FCOLI-MPNB

Total Colifrom bacteria; presence/absence; Chromgenic Substrate Technique; Colitert indicator ONPG-MUG; (Ortho-nitrophenyl-BD-galactopyranoside - 4-methylumbelliferyl-Bd-glucoronide) Conductivity Dissolved oxygen; winkler titration E Coliform bacteria count; multiple tube fermentation, Quanti-Tray Method Fecal Coliform bacteria count; membrane filtration Fecal Coliform bacteria count; multiple tube fermentation Fecal Coliform bacteria count; multiple tube fermentation; Class A detemination Fecal Coliform bacteria count; multiple tube fermentation; Class B Determination

HETEROPCNT MICROEXAM

Heterotrophic plate count (bacteria); pour plate Microscopic examination and description

BIO/RESIDUE BIO/RESIDUE

HETER0PCNT MICROEXAM

ML_EXAM

BIO/RESIDUE

ML_EXAM

BIO/RESIDUE

ML_TAX

BIO/RESIDUE

ECOLI-MPNT

BIO/RESIDUE

TURB

BIO/RESIDUE BIO/RESIDUE BIO/RESIDUE BIO/RESIDUE

BM_SOLIDS BTU DENSITY GRIT_SLDG

POLY_EXSOL TDS-180

Microscopic exam. and desc. of mixed liquor Microscopic exam and Taxonomic identification of filaments in Mix Liquor Total Coliform bacteria count; multiple tube fermentation, Quanti-Tray Method Turbidity by nephelometry; Nephelometric Turbidity Ratio Units; Hach 2100N Meter; Biomass solids; hydrated volatile solids plus sediment free dry weight British Thermal Units; O2 bomb Density Grit in sludge; Zimpro warrenty procedure Polymer solids by acetone extraction technique at 70 deg C Total Dissolved Solids; dried at 180 deg C

BIO/RESIDUE BIO/RESIDUE

POLYSOLIDS TDS-180

TOXORG-WT

Toxicity testing Organism Weight; to 0.00001gm (max=30gm) using analytical balance

BIO/RESIDUE

WEIGHT

COLI-PA COND DOX-W ECOLI-MPNT FCOLI-MF FCOLI-MPN FCOLI-MPNA

ML_TAX TCOLI-MPNT TRB-NTRUN2 BM_SOLIDS BTU-O2 DENSITY-WV GRIT_SLDG

QA_MANUAL_2 Page 35 of 42

TS_ASH-VOL TSS-GF TSSVSS-GF TS-VOL

Fixed solids; volumetric basis; 550 deg C Suspended solids Suspended and volatile suspended solids Total solids; volumetric basis; 103 deg C

BIO/RESIDUE BIO/RESIDUE BIO/RESIDUE BIO/RESIDUE

TS_ASH TSS VSS TS

TSVS-VOL

Total and volatile solids; volumetric basis (mg/l)

BIO/RESIDUE

TVS

TSVS-WT

Total and volatile solids; weight basis(%); 550C

BIO/RESIDUE

TVS

TSVS-WTC TS-WT

Total and volatile solids; weight basis(%); 550C Dry to constant weight; Method 2540G SM 18th edition Total solids weight basis (%)

BIO/RESIDUE BIO/RESIDUE

TVS TS

BIO/RESIDUE

TS

BIO/RESIDUE METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS

WEIGHT ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS

METALS METALS METALS METALS METALS METALS METALS

CR6-ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS

CR6-MSV CR-MSV CR-MSW CU-MSV CU-MSW FE-MSV FE-MSW

Total solids weight basis (%); Dry to constant weight; Method 2540G SM 18th edition Weight to 0.01gm (min=1gm) using top loader balance Silver; ICPMS; by volume Silver; ICPMS ; by weight Aluminum; ICPMS;by volume Aluminum; ICPMS; by weight Arsenic; ICPMS; by Volume Arsenic; ICPMS; by Weight Barium; ICPMS; by volume Barium; ICPMS by weight Beryllium; ICPMS;by volume Beryllium; ICPMS; by weight Boron by ICPMS;by volume Boron by ICPMS by weight Calcium; ICPMS; by volume Calcium; ICPMS; by weight Cadmium; ICPMS;by volume Cadmium; ICPMS; by weight Cobalt by ICPMS; by volume Cobalt by ICPMS; by weight Hex. chromium; filtered. precip/ICPMS method.

USEPA Method 218.5 Chromium; ICPMS;; by volume Chromium; ICPMS; by weight Copper; ICPMS; by volume Copper; ICPMS; by weight Iron; ICPMS; by volume Iron; ICPMS; by weight

HG-CVAW

Mercury; cold vapor AAS; PSA Automated; >1.0 ug/kg(Permangate Digestion) by weight

METALS

HG-CVA

HG-CVFV

Mercury; cold vapor; fluorescence; PSA Automated; >1.0 ng/l (Bromine Digestion) by volume

METALS

HG-CVF

HG-CVFW

Mercury; cold vapor; fluorescence; PSA Automated; >1.0 ng/l (Bromine Digestion) by weight

METALS

HG-CVF

TS-WTC WEIGHT AG-MSV AG-MSW AL-MSV AL-MSW AS-MSV AS-MSW BA-MSV BA-MSW BE-MSV BE-MSW B-MSV B-MSW CA-MSV CA-MSW CD-MSV CD-MSW CO-MSV CO-MSW

QA_MANUAL_2 Page 36 of 42

HG-CVGF7V

Mercury;Epa 245.7;w/o 1669;cold vapor; single gold amalgam; florescence; PSA Automated; >1.0 ng/l (Bromine Digestion) by volume

METALS

HG-CVGF7

HG-CVGF97V

Mercury;Epa 245.7;with 1669;cold vapor; single gold amalgam; florescence; PSA Automated; >1.0 ng/l (Bromine Digestion) by volume

METALS

HG-CVGF7

METALS METALS METALS

HG-CVA ICPMS ICPMS

METALS

ICPMS

METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS METALS WET CHEM

ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ICPMS ALK-AV

MET-MSW MG-MSV MG-MSW MN-MSV MN-MSW MO-MSV MO-MSW NA-MSV NA-MSW NI-MSV NI-MSW PB-MSV PB-MSW SB-MSV SB-MSW SE-MSV SE-MSW SN-MSV SN-MSW TI-MSV TI-MSW TL-MSV TL-MSW V-MSV V-MSW ZN-MSV ZN-MSW ALK-AV

Mercury; Spke; cold vapor AAS; PSA Automated; >1.0 ug/kg(Permangate Digestion) by weight Potassium; ICPMS;by volume Potassium; ICPMS; by weight Metals: Cu; NI; Pb; Zn; Cd; Cr; by ICPMS;by volume Metals: Cu; NI; Pb; Zn; Cd; Cr; by ICPMS; by weight Magnesium; ICPMS;by volume Magnesium; ICPMS; by weight Manganese; ICPMS;by volume Manganese; ICPMS; by weight Molybdenum; ICPMS;by volume Molybdenum; ICPMS; by weight Sodium; ICPMS;by volume Sodium; ICPMS; by weight Nickel; ICPMS;by volume Nickel; ICPMS; by weight Lead; ICPMS;by volume Lead; ICPMS; by weight Antimony; ICPMS;by volume Antimony; ICPMS; by weight Selenium; ICPMS; by volume Selenium; ICPMS;by weight Tin; ICPMS;by volume Tin; ICPMS; by weight Titanium; ICPMS; by volume Titanium;ICPMS; by weight Thallium; ICPMS;by volume Thallium; ICPMS; by weight Vanadium; ICPMS;by volume Vanadium;ICPMS; by weight Zinc; ICPMS;by volume Zinc; ICPMS; by weight Alkalinity; autoanalysis by volume

ANION-ICV

Anions by Ion Chromatography;by volume; Nitrate N; Nitrite N; Sulfate; Chloride; Ortho P; Fluoride; Bromide

WET CHEM

ANION-IC

ANION-ICW CL-AV

Anions by Ion Chromatography;by weight; Nitrate N; Nitrite N; Sulfate; Chloride; Ortho P Chloride ion; autoanalysis by volume

WET CHEM WET CHEM

ANION-IC CL-AV

CL-ICV

Chloride by Ion Chromatography;by volume;

WET CHEM

CL-IC

HG-SPKCVA K-MSV K-MSW MET-MSV

QA_MANUAL_2 Page 37 of 42

COD-A2

Chemical Oxygen Demand; with Silver Slufate; sealed ampule; spectrophotometric

WET CHEM

COD-A

COD-AW2

Chemical Oxygen Demand; by weight; with silver sulfate; sealed ampule; spectrophotometric

WET CHEM

COD-A

WET CHEM

HARD

WET CHEM

HCN-A2

WET CHEM

HCN-A2

WET CHEM

HCNCL2-A2

WET CHEM

N_N-A

WET CHEM

N_N-A

WET CHEM

N_N-A

WET CHEM

NH3-A

WET CHEM

NH3-A

WET CHEM

NUTS-A

WET CHEM

NUTS-A

WET CHEM

NUTS-AP

WET CHEM

NUTS-A

WET CHEM

NUTS-AR

WET CHEM

NUTS-A

WET CHEM

OIL-SOX

WET CHEM

OIL-SOX

WET CHEM

OIL-SOX

WET CHEM

ORTHO_P

HARD-HL HCN-A2V HCN-A2W

HCNCL2-A2V N_N-AHV N_N-AV N_N-AW NH3N-AV NH3N-AW NUT-AHLV NUT-ALV

NUT-AP NUT-AV NUT-AVR NUT-AW OIL-SOX OIL-SOX2 OIL-SOXW2 ORTHO_P

ORTHO_PH P-AHLV P-ALV

Hardness; high level:>5mg/l; EDTA titration Cyanide;semi micro digest;auto color;vol: acid reflux; barbituric acid colorimetry Cyanide;semi micro digest;auto color; wgt: acid reflux; barbituric acid colorimetry Cyanide amenable to Cl2;man dig;auto color;vol: acid reflux; barbituric acid colorimetry (diff. between chlorinated samp. and straight samp.)

Nitrate/Nitrite-N;extended holding time;autoanalysis by volume Nitrate/Nitrite-N;28 day hold time;autoanalysis by volume Nitrate and Nitrite Nitrogen; autoanalysis by weight Ammonia Nitrogen; phenate; autoanalysis; by volume Ammonia Nitrogen; phenate; autoanalysis; by weight Phos + Kjel; Low Level; Extended Holding time; Auto Analysis PHOS (TOT) AND KJEL. NIT; LOW LEVEL AUTO ANAL Kjeldahl Nitrogen and Phosphorus; particulate matter; autoanalysis; reporting units corrected to mg/l.

Kjeldahl Nitrogen and Phosphorus; total; autoanalysis by volume RUSH' Kjeldahl Nitrogen and Phosphorus; total; autoanalysis by volume Kjeldahl Nitrogen and Phosphorus; total; autoanalysis by weight Oil and Grease; freon soxhlet extraction; gravametric Oil and Grease; Hexane soxhlet extraction; gravametric Oil and Grease; hexane soxhlet extraction; gravametric; by weight Phosphorus; ortho; manual phospho-molybdate spectrophotometric

Phosphorus; ortho; manual phospho-molybdate spectrophotometric; non-std;extended holding time;frozen samples WET CHEM Phosporus (Tot); Low Level; Extended Holding time-samples frozen; Auto Anal WET CHEM PHOSPHORUS (TOT);LOW LEVEL AUTO ANAL WET CHEM QA_MANUAL_2 Page 38 of 42

ORTHO_P NUTS-A NUTS-A

SO4-ICV

Phosphorus; particulate matter; autoanalysis; reporting units corrected to mg/l.

Phosphorus; total; autoanalysis by volume Phosphorus; total; autoanalysis by weight Silica; molybdate reactive; Auto Analysis.

Sulfate Anion by Ion Chromatography;by volume;

TKN-AP

Kjeldahl Nitrogen; particulate matter; autoanalysis; reporting units corrected to mg/l.

WET CHEM

NUTS-AP

TKN-AV

Kjeldahl Nitrogen; total; autoanalysis by volume

WET CHEM

NUTS-A

TKN-AW

Kjeldahl Nitrogen; total; autoanalysis by weight Total Organic Carbon; high temp combustion module; NDIR detection Total Organic Carbon; wet oxidation; auto sampler;settled sample; NDIR detection

WET CHEM

NUTS-A

WET CHEM

TOC-HTC

WET CHEM

TOC-WO

WET CHEM

TOC-WO2

ORGANICS

ABS-UV

ORGANICS

ACR-MS

ORGANICS

BTEX-MS

ORGANICS ORGANICS

PPBN-MS DYE-FL

ORGANICS

MS-ID

P-AP P-AV P-AW SILICA-AV

TOC-HTC TOC-WO

TOC-WO2 ABS-UV

Total Organic Carbon; wet oxidation; Manual injection - mixed sample; NDIR detection ABSORBANCE/TRANSMITTANCE (UV/VIS) AT SPECIFIED WAVELENGTH

WET CHEM WET CHEM WET CHEM WET CHEM

NUTS-AP NUTS-A NUTS-A SILICA

WET CHEM

SO4-IC

MS-ID

Acrolein and acrylonitrile by MS; by vol.

Ambient purge with isotope dilution epa 1624 Benzene; Toluene; Ethly Benzene; and Xylenes

by MS by vol(BTEX) Base neutrals by MS from Centralized Waste Treatment list; by vol Dyes; quantitation by fluorescence MS ID or verification (P/A) of suspected sample components.

MS-SCAN

Mass spectral scan of unknown mix with tentative ID of selected components and 'area count quantification' (ie w/o standards)

ORGANICS

MS-SCAN

MS-SCAN2

Mass spectral scan with confirmation and quantitation of components in unknown mixture in samples thru use of pure standards.

ORGANICS

MS-SCAN2

ORGANICS

PCB-AGCW

ORGANICS

PCB-GC

ORGANICS ORGANICS

PCB-GC PNL-4A

ORGANICS

PPBNA-MS

ACR-MSV BTEX-MSV CWTBN-MSV DYE-FL

PCB-GCW PNL-4A

PCB's; by Arochlor by GC by weight Accelerated Solvent Extraction with GPC and Micro/Macro SPE (Solid Phase Extraction) cleanup Techniques Polychlorinated Biphenyls by Arochlor by GC by volume Polychlorinated Biphenyls by Arochlor by GC by weight Phenolics; total; 4-AAP colorimetric

PPBNA-MSV

PP Base Neut.+Acid Extract. by MS by Volume

PCB-AGCW PCB-GCV

QA_MANUAL_2 Page 39 of 42

PPBNA-MSW

PP Base Neut.+Acid Extract. by MS by weight

ORGANICS

PPBNA-MS

PPBNA-SPKM

ORGANICS

PPBNA-MS

PPBN-MSV

PP Base Neut.+Acid Extract SPIKE by MS Base neutrals by MS from Priority Pollutant list; by vol

ORGANICS

PPBN-MS

PPPAR-MSV

Purgeable aromatic Prior Poll by MS by vol

ORGANICS

PPPAR-MS

PPPAR-MSW

Purgeable aromatic Prior Poll by MS by weight Purgeable Prior. Poll. by MS by vol; alph and aromat Purgeable Prior. Poll. by MS by weight alph and aromat Purgeable Prior. Poll. SPIKE by MS; alph and aromat

ORGANICS

PPPAR-MS

ORGANICS

PPP-MS

ORGANICS

PPP-MS

ORGANICS

PPP-MS

Pesticides by GC by vol from PP list; w/ PCB Pesticides by GC by weight from Prior Poll list; w/ PCB

ORGANICS

PPPST-GC

ORGANICS

PPPST-GC

PH

pH by electrochemical pH probe

PRECIP

Metro Plant Daily Precipitation

TEMP

INFLUENT TEMPERATURE

ORGANICS ORGANICS ORGANICS ANALYTICAL SUPPORT ANALYTICAL SUPPORT ANALYTICAL SUPPORT ANALYTICAL SUPPORT

PPPST-GC VA-ABT VA-GC

MINMAXPH

Pesticides by GC SPIKE from PP list; w/ PCB Volatile acids (total) by acid/base titration Volatile acids by GC by vol FIELD DATA: Min and Max pH values read from Primary Influent pH graphs

PPP-MSV PPP-MSW PPP-SPKM PPPST-GCV PPPST-GCW PPPST-SPKG VA-ABT VA-GCV

QA_MANUAL_2 Page 40 of 42

FIELD_DATA PH FIELD_DATA FIELD_DATA

APPENDIX F 4740.2099 Initial Demonstration of Analyst Certification Database Date Last Updated: Updated By:

Date

Name/Analyst

Employee

Training

Status

Completed

(P= Past)

Test Code

SOP

Analytical

Supervisor/Trainer

Date Recertification

Date Recert.

Identification #

Technique

Name

Required

Completed

QA_MANUAL_1 Page 41 of 42

APPENDIX G

Laboratory Services Sample Acceptance Policy Minnesota Department of Healthâ&#x20AC;&#x2122;s (MDH) Laboratory Certification rules require that Laboratories establish a Sample Acceptance Policy (SAP) and make it available to sample collection personnel. This policy should clearly outline the circumstances under which samples will be accepted or rejected by the laboratory. Due to the MPCA regulatory monitoring requirements, rather than rejecting samples, Laboratory Services staff will put procedures into place to check for sample irregularities. Such irregularities will be documented and communicated to sample collection personnel. Laboratory staff is always responsible for preparing Agency compliance reports. Documented sample irregularities will be incorporated into these report generation processes.

All samples subject to Laboratory Certification protocols must be collected and preserved according to Code of Federal Regulations 40 Part 136 table II. Upon receipt in the laboratory, samples will be handled according to the following Laboratory Services SAP Criteria: 1. Sample Missing Sheets (SMS) SMSs are prepared for each sample set. A SMS indicates what samples were delivered, sampling interval and delivery date.

2. Sample Condition.

Sample bottles received by laboratory staff are inspected for the following; a) Use of appropriate, permanently marked Laboratory Services provided containers; b) Integrity of Sample Containers (sealed and check for leakage); and c) Adequate Sample Volume. Any deviations from acceptable condition are noted on the SMS. Any deviations are reported to the sample collection personnel to be addressed. Any deviations that might impact analysis or reporting of results for affected samples are addressed according to fixed agency protocols, or are discussed with in the Agency and a specific response established.

3. Proper thermal preservation All samples requiring thermal preservation should arrive to the laboratory in coolers containing ice, indicating at a minimum, that the cooling process has begun. Deviations of this protocol will be noted on the appropriate SMS. Absence of ice in the coolers will be considered a deviation to this protocol and will be reported back to sample collection personnel to be addressed.

4. Proper Acid/Base Chemical Preservation The pH of the preserved sample will be checked to validate complete preservation. If preservation is not complete, additional preservation chemical will be added and the deviation noted on the SMS and corrective action will be initiated.

QA_MANUAL_1 Page 42 of 42