Antibody Reformatting and Production
Our Capabilities for Various Antibody Reformatting Antibody variable regions of heavy chain (VH) and light chain (VL) from a specific antibody can be reformatted into a diversity of fragments including Fab, F(ab)’2, scFv, VHH, minibodies, and also BsAbs combined with a second specificity. Various antibody formats are optimized for tumor or tissue penetration, or expression in production systems. Here are our current available antibody reformatting choices.
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Fab
Fab can benefit from the absence of FcR receptor mediated effector functions;
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Can be easily expressed in non-mammalian expression systems.
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Retain the bivalent binding of an IgG;
F(ab)’2
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VHH
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Fab has good characterized penetration properties into tissues and faster clearance from circulation;
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BsAbs
Fab is monovalent and is approximately only a third of the size of a whole IgG;
Without Fc portion which mediates the effector functions via it’s interaction with FcR receptors
Engineered by linking the variable domains of heavy chain and light chain into a single molecule;
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scFv is only approximately a fifth of the size of a whole IgG;
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Routinely used for phage display;
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Linked to a wide range of fusion proteins for specific targeting.
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Consist of two different binding domains which can target separate antigens;
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Critically useful when directing an effector cell to a target cell more effectively;
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Can be made in various formats;
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Can be designed to customize your requirements as per specific application.
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