CAR-T Platform Offers TCR Gene Transfer Technologies for the Development of the Immuotherapy Tags: gene engineering, gene transfer technology, TCR gene transfer technologies only make great contribution to the immunotherapy, however, with the development of gene engineering, gene therapy and the production of genetically modified organisms, the gene transfer technology has become an important research point in medical field. The so-called gene transfer technology is to introduce the exogenous gene (genomic DNA, cloned gene, oligonucleotides etc.) into receptor organisms or cells through specific methods, and detect the expressed results in transformed cells. This technique used in the application of microorganisms (bacteria, yeast, etc.) and plant cells is called transformation, while used in animal cells, it is referred to as transfection. According to the foreign gene transfer using different media, it can be roughly divided into physical, chemical and biological methods. All of the above methods are the powerful tools for the scientific and clinical researches. We should never ignore their effects. Specifically, in the gene therapy, viral vectors are often used for the TCR gene transfer. Lentiviral vectors and retroviral vectors are two most important examples: Lentiviral vectors Lentivirus including primate lentiviruses, such as human immunodeficiency virus (HIV), simian immunodeficiency virus (SIV) and non primate lentiviruses(feline immunodeficiency virus (FIV), equine infectious anemia virus (EIAV), bovine immunodeficiency virus (BIV) and Weiss - Mehdi virus (VMV)). At present, HIV-1, HIV-2, SIV, FIV and EIAV are widely used as vectors for gene therapy, and HIV-1 is the most popular among them. In recent years, a regulated lentiviral vector is developing, and the vector system will insert the induced gene transfer system into lentiviral vector when it retains the self inactivating characteristics, controlling the gene expression artificially. At present, the use of tetracycline induced exogenous gene expression can be used in the induction system. Retroviral vectors Retroviral mediated gene technology is the most effective way to introduce foreign gene into cells at present. This system includes two parts: the recombinant retroviral vector and the packaging cell. LNL6 is modified by the Moloney murine leukemia virus (MO-MLV). About the author: CAR-T is based on Creative Biolabs to develop and produce medicines in TCR and CAR cell immune therapy. In order to meet various needs from customers, we will design and offer appropriate strategies for different host cells. Source: http://marketersmedia.com/car-t-platform-offers-tcr-gene-transfer-technologies-for-the-
development-of-the-immuotherapy/148018