Microorganisms in the school by Joan Mercader

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BACTERIA IN DIFFERENT DAYTIMES OBJECTIVE To investigate if the daytime influences on the number of bacteria in classrooms. HYPOTESIS At 5 o’clock there will be more microorganisms than other times. EXPERIMENTAL DESIGN ·

MATERIAL

3 Petri dishes Ruler Permanent maker Oven Microscope ·

METHOD

1. First of all we wrote the time of the day on the petri dish with the permanent maker. 2. Then we put one petri dish on a cupboard at 10:50. We closed at 11:05. 3. After we put another petri dish on the same cupboard at 1:15. We closed at 1:30. 4. Then we put the last petri dish on the same cupboard at 4:45. We closed at 5:00. 5. Then we put them in the oven for two weeks, in order to let bacteria grow. 6. Two weeks later we observed the petri dishes with the microscope and we measured the diameter and the number of colonies of each petri dish. RESULTS Chart

Morning (10:50 - 11:05) Lunchtime (1:15 - 1:30) Afternoon (4:45 - 5:00)

Number colonies 27 30 18

of Diameter colonies (mm) 5 5,2 6

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Graphs

Bacteria depending of the time of the day 30 25 20 15 10 5 0

Number of colonies

Morning (10:50 11:05)

Lunchtime Afternoon (1:15 - 1:30) (4:45 - 5:00)

Diameter of colonies (mm)

Time of the day

Pictures

Morning (10:50 - 11:05)

Lunchtime (1:15 - 1:30)

Afternoon (4:45 - 5:00)

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CONCLUSIONS There is more number of colonies at the lunchtime (30 colonies). The less number of colonies is in the afternoon (18). On the morning there are 27. So, we can make the conclusion that the daytime with more bacteria is lunchtime. The diameter of colonies is bigger on the afternoon (6 mm). The smallest diameter of the colonies is in the morning (5 mm). In the lunchtime the diameter of colonies is about 5,2 mm. As we can observe, there is less differences between the diameters of colonies in different daytimes than in the number of colonies.

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WHAT PART OF THE CLASS IS MORE CONTAMINATED BY BACTERIA? OBJECTIVE: To discover the most contaminated place by bacteria in our classroom. HYPOTESIS: We think it may be the lock of the door. MATERIALS: · Petri dishes x4 · Lock of the door x1 · Table x1 · Blackboard x1 · Chair x1 FIXED VARIABLES: · The time · The temperature · The air · The type of the petri dishes · The type room · The people of the room EXPERIMENT DESIGN: We put the petri dishes in each location, at the same temperature and in the same time. After that we evaluate what happens. Then we have the results. And we can do the conclusions.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Roger Vernedas Ignasi Pratdesaba Pere Vinyeta Brahim Katar Artur Roquer

We put the petri dishes ten minutes on the table at twenty degrees.

We put the petri dishes on the lock(handle) of the door ten minutes and at twenty degrees.

We put the petri dishes on the chair ten minutes and at twenty degrees.

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We put the petri dishes on the blackboard ten minutes and at twenty degrees.

CONCLUSIONS: - The most contaminated place is the lock of the door, because is the place that the persons touch more. - The place is less contaminated is the blackboard, because is the place that the persons touch less. - The conclusion agree with the hypothesis

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Microorganisms on the objects in 2nd ESO B 1. OBJECTIVE: Our objective is to investigate witch quotidian object are the most contaminated with bacteria.

2. HYPOTHESIS: Our hypothesis is that there are more bacteria on the door knock because many people touch it every day.

3. EXPERIMENT DESIGN: 3.1 Material: Petri dishes, permanent maker, calculator, mobile phone, microscope, rule, a door knob, a switch, a computer cabinet and a box. 3.2 Method: First of all we wrote on the petri dishes de location they went. Then we put each petri dish in the each place for 15 minutes. After 15 minutes we took the petri dishes and we put them in the oven for two weeks at 37ÂşC, to let bacteria reproduce. Finally we took out and counted the number of colonies and their diameter.

4. RESULTS: 4.1 Chart: 2n ESO B

Number of colonies

Diameter of colonies

Number of species

Door knob

6.21

Computer box

6.6

Computerâ&#x20AC;&#x2122;s cabinet

8.5

Switch

5.9

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4.2 Graphs:

4.3 Photos:

Door Knob

Switch

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Computer’s cabinet

Computer’s box

CONCLUSIONS: ·

The door knob is the most contaminated place followed by the switch.

The door knob and the switch have a lot of more contamination colonies (28 and 24) than the computer’s cabinet and the the PC box (6 and 5).

Although the diameter of the colonies is very similar: ü Door knob : 6,21 cm. ü Switch: 5,9 cm. ü PC’s cabinet: 8,5 cm. ü PC box : 6,6 cm.

As the number of colonies is higher in the door knob and in the switch, there also are more species: ü Door knob: 10 species ü Switch: 8 species ü PC’s cabinet: 6 species ü Pc’s box: 4 species

We can make some predictions: o If we did the same experiment with coins , we probably see that the contamination would be very high, as they are objects that people touches a lot.

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Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Oriol Ferrer, Elagi Josep, Bernat Vilarrasa 2n ESO B Ciències en Anglès

BACTERIA’S LIFE TEMPERATURE

QUESTION: Does the temperature affect on the growth of the microorganisms? Do microorganisms live in high temperature places or in low temperature places?

OBJECTIVE: To discover at what temperatures microorganisms live.

HYPOTHESIS: -We think that microorganisms live in warm places.

EXPERIMENTAL DESIGN: MATERIAL: -4 petri dishes -1 oven -1 chronometer -ruler

METHOD: -We put 4 petri dishes in different places around the classroom: ¨ -Location 1: Near the radiator. (30ºC approx.) ¨ -Location 2: Exposed at the sun. (20ºC approx.) ¨ -Location 3: On the teacher’s table. (16ºC approx.)

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¨ -Location 4: Near the window after open it. (8ºC approx.)

-Ten minutes later we closed it and we put the petri dishes in an oven. -Two weeks later we count the colonies and the diameter of each colony. -We made the average and we knew at what temperature bacteria live.

RESULTS: CHARTS:

LOCATIONS

COLONIES

DIAMETER (average)

SPECIES

RADIATOR (30ºC)

5,4 mm

SUN (20ºC)

5,3 mm

TEACHER’S TABLE (16ºC)

7,25 mm

NEAR THE WINDOW (8ºC)

12,2 mm

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GRAPH:

25 20 15 10 5 0

23 17

30ºC

5 1

30ºC

PHOTOS:

NUMBER OF COLONIES

20ºC

16ºC

8ºC

DIAMETER (AVERAGE IN mm) SPIECIES

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20ºC

16ºC

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8ºC

CONCLUSIONS:

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-The results of the experiment we the same of our hypothesis, because we thought they live at warm places, and it’s true, they live at <20ºC>. -We think that in low temperatures bacteria grow more because they reproduce better in high temperatures. -Depending on the temperature there grow different species of bacteria. They grow more at middle temperatures. -Bacteria won’t grow a lot at 0º temperatures. They can also live at 35º temperatures.

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Dennys Perez Danelle Pelayo Anda Iona Ali Amhare

Microorganisms in the Human Body: do boys have more bacteria than girls? 1) · ·

Objectives To investigate if boys have more bacteria than girls. To discover which parts of the body contains more bacteria.

2) · · 3) · · · · · · ·

Question Which part of the body contains more bacteria? Do boys have more bacteria than girls? Hypothesis The hands contain more bacteria, because we can touch many things. The boys sweat more than girls because, boys always play games. Bacteria can spread all over our body, and it can cause disease. Boys get dirty when they play. The feet can sweat with heat of the climate. Sometimes feet touch the ground, and the ground is full of bacteria. Some poor countries have children without slippers or shoes to wear and their feet can contain bacteria and can cause disease.

4) Experimental Design 4.1) Materials We need: · ·

Two Petri dishes each (2 for girls and 2 for boys) Oven

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Dennys Perez Danelle Pelayo Anda Iona Ali Amhare Girls:

Boys:

4.1) Method I. II. III. IV.

First, we put a foot or a hand on a petri dish, and wait for 15 minutes. After 15 minutes, we put the petri dishes inside the oven, and wait two weeks. Then we counted the number of colonies and the diameter of each colony in each petri dish. Then we wrote the results in a chart.

5) Results

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Dennys Perez Danelle Pelayo Anda Iona Ali Amhare

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com CLIL 2n ESO B

Dennys Perez Danelle Pelayo Anda Iona Ali Amhare

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com CLIL 2n ESO B

Dennys Perez Danelle Pelayo Anda Iona Ali Amhare

Conclusion In this investigation, weâ&#x20AC;&#x2122;ve checked that girls have more bacteria than boys.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Emma Subirachs Marc Cassany Gil FarrĂŠs Enric Aguilar

Bacteria depending on the age and the high OBJECTIVE To discover which is the most contaminated class by bacteria. To investigate the high where bacteria live better.

HYPOTPTHESIS We think there are more bacteria in kidâ&#x20AC;&#x2122;s class floor because the kids are closer to the ground.

EXPERIMENTAL DESIGN Material: - Petri dishes -

Tables Cupboards Oven Fixed variables

Method: - We put at the same time different petri dishes in different highs. Then we put the petri dishes in the oven and we wait 2 weeks and then we counted the number and the average of colonies.

Result

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Charts

Kid's

ground midle hight

Number of colonies 20 11 42

Diameter (average) 4,85 5,18 4,48

Primary

ground midle hight

19 21 28

7,05 7,71 5,11

ground Secondary midle hight

63 62 19

4 5,5 5,26

Graphs

Bateria in school

8 7 6 5 4

Ground

3 tr(vg) e iam D

table

2 1 0

cupboard

Kid's

Primary

Secondary

Diameter (average)

Age

Bacteria in school

70 60 50 40

is fcln ro e b m u N

ground

table

hight

0 Kid's

Primary

Secondary

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Number of colonies

Photos Secondary

Cupboard

Table

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Floor Primary

Cupboard

Table

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Floor Kids

Cupboard

Table

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Floor

Conclusions There are more bacteria in secondary class than in kid’s class. In secondary cupboard there less bacteria than primary and kid’s cupboard. There are more colonies on tables. On the floor there are less colonies than cupboards and tables. On kid’s table there are less colonies than primary and secondary tables.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Abril llabrés, Edna Buxaderes, Odette Estrella and Jenny Caicedo CLIL 2N ESO B 13/06/13

BACTERIA IN TOILETS 1. Objective To investigate the difference between the growth of bacteria in boys and girls toilets of different ages. 2. Hypothesis In the girls’ bathroom there are more bacteria because when we go to do our needs, we sit in the WC. 3. Experimental design 3.1 Material - A petri dish - The toilets - Students 3.2 Method We wrote on the petri dish each location. Then we put the petri dish in the toilet and we open it. We wait 15 minutes and then we take out and close the petri dish. After, we put that in the oven at 37º during fifteen days.

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4. Results 4.1. Photos

4.2. Charts

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4.3. Graphs BACTERIA IN TOILETS 30 25 20 BOYS 15

GIRLS

10 5 LIS FC O ER B M U N

0 SECONDARY AGE HIGH SCHOOL

BACTERIA IN TOILETS 8 6 BOYS 4

GIRLS

2 S LN FC O ETR M IA D

0 SECONDARY

HIGH SCHOOL

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BACTERIA IN TOILETS

S LN FC O ETR M IA D

8 7 6 5 4 3 2 1 0

BOYS GIRLS

SECONDARY

HIGH SCHOOL

5. Conclusions Our hypothesis was right because there are more bacteria in the gilrs’ toilets. We think there are more bacteria there because when we go to do our needs, we sit on the WC. The colonies in the petri dish of girls are bigger than the other colonies and there are more too. The girls’ toilets of secondary is the most contaminated and the boys’ toilets of high school is the less contaminated. So, boys in secondary are the dirtiest students.

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DO WE CLEAN WELL OUR HANDS? 1. Objective: · To check if bacteria grow more when you wash your hands with cold water, hot water, cold water+soap or hot water+soap. 2. Hypothesis: · We think that bacteria grow more when you wash your hands with cold water+soap. 3. Experimental design Material: · · · · · · · ·

Petri dishes Hot water Cold water Soap Permanent maker Oven Ruler Microscopy

Method: · · · ·

Firstly, one person of the group wash his hands with cold water and soap. Then, we put our fingers in the petri dishes. Later, we do the same, but with hot water+soap, only hot water and only cold water. Finish at all we have to wait for the results.

4. Results:

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BACTERIS IN THE WATER 500 400 with soap

300

Without soap 200 100 0 Hot Water

Cold Water

5. Conclusions: · In the first graph we can see that bacteria grow more in the cold water with soap and in the hot water with soap. · In the second and last graph we can see that the diameter of colonies with soap and cold water is higher than the hot water+soap. · In order to wash your hands as best as possible we recommend you to wash it with only hot water, because we have observed that it’s the best possible to get your hands clean. · We don’t recommend you at all to clean your hands with cold water and soap, because it’s the way you get your hands more dirtier. But we know that people don’t agree.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Emma Gallardo, Clàudia Codina, Khadija El Bouzi i Victor Rodriguez Ciències CLIL 2n ESO-C

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Txell Rubio, Joana Comes, Frida Yana, Maholy Espinoza, Laia Castany

Objective: To discover if saliva contains more bacteria than hands.

Hypothesis: We think that there are more bacteria in saliva than in hands.

Experimental design:

Materials: · · · · · ·

Room 4 sane of the same age. 2 females 2 males Ruler chronometer

Method: First we choose 4 persons, two female and two males. Then, one girl and one boy put saliva of their mouth in a different petri-dishes and wait for ten minutes. After that we did the same but with hands. Then we put the petri-dishes in the oven and wait for two weeks. Finally, after two weeks we took the petri-dishes out of the oven and we counted the number of colonies and the number of species of each petri-dish. We also measured the size of colonies. 4.

Results:

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Txell Rubio, Joana Comes, Frida Yana, Maholy Espinoza, Laia Castany

4,5 4

4 3,5 3

3 2,5

cis e Sp

Boy

Girl

1,5 1 0,5 0 Saliva

Body's parts

Hand

The boys have more species in the saliva of the girls and the girls have more species in the hands .

0,94

1 0,9

0,82

0,8 0,7 0,6 0,5

Boy

0,4

Girl

0,3 s ln trco e iam D

0,2 0,1

0,06

0,03

0 Saliva

Hand Body's parts

The girls have more species in the hands and the boys have more especies in saliva.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Txell Rubio, Joana Comes, Frida Yana, Maholy Espinoza, Laia Castany

100

90 80 70

60 50

Boy

Girl

30 is fcln ro e b m u N

20 7

10 0 Saliva

Hand Body's parts

The girls have more species in her body.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Txell Rubio, Joana Comes, Frida Yana, Maholy Espinoza, Laia Castany

Conclusions:

Girls have more especies of bacteria in her body than the boys. Girls have more especies in her hands. Boys have more especies in his salive. Girls have more bacteria in her hands and boys have more bacteria in his salive. Because girls often thaught themselves more t han boys. Our hypotesis was right, because we have checket that boys have got more bacteria in their salive. The diameter of the colonies of the girls is higher than the boys.

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BACTERIA IN TOILETS Objective: ü To discover the most contaminated toilet by bacteria in the school. ü To learn how to work with petri dishes. Hypothesis: The hypothesis is that the toilet of the break time is the dirtiest. Experimental design: 1. Material: · Petri dishes · Oven · Laptop · Mobile phone (to take photos) · Permanent maker · A ruler · Microscope · Calculator 2. Method: a. First of all, we took the petri dishes we wrote on them the location they went. b. We put the petri dishes in the different toilets during the same time at the same temperature. We waited 10 minutes and we collected them and put them in the oven for two weeks. c. Two weeks later, we took them out from the oven and counted: i. The number of colonies in each petri dish ii. The average of diameter of the colonies. iii. The number of different species. d. We observed the petri dishes in the microscope to see how they were. e. We took some photos of the petri dishes and saved them to the computer. f. Then we analyze data.

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Results: 1. Charts Toilets 2 d’ESO 3rd d’ESO 4th d’ESO Break time

Diameter (mm) 6,6 7,6 9,8 7,8

Number of colonies 15 3 6 5

Species of bacteria 9 3 5 4

2. Graphs

Bacteria in Escorial's toilets

16 14 12

10 8

6,6

9,8 7,6

Diameter (mm)

7,8

3 3

Number of colonies

5 4

2 0 2n of ESO

3rd of ESO

4th of ESO

Number of spiecies

Break time

3. Photos

In the photo we can see bacteria that are in the toilet of 3rd of ESO after being exposed for 15 minutes in the bathroom and after being 2 days in the oven.

In the photo we can see bacteria that are in the toilet of the Break Time after being exposed for 15 minutes in the bathroom and after being 2 days in the oven.

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In the photo we can see bacteria that are in the toilet of 2nd of ESO after being exposed for 15 minutes in the bathroom and after being 2 days in the oven.

In the photo we can see bacteria that are in the toilet of 4t of ESO after being exposed for 15 minutes in the bathroom and after being 2 days in the oven.

Conclusions: 1. Our first conclusion is that the toilet of 2nd of ESO is the dirtiest and it has the highest number of different species. 2. Our second conclusion is that the 3rd of ESO toilet is the cleanest. 3. We think that the 2nd of ESO is the dirtiest because they donâ&#x20AC;&#x2122;t clean the toilet as often as the other ones. 4. The toilet with the highest diameter of colonies is the toilet of 4th of ESO.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Neus Altarriba, Lizeth Moreno, Jennifer Orozco and Zineb Abdou Experiment review- CLIL 2º ESO A

1. Objective: Ø To investigate how many bacteria are on coins, on a pen, on a laptop and on a door handle and check what objects are more contaminated. 2. Hypothesis: Ø We believe that there are more bacteria on coins and on a door handle than on a pen or on a personal computer (laptop) because usually, many people take and touch these last two quotidian objects. 3. Material: ü Some coins ü A door handle ü A pen ü A laptop ü Adhesive ü Microscope ü Ruler ü Permanent marker ü Camera (mobile phone) ü Watch ü Petri dishes ü Oven 4. Method: Ø We put on a petri dish the coins and the pen. We did the same with the table and the laptop (in that case, we placed the petri dishes under them). At the same time, we put under of the door handle another petri dish. After fifteen minutes we took away the petri dishes and finally we put these in the oven at 37º degrees for two weeks approximately. Ø After two weeks we take the petri dishes out of the oven. In the class, we observe d each one, and measured the diameter of colonies. We also counted the number of colonies of each petri dish. We took photos of them and wrote the number in our notebooks. 5. Results Ø Charts: Nº of colonies Diameter of colonies Coins 41 18,2 Personal computer (laptop) 16 11,1 Door handle 20 10,7 Pen 50 15,6

6. Graphics:

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Neus Altarriba, Lizeth Moreno, Jennifer Orozco and Zineb Abdou Experiment review- CLIL 2º ESO A

Microorganisms in quotidian objects 50 45 40 35 30 25 20 15 10 5 0

Diameter (average) Number of colonies

Coins

Door handle

Pen

Ø We can observe that the pen is the object with the highest number of colonies. Ø We can observe that the coins are the object with the highest number of diameter colonies. Ø We can observe that the PC and door handle are the objects which contain the same number of colonies and diameter of colonies. Furthermore these two objects are less contaminated. 7. Photos: Photo of coins: Photo of PC:

Photo of door handle:

Photo of pen:

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Neus Altarriba, Lizeth Moreno, Jennifer Orozco and Zineb Abdou Experiment review- CLIL 2º ESO A 8. Conclusions: Ø When we saw the charts and the graphics, we realized that our hypothesis wasn’t correct, because the chart proves that the number of colonies that are in a pen (50) is superior than the numbers of colonies are in the door handle (20). Ø Now we know that on coins are 41 colonies of bacteria, on the personal computer (laptop) are 16 colonies, on the door handle are 20 colonies and on the pen are 50 colonies. Therefore, the most contaminate object is the pen, followed by coins, the door handle and the last is the personal computer (laptop).

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Oriol Riba, Aleix Raurell, Pol López, Andrea González

MICROORGANISMS IN THE SHCOOL OBJECTIVE: ·

To discover where bacteria are: In our class or in the kid’s class

HYPHOTESIS: Ø We believe that there are more bacteria in the kid’s class because they touch more objects than us. We believe that there are more bacteria in the ground that in other highs EXPERIMENTAL DESIGN: MATERIAL: Petri dishes Oven Microscope Permanent maker METHOD: We started writing on a petri dish its location with a permanent maker. They were at the fridge and then we take them to the classrooms of the kid’s and our classes. We wait 10 minutes and the teacher put on the oven one week the petri dishes. Finally take to as the petri dishes and we saw that the bacteria grow up and they form colonies.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Oriol Riba, Aleix Raurell, Pol López, Andrea González

RESULTS: Number of colonies

Kid’s class

Secondary class

Diameter of colonies

Highest

2,2

Middle

1,5

On the floor

2,14

Highest

0,1

Middle

2,4

On the floor

1,4

In the kid’s class there were more bacteria’s in the highest position, but there were minute’s bacteria’s than de secondary class.

Created by Simpo PDF Creator Pro (unregistered version) http://www.simpopdf.com Oriol Riba, Aleix Raurell, Pol López, Andrea González

In this graphic we can see, that the number of colonies is very high, and the diameter is very low.

In this graphic we can see that there are more bacteria’s in the secondary class. In the secondary class there are more bacteria’s in the ground and in the highest position and in the kid’s class there are more bacteria’s in the highest position. CONCLUSIONS: We can say that in the secondary class there are a lot of more bacteria are that in the kid’s class. In secondary class there are menus bacteria’s in the middle position and in the highest and on the floor there are the same number of bacteria’s. In the kid’s class, there are more bacteria’s in the highest position and in the middle there are menus bacteria’s. We thought that in the kid’s class there will be more bacteria’s than in the secondary class.