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Automated Liquid Handling Product Guide Automated Liquid Handling | Product Guide

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Featuring the NEW

Gilson, Inc. World Headquarters | P.O. Box 620027 | Middleton, WI 53562-0027 | Tel: 800-445-7661 | Fax: 608-831-7661 Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE | Tel: 33-(0)1-34295000 | Fax: 33-(0)1-34295020 www.gilson.com | sales@gilson.com | service@gilson.com | training@gilson.com | hr@gilson.com | oem@gilson.com

PLC 2020

Personal Purification System (See pages 3– 4)

LT303041-11 © 2011 Gilson Inc. All rights reserved.

All trademarks are the property of Gilson Inc. and its subsidiaries.

Table of Contents

Contents

About Gilson, Inc. Designed for purification and engineered for science, Gilson, Inc. provides high quality, dependable solutions for today’s demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation chromatography clean-up requirements.

From the legendary Gilson Pipetman® products to a full range of system solutions, Gilson is the element of purification for your laboratory. The Gilson line of innovative products will revolutionize the lab; providing the durability, flexibility, modularity and ease-of-use the industry has come to expect from Gilson.

Gilson has worldwide sales and service supporting the pharmaceutical, biotech, environmental, clinical, food and forensic markets. Our primary focus is on purifying samples via preparative HPLC, automated SPE or through GPC clean-up. Gilson focuses on early drug discovery and development with our preparative HPLC systems. Gilson automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic markets performing a wide variety of clean-up applications.

Gilson offers complete solutions including the revolutionary control software, Trilution®.

Trilution® LC Software is a complete HPLC software package designed specifically to meet purification needs. Trilution® LH Software is designed to make automation of liquid handling and SPE applications simple and dependable.

Gilson, Inc. world headquarters is located in Middleton, WI and is responsible for research, development and manufacturing of all Gilson instrumentation and software in addition to providing direct sales to the U.S. Gilson S.A.S., located outside of Paris, France, is responsible for all development and manufacturing of Gilson pipetting products and benchtop laboratory products. Gilson solutions are sold and supported worldwide.

PLC 2020 Purification System

3

LC/MS Purification System

5

HPLC Systems

7

Gilson HPLC Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 GX-281 Preparative HPLC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 GX-281 Analytical to Semi-preparative HPLC System . . . . . . . . . . 10 GX-271 Preparative HPLC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 GX-271 Analytical to Semi-preparative HPLC System . . . . . . . . . . 12 845Zprep™ Semi-preparative to Preparative HPLC System . . . . . 13 cLC™ Analytical to Preparative HPLC System . . . . . . . . . . . . . . . . . . . 13 GX-271 Fraction Trapping System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

HPLC Liquid Handlers & Injectors GX-281 Liquid Handler/Injector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

GX-271 Preparative Liquid Handler/Injector . . . . . . . . . . . . . . . . . . .

GX-271 Analytical Liquid Handler/Injector . . . . . . . . . . . . . . . . . . . . .

215 Liquid Handler/Injector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

HPLC Pumps

15 16 17 18 19

20

333/334 Prep-scale HPLC Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 331/332 Semi-preparative HPLC Pumps . . . . . . . . . . . . . . . . . . . . . . . . 22 321/322 Analytical to Semi-preparative HPLC Pumps . . . . . . . . . 22 30X Series HPLC Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 402 Syringe Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Minipuls Evolution® Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Minipuls 3 Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

HPLC Detectors

25

171/172 Diode Array Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 15X Series UV/VIS Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 112 UV Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 prepELS™ II Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

HPLC Fraction Collectors

28

42

Liquid Handlers GX-271 Analytical Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

GX-271 Preparative Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

GX-274 Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

GX-281 Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Quad-Z 215 Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

215 Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

223 Sample Changer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

43 44 45 46 47 48 48

49

Accessories VALVEMATE® Valve Actuator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Orbital Shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

RackCount™ System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

818 AutoMix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Racks – Standard & Custom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Manual Liquid Handling PIPETMAN® M . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PIPETMAN® Neo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PIPETMAN® Neo Starter Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PIPETMAN® Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Trackman® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

50 51 51 52 52

53 53 55 55 57 61

63

Resources

OEM Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 Gilson Knowledge Center Gilson Publications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 Technical Notes Handbooks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Gilson Applications Environmental Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Food & Beverage Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Clinical & Forensic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Pharmaceutical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

References

67 67 68 69 70

71

Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 FC 203B/FC 204 Fraction Collectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

prepFC™

Control Software

31

Trilution® LC Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Trilution® LH Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

GPC Clean-up Systems

34

Automated GX-271 GPC Clean-up System . . . . . . . . . . . . . . . . . . . . . 35 GX-271 GPC/SPE Clean-up System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 Manual GPC Clean-up System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Solid Phase Extraction Systems

38

GX-271 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 GX-274 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Quad-Z 215 Liquid Handler for SPE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Gilson, Inc. World Headquarters | P.O. Box 620027 | Middleton, WI 53562-0027 | Tel: 800-445-7661 | Fax: 608-831-7661 Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE | Tel: 33-(0)1-34295000 | Fax: 33-(0)1-34295020 www.gilson.com | sales@gilson.com | service@gilson.com | training@gilson.com | hr@gilson.com | oem@gilson.com

Gilson, Inc. | 800-445-7661 | www.gilson.com

2

Easy-to-Use Software Specifications

LC in a Box! A fully functioning purification system in a small, compact footprint, the PLC 2020 Personal Purification system was developed in response to the growing need for an easy-touse, self contained system to handle a chemists purification needs on a more personal level. The PLC 2020 Personal Purification System is ideally suited for low-throughput, high pressure purification needs and is intended to support an individual or small group of chemists.

Injection Loop Volume 20 µL – 10 mL Flow Rate Maximum Pressure 1 – 100 mL/min 4060 psi (depending on pump head) Solvent Selection Detection Up to 4 solvents for B pump UV/VIS Dual Wavelength 190 –700 nm

Performs various techniques: FLASH Normal Phase Reverse Phase

Storage for up to four 4L solvent bottles.

Superior resolution to FLASH Save time—No need to purify the sample multiple times to achieve required purities.

Running the System

Simple as:

Tap Run on the main menu

Intuitive touch screen interface allows you to be purifying compounds in minutes.

Real-time, graphical gradient modifications and real-time sample tracking during run.

Conditional logic fraction collection based on up to two detector channels using any combination of Slope and/or Level.

Auto-Print and Auto-Export of sample reports at the end of a run.

Column holders for FLASH & Reverse Phase columns.

Integrated touch screen control.

3 rack positions dedicated to fractions.

Select Method and tap Run

Monitor the progress of the system without being next to it as run lights illuminate the racks during a run, turn off when a run is complete and FLASH if an error has occurred.

Load and inject sample

NEW! PLC 2020 Purification System

PLC 2020 Purification System

FLASH

3 USB ports for easy download/upload methods and results, via flash drive. Easily connect your printer, keyboard and mouse.

Optimize your flow rate range with interchangeable pump heads 25, 50 and 100 mL/min.

FLASH Dry Load

Up to 4 solvents for B pump.

Run Method - FLASH Dry Load 100

2000 1750

Waste Waste Mobile Phase To FLASH

Interchangeable pump head design to accommodate wide flow rate and pressure ranges.

Selection of up to 4 different solvents.

Product #: 21030000 (PLC 2020 Personal Purification System)

3

Automated Liquid Handling | Product Guide

% Mobile Phase

To FLASH

60 40 20 0

Detector

1250 1000 750 500 250 0

Mobile Phase Detector

1500

mVolts

80

-250

0

Time (minutes)

11.0

Accommodates any FLASH style cartridge as well as dry load configurations.

High pressure Reverse Phase columns.

Gilson, Inc. | 800-445-7661 | www.gilson.com

4

It’s Your Chemistry... Simplified!

Your time is valuable. The quality of your data is crucial. We understand you need simple, fast and flexible solutions that allow you to focus on your research not your equipment. That’s why Gilson has developed an LC/MS Purification System that is not only extremely easy-touse and easy-to-maintain, but has one of the largest mass ranges on the market today.

Your specifications Modular design means each component can be configured to your specifications.

NEW! LC/MS Purification System

LC/MS Purification System

MS Detector Features & Benefits Quick-connect

Rugged & Simple

Quick-connect feature allows each user to have their own probe, preventing contamination between users in open access environments.

Rugged and simple to maintain source allows for cleaning and maintenance without breaking vacuum—limiting instrument down time and increasing productivity.

Multiple Mode Operation

Automation & Calibration

Ability to monitor in Full Scan, Extracted Ion and SIM (Selected Ion Monitoring) simultaneously.

Integrated calibration vials for Auto-Tune allows for automated and effortless mass calibration.

Your own probe Just walk up and snap in to start, without worry of cross contamination in an open access, multi-user environment.

Your choice Choose to collect fractions using conditional logic based on any combination of UV, DAD, ELSD and up to seven MS Channels.

Source Features The source features XYZ adjustments of probe positioning to optimize spray in relation to the capillary entrance for higher flows and/or sample concentrations.

Range & Speed Product #: 18100003 (MS, PE FLEXAR SQ300 W/SRNG & DIV VLV)

Trilution® LC Software

Mass Range 50 – 3,000 amu Scans Speeds up to 10,000 amu/sec

Simple and Specifically Designed for Preparative Purification Complete and seamless integration into Trilution® LC Software allows for MS control in a single software package specifically designed for purification.

Intuitive, easy-to-use drag and drop method interface.

Mass Based Purification

Simple as:

Unmatched fraction collection capabilities with Slope, Level, Time and Volume collection.

Select scan range

Real-time spectrum display of each fraction as they are collected means you wont need to wait for post-run analysis.

Application Run screen with real time fraction spectra

Active Splitter Active Splitter allows for large, setable split ratio to allow the system to operate with a large flow range and column conditions.

Enter target masses and adducts

Split ratio is independent HPLC of Stream changes in the HPLC flow

Select conditional logic collection to trigger off both UV and MS

Separate make up pump dilutes and provides a constant, user setable flow rate to the MS independent of HPLC flow rate (0.010 to 1.5 mL/min).

RUN Mass Spec Single Quad Settings

5

Automated Liquid Handling | Product Guide

1HPLC Stream

Mass to be Mass to be Transferred Transferred 1

3

1HPLC Stream

Mass Spec Mass Spec Stream 3 Stream

4 2

HPLC Stream

3

Aliquot Fill

2

Mass Spec Mass Spec Stream 3 Stream Mass Mass Transferred 4 Transferred

4

4 2

1

2

Product #: 3602671 (307 Pump)

Aliquot Transfer

Gilson, Inc. | 800-445-7661 | www.gilson.com

6

Gilson HPLC Systems Gilson high performance liquid chromatography (HPLC) systems provide complete solutions for all purification needs. Front-end liquid handlers and autosamplers are available in a range of bed capacities and optimized bench space footprints.

All models accommodate innovative injection and fraction collection capabilities within a single platform. Trilution® LC Software completely controls the entire HPLC system, allowing focus to remain on the science rather than the software.

Analytical, Semi-Prep, to Preparative Purification The Gilson line of complete system solutions provide chromatography systems that range in flow rates from 0.01 mL/min up to 200 mL/min and are capable of integrating the entire range of chromatography techniques from Reverse Phase to Ion Exchange.

Quick Comparison Chart

Column ID 150 mm

100 mm

50 mm

30 mm

20 mm

10 mm

4.6 mm

2 mm

Page #

3

LC/MS

Flow Rate 1–200 mL/min Injection Loop Volume 100 µL– 25 mL Mass Range 50 – 3,000 amu Sample Mass 300 mg/injection

5

GX-281 Preparative HPLC System

Flow Rate 1 – 200 mL/min Injection Loop Volume 100 µL – 25 mL Sample Mass 15 g/injection

9

Flow Rate 0.15 – 30 mL/min Injection Loop Volume 2 µL – 2 mL Sample Mass 300 mg/injection

10

Flow Rate 1 – 200 mL/min Injection Loop Volume 100 µL – 25 mL Sample Mass 15 g/injection

11

GX-271 Analytical to Semi-preparative HPLC System

Flow Rate 0.15 – 30 mL/min Injection Loop Volume 2 µL – 5 mL Sample Mass 300 mg/injection

12

845Zprep™ Semi-preparative to Preparative HPLC System

Flow Rate 1 – 200 mL/min Injection Loop Volume 100 µL – 10 mL Sample Mass 15 g/injection

13

GX-281 Analytical to Semi-preparative HPLC System GX-271 Preparative HPLC System

7

Specifications Flow Rate 1–100 mL/min Injection Loop Volume 20 µL– 5 mL Sample Mass 300 mg/injection

PLC 2020

cLC™ Analytical to Preparative HPLC System

Flow Rate 1 – 200 mL/min Injection Loop Volume 5 µL – 25 mL Sample Mass 15 g/injection FLASH chromatography

13

GX-271 Fraction Trapping HPLC System

Flow Rate 25 – 40 mL/min Injection Loop Volume up to 25 mL Aspiration Flow Rate 5 mL/min for fraction loading

14

Automated Liquid Handling | Product Guide

HPLC » Systems

HPLC Systems

Gilson VALVEMATE® II Valve Actuator offers multiple column selection availability featuring: fast switching times, high pressure valve control of 6 or 10-port, 2, 6 or 10-position and low pressure valve control of 6 or 8-positions. (see page 50)

Gilson accurate pumping solutions are available with multi-solvent selection, high pressure variable mixing and interchangeable pump heads. (see pages 20 – 24)

Gilson GX-281 Liquid Handler is the premier front-end instrument featuring a large bed capacity and a wide array of components such as automatic reinjection of fractions using innovative GX Z-Injection technology. (see page 46)

The GX-271 Liquid Handler, is a mid-size capacity and throughput instrument fulfilling numerous laboratory needs at an economical price. (see page 43)

The 215 Series Liquid Handlers, known as the workhorses of the industry, are available in single, multiple and variable spanning probe models. (see page 48)

The Gilson variety of detection solutions offer excellent sensitivity and reproducibility including diode array (DAD), ultraviolet (UV) and/or visible (VIS) and evaporative light scattering (ELS) detection. (see page 25 – 27)

Gilson, Inc. | 800-445-7661 | www.gilson.com

8

The GX-281 Preparative HPLC System allows for injection, fraction collection and reinjection. The system is also ideal for liquid handling techniques such as sample mixing, dilutions and sample extract transfers.

The GX Z-Injection Module allows sample aspiration directly into the valve without a separate injection port, eliminating a source of potential carryover.

Specifications Sample Loading Capacity 15 g/injection Injection Loop Volume 100 µL–25 mL

Automated Liquid Handling | Product Guide

Maximum Injection Pressure 3,000 psi (207 bar)

Flow Rate 1–200 mL/min

Maximum Pump Pressure 3,040 psi (210 bar)

GX-281 Analytical to Semi-preparative HPLC System The GX-281 is a multi-functional instrument that can perform injection, fraction collection and reinjection.

The GX Z-Injection Module allows fast injections with low carryover and low maintenance by aspirating the sample directly into the valve.

X Direct Injection technology G features an injection port that connects directly to the valve, minimizing volume associated with connective tubing (located behind the racks).

The GX Prep Solvent System features a dynamic range of volumes and flow rates using syringeless technology within a solvent delivery pump capable of accessing five off-bed solvents.

9

Optimal Column Size 4.6–100 mm

HPLC » Systems

GX-281 Preparative HPLC System

Specifications Sample Loading Capacity 300 mg/injection

Injection Loop Volume 2 µL – 2 mL

Optimal Column Size 2 – 20 mm

Flow Rate H1: 150 µL/min – 15 mL/min H2: 30 µL/min – 30 mL/min

Maximum Injection Pressure 5,000 psi (345 bar)

Maximum Pump Pressure H1: 8,700 psi (600 bar) H2: 4,350 psi (300 bar)

Separate injection and fraction collection paths reduce cross-contamination of fractions from sample injection. The cycle time of the injection is minimized by eliminating rinse steps which are required with single fluid path configurations.

The prep-scale HPLC pumps feature low-dwell volumes which allow for fast, reproducible gradients. (see page 21)

The GX-281 features a Jet Wash Rinse station design that delivers individual streams of solvent for each separate rinse station to actively wash the outside of probes. Four rinse solvents are available for selection.

Semi-preparative HPLC pumps are offered to perform binary gradients. (see page 22)

Detection solutions are available for DAD, fixed or variable wavelength UV/VIS, UV, MS and ELS detection. (see page 25)

Gilson, Inc. | 800-445-7661 | www.gilson.com

10

A single, mid-size instrument for injection and fraction collection with separate fluid pathways to reduce cross-contamination of fractions.

Specifications Sample Loading Capacity 15 g/injection Optimal Column Size 10 – 100 mm Maximum Injection Pressure 3,000 psi (207 bar)

Injection Loop Volume 100 µL – 25 mL Flow Rate 1 – 200 mL/min Maximum Pump Pressure 3,040 psi (210 bar)

GX-271 Analytical to Semi-preparative HPLC System Specifications The GX-271 is a mid-size liquid handler/fraction collector with a bench-saving footprint featuring separate fluid pathways that reduce crosscontamination of samples and fractions.

The GX Analytical Solvent System features innovative syringeless technology and access to five off-bed solvents.

Mid-to-large collection capacity in a small footprint.

Automated Liquid Handling | Product Guide

Injection Loop Volume 2 µL – 5 mL Flow Rate H1: 150 µL/min – 15 mL/min H2: 30 µL/min – 30 mL/min Maximum Pump Pressure H1: 8,700 psi (600 bar) H2: 4,350 psi (300 bar)

Optimal Column Size 2 – 20 mm Maximum Injection Pressure 5,000 psi (345 bar)

The VALVEMATE® II Valve Actuator can be incorporated for multiple column selection or column regeneration applications. (see page 50)

11

Sample Loading Capacity 300 mg/injection

Binary gradient preparative HPLC pumps provide high-flow capabilities. (see page 21)

The GX Prep Solvent System uses syringeless technology and allows access to five off-bed solvents.

The GX Direct Injection Module can utilize both 1/16" and 1/8" injection valves, allowing for a wide variety of sample volumes and loop options.

HPLC » Systems

GX-271 Preparative HPLC System

Binary gradients are achieved with analytical to semi-preparative HPLC pumps. (see page 22)

For confirmation or added detection capabilities of non-chromaphoric compounds configure an additional ELS detector with a multi-channel UV or DAD detector. (see page 25)

Modular detector options available. (see page 25)

High mount collection valve for collecting to small targets such as microplates and sealed vessels.

The low mount collection valve enables fraction collection to tubes via a separate fluid path from the injection.

Gilson, Inc. | 800-445-7661 | www.gilson.com

12

Gilson 845Zprep™ System features the first generation Z-Injection technology accommodating volumes and flow rates for semi-preparative to preparative applications.

Mid-to-large bed capacity, accommodating both samples and fractions.

HPLC » Systems

845Zprep™ Semi-preparative to Preparative HPLC System

GX-271 Fraction Trapping System

Specifications Sample Loading Capacity 15 g/injection

Maximum Injection Pressure 3,000 psi (207 bar)

Optimal Column Size 4.6–50 mm

Maximum Pump Pressure 3,040 psi (210 bar)

Injection Loop Volume 100 µL–10 mL

Carryover < 0.01%

Injection Variability < 1.5%

Flow Rate 1 – 200 mL/min

The Gilson revolutionary Fraction Trapping System is unlike any other system on the market today.

The Fraction Trapping System is ideally suited for processing fractions up to 20 mL in volume and concentrations up to 500 mg that have been purified on virtually any preparative HPLC system.

Washing steps can be performed for removal of salts, buffers and mobile phase modifiers such as TFA that can hinder further LC/MS analysis.

Fractions containing greater than even 50% water content can be processed and collected in 100% of organic solvent. This allows for fast dry down times at lower temperatures for samples that are heat sensitive. Dry down times of 12 – 24 hours can be reduced to less than 30 minutes.

Steps involved in Fraction Trapping TRAP – Sequestration from fraction of desired solute(s) by a solid phase.

RINSE – Removal of buffers, modifiers, salts, etc. by appropriate rinsing of the solid phase and the ‘trapped’ solute(s).

ELUTE – ‘Trapped’ solute(s) desorbed to produce a relatively small volume of ‘dry’ organic solvent solution of the desired solute.

Trapping Column Specifications Trapping Columns

Recommended Recommended Load Capacity Flow Rate

10 mm ID x 150 mm

200 mg

25 mL/min

21.2 mm x 150 mm

500 mg

40 mL/min

Process fractions in 8 to 12 minutes with volumes up to 20 mL and concentrations up to 500 mg.

Optional breakthrough detector and secondary fraction collector.

Specifications Flow Rate 50 mL/min

Wavelength 190 – 700 nm Aspiration Injection Loop Flow Rate Volume 5 mL/min for up to 25 mL fraction loading Detector Variable Dual Wavelength

cLC Analytical to Preparative HPLC System ™

Gilson cLC™ System is a complete chromatography system capable of automating the purification of compounds using both normal and reversedphase columns. The cLC System has the ability to accommodate various modes of detection including DAD, UV/VIS, ELS and RI. Additional analytical components are also integrated onto the cLC System enabling automated on-line evaluation of the collected fractions for purity and recovery.

Specifications Sample Loading Capacity 15 g/injection Optimal Column Size 4–100 mm Maximum Injection Pressure 5,000 psi (345 bar)

Injection Loop Volume 5 µL–25 mL Flow Rate 1–200 mL/min Maximum Pump Pressure 33X Pump: 3,040 psi 32X Pump: 8,700 psi

Product #: 21120001 (GX-271 Fraction Trapping System)

App: Standard FTLC Methods - Method: FTLC GX271 Prep Injector 180 100

40

60 30 0

0

Automated Liquid Handling | Product Guide

Fraction Zone, 2

90 60

20

13

UV Data 1 UV Data 2

120

mVolts

% Mobile Phase

80

150

-30 -60

0

2

4

6 8 Time (minutes)

10

12

14

16

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14

GX-281 Liquid Handler/Injector Specifically engineered for semi-preparative to preparative HPLC requirements, the GX-281 offers unmatched versatility and performance for front-end LC automation including revolutionary syringeless pumping technology.

GX-281 Liquid Handler/ Injector



100 µL – 25 mL*

98% (100 µL – 25 mL)

< 0.005%

16

Injection Carryover Dimensions (w x d x h) < 0.005% 87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in)

Fraction Zone, 11 Fraction Zone, 12



Injection Page Carryover #

Fraction Zone, 1 Fraction Zone, 2 Fraction Zone, 3 Fraction Zone, 4 Fraction Zone, 5 Fraction Zone, 6

Liquid Handling Accuracy

Sample Quantity 576 576 450 420 180 162

mVolts

Injection Loop Volume

Tube Diameter 13 mm 15 mm 16 mm 18 mm 25 mm 28 mm

Maximum Injection Pressure 5,000 psi (345 bar)

Injection, Collection and Analysis

% Mobile Phase

Quick Comparison Chart Fraction Collection

Injection Loop Volume 100 µL – 25 mL Accuracy 98% (100 µL – 25 mL)

GX-281 Liquid Handler/ Injector Bed Capacity

Flow Rate 1 – 200 mL/min

Fraction Zone, 9 Fraction Zone, 10

Gilson liquid handlers and injectors offer the broadest range of instrument capabilities on the market today. From small footprint injectors to larger footprint liquid handler/injector/fraction collectors, Gilson provides the ultimate front-end automation solutions for every HPLC system.

Specifications Reproducibility < 1% CV (total loop) < 3.5% (partial loop)

Fraction Zone, 7 Fraction Zone, 8

Front-End Automation Solutions

Liquid Handler

HPLC » Liquid Handlers /Injectors

HPLC Liquid Handler/Injectors

Note: tubes up to 220 mm tall can be accommodated

GX-271 Preparative Liquid Handler/ Injector



GX-271 Analytical Liquid Handler/ Injector





2 µL – 2 mL

98% (10 µL – 25 mL)

< 0.05%

18

215 Liquid Handler/ Injector





5 µL – 10 mL

99.7% (50 µL)

< 0.1%

19

Time (minutes)



100 µL – 25 mL*

98% (50 µL – 25 mL)

< 0.05%

17 Immediate response aspiration and dispensing with the GX Prep Solvent System eliminates time spent waiting for valves to switch and syringes to fill.

Two unique and separate liquid pathways for injection and fraction collection provide a zero-contamination environment.

The GX Direct Injection Module features an injection port that connects directly to the injection valve for decreased dead volume and reduced maintenance.

* Larger loop volumes available upon request.

Product #s: 261030 (GX-281 Liquid Handler) 261031 (GX-281 Liquid Handler with bar code) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK) 261353 (GX Z-Injection Module)

15

Automated Liquid Handling | Product Guide

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16

GX-271 Analytical Liquid Handler/Injector

The GX-271 Liquid Handler/Injector is the perfect blend of size, performance and capacity, consuming only 23” of linear bench space.

Large-bore injection modules feature a continuous flow path design for preparative applications.

Small-bore valves are available for analytical to semi-preparative applications with inert injection modules for bio-compatible applications.

The GX-271 Liquid Handler/Injector can be configured for large injection loop volumes with minimal sample waste and carryover as well as to accommodate either the GX Prep or Analytical Solvent System which feature syringeless pumping technology.

Specifications

GX-271 Preparative Liquid Handler/Injector Flow Rate 1 µL/min – 50 mL/min

Reproducibility < 1% CV (total loop)

Injection Loop Volume 100 µL – 25 mL

Maximum Injection Pressure 5,000 psi (345 bar)

Injection Carryover < 0.05%

Accuracy 98% (50 µL – 25 mL)

100 50

20

Jet Wash Rinse technology provides flowing rinse for fast and efficient rinsing protocols utilizing up to two separate flowing rinse solvents.

0 -50

0

The GX Direct Injection Module increases performance by incorporating a port that connects directly to the injection valve, reducing sample waste and maintenance.

mVolts

Fraction Zone, 3

150

Fraction Zone, 2

200

% Mobile Phase

Theophylline

Ethernet based communication provides better connectivity.

Fraction Zone, 1

40

Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

Phenol

250

mVolts

% Mobile Phase

60

Injection Carryover < 0.05%

Maximum Injection Pressure 5,000 psi (345 bar)

Series of Ten 20 µL Total Loop injections of Theophylline, Caffeine and Phenol

300

80

Reproducibility < 1% CV (total loop) < 3.5% (partial loop)

Flow Rate 1 µL/min – 5 mL/min

Accuracy 98% (10 µL – 25 mL)

Recovery Data: Theophylline (5 mg/mL), Caffeine (5 mg/mL) and Phenol (3 mg/mL) Solution with 1 mL Injection Loop Volumes calculated from reinjection of collected Theophylline fractions (Zone 2) 100

Specifications

GX-271 Analytical Liquid Handler/Injector

Injection Loop Volume 2 µL – 2 mL

Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

HPLC » Liquid Handlers /Injectors

GX-271 Preparative Liquid Handler/Injector

Caffeine

Time (minutes)

0

1

2

Time (minutes)

3

4

5 Separate fraction collection flow paths prevent contamination from the injection process.

Injection 1 2 3 4 5 6 7 8 9 10 Average Recovery

Recovery 99.86% 96.61% 98.22% 96.64% 100.10% 98.99% 99.57% 99.69% 99.81% 100.36% 99.99%

Standard Deviation

0.01

CV

1.39%

Available in an analytical or prep model, the solvent systems feature dynamic flow rate ranges and can access five off-bed solvents.

Peak

Retention Time (minutes)

Area Count (µL V min/100)

Standard Deviation

CV

Theophylline Caffeine Phenol

2.8

14901136.11

44928.68

0.30%

4.2 8.4

12267689.70 13314605.43

36503.01 73087.21

0.30% 0.55%

GX-271 Preparative and Analytical Liquid Handler/Injector Bed Capacity Tube Diameter

Sample Quantity

10 mm

540

13 mm

300

15 mm

220

16 mm

220

17 mm

220

18 mm

220

28 mm

70

Note: tubes up to 200 mm tall can be accommodated

Product #s: 2614107 (GX-271 Preparative Liquid Handler) 2614108 (GX-271 Analytical Liquid Handler) 2614101 (GX-271 Liquid Handler without pump) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK)

17

Automated Liquid Handling | Product Guide

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18

HPLC Pumps

215 Liquid Handler/Injector The 215 Liquid Handler/Injector, a true and trusted laboratory workhorse for many years, performs injection, fraction collection and reinjection of collected fractions within a single platform.

Unsurpassed Flexibility for All Types of Pumping Applications Gilson offers unsurpassed flexibility in HPLC pumping systems with an extensive product range and accommodations.

This versatile, large bed liquid handler accommodates standard and custom racks to hold virtually any sample vessel.

>50 mm

20 mm

10 mm

Automated Liquid Handling | Product Guide

4 mm

19

Column ID 2 mm

Reproducibility liquid handling: 1.37% CV (50 μL) injection: < 0.9% (1 mL) (total loop) Accuracy Injection Carryover liquid handling: 99.7% (50 μL) < 0.1% Injection Loop Volume Dimensions (w x d x h) 5 μL – 10 mL 91 x 61 x 56 cm (36 x 24 x 22 in)

Product #s: 2510121 (215 Liquid Handler) 251511 (819 Injection Module) 251551 (845Z Injection Module) 251552 (845Zprep™ Injection Module)

Quick Comparison Chart 1 mm

Specifications

Customize by adding accessories and components to meet laboratory needs (shown with 819 injection module).

Page #

Flow Rate

Pressure

333/334 Prep-scale HPLC Pumps

1 – 200 mL/min

3,040 psi (210 bar)

21

331/332 Semi-preparative HPLC Pumps

0.5 – 50 mL/min

8,700 psi (600 bar)

22

321/322 Analytical to Semi-preparative HPLC Pumps

H1: 0.15 – 15 mL/min H2: 0.3 – 30 mL/min

H1: 8,700 psi (600 bar) H2: 4,300 psi (300 bar)

22

30X Series HPLC Pumps

10 µL/min – 200 mL/min

8,700 psi (600 bar)

23

402 Syringe Pumps

1 µL/min – 120 mL/min

110 psi (8 bar) for 0.1 – 10 mL syringes 40 psi (3 bar) for 25 mL syringe

23

standard: 0.2 µL/min – 70 mL/min

standard: 42 psi (3 bar)

high-flow: 1 – 340 mL/min

high-flow: 29 psi (2 bar)

standard: (1, 2, 4 or 8 channels): 0.3 µL/min – 30 mL/min

standard: 72 psi (5 bar)

Minipuls Evolution® Peristaltic Pump

Minipuls 3 Peristaltic Pump

high-flow: (2 or 4 channels): 1 – 220 mL/min

high-flow: 42 psi (3 bar)

24

24

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20

331/332 Semi-preparative HPLC Pumps

Capable of fast separations with high-efficiency columns in normal and reversed-phase modes, Gilson 333 and 334 Pumps are the premier solvent delivery solutions for preparative chromatography. Choose the 333 master pump for isocratic applications or the 333/334 binary system for composition gradient with high pressure mixing (third pump for ternary).

Gilson 331 and 332 dual-piston pumps for semipreparative and preparative chromatography feature a variable volume, dynamic mixer for optimized gradient performance.

Stackable to conserve bench space. apable of milligram-to-gram level C mass-throughput per injection up to 15 g depending on column size and load capacity.

S mooth linear transposition from analytical to preparative HPLC methods using 4 to 20 mm ID columns packed with highefficiency stationary phases.

Trilution® LC Software provides control within a complete semi-preparative system including data processing and result reporting or standalone control via the keypad incorporated on the 331 Pump.

Specifications Flow Rate Accuracy 1 – 200 mL/min (3,040 psi) ± 1% or 5.3 μL/min Reproducibility < 0.5% or 2.7 μL/min RSD Dimensions (w x d x h) 333: 26 x 41 x 51 cm (10 x 16 x 20 in) 334: 26 x 41 x 39 cm (10 x 16 x 15 in)

S election of up to four solvents per pump for a total of eight solvents in a binary pumping system.

300

100

250

80

200

60

150

40

100

20

50

0

0

0

5

10 15 20 Time (minutes)

25

30

Detected Composition (mAU)

Programmed Composition (% E)

Gradient Linearity and Accuracy

321/322 Analytical to Semi-preparative HPLC Pumps igh pressure Adjustable Volume Dynamic Mixer H (AVDM™) enables fast gradient response times and homogeneous mixtures for analytical to semipreparative applications.

100 80 60 40 20 0

2

4 Time (minutes)

6

8

H2 pump heads are ideal for 4 to 20 mm ID columns.

CV

Methyl PB

Ethyl PB

Propyl PB

3.44

4.85

6.08

0.02

0.02

0.02

0.72%

0.34%

0.29%

Automated Liquid Handling | Product Guide

A single 321 or 322 Pump is capable of binary gradients.

Flow Rate H1: 0.15 – 15 mL/min (8,700 psi) H2: 0.3 – 30 mL/min (4,300 psi)

Product #s: 38103331 (left) (333 Pump, H3 primary solvent, dual-piston, reciprocating master pump) 38103341 (right) (334 Pump, H3 secondary solvent, dual-piston, reciprocating remote controlled pump)

21

While the pumps are controlled via Trilution® LC, the 321 also incorporates a keypad that allows independent stand-alone gradient control.

Specifications

0

Series of Ten Injections

Average Retention Time Standard Deviation

S olvent selection on each pump with optional 4-solvent valve for up to eight solvents.

Flow Rate Accuracy 0.5 – 50 mL/min (8,700 psi) ± 2% or 13 μL/min Reproducibility H2: ± 0.7% or 7 μL/min Dimensions (w x d x h) 331: 26 x 41 x 51 cm (10 x 16 x 20 in) 332: 26 x 41 x 39 cm (10 x 16 x 15 in)

Product #s: 38103312 (right) (331 Pump, H2 primary solvent, dual-piston, reciprocating master pump) 38103322 (left) (332 Pump, H2 secondary solvent, dual-piston, reciprocating remote controlled pump)

% Mobile Phase

mVolts

The flow rate can be doubled with additional parallel pumps to 400 mL/min.

The 321 and 322 multi-solvent pumps are designed for use with Gilson HPLC analytical and semi-preparative chromatography systems.

Retention Time Repeatability 210 180 150 120 90 60 30 0 -30

illigram-level sample massM throughput per injection; 150 mg with a 20 mm bore column.

Specifications The entire preparative HPLC system is controlled with Trilution® LC Software, additionally the 333 model has an incorporated keypad for stand-alone operation.

120

HPLC » Pumps

333/334 Prep-scale HPLC Pumps

Accuracy ± 2% or 13 μL/min

Reproducibility H1: < 0.7% or 2 µL/min H2: 4 µL/min Dimensions (w x d x h) 321: 26 x 41 x 51 cm (10 x 16 x 20 in) 322: 26 x 41 x 39 cm (10 x 16 x 15 in)

Product #s: 38103211 (321 Pump, H1) 381032211 (left) (322 Pump, H1) 38103212 (right) (321 Pump, H2) 381032221 (322 Pump, H2)

H1 pump heads are ideal for 2 to 10 mm ID columns.

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22

Flow Rate 305/306: 10 μL/min – 200 mL/min 307: 10 μL/min – 10 mL/min

The 305 and 307 Pumps are programmable through the incorporated keypad.

Accuracy < ± 1%

Reproducibility < 1% Dimensions (w x d x h) 33 x 33 x 15 cm (13 x 13 x 6 in)

The 307 model functions as an isocratic pump with a built-in manometric module.

Dynamic mixers ensure efficient, dependable gradient creation.

Minipuls Evolution® Peristaltic Pump

Specifications

Together with Gilson manometric modules and dynamic mixers, the 305, 306 and 307 Pumps offer a cost-effective, space saving and robust solution for isocratic or gradients of analytical to preparative HPLC.

Tools are not required for pump head removal, allowing accessibility for maintenance.

Piston seals, check valves and other parts can be replaced in a matter of minutes.

The 306 model functions as a slave unit, controlled by either a 305 or Trilution® LC Software.

Product #s: 360261 (305 Pump) 3602661 (right) (306 Pump) 3602671 (left) (307 Pump)

402 Syringe Pumps The 402 Syringe Pumps assure accuracy in sample transfers, dilutions, reagent additions, mixing and more. They offer speed and reliability for repetitive liquid handling tasks.

HPLC » Pumps

30X Series HPLC Pumps

Unmatched flexibility and performance for any liquid transfer application - The Minipuls Evolution® is designed for scientists seeking a high performance, manageable peristaltic pump capable of a variety of configurations.

ptimized six roller drive O limits pulsation.

Gilson offers the only peristaltic pump that allows up to 16 separate channel cartridges to be changed while the pump is running with a simple push on the cartridge for placement and removal.

Specifications Tubing ID standard: 0.25 – 4 mm high-flow: 2 – 8 mm Flow Rate standard: 0.2 µL/min – 70 mL/min (42 psi, 3 bar) high-flow: 1 – 340 mL/min (29 psi, 2 bar) Head Speed continuous adjustment from 0 – 60 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm Dimensions (w x d x h) Module: 164 x 182 x 169 mm MF1 pump head: 164 x 56 x 169 mm MF4 pump head: 164 x 92 x 169 mm HF2 pump head: 164 x 92 x 169 mm Reproducibility ≤ 0.1% on all channels

Product #: F110701 (Minipuls Evolution® Pump)

Minipuls 3 Peristaltic Pump Available in three configurations:

402 Single, ideal for sampling injectors.

402 Dual with Tee, provides rapid dilutions in both small and large volumes.

Legendary robustness and ease of integration The Gilson Minipuls 3 multi-channel peristaltic pump is simple to integrate onto existing systems with control through an incorporated keypad or remotely with a separate device.

402 Dual, two-in-one accommodates simultaneous dispensing of multiple reagents and solvents.

Specifications Flow Rate standard: 0.3 μL/min – 30 mL/min (72 psi, 5 bar) high-flow: 1 – 220 mL/min (42 psi, 3 bar) Tubing ID standard: 0.25 – 4 mm high-flow: 2 – 8 mm

Head Speed continuous adjustment from 0 – 48 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm Dimensions (w x d x h) 15 x 17.5 x 18 cm (5.9 x 6.9 x 7.1 in)

The Minipuls 3 accommodates wide flow rate ranges using either the standard or high-flow pump head. Pump heads are available with one, two, four or eight channels.

n ideal addition to Gilson liquid handlers and injectors A including the GX and 215 Series instruments.

Multiple stainless steel rollers drive the pump to produce smoother, low pulse flows.

Specifications Reproducibility 0.8% (10 μL) Accuracy 98.2% (10 μL) Product #s: F410511 (left) (402 Single Syringe Pump) F410512 (right) (402 Dual with Tee Syringe Pump) F410513 (402 Dual Syringe Pump)

23

Automated Liquid Handling | Product Guide

Flow Rate 1 μL/min – 120 mL/min Maximum Pressure 0.8 MPa (8 bar, 110 psi) for 0.1-to 10-mL syringes 0.3 MPa (3 bar, 40 psi) for 25-mL syringe Dimensions (w x d x h) 17 x 20 x 24 cm (6.7 x 7.9 x 9.4 in)

Enhanced reliability and ease of operation are possible because the pump’s speed stability is maintained independent of normal temperature variations from 0˚ to 40˚C. This makes the pump ideal for biological perfusion applications. (Note: not intended for direct connection to human subjects.)

Product #: F155004 (Minipuls 3 Pump, single channel)

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24

171/172 Diode Array Detectors

Versatile, Rugged & Accurate Detection Solutions Gilson offers a complete line of HPLC detection solutions that are ideal for analytical, semi-preparative and preparative HPLC applications.

Quick Comparison Chart Diode Array (DAD)

171/172 Diode Array Detectors

Ultraviolet (UV)

Visible (VIS)

Evaporative Light Scattering (ELS)

Mass Spec.

(MS)

Range

190 – 720 or 190 – 610 nm



Gilson 171 and 172 Diode Array Detectors deliver rugged and accurate detection for both preparative and analytical HPLC. Detection is done with a fiber optic light path that can be configured in a fixed or remote flow cell design.

 

112 UV Detector



MS Detector

An interchangeable flow cell design allows for both the 171 and 172 Detectors to accommodate analytical and semi-preparative to preparative applications.

Specifications Pixels S/N Ratio 171: 256 171: < 3 x 10-4 AU 172: 512 172: < 2 x 10-4 AU Wavelength Pixel Distance 171: 190 – 720 nm 171: ~2.2 nm/pixel 172: 190 – 610 nm 172: ~0.8 nm/pixel Wavelength Accuracy Wavelength Repeatability < 0.5 nm < 0.1 nm Baseline Drift Wavelength Temperature Drift 5 x 10-4 AU/h < 0.005 nm/°C Dimensions (w x d x h) 26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in)

Spectral Band Page Width Channels #

—

8

26

190 – 700 nm

254 or 280 nm

9 nm

0.2 or 12 nm

dual & single

single

26

15X Series UV/VIS Detectors 27

Gilson 151, 152, 155 and 156 UV/VIS Detectors deliver versatile, rugged detection with an interchangeable flow cell design to accommodate analytical to preparative HPLC applications. (see page 72 for Flow Cell Selection Chart)

Detector

ith a remote flow cell, the power of the detector is located directly at W the outlet of the column, which yields virtually no peak broadening.

Product #s: 18100001 (171 Diode Array Detector) 18100002 (172 Diode Array Detector)

15X Series UV/VIS Detectors

prepELS™ II

HPLC » Detectors

HPLC Detectors



—

—

single

27

The following modes are available to specify detection conditions and basic operating parameters: Single wavelength with dual sensitivities (151 and 152) Dual wavelengths (155 and 156) Scan wavelengths (155 and 156) hoose either the Gilson Trilution® LC Software or the stand-alone keypad C control on the 151 and 155 Detectors.

Up to ten methods can be stored.



50 – 3,000 amu

—

7

Specifications

6

Lamp UV: deuterium, 750 hrs VIS: tungsten/halogen, 500 hrs

Setting Accuracy ± 2 nm

Wavelength 190 – 700 nm

Sensitivity 0.001 – 2 AU

Setting Precision ± 0.2 nm

Drift 3 x 10 - 4 AU/hr

Spectral Band Width 9 nm

Dimensions (w x d x h) 26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in)

Product #s: 10105311 (right) (151 UV/VIS Detector, with flow cell/accessory kit) 10105411 (152 UV/VIS Detector, with flow cell/accessory kit) 10105511 (155 UV/VIS Detector, with flow cell/accessory kit) 10105611 (left) (156 UV/VIS Detector, with flow cell/accessory kit)

25

Automated Liquid Handling | Product Guide

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26

HPLC Fraction Collectors

112 UV Detector The 112 UV Detector is a rugged, economical detector for analytical, preparative HPLC and GPC clean-up.

Accessible lamp/filter assemblies for selecting wavelengths.

Fixed wavelength design for increased sensitivity and durability.

0 mm path length analytical flow cell provides excellent sensitivity ranging 1 from 0.001 to 1 AUFS (Absorbance Units, Full Scale).

A front panel dial allows selection of sensitivity level to adjust the absorbency of a sample.

Reduced operating and setup time with a single step baseline function.

E vent-marking function conveniently marks injection point or fraction collector advance on either the recorder or data system.

Ideal Solutions for Small to Large Capacity Collection Gilson offers advanced solutions for fraction collection with a complete line of stand-alone and software-controlled fraction collectors ranging from small footprint to large vessel capacity models and everything in between.

Specifications Lamp mercury (standard): 254 nm, 2,000 hrs phosphor-coated mercury: 280 nm, 500 hrs Spectral Band Width Flow Cell Sensitivity 254 nm: 0.2 < 4 x 10 – 4 AU for flow step 280 nm: 12 of 0.5 – 2 mL/min (Methanol) Drift Dimensions (w x d x h) Wavelength 3 x 10 – 4 32 x 32 x 13 cm 254 or 280 nm AU/hr (12.6 x 12.6 x 5.2 in)

Quick Comparison Chart

Capacity Large

prepFC™

Fraction Collector

Small



Flow Rate

Microplate

200 mL/min

Tubes

1 L Bottles Page #

 

29

Product #: 0310511 (112 UV Detector, with lamp and 11 μL flow cell/accessory kit)

prepELS™ II Detector

150

100 ELSD 80 UV

125 mVolts

100

60

75

40

50

20

25 0

0

2

4

6



FC 204 Fraction Collector

 

20 mL/min





30

20 or 200 mL/min





30

% Moblie Phase

The Gilson evaporative light scattering (ELS) detector, prepELS™ II, provides capabilities on the analyticalscale with the ability to detect as low as nanograms of analyte while a unique filtering technology removes baseline noise without distressing peak height or shape.

FC 203B Fraction Collector

0

Time (minutes)

T he prepELS™ II Detector is ideal for obtaining the purest fractions with the ability to detect virtually any analytes with or without a chromophore, including compounds that have poor UV absorption such as sugars, carbohydrates, lipids and triglycerides.

Product #s: 18100100 (prepELS™ II Detector, 120V) 18100200 (prepELS II Detector, 240V)

Specifications Light Source 670 nm laser diode, < 5 mW Gas Requirement 65 ± 5 psi Nitrogen or Argon Nebulization Temperature 0˚ – 70˚C

27

Automated Liquid Handling | Product Guide

Evaporative Zone Temperature ambient – 120˚C Liquid Flow Rate 0.2 – 5 mL/min Splitting packages available to accommodate system flow rates up to 75 mL/min Dimensions 25 x 46 x 29 cm (9.8 x 18 x 11.5 in)

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28

Gilson prepFC™ Fraction Collector is a large capacity, compact footprint fraction collector ideal for semi-preparative to preparative HPLC applications. The prepFC features an adjustable tray that accommodates various vessel heights, volumes and sizes. prepFC™ Bed Capacity

Sample Quantity 320 192 176 80 54 54 18 11 8 240 56 432 320 176

Size 13 x 100 mm (9 mL) 16 x 100 mm (12 mL) 18 x 150 mm (25 mL) 25 x 200 mm (70 mL) 28 x 57 mm (25 mL) 30 x 115 mm (50 mL) 48 x 113 mm (125 mL) (500 mL) (1 L) 12 x 32 mm (2 mL) (20 mL) 10 x 75 mm (4 mL) 13 x 300 mm (9 mL) 16 x 150 mm (20 mL)

Vessel

Rack

tubes

(4) Code 343 Racks

tubes

(2) Code 223 Racks

tubes

(4) Code 22 Racks

tubes

(2) Code 239 Racks

scintillation (2) Code 204 Racks vials conical bottom (2) Code 222 Racks tubes bottles

(2) Code 211 Racks

bottles bottle

(1) Code 91 Rack (1) Code 92 Rack

vials

FC 203B/FC 204 Fraction Collectors Specifications Dimensions (w x d x h) Valve Dead Volume 50.3 x 39.1 x 34.8 cm 114 μL internal volume; 6 μL dead volume (19.8 x 15.4 x 13.7 in) from common port to normally closed port Liquid Contact Material Flow Rate PTFE and 316 stainless steel 200 mL/min Valve Switching Speed Collection Modes < 20 ms slope, level, time or volume

tubes

(4) Code 341 Racks

tubes

(4) Code 343 Racks

tubes

(4) Code 345 Racks

Choose the compact FC 203B Fraction Collector for small capacity collection or the FC 204 for larger volumes. Collect fractions by time, drop or peak, accommodating all LC applications.

Both models accept a wide variety of collection vessels including tubes, vials and microplates.

In peak mode, collectors monitor a detector signal and identify peaks using either an adaptive-slope algorithm or a specified mV threshold.

T he fraction collector utilizes multi-column adapters for collecting up to 18 separate channels.

prepFC Control Software:

Its compact size fits in most fume hoods.

Remote start and tube advance.

Specifications Dimensions (w x d x h) FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in) FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in)

Valve Switching Speed 100 ms

Max Flow Rate FC 203B: 20 mL/min FC 204: 20 or 200 mL/min Valve Dead Volume FC 203B/FC 204: 64 μL internal volume; 3.5 μL dead volume from common port to normally closed port FC 204: 114 μL internal volume; 6 μL dead volume from common port to normally closed port Liquid Contact Material PTFE and 316 stainless steel

p to ten programmable U collection windows. ctive chromatogram display A with start and stop fraction collection lines.

The 3-way diverter valve prevents contamination of collected fractions (standard on FC 204 and optional on FC 203B).

ustomizable collection patterns C and rack addition.

Up to ten programmable time windows can be added for collecting only samples of interest, while discarding the column’s void volume, areas of non-interest and equilibration volumes.

Contact closure inputs are available to remotely start, advance or stop fraction collection.

High-flow fraction collection valve.

(4) Code 330 Racks

scintillation (4) Code 333 Racks vials

HPLC » Fraction Collectors

prepFC™ Fraction Collector

Increased X/Y speed optimizes fraction collection.

Increase bed capacity by configuring up to four fraction collectors in a single system (provides over 1,000 fractions).

Stand-alone instruments are controlled with an incorporated keypad or through the Gilson Trilution® LC Software.

ocket PC control P for stand-alone operation allows the prepFC™ to be added to any HPLC system capable of sending contact closures.

Product #s: 17110101 (prepFC™ Fraction Collector - Ethernet) 171071 (prepFC™ Fraction Collector)

29

Automated Liquid Handling | Product Guide

Product #s: 171011 (right) (FC 203B) 171011DV (FC 203B, 3-way valve) 171041 (left) (FC 204) 171043 (FC 204, prep valve)

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30

Control Software

Control Software

Trilution® LC Software Gilson has redefined purification with the HPLC system control software, Trilution® LC. This liquid chromatography software, specifically designed for purification, allows for complete control to achieve the challenging results that applications require.

Software Designed by Scientists for Scientists Gilson offers powerful software to complement robust instruments.

With accommodating features, drag-and-drop functionality and numerous supplied tasks, Gilson software packages allow effortless yet complete control of instruments, allowing you to focus on the science rather than the software.

Configuration and Virtual Lab Screen

Quick Comparison Chart HPLC Systems

Trilution® LC

Software

SPE Systems

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GPC Clean-up Systems

Liquid Handlers

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Page #

32 Method Builder Control Screen

Trilution® LH

Software

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33

Fraction Collection Settings Page

Fraction Collection Capabilities:

Application Run Screen

Collection modes: slope, level, time or volume.

Conditional logic fraction collection.

Graphical fraction collection optimization.

Slope collection settings: front, tail and APEX. Auto sample list generation.

Open Access Module: Administrator control and permission-setting capabilities.

Walk-up user access.

Minimal training required.

Conforms to the application needs rather than compromising the application in order to fit the software.

Product #s: 210631R21 (Trilution® LC Media) 21063130 (Trilution® LC Lifetime License) 21063130L5 (Trilution® LC Multi-user Lifetime License - 5) 21063130L10 (Trilution® LC Multi-user Lifetime License - 10) 21063131 (Trilution® LC 1 year License) Note: Requires Oracle® Database 10g Express Edition

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Automated Liquid Handling | Product Guide

Computer Recommendation Processor Intel® Pentium® 4 (2.8 GHz); Intel® Core™ 2 Duo (1.86 GHz)

Fixed Drive 120 GB, available space: 4 GB (6 GB at install)

Video Card 1280 x 1024 with 24 bit True Color

RAM 2 GB

Additional Drive DVD/CD-RW

I/O Ports 1-Serial port 1-Ethernet port

Operating System Microsoft® Windows® XP Professional SP2 or SP3 Intel, Pentium and Core are trademarks of Intel Corporation or its subsidiaries in the United States and other countries. Microsoft and Windows are registered trademarks of the Microsoft Corporation in the United States and/or other countries.

Gilson, Inc. | 800-445-7661 | www.gilson.com

32

GPC Clean-up Systems

Trilution® LH Software Gilson Trilution® LH Software, designed by scientists for scientists, is a complete control solution for liquid handling and solid phase extraction applications. Trilution LH features a user-friendly graphical interface with icon-based drag-anddrop functionality minimizing the learning curve and optimizing application performance.

Ideal Solutions for Your Post-extraction Clean-up Requirements Liquid Handling

Disposable Tips

Solid Phase Extraction

Tweaks

ver 70 Gilson-supplied tasks enable quick O and efficient method programming. uick and effortless instrument setup, Q bed layout and method creation.

S ample list with spreadsheet functionality and graphical run simulation. un methods in either batch or sequential R mode to optimize performance.

Main Menu

anual control for performing M routine tasks such as priming instruments and troubleshooting. raphical custom task creation G and cartridge monitoring.

Auxiliary

The Gilson gel permeation chromatography (GPC) clean-up systems are an ideal solution for post-extraction clean-up of interfering substances from environmental samples, food products and animal tissue, prior to analysis by GC, GC/MS, HPLC or LC/MS. Failure to remove these interfering compounds can cause poor analytical results, shorten column life and increase analytical downtime.

Quick Comparison Chart

Fraction Collection

Multiple Fractions

Solid Phase Extraction

Control

Automated GX-271 GPC Clean-up System

0.25 – 450 mL

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35

(optional)

Trilution® LC Software

GX-271 GPC/SPE Clean-up System

0.25 – 450 mL

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

Trilution® Software

36

307 Pump Keypad

37

Manual GPC Clean-up System

Method in Method Builder Screen

(optional)

0.25 – <1,000 mL



(optional)

Page #

SS Dispense Task Property Page

Computer Recommendation

Gilson GPC Clean-up Systems meet clean-up guidelines set forth by the USEPA, USFDA, USDA, USGS, ISO, CFIA, AOAC International and European Union (EN 1528, EN 12393, S19) requirements.

Fixed Drive Video Card 80 GB, available 1280 x 1024 with space: 4 GB 24 bit True Color (6 GB at install) RAM Additional Drive Serial Ports 1 GB DVD/CD-RW 2 Operating System Microsoft® Windows® XP Professional SP2 or later Microsoft® Windows® 2000 SP4

Processor Intel® Pentium® 4 (2.8 GHz)

Simulation Mode in Application Run Screen

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Automated Liquid Handling | Product Guide

Product #s: 210630R20 (Trilution® LH Media) 21063020 (Trilution® LH Lifetime License) 21063021 (Trilution® LH 1 Year License) Note: Requires Oracle® Database 10g Express Edition

Gilson, Inc. | 800-445-7661 | www.gilson.com

34

GX-271 GPC/SPE Clean-up System

The Automated GX-271 GPC Clean-up System from Gilson is capable of up to 80 sample extractions with large fraction collection capacity into an assortment of vessels.

The Gilson Automated GX-271 GPC/SPE Clean-up System provides SPE capabilities in addition to GPC for sample extract purification; a multi-purpose sample preparation system.

GPC Clean-up Systems

Automated GX-271 GPC Clean-up System

Specifications Automated GPC Systems Compatible with various GPC columns from low pressure glass to high pressure stainless steel.

The GX Direct Injection Module reduces sample waste and carryover, which prevents the need to overfill the sample loop.

Flow Rate 0.01 – 10 mL/min Injection Loop Volume 20 µL – 5 mL Wavelength 254 or 280 nm Maximum Pressure 4,000 psi (276 bar)

Mobile Rack technology enables programmed switching from drain and collect positions to completely automate the entire SPE process from condition to load, wash and elute.

ample Capacity S SPE: 60 (3 mL), 45 (6 mL) GPC: 80

Product #s: 21110000 (Automated GX-271 GPC Clean-up System, with 112 UV Detector) 21110001 (Automated GX-271 GPC Clean-up System)

Automated GPC Systems Bed Capacity Sample Quantity Description GPC Collection Rack 36 38 x 200 mm (170 mL) collection tubes Code 320 Rack 85 25 x 200 mm (70 mL) collection tubes Code 321 Rack 13 61 x 137 mm (250 mL) Boston Round bottles Code 322 Rack 8 Labconco RapidVap (450 mL) flat-bottom evaporation flasks Code 323 Rack 20 Caliper TurboVap II (200 mL) evaporation tubes Code 324 Rack 8 Labconco RapidVap (450 mL) evaporation flasks, with stem Code 325 Rack 50 Caliper TurboVap II (50 mL) evaporation tubes Code 326 Rack 32 Labconco RapidVap (100 mL) flat-bottom evaporation flasks Code 327 Rack 23 49 x 114 mm (125 mL) Boston Round bottles Code 328 Rack 12 Pyrex 4100 (125 mL) flat-bottom flasks for rotary evaporators Code 329 Rack 8 Pyrex (250 mL) flat-bottom flasks for rotary evaporators Code 331 Rack Sample Quantity Description GPC Sample Rack 80 13 x 100 mm (8 mL) tubes Code 343 Rack 44 16 x 100 mm (12 mL) tubes Code 346 Rack

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Automated Liquid Handling | Product Guide

Product # 260440050 260440051 260440052 260440053 260440054 260440055 260440056 260440057 260440058 260440059 260440060 Product # 260440025 260440049

Positive Pressure elution increases reproducibility and precision.

Gilson, Inc. | 800-445-7661 | www.gilson.com

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Solid Phase Extraction Systems

Manual GPC Clean-up System The Gilson Manual GPC Clean-up System is a simple and economical solution within a small footprint requiring minimal bench space.

Specifications Flow Rate 0.01 – 10 mL/min

Wavelength 254 or 280 nm

Maximum Fractions 10 separate

Maximum Pressure 4,000 psi (276 bar)

Injection Loop Volume 20 µL – 5 mL

Whatever Your Requirements for Throughput, Gilson SPE Products Provide a Solution Automate solid phase extraction (SPE) processes with the complete instrument line from Gilson. From 96-well extraction plates to 1, 3 or 6 mL cartridges, Gilson automated SPE solutions meet any application need.

Quick Comparison Chart

Manual injection valve coordinates injection time.

Liquid Handler

Multiple Collection

Positive Pressure Elution

GX-271 ASPEC™

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39

GX-274 ASPEC™

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40

Quad-Z 215 Liquid Handler for SPE

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41

Cartridge Sizes 1 mL 3 mL 6 mL

Page #

Collection of multiple fractions from the same sample extract is done with the VALVEMATE® II Valve Actuator.

Integrate a 112 UV Detector for EPA method compatibility.

Product #s: 21110002 (Manual GPC Clean-up System, with 112 UV Detector) 21110003 (Manual GPC Clean-up System)

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Automated Liquid Handling | Product Guide

ANALYTE

ELUTING

WASHING

LOADING SAMPLE

Keypad provides simple setup and control of the system for time-based fraction collection.

CONDITIONING

A Typical 4-step SPE Method

INTERFERENCES

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38

The GX ASPEC™ Series provides rugged and reliable automation of SPE applications utilizing 1, 3 or 6 mL cartridges.

The GX-271 ASPEC™ is truly the solid solution for low-to mid-throughput sample clean-up and extraction.

utomated A Solid Phase E xtraction Cartridges

GX-274 ASPEC™

Specifications

GX-27X ASPEC™ Series Cartridge Capacity 108 (1 mL), 60 (3 mL), 45 (6 mL) Accuracy 98% (100 μL – 25 mL)

Utilizes Mobile Rack technology to completely automate the SPE process by increasing capacity and flexibility while performing both SPE and liquid handling.

Optional safety shield available.

Solid Phase Extraction Systems

GX-271 ASPEC™

Positive Pressure Modes syringe or gas cylinder/source Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision in comparison to vacuum based instruments.

The GX-274 ASPEC™ is an ideal solution for mid to high-throughput sample clean-up and extraction. obile Rack technology enables the entire SPE M process to be automated from the initial condition to the final elution and transfer while allowing access to cartridges and compounds of interest.

The linear rail system permits automated method development and method optimization in which all or selected steps are collected and evaluated.

Process four SPE cartridges simultaneously for increased throughput.

n-bed transfer ports allow access to two O additional large volume solvent reservoirs; incorporating a VALVEMATE® II provides access to ten off-bed solvents.

On-bed access to four 500 mL or 700 mL reservoirs.

Built-in pressure sensing on each fluid line monitors cartridge pressure and responds to blockage by activating an error handling method.

Choose between the 406 Single or, for more flexibility, the 406 Dual Syringe Pump for both large and small volume capabilities. Both pumps have built-in pressure sensing capabilities for detecting clogged fluid lines and monitoring cartridge pressure on each fluid line.

Product #s: 2614007 (left) (GX-271 ASPEC™, with 406 Single Syringe Pump) 2614008 (right) (GX-271 ASPEC™, with 406 Dual Syringe Pump) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK)

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Automated Liquid Handling | Product Guide

Integrate a GX Direct Injection Module for on-line injection onto HPLC or LC/MS following SPE clean-up.

Positive Pressure elution increases reproducibility and precision due to process consistency.

Product #: 2614010 (GX-274 ASPEC™, with two 406 Dual Syringe Pumps)

Gilson, Inc. | 800-445-7661 | www.gilson.com

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Liquid Handlers

Quad-Z 215 Liquid Handler for SPE Gilson Quad-Z 215 Liquid Handler is a flexible and robust system that performs liquid handling tasks, on-bed filtration and Positive Pressure SPE. This liquid handler accommodates various racks maximizing flexibility for high-throughput applications.

Specifications Cartridge Capacity Accuracy 288 (1 mL), 144 (3 mL), 60 (6 mL) 98% (100 μL – 25 mL) Dimensions (w x d x h) 91 x 61 x 61 cm (36 x 24 x 24 in)

Unmatched Solutions to Meet Your Liquid Handling Requirements Gilson liquid handlers are industry renowned for rugged, reliable performance at an economical price. Whether the application requires a single probe, multiple probes, variable spacing or disposable tips, Gilson liquid handling solutions meet all needs.

Code 300 Series Rack Mobile Rack technology enables automated switching from drain and collect positions to accomplish the entire SPE process from condition to load, wash and elute, including selective evaluation of individual steps for method development and optimization available through N+1 movement into individual collection tubes.

Quick Comparison Chart

Multiple Probes

Disposable Tips Reproducibility

GX-271 Preparative / Analytical Liquid Handler

sing Code 300 Series Racks U with Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision.

Four independent probes with independent variable spacing ranging from 9 to 18 mm for access to various vessel sizes.

GX-274 Liquid Handler



GX-281 Liquid Handler

Quad-Z 215 Liquid Handler



Quad-Z 215 Liquid Handler with Disposable Tips



215 Liquid Handler

223 Sample Changer



Accuracy

Page #

< 1% (100 µL – 25 mL)

98% (50 μL – 25 mL) 98% (10 μL – 5 mL)

43

0.8% (10 μL)

98.2% (10 μL)

45

< 1% (100 μL +)

98% (100 µL – 25 mL)

46

2.1% (1 µL of DMSO)

104% (1 µL of DMSO)

47

4.3% (1 µL of DMSO)

113% (1 µL of DMSO)

47

1.37% (50 µL of DMSO)

99.7% (50 µL of DMSO)

48

0.8% (10 µL)

98.2% (10 µL)

48

Product #: 25101413 (Quad-Z 215 Liquid Handler for SPE )

41

Automated Liquid Handling | Product Guide

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42

The GX-271 Liquid Handler offers a medium capacity with a small footprint. The GX-271 models are economical and robust liquid handlers for reformatting, reagent dispensing and performing serial dilutions. Their modular design allows for future expansion by adding injection and fraction collection capabilities.

Liquid Handlers

GX-271 Analytical Liquid Handler

GX-271 Preparative Liquid Handler Specifications

Specifications

GX-271 Analytical Liquid Handler

GX-271 Preparative Liquid Handler

Accuracy 98% (10 µL – 5 mL)

Accuracy 98% (50 µL – 25 mL)

Flow Rate 1 µL – 5 mL/min

Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

Flow Rate 1 µL – 50 mL/min

Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

(see GX-271 Liquid Handler/Injector page 17)

GX Analytical Solvent System Transfers

GX Prep Solvent System Transfers Liquid Level Detection allows for detecting a variety of solvents as low as 20 µL in a 384-well microplate.

% CV

% CV

Volume

Volume

GX Analytical Solvent System Transfers Volume 1 µL 10 µL 1 mL 5 mL

CV 11.98% 4.5% 0.18% 0.2%

Accuracy ± 300 nL ± 1 µL ± 20 µL ± 100 µL

Consumes only 23" of linear bench space.

F lexible bed accommodates five racks and virtually any tube, vial or microplate.

E thernet based communication provides better connectivity.

Jet Wash Rinse technology provides flowing rinse for fast and efficient rinsing protocols utilizing up to two separate flowing rinse solvents.

GX Prep Solvent System Transfers Volume

CV

Accuracy

50 µL

1.25%

± 2 µL

100 µL 500 µL 1 mL 5 mL 10 mL 25 mL

1.18% 0.48% 0.14% 0.09% 0.07% 0.02%

± 4 µL ± 7 µL ± 5 µL ± 16 µL ± 105 µL ± 46 µL

GX-271 Preparative and Analytical Liquid Handler Bed Capacity Tube Diameter 10 mm 13 mm 15 mm 16 mm 17 mm 18 mm 28 mm

Sample Quantity 540 300 220 220 220 220 70

Note: tubes up to 200 mm can be accommodated

Gilson GX Solvent System, available in an analytical or prep model, features a dynamic range for volumes and flow rates and can access five off-bed solvents.

T he GX-271 Liquid Handler is available without a pump or GX Solvent System, allowing the instrument to be configured with a 402 Syringe Pump.

Product #s: 2614107 (GX-271 Preparative Liquid Handler) 2614108 (GX-271 Analytical Liquid Handler) 2614101 (GX-271 Liquid Handler without pump)

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Specifications Accuracy 98.2% (10 µL)

Reproducibility < 0.8% (10 µL +)

Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

F lexible rack system accommodates tubes, vials and microplates. ptional flowing rinse station with solvent ports O provides active probe rinsing from off-bed solvents.

VALVEMATE® II can be incorporated enabling A access to ten additional off-bed solvents.

The GX-281 Liquid Handler is the premier liquid handling instrument from Gilson. The GX-281 offers unmatched versatility and performance for liquid handling operations including tubeto-plate transfers, serial dilutions, compound reconstitution and sample filtering through the use of Code 300 Series Racks and more. The efficient speed of the GX-281 allows applications to be completed quickly and reliably. The GX Solvent Systems offer a wide range of volumes and flow rates via patented syringeless technology.

20 15 10 5 0

ccess four on-bed solvent reservoirs A without consuming a rack position.

10

25

0

2

4

25 mL Transfers at 25 mL/min.

10 mL Transfers at 25 mL/min.

6 8 10 12 Transfers

Measured Volume

The GX-274 is ideal for bulk reagent dispensing when used in conjunction with a Minipuls Evolution® or Minipuls 3 Peristaltic Pump or for tube-to-plate transfers using 402 Syringe Pumps.

GX-281 Liquid Handler

Volume Delivered

The GX-274 Liquid Handler is a mid-throughput, four probe instrument capable of processing up to four samples (at 18 mm fixed spacing) in parallel, increasing the efficiency of liquid handling applications. The GX-274 Liquid Handler provides an economical option with a small footprint for achieving increased throughput in a variety of applications.

Liquid Handlers

GX-274 Liquid Handler

Specifications Reproducibility < 1% (100 µL +) Dimensions (w x d x h) 87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in)

5 µL Dispense

GX-281 Liquid Handler Bed Capacity

7.5 5 2.5 0

0

20

40

60

Accuracy 98% (100 µL – 25 mL) Flow Rate 1 µL/min – 50 mL/min

80 100 120 140 160 180 Dispenses

Tube Diameter 13 mm 15 mm 16 mm 18 mm 25 mm 28 mm

Sample Quantity 576 576 450 420 180 162

Note: tubes up to 220 mm can be accommodated

Access five off-bed solvent reservoirs for bulk reagent dispensing and multiple solvent rinsing protocols.

Direct request aspirating and dispensing eliminates the time spent waiting for syringes to fill.

Optional integrated bar code scanner verifies rack types and positions.

Product #s: 2614102 (GX-274 Liquid Handler) F410513 (402 Dual Syringe Pump)

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Automated Liquid Handling | Product Guide

Product #s: 261030 (GX-281 Liquid Handler) 261031 (GX-281 Liquid Handler, with bar code scanner)

Gilson, Inc. | 800-445-7661 | www.gilson.com

46

The Quad-Z 215 Liquid Handler was designed based on the robust Gilson 215 Liquid Handler.

This instrument accommodates numerous racks for maximum flexibility of sample transfer between tubes, vials and microplates.

Liquid Handlers

Quad-Z 215 Liquid Handler

215 Liquid Handler Specifications Dimensions (w x d x h) 91 x 61 x 61 cm (36 x 24 x 24 in) Reproducibility Accuracy liquid handling: 2.1% (1 μL of DMSO) 104% (1 μL of DMSO) injection: < 2.0% (500 μL syringe, partial loop); < 5.0% (500 μL syringe, total loop) Injection Carryover Injection Loop Volume < 0.05% 5 μL – 5 mL

The 215 Liquid Handler is a laboratory tested workhorse for performing dilutions, aliquoting solutions, mixing, plate reformatting, reconstituting of samples and more.

The 215 Liquid Handler has a punch strength capable of piercing thick or thin film septa and microplate mats.

Specifications Reproducibility 1.37% (50 µL of DMSO) Carryover < 1 ppb

Accuracy 99.7% (50 µL of DMSO)

Dimensions (w x d x h) 91 x 61 x 56 cm (36 x 24 x 22 in)

Disposable Tips Option Accuracy 113% (1 μL of DMSO)

Reproducibility 0.8% (10 μL +); 4.3% (1 μL of DMSO)

Flexible open bed design allows for a variety of rack options including tubes, vials, microplates and Peltier control.

Integrating two 402 Dual Syringe Pumps provides four independently controlled syringes from 100 μL to 25 mL. For access to the various vessel sizes, the four independent probe variable spacing ranges from 9 to 18 mm.

Product #s: 2510121 (215 Liquid Handler) 2510191 (215 Liquid Handler without pump)

223 Sample Changer The 223 Sample Changer is a programmable sampler for automated sample preparation and transfer.

It provides a cost-effective solution for protocols such as serial dilutions, additions, sampling into vials, timed reactions and tube-to-tube transfers.

The 223 Sample Changer is ideal for automated transfer of samples in analytical techniques like FIA and spectroscopy (UV/VIS, AA and ICP).

Quad-Z 215 Liquid Handler with Disposable Tips

Product #s: 25101413 (Quad-Z Liquid Handler) 2506163 (Quad-Z 215 Adapter Kit for Disposable Tips) 2515154 (849 Multiple Injection Module) F410513 (402 Dual Syringe Pump)

The Gilson Quad-Z 215 Liquid Handler can be configured with a disposable tips option, a requirement in molecular biology and biochemistry applications. Disposable tips prevent crosscontamination, which saves time by eliminating rinsing steps.

This system includes on-bed storage of tips, as well as a chute for collection of used tips.

Specifications Optional flow-through rinse station, available on the left and/or right of the support bar, reduces cross-contamination.

Reproducibility Accuracy 0.8% (10 μL) 98.2% (10 μL) Dimensions (w x d x h) 53.5 x 43.7 x 60 cm (21.1 x 17.2 x 24 in)

Cross-contamination is minimized by adjusting rinse volumes and using liquid level sensing.

hoose between an optional 402 Syringe Pump, C Minipuls Evolution® or Minipuls 3 Peristaltic Pump to add aspirating and dispensing flexibility.

Accommodates a wide variety of probes as well as standard and custom racks.

To see the complete line of Gilson disposable tips see page 57.

Flexible configuration enables use of fixed probes and 10 μL, 200 μL and 1 mL disposable tips.

47

Automated Liquid Handling | Product Guide

Product #: 191015 (223 Sample Changer)

Gilson, Inc. | 800-445-7661 | www.gilson.com

48

VALVEMATE® II Valve Actuator The VALVEMATE® II Valve Actuator automates HPLC and LC techniques by increasing the system flexibility to save time, increase throughput and extend column life. It is designed to facilitate both solvent selection and column switching. The VALVEMATE II increases flexibility of HPLC systems allowing for multiple valve and multiple column configurations.

Optimize Your Instrumentation Complementing robust and flexible instruments, Gilson accessories optimize instrumentation to fit application requirements for the ultimate solution.

High Pressure Valves: Quick Comparison Chart

VALVEMATE® II Valve Actuator

Switching Valve

Mixing

Racks

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Page #

50

Specifications Dimensions (w x d x h) 8.3 x 15.6 x 9.8 cm (3.3 x 6.2 x 3.9 in)

Valve Switching Speed < 180 ms port-to-port (depending on valve)

Low Pressure Valves:

Analytical 2-position, 6-port, stainless steel (0.016" ID ports).

Multi-position, 6-port, PPS (0.030" ID ports).

Analytical 2-position, 10-port, stainless steel (0.016" ID ports).

Multi-position, 6-port, PPS (0.060" ID ports).

Preparative 2-position, 6-port, stainless steel (0.030" ID ports).

Multi-position, 8-port, PPS (0.060" ID ports).

Preparative 2-position, 10-port, stainless steel (0.030" ID ports).

Multi-position, 10-port, PPS (0.060" ID ports).

Preparative 2-position, 6-port, PPS (0.030" ID ports).

Preparative 2-position, 10-port, PPS (0.030" ID ports).

Orbital Shaker

Table of Contents Accessories

Accessories

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51

Multi-position, 6-port, stainless steel (0.016" ID ports).

6 Column Plumbing Schematic

Multi-position, 6-port, stainless steel (0.030" ID ports).

Multi-position, 6-port, PEEK (0.016" ID ports).

RackCount™ System

818 AutoMix

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51

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52

Multi-position, 10-port, stainless steel (0.016" ID ports).

Multi-position, 10-port, PEEK (0.016" ID ports).

Multi-directional keypad control with valve position LED display.

Contact closure control for home and switch valve position.

Fast valve switching times minimize pressure spikes and carryover.

Product #: 331052AB (VALVEMATE® II Valve Actuator)

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Automated Liquid Handling | Product Guide

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The Gilson Orbital Shaker is specifically designed to satisfy liquid handling needs for on-line or off-line vortexing and mixing. It is a fully functional, stand-alone instrument equipped with a single-stepper motor.

Table of Contents Accessories

Orbital Shaker

818 AutoMix Specifications Circular Rotational Speed 20 – 720 rpm Rack Capacity (1) Code 200 Series Rack Dimensions (w x d x h) 11.8 x 31.6 x 4.7 cm (4.65 x 12.44 x 1.85 in)

Designed for Gilson 215 Series instruments, the 818 AutoMix provides fully automated, softwaredriven mixing and sampling. Typical applications that use the AutoMix include mixing whole blood samples, immiscible liquids and more.

Specifications

Mix an entire rack of samples with oscillating mixing.

Mixing Speed/Accuracy 10 rpm: ± 0.5 rpm 20 rpm: ± 1 rpm Rack Capacity (1) Code 200 Series Rack Dimensions (w x d x h) 22.9 x 50.8 x 16.5 cm (9 x 20 x 6.5 in)

T he Orbital Shaker can be placed on the bed of the GX-281 or 215 Series instruments using the optional mounting plate kits.

Product #: 251711 (Orbital Shaker) Product #: 251520 (818 AutoMix)

RackCount™ System

Racks – Standard & Custom

Gilson RackCount™ System, numbered overlays that fit securely on the top of racks, provides a quick and effortless bed location reference for specific samples or collected fractions on a Gilson liquid handler.

Gilson offers standard and custom racks to meet the needs of any and all applications. A variety of vessels can be accommodated including tubes, vials, microplates and microchips.

ackCount™ overlays can be used R with new rack purchases or added to existing Gilson Code 200 Series Racks.

T hey have sequentially numbered fraction collection positions for five complete rack positions and one position for sample locations.

T he numbering patterns are solvent resistant and compatible with most normal and reversed-phase solvents.

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Manual Liquid Handling

Improved Comfort As Simple as pipetman® with Enhanced Ergonomic Features & New Motorized User-friendly Functionality Lightweight and extremely comfortable, the new PIPETMAN® M from Gilson is a fully motorized pipette that requires virtually zero pipetting forces to aspirate and dispense samples. Simply turn the push-button to adjust settings and then click!

Use it like a Mechanical Pipette Keep your technique consistent by using only one button to adjust the volume and to pipette.

PIPETMAN® M is so easy to use that you will have the feeling that you are using a manual pipette.

The standard for accuracy and precision – Motorized

PIPETMAN® M contributes to lowering Repetitive Strain Injuries (RSI): Thanks to its motor, PIPETMAN® M offers you pipetting with minimized pipetting forces.

Low ejection and volume setting forces allow you to work longer without fatigue.

Trusted results Best results guaranteed with a performance exceeding ISO recommendations. PIPETMAN® M helps to avoid variable results due to user inexperience, thanks to motorized pipetting. This special feature guarantees highly reproducible results, especially for small volumes.

PIPETMAN® M Model

Designed with both experienced and inexperienced users in mind, PIPETMAN M pipettes are intuitive and require no learning curve to use.

Motorized piston virtually eliminates pipetting variability among users.

Adjustable piston speed feature allows users to pipette denser samples with accuracy and confidence.

GLP Features

Expanded Flexibility Four essential pipetting modes – reverse, mix, repetitive and pipet.

4,0

Volume Setting Force (N.cm)

Volume range of 0.5 µL to 1,000 µL in only four models.

5,0

Volume (µL)

Systematic Error (µL)

Random Error (µL)

Systematic Error (µL)

Random Error (µL)

F81001

0.5 1 5 10

± 0.04 ± 0.025 ± 0.060 ± 0.080

≤ 0.013 ≤ 0.012 ≤ 0.020 ≤ 0.025

± 0.12 ± 0.12 ± 0.12 ± 0.12

≤ 0.08 ≤ 0.08 ≤ 0.08 ≤ 0.08

F81002

Min :

Max :

2 10 20

± 0.075 ± 0.100 ± 0.150

≤ 0.025 ≤ 0.035 ≤ 0.050

± 0.2 ± 0.2 ± 0.2

≤ 0.1 ≤ 0.1 ≤ 0.1

DF200 DF300

F81003

Min :

Max :

20 100 200

± 0.40 ± 0.80 ± 1.00

≤ 0.15 ≤ 0.22 ≤ 0.26

± 1.6 ± 1.6 ± 1.6

≤ 0.6 ≤ 0.6 ≤ 0.6

DF1000 DF1200

F81004

Min :

Max :

100 500 1,000

± 2.5 ± 3.5 ± 5.0

≤ 0.4 ≤ 0.7 ≤ 1.1

± 8.0 ± 8.0 ± 8.0

≤ 3.0 ≤ 3.0 ≤ 3.0

P10M

DF10 DFL10

P20M

D200 DF30

DF200

P200M

D200 D300

P1000M

D1000 D1200

Service alert reminders.

Product #

Min :

Max :

D10 DL10

Lockable volume.

Color-coded push-button corresponds to matching Tipack™.

PIPETMAN® DIAMOND Tips*

Maximum Permissible Errors Gilson ISO8655

* Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable PIPETMAN DIAMOND Tips is available.

3,0 2,0 1,0

Longer use between charge cycles

0,0

Competitor A

PIPETMAN Neo

Competitor B

Competitor C

PIPETMAN M

IPETMAN M pipettes are available with a calibration certificate P issued by an ISO 17025 accredited laboratory, upon request.

(200 or 300 µL) PIPETMAN M’s Lithium-ion battery has been designed to Model ensure 900 pipetting cycles at maximum speed (up to seven 96 well plates) between charge cycles.

PIPETMAN M charges 80% of its full battery capacity in less than an hour and takes only three hours to fully charge.

Optimized Balance

250 200 Weight (g)

Lightweight, but strong materials and components make PIPETMAN M one of the lightest motorized pipettes ever.

150 100

rA

ito

pet

Com

rB

ito

pet

Com

F E o G D M Ne tor tor tor tor 200 eti eti AN eti eti MP mp mp TM mp mp N E o o o o C C A P C C PI ETM PIP Model Electronic Pipettes (200 µL or 300 µL)

rC

ito

pet

Com

Mechanical Pipettes

Automated Liquid Handling | Product Guide

Safely store and charge your pipette at the same time with the PIPETMAN M Stand Adapter.

Designed to fit in the Gilson Carrousel™ Stand and Single™ pipette holder, the adapter allows you to simplify your daily work and organize your bench.

50 0

53

Complete your equipment with:

Product #: F807023 (Stand Adapter)

Gilson, Inc. | 800-445-7661 | www.gilson.com

54

Manual Liquid Handling

Color-coded push-buttons

PIPETMAN® Neo is an air-displacement pipette that addresses the growing susceptibility of pipette users to repetitive strain injuries.

PIPETMAN Neo exhibits a distinct enhancement in comfort and a wide useful volume-range with the same legendary robustness, accuracy and precision as PIPETMAN Classic.

Wide useful volume range Specifications provided at 10%, 50% and 100% of the nominal volume, as recommended by the ISO8655 standard.

New clip ejector for an easier dismantling and greater tip fitting

lip

c

Six single-channel models cover a range of volumes from 0.2 µL to 1,000 µL.

Helps differentiate PIPETMAN Neo pipettes from PIPETMAN Classic models.

The new clip ejector results in easier daily operation and even more robust system over time.

Up to 50% reduction of pipetting forces Newly engineered springs are wound to provide a significant reduction of pipetting and purge forces.

Up to 50% reduction of pipetting and purge forces on certain models.

45

Dispensing

Pipetting force (N)

40

Expanded flexibility Models P2N and P10N incorporate dual position tip ejectors. Allows use of D10 (short) and DL10 (long) tips – a plus for protection and precision when using microtubes.

Thanks to this improvement, the ejector clips securely in place without risk of it slipping over time.

Purge

35

P200

30

P200N

Trusted results

Short tips

25 20 15

Dual position tip ejector

10 5

Best results guaranteed with a performance exceeding ISO 8655 specifications. PIPETMAN® Neo’s specifications are more stringent than those required to be ISO 8655 compliant.

0 0

5

10

15

20

25

Piston stroke (mm)

PIPETMAN® Neo Models

Long tips

pipetman® Neo Starter Kits

P2N

Enhanced comfort. Maximum savings.

Each Neo Starter Kit contains three PIPETMAN Neo pipettes plus boxes of certified-quality PIPETMAN Tips.

We’ve also included three Jimmy™ microtube openers, three Single™ pipette holders, a “Two Minute Inspection” poster and a Gilson Guide to Pipetting.

PIPETMAN® DIAMOND Tips*

D10 DL10

DF10 DFL10

Model

Product #

Gilson Tips (racks of 96 Tips)

Accessories

PIPETMAN Neo Starter Kit

F167700

P20N P200N P1000N

D200 and D100

3 Jimmy™ microtube openers 3 Single pipette holders 1 Gilson Guide to Pipetting

PIPETMAN Neo PCR Starter Kit

F167600

P10N P100N P1000N

Sterile filter tips DFL10ST, DF100ST and DF1000ST

3 Jimmy™ microtube openers 3 Single pipette holders 1 Gilson Guide to Pipetting

ilson maximum permissible errors are guaranteed only when PIPETMAN pipettes are used with the recommended G Gilson PIPETMAN Tips.

Automated Liquid Handling | Product Guide

F144561

D10 DL10

F144562

P20N

D200 DF30

F144563

P100N

DF100 D200

F144564

PIPETMAN® Neo Starter Kits PIPETMAN Neo Models

Product #

P10N**

Save money when compared to pipettes and accessories purchased separately.

55

eje c to

r

The Pipetting Standard with Lower Spring & Ejection Forces

Color-coded push-buttons facilitate identification of the different models and corresponding quality PIPETMAN® DIAMOND Tips.

P200N

D200 D300

DF200 DF300

F144565

P1000N

D1000 D1200

DF1000 DF12000

F144566

Volume (µL) 0.2 0.5 1 2 1 5 10 2 10 20 10 50 100 20 100 200 100 500 1,000

Gilson Maximum Permissible Errors

ISO 8655 Maximum Permissible Errors

Systematic Error (µL)

Random Error (µL)

Systematic Error (µL)

Random Error (µL)

± 0.024 ± 0.025 ± 0.025 ± 0.030 ± 0.025 ± 0.075 ± 0.100 ± 0.10 ± 0.10 ± 0.20 ± 0.35 ± 0.40 ± 0.80 ± 0.50 ± 0.80 ± 1.60 ± 3.0 ± 4.0 ± 8.0

≤ 0.012 ≤ 0.012 ≤ 0.012 ≤ 0.014 ≤ 0.012 ≤ 0.030 ≤ 0.040 ≤ 0.030 ≤ 0.050 ≤ 0.060 ≤ 0.10 ≤ 0.12 ≤ 0.15 ≤ 0.20 ≤ 0.25 ≤ 0.30 ≤ 0.6 ≤ 1.0 ≤ 1.5

± 0.08 ± 0.08 ± 0.08 ± 0.08 ± 0.12 ± 0.12 ± 0.12 ± 0.2 ± 0.2 ± 0.2 ± 0.8 ± 0.8 ± 0.8 ± 1.6 ± 1.6 ± 1.6 ± 8.0 ± 8.0 ± 8.0

≤ 0.04 ≤ 0.04 ≤ 0.04 ≤ 0.04 ≤ 0.08 ≤ 0.08 ≤ 0.08 ≤ 0.1 ≤ 0.1 ≤ 0.1 ≤ 0.3 ≤ 0.3 ≤ 0.3 ≤ 0.6 ≤ 0.6 ≤ 0.6 ≤ 3.0 ≤ 3.0 ≤ 3.0

* Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable PIPETMAN DIAMOND Tips is available. ** P10N model can be used from 0.5 µL.

PIPETMAN® Neo pipettes are available with a calibration certificate issued by an ISO 17025 accredited laboratory, on request.

Gilson, Inc. | 800-445-7661 | www.gilson.com

56

Manual Liquid Handling

Quality Tips for Researchers Who Care About the Integrity of Their Results Did you know…Tips contribute up to 50% to the performance of your pipette!

Ergonomic Tip Fitting PIPETMAN® Tips and the PIPETMAN Tip holders are designed to work in harmony, which means a low tip-mounting force and a perfect seal every time. Easy to mount. Easy to eject.

Naturally hydrophobic and low-retention Some companies add lubricants to make their tips hydrophobic. Gilson PIPETMAN® Tips are made with the highest grade, 100% pure virgin polypropylene, an inert plastic characterized by low retention and naturally hydrophobic properties so no chemicals leach into your expensive samples.

Fully traceable from the manufacturer to your bench The Gilson Trident guarantees quality. With identification and batch numbers on all PIPETMAN tip packaging, you can be assured that quality is our number one priority.

Prevent sample and pipette contamination with PIPETMAN® Filter Tips The Gilson high-density polyethylene filter blocks small particles down to 1 µm from entering your pipette, providing an effective barrier against aerosol contamination.

Graduated volume markers Volume graduations make it easy to visually check the volume aspirated.

PIPETMAN® Tips Deliver Maximum Accuracy Tip brands: DL-10 A

B

C

D

E

F

G

H

I

Gilson specifications

0.018

Sterilized tips

PIPETMAN® Filter Tips Provide an Effective Filter Against Aerosols

F ree of detectable amounts of RNase, DNase, DNA, RNA, Proteases and trace metals.

0.014 0.012

No contamination. Free of biological contaminants with an SAL of 10-6.

0.01 0.008

All packs are wrapped in medical device quality plastic that provides a complete shield against contamination.

0.006 0.004 0.002 0 -0.125

-0.1

-0.075

-0.05

-0.025

0

Filter effectiveness (% )

Precision (µl)

0.016

Filter Tip brands: Gilson

100

B

C

D

0.8

0.9

95 90 85 80 75 70 0

0.025

A

0.1

0.2

0.3

0.4

0.5

0.6

0.7

1

Aerosol diameter (µm)

Accuracy (µl) 10 measurements of 1 µL at 21°c using a PIPETMAN P10 with various tip brands.

Packaging Our tips are available in convenient rack packaging in our Tower Pack™ refill system or Tipack™ racked boxes for economic and efficient handling.

A Perfect Point Imperfections in a tip’s geometry can lead to excess droplets being released into your results. Gilson prides itself on its perfect point and smooth surface, ensuring that all of your sample is dispensed every time.

57

Automated Liquid Handling | Product Guide

We also offer bagged bulk quantities in our EcoPack™ and EasyPack™ configurations.

Date of sterilization and red sticker sterilization guarantee!

Available in sterilized or autoclavable packages.

Color-coded packaging for GLP compliance.

Gilson, Inc. | 800-445-7661 | www.gilson.com

58

Manual Liquid Handling

Quality Tips for Quality Pipettes PIPETMAN® Tips

PIPETMAN® Tips (Contd.)

Tip Model

Pipette Models

D10

Tipack™ Gilson Tips: 10 Tipacks of 96 racked tips

M=PIPETMAN M P=PIPETMAN P PxN=PIPETMAN Neo U=PIPETMAN Ultra C=PIPETMAN Concept F=Fixed PIPETMAN

Volume Range Tip Length (mm)

P10M, P2, P10, P2N, P10N U2, U10, C10

0.1 - 10 µL 30 mm

Autoclavable Product Number

F171100

Tower Pack™: refill system for Gilson Tips 10 racks of 96 tips

Tower Pack™: refill system for Gilson Tips 10 racks of 96 tips

EcoPack™

EasyPack™

Empty Racks

-Ray sterilized Product Number

Autoclavable Product Number

-Ray sterilized Product Number

Box of 10,000 tips (10 bags of 1,000) Product Number

Box of 1,000 tips (5 bags of 200 or 1 bag of 1,000) Product Number

Set of 2 For manual loading only. Not compatible with Tower Pack refill system.

Product Number

F171101

F167101

F167201

F161630

F161631 F161630/1

F161791 F161393

DL10 (Longer tip)

P10M, P2, P10, P20, P2N, P10N, P20N, P8x20N, P12x20N U2, U10, U20, U8x20, U12x20, C10, C8x10, C12x10

0.1 - 20 µL 45 mm

F171200

F171201

D200

P20M, P200M, P20 to P200, P8x200, P20N to P200N, P8x20N, P12x20N, P8x200N, P12x200N F2 to F200, U20 to U200, U8x20, U12x20, U8x300, U12x300, C100, C300, C8x100, C12x100, C8x300, C12x300

2 - 200 µL 51 mm

F171300

F171301

P200M, P200, P200N, P8x200N, P12x200N U200, U8x300, U12x300, C300, C8x300, C12x300

20 - 300 µL 51 mm

F171400

P1000M, P1000, P1000N, F250 to F1000, U1000, C1200

100 -1,000 µL 66 mm

F171500

D1200

P1000M, P1000, P1000N, F250 to F1000, U1000, C1200

100 - 1200 µL 84 mm

F161112

F161113

D5000

P5000, U5000, C5000

1 - 5 mL 10.4 cm

F161370 (12 Tipacks of 50 tips)

*

D10 mL

P10 mL, U10 mL, C10 mL

1 - 10 mL 16 cm

F161230 (8 Tipacks of 30 tips)

*

Tip Model

Pipette Models

Volume Range Tip Length (mm)

-Ray sterilized Product Number

DF10ST

P10M, P2, P10, P2N, P10N, U2, U10

0.1 - 10 µL 30 mm

F171103

DFL10ST

P10M, P2, P10, P20, P2N, P10N, P20N, P8x20N, P12x20N, F2 to F20, U2, U10, U20, U8x20, U12x20, C10, C8x10, C12x10

0.1 - 10 µL 45 mm

F171203

DF30ST

P20M, P20, P20N, P8x20N, P12x20N F2 to F20, U20, U8x20, U12x20

2 - 30 µL 51 mm

F171303

DF100ST

P200M, P100, P200, P100N, P200N, P8x200N, P12x200N F25 to F200, U100, C100, C8x100, C12x100

10 - 100 µL 51 mm

F171403

DF200ST

P200M, P200, P200N, P8x200, P8x200N, P12x200N F200, U100, U200, U8x300, U12x300, C300, C8x300, C12x300

20 - 200 µL 51 mm

F171503

DF300ST

P200, P200N, P8x200N, P12x200N F200, U200, U8x300, U12x300, C300, C8x300, C12x300

20 - 300 µL 51 mm

F171603

DF1000ST

P1000M, P1000, P1000N, F250 to F1000, U1000, C1200

100 -1,000 µL 51 mm

F171703

DF1200ST

P1000M, P1000, P1000N F250 to F1000 U1000, C1200

100 - 1200 µL 66 mm

F171803

D300 D1000

F161735 F171501

F167102

F167202

F161450

F161451 F161450/1

F167103

F167203

F161930

F161931 F161930/1

F161391

F167105

F167205

F161730

F161731 F161730/1

F161737

F167104 (7 racks of 96 tips)

F167204 (7 racks of 96 tips)

F161670

F161671 F161670/1

F171502

F161100

F161111

F161104

*

F161571 (3 bags of 334)

F161360 (1 rack)

*

F161210 (4 bags of 50)

F161220 (1 rack)

Part Item Number Universal Reload Box F167100 Required for use of Tower Pack tips.

Must be purchased separately.

*Not applicable to this model.

PIPETMAN Filter Tips

59

Automated Liquid Handling | Product Guide

DF300 & DF1200 PIPETMAN® DIAMOND Tips Specifically designed for PIPETMAN® Concept C300 and C1200, DF300 and DF1200 filter tips also perfectly fit all 200 µL and1,000 µL PIPETMAN models, respectively.

New Tipacks now available!

With more space between the liquid and the filter when used with these models, they prevent any risk of having the liquid coming into contact with the filter while still offering high accuracy and precision.

Product #: F171503 (D200ST)

Gilson, Inc. | 800-445-7661 | www.gilson.com

60

Manual Liquid Handling

The TRACKMAN™ prevents errors, saving you time & money!

Versatile Performance

Guiding You to Success

Duo-plate tracker lights the way to your pipetting so you avoid losing track of where you need to start or where you left off.

Get on track, keep on track, as you pipette flawlessly with the help of your new personal laboratory assistant. TRACKMAN™ simplifies your work, improves your productivity and helps eliminate errors and cross-contamination.

Can be used with both 96 and 384 well microplates and single and multichannel pipettes.

Easily switch from using one size plate to another on the same instrument.

Always Operational Long lasting battery allows cordless operation for up to eight hours.

Portable slim design takes up small footprint on your benchtop.

Contemporary Design Light-weight and ergonomic.

1

Maximum Control 16 programming possibilities for pipetting patterns (rows, columns, etc.), pipette types and plate types.

Use with 0.5, 1.5 and 2.0 mL tubes with the optional tube holder accessory.

Optimal positioning.

2

4

Timer function enables hands-free operation.

Optional foot pedal available for hands free control.

3 Intuitive Software Interface 1 Plate types 1.

(96 or 384 wells or microtubes)

2.

2 Indicator of well or tube position

Product #: F70301 (TRACKMAN™)

3.

3 Pipette types (1, 8 or 12 channels) 4 Timer function or manual 4.

16 Programming Possibilities for a Variety of Applications Different pipetting patterns, (individually through rows, columns or other configurations.), different pipette types and vessel types!

Complete your equipment with: Expand the functionality of your Trackman™ with the versatile microtube–holder to transfer liquids from one microtube to another (0.5 mL, 1.5 mL and 2 mL).

Enjoy hands-free operating and individual speed control, when you equip your Trackman with the Gilson optional foot pedal.

Product #: F70105 (Microtube-holder)

Product #: F70261 (Pedal for TRACKMAN®)

61

Automated Liquid Handling | Product Guide

Gilson, Inc. | 800-445-7661 | www.gilson.com

62

Support for Original Equipment Manufacturers Gilson factory to factory direct channel supports the unique design and production requirements of original equipment instrument and assay manufacturers. This allows the OEM partner to focus on their area of expertise while receiving world class reliability, flexibility, documentation and support from Gilson for the automated liquid handling portion of the system.

Gilson OEM channel provides reliable liquid handling instruments and components that integrate with your OEM product, moving your system quickly and reliably to market or adding automated capabilities to existing manual systems. Gilson provides flexible OEM terms and delivery, software development support and on-going training.

Resources

OEM Information

Gilson Knowledge Center Customer Education & Training

Flexibility Wide array of modular design.

Flexible instrument control designed for 3rd party software.

Extensive mechanical, electronic and software engineering and prototyping capabilities to modify existing products to meet OEM requirements.

The Gilson Knowledge Center assists today’s scientists by offering flexible and innovative education and training courses that focus on operating Gilson products and systems for your applications. Choose from our free web seminars, local workshops, classroom training and web training!

Learn more at www.gilson.com

Reliability Stringent specifications and procedures and 50 years of liquid handling experience give reliable, quality solutions.

2011 Customer SPE & Purification Education Program

Long term partnerships are the goals of Gilson.

Quality

Gilson offers many instruments and component to fit your OEM needs. Please contact oem@gilson.com for more information.

Regulatory compliance on standard products.

Extended OEM warranty.

Service, support and validation.

ISO 9001 Certified.

2011 Customer Pipette & Tip Education Program

OEM Instruments & Component Examples

Specifications

OEM GX-Syringe Module Resolution 38,400 steps Precision/Accuracy 0.15 %RSD at 100% stroke length 0.8% accuracy at 500 µL dispense from 500 µL syringe Communication RS-485, up to 32 pumps addressable

Stroke 60 mm Wetted Materials FEP, PTFE, PEEK, Ekonol®, glass, ceramic Valves Selection, multiport valves

Maximum Pressure 0.8 MPa (8 bar, 110 psi) for 0.1-to 10-mL syringes 0.3 MPa (3 bar, 40 psi) for 25-mL syringe

63

Automated Liquid Handling | Product Guide

Specifications

GX-271 OEM Liquid Handler XYZ work zone 389 mm X, 294 mm Y, 200 mm Z

Probe positioning Accuracy: ±0.75 mm in X,Y and Z Repeatability: 0.20 mm in X,Y and Z

Liquid Handling Input/Output External pump connected via Two inputs (contact closure control & calibrated tubing. Gilson syringe, TTL), two relay outputs, two accy ports peristaltic or continuous and reversible (RS-485 and 24v) one safety stop input, flow solvent pumps available.

3-way fraction valve control on Z-arm Communication Options RS-232, RS-485, Ethernet

Punch strength 44 Newtons maximum at 125 mm/sec 53 Newtons maximum at 80 mm/sec

Gilson, Inc. | 800-445-7661 | www.gilson.com

64

Technical Notes Handbooks

Gilson Guide to SPE Automation

Receive ‘how to’ information on the most popular functions in the Gilson Trilution® LH and LC software packages by following step-by-step from our Technical Notes Handbooks. These handbooks guide you through how to perform some of the most important and essential functions that optimize your use of the Gilson Trilution software.

Attend a Gilson quarterly Trilution® G.E.T. LIVE Web Course to be entered for a drawing to receive a complimentary Technical Note Handbook.

Years of experience with Solid Phase Extraction (SPE) and automation of this very important clean-up and sample preparation technique has provided us the opportunity to compile a large amount of tips, best practices, suggestions and optimization/development strategies. Organized into a spiral bound guide, this compiled information is an essential ‘tool book’ for any chemist performing SPE. Inquiries for ordering the Gilson Guide to SPE Automation can be made to your local Gilson Representative, Gilson Distributor or to training@gilson.com.

Gilson HPLC Troubleshooting Guide

Resources

Gilson Publications

Trilution® LH v2.0 and Trilution® LC v2.1 Product #: GKCSPEGUIDE (Gilson Guide to SPE Automation)

Trilution® LH v2.0 Technical Notes Handbook Chapters: Liquid Level Detection & Liquid Level Following.

Virtual Pumping Systems.

Troubleshooting High Pressure Liquid Chromatography (HPLC) systems can be straightforward, despite the multiple components. With our guide that contains helpful forms and checklists, troubleshooting Gilson HPLC systems is simpler than ever! Included in this guide are real-life examples using HPLC systems with real problems and solutions organized into a spiral bound guide to eliminate problems that may be related to software, hardware, column, solvent, or any combination of these. Simple tests to prove purification and reduce or eliminate carryover are included!

Creating Custom Racks.

Resources.

Trilution® LC v2.1 Technical Notes Handbook Chapters: Fraction Collection Fundamentals.

Methods for Verifying Fraction Recovery in Preparative HPLC Systems.

Resources.

Inquiries for ordering the Gilson HPLC Troubleshooting Guide can be made to your local Gilson Representative, Gilson Distributor or to training@gilson.com.

Contact training@gilson.com or visit www.gilson.com to learn more about our Web Courses!

Product #: GKCHPLCGUIDE (HPLC Troubleshooting Guide)

Gilson Guide to Pipetting

Product #: gkctlh21 (Gilson Trilution® LC v2.1 Technical Notes Handbook)

Product #: GKCTLC20 (Gilson Trilution® LH v2.0 Technical Notes Handbook)

How to Order Your Handbook: Inquiries for ordering the Gilson Trilution® LH v2.0 Technical Notes Handbook or Trilution® LC v2.1 Technical Notes Handbook can be made to your local Gilson Representative, Gilson Distributor or to training@gilson.com.

Gilson Quarterly Application Notes Handbooks The complimentary quarterly Gilson Application Notes Handbook allows you to page through scientific advances using Gilson automation and Gilson pipettes in a number of different markets from other chemists and laboratories who have published applications. We welcome you to submit your application to us for publishing in our quarterly handbooks.

Published applications offer everyone a chance to learn about the recent scientific highlights and new technologies. Attend our complimentary G.E.T. LIVE Web Seminars to learn about more scientific advances with industry leading applications and technologies from TODAY! Gilson is the host for our guest speakers, who lead the industry with their innovative methods and techniques. Contact training@gilson.com or visit www.gilson.com to learn more about our Web Seminars - they are FREE!

Get tips and best practices from the experts and inventor of the adjustable volume pipette with our guide to pipetting. Applications, sample solutions and ergonomics are just a few very important topics discussed. This essential laboratory guide compiles years of experience and use of pipettes for different applications in one compact book. Utilize this as a reference guide to assist you with pipetting with the highest degree of accuracy and repeatability in any situation.

Inquiries for ordering the Gilson Guide to Pipetting can be made to your local Gilson Representative, Gilson Distributor or to training@gilson.com.

Product #: LT303409-01 (Gilson Guide to Pipetting)

65

Automated Liquid Handling | Product Guide

Gilson, Inc. | 800-445-7661 | www.gilson.com

66

Food & Beverage Applications

Gilson products support the pharmaceutical, food & beverage, environmental and clinical/forensic markets with a primary focus on purifying samples via preparative HPLC, automated SPE or through GPC clean-up.

Environmental Applications Preservation and Quantification of Hormones in Surface Water Using the Gilson GX-271 ASPEC™ System The contamination of aquatic environments with hormones can disrupt the reproductive and developmental function of aquatic organisms such as fish (Jensen, K.M. et al., 2006; Orlando, E.F. et al., 2004). One source of these hormones is the natural and synthetic hormones that are present in manure from large-scale livestock operations. Approximately 130 billion pounds of manure are produced annually in the United States (USEPA, 2000). The hormones that are present in manure and in crop fields that have been amended with manure can be readily transported to surface and shallow ground water with events such as rain or snowmelt. There is a great deal of interest in studying the distribution and fate of these hormones in surface water. This application note describes the use of different preservatives (sodium azide, hydrochloric acid and sulfuric acid) to inhibit the degradation of hormones in samples of surface water runoff from cattle manure-amended fields during storage at 4° C. The Gilson GX-271 ASPEC™ System was used for this application.

An Automated Method for the Fractionation of Extractable Petroleum Hydrocarbons (EPH) from Water and Soil Extracts

important to determine the types of hydrocarbons that may be present in contaminated soil and water.

Government agencies and other regulatory bodies have developed several methods for determining the types of aliphatic and aromatic hydrocarbons that may be found in contaminated soil or water. The Gilson GX-274 ASPEC was used for this application.

Evaluation of Several Columns and Solvents for Post-Extraction Gel Permeation Chromatography (GPC) Clean-up of Fish Tissue Prior to PCB Analysis Using the Gilson GPC Clean-up System The determination of PCBs, as well as other environmental contaminants, in fish tissue requires extensive sample cleanup prior to analysis by gas chromatography with an electron capture detector (GC-ECD). The high fat content of fish tissue can cause buildup of nonvolatile materials on the GC injection port and the analytical column resulting in poor analytical results and high instrument maintenance costs. Gel Permeation Chromatography (GPC) is a size-exclusion clean-up procedure and it is a highly effective method for the removal of high molecular interferences such as lipids, proteins, pigments and cellular components from animal tissue. The Gilson GPC Clean-up System was used for this application.

GX-274 ASPEC™

Using the Gilson GX-274 ASPEC System Crude and refined petroleum products contain a complex mixture of aliphatic and aromatic hydrocarbons as well as a variety of other compounds.

The concentration of these different hydrocarbons will vary in different products such as crude oil, refined products and other residual oil products.

Many of these hydrocarbons have been shown to pose a risk to human health or to aquatic life.

Leaking underground storage tanks are a common source of groundwater and soil contamination. It is

67

Automated Liquid Handling | Product Guide

Applications

Gilson Applications

GX-271 ASPEC™

GPC Clean-up

An Automated Method for the Selective Solid Phase Extraction of Zearalenone from Wheat Using Molecularly Imprinted Polymers Using the Gilson GX-271 ASPEC™ System Zearalenone [6-(10-hydroxy-6-oxo-trans-1undecenyl)-ß-resorcyclic acid lactone] is a mycotoxin produced as a secondary metabolite of various Fusarium fungi. ZON is also known as RAL, F-2 toxin or ZAR. Zearalenone (ZON) has been detected in a variety of cereal products such as wheat, maize, barley, oats, rice and sorghum as well as beer that has been produced with contaminated grains. ZON can be excreted in cow’s milk after lactating cows are fed ZON in high doses (JECFA, 2000). Exposure to ZON, in animals such as pigs and dairy cattle, has been known to cause estrogenic effects including infertility, reduced serum testosterone levels and sperm counts, reduced incidence of pregnancy and changes in progesterone levels. In addition, ZON can delay the breeding process and cost the producer significant economic and physical losses. Molecularly Imprinted Polymers (MIPs) have been demonstrated to be very effective tools for the selective extraction of an analyte from a complex matrix such as a food product (Haginaka, 2009; Wei et al., 2007). The Gilson GX-271 ASPEC™ System was used for this application.

An Automated Liquid Handling Strategy for Complex Matrices Based on Sample Viscosity Using the Gilson GX-271 ASPEC System Performing manual liquid handling procedures in the laboratory is commonplace. When a laboratory faces an increase in sample volumes and throughput, it is often necessary to automate these liquid handling procedures. Automation of the liquid handling steps should improve day-to-day reproducibility of results. The viscosity of a liquid, defined as the resistance to a fluid to shear motion, can be especially challenging when developing an automated liquid handling protocol.

Liquids with a higher or lower viscosity compared to water may require a different set of parameters to optimize the method. This note describes an automation strategy for the scientist who is transitioning from a manual liquid method to an automated method that is based on the viscosity of the liquid matrix. The Gilson GX-271 ASPEC System was used for this application.

GX-271 ASPEC™

Analysis of Sucralose in Water by Liquid Chromatography/ Time-of-Flight Mass Spectrometry (LC/TOF-MS) after Automated Solid Phase Extraction Using the Gilson GX-271 ASPEC System Non-nutritive sweeteners are steadily increasing in importance with increased awareness of diabetes and its special dietary requirements and with more consumers becoming concerned about obesity and dental caries. Some of the most widely used nonnutritive sweeteners include saccharin, aspartame and sucralose. Sucralose is a water soluble tri-chlorinated disaccharide that is 600 times sweeter than sugar.

Sucralose has a low human toxicity and is not metabolized by the human body. There has been a great deal of interest in monitoring levels of sucralose in water samples (Dye, 2007; Brorström-Lundén, 2007; Mead et al., 2009).Recently, some methods employing liquid chromatography-mass spectrometry (LC/MS) have been published for the analysis of sucralose (Loos et al., 2009) and other artificial sweeteners in water samples (Scheurer et al., 2009). The Gilson GX-271 ASPEC System was used for this application.

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68

Processing Human Serum for Rapid and Reproducible N-Glycan Mass Profiling Using the Gilson GX-274 ASPEC™ System Glycans consist of polymers of monosaccharides joined together by glycosidic linkages. Glycans are complex in structure and can be either branched or linear. Glycans are commonly attached to proteins to form glycoproteins. N-linked glycans (N-glycans) are the most common glycans bound to proteins in eukaryotic cells (Apweiler, R. et al., 1999; Kronewitter, S.R., 2010). N-glycans play a major role in regulating biochemical pathways and in the communication at the cellular level between cells, tissue and organs (Shriver at al., 2004). As such, there is a great deal of interest in the study of glycosylation and N-glycans as biomarkers for a variety of diseases (Lebrilla, C.B., 2009; deLeoz, M.L.A. et al., 2008; Turnbull, J.E. et al., 2007). This application note describes a high throughput sample preparation procedure for global glycan massprofiling. The Gilson GX-271 ASPEC™ System was used for this application.

Identification and Quantification of Illegal Drugs and Benzodiazepines in Human Plasma by LC/MS-MS Analysis after Solid Phase Extraction (SPE)

Pharmaceutical An Automated Method for Dispensing Fruit Fly (Drosophila malonogaster) Food into Culture Vials and Bottles Using the Gilson 215 Liquid Handler The vinegar fly or fruit fly, Drosophila melanogaster, has been utilized in medical and biological research laboratories for over 100 years. It was one of the first organisms used for genetic research and is the genetically best-known of all eukaryotic organisms.

Research in the past twenty years has demonstrated that humans and fruit flies are very similar at the molecular level. About 75% of known human disease genes have a recognizable match in the genetic code of Drosophila melanogaster (Reiter, et al., 2001) and 50% of fly protein sequences have mammalian analogs.

Thus, D. melanogaster is an excellent animal model for studying a variety of human diseases. This application note describes the use of an automated instrument for filling large numbers of culture vials with fly food or fly culture media using the Gilson 215 Liquid Handler.

GX-274 ASPEC™

Using the Gilson GX-271 ASPEC System The identification and quantification of drugs of abuse in blood (plasma, serum, whole blood) has become very common in the forensic medicine laboratory. A variety of methods for the extraction and analysis of these drugs have been described in the literature (Sadeg and Dumontet, 2001; Moeller and Kraemer, 2002), with particular focus on the use of GC/MS or LC/MS for analysis. Liquid/liquid extraction (LLE) has been traditionally used at a basic pH (pH = 9) for the extraction of amphetamines, cocaine and its metabolites and opiates from blood.

This is sometimes followed by an additional cleanup step. This study describes an automated SPE protocol using the Gilson GX-271 ASPEC System.

69

Automated Liquid Handling | Product Guide

Applications

Clinical & Forensic

GX-271 ASPEC™

Automated Solid Phase Extraction of Allantoin from Cosmetics and Topical Pharmaceuticals Prior to Analysis by HPLC Using the Gilson GX-271 ASPEC™ System Allantoin is a heterocyclic organic compound derived from purine. It is also referred to as Glyoxylic Acid Diureide or 5-Ureidhyantoin. Allantoin is a metabolic end product of purine degradation in mammals (with the exception of humans and higher apes), as well as a metabolic intermediate in plants and some bacteria (Young et al., 1944; Fujiwara et al., 1995). Allantoin can be found in many plant species.

Comfrey (Symphytum officinale) has particularly high levels of allantoin. Allantoin has a long history of use in a variety of topical pharmaceuticals and cosmetics for skin care. It has keratolytic, moisturizing, soothing and anti-irritant properties.

Allantoin promotes the renewal of epidermal cells and accelerates wound healing (AKEMA, 2008). Allantoin is used in pharmaceuticals and dermatologic products in the treatment of ulcers, slow-healing wounds, burns, psoriasis and dry skin.

There is a great deal of interest from pharmaceutical and cosmetic manufacturers in determining the amount of allantoin in a variety of products. This application note describes an automated SPE method using the Gilson GX-271 ASPEC™ System for the extraction of allantoin from a cosmetic product.

The Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Dietary Supplements

heart. Ephedrine has a chemical structure similar to amphetamines, differing only in that ephedrine is less potent. The purpose of this application is to present the advantages of using an automated solid phase extraction method coupled with HPLC to determine the various levels of ephedra and ephedra-like compounds in dietary supplements versus manual solidphase extraction. The Gilson 215 Analytical to Semipreparative HPLC System was used for this application.

Automated Fractionation System that Increases Recovery and Optimize Precision of Solid Phase Extraction Methodology Using the Gilson GX-271 ASPEC System Solid Phase Extraction has become a technique of choice for sample cleanup and trace Enrichment. Some of the benefits of SPE are: Versatility, Selectivity, Speed and Low Solvent usage. There are other methods for sample cleanup, such as Liquid-Liquid Extraction, Protein Precipitation, Turbulent Flow and Dialysis.

Most automated methods find their roots in a manual method, but most manual methods don’t optimize the extraction mechanism due to the so called “time” believed to be involved in method development.

Automation in its most general term removes the repetitive/monotonous nature associated with method development and the drain on human capital, while increasing recovery and optimizing precision. The Gilson GX-271 ASPEC System was used for this application.

GX-271 ASPEC™

Using the Gilson 215 Analytical to Semi-preparative HPLC System

215 Liquid Handler

Nutraceuticals or “phytoceuticals” can be defined as natural products that are used to supplement the diet by increasing the total dietary intake of important nutrients. This definition includes nutritional supplements such as vitamins, minerals, herbal extracts, antioxidants, amino acids and proteins. Ephedra is a naturally-occurring substance derived from the Chinese herbal Ma Huang. Its principle active ingredient is ephedrine, which, when chemically synthesized, is regulated as a drug.

The FDA’s concerns about dietary supplements containing ephedra arise in part from ephedra’s mechanism of action in the body. Ephedra is an adrenaline-like stimulant that can have potentially dangerous effects on the nervous system and

215 Analytical to Semi-prep HPLC System

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70

Periodic Table of the Elements

Probes Gilson offers many different types of probes, probe tips and coatings to meet any and all application needs providing solutions for all liquid handling requirements.

Tip Design Flat

Description The tip is cut perpendicular to the length of the probe.

Features Aspirates the last drop of liquid Does not pierce septum No injection

Constricted

The tip of the probe has a smaller outer diameter than the rest of the shaft. Constrictedtip probes accommodate unique tip designs optimizing every application.

Compatible with injection ports

1A

8A

1 H 1.008

2 He 4.003

3 Li 6.941 11 Na 23.00 19 K 39.10 37 Rb 85.47 55 Cs 132.9 87 Fr (223)

2A

3A

The tip is cut at an angle, leaving a bevel.

Sharp tip enables septum-piercing

**Actinides

Compatible with injection ports

58 59 60 57 Ce Pr Nd La 138.9 140.1 140.9 144.2

Low volume, touch-off dispensing.

Two styles:

Sharp-tip enables septum-piercing

1. A shallow groove cut in the probe shaft, above the tip (shown).

ent allows pressures inside and outside V a sealed vessel to equilibrate as liquid is dispensed or aspirated

2. T wo probes are assembled concentrically, creating two separate channels, the inner channel for liquid and the outer channel for gas.

The liquid flow path opens on the side of the probe, not at the tip.

7A

8 O 16.00 16 S 32.06 34 Se 78.96 52 Te 127.6 84 Po (209) 116 Uuh (291)

9 F 19.00 17 Cl 35.45 35 Br 79.90 53 I 126.9 85 At (210) 117 Uus Not Yet Observed

10 Ne 20.18 18 Ar 39.95 36 Kr 83.80 54 Xe 131.3 86 Rn (222) 118 Uuo (294)

90 91 92 93 94 89 Th Pa U Np Pu Ac 227.0 232.0 231.0 238.0 237.0 (244)

95 Am (243)

96 Cm (247)

97 Bk (247)

98 Cf (251)

99 Es (252)

Synthetic

100 Fm (257)

101 Md (258)

102 No (259)

From Decay

103 Lr (260)

Primordial

Flow Cell Selection Chart for Optimizing Sensitivity on 15X Series Detectors Selecting the correct flow cell will optimize sensitivity and peak shape. The following chart pairs flow cells with the appropriate column, flow rate and sample concentration to help choose the correct flow cell for the application.

Capillary Flow Cell

Micro-bore Flow Cell

Analytical Flow Cell

Semi-preparative Flow Cell

Preparative Flow Cell

Product #

104446

104442

104445

104441

104443

Internal Volume

35 nL

1.5 µL

12 µL

0.7 µL

0.16 µL

Recommended for thick septa Prevents coring of septa

Path length

8 mm

2 mm

5 mm

0.2 mm

0.05 mm

Compatible with injection ports

Column Size

180 – 300 µm

1 – 2 mm

4 mm

20 mm

> 40 mm

1 – 15 mL/min

15 – 50 mL/min

30 – 200 mL/min

Purify µg - 5 mg

Purify 5 – 300 mg

Purify 300 mg - 15 g

Vented probes may have different tip designs.

Side-entry

6A

7 N 14.01 15 P 30.97 33 As 74.92 51 Sb 121.8 83 Bi 209.0 115 Uup (288)

Ideal for MALDI spotting applications S pring loaded probe removes any potential of damaging spotting surface

Vented

5A

61 62 63 64 65 66 67 68 69 70 71 Pm Sm Eu Gd Tb Dy Ho Er Tm Yb Lu (145) 150.4 152.0 157.3 158.9 162.5 164.9 167.3 168.9 173.0 175.0

Undiscovered

Spring Loaded

4A

4 5 6 Be B C 9.012 10.81 12.01 12 13 14 Mg Al Si 4B 5B 6B 7B 8B 1B 2B 26.98 28.09 24.31 3B 24 25 26 27 20 21 22 23 28 29 30 31 32 Cr Mn Fe Co Ca Sc Ti V Ni Cu Zn Ga Ge 40.08 44.96 47.90 50.94 52.00 54.94 55.85 58.93 58.70 63.55 65.38 69.72 72.59 42 43 44 45 38 39 40 41 46 47 48 49 50 Mo Tc Ru Rh Sr Y Zr Nb Pd Ag Cd In Sn 87.62 88.91 91.22 92.91 95.94 (98) 101.1 102.9 106.4 107.9 112.4 114.8 118.7 74 75 76 77 56 72 73 78 79 80 81 82 W Re Os Ir Ba Hf Ta Pt Au Hg Tl Pb * 137.3 178.5 180.9 183.9 186.2 190.2 192.2 195.1 197.0 200.6 204.4 207.2 88 104 105 106 107 108 109 110 111 112 113 114 Ra Rf Ha Unh Uns Hs Une Ds Rg Uub Uut Uuq ** 226.0 (261) (262) (263) (262) (277) (276) (281) (280) (285) (284) (289)

*Lanthanides Beveled

References

References

Not recommended with limited volume Deflected

Conical

Based on the beveled tip design, the probe tip is curved and very sharp.

Sharp-tip enables septum-piercing

Designed for PEEK injection ports.

Capable of piercing thin septa

T ip pushes septa out of the way as it pierces to prevent coring

Aspirates the last drop of liquid

71

Automated Liquid Handling | Product Guide

10 mm

Flow Rate

1 – 10 µL/min

25 – 200 µL/min

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72

pH of Common Acids

pH of Common Bases

Acids (pH < 7)

Molarity

pH

Bases (pH > 7)

Molarity

pH

Acetic

1N

2.4

Ammonia

1N

11.6

Acetic

0.1 N

2.9

Ammonia

0.1 N

11.1

Acetic

0.01 N

3.4

Ammonia

0.01 N

10.6

Alum

0.1 N

3.2

Barbital Sodium

0.1 N

9.4

Arsenious

Saturated

5

Borax

0.01 N

9.2

Benzoic

0.1 N

3

Calcium Carbonate

Saturated

9.4

Boric

0.1 N

5.3

Calcium Hydroxide

Saturated

12.4

Carbonic

Saturated

3.8

Ferrous Hydroxide

Saturated

9.5

Citric

0.1 N

2.1

Lime

Saturated

Operating at a wavelength near or below a mobile phase solvent’s maximum UV absorbency increases baseline noise due to the UV response of the solvent.

Refer to the tables below to optimize chromatographic conditions.

Table 1

Table 2

Solvent

Maximum UV Absorbance (nm)

Mobile Phase Conditioners & Buffers

Maximum UV Absorbance (nm)

1-Nitropropane

380

2-Butoxyethanol

220

Acetic Acid, 1%

230

Acetone

330

Ammonium Acetate, 10 mM

205

Acetonitrile

190

12.4

Amyl Alcohol

210

Ammonium Bicarbonate, 10 mM

190

Diammonium Phosphate, 50 mM

205

EDTA, Disodium, 1 mM

190

HEPES, 10 mM, pH 7.6

225

Hydrochloric acid, 0.1%

190

MES, 10 mM, pH 6.0

215

Potassium Phosphate Monobasic, 10 mM Dibasic, 10 mM

190 190

Sodium Acetate, 10 mM

205

Formic

0.1 N

2.3

Magnesia

Saturated

10.5

Amyl Chloride

225

Hydrochloric

1N

0.1

Potassium Acetate

0.1 N

9.7

Benzene

280

Hydrochloric

0.1 N

1

Potassium Bicarbonate

0.1 N

8.2

Butanol-1

215

Hydrochloric

0.01 N

2

Potassium Carbonate

0.1

11.5

Butanol-2

260

Hydrocyanic

0.1 N

5.1

Potassium Cyanide

0.1 N

11

n-Butyl Chloride

220

Hydrogen Sulfide

0.1 N

4.1

Potassium Hydroxide

1N

14

Carbon Disulfide

380

Lactic

0.1 N

2.4

Potassium Hydroxide

0.1 N

13

Carbon Tetrachloride

265

Chloroform

245

Clorobenzene

287

Cyclohexane

200

Cyclopentane

200

Malic

0.1 N

2.2

Potassium Hydroxide

0.01 N

12

Nitric

0.1 N

1

Sodium Acetate

0.1 N

8.9

Orthophosphoric

0.1 N

1.5

Sodium Benzoate

0.1 N

8

Oxalic

0.1 N

1.3

Sodium Bicarbonate

0.1 N

8.4

Succinic

0.1 N

2.7

Diethyl Amine

275

Sodium Carbonate

0.1 N

11.6

Dimethyl Sulfoxide

268

Salicylic

Saturated

2.4

Sodium Hydroxide

1N

14

Dioxane

215

Sodium Chloride, 1M

207

Sodium Citrate, 10 mM

225

Sodium Dodecyl Sulfate

190

Sodium Formate

200

Triethylamine, 1%

235

Trifluoracetic Acid, 0.1%

190

TRIS HCl, 20 mM pH 7.0 pH 8.0

202 212

Sulfuric

1N

0.3

Sodium Hydroxide

0.1 N

13

Ethanol

210

Sulfuric

0.1 N

1.2

Sodium Hydroxide

0.01 N

12

Ethyl Acetate

256

Sulfuric

0.01 N

2.1

Sodium Metasilicate

0.1 N

12.6

Ethyl Ether

220

Sulfurous

0.1 N

1.5

Sodium Sesquicarbonate

0.1 N

10.1

Ethyl Sulfide

290

Tartaric

0.1 N

2

Trisodium Phosphate

0.1 N

12

Ethylene Dichloride

230

Trichloracetic

0.1 N

1.2

Ethylene Glycol

210

Heptane

200

Hexane

195

Isooctane

215

Isopropanol

205

Isopropyl Chloride

225

Isopropyl Ether

220

Methanol

205

Methyl Acetate

260

Methyl Ethyl Ketone

330

Methyl Isobutyl Ketone

334

Methylene Chloride

233

n-Pentane

190

n-Propanol

210

n-Propyl Chloride

225

Nitromethane

380

Petroleum Ether

210

Pyridine

330

Tetrahydrofuran

230

Toluene

285

Xylene

290

Column Sizes & Capacities Internal Diameter (mm)

73

References

Maximum UV Absorbance for Commonly Used Solvents

pH of Acids & Bases

Typical Injection Volume (µL)

Load Capacity (mg)

Typical Flow Rates (mL/min)

4.6

15

1

0.5 – 2

10

100

4.7

4 – 15

21.2

400

19.5

10 – 50

30

1,000

42.5

40 – 100

50

2,000

118

100 – 300

100

10,000

473

400 –1,000

Automated Liquid Handling | Product Guide

Gilson, Inc. | 800-445-7661 | www.gilson.com

74

Solvent Miscibility & Polarity

There are numerous parameters and techniques to optimize preparative HPLC. These reference tables provide important physical parameters of the tubing and injection valve structure; optimization will improve chromatography and percent recovery of collected fractions.

At high flow rates tubing can cause high back pressure.

Ensuring that the appropriate tubing ID/flow rate combination is used will optimize the pumping system.

Solvent Miscibility Table

Table 1: 33X Series pump tubing (PEEK)* Flow Rate (mL/min)

Tubing ID (in) 0.02 Pressure (psi)

0.03 Pressure (psi)

0.04 Pressure (psi)

25

0

0

0

50

43.5

0

0

75

101.5

0

0

100

203.1

14.5

0

125

333.6

43.5

0

150

464.1

87

14.5

175

623.7

101.5

14.5

200

797.7

145

29

*30” lengths of tubing used at corresponding flow rates for tubing pressure. Tubing was connected directly to the pump.

All pre-filters were removed.

Table 2: Rheodyne 7010 – 156 Factory Installed Large-bore Rotor Seal Flow Rate (mL/min)

0.018" make-before-break stator orifice.

1 mL stainless steel 0.030" ID loop. ll other plumbing used is stainless steel A 0.040" ID tubing.

25

Valve Position and Pressure Standard 7010 Bore Size Load Position Pressure (psi)

Inject Position Pressure (psi)

0

14.5

7010 with Large-bore Rotor Gilson Installed Load Position Pressure (psi) 0

Gilson Installed Inject Position Pressure (psi) 0 – 14.5

50

0

43.5

0

43.5

75

0

87

0

116

100

14.5

145

14.5

203.1

150

43.5

290

72.5

435.1

200

87

435.1

145

696.2

Solvent

Polarity Index

Refraction Index @20°C

UV(nm) Cut-off @1AU

Boiling Point (°C)

Solubility Viscosity in water (cPoise) (%w/w)

Acetic Acid Acetone Acetonitrile Benzene n-Butanol Butyl Acetate Carbon Tetrachloride Chloroform Cyclohexane 1,2-Dichloroethane1 Dichloromethane2 Dimethylformamide Dimethyl Sulfoxide3 Dioxane Ethanol Ethyl Acetate Di-Ethyl Ether Heptane Hexane Methanol Methyl-t-Butyl Ether4 Methyl Ethyl Ketone5 Pentane n-Propanol Iso-Propanol6 Di-Iso-Propyl Ether Tetrahydrofuran Toluene Tichloroethylene Water Xylene

6.2 5.1 5.8 2.7 4.0 3.9 1.6 4.1 0.2 3.5 3.1 6.4 7.2 4.8 5.2 4.4 2.8 0.0 0.0 5.1 2.5 4.7 0.0 4.0 3.9 2.2 4.0 2.4 1.0 9.0 2.5

1.372 1.359 1.344 1.501 1.394 1.399 1.466 1.446 1.426 1.444 1.424 1.431 1.478 1.422 1.360 1.372 1.353 1.387 1.375 1.329 1.369 1.379 1.358 1.384 1.377 1.368 1.407 1.496 1.477 1.333 1.500

230 330 190 280 254 215 263 245 200 225 235 268 268 215 210 260 220 200 200 205 210 329 200 210 210 220 215 285 273 200 290

118 56 82 80 125 118 77 61 81 84 41 155 189 101 78 77 35 98 69 65 55 80 36 97 82 68 65 111 87 100 139

1.26 .032 0.37 0.65 0.73 2.98 0.97 0.57 1.00 0.79 0.44 0.92 2.00 1.54 1.20 0.45 0.32 0.39 0.33 0.60 0.27 0.45 0.23 2.27 2.30 0.37 0.55 0.59 0.57 1.00 0.61

100 100 100 0.18 0.43 7.81 0.08 0.815 0.01 0.81 1.6 100 100 100 100 8.7 6.89 0.0003 0.001 100 4.8 24 .004 100 100 100 0.051 0.11 100 0.018

Synonym Table

Immiscible Xylene Water Tichloroethylene Toluene Tetrahydrofuran Di-Iso-Propyl Ether Iso-Propanol6 n-Propanol Pentane Methyl Ethyl Ketone5 Methyl-t-Butyl Ether4 Methanol Hexane Heptane Di-Ethyl Ether Ethyl Acetate Ethanol Dioxane Dimethyl Sulfoxide3 Dimethylformamide Dichloromethane2 1,2-Dichloroethane1 Cyclohexane Chloroform Carbon Tetrachloride Butyl Acetate n-Butanol Benzene Acetonitrile Acetone Acetic Acid

Product #: 25051043 (7010 valve with large-bore rotor seal)

References

Optimizing Preparative HPLC

Miscible Immiscible means that in some proportions two phases will be produced.

1

Ethylene Chloride

2

Methylene Chloride

3

Methyl Sulfoxide

4

tert-Butyl Methyl Ether

5

2-Butanone

6

2-Propanol

Solvent Polarity Chart

Scale-up Factor

(diameter preparative)2 scale-up factor =

(diameter analytical)2 Flow Rate

flow rate preparative

=

scale-up factor

Compound Formula

Group

Representative Solvent Compounds

Non-polar

R-H

Alkanes

Petroleum Ethers, Ligroin, Hexanes

Ar - H

Aromatics

Toluene, Benzene

R-O-R

Ethers

Diethyl Ether

R-X

Alkyl Halides

Tetrachloromethane, Chloroform

R - COOR

Esters

Ethyl Acetate

R - CO - R

Aldehydes and Ketones

Acetone, Methyl Ethyl, Ketone

analytical flow rate

Gradient Duration (GD)

length preparative (diameter preparative)2 flow rate analytical GD preparative = GD analytical x x x

length analytical (diameter analytical)2 flow rate preparative

75

Relative Polarity

Increasing Polarity

Separation Scale-up Equations

Automated Liquid Handling | Product Guide

Polar

R - NH 2

Amines

Pyridine, Triethylamine

R - OH

Alcohols

Methanol, Ethanol, Isopropanol, Butanol

R - COHN 2

Amides

Dimethylformamide

R - COOH

Carboxylic Acids

Ethanoic Acid

H - OH

Water

Water

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76

Common Conversion Tables Fractional inch-to-decimalto-millimeter Conversion Fraction (")

Decimal

Millimeter

1/32

.0313

.7938

1/16

.0625

1.5875

3/32

.0938

2.3813

1/8

3.175

5/32

.1563

3.9688

3/16

.1875

4.7625

Temperature Conversion Formulas

Celsius (°C)

Fahrenheit (°F)

˚C = ˚F – 32 ÷ 1.8

-100

-148

˚C = ˚K – 273.15

˚C = Degrees Celsius

-50

-58

-40

-40

˚F = 1.8 x ˚C + 32

˚F = Degrees Fahrenheit

-30

-22

˚F = ˚R – 459.67

-20

-4

˚K = Degrees Kelvin

-10

14

˚K = ˚C + 273.15

0

32

˚R = Degrees Rankine

5

41

10

50

15

59

˚R = ˚F + 459.67

Pressure Conversion

7/32

.2188

5.5563

20

68

kPa

psi

bar

MPa

1/4

.25

6.35

25

77

690

100

6.9

0.7

9/32

.2813

7.1438

30

86

5/16

.3125

7.9375

35

95

40

104

11/32

.3438

8.7313

45

113

3/8

.375

9.525

50

122

55

13/32

.4063

10.3188

7/16

.4375

11.1125

15/32

.4688

11.9063

1/2

.5

12.7

17/32

.5313

13.4938

9/16

.5625

14.2875

19/32

.5938

15.0813

5/8

.625

15.875

105

221

110

230

21/32

.6563

16.6688

115

239

11/16

.6875

17.4625

120

248

23/32

.7188

18.2563

125

3/4

.75

19.05

25/32

.7813

19.8438

13/16

.8125

20.6375

It is Gilson’s policy to promote a total commitment to quality by each individual throughout the Gilson organization worldwide. It is our goal that this commitment to quality will result in complete customer satisfaction with both Gilson products and services.

ISO 9001 Certification Guarantee of our pipettes’ safety for In Vitro Diagnostics

Gilson is committed to quality, and it shows in everything Gilson does – from design to manufacturing, technical support and training. Due to this commitment to quality and customer satisfaction, Gilson has consistently been awarded with ISO 9001 certification.

Gilson, Inc. and Gilson S.A.S. are both ISO 9001 certified.

34.5

3.4

6890

1000

68.9

6.9

10340

1500

103.4

10.3

131

13790

2000

137.9

13.8

60

140

17240

2500

172.4

17.2

65

149

70

158

20680

3000

206.8

20.7

Customer Education Programs

75

167

24130

3500

241.3

24.1

80

176

85

185

27580

4000

275.8

27.6

90

194

31030

4500

310.3

31

95

203

34470

5000

344.7

34.5

100

212

37920

5500

379.2

37.9

41370

6000

413.7

41.4

Gilson offers industry-leading education for our global customers through our web-based courses, complimentary web seminars, local workshops, and classroom training courses. From travel-free education to our hands-on traditional training options, Gilson provides an extensive program to meet the needs of those just getting started and those looking to advance their skills.

44820

6500

448.2

44.8

257

48260

7000

482.6

48.3

130

266

51710

7500

517.1

51.7

135

275

140

284

55160

8000

551.6

55.2

145

293

58610

8500

586.1

58.6

150

302

155

311

62050

9000

620.5

62.1

160

320

65500

9500

655

65.5

165

329

68950

10000

689.5

68.9

170

338

175

347

180

356

21.4313

7/8

.875

22.225

29/32

.9063

23.0188

15/16

.9375

23.8125

31/32

.9688

24.6063

185

365

25.4

190

374

195

383

200

392

Automated Liquid Handling | Product Guide

Our pipettes are designed, manufactured and calibrated in compliance with the specifications and methods of the ISO 8655 standard.

Gilson Quality Policy

500

.8438

1

High quality of conception and calibration of our products

3450

27/32

1

77

.125

Temperature Conversion

All our pipettes comply with the IVD Directive (98/79/ EC) for In Vitro Diagnostics Medical Devices.

Visit www.gilson.com or contact training@gilson.com for our full Pipettes & Tips or SPE & Purification programs.

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PLC 2020

Personal Purification System (See pages 3– 4)

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