Catalogo harvard apparatus 8

your strategic partners in developing physiologically advanced technologies for

Regenerative Medicine from Research to Clinical Applications

Treatment, Preparation & Harvesting Engineering & Modification Culturing & Growth Physiological Assessment & Condition Monitoring

Regenerative Medicine Electrophysiology & Cell Biology Research

Call to receive other catalogs of interest: Behavioral Research

Harvard Apparatus

Pumps

Electroporation & Electrofusion

Molecular Sample Preparation

Table of Contents Regenerative Medicine

Table of Contents

Introduction................................................................................................................................................................................................ 2-5 Overview of Technical Documents................................................................................................................................................................ 6-9 New Product Summary & Phases ................................................................................................................................................................ 10-11 Table of Contents for Phases 1-4 ................................................................................................................................................................ 12 Phase 1: Preparation & Harvesting Overview Introduction ........................................................................................................................................................................................ 13-14 Drug Delivery/Infusion, Nanomite Syringe Pump .................................................................................................................................... 15 Stem Cell Cooled Injection, NanoCool Delivery System ............................................................................................................................ 16 Fast Bolus Injection System .................................................................................................................................................................. 17 Pulsatile Perfusion, Pulsatile Blood Pump .............................................................................................................................................. 18 Electrospinning, Advanced Polymer Mixing Delivery System...................................................................................................................... 19 Cell Culture Perfusion, PHD ULTRA™ Push/Pull Syringe Pump .................................................................................................................. 20 Pressure Controlled Injection Pump ........................................................................................................................................................ 21 Complex Mixture/Dose Delivery, PHD ULTRA™ Gradient System ................................................................................................................ 22 Accessories: Syringe Warmers .............................................................................................................................................................. 23

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Accessories: Microfluidic Valves, Circuits, Chips & Tubing ........................................................................................................................ 24 Ventilators for All Species ...................................................................................................................................................................... 25 Phase 2: Engineering and Modification Overview Introduction ........................................................................................................................................................................................ 26-27 Cellular Gene Delivery Systems .............................................................................................................................................................. 27 • Electroporation, Electrofusion, Cell Injection System, Lipsomat-Liposome Production........................................................................ 27 Phase 2 Examples................................................................................................................................................................................ 28 • Molecular Biology, Sample Preparation ........................................................................................................................................ 28 • Protein-Drug Binding Systems .................................................................................................................................................... 28 Phase 3: Culturing & Growth Overview Introduction ........................................................................................................................................................................................ 29 InBreath 3D Bioreactor for Hollow Organs Bronchus, Trachea & Blood Vessels .......................................................................................... 30-31 LB-2 Lung Bioreactor............................................................................................................................................................................ 32-33 HPC-3, Hydrostatic Perfusion Chamber for Organ and Tissue .................................................................................................................. 34 Cell and Tissue Perfusion, Imaging & Assay Chambers ............................................................................................................................ 35 Toxin and Chemical Compound Removal, FLOW-Thru Dialyzer .................................................................................................................. 36 Phase 4: Physiological Assessment & Condition Monitoring Overview Introduction ........................................................................................................................................................................................ 37-38 Blood Gas and Ion Measurements, pH, CO2, O2, & Glucose Probes .......................................................................................................... 39-40 Guide for Advanced Metabolic and Physiological Evaluation of Organisms, Organs, Tissues & Cells using IR Contrast Imaging (IR-CI) ............ 41-43 Visible Light Imaging, Digital Magnification System.................................................................................................................................. 44 References ................................................................................................................................................................................................ 45-46 Contact Us.................................................................................................................................................................................................. 47 Note: For Research Use Only. Not for use in humans unless proper investigational device regulations have been followed.

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phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Bioscience companies have partnered with leading global scientists to provide specialized solutions for 110 years… and we are doing it again! researchers across many disciplines to produce clinical and research equipment with…

– the highest performance – unmatched quality – expert support ...necessary to meet the demands of cutting-edge life science techniques.

In keeping with this tradition, we look forward to working with you to develop and supply the next generation of tools to solve the new challenges of Regenerative Medicine from the research lab bench to the patient.

Introduction

Harvard Apparatus and the Harvard Bioscience family of companies are uniquely positioned to develop advanced instrumentation to accelerate Tissue Engineering and Cell Therapy treatments in Regenerative Medicine. From origins in 1901, Harvard Bioscience Companies have worked closely with leading global

Harvard Apparatus Provides Equipment and Expertise for All Phases of Regenerative Medicine Applications PHASE 1

PHASE 2 ENGINEERING & MODIFICATION

Surgical preparation, anesthesia and ventilation, surgical procedures, food and drug delivery, animal and tissue handling, processing and storage.

Gene delivery and modification, cell growth, differentiation and manipulation.

TRANSITION ANIMAL ORGANTISSUE-CELL

RESEARCH

HUMAN ORGANTISSUE-CELL

CLINICAL

Equipment & Methodologies

PHASE 4

PHASE 3

PHYSIOLOGICAL ASSESSMENT & CONDITION MONITORING

CULTURING & GROWTH

Validation of growth-phase and end-stage organ/tissue construct, Monitoring of organ development for transplant suitability.

Scaffold preparation, decellularizaton and Recellularization, tissue seeding, scale-up and growth under physiological conditions.

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

PREPARATION & HARVESTING

3

Introduction

Harvard Apparatus provides advanced solutions across the full range of Life Science & Biomedical Research Techniques Utilized in Regenerative Medicine RESEARCH: COMPOUND SCREENING & DRUG DEVELOPMENT - Protein-drug and protein-protein affinity assays - Infusion pumps for drug, anesthesia, or nutritional delivery - Ion channel and ion transport assays, cellular electrophysiology - Automated animal feeding stations - Amino acid analysis

ORGAN FUNCTION MODELING - Vital sign, Blood Gas and Biopotential Monitoring and Analysis - Ex-vivo Physiology Workstations

MOLECULAR SAMPLE PREPARATION - Desalting samples for Mass Spectroscopy analysis of cell signaling molecules - Isolation of nucleic acid from nuclear membranes - Trace enrich cell metabolic waste products for ID and eventual removal Harvard Apparatus | Advanced Solutions for Regenerative Medicine

CELL ENGINEERING AND GENETIC MODIFICATION - Electroporation - Electrofusion - Pneumatic injection - Live cell imaging and manipulation

MICROFLUIDICS - Syringe Pumps with Nanoliter Precision - Pulsatile Pumps for Physiological Simulation - Microelectromechanical systems (MEMS) - Lab-on-a-chip (LOC) - Micro Total Analysis Systems (µTAS)

TISSUE HARVESTING AND SURGICAL PROCEDURES - Surgical instruments; operating tables and accessories

SAFETY PHARMACOLOGY - Isolated organ & tissue perfusion - Controlled environments for microscope-based screening assays - Electrophysiological Monitoring - Telemetric Physiology Monitoring - Cardiovascular Pressure and Pressure-Volume Analysis

REGENERATIVE MEDICINE AND TISSUE ENGINEERING - Lung Bioreactor for Rat and Mouse, LB-2 - 3D Bioreactor for Trachea/Bronchus and Blood Vesselsm InBreath - Organ Decellularization and Matrix/Scaffold Recellularization - Cell Therapy Injector, NanoCool - Online Physiological and Metabolic Monitoring - Equipment for Organ Harvesting, Transport and Transplant - Anesthesia and Ventilation for Surgery and Recovery

CLINICAL: - 3D Hollow Organ Bioreactor, InBreath

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phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Advanced Solutions for Regenerative Medicine & Tissue Engineering & Cell Therapy Phase 2

Phase 3

Phase 4

Phase 5

Harvard Bioscience Companies

PREPARATION & HARVESTING

ENGINEERING & MODIFICATIONS

CULTURING & GROWTH

CONDITION MONITORING

WEBSITE

Harvard Apparatus

• Identification Products • Ventilators • Anesthesia • Surgical Tools • Infusion Pumps

• • • • • •

• 3D Bioreactors • Toxin & Chemical Removal • Perfusion Systems

• Live Imaging • Blood Gas Measurements • Metabolic Monitoring • Data Aquisition and Analysis • Feedback & Control Syringes

web: www.harvardapparatus.com phone: +1 508-893-8999

Denville Scientific

• • • • • •

• Transfection Reagents • Ligation Kits • Standards • Visualization Kits • DNA Polymerase • PCR

• • • • •

• • • • •

web: www.denvillescientific.com phone: +1 908-757-7577

Hugo Sachs Elektronik

• Ventilators • Operating Tables • Vital Signal Monitors

• Molecular Spectroscopy • Protein Analysis • ds DNA • Oligonucleotide Primers • DNA Melts • Molecular Analysis • Amino Acids Analysis

Warner Instruments

• Cell Imaging

Hoefer

• Molecular Analysis • Cell Membranes • Cytoplasm Toxin Measure • Cell Signaling Chemicals • Blotting

BTX

• Electroporation • Electrofusion • In-vivo Transfection • Pneumatic Injection

Panlab/ Coulbourn

Spinner Flasks Centrifuges Pipetting Systems Incubators Shakers

Protein Measurements ssDNA RNA Phosphorus Enzyme Kinetics

• Isolated Organ Baths • Tissue Baths • 3D Bioreactors

• Electrophysiology • Cardiopulmonary Mechanics • Data Aquisition and Analysis

web: www.hugo-sachs.de/ phone: 0 76 65 - 92 00-0

• Molecular Spectroscopy • Media Monitoring

• Molecular Spectroscopy • Amino Acid Analyzers

web: www.biochrom.co.uk/ phone: +44 (0) 1223 423723

• pH Monitoring • Spill Sensors • Perfusion Chambers

• Electrodes • Amplifiers • Perfusion Chambers • Imaging Chambers • Ion Channel Monitoring • Ussing Chambers • Temperature Control

• Molecular Sample Preparation • Electrophoresis • Affinity • Ion Exchange • SEC/GPC • Filtration • Dialysis

web: www.warneronline.com phone: (203) 776-0664

web: www.hoeferinc.com phone: +1 508-893-8999

web: www.btxonline.com phone: +1 508-893-8999

• Behavioral Apparatus: - Mazes - Rota Rods - Treadmills - Activity Monitors

web: www.panlab.com phone: 34 934 750 697 web: www.coulbourn.com phone: +1 610-395-3771

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Biochrom

Spinner Flasks Centrifuge Pipetting Systems Incubators Shakers Sterilizers

Fast Bolus Injections Cell Delivery Systems Iontophoresis Liposome Prep Drug Delivery Systems Capillary Glass

Introduction

Phase 1

5

Ask about our

Our Harvard

catalogs, technical guides, application notes and bibliographies

We have Solutions for

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Technical Documents

Harvard Apparatus has the largest global support network of experts, and broadest range of specialized products to assist you in finding the best solutions for your experimental research.

For Animal, Organ & Cellular Physiology and Behavioral Research

PHYSIOLOGY

BEHAVIORAL

Equipment for virtually any Animal, Organ or Cell Biology Experiment from animal handling to drug infusion to physiological monitoring.

Fully integrated research systems covering a range of powerful behavioral assays.

For Cell/Tissue Engineering, Imaging and Electrophysiology

MICROSCOPIC LIVE CELL: IMAGING, INJECTION & ELECTROPHYSIOLOGY Amplifiers, imaging, micromanipulation and perfusion for the electrophysiological and neurological sciences.

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TRANSFECTION

CELL MODIFICATION

A comprehensive line of instruments and accessories for both electroporation and electrofusion of mammalian, bacterial, yeast, fungi, insect and plant cells and tissues.

This guide explains Liposomes, Pneumatic Injection, Iontophoresis, Electroporation and Mechanical Injectors.

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Apparatus Family:

all Regenerative Medicine Research Applications Call 508-893-8999 or email regen@harvardapparatus.com for technical support or to request a catalog or Technical Application Guide.

ISOLATED HEART

Features products used specifically for smaller organisms including Drosophila, Nematodes, Xenopus and Zebrafish.

Detailed system guide for the ultimate ex-vivo perfusion system for small rodent heart, the IH-SR.

Specialized Guides for Bioresearch

EPITHELIAL TRANSPORT Discusses the theory of operation and presents the largest collection of Ussing Systems available.

CELLULAR ELECTROPHYSIOLOGY TOOLS Detailed guide for a range of tools for cell based electrophysiology assays.

BILAYER WORKSTATION Focus on the Planar Lipid Bilayer Workstation as a foundation for drug screening as well as ion channel structure and function.

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

MODEL ORGANISMS

Technical Documents

Specialized Tools for Model Organisms and Ex-Vivo Physiology Systems, like the IH-SR for Isolated Heart Perfusion

77

Ask about our

catalogs, technical guides, application notes and bibliographies

Our Harvard

We have Solutions for

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Technical Documents

Harvard Apparatus has the largest global support network of experts, and broadest range of specialized products to assist you in finding the best solutions for your experimental research.

Advanced Organ, Tissue and Cellular Engineering and Therapy Tools for Regenerative Medicine

REGENERATIVE MEDICINE 3D Bioreactors for Lung, Trachea/Bronchus & Blood Vessels.

Stem Cell Injection Systems for Fast Bolus Injection, Pressure-Controlled Fluid Delivery, Active Cooling & Mixing of Cell Suspensions for Injection, Electrospinning.

Smooth High Accuracy Flow, Very High, Very Low or Physiological Flow Rates

NANO & MICROFLUIDICS, PUMPS & INFUSION Pulsatile Blood Pumps, Peristaltic Pumps, Syringe Pumps and full line of connectors, tubing and accessories.

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phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Apparatus Family:

all Regenerative Medicine Research Applications Call 508-893-8999 or email regen@harvardapparatus.com for technical support or to request a catalog or Technical Application Guide.

BIOCHROM: Amino Acid Analyzers, Full Family of Spectrometers BTX: The In Vivo and In Vitro Experts in Electroporation and Electrofusion DENVILLE SCIENTIFIC: A Complete Family or PCR, Incubators and Mixers HOEFER: The Electrophoresis People, Molecular Sample Preparation

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

1D AND 2D ELECTROPHORESIS, WESTERN BLOT, IMAGING SYSTEMS, PROTEIN BINDING SYSTEMS, SPE CLEAN-UP, DIALYSIS CLEAN-UP, PCR REAGENTS, INCUBATORS, MIXERS

Technical Documents

Molecular Biology and Assays, Sample Preparation and Separations

99

New Product Summay

NEW Systems for Regenerative and Tissue Engineering

Our newly developed systems are guided by breakscientists and surgeons to ensure performance, Stem Cell Cooled Injection with high viability, NanoCool Delivery System (p. 16) Developed in the field, specifically to inject 1 ul to 100 ul of stem cells into live tissue while maintaining cell viability in an accurate and easy-to-use system.

The NanoCool cools,cells to 18 degrees in the syringe during injections.

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Now a system to deliver pressure-controlled fluid injection to intact organs and tissues. The system delivers rapid injections while simultaneously monitoring pressure at the injection site. Measured pressure modulates pump speed for tight control of delivered injection pulse.

Complex Automatic Mixture/Gradient Delivery & Dosing System (p. 22) Stepped Flow Rate

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Fast Bolus Injection System for rapid, controlled fluid delivery (p. 17)

Continuous Ramped

The Harvard Apparatus Mixture/Gradient systems allow you to deliver Complex Mixture and Dosing Protocols by controlling multiple flow streams with variable % concentration automatically. These serial dilutions or changes are preprogrammed using easy set-up Pump Methods. Injection can also be initiated by a triggering event from another device, allowing feedback and control loops to be easily implemented. For example, a pH sensor could trigger injection of buffer solution to a mixed drug dose in order to maintain pH at a set level.

Time

InBreathe Hollow Organ 3D Bioreactor, proven through human transplantation (p. 30-31) Clinically proven and published bioreactor design used in successful human transplantation of bronchus. Produced in conjunction with Politecnico Di Milano and University of Barcelona, the InBreath bioreactor is ideally suited to regeneration of tubular hollow organs such as trachea/bronchus, blood vessels, esophagus and intestines. phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Medicine, Cell Therapy

Dedicated Lung Bioreactor with online pulmonary monitoring (p. 32) Proven and published whole organ bioreactor specifically designed in conjunction with Massachusetts General Hospital for generation of rodent lungs.

Real-time monitoring of respiratory mechanics and Active Ventilation during growth phase for organ performance validation and successful transplantation.

New Product Summary

through research in conjunction with leading validity and scientific relevance.

Sterilizable pH, CO2, O2 and GLUCOSE SENSORS (p. 39)

Hand-Held Microscope up to 500X Magnification (p. 59)

Small, light weight digital microscope for Organ and Tissue preparations, microsurgery, injection or cannulation. Remotely placed monitor (or PC Connection) enables simple mounting to bioreactor-based systems.

Harvard Apparatus Partners Program Harvard Apparatus is partnering with global scientific partners in academia and industry to license, co-develop or test device concepts that will accelerate regenerative medicine, cell therapy and tissue engineering in the research and clinical marketplace. This proven development model has consistently resulted in refined, successful and relevant commercial products. For development of advanced, specialized systems for cell, tissue, organ and in-vivo assays please contact us. Contact Ron Sostek 508-893-8999 x 1100, rsostek@harvardapparatus.com phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Blood gas and metabolite measurements are critical determinants of organ and tissue viability and performance. These sensors are available in a range of sizes and configurations as well as bioreactor-integrated forms for online monitoring.

Sterilizable and made of completely inert material, these sensors are ideal for bioreactor use.

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NEW Systems for Advancing Regenerative

Phases

Medicine & Tissue Engineering Phase 1: Treatment, Preparation & Harvesting

Phase 3: Culturing & Tissue Growth

1. Identification Products

1. Cell & Tissue Preparation 2. Cell Growth

- Tattoo - Clips - RFID

2. Hair Removal Products 3. Surgical Equipment - Operating Tables & Lights - Magnification Products

4. Surgical Instruments - Tools - Needles - Sutures - Catheters - Sterilization Equipment

5. Anesthesia Equipment 6. Ventilation Equipment 7. Vital-Sign Monitoring

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

- Capnographs - Homeothermic Blankets - Pulse Oximeters - Blood Pressure - EKG - Others

8. Recovery Chambers 9. Infusion Products - Drug Delivery - Anesthesia Delivery - Nutrient Delivery

Phase 2: Engineering & Modification 1. Cell or Tissue Modification: In Vivo, In Vitro, Gene Delivery - Pneumatic Femto Injectors - Electroporation - Electrofusion - Iontophoresis - Liposomes - Mechanical Injectors

2. Cell or Tissue Accessories - Capillary Tubes - Capillary Pullers - Capillary Forges - Homogenizers - Manipulators

3. Perfusion Chambers - Imaging Culture Plates

4. Molecular Isolation, Purification Analysis - Amino Acid Analysis - Electrophoresis - Western Blot - Protein-Ligand Interactions - Protein-Protein Interactions - DNA purification Kits

- Media Infusion Pumps a. physiological b. continuous flow - Imaging Chambers

3. Cell Sorting - Capillary Glass - Manipulators

4. Cell Harvesting 5. 3D Organ Bioreactors 6. Spinner Flasks 7. Centrifuges 8. Pipetting Systems 9. Tissue Culture 10. Incubators 11. Shakers 12. Continuous flow pumps

Phase 4: Physiological Assessment & Condition Monitoring Cells 1. Microscopic Imaging & Perfusion Chambers 2. Microscopic Environmental Controls 3. Electrophysiology Products 4. Condition Monitors

Tissues 5. Isolated Tissue & Perfusion Baths

Organs 6. Lung Regeneration Bioreactor 7. 3D Bioreactors – Hollow Organs

Sensors 8. Ion Sensors Ammonia, Calcium, Chloride, Ethanol, Glucose, Lactate, Nitrate, Nitric Oxide, Nitrite, Peroxide, Potassium, Sodium, Urea 9. Flow 10. Temperature 11. Pressure 12. Force 13. pH, O2, CO2

5. Molecular Characterization - Spectrometers

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phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

PHASE 1: Treatment, Preparation & Harvesting Preparation & Harvesting of Cells, Tissues & Organs 1. Identification Products - Tattoo - Clips - RFID

2. Hair Removal Products 3. Surgical Equipment - Operating Tables & Lights - Magnification Products

4. Surgical Instruments - Tools - Needles - Sutures - Catheters - Sterilization Equipment

6. Ventilation Equipment 7. Vital-Sign Monitoring - Capnographs - Homeothermic Blankets - Pulse Oximeters - Blood Pressure - EKG - Others

Phase 1: Introduction

Introduction to Phase 1 Tools, Equipment and Systems

8. Recovery Chambers 9. Infusion Products - Drug Delivery - Anesthesia Delivery - Nutrient Delivery

5. Anesthesia Equipment

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Phase 1 Catalog and Guides

13 13

Phase 1: Experiments

PHASE 1:

Treatment, Preparation & Harvesting: An Experimental Example

Prepare animal & inject drugs or stems cells into an organ or tissue to promote regeneration EXAMPLE: CARDIAC INFARCT AND STEM CELL DELIVERY APPLICATION

EXPERIMENTAL GOAL: Stem cells are delivered around a cardiac infract (circled). Four to ten injections of volumes in the 5ul range are delivered. Each injection is delivered over a 3 second period.

INJECT ANESTHESIA: STEP 1: OPEN CHEST CAVITY STEP 2: INITIATE INFARCT STEP 3: INJECT STEM CELLS

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

STEP 4: CLOSE WOUND AND RECOVERY

Step 2, 3 & 4

Step 1

• Mouse Ventilator

• Hair Clipper

• Rat Ventilator

• Stem Cell Delivery Pump

• Micro Vascular Occluder • Homeothermic Blanket

• Hot Bead Sterilizer

• Mouse & Rat ID Tags

• Sugical Tools

• Fiber Optic Illuminator

• Capnograph • Recovery Chamber • Bag Restrainers 14 14

• Magnifying Glasses

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

NEW Nanomite for Limited Volume Drug Delivery Hand Held or Stereotaxic Mounted

Superior volume delivery

Nanomite Figure 1: The blue line above, clearly shows the high accuracy and smooth flow of the Nanomite injection compared to two optimal hand injections.

Flow ramping injections for optimal spatial cell delivery

You need only to target the area of interest and step on the foot pedal.

• Entire flow method recalled and performed • High accuracy and precision cell delivery system from 1.3pl to 100 µl injections with unmatched performance from up to a 1 ml syringe.

• Can be combined with our micro needles down to gauge 37.

• Cells dispensed and next injection ready

Figure 2: Tissue creates backpressure but flow is gently ramped so tissue has a chance to expand to accommodate liquid displacement. You program a volume and time to be delivered, Nanomite does the rest.

Specifications # of Syringes

1

Accuracy

±0.5%

Cable Length

6 ft (1.8 m)

Cable Length

Flow Rate Minimum

1900 µl/min

Injector Head/Actuator

7 x 1-3/8 x 2 in (17.8 x 3.5 x 5.1 x cm) (L x H x W)

Controller Dimensions Flow Rate Maximum

4-1/2 x 9 x 4-1/2 in (11.4 x 22.9 x 11.4 cm) (H x W x D) 3.3 nl/hr

Pump Function Infusion, withdrawal, volume dispense or continuous pumping modes Reproducibility

±0.1%

Syringe Diameter (Max)

6.00 mm

Syringe Size

Features & Benefits

Maximum Minimum Syringe Types

• Light weight makes it ideal for hand-held stereotaxic injection • Easy-to-use LCD color touch screen with GUI interface

0.5 µl 1 ml

1700 , 7000 , 700 and 1000 Hamilton syringes, or any other syringe manufacturer with known ID in mm

Order #

Product

70-3601

Nanomite, Infuse/Withdraw

• Multiple methods storage makes running simple or complex methods easy

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

The Nanomite does all the work.

• High performance in a small package • Better performance than manual injections • Version for automatic infusing and withdrawing • Hand-off injections use foot-pedal activation phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

15 15

NEW NanoCool™ Injector: Fixed 18°C for Optimal

Cell Viability or Drug Delivery NanoCool™

20% increase in survivability, 76% Lower O2 consumption rate at 18 vs. 37 degrees Celcius NanoCool Hand-held or Stereotaxic Mounted Injector

NanoCool Touch Screen Controller

Foot-Pedal

NanoCool™ Cell Delivery System Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• 18˚C syringe and eppendorf

16 16

• Flow ramping to reduce injection site blowback • Programmed methods for automatic recall of entire cell delivery system. Up to 100 methods stored for recall • 2 year warranty. Certified with CE, UL, CB Scheme

Temperature effect on O2 Consumption by Organ Cell Type Organ

Temperature

Oxygen Comsuption

Change in O2 Consumption

% Change

Kidney

37 to 18

3.7 to 0.9

2.8

75.7

Liver

37 to 18

3.0 to 0.6

2.4

80

Heart

37 to 18

2.4 to 0.7

1.7

70.8

Brain

37 to 18

1.9 to 0.4

1.5

78.9

Muscle

37 to 18)

0.8 to 0.2

0.6

75

Skin

37 to 18

0.4 to 0.1

0.3

75

• High accuracy delivery between 1.3 pl/min to 68ml/min • Preprogrammed bolus injection mode, just specify injection size and time of dispense

Figure 1: IR Temperature Image NanoCool at 18°C.

• Foot pedal start keeps hands free When injecting stem cells viability of cells can be a major issue. The Harvard Apparatus NanoCool™ is the only cooled cell injection system with optional cooled and oxygenated vortex mixer. The injection system and sample vortex holder are held at 18°C. The cell delivery is totally integrated in it operation through the NanoCool’s microprocessor system: power, temperature injection volumes and flow ramping are all controlled by the NanoCool’s program. Keeping the cells at 18°C instead of 37°C reduces O2 consumption of cells by an average of 76%. The chart below shows the different organ utilization curves.

Specifications Accuracy

±0.5%

Alarms

End-of Run alarm and over pressure alarm

Average Linear Force

11 to 12 lbs

Syringe Diameter (Max)

6.00 mm

Syringe Size Maximum Minimum

1 ml 0.5 µl

Syringe Types

1700 , 7000 ,700 and 1000 Hamilton syringes, or any other syringe manufacturer with known ID in mm

Injector Head/Actuator

7 x 1-3/8 x 2 in (17.8 x 3.5 x 5.1 x cm) (L x H x W)

Order #

Product

70-3040

NanoCool™ Injector Infusion/Withdrawal Programmable Single Syringe

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Fast Bolus Injector for Cell Therapy Fast Bolus Injector

Figure 1: Bone Marrow Aspiration & infusion Needles

Fast Bolus Injector • Inject a fast bolus of large or small volumes in a second; up to 12X faster than standard pumps

Figure 2: Bone Marrow Injection Sites.

Specifications

• Programmable with multiple methods

Accuracy

±0.50%

Reproducibility

0.5 µl / 140 ml

• Easy-to-use icon interface • CE, ETL, CSA, UL and CB scheme Global compliance • Delivery from picoliter to hundreds of Ml/min For delivery of fast infusion or bolus injections into Liver, Kidney or Bone the Fast Bolus Injector makes it easy. The Fast Bolus Injector can mount a 40 ml syringe or larger and accurately inject 100 nl or 100ul per injection with the push of a foot pedal. This high linear force pump can pump up to 85 to 433 pounds of force, easily tp inject viscous materials cell suspensions or pastes.

Also a full selection of research surgical products are available: Bone aspiration and infusion needles, syringes, surgical equipments, anesthesia circuits, surgical drapes and sponges, ventilators capnographs and pulse oximeters, catheters, sutures, bone drill and more.

Bolus Delivery Programming, it is as easy as setting the amount to be delivered and the time frame you want to deliver i.e 5ul in three seconds.

Human Bone Marrow–Derived Mesenchymal Stem Cells for Intravascular Delivery of Oncolytic Adenovirus 24-RGD to Human Gliomas, [Cancer Res 2009;69 (23):8932–40] .

±0.05%

Syringes (Min./Max.) Flow Rate: Minimum Maximum Display

1.56 pl/min using 0.5 µl syringe

215.8 ml/min using 140 ml syringe 4.3" LCD Color Display with Touchpad Non-Volatile Memory Stores all settings

Connectors: # Syringes/Pump

2,4,8,10 (1.4 Liters capacity)

Linear Force (Max)

34 kg (75 lbs) @ 100% force selection

Pusher Travel Rate: Minimum Maximum

0.18 µm/min

190.80 mm/min

Dimensions

10.16 x 30.48 x 21.59 cm (4 x 8.5 x 12 in) (H x W x D)

Weight

4.5 kg (10 lbs)

Regulatory Certifications CE, UL, CSA, CB Scheme, EU RoHS

Order #

Product

70-3045

Fast Bolus Injector

Multiple methods can be stored and recalled via touch of a button, making the Fast Bolus Injector the ultimate, easy to use syringe pump. Methods can be emailed and downloaded from your PC into the pump. Share a method with a colleague or duplicate a complex experimental set-up with an e-mailed method.

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• The highest accuracy and precision available

Figure 3: Catheter placed into right hepatic vein.

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Pulsatile Blood Pump

Pulsatile Blood Pump for Generation of Physiological Flow

Figure 1: A Novel Culture System Shows that Stem Cells Can be Grown in 3D and Under Physiologic Pulsatile Conditions for Tissue Engineeringof Vascular Grafts,Oscar Abilez, et al Journal of Surgical Research 132, 170–178 (2006) doi:10.1016/j.jss.2006.02.017.

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Pressure and Flow Curves Using Harvard Apparatus Model 1421 Pulsatile Blood Pump in Isolated Perfusion of Left Lower Lobe of Dog Lung

Pulastile Blood Pump • Pulsatile output truly simulates the ventricular action of the heart • Minimal hemolysis • Models for mice to large animals • Ideal for moving emulsions, suspensions, and non-Newtonian fluids such as blood

Figure 2: Pa = Pulmonary, Artery, Pressure; Pv = Pulmonary, Venous, Pressure; Qpa = Pulmonary Artery Blood Flow. Istrumentation: Pressure (Statham), Flow (Blotronex Electromagnetic Flowmeter, Recording (Electronics for Medicine.

It truly simulates the pumping action of the heart. It features silicone rubbercovered heart-type ball valves and smooth flow paths which minimize hemolysis. Only inert materials like silicone rubber, acrylic plastic, and Teflon contact the fluid. The pumping head is easy to take apart and reassemble and can be sterilized.

Note: The above data is supplied through the courtesy of Cardiorespiratory laboratory Columbia-Presbyterian Medical Center New York, New York, Dr. Alfred P. Fishman, Director.

Outstanding Performance

Specifications

The pulsatile output closely simulates the ventricular action of the heart. This action provides physiological advantages in blood flow for perfusion in cardiovascular and hemodynamic studies. It is ideal for isolated organ perfusion, whole body perfusion, blood transfers, hydration/dehydration procedures and blood cellular profile studies.

Pump Mechanism A positive piston actuator and ball check valves provide the proportioning action. The product of stroke rate times stroke volume is an accurate indicator of the flow rate. Positive piston action prevents changes in flow rates, regardless of variations in resistance or back pressure. The piston always travels to the end of the ejection stroke, independent of the volume pumped.

The Pump completely empties at each cycle.

Accuracy

±0.35%

Reproducibility

0.5 µl / 140 ml ±0.05%

Syringes (Min./Max.) Flow Rate: Minimum Maximum Display

1.56 pl/min using 0.5 µl syringe

220.97 ml/min using 140 ml syringe 4.3" LCD Color Display with Touchpad Non-Volatile Memory Stores all settings

Connectors: # Syringes/Pump

2,4,8,10 (1.4 Liters capacity)

Linear Force (Max)

34 kg (75 lbs) @ 100% force selection

Pusher Travel Rate: Minimum Maximum

0.18 µm/min

190.80 mm/min

Dimensions

10.16 x 30.48 x 21.59 cm (4 x 8.5 x 12 in) (H x W x D)

Weight

4.5 kg (10 lbs)

Regulatory Certifications CE, UL, CSA, CB Scheme, EU RoHS

18 18

Order #

Product

55-3305

Pulastile Blood Pump

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Electrospinning of Novel Scaffold Fiber Materials

Jet

Figure 3: Photograph of a meniscus of polyvinyl alcohol in aqueous solution showing a fibre being electrospun form a Taylor cone.

Syringe Pump

Pipette

Electrospinning

Polymer solution

Taylor cone

High Voltage Supply

Collector Screen (Rotating or Stationary)

Figure 1: Schematic of the Electrospinning setup.

A

B

C

D

Figure 4: Advanced Polymer Mixing Delivery System

E

Figure 2: (A) Vessel Scaffold, (B) Scaffold for Muscle, (C) Tissue Scaffold, (D) Bone Scaffold, (E) Organ Scaffolds.

In 1934, a process was patented by Formhals, wherein an experimental design was outlined for the production of polymer filaments using electrostatic force.

When used to spin less than 100 micron Fibers to micron fibers, the process is termed “electrospinning.” The electrospinning method uses a high voltage to create an electrically charged jet of polymer solution or melt, which then dries or solidifies to leave a polymer fiber. This is accomplished by first mounting a syringe into a syringe pump capable of delivering a constant pressure and smooth flow to a pulled spray nozzle. One electrode is placed into this spinning solution/melt while the other is placed onto a grounded collector, most often a metal wire mesh. The driving force is provided by a high voltage source generating up to 30kV of positive or negative polarity through the electrode located at the nozzle tip. When an electric field is subjected to the end of the nozzle tube a charged is induced on the surface of the liquid.

A mutual charge repulsion causes a force directly opposite to the surface tension, such that as the intensity of the electric field is increased, the hemispherical surface of the fluid at the nozzle tip elongates into a conical shape known as the Taylor cone. With increasing field, a critical value is attained when the repulsive electrostatic force overcomes the surface tension and a charged jet of fluid is ejected from the tip of the Taylor cone. The jet undergoes a whipping process during which the solvent evaporates and the remaining charged polymer fiber randomly lays itself on the grounded metal collecting screen. By adjusting the flow of the fluid from the pump and the magnitude of the electric field, the spinning rate can be controlled.

Variables: 1. Molecular Weight, Molecular-Weight Distribution and Architecture 2. Solution Properties: Viscosity, Conductivity, Surface Tension 3. Electric Potential, Flow Rate, Collection Screen 4. Distance between capillary and collection screen 5. Ambient Parameters: temperature, humidity, chamber air velocity 6. Motion of the Target Screen (collector) and chamber Air Velocity

The Harvard Apparatus Family of Pumps is well-suited for electrospinning: • Regulatory compliance ensuring electrical currents do not damage pumps with an option for a custom grounding cable for added user protection • Deliver the smoothest and most accurate flows so that uniform fibers are created without droplet formation • Programmable flow rates with automatic time control; timed length of spin, optimization protocols can be created, powerful enough for viscous solutions, highest accuracy at the lowest (nanoliter) flow rate range

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Electrospinning

• Rugged design with 2 year warranty and global support Order #

Product

70-3007

PHD ULTRA™ Programmable Syringe Pump

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

19

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

PHD ULTRA™ Push-Pull

Continuous Flow for Nutrient Delivery Systems

20

PHD ULTRA™ Push-Pull Programmable Syringe Pump • Compensating Flows - The control of continuous infusion and simultaneous withdrawal of liquids while monitoring fluid levels • Perfusion Across Tissue Beds - Directional control of pulseless flow across a tissue bed using switching valves • Continuous Flow with High Accuracy and Smooth Flow - Pump Any Volume Large or Small with Smooth, Non Pulsating Flow • Continuous Accurate Flow for High Pressure systems - Unlike peristaltic pumps, syringe pumps can pump against high pressures • Easily Sterilized Flow Path - By replacing syringe and tubing with a sterilized set, this pump can maintain it’s sterility

Figure 1: Pressure-driven flow was continous using a programmable push-pull syringe pump (Harvard Apparatus, Holliston, MA). Media was equilibriated with 10% or 21% O2 in a gas exchanger made with gas-permeable silastic tubing.

The continuous flow pump can provide low pulsation, high accuracy and precise delivery of flow to any reactor set-up. When you are trying to provide low shear stress, non pulsing flow during the adhesion step of regeneration of cell deposition for tissue or organ development, the syringe pump provide the continuous Flow compensation of a peristaltic, with higher accuracies and precisions. These flows can be cooled or heated with in –line heaters or coolers, These pumps have a rugged construction which allows the to work for long periods of time with high reliability. These pumps come with a two year warranty as a testament to their durability.

Order #

Product

70-3009

PHD ULTRA™ Push-Pull Programmable Syringe Pump

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Pressure 4

Pressure 5

Pressure 1

Pressure 2

Pressure 3

Pressure Delivery Injection System

Pressure Controlled Injection System

Figure 1: Pressure Delivery Injection System

•Simultaneously measure overall system pressure •Option for holding injection pressure constant for defined time period The system is specifically designed for repeatable, pressure controlled injections into intact organs. However a wide range of settings and accessories makes the system appropriate for virtuallly any semi-rigid substrate and certain hollow-body systems.

Figure 2: System components include a syringe pump, injection pathway, pressure measurement hardware, and data acquisition and feed back system. Injection parameters are programmed while pressure is continuously monitored. Real time pressure feedback modulates syringe pump to keep pressure constant.

Order # Product Pressure-Controlled Injection System Parameters Controller

Order # Product Constant Pressure Injection

77-0000

ML866 POWERLAB 4/304 Channel w/LABCHART Software to Measure System Pressure after Syringe

73-1523

PLUGSYS Minicase Type 609

73-2806

72-4496

Research Grade Blood Pressure Transducer, 115VAC/60Hz, with 6 ft cable

SCP PLUGSYS Servo Control F/Perfusion with Interface Cable for PHD ULTRA™ Control

73-0065

TAM-A HSE PLUGSYS Transducer Amplifier Module Type 705/1, Analog Display

72-9843

Pressure Catheter to Tam Cable

Blister Pressure Measurement 72-9845

1F SGL Pres CTH 120 cm No Rep

Syringe Pump Capacity 2X up to 140 ml Syringes Max Flow Rate with 2X 140 ml Syringes is 3.68 ml/sec per Syringe or 7.36 ml/sec

Accessories 72-2673

Y-Connector FLL/FLL/MLL (Rotating), Package of 25

70-3007

PHD ULTRA™ I/W Programmable

72-14553-WAY

FLL-FLL-FLL "T" CONNECTOR, POLYPROPYLENE, PK/25

70-3033

PHD ULTRA™ Analog Central Imput

73-0500

Lab Stand w.Heavy Triangular Base Plate 8 mm OD Rod, 30 cm Long. Also includes Acrylate. Block Clamp (73-0566) W/8 & 10 mm Opening

72-9523

MLL to MLL Connector

77-0156

Manual Press Cal Kit

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

•Directly measure pressure at site of injection

21

Stepped

Flow Rate

Mixture/Dose Delivery System

Complex Mixture/Dose Delivery System

Continuous

Ramped

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Time

22

Figure 1: Stepped, Continuous, and Ramped gradient.

The new Harvard Apparatus Syringe Pump Gradient System has the ability to easily program step and continuous concentration gradients from two or three solvents (Figure 1). The Harvard Apparatus syringe pumps are highly published for their superior flow accuracy and precise flow performance from ul/min to hundreds of ml/min . Figure 2 shows the shows the flow at 1000 and 500 ul/min. In both stem cell bolus injections and 3D reactor perfusion stream dosing, the Harvard Apparatus Gradient systems allows you to automatically deliver injections fluids or perfusion flow streams that can have variable % concentration automatically. These serial dilutions or solution changes are preprogrammed or signaled and initiated by an I/O event. In the case of injections you could automatically change the amount of cells in every injection by programming an increase in the concentration of cell solution in each injection, or by simply selecting a new pre-established method. In perfusions, a pH probe could signal for a different % composition of the perfusion stream, or dose a perfusion stream automatically. This could provide more buffering capacity or adjustment solution to the perfusion stream completely unattended.

Figure 2: 1000 nl/min & 500 nl/min with 250 µl syringe Measured on a Sensirion Flow Sensor (PHD ULTRA)

This new capability of Harvard Apparatus syringe pumps to completely deliver % composition changes automatically with high accuracy and precision flow provides a new economical tool to advance experiments and eliminate manual input for complex solution changes.

- Enzyme studies - Reaction dosing solutions - Drug infusion experiments

Figure 3: The following screen shows the set-up for a gradient.

- Nutritional infusion experiments - Mixing polymer in electrospinning - Chromatography & FIA systems

Order # Product 70-4101

PHD ULTRA Gradient System 1 Master/1 Satellite with Stand

70-4102

PHD ULTRA Gradient System 1 Master/2 Satellites with Stand

70-4106

PHD ULTRA Gradient System 1 Master/1 Satellite without Stand

70-4107

PHD ULTRA Gradient System 1 Master/1 Satellite without Stand

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Accessories

Syringe and In-Line Temperature Controllers

Syringe warmer on a syringe pump

SC-20 Dual In-line Solution Heater/Cooler

Accessories

Syringe warmer mounted on a support stand

• Heats and cools from 0° to 50°C • Compatible with Warner Series 20 Chambers • Optimized for use with the CL-100 Bipolar Temperature Controller

SWS-10, SWS-60 & SWS–140 Syringe Warmers • Independent temperature control for individual syringes

The SC-20 is designed to thermally regulate one or two solutions at the same temperature. Solution temperature can be maintained at 0°C at flow rates of 2 ml/min., 5°C at 5 ml/min., or as high as 50°C at 5 ml/min.

• Accommodates 10, 60 and 140 cc syringes • Scale marking ports permit volume monitoring during use • Can be powered from 12 volt battery for sensitive electrophysiology applications

An integral water jacket is used to remove excess heat from the SC-20 peltier device. Running water either from a tap or a large reservoir can be used. Flow rates as low as 4 liters per hour are sufficient to maintain cooling efficiency.

The SC-20 can be used with either one or two discrete perfusate solutions, or with a solution/gas combination. When coupled with a PHC Series Imaging Chamber Heater/Cooler Jacket, the SC-20 provides an effective means of temperature control in a Warner chamber, even in the absence of solution flow.

Each SC-20 is supplied with a TA-29 Thermistor Cable Assembly for monitoring the bath temperature during use, 10 feet of PE-160 tubing and 10 feet of 1/8" I.D. x 1/4" O.D. Tygon tubing.

Heater Resistance

18 Ω

Voltage Requirement

Variable to 12 V maximum

Temperature Range

Ambient to 65°C

Specifications

Temperature Accuracy

±1°C

Minimum Temperature

0°C (2 ml/min. max flow)

Cable Length

2.4 m

Maximum Temperature

50°C

Warranty

One year

Maximum Flow Rate at 5°C

5 ml/min.

Accuracy

±0.1°C

Syringe Warmer Model Specifications Model SWS-10 SWS-60 SWS-140

Weight 32.7 g 76 g 192 g

Length 38.2 mm 83.7 mm 109.5 mm

Internal Dead Volume

330 µl

Perfusion Lines

Type 316 Stainless Steel, 0.032 in ID x 0.062 in OD

OD

ID

Syringe Type

Water Jacket Ports

Type 316 Stainless Steel, 0.12 in ID x 0.147 in OD

22.2 mm

16.2 mm Dickerson

Becton

Controller

Model CL-100 Bipolar Controller

29.1 mm Dickerson

Becton

35.0 mm 51.0 mm

41.4 mm

Physical Dimensions:

Monoject

Body (D x L)

21 x 165 mm

Weight

109 g

Cable Length

Order #

Model

Product

64-1584

SWS-10

Syringe Heater for 10 cc Syringes

64-1560

SWS-60

Syringe Heater for 60 cc Syringes

64-1585

SWS-140

Syringe Heater for 140 cc Syringes

64-1545

TC-124A

Temperature Controller, 120 VAC US

64-1545E

TC-124AE

Temperature Controller, 240 VAC Europe

64-1655

TC-144

Temperature Controller

64-1606

BAC-1

Battery Adapter Cable

1.9 m

Connector Type

15 pin Male “D”

Warranty

One year

Order #

Model

Product

64-0353

SC-20 Solution Heater/Cooler Two Line

64-0352

CL-100 Bipolar Temperature Controller

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• Designed for use on a syringe pump or support stand

Specifications

In-line solution heating has proven to be one of the most effective methods of maintaining the temperature of perfusion solutions. The SC-20 Dual In-line Solution Heater/Cooler utilizes Peltier thermoelectric devices to regulate temperature both above and below ambient levels.

Replacement Parts 64-0107

TA-29 Cable with Bead Thermistor for Heater Controllers

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

23

Accessories

Accessories

Micro Valves & Micro Connectors Valves/Controllers (Fluids & Pressure) & Glass or Plastic Fluidic Chips

In-Line PEEK cartridge for Toxic removal

Manifold/Connectors, Needles & Tubing

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• Fast • Cost-effective • Preparation of microfluidic volumes Until now, commercially available options for preparing microliter volumes have suffered major drawbacks, including high back pressure, clogging, leakage and limited surface chemistry options. New CapTite cartridges offer customizable packing and re-use, for easy sample preparation in both prototypes and highthroughput, automated systems.

Micro Fluidic Circuits & Chips

Harvard Apparatus has a wide range of tubing, connectors and manifolds so you can create the fluid pathway to meet your specific requirements. We offer many types of tubing of different materials and sizes. Our fluid manifolds start at 2 channels and go all the way up to 24 channels. We have a multitude of connectors that allow you to complete the fluid connections and complete your system. Please call our technical support staff for additional information.

Microfluidic Construction Kit Qty Order # Model Product Single User Kit

72-0426

C360-KIT1

Microfluidic Construction Single User Kit for 360 µm capillary tubing

Kit Components: Includes Tools A, B, C, D, E (1 each) 75 10 5 5 10 25 2 2

• Compact components for 360 µm, 1/16, and 1/32 inch in tubing • Fast, breadboard-based design • Leak-free high pressure setups Routing fluids can be one of the most time-consuming tasks of microfluidics research. CapTite microminiature components take the pain out of constructing setups. Based on components developed by Sandia National Laboratories, these compact components for 360 µm and 1/16 in tubing are designed for leak-free connectivity, even at high pressures.

24

Tubing, Connectors and Manifolds

72-0431 72-0433 72-0438 72-0443 72-0449 72-0448 72-0445 72-0430

C360-100

One-Piece Fitting

C360-101

Plug

C360-203

Tee Interconnect

C360-204

Cross Interconnect

C360-300

Luer-Lock Adapter

C360-400

Bonded Port Connector

C360-500

Selector Valve

LS-600

Breadboard

C360-KIT2

Microfluidic Construction Interface Kit for 360 µm capillary tubing

Interface Kit

72-0427

Kit Components: Includes Installation Tools A, B, D, E (1 each) 50 5 5 5 10 2 2

72-0431 72-0433 72-0438 72-0443 72-0449 72-0445 72-0430

C360-100

One-Piece Fitting

C360-101

Plug

C360-203

Tee Interconnect

C360-204

Cross Interconnect

C360-300

Luer-Lock Adapter

C360-500

Selector Valve

LS-600

Breadboard

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus Ventilators & Anesthesia The World’s Most Published Ventilation Systems for Stem Cell Harvesting

Minivent for Mice

Inspira for Mice up to Cats

683 for Mice & Rats

• SUTURES AND NEEDLES • SCAPELS • FORCEP • STERILZERS • VITAL SIGN MONITORS • BLOOD FLOW • CAPNOGRAPH • EEG • GLUCOSE • HEART RATE • O2 • OXIMETERS • pH • PULSE RATE • TEMPERATURE

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• ANESTHESIA • CIRCUITS • SCAVENGERS • DEWARS • INFUSION PUMPS • ANIMAL PREPARATION TOOLS • ID SYSTEMS • HAIR REMOVAL • RESTRAINERS • ANIMAL TEMPERATURE CONTROL • MAGNIFICATION PRODUCTS • OPERATING TABLES • OPERATING LIGHTS • RECOVERY CHAMBERS • SURGICAL TOOLS

Accessories

Look for the complete line of products in the NEW Havard Apparatus Animal, Organ and Cell Physiology catalog.

Anesthesia Machine phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

25

Phase 2: Introduction

PHASE 2: Engineering & Modification Introduction to Phase 2 Tools, Equipment and Systems Cell, Tissue Engineering, Modification, Preparation & De-Cellularization 1. Cell or Tissue Modification: In Vivo, In Vitro, Gene Delivery - Pneumatic Femto Injectors - Electroporation - Electrofusion - Iontophoresis - Liposomes - Mechanical Injectors

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

2. Cell or Tissue Accessories

26

- Capillary Tubes - Capillary Pullers - Capillary Forges - Homogenizers - Manipulators

3. Perfusion Chambers - Imaging Culture Plates

4. Molecular Isolation, Purification Analysis - Amino Acid Analysis - Electrophoresis - Western Blot - Protein-Ligand Interactions - Protein-Protein Interactions - DNA purification Kits

5. Molecular Characterization - Spectrometers

PLI-100A femtoliter cell injector

Phase 2 Catalog and Guides

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

PHASE 2: Engineering & Modification

LIPOSOMAT-LIPOSOME PRODUCTION

ELECTROPORATION HIGH-THROUGHPUT IN VIVO, IN VITRO

ELECTROFUSION & ELECTROPORATION

ASK FOR A FREE GUIDE Send email to: regen@harvardapparatus.com

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

PLI-100A: CELL INJECTION SYSTEM

Phase 2: Introduction

Harvard Apparatus is the Leader in Femoliter to Microliter Cell Injection & Transfection Products for Optimal Cell and Tisssue Engineering

27

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Phase 2: Examples

PHASE 2:

Engineering & Modification, Application Examples

A Range of Application Areas for Phase 2 Tools, Equipment and Systems

MOLECULAR BIOLOGY

SAMPLE PREPARATION

• Electrophoreses (1D,2D) • Molecular Biological Sample Prep Tools - Chromatographic - Dialysis - Equilibrium Dialysis

• Spectrometers • HTS Plate Readers • Amino Acid Analysis

ASK FOR A FREE GUIDE Send email to: regen@harvardapparatus.com PROTEIN-DRUG BINDING STUDIES • No Force Flow • O2 Pressure CO2 • pH Calcium Urea • Temperature, Lactate & More

28

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

PHASE 3: Culturing & Growth This section contains systems and tools for the regeneration and growth of engineered or natural scaffolds and cellular constructs. Complete bioreactor systems typically include one or more growth chambers for the construct as well as fluid handling pathways for culture media perfusion and cell seeding. Probes for monitoring physiological parameters are typically included, though these are discussed in more detail among the Phase 4 products.

Phase 3: Introduction

Introduction to Phase 3 Tools, Equipment & Systems

Cell Culturing & Tissue Growth - Media Infusion Pumps a. physiological b. continuous flow - Imaging Chambers

3. Cell Sorting - Capillary Glass - Manipulators

5. 3D Bioreactors 6. Spinner Flasks 7. Centrifuges 8. Pipetting Systems 9. Tissue Culture 10. Incubators 11. Shakers 12. Continuous flow pumps

4. Cell Harvesting

Phase 1 Catalog and Guides

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

1. Cell & Tissue Preparation 2. Cell Growth

29

InBreath 3D Bioreactor for Hollow Organs, Bronchus, Trachea & Blood Vessels

Proven through Research and Human Clinical Transplantation InBreath

from the culture compartments. The connection between the motion unit and the culture chamber allows the first to remain in the incubator for the whole culture period, moving the chamber independently every time is needed (i.e. sampling, medium exchange). An external control unit regulates and monitors rotation. Autoclavability, ease of handling under sterile conditions, reliability and precision ensure the full compatibility of the device with the GLP rules (Good Laboratory Practice).

Benefits & Features

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• Facilitates cell seeding procedures on both sides of a 3D tubular matrix, ensuring homogeneous plating

30

• Proven Design - demonstrated regeneration of human bronchus and successful human transplantation with positive clinical outcome; as recently published by Macchiarini et al (2008) Clinical transplantation of a tissueengineered airway, The Lancet, Volume 372, Issue 9655 • Allow seeding and culturing of different cell types on either side of the tubular scaffold • Enhance oxygenation of the culture medium and mass transport (oxygen, nutrients and catabolites) between the medium and the adhering cells • Stimulates the cells with hydrodynamic stimuli, favoring the metabolic activity and the differentiation process • Allows the achievement and maintenance of sterility and other criteria of Good Laboratory Practice (GLP), simplicity and convenience • Permits the possibility of automation and scale-up/-out In Breath is a rotating, double-chamber, bioreactor designed for cell seeding and culturing on both surfaces of a tubular matrix and includes rotational movement of the scaffold around its longitudinal axis. A polymeric culture chamber houses the biologic sample and the medium for the whole culture period. Cylindrical scaffold holders are constructed with working ends of different diameters - to house matrices of diverse dimensions - and a central portion of smaller diameter to expose the luminal surface of the matrix for cell seeding and culturing. A co-axial conduit links the inner chamber to the external environment through an appropriate interface at the chamber wall.

This provides access to seed and feed the luminal surface of the construct.

Secondary elements moving with the scaffold holder induce continuous mixing of the culture medium to increase oxygenation and mass transport. The cell/matrix construct is moved by a DC motor (0-5 rpm adjustable) separated

Easy-to-use Controller • Compact design for remote placement (i.e. outside the incubator) • The rotational speed can be controlled from 0 to 5 rotations per minute • Motor overload indicator for safe operation • CE certified

Purpose-Built Reactor Chamber (Custom configurations available) • Reactor container and spindle are made of Polysulphone, Teflon and 316 Stainless Steel allowing for: - sterilization - chemical inertness - biological compatibility - transparent for excellent visualization • Chamber has quick-fit spindle for easy removal of the spindle and organ construct for analysis or dissambly • Rotating spindle assures even exposure to nutrient media • Integrated ports on spindle allow access to internal (lumen) surface of organ • Compact dimensions allow placements of multiple units within standard incubator • Offset cover allows for oxygenation of sample by non sheering ambient air contact • Quick release and disassembly of parts makes sterilization easy

phone 508.893.8999 • email regen@harvardapparatus.com • web www.harvardapparatus.com

InBreath 3D Bioreactor for Hollow Organs, Bronchus, Trachea & Blood Vessels (continued) InBreath

Figure 2: Sealable access port through the chamber wall creates a conduit to the hollow-milled tissue spindle enabling clean access to the bronchial lumen for seeding, in this case, with endothelial cells from the host.

Figure 3: The semi-circular “half pipe” chamber floor creates a shallow well for external seeding, |here with host chondrocytes derived from bone marrow stem cells of the host. Mixing posts on the rotating tissue spindle maintain cells in suspension for even deposition on the bronchial surface. (Figure 3)

Though it integrates several sophisticated features, the design of the InBreath is straightforward producing a very effective 3D Bioreactor.

Note: Images Courtesy of P. Macchiarini, University of Barcelona and S Mantero, Politecnico di Milano

Specifications

Figure 1: Tissue-specific tissue spindles ensure proper fit to the rotating core for even exposure to bathing media.

Rotational Speed

0 - 5 RPM

Diagnostics

Positional Monitoring

Materials

Polysulphone, Teflon, 318 Stainless Steel

Validation

CE

Power

100 - 240 VAC, 50/60 Hz

Order #

Product

73-4145

InBreath 3D Bioreactor for Hollow Organs, Bronchus, Trachea & Blood Vessels

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

InBreath

Note: For Research Use Only. Not for use in humans unless proper investigational device regulations have been followed.”

References: Macchiarini et al (2008) Clinical transplantation of a tissueengineered airway, The Lancet, Volume 372, Issue 9655

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31

NEW LB-2 Bioreactor for Mouse and LB-2 Bioreactor

Rat Lung Regeneration

LB-2 Bioreactor For regeneration and respiratory monitoring of Mouse and Rat Lungs from decellularized scaffolds

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Benefits & Features: • Proven design – demonstrated regeneration of rat lung with physiological performance and subsequent transplantation, as recently published by Ott et al, 2010, Regeneration and orthotopic transplantation of a bioartificial lung , Nature Medicine, Volume 16 No 8, pgs 927-933 •Active control of both airway ventilation and vascular perfusion during regeneration

based Organ Regeneration. Over 60 years of whole organ physiological perfusion experience are at the core of this first commercially available bioreactor for Lung Regeneration.

Like all Harvard Apparatus - Hugo Sachs Elektronik perfusion systems, the LB-2 provides unmatched physiological maintenance and monitoring capabilities thanks to the patented Solid State Physiological Perfusion Circuit (S2P2C) technology. In the LB-2, this means rigidly milled pathways, directly into the Perspex structure, for both vascular perfusion and airway ventilation. The result is precisely repeatable non-turbulent perfusion and respiration characteristics.

This combined with the naturally excellent thermal properties of Perspex, creates a system that allows control, maintenance, and monitoring of vascular and respiratory mechanics during regeneration in a way that is more physiologically relevant than any conventional bioreactor.

System Accessories: • Small Animal Ventilator

•On-line monitoring of respiratory mechanics and vascular physiology

• Pulsate Blood Pump

•Self-contained bioreactor specially designed for unique lung physiology

• Transducers

Physiological maintenance and monitoring:

• Data Acquisition and Analysis

• Syringe Pump • Amplifiers

The LB-2 provides an optimal, physiologically relevant environment for organ growth as well as the ultimate foundation for real-time monitoring of respiratory mechanics. The complete range of pulmonary monitoring parameters are fully integrated into the bioreactor to enable physiological monitoring during organ generation and end-stage validation for transplant.

• Airway Flow • Airway Pressure • Intrapleural Pressure • Tidal Volume

• Filtration Coefficient

• Minute Volume

• pH, pO2, pCO2

• Pulmonary Resistance

• Temperature

• Pulmonary Compliance

• Lung Weight

References: Ott et al (2010) Regeneration and orthotopic transplantation of a bioartificial lung, Nature Medicine, Volume 16 No 8

• Pulmonary Artery Pressure • Pulmonary Venous (Left Atrial) Pressure • Vascular Resistance The LB-2 represents the evolution of legendary Harvard Apparatus - Hugo Sachs Elektronik perfusion technology from acute ex-vivo perfusion to Bioreactor

32

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NEW LB-2 Bioreactor for Mouse and Rat Lung Regeneration (continued) Phase 1: Decellularization

Figure 1: Perfusion decellularization of whole rat lungs. (a) Photographs of a cadaveric rat lung, mounted on a decellularization apparatus allowing antegrade pulmonary arterial perfusion. pa, pulmonary artery; pv, pulmonary vein; tr, trachea; RUL, right upper lobe; RML, right middle lobe; LL, left lobe. Freshly isolated lung (left), after 60 min of SDS perfusion (middle), and after 120 min of SDS perfusion (right). The lung becomes more translucent as cellular material is washed out first from apical segments, then from the middle segments and finally from the basal segments.

Phase 2: Regeneration

Phase 3: Validation

Figure 2: In vitro functional testing of regenerated lung constructs. (a) Photographs of regenerated lung contructs attached to the isolated lung apparatus) pa, pulmonary arterial cannula; pv, pulmonary venous cannula; tr, trachea) in expiration (left) and inspiration (right; RUL, right upper lobe; RML, right middle lobe). (b) Bar graphs showing results of blood gas analyses of pulmonary venous effluent of native lung, HUVEC- and A549-seeded lung groups showed gas exchange function; pO2/FiO2 ratio and pCO2 did not differ between native and H-FLC-seeded lungs. (c) Line chart showing the dynamic pressure/volume relationship during five respiratory cycles of native, decellularized and regenerated lungs. Corresponding compliance values (ml/cm H2O/s are shown in boxes. (d) Bar graph comparing vital capacity of native (black), H-A549-seeded lung (gray) and HFLC-seeded, regenerated lung (red). Error bars, s.d.

Order #

Product

73-4213

LB-2 Basic Unit for Lung Bioreactor, Size 2

Note: Images and data courtesy of H. Ott, Massachusetts General Hospital

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Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Phase 4: Transplantation Figure 3: Orthotopic transplantation and in vivo function. (a) Photograph of left rat chest after anterior thoracotomy, left pneumonectomy, and orthotopic transplantation of a regenerated left lung construct. Recipient left pulmonary artery (A), left main bronchus (B) and left pulmonary vein (V) are connected to regenerated left lung pulmonary artery (a), bronchus (b) and pulmonary vein (v).

White arrowheads, the recipient’s right lung (infracardiac and right lower lobe); black arrowheads, the regenerated left lung construct. (b) Radiograph of rat chest after left pneumonectomy and orthotopic transplanation of a regenerated left lung constuct. White arrowheads, recipient’s right lung; black arrowheads, regenerated left lung construct. (c) Results of blood gas analyses showing decrease in arterial oxygen tension (PaO2) after left pneumonectomy and partial recovery after orthotopic transplantation of a regenerated left lung construct. Baseline, single lung, 10 min and 30 min measurements were obtained with the rat intubated with the extubated and breathing room air without support of a ventilator. Upper P values compare single lung ventilation to 5 min, and 30 min time points after transplantation at 5 min, 30 min and 6 h time points after operation. Error bars, s.d.

LB-2 Bioreactor

The LB-2 is a Complete Lung Bioreactor for all phases of Lung Regeneration

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Harvard Apparatus | Advanced Solutions for Regenerative Medicine

HPC-3

HPC-3, Hydrostatic Perfusion Chamber for Organ and Tissue Decellularization

34

Detergent or enzyme based decellularization of tissues and organs can be a cumbersome process, with multiple bottles of different solutions all leading to the tissue through a convoluted tubing network. The HPC-3 is a practical solution to this problem, adding useful features and enabling standardization of decellularization protocols for any tissue. Three independent non-heated glass vessels are mounted on a robust vertical stand to provide the required hydrostatic perfusion pressure and all required tubing, in appropriate lengths, is included. Glass vessels and the included Teflon Lids are fully autoclavable.

Order #

Product

73-001020

3-Fold Hydrostatic Perfusion Chamber with Stand

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Cell and Tissue Perfusion, Imaging & Assay Chambers

The NaviCyte Diffusion/Ussing Chamber Systems The “World Standard” for conducting substance transport studies across cultured cell monolayers or excised tissue under dynamic conditions.

• Higher Throughput Screening –Up to 24 Chambers • Easier to Use than Other Systems Applications Include: Drug Delivery Toxicology Pharmacokinetics Metabolic Studies

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Ideal for live cell imaging experiments and cellular electrophysiology

Cell & Tissue Perfusion

Universal Coverslip Chamber

Transport Studies

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35

Flow Through Devices for Removal of Toxins, Metabolites or Signaling Products Imaging Chambers

Now sterilizable, in-line dialysis system to de-toxify 3D bioreactor perfusate.

Take out DMSO, salts, and cell communication signal molecules so you can keep the 3D bioreactor perfusate in safe limits for organ regeneration The Flow-Thru DIALYZER is a unique system for the rapid dialysis of sample volumes from 20 µl to 100 ml. It provides a large surface area for Flow-Thru online dialysis with minimal sample loss. The entire dialysis unit is made of Teflon, an inert material, and has two separate serpentine channels superimposed on each other and separated by a dialysis membrane. The length of each channel is about 700 mm. Five different chambers are available (20 µl, 75 µl, 150 µl, 300 µl and 600 µl). Chambers of different volumes can also be superimposed on each other for specific applications.

Flow-Thru DIALYZER™ & Membranes A. Regenerated Cellulose MEMBRANES: 1k Da MWCO 2k Da MWCO 5k Da MWCO 10k Da MWCO 25k Da MWCO 50k Da MWCO Dialysis Buffer In

B. Cellulose Acetate MEMBRANES:

Dialysis Buffer Out

100 Da MWCO 500 Da MWCO

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Dialysis Buffer Chamber

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1k Da MWCO 2k Da MWCO 5k Da MWCO

Membrane (1.2 in x 3.2 in)

10k Da MWCO 25k Da MWCO 50k Da MWCO

Sample Chamber

100k Da MWCO

C. Polycarbonate MEMBRANES: 0.01 um 0.05 um 0.60 um

Sample Solution In

Sample Solution Out

Filtration or Solid Phase Extraction In-Line PEEK cartridge for Toxic removal

• Fast • Cost-effective • Preparation of microfluidic volumes Until now, commercially available options for preparing microliter volumes have suffered major drawbacks, including high back pressure, clogging, leakage and limited surface chemistry options. LabSmith’s new CapTite cartridges offer customizable packing and re-use, for easy sample preparation in both prototypes and high-throughput, automated systems.

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PHASE 4:

Physiological Assessement & Conditioning Monitoring

Monitoring and recording of Physiological and Metabolic parameters, or BIOSENSING, is critical in understanding the viability of organisms, organs, tissues and cells and is the ultimate determinant of organ validation for transplant.

Harvard Apparatus has probes and systems for more physiological and metabolic measurements than anyone else.

We have tried to list all of our BIOSENSORS and in the following pages, we have noted some of the newest.

For more details or for a specific request, please contact our technical specialists at regen@harvardapparatus.com.

Phase 4: Introduction

Introduction to Phase 4 Tools, Equipment & Systems

Cell, Tissue, Organ & Regenerative Systems Condition Monitoring Tools Sensors

1. Microscopic Imaging & Perfusion Chambers 2. Microscopic Environmental Controls 3. Electrophysiology Products 4. Cell Condition Monitors

8. Ion Sensors Ammonia, Calcium, Chloride, Ethanol, Glucose, Lactate, Nitrate, Nitric Oxide, Nitrite, Peroxide, Potassium, Sodium, Urea 9. Flow 10. Temperature 11. Pressure 12. Force 13. O2; CO2

Tissues 5. Isolated Tissue & Perfusion Baths

Organs 6. Lung Regeneration Bioreactor 7. 3D Bioreactors – Hollow Organs

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Cells

Capnograph

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37

Biosensing

A Range of Harvard Apparatus BIOSENSING Probes are Available for Virtually Any Metabolic or Physiological Measurement Application Areas

Control Unit

Spill Sensor Mats with Connecting Cable

• Respiratory Mechanics • Cardiovascular Physiology • Absorption • Metabolism • Excretion • Nutrition • Electrophysiology • Biopotentials • Animal Behavior

Battery Powered Spill Sensor System • Protect your microscope

• Dosing

• Audible alarm

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Measurement Probes

• TTL cutoff circuitry

• Blood Pressure Transducers

• Compatible with upright and inverted microscopes

• Force Transducers

• Easy set up and installation

• Differential Air Pressure Transducers

• Complete system

• Flow Probes • Capnographs • Pulse Oxymeters • Temperature Monitors • Thermocouples • ECG Electrodes • Monophasic Action Potential Electrodes • Weight Transducers • Electrical Stimulation Electrodes • Imaging devices

• Force Measurement

• Blood Gas Probes, pH, O2, CO2

• Digital Microscopy

• Ion & Compound Sensors - Ammonia - Calcium - Chloride - Ethanol - Glucose - Lactate - Nitrate - Nitric Oxide - Nitrite - pH - Peroxide - Potassium - Sodium - Urea

• Automated Blood Gas and Temperature Control • Air Pressure • pCO2, pO2, pH • Nitrate & Nitrite Ions • Oxygen levels in bulk fluids

• Infrared Motion Detectors

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NEW Optical Detection of pH, O2 and CO2 are

Ideal for Bioreactor Use

Optical Detection

Peel and Stick Sensors, iDots

Optical Biosensors • Completely Sterilizable sensors withstand CIP (Clean-In-Place), SIP (Steam-In-Place), gamma, and autoclave conditions.

Now pH, O2 and CO2 can be measured easily in disposable systems using new iDots. iDots are welded into the wall of the bioprocess container, are precalibrated, can be gamma sterilized and are made of all USP Class VI materials. iDots are readily adaptable to your single-use application using Polestar’s DSP series optical monitors.

Flow-cell probe is a zero dead-volume sensor that can be up with DSP series Optical Process Monitors for continuous measurement of DO or pH. Pre-calibrated DO or pH fluorescent sensing films deposited along the inner surface of the quartz flow-cell body enabling optical interrogation through the transparent wall of the flow-cell via standard fiber optic cable. A simple re-useable clamping device with integral fiber optic connector is provided to facilitate mounting the device. Flexible silicone tubing on each end of the flowcell enables quick connection to external tubing or adapters such as Luer-Lock fittings. All materials used in the construction of the flow-cell sensors are USP Class VI certified and can withstand autoclave or gamma sterilization.

Puncture probe oxygen sensors are configured for measurements of oxygen in applications such as package integrity testing, small volume samples and soft tissue monitoring. The probe is constructed from a small diameter fiber optic capped with a thin layer of oxygen sensing chemistry fixed within the lumen of a stainless steel hypodermic needle. Coring and non-coring designs are available in sizes ranging from 14 gauge to 24 gauge. Responses times (t90) for Polestar’s puncture probes are <10 seconds for measurements of gas phase oxygen and <20 seconds for dissolved oxygen.

Puncure Probe

Flow Cell

Bag Integrated Sensors

DSP Monitoring Unit

Designed to operate with oxygen, pH, and CO2 sensors, the DSP Series Monitors are the core of any process monitoring system. The DSP Series transmitters include automatic temperature and pressure compensation as will as a proprietary AGC (automatic gain control) to optimize readings and maximize sensor lifespan. The NEMA 12/4X IP 65 enclosure has water-tight cordgrips for brining in process temperature and pressure data via 4-20 mA signals, a simple and intuitive menu, and a high visibility VFD display. Capable of logging collected data in real time to virtually any data acquisition system.

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Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• USP Class VI-certified sensors are shipped precalibrated although the system allows the user to perform a simple standardization procedure.

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Perfusion Solution Monitor Harvard Apparatus | Advanced Solutions for Regenerative Medicine 40

Universal Perfusion Solution Monitor: proven electrochemical detection of pH, pO2, pCO2 in hydrodynamic systems PH, pO2, & pCO2 System

Universal Perfusion Solution Monitor

Detail of pH, pO2 & pCO2 Sensor System

Detail of pO2 Sensor

• Rugged construction • Noise free design, no cross talk • High sensitivity • Good linearity For studies involving isolated or in situ perfused organs (heart, lung, liver, kidney) it is often important to continuously monitor pO2, pCO2 and/or pH of the perfusate or effluate. Which parameter is measured depends on the organ used and on the type of experiment conducted.

The universal perfusion solution monitor permits precise continuous or discontinuous measurement in liquid media of these three key parameters pO2, pCO2 and pH. The electrodes are all side stream flow-through electrodes and require a pulsation-free roller pump to deliver constant flow of perfusate through the electrode at flow rates in the range from 0.5 to 2 ml/min. The solution is pulled through each of the electrodes in series. The pH sensor requires an external reference electrode (both the pO2 and pCO2 contain internal reference electrodes). A solid state leak free reference system is used.

Because of the high impedance of these sensors, screening or shielding of the measuring circuit is required to guard against electrostatic discharges and other electrical disturbances. The shielding case also provides a convenient point of attachment to the multi-electrode mounting plate. Sensors are available individually or configured as a complete package with suitable amplifiers and Data Aquisition.

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Guide for Advanced Metabolic and Physiological Evaluation of Organisms, Organs, Tissues & Cells using Infrared Contrast Imaging (IRCI) Microscopy

Physiological Fluidics Monitoring

Vascularization

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Disease Monitoring with Enhanced Visualization

41

Microscopy

Guide for Advanced Metabolic and Physiological Evaluation of Organisms, Organs, Tissues & Cells using Infrared Contrast Imaging (IRCI) (continued) Know More About Your Experiment with IRCI Harvard Apparatus has utilized infrared imaging in a way that not only provides temperature imaging but also produces high contrast images in biological and medical applications. This new technique uses the camera’s ability to detect IR emmisivity from objects as a function of their surface texture, composition, temperature, shape and more. This new utilization of infrared imaging has been called IRCI - Infrared Contrast Imaging. This powerful technology can visualize, with high resolution, experimental conditions based on tissue vascularization, structure or metabolic state.

• Site evaluations for viable or necrotic tissue • More clearly reveal morphological features of biological substrates • Track injection flow paths in real time to evaluate injection quality

Accurate Noncontact Infrared Measurement For Visualizing Physiological, Metabolic and Morphological features with IRCI To assure accurate noncontact infrared temperature measurement keep in mind the following: • Distance to Target (Spot) Ratio • Field of View • Environmental Conditions • Ambient Temperatures Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• Emissivity

Distance to Target Ratio The distance controls the energy hitting the detector, the closer the camera the more signal being picked up.

Field-of-view The array must have the correct lens in front of it to get as much illumination of the emitted light onto as many pixels as possible. When choosing a system the lens should be selected to give you the proper working distance and the magnification to display the area of interest on as much of the array as possible

Environmental Conditions Watch for environmental conditions in the working area. Steam, dust, smoke, etc., can prevent accurate measurement by obstructing the unit's optics. Dew point or the moisture in the air can cause blurring of the image also if you have glass containers or certain plastics they will absorb all the IR energy so they are not transparent to the IR and you will not be able to see through these. If this happens replace the material with a IR transparent material if possible.

Ambient Temperatures (the surrounding temperature) If the thermometer is exposed to abrupt ambient temperature differences of 20 degrees or more, allow it to adjust to the new ambient temperature for at least 20 minutes. The camera fixed-mounted sensors are specified for performance within certain ambient temperature ranges. For high ambient temperatures, some camera’s offers air cooling and water cooling options as well as accessories, such as Thermojacket.

Emissivity Emissivity is the measure of an object's ability to emit infrared energy. Emitted energy indicates the temperature of the object. Emissivity can have a value from 0 (shiny mirror) to 1.0 (blackbody).

Emissivity Differences Create Contrast with IRCI IRCI has the ability to enhance contrast of objects based on their emmissivity. There are many features of many different types of samples which may be revealed by IRCI, making it a particularly useful tool. Some of these features are:

• Surface Texture • Sample Thickness • Temperature • Chemical Composition • Depth Profile

42

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Guide for Advanced Metabolic and Physiological Evaluation of Organisms, Organs, Tissues & Cells using Infrared Contrast Imaging (IRCI) (continued) Infrared contrast imaging (IRCI) enables clear visizualization of physiological and structural features of living tissue which are otherwise very difficult to detect.

This is demonstrated in a rodent model where cardiac infarct was induced via ligation of the left anterior descending coronary artery (LAD).

Areas of advanced cardiac infarct generally display a faint and localized “paleness” at the epicenter of the infarct, where myocardial injury is greatest and most pronounced. The images below show, widespread changes in detected emmissivity on the myocardial surface after LAD ligation. What may be partially visible to an experienced observer using the naked eye, is now readily apparent to even the uninitiated user of IRCI technology. As a result, this technique enables routine procedures to be done without extensive training and with greater accuracy.

Rat Orientation

Infarct Area

Infarct Area

Infarct Area

Heart Time Zero to Infarct

Heart

Heart 30 Seconds After Infarct

One Minute After Infarct

Small, Compact and Powerful

• Pocket sized 3.5" LCD display/image capture

The Harvard Apparatus T-Series of portable infrared cameras takes infrared camera ergonomics, weight and ease-of-use to a new level. Usability is key

• Storage and playback • Up to 200X variable magnification

Ideal for visualizing changes in vascularization or surface features of tissue and organs with otherwise low contrast.

• Built-in 8-Always-On white LEDs lighting (Option near UV light) • Microscope Resolution 628 x 586 • LCD Screen Resolution 320 x 240

i40 / i50 / i60

IR Microscope

• Economical IR camera for monitoring temperature

• Imaging of 100 micron area at 3 micron resolution

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Harvard Apparatus | Advanced Solutions for Regenerative Medicine

Handheld Digital Microscope with IR illumination • Real time image or video

Microscopy

Application Example: IRCI for Evaluation of Stem Cell Injection Site Physiology

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Digital Microscopy 0-500X with Integral Illumination: Small, Powerful and Economical AM2011

Microscopy

• Handheld Microscope • 0.3M/Resolution 640x480 • USB 2.0 Output • 10~200X Various Magnification • 1/4" Color CMOS

AM412N AV/TV output AM412NT LED On/Off AM412NZT Polarize AM412NTL Enhanced WD AM412MNT Aluminium Alloy AM412MNTL Aluminium Alloy • Handheld Microscope

• Built-in 4 white LED’s

• 0.3M/Resolution 640x480 • AV (Video)/TV Output • Built-in 8 white LED’s

AM311SAM311ST (Micro Touch)

AM313/313T

• Handheld Microscope

• Handheld Microscope

• 0.3M/Resolution 640x480

• 0.3M/Resolution 640x480

• USB 2.0 Output • 10~200X Various Magnification • LED on/off controlled by software

• 10~200X Various Magnification • Measurement feature include (*)

• AM311ST with Trigger Button

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

• USB 2.0 Output

• Built-in 8 white LED’s

AMH-RUT • Handheld Iris Microscope • 1.3 MP • USB 2.0 Output

AM311H Earscope AMH-EAN AV/TV output AMH-EUT-V3 Earscope 1.3MP • Handheld Earscope

• Resolution 1280x1024

• 0.3 MP / 1.3 MP

• USB 2.0

• USB 2.0 Output / or TV

• Built-in 4 white LED’s • 10x -20x Various Magnification

• 10~50X / 55X~90X Magnification • Built-in 4/6 white LED’

AMH-N5UT

• Resolution 1280x1024

AMB-DT-U1 USB 5X~37X AMB-DT-U2 USB 10X~92X AMB-DT-T1 AV/TV Polarizer AMB-DT-T3 AV/TV 10X~92X

• USB 2.0

• Handheld Dental Scope

• Built-in 4 white LED’s

• 0.3 MP (TV) 1.3 MP (USB)

• 500X fixed Magnification

• USB 2.0 Output / or TV

• Handheld Iris Microscope • 1.3 MP

• Various Magnification

AMK4012-C200

• Built-in 8 white LED’

• Handheld Digital Microscope • Pocket sized 3.5" LCD display/image capture • Real time image or video Storage and playback • Up to 200X variable magnification • Built-in 8-Always-On white LEDs lighting (Option near UV light) • Microscope Resolution 628x586 • LCD Screen Resolution 320x240

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References Our extensive references cover all areas of interest for Regenerative Medicine • IMAGING • ISOLATED HEART SYSTEM • NANOFLUIDICS • STEM CELL INJECTIONS • STEM CELL COLLECTION AND HARVESTING • USSING CHAMBER • VENTILATING ANIMALS

References

APPLICATION GUIDES: • BILAYER WORKSTATION • BOLUS INJECTIONS • CELL INFUSION • ELECTRODE • CULTURE DISH FEEDING • DRUG INJECTIONS • ELECTROSPINNING SCALFOLDS Sample Research Publications:

1. Spontaneous hair cell regeneration in the mouse utricle following gentamicin ototoxicity ..BIOREACTOR K Kawamoto, M Izumikawa, LA Beyer, GM … - Hearing Research, 2009 - Elsevier... if missing hair cells can be replaced by a regenerative treatment ... Infusion speed was controlled by a syringe pump (Harvard Apparatus Inc., Holliston, MA) at the ...

2. Regenerative medicine bioprocessing: concentration and behaviour of adherent cell …BIOREACTOR BJH Zoro, S Owen, RAL Drake, C Mason, M … - Biotechnology and Bioengineering, 2009 interscience.wiley.com ... Received March 27, 2009; Accepted April 3, 2009 ... for primary cells typical in regenerative medicine. ... vertically onto a syringe Pump (PHD2000, Harvard Apparatus...

3. Towards microfabricated biohybrid artificial lung modules for chronic respiratory support…BIOREACTOR /Materials KA Burgess, HH Hu, WR Wagner, WJ … - Biomedical Microdevices, 2009 - Springer...

215 McGowan Institute for Regenerative Medicine, University ... Biomed Microdevices (2009) 11:117–127 121 ... PHD 2000 syringe pump (Harvard Apparatus, Holliston, MA ...

5. Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cell… BIOREACTOR KC Clause, JP Tinney, LJ Liu, B … - … Engineering Part C: …, 2010 - liebertonline.com... 16 Issue 3: May 26, 2010 Online Ahead of Print: February 26, 2010 Online Ahead ... Cell SortingAnalysis of Unsorted Muscle-Derived Stem Cells and Muscle-Derived Stem Cell-3DGB. ... 1 Hz,4 ms, 50–100 V, rectangular pulses) using a stimulator (Harvard Apparatus, Holliston, MA ...

6. BMI1 sustains human glioblastoma multiforme stem cell renewal…STEM CELL cjb.net [HTML]M Abdouh, S Facchino, W Chatoo, V … - Journal of …, 2009 - neuro.cjb.net... This work revealed that PcG proteins are required in human GBM to sustain cancer-initiatingstem cell renewal. Materials and Methods. ... The scalp was closed with wound clips (Harvard Apparatus). Animals were followed daily for development of neurological deficits. ...

7. In vivo multimodal imaging of stem cell transplantation in a rodent model of Parkinson's disease…STEM CELL J Jackson, C Chapon, W Jones, E Hirani, A … - Journal of neuroscience …, 2009 - Elsevier...

6-Hydroxydopamine (6-OHDA) was injected into the right striatum at a dose of 16 g, in a volume of 4 L at a rate of 1 L/min, using a micropipettor (Harvard Apparatus, Edenbridge, UK). ...Post-implantation stem cell localisation was assessed using high-resolution MR images. ...

8. Effects of ischemic preconditioning on regenerative capacity of hepatocyte in the …STEM CELL A Bedirli, M Kerem, H Pasaoglu, O Erdem, E … - Journal of Surgical Research, 2005 - Elsevier... model have shown that initiation of the regenerative response depends ... and the common bile duct with a microvascular clamp (Harvard Apparatus, Inc., Hollinston ...

9. Development of microfluidics as endothelial progenitor cell capture technology for …STEM CELL BD Plouffe, T Kniazeva, JE Mayer Jr, SK … - The FASEB Journal, 2009 - FASEB... using a Harvard Apparatus PHD 2000 syringe pump (Harvard Apparatus, .23 October 2009 ... EPCs are a potential tool in regenerative medicine and likely ...

10. Substantial detrusor overactivity in conscious spontaneously hypertensive rats with …STEM CELL LH Jin, KE Andersson, YH Kwon - Scandinavian Journal of Urology and Nephrology, 2009 informaworld.com... 2 Wake Forest Institute for Regenerative Medicine, Wake ... ISSN 1651-2065 online # 2009 Informa UK ... a micro- injection pump (PHD22/2000 pump; Harvard Apparatus). ...

11. Tbx3 improves the germ-line competency of induced pluripotent stem cells..STEM CELLS/ Electroporation J Han, P Yuan, H Yang, J Zhang, BS Soh, P Li, SL Lim, … - Nature, 2010 - nature.com... Received 14 February 2009; Accepted 9 December 2009; Published online 7 February 2010. ...analyses, Y. Loon Lee, P. Gaughwin and colleagues from the Stem Cell and Developmental ... Cell fusion by Electro cell manipulator (ECM 2001, BTX Harvard Apparatus) and incubated ...

12. Pilot study to investigate the possibility of cytogenetic and physiological changes in bio- …SCAFFOLD MATERIALS/ electrospinning H Kempski, N Austin, A Roe, S Chatters, SN … - Regen. Med., 2008 - Future Medicine... 3 s -1 (PHD 4400, HARVARD Apparatus Ltd., Edenbridge ... Materials for Tissue Engineering & Regenerative Medicine2,158 ... (2009) Bio-electrospraying embryonic stem cells ...

13. In vivo bone tissue engineering using mesenchymal stem cells on a novel electrospun …SCAFFOLD MATERIALS/ electrospinning M Shin, H Yoshimoto, JP Vacanti - Tissue engineering, 2004 liebertonline.com... Q 5 0.1 mL/min, PHD 2000 sy- ringe pump; Harvard Apparatus, Holliston, MA ... 2009. Nanostructured polymer scaffolds for tissue engineering and regenerative medicine ...

14. Silk fibroin microtubes for blood vessel engineering…SCAFFOLD MATERIALS nih.gov M Lovett, C Cannizzaro, L Daheron, B … - Biomaterials, 2007 - Elsevier... c Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School ... the bioreactor using a syringe pump (Harvard Apparatus, Holliston, MA ...

15. Quantitative Analysis of Neural Stem Cell Migration and Tracer Clearance in the Rat Brain by MRI…STEM CELL JA Flexman, DJ Cross, LN Tran, T Sasaki, Y Kim, S … - Molecular Imaging and … Springer... DOI: 10.1007/s11307-010-0311-3 Mol Imaging Biol (2010) ... morphologically transplanted cell migration in the rat brain and parametrically estimate neural stem cell migration speed. ... the skull) using a picoliter syringe pump (Pico Plus Syringe Pump; Harvard Apparatus; Holliston ...

16. Therapeutic effect of genetically engineered mesenchymal stem cells in rat experimental leptomeningeal glioma model. STEM CELL C Gu, S Li, T Tokuyama, N Yokota, H Namba - Cancer letters, 2009 Elsevier... Mesencult ® murine mesenchymal stem cell medium) after aspiration by 5 ml syringe connected with 23 G needle ... the subarachnoid space by a 50 µl microsyringe (Hamilton Company, Reno, NV) connected with a 27 G needle and a microinjector (Harvard Apparatus, Inc., South ...

16. Magnetosonoporation: Instant magnetic labeling of stem cells… STEM CELL B Qiu, D Xie, P Walczak, X Li, J Ruiz- … - Magnetic …, 2010 - interscience.wiley.com... VC 2010 Wiley-Liss, Inc ... basal medium (Stemcells Inc., Vancouver, Canada), adding NeuroCult supple- ments for neural stem cell differentiation and ... Using a nanoinjector (Harvard Apparatus, Holliston, MA), the left brain hemispheres were locally implanted with approximately 8 ...

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

4. An automated system for delivery of an unstable transcription factor to hematopoietic stem …BIOREACTOR E Csaszar, G Gavigan, M Ungrin, C Therien, … - Biotechnology and Bioengineering, 2009 interscience.wiley.com... Ontario, Canada 7 McEwen Centre for Regenerative Medicine, University ... ß 2009 Wiley Periodicals, Inc ... a Model 33 Twin Syringe Pump (#553333, Harvard Apparatus, St ...

17. A seeding device for tissue engineered tubular structures BIOREACTOR L Soletti, A Nieponice, J Guan, JJ Stankus, WR Wagner … - Biomaterials, 2006 - Elsevier... The tees were connected to a precision syringe pump (Harvard Apparatus Inc.,Holliston, MA, USA) outside the chamber by means of hydraulic rotating joints (DeublinCo., Waukegan, IL, USA) and polyvinyl chloride (PVC) tubing. ...

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45

References

References

18. A novel culture system shows that stem cells can be grown in 3D and under physiologic pulsatile conditions for tissue engineering of vascular grafts 171.65.102.190….BIOREACTOR [PDF]O Abilez, P Benharash, M Mehrotra, E … - Journal of Surgical …, 2006 - Elsevier... system that would allow future testing of mechanical, biochemical, and thermal stimuli on stem cell differentiation into ... (B) Photo of the bioreactor (left) and ... The pulsatile pump was a Harvard Apparatus Model 1405 (Harvard Apparatus, Holliston, MA) modified for computer control...

19. … Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization…BIOREACTOR M Lovett, D Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation,and ... Medford, MA 02155. Email: Received: April 19, 2010 Accepted: June 1, 2010. ... syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA ...

20. A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cells BIOREACTOR KC Clause, JP Tinney, LJ Liu, B … - … Engineering Part C: …, 2010 - liebertonline.com... Activated Cell Sorting Analysis of Unsorted Muscle-Derived Stem Cells and Muscle-Derived Stem Cell-3DGB. MDSC-3D collagen gel bioreactor (MDSC-3DGB) construction ... 1 Hz, 4 ms, 50–100 V, rectangular pulses) using a stimulator (Harvard Apparatus, Holliston, MA). ...

21. Fabrication and Characterization of Prosurvival Growth Factor Releasing, Anisotropic Scaffolds for Enhanced Mesenchymal Stem Cell Survival/Growth and Orientation …SCAFFOLD MATERIAL F Wang, Z Li, K Tamama, CK Sen, J Guan - Biomacromolecules, 2009 - ACS Publications... It has been shown to enhance survival of many cell types including smooth muscle cell, cardiomyocyte, and mesenchymal stem cell.(21-23) Besides, IGF-1 ... The gelatin/BSA/IGF-1 solution was fed at a rate of 0.2 mL/h by syringe pump A (Harvard Apparatus) into the inner tubing. ...

22. Fabrication and Characterization of Prosurvival Growth Factor Releasing, Anisotropic Scaffolds for Enhanced Mesenchymal Stem Cell Survival/Growth and Orientation …SCAFFOLD MATERIALS F Wang, Z Li, K Tamama, CK Sen, J Guan - Biomacromolecules, 2009 - ACS Publications... It has been shown to enhance survival of many cell types including smooth muscle cell, cardiomyocyte, and mesenchymal stem cell.(21-23) Besides, IGF-1 ... The gelatin/BSA/IGF-1 solution was fed at a rate of 0.2 mL/h by syringe pump A (Harvard Apparatus) into the inner tubing. ...

23. Porous nanocrystalline silicon membranes as highly permeable and molecularly thin substrates for cell culture …SCAFFOLD MATERIAL AA Agrawal, BJ Nehilla, KV Reisig, TR Gaborski, DZ … Biomaterials, 2010 - Elsevier... Received 8 March 2010; accepted 16 March 2010. Available online 15 April 2010. Abstract. ...An open perfusion microincubator (Harvard Apparatus) was used to maintain cells at 37 °C and mineral oil was floated on top of the media to minimize evaporation. ...

24. A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning… SCAFFOLD MATERIAL nih.gov [HTML]F Teulé, AR Cooper, WA Furin, D Bittencourt, EL … - Nature protocols, 2009 - nature.com... Bacteria have quicker generation times and do not require a very sophisticated laboratory setup,thus reducing time and expenses. Additionally, the production can be easily scaled-up using bioreactors. ... no. P-259); Harvard Pump 11 plus single syringe (Harvard Apparatus, cat. ...

25. Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials …SCAFFOLD MATERIAL nisco.ch [PDF]SN Jayasinghe, N Suter - Biomicrofluidics, 2010 - link.aip.org... Biomicrofluidics 4, 014106 (2010)]. ... glass microslides, while for scanning electron microscopy (SEM) and transmission electron microscopy (TEM), fibers were collected ... at the lowest possible flow rate allowed by our present syringe pump, (PHD4400, Harvard Apparatus Ltd., Kent ...

Harvard Apparatus | Advanced Solutions for Regenerative Medicine

26. Characterization and tensile strength of HPC-PEO composite fibers produced by electrospinning …SCAFFOLD MATERIAL L Francis, A Balakrishnan, KP Sanosh, E Marsano - Materials Letters, 2010 Elsevier ... strength of HPC-PEO composite fibers produced by electrospinning, Materials Letters (2010), doi: 10.1016/j ... PEO, Sigma Aldrich, Mw = 10,000) were used as starting precursors to fabricate nano fibers. ... of 250 m and was mounted on a syringe pump (Harvard Apparatus serial n A ...

27. Tbx3 improves the germ-line competency of induced pluripotent stem cells..STEM CELL/ Electroporation J Han, P Yuan, H Yang, J Zhang, BS Soh, P Li, SL Lim, … - Nature, 2010 - nature.com ... karyotype analyses, Y. Loon Lee, P. Gaughwin and colleagues from the Stem Cell and Developmental ... h, inactivated feeder cells and fresh media were added, and the culture was then ...2-cell fusion by Electro cell manipulator (ECM 2001, BTX Harvard Apparatus) and incubated ...

28. Osteochondral Interface Tissue Engineering Using Macroscopic Gradients of Bioactive Signals …STEM CELLS NH Dormer, M Singh, L Wang, CJ Berkland, MS … - Annals of biomedical …, 2010 Springer ... 0090-6964/10/0600-2167/0 © 2010 Biomedical Engineering Society ... There is a growing interest in hUCMSCs as a mesenchymal stem cell source, as they have been ... in a con- trolled manner using programmable syringe pumps (PHD 22/2000, Harvard Apparatus, Inc., Holliston ...

29. Engineered Early Embryonic Cardiac Tissue Increases Cardiomyocyte Proliferation by Cyclic Mechanical Stretch via p38-MAP Kinase Phosphorylation …BIOREACTOR nih.gov [HTML]KC Clause, JP Tinney, LJ Liu, BB Keller, … - … Engineering Part A, 2009 - liebertonline.com ... 1 Hz, 4 ms, and 70–100 V) using a stimulator (Harvard Apparatus, Holliston, MA). ... Pasumarthi, KB, Soonpaa, MH, and Field, LJ Myocyte and myogenic stem cell transplantation in ... A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle ...

30. Dynamic Seeding of Perfusing Human Umbilical Vein Endothelial Cells (HUVECs) onto Dual-Function Cell Adhesion Ligands: Arg-Gly-Asp (RGD)− Streptavidin and …BIOREACTOR CC Anamelechi, EC Clermont, MT Novak, WM … - Langmuir, 2009 - ACS Publications... combined a dynamic seeding system with a bioreactor proliferation phase to increase cellular adhesion and proliferation for three-dimensional polymer ... The dynamic seeding system consisted of a 10 mL syringe mounted on a Harvard Apparatus pump, infusing cells onto Teflon ...

31. Dynamic Seeding of Perfusing Human Umbilical Vein Endothelial Cells (HUVECs) onto Dual-Function Cell Adhesion Ligands: Arg-Gly-Asp (RGD)− Streptavidin and …BIOREACTOR CC Anamelechi, EC Clermont, MT Novak, WM … - Langmuir, 2009 - ACS Publications... combined a dynamic seeding system with a bioreactor proliferation phase to increase cellular adhesion and proliferation for three-dimensional polymer ... The dynamic seeding system consisted of a 10 mL syringe mounted on a Harvard Apparatus pump, infusing cells onto Teflon ...

32. Regeneration and orthotopic transplantation of a bioartificial lung-BIOREACTOR HC Ott, B Clippinger, C Conrad, C Schuetz, I … - Nature Medicine, 2010 - nature.com ... Received 22 December 2009 Accepted 04 July 2010 Published online 13 July 2010 ... black arrowheads), axial (gray arrowheads) and septal (black arrows) elastic fibers in decellularized ...trachea and was placed in a sterile, water-jacketed organ chamber (Harvard Apparatus). ...

33. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes sbi.org …BIOREACTOR [PDF]BJ Kane, MJ Zinner, ML Yarmush, M Toner - Anal. Chem, 2006... 1. A lowmagnification micrograph of an entire well is shown in Figure 5. Also on post-seeding day 1, the cell culture medium was ... These syringes were loaded into a programmable pump capable of supporting 10 individual syringes (PHD2000, Harvard Apparatus, Holliston, MA ... Cited by 53 - Related articles - All 9 versions 34. … Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization …BIOREACTORS M Lovett, D Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and ... the collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA ...

35. Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and … BIOREACTOR M Lovett, D Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... tube embedded within the collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA). For oxygen measurements,a custom housing for the bioreactor and oxygen measurement probe was ...

36. Cardiac tissue engineering using perfusion bioreactor systems …BIOREACTOR nih.gov [HTML]M Radisic, A Marsano, R Maidhof, Y Wang, G … - Nature protocols, 2008 - nature.com... only within the construct, that is, at the construct outlet oxygen concentration stops varying as a function of bioreactor length ... 97063A134), coated with PTFE by Microsurfaces; Stereomicroscope; Sterilization pouches; Syringe pump (Push/Pull; Harvard Apparatus or WPI Instruments ...

37. Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and … BIOREACTOR M Lovett, D Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... Medford, MA 02155. E-mail: Received: April 19, 2010 Accepted: June 1, 2010. ... 4 µL/min) of the silk tube embedded within the collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA). ...

38. Distribution of NTPDase5 and NTPDase6 and the regulation of P2Y receptor signalling in the rat cochlea nih.gov …BIOREACTOR [HTML]MG O'Keeffe, PR Thorne, GD Housley, SC Robson, … Purinergic …, 2010 - Springer... Purinergic Signalling (2010) 6:249–261 251 Page 4. ... Plastic tubing was placed inside the inlet and outlet holes and both tubes were connected to separate Hamilton syringes secured on a push–pull syringe pump (Harvard Apparatus, USA). ...

39. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes sbi.org…BIOREACTOR [PDF]BJ Kane, MJ Zinner, ML Yarmush, M Toner - Anal. Chem, 2006 -These syringes were loaded into a programmable pump capable of supporting 10 individual syringes (PHD2000, Harvard Apparatus, Holliston, MA). ... demonstrated that cocultured primary hepatocytes and fibroblasts in a flat plate bioreactor were not adversely effected by wall ...

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