Utilisation and management of white clover (Trifolium repens) for dairy cows in pastures

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Agronomy and Crop Improvement

Utilisation and management of white clover (Trifolium repens) for dairy cows in pastures

Introduction The utilisation of pastures for grazing largely depends on careful monitoring of conditions and their effect on crops and livestock, and the flexibility in management to optimise production. The main constraints on dairy pasture productivity are (i) the unpredictable varying seasonal conditions (rainfall, temperature fluctuations) (Campbell and Bryants, 1978), (ii) vegetation sensitivity to abiotic stress, competition (Sanderson et al., 2003), diseases, pests, trampling (Drewry et al., 2008), (iii) soil composition (Greenwood and McKenzie, 2001) and (iv) correct application of fertilisers (Andrews et al., 2007). The introduction of white clover (WC, Trifolium repens, Fabaceae) into pasture-based dairy production systems as fresh feed or silage, has the potential to (1) increase milk yield and (2) decrease nitrogen (N) fertiliser application (Lane et al., 2000; Lane et al., 1997; Caradus et al., 1996; Chestnutt and Lowe, 1970). Milk yield per dairy cow between 2001-2002 and 2011-2012 has increased by 18% on average but the number of dairy cow numbers declined by 19% (DairyCo, 2012) in the UK. Overall, the total milk production on dairy farms has decreased by 4% (Andrews et al., 2007). Therefore, improving dairy pastures in a cost-effective and sustainable way is crucial to sustain milk production in the UK. For example, in New Zealand, the financial contribution of white clover to the economy reaches the total of $3.1 billion (Caradus et al., 1996) through nitrogen fixation, forage yield, seed production and honey production. The major biotic constraints of WC is to maintain an optimal clover content within a pasture (Harris et al., 1997) while it has numerous competitors such as grasses, weeds, pests, diseases in the field while sustain its growth under trampling pressures by the cows. This essay reviews the major benefits of WC introduction in dairy pastures, requirements for its establishment, and effects of different management practices and briefly discusses appropriate crop protection and breeding strategies. Main benefits of WC compared to grass monoculture dairy pastures One of the major benefits of WC is its ability to fix nitrogen with Rhizobium trifolii. It is estimated that 200-300 kg N per annum can be fixed by WC inclusion which varies on the WC content within the pasture and it can save ÂŁ125-160 ha-1 annum-1 on a field (Andrews et al., 2007). Numerous studies have investigated the effect of N fertiliser and have shown that 400 kg N/ha application negatively effects clover growth and morphology (Harris et al., 1996), while 150 kg N/ha application has shown to increase clover content, but 250 kg N/ha and 200 kg N/ha had no significant effect under low (3.2 cows/ha) and high (4.5 cows/ha) stocking rates (Clark and Harris, 1996; Egan et al., 2018). McDonagh et al. (2017) found that WC on a mixed pasture with 100 kg N/ha under 21 grazing days yielded 1009 kg dry matter (DM)/ha more than on 250 kg N/ha pure perennial grass pasture with 30 grazing days throughout the whole year. Therefore, WC reduces the cost of N fertilisation by its N fixing ability and produces higher yields than ryegrass pastures with high amounts of fertilisers.


Agronomy and Crop Improvement

Milk yields have also been shown to increase by 3kg d-1 on average on pure WC swards compared to perennial ryegrass swards (PRG) (Wilkins and Munro, 1998). This depends on clover content - 50% of total DM of WC content increased milk yields by 22% (13.4 l/cow/day) and 75% content increased milk yields by 33% (13.8 l/cow/day) for both ad libatum and restricted herbage allowances (Harris et al., 1997). This could be explained by better nutritive values in terms of crude protein content, low structural fibre and better organic matter (OM) digestibility (Thomson et al., 1985; Harris et al., 1998), the increase in yield is mainly caused by higher voluntary intake (Bertilsson and Murphy, 2003, Ribeiro Filho et al., 2005).

Requirements for white clover establishment Sowing WC in spring has been shown to be more effective with the possibility of resowing in the autumn (Elgersma and Schlepers, 2003). The ideal soil conditions for WC establishment requires a soil pH around 6.0, and applying phosphorus (between 16-140 kg P/ha-1 annum-1) (Andrews et al., 2007; Singh et al., 1999), potassium and little or no N to ensure optimal soil fertility (Elgersma and Schlepers, 2003). Significant differences have been shown in a three year study between an 8 kg/ha and 3 kg/ha sowing rates (Kelly et al., 2006) on their establishment and survival long-term. Lower, 2 kg/ha sowing rates with suboptimal emergence (30-40%) rate could lead to only a 30% survival (Brock and Kane, 2003). Increased row spacing (36 cm from 18 cm) also increased clover DM yield by 42% (Annicchiarico and Tomasoni, 2010) while another study by Muto and Martin (2000) showed that sowing by specialised Hunter seeder increased clover content and establishment compared to conventional Vredo seeder at 20 cm row spacing. Due to the smallness of the seeds, it requires shallow (1-2-3 mm) seeding (Brock and Kane, 2003) and should be pre-inoculated with Rhizobium (type B) (Hayman and Mosse, 1979). The aim of clover establishment is 150 WC plants per m2 after three months of sowing (Haggar et al., 1985). It is crucial to take clover development and morphology into account in terms of management and supporting its growth (Figure 1).

Figure 1. White clover goes through quiescence during the winter, enters primary growth in the spring, during grazing and cutting of the summer months, it is in a regrowth phase (Brock et al., 2000). After flowering in July, it enters vegetative growth phase in the autumn. The morphology of the plants significantly changes in between season as well as in between seedling, tap-rooted and clonal phases.


Agronomy and Crop Improvement

Key issues with optimum utilization and grazing management As there are no conclusive studies about dairy cow foraging behaviour for WC (Ribeiro Filho and Peyrand, 2005; Cosgrove et al., 1996), maintaining high content (Rutter et al., 2004; Harris et al., 1997) and sward height (Rutter et al., 2002; Rook et al., 1994) within a pasture is the most important objective in order to benefit from the potential increase in milk yields as well reducing the risk of bloating (Rutter et al., 2004). By combining different grazing management strategies (Table 1) in between seasons, the WC content can be manipulated according to plant development and nutritional values. Deferred grazing (Harris et al., 1999) and lower ryegrass tiller density (Brock and Hay, 1996) at the beginning of the establishment could significantly promote WC establishment. Ayres et al. (1998) found that post-flowering, OM digestibility decreased 0.0016 d-1 until ripe seed stage and it is crucial to promote daughter tiller formation (Matthew et al., 1991) by decreasing the number of competitive grasses (Thomas, 1984) and weeds (Seefeldt et al., 2005). The damaging effect of trampling on WC development also increases with stocking rates, especially combined with suboptimal soil conditions (Tuohy et al., 2015; Menneer et al., 2005), soil moisture stress (Lucero et al., 1999; Turner, 1991) and low earthworm populations (Hay et al., 1987).

Table 1. Overview of different grazing management strategies, their finding and particular advantages. Management

Findings

Advantages

Reference

Set stocking

Comparing WC content in 56,000 tillers/m2 and 11-15,000 tiller/m2 ryegrass densities Deferring summer grazing by 25, 50, 75, 100

Continual removal of flower heads enables daughter tiller formation and growth

Brock and Hay, 1996

60% higher survival for WC stolons for 50100 day deferral

Harris et al., 1999

Cutting

Comparing rotational grazing with cutting with N fertiliser addition

Schils et al., 1999

Zero grazing

Giving 200, 500, 800 g of WC/kg of total DM

Average annual DM yields were 13.4 t ha-1 with rotations and 12.82 t ha-1 with cutting and a second silage cut increased WC cover by 8% Daily intake, milk yield, solids and fat increased at 500 g but not at 800 g

PRG, WC and Red clover silages

WC was superior to RC and PRG, modifying the milk fatty acid profile

Van Dorland et al. 2008

Strip grazing

Pure grass and mixed sward grazing, recording sward heights

Enriquez-Hidalgo et al., 2014

Rotational

Comparing WC content in 56,000 tillers/m2 and 11-15,000 tiller/m2 ryegrass densities Plots containing 25% and 75% WC by ground area and pure PRG

Diurnal feeding pattern, during the autumn ruminating time decreased, rumen pH was higher for mixed swards, and foraging behaviour was significant at 18% clover content At 5,000 tillers/m2, there is little competition between WC and ryegrass During the day, WC was partially preferred (63.2%) on the 25% content pastures but showed a clear diurnal feeding behaviour

Rutter et al., 2004

Deferred grazing

Facilitating diurnal feeding

Harris et al., 1998

Brock and Hay, 1996


Agronomy and Crop Improvement

Pasture protection and WC breeding Some varieties are better at competing with weeds than others (e.g. Giga) (Annicchiarico and Proietti, 2010b). Herbicides such as carbetamide, paraquat and propyzamide have significantly increased WC content (89% with 1-2 kg/ha propyzamide) in the spring and led to 70% reduction is grass growth (Haggar and Bastian, 1980). Over 250 cultivars have been developed dating back to the 16th century (Lane et al., 2000) and a 6%/decade of genetic improvement, 1%/year genetic gain for yield and 2.3-7.3% /cycle for nematode resistance has been accomplished (Jahufer et al., 2002) in WC breeding. There are numerous WC plant pathogens risking pasture health which has been in the frontline of fungicide improvement and breeding programmes (Figure 2) (Woodfield et al., 1996; Pederson and Pratt, 1995; Skipp and Watson, 1987). Disease resistance in WC varieties can greatly vary (Voisey et al., 2001; Burdon, 1980). Other patterns (e.g. leaflet width, mean stolon number, plant height, root density) (Lane et al., 2000; Woodfield and Caradus, 1994; Rhodes et al., 1994; Caradus et al., 1989; Thomson, 1865), production (DM yield, seed production) (Woodfield and Caradus, 1994) and chemical compositions (water soluble carbohydrates, tannins, cyanogenesis) (Higgs et al., 2009; Caradus et al., 1995; Thomson et al., 1985) are traits to evaluate within breeding strategies. The future of WC breeding is suggested to focus on winter hardiness (Frankow-Lindberg, 2001; Dalmannsdรณttir et al., 2001), ozone stress (Hofmann et al., 2001; Heagle et al., 1991), and water stress (Annicchiarico, 2004; Marshall et al., 2001; Brock and Kim, 1994).

Table 1. White clover diseases in the UK, New Zealand (NZ) and Australia. a) Black/sooty blotch (Cymadothea trifolii, Lewis and Thomas, 1991), b) White clover mosaic virus (WClMV), c) Clover rust (Uromyces nerviphilus) pustules, d) Pepper spot (Leptosphaerulina trifolii), e) Ascochyta leaf spot (Ascochyta sp.) f) Clover root weevil (Sitona lepidus), g) Argentine stem weevil larvae (Listronotus bonariensis), and h) Rust.


Agronomy and Crop Improvement

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Utilisation of White Clover (Trifolium repens) in dairy systems

Major benefits The introduction of white clover (WC,Trifolium repens) into pasture-based dairy production systems as fresh feed or silage, has the potential to (1) increase milk yield and (2) decrease nitrogen (N) fertiliser application.

Milk yields have also been shown to increase by 3kg day -1 on average on pure WC swards compared to perennial ryegrass swards (PRG).

More benefits

How to sow it?

?Soil structuring? by taproots can overcome problems of soil compaction

- Treat the soil with N, P, K and adjust soil pH to 6.0 - Sow in spring or autumn - Drill with 30-40 cm row spacing, with 3-5 kg/ha with shallow seeding (1-2 mm) - Target: 150 plants per m2 after three months of sowing

Cows consume 20 ? 30% more white clover than grass hence the milk yield production

Nutritional values and varieties - Choose a productive and persistent white clover variety - large-leaved, ladino varieties for silage and low stocking, medium to most dairy pastures (small ones are mostly for sheep) - Annual clover yield 30% greater from IBERS-bred varieties - Increased digestibility of clover leads to higher voluntary intake and hence increased milk yields


Utilisation of White Clover (Trifolium repens) in dairy systems Clover content - Maintain at least 30 - 40% of clover content within the pasture - 40 - 50% clover content will reduce risk of bloating - Keep sward height at > 4 cm - To reduce clover diseases, keep sward height < 6 cm - Keep ryegrass tiller density at 5 - 6,000 tillers/m2

Grazing management - Graze frequently ? approx. every 3 weeks at covers of no more than 1400 kg DM/ha - Rotational grazing: 5 - 21 grazing days - Set stocking/ continous grazing: during flowering time - Zero-grazing: silage cut in vegetative growth - Facilitate diurnal feeding (clover in the morning, ryegrass in the evening)

Monitoring morphology, growth and content - The main structural of a clover is the stolon which consists of nodes and internodes. - Each node produces one trifoliate leaf, a lateral bud and two nodal root buds. - Promote stolon production by reducing trampling and overshadowing by ryegrasses and weeds.


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