Bulletin issue 12

GREENFEED

BULLETIN ISSUE 12

EFFECTIVE MICROORGANISMS WITHIN GREENFEED SLOW RELEASE FERTILZER (E-BIOTIC)

contents, 2015

As the pioneer in Slow Release Fertilizer within the Southeast Asia Region, Greenfeed Group have relentlessly continues to provide significant improvements into the agricultural industry whether locally or globally. Each of this effort signifies the objectives of Greenfeed Groups through Greenfeed Slow Release Fertilizer; which is to foster the current practices within this industry such as oil palms towards the advanced precise sustainable agricultural practices. Such as the previous volumes, this series of bulletin will continue to be the channel of education and knowledge sharing to the public in order to increase their awareness in relating topic on the advantages of

Features

Greenfeed Slow Release Fertilizer. Thus, within each volume of Greenfeed Bulletin, studies carried out and updated can be seen published for reference to the reader as to what is slow release

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Effective Microoragnisms within Greenfeed Slow Release Fertilizer (E-Biotic)

fertilizer and the benefits that can obtain through this Modern High Efficient Fertilizers.

As such, these Greenfeed Bulletins will be sent to respective clients with the aim to keep them updated with the current progress of

Editor-in-chief Muhammad Asyraf Abdul Aziz

Greenfeed Slow Release Fertilizer and also distributed to the public as necessary

Technical Research and Development

Editor Shahirulikram Shaharuddin Strategy and Marketing

Grasping the torch to bring changes into auspicious industry, Greenfeed Groups continues to provide a platform for knowledge sharing between the company and respective clients as well the public. This Greenfeed Bulletin is part of the effort to share and clarify the noteworthy advantages of Greenfeed Slow Release Fertilizer.

Malaysian establishes Greenfeed Groups in Malaysia,, for Malaysian and soaring onto the global scale with achievements that will make Malaysia proud. Guided and motivated by the principle of “Passion with Innovation�/ this Greenfeed Bulletin will be achieved by this Malaysian company. Striving towards excellence will be experienced for betterment of the future

All rights reserved. No part of this publication may be used or reproduced in any form or by any means, including but not limited to electronic or mechanical photocopying, recording or by any information storage or retrieval system or otherwise, without prior agreement and written permission from the publisher.

Disclaimer The view or options contained in this publication do not necessarily reflect the policy and stand point of Greenfeed Group and Greenfeed Group will not liable or responsible towards any losses experienced by any parties on performance or non-performance based on information in this publication.

R E S E A R C H

&

D E V E L O P M E N T

Greenfeed offers an entire suite of analytical testing. We are dedicated to toward providing accurate, unbiased analysis of current products. The Lab provides easy-to-understand reports for concerning irrigation water, leaves, soil, and plant tissue. Greenfeed Professional Lab is staffed by expert technical advisors and talented in-house personnel to make up the manpower component of the company known to be the industry's most successful slow release fertilizers company. These individuals represent a wide array of scientific disciplines, including Chemical Engineering, Chemistry, Agronomy, Plant Pathology, Microbiology, and Mechanical engineering.

M A N U F A C T U R I N G Our commitment is towards producing reliable and consistent slow release fertilizer products. Our products are formulated under the most stringent quality standard in ensuring the nutrient/active ingredient; product stability; efficiency and ease of application. Slow release fertilizers are engineered to provide a specific release characteristic that has been proven through laboratory trials, field trials and customer experiences. Our Slow Release Fertilizer was tailored with formulation to suite the crops growth and climatic situation.

Intellectual Property

the Ministry of Trade of

Intellectual Property

Trade and Industry

Development Authority

Corporation of Malaysia

the Republic of Indonesia

Office of Singapore

Pioneer Status

Manufacturing License: A 015437

Industry Design No: MY07-00700-0101

NPB No.: 103-124-131208

Manufacturing

PI20044120 / PI20061653 / PI20061654 /

SNI No.: 02-2803-2000

Slow Release Fertilizer

PI20061655 / PI2011005234 / PI2011005235

Ministry of International

Malaysia Investment

MS ISO/IEC 17025

10th Global Super Excellent Brand

Malaysia Power Brand Awards 2014

TESTING

Award 2014 ~ 2015

Established Brand Awards

SAMM NO. 694

PCT/SG2006/000106 KR/10-2008-7029052 INDO/w00200803828

Korean Intellectual Property Office

Komite Akreditasi Nasional

Patent Registration Number

No. Akreditasi: 320/LS-PRO/SNI/2013

10-120381, 40-0695064, 40-0695065

SUMMARY: INTEGRATION OF LACTOBACILLUS spp, SACCHAROMYCES spp, AND BACILLUS NATTO AS EFFECTIVE MICROORGANISMS (EM) IN GREENFEED® SLOW RELEASE FERTILIZER PRODUCT

1.0 INTRODUCTION The use of Effective Microorganisms (EM) in agriculture is opening up new dimensions. Both for organic businesses and in the conventional agricultural industry, Effective Microorganisms (EM) is providing a wide range of possible applications. The Effective Microorganisms (EM) promotes the vitalizing forces of nature and is particularly influential in the anaerobic sections of the soil and the plant. They provide consistent benefits, which particularly in areas where problems with decomposition and pest infestation occur. Effective Microorganisms (EM) creates an environment that represses pathogenic germs and counteracts environmental pollution. With the help of Effective Microorganisms (EM), it is possible not only to cultivate more fertile soil and more resistant flora and fauna, but also to produce high-quality foods that are particularly rich in antioxidants.

Figure 1.01 Flow chart organic matter transformation with existence of Effective Microorganisms (EM) Coupled with Greenfeed® Slow Release Fertilizer’s unique pa patented technology as platform, a breakthrough in fertilizer’s industry were attained as stable symbiotic interactions between organic or and inorganic substance were made possible through this breakthrough technology. Within this summary paper, brief collective findings are presented in term of consistent evaluations carried out over a period of 6 months from 15th January 2015 until 14th June 2015 carried out by Technical Research Development at various trial locations involving mainly immature planting planting. This summary includes Effective Microorganisms (EM) colonies viability / lifespan, physiological analysis, vegetative analysis analysis, foliar content analysis, and soil content analysis.

The objective of such monitoring effort is to assess the effectiveness of Effective Microorganisms (EM) introduced with G f d® Sl l F ili ffrom various i ’ point of view. Within this summary, the assessment were carried out Greenfeed® Slow R Release Fertilizer agronomy’s in term of colonies’ viability / lifespan, physiological analysis, and soil content analysis. The collective results can be referred to in the later section within this summary.

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GREENFEED BULLETIN ISSUE 12, 2015

2.0 SUMMARIES AND REVIEW A series of efficacy studies on Greenfeed Slow Release Fertilizer with E-Biotic were carried out pertaining on various aspect relating to commercial planting. The studies were carried out on different grounds involving immature planting oil palm planting. This observation was initiated from 15th January 2015 and early conclusive results were obtained in comparative to other treatments. Each observation included of a series of Treatment 1 – Greenfeed® Slow Release Fertilizer with E-Biotic, Treatment 2 – Greenfeed® Slow Release Fertilizer without E-Biotic, Treatment 3 – Commercial Compound Fertilizer, and Treatment 4 – Placebo; these methodologies are observations based on field-testing. As for colonies viability study, the observations carried out were mostly in local warehouse and client’s storage facility as this is to observe the viable storage lifespan of the colonies introduced to Greenfeed® Slow Release Fertilizer.

SUMMARY 2.01 Effective Microorganisms (EM) Viability Studies

Plate 2.01 Effective Microorganisms (EM) colony formation count from Greenfeed® Slow Release Fertilizer with E-Biotic sample after 6 months storage From Month 1 to Month 3, the decreasing pattern was less precipitous as compared to from Month 3 to Month 5.

Decreasing count for both samples within respective period were 17.33 CFU / Month (Local Storage – Month 1 to Month 3), 32.33 (Local Storage – Month 3 to Month 5), and 11.5 CFU / Month (Local Storage – Month 5 to Month 6); whereas 19.33 CFU / Month (Plantation Storage – Month 1 to Month 3), 31.00 (Plantation Storage – Month 3 to Month 5), and 5.5 CFU / Month (Plantation Storage – Month 5 to Month 6).

Furthermore, viable Effective Microorganisms (EM) minimum colony count should resides within 220 CFU – 250 CFU per 60 nugget as this is to ensure effectiveness and to avoid over colonization that would results in adverse effect to the planting requirement.

Figure 2.01 Colony formation count of Greenfeed® Slow Release Fertilizer with E-Biotic over span of 6 months Figure 2.01 indicates monthly Effective Microorganisms (EM) colony count obtained from samples at different storage locations. The samples were taken periodically and straining process were carried out accordingly to determine the viability of E-Biotic technology introduced to Greenfeed® Slow Release Fertilizer. Based on Figure 2.01 above, a slight decreasing pattern was observed from samples obtained from local warehouse – manufacturing facility, and plantation’s storage system. The storage methodology was carried out according to published Greenfeed® Slow Release Fertilizer User Manual.

SUMMARY 2.02 PHYSIOLOGICAL ANALYSIS Physiological Analysis was carried out as part of the assessment to monitor the effectiveness of E-Biotic introduction to Greenfeed® Slow Release Fertilizer. The analyses involved were Photosynthesis Rate Analysis and Chlorophyll Content Analysis. The comparative analysis involved four (4) different treatments, which were Treatment 1 – Greenfeed® Slow Release Fertilizer with E-Biotic, Treatment 2 – Greenfeed® Slow Release Fertilizer without E-Biotic, Treatment 3 – Commercial Compound Fertilizer, and Treatment 4 – Placebo. The results presented can be referred to as follows:

GREENFEED BULLETIN ISSUE 12, 2015

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2.02.1 Average Photosynthesis Rate Analysis on Immature Oil Palms Receiving Different Treatment Figure 2.02.1 indicates monthly average photosynthesis rate analysis carried out immature oil palms over a period of 6 months. The 4 (four) differing treatments were evaluated and contrasted during this period and results indicated that immature oil palms applied with Greenfeed® Slow Release Fertilizer with E-Biotic performed significantly better as compared to the rest. The expected optimum threshold was within 14.5 μmol/m2/sec to 30.0 μmol/m2/sec and most of the observed immature oil palms receiving different treatments were within the expected threshold. Treatment 1 which includes E-Biotic technology consisting of Effective Microorganisms (EM) recorded higher average when compared to Treatment 2, Treatment 3, and Treatment 4; which was 22.1%, 52.9%, and 77.0% respectively. Hence, with E-Biotic introduction to Greenfeed® Slow Release Fertilizer, the plants indicated better photosynthesis rate as compared to other treatments. Furthermore, in term of chlorophyll content analysis, similar results were observed and this can be referred to Figure 2.02.2 below.

Figure 2.02.2 indicates monthly average chlorophyll content analysis carried out immature oil palms over a period of 6 months. The 4 (four) differing treatments were evaluated and contrasted during this period and results indicated that immature oil palms applied with Greenfeed® Slow Release Fertilizer with E-Biotic performed significantly better as compared to the rest. The expected optimum threshold was within 65.00 SPAD to 85.00 SPAD and most of the observed immature oil palms receiving different treatments were within the expected threshold. Treatment 1 which includes E-Biotic technology consisting of Effective Microorganisms (EM) recorded higher average when compared to Treatment 2, Treatment 3, and Treatment 4; which was 2.10%, 19.09%, and 22.97% respectively. Hence, with E-Biotic introduction to Greenfeed® Slow Release Fertilizer, the plants indicated better chlorophyll content as compared to other treatments.

SUMMARY 2.03 FOLIAR ANALYSIS / CRITICAL NUTRIENT CONTENT ANALYSIS The nutrients involved within Critical Nutrient Analysis carried out was Nitrogen (N), Phosphorus (P), Potassium (K), Magnesium (Mg), and Boron (B). The presented Critical Nutrient analysis showed higher readings attained by early mature oil palms applied with Greenfeed® Slow Release Fertilizer with E-Biotic as compared to the remaining fertilizer treatment replicates. The average critical nutrient level performances were recorded on periodical term of 3 months. The average results can be referred to as follows Table 2.03 Average critical nutrient level comparison between different fertilizer treatments Fertilizer Treatment Treatment 1 Greenfeed® Slow Release Fertilizer with E-Biotic Treatment 2 Greenfeed® Slow Release Fertilizer Treatment 3 Compound Fertilizer Treatment 4 Placebo

Nitrogen (N)

Phosphorus (P)

Potassium (K)

Magnesium (Mg)

Boron (B)

2.85

0.20

1.24

0.37

26

2.67

0.19

1.13

0.33

21

2.43

0.15

1.04

0.25

17

2.16

0.14

0.97

0.19

14

*Nitrogen (N), Phosphorus (P), Potassium (K), and Magnesium (Mg) - % on dry matter ** Boron (B) – part per million (ppm)

2.02.2 Average Chlorophyll Content Analysis on Immature Oil Palms Receiving Different Treatment

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GREENFEED BULLETIN ISSUE 12, 2015

Overall, early mature oil palm applied with Greenfeed® Slow Release Fertilizer with E-Biotic recorded a higher critical nutrient level performance for Nitrogen (N), Phosphorus (P), Potassium (K), Magnesium (Mg), and Boron (B) at 2.85% on dry matter, 0.20% on dry matter, 1.24% on dry matter, 0.37% on dry matter, and 26 ppm respectively. Based on the brief monitoring effort carried out over the period, the overall comparisons indicated that planted early mature oil palm receiving Greenfeed® Slow Release Fertilizer with E-Biotic technology recorded slightly higher readings as compared to the comparing fertilizer treatments.

2.03.1 Average Nitrogen (N) Nutrient Content Analysis on Immature Oil Palms Receiving Different Treatment

2.03.2 Average Phosphorus (P) Nutrient Content Analysis on Immature Oil Palms Receiving Different Treatment

2.03.3 Average Potassium (K) Nutrient Content Analysis on Immature Oil Palms Receiving Different Treatment

2.03.4 Average Magnesium (Mg) Nutrient Content Analysis on Immature Oil Palms Receiving Different Treatment

2.03.5 Average Boron (B) Nutrient Content Analysis on Immature Oil Palms Receiving Different Treatment

3.0 CONCLUSION Such monitoring efforts were still being carried out with expected research timeline of 24 months for both internal and on site observation. From such presented summary, it was concluded Greenfeed速 Slow Release Fertilizer performances improved significantly with the integration of Effective Microorganisms through E-Biotic technology attained by Greenfeed Group of Companies. Such effect was observed through physiological aspects and critical nutrient aspects when compared a range of fertilizer treatments. Furthermore, initial integration process proven that the Effective Microorganisms (EM) were able to sustain the colony numbers even after 6 (six) moths of storage within local warehouse or client based warehouse. Furthermore, based on progressive observation, the Effective Microorganisms (EM) were able to sustain even after undergoing commercial logistics processes as to replicate global delivery scenario.

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This significant achievement marks a new pioneering capability in High Efficient Fertilizer sector whereby dual amending properties are achievable through balanced integration of organic to inorganic substance resulting to a noteworthy synergistic platform in fertilizer industry. Such technology can be incorporated in any GreenfeedÂŽ Slow Release Fertilizer products that are currently commercially manufactured.

4.0 APPENDICES Appendix A – Microorganisms Content Lactobacillus spp.

Saccharomyces spp.

Bacillus Natto.

Strains of bacteria Ecto-mycorrhiza Endo-mycorrhiza Organic Medium Carrier

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GREENFEED BULLETIN ISSUE 12, 2015

: >109 CFU/g : >109 CFU/g : >109 CFU/g : 9 : 9 : 9 : Yes

Novelt y

Technological

Intr oduction

Greenfeed able revolutionized Gree ree eenfee nfeed nfee ed da are rre e ab ble ble le to to int in iintroduce ntro ntr tro rod od duc duc uce ce a re rev evo vol olut olu utio tion oni nize niz zed ed achievement achievem em ment me nt that that will th wiilll propagate wil prop opa pag ga atte ate e agriculture agric ag riicu cu ulttu ultu ure e int iinto in nto to o a whole new level. Achieving synergistic equilibrium in organic and inorganic elements, Greenfeed Group is now proud to introduce Greenfeed E-Biotic Technology into the commercial fertilizer industry. E-Biotic consists of

Greenfeed® ActiveWeed technological system utilises synergistic platform without causing development impairment to the plant. c The acting mechanisms inhibits pre-emerging weed growth within the plant’s vicinity. Hence, w p pl planters, farmers, and gardeners will be able to carry out “feed and weed” activity simultaneously without any hassle. Promoting better agronomy efficiency towards greener practice. This is attainable without harming the p environment, resulting in a much more sustainable practice.

improved selected microorganisms that have resounding amending properties that are beneficial to both soil and plant’s growth. Current Greenfeed Slow Release Fertilizer has been proven effective for commercial planting and with intergration of E-Biotic, fertilizer industry has been brought onto a whole new level.

The principle behind Greenfeed® POLITECH is to introduce alternative attractor that could attract the E. kamerunicus to the oil palms with the intention on further assist pollinating progression. The most suitable candidate for the selection of attractant is Estragole or methyl Chavicol (a natural non-hazardous and environmentally friendly organic compound) which is widely known as main attractant of E. kamerunicus.

These incorporation has allow Greenfeed® fertilizer to attract more substantial of E. kamerunicus to the palms for further pollination process.

Greenfeed® Hydrocell H20+ is a hydro format retaining fertilizer that comes with polymeric membrane that is capable of absorbing water, holding it and releases via the methodology of slow release. This product is designed in a special form of high pressed nugget 16g ~18g respectively, the enriched hydrocell within will not only help plant to develop a stronger rooting mass for better nutrient intake. It also have the capacity to retain and stored water in the environment where water is scarce.

I-Trace will emit luminescence properties that will allow planters to carry out residual tasks such as monitoring the nutrient supply status of the fertilizer as well as decision-making. The luminous characteristic of I-Trace capability was achieved through the realisation of the luminescence reaction that occurred instantaneously. The reaction is known as photoluminescence, which is a process known as resonant radiations involving absorption and emission of photons. The period between absorbing the energy to the emission of photon occurred for as rapid as 10 nanosecond.

Greenfeed Group © 2015 . All right reserved Nothing in this publication may be reproduced in whole or inpart, by any means, without the express permission from Greenfeed Group All specification lsited here are subjected to changed without prior notice.