Hunor Farkaš, Svetlana Ćujić, Jog Raj, Zdenka Jakovčević, and Marko Vasiljević PATENT CO, DOO., Vlade Ćetkovića 1A, 24 211, Mišićevo, Serbia 45th Mycotoxin Workshop, June 2–5, 2024, Vienna, Austria
OBJECTIVE
This abstract presents a multi-mycotoxin sample preparation and analysis method for EU regulated and emerging mycotoxins with Agilent 6460c UHPLC-MS/MS.
MATERIALS & METHODS
The method is based on “dilute and shoot” principle.
Sample preparation involves:
1. Extraction with an organic solvent.
2. Centrifugation of the extracts.
3. To compensate the matrix effects in electrospray ionization, the extracts are mixed with isotopically labelled internal standards (9) for each group of mycotoxins.
The addition of the internal standard is performed in the needle of the autosampler, before injection into UHPLC-MS/MS
Method validation in terms of linearity, selectivity, sensitivity, accuracy, and precision was performed in 8 complex feed matrixes (corn, compound feed, wheat, barley, soya meal, wheat bran, sunflower meal, total mixed ration) to detect 34 mycotoxins:
Aflatoxins B1, B2, G1, and G2
α - zearalenol, β - zearalenol, zearalanone, and zearalenone
Diacetoxyscirpenol
HT-2 toxin and T-2 toxin
Neosolaniol
3-acetyl deoxynivalenol, 15- acetyl deoxynivalenol, deoxynivalenol, and deoxynivalenol-3-glucoside
Nivalenol
Fumonisins B1, B2, and B3
Fusaric acid
Moniliformin
Fusarenon X
Ochratoxin A
Beauvericin
Enniatins A, A1, B, and B1
Citrinin
Patulin
Ergosine
Ergocryptinine
Alternariol
The limits of quantitation (LOQs) were from 0.05 ngmL-1 to 4 ngmL-1.
