Multi Mycotoxins Analysis Method for Animal Feed Samples Agilent

Development of a multi Mycotoxins

ANALYSIS METHOD for animal feed samples using Agilent 6460 LC-MS/MS

Mycotoxins, secondary metabolites of moulds, are commonly found in food and feed samples.

These mycotoxins are toxic to humans and animals.

Therefore, it is necessary to test animal feeds for mycotoxins contamination.

Farkas H.1 , Raj J.1, Cepela R.1, Vukovic G.2 and Vasiljevic M.1 PATENT CO, Vlade Ćetkovića 1A, 24 211 Mišićevo, Serbia 2 Institute of Public Health of Belgrade, Belgrade, Serbia

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STUDY AIM The aim of this work was to develop a fast and simple UHPLC-based multimycotoxin method for the determination and accurate quantitation of all mycotoxins regulated in feed (EU Directive 2002/32/EC, 2006/576/EC and 2013/165/EU) by liquid chromatography coupled with tandem mass spectrometry.

METHOD RESULTS

The method is based on “dilute and shoot” principle. It involves two step extraction and centrifugation of the extracts.

The blank samples were spiked with a mixture of 11 mycotoxin standard on two level (LOQ and 10 LOQ) in 12 replicates.

To compensate the matrix effects in electrospray ionization the extracts

The RSDr of the method were between 2.5% and 13.4% and the

are mixed with [13C] labelled

apparent recoveries were between 62% and 115% for all analytes.

internal standards for each group of mycotoxins (13C AB1, 13C DON, 13C ZON, 13C OTA, 13C FB1 and 13C T-2) before injection onto LC-MS/MS. The method was successfully

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CONCLUSIONS

validated on: Corn Compound feed

It is concluded that the “dilute and

Wheat

shoot” method with addition of [13C]

Barley

labelled internal standard is capable for

Soya meal

determining all EU regulated mycotoxins

Wheat bran

in animal feed and compound feed.

Sunflower meal TMR

PARAMETERS Method performance parameters were obtained by in-house validation.

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