Aflatoxin in Milk

AFLATOXIN M1 DETERMINATION in Milk Samples using LC-MS/MS

Hunor Farkaš, Jog Raj, Jelena Leković, Robert Čepela, Jasna Bošnjak, Marko Vasiljević PATENT CO. DOO, Vlade Cetkovica, Serbia

XVIII International Symposium Feed Technology (Food Tech Congress) Novi Sad, Serbia., (2018) page no 15 (Oral presentation).

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STUDY Aflatoxin B1 is the most common contaminant found in peanuts, cottonseed meal, corn, and other feed/food grains.

The Aflatoxin B1 is absorbed in the gastrointestinal tract of the animals and is biotransformed primarily in the liver, by hydroxylation forming derivatives such as aflatoxin M1 which can be excreted through body fluids, such as milk, and in eggs as well.

AIM

RESULTS

The aim of this work was to develop a fast and simple UHPLC-based

The average recovery of aflatoxin M1 at the different levels

method for the determination and

0.01, 0.05, and 0.25µg/kg varied between 79%-99%.

accurate quantitation of Aflatoxin M1 regulated in food (EU Commission regulation (EC) Nº 1881/2006)

The method/curve is linear from 0.01µg/kg to 0.5µg/kg Aflatoxin M1 levels.

liquid chromatography coupled with tandem mass spectrometry.

CONCLUSIONS MATERIALS & METHODS The milk samples were extracted with acetonitrile and deproteinated using centrifuge.

The blank milk samples were

A sample of ERM® - BD284 whole milk powder was tested using this method. The certified level of AFM1 in this sample was 0.44 µg/kg and the AFM1 level obtained using this method was 0.46 µg/kg, which varifies the accuracy of this method.

spiked with Aflatoxin M1 standard at three levels (LOQ, 5xLOQ, and 25xLOQ).

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