Decellularized Plants and Cross-linked Gelatin for Vascular Repair 1 1 Nicole Gorbenko , Nick Merna 1School of Engineering and Applied Science, Hofstra University
Results
Introduction
• 2mm grafts were created using gelatin, glutaraldehyde, acrylic rod, and decellularized leaf
• Over 95% of DNA removal was measured • Leatherleaf has highest elastic modulus 16
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Representative image of a rolled 3D graft 12
Leatherleaf Spinach
Preconditioning of 3D Graft
Parsley
• Regulates fluid flow, pressure, and air flow
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Elastic Modulus (MPa)
• Coronary artery disease is the leading cause of death in the United States • Lack of autologous vessels creates a demand for naturally or synthetically derived vascular grafts • Small diameter vascular grafts have been developed to replace complete or partially blocked vessels • <6mm diameter grafts have not been successfully developed using a synthetic material • 2mm graft was developed with the use of natural materials
Fabrication of 3D Graft
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Non-decellularized
Methods • Leatherleaf viburnum was decellularized using SDS and clearing solution (0.1% Triton X-100 and 10% bleach in deionized water)
SDS
SDS/EGTA
Tergitol/EGTA
Tergitol/SDS
Endothelialization Of Leaves • Rat aortic endothelial cells seeded onto decellularized leaves showed good viability and adhesion within the first five days • Cell seeding density and coverage was optimized to provide full coverage
Representative image of bioreactor setup with pulsatile pump, chamber and reservoir
Leatherleaf Viburnum prior to any treatment
Pressure waveform which the 3D graft experiences
Acknowledgements
Leatherleaf Viburnum after 72 hours in SDS
(A) SEM image of SDS-decellularized leatherleaf with no cells. (B) SEM Image of SDS-decellularized leatherleaf seeded with endothelial cells. Leatherleaf Viburnum after 6 hours of clearing solution
Research reported here was supported by the National Institute Of Biomedical Imaging And Bioengineering of the National Institutes of Health under Award Number R15EB033168. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.