6.7
Research and other work on the Verticillium problem in hops: Molecular detection of Verticillium directly in the bine using realtime polymerase chain reaction (PCR)
Sponsor:
Bayerische Landesanstalt für Landwirtschaft, Institut für Pflanzenbau und Pflanzenzüchtung (Bavarian State Research Center for Agriculture, Institute for Plant Production and Plant Breeding) AG Züchtungsforschung Hopfen (WG Hop Breeding Research)
Financing:
Erzeugergemeinschaft HVG e. G. (HVG Hop Producer Group)
Project Management:
Dr. E. Seigner
Team:
AG Züchtungsforschung Hopfen (WG Hop Research): P. Hager, R. Enders, A. Lutz, J. Kneidl
Collaboration:
AG Pflanzenschutz im Hopfenbau (WG Hop Plant Protection): S. Euringer, K. Lutz
Breeding
Dr. S. Radišek, Slovenian Institute of Hop Research and Brewing, Slovenia Duration:
Starting in 2008 to December 31, 2023
Combating Verticillium wilt in German hop growing regions is a long-term task. Research and the advisory function of the LfL, as well as the implementation of preventive measures by hop growers, are of central importance in the common fight against Verticillium in hop gardens. Objectives In addition to conventional phytosanitary or other cultivation techniques, planting Verticillium-free material is key in preventing Verticillium wilt from spreading through hop growing regions. Since 2013, hop seedlings have been tested for Verticillium using a highly sensitive PCRbased detection method. This is to ensure that only wilt-free hops are included in the LfL's testing program and then passed on to the Gesellschaft für Hopfenforschung (Society for Hop Research) (GfH), and from there to hop growers. Method Molecular detection directly in the bine using real-time PCR according to Maurer, Radišek, Berg and Seefelder (2013). Based on systematic research, it was possible to establish a very reliable and highly sensitive molecular detection technique for Verticillium directly in the hop bines (Maurer et al., 2013). This detection system uses a Multiplex TaqMan®-based real-time PCR method. It has been used in practice since 2014. As described by Maurer et al. (2013), the test was first applied on V. nonalfalfae and V. dahliae. The first step in this analysis is the preparation of a sample of the interior of the bine (marrow). It contains the plant’s water-conducting vessels and thus possibly also Verticillium spores or 81
